AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039844.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039844.01.T01 MTR_7g080320 98.810 84 0 1 3 85 327 410 4.52e-41 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCACATCCATAAGATTTA+TGG 0.151914 7:+69680864 None:intergenic
GCTTAGGTGTTGCTAAATTA+TGG 0.257191 7:-69680750 MsG0780039844.01.T01:three_prime_UTR
AGCAGCTGCCATAAATCTTA+TGG 0.318878 7:-69680872 MsG0780039844.01.T01:three_prime_UTR
CCACGTTCACGGTCCCTACT+AGG 0.437974 7:+69681347 None:intergenic
AACGGAGTAGGGATCGTGAC+AGG 0.443961 7:-69681373 MsG0780039844.01.T01:CDS
CATTGTCTCGCCCTCGCTCA+TGG 0.461773 7:+69681318 None:intergenic
GTGGGTGTTGAACCTTGTCT+TGG 0.469863 7:-69680826 MsG0780039844.01.T01:three_prime_UTR
AGGGCAACCAAGTCGATGAC+AGG 0.489701 7:+69680635 None:intergenic
CATATCCACGGTCTCTATCA+TGG 0.494619 7:+69681267 None:intergenic
CATGGCTCTCACCACGTTCA+CGG 0.499053 7:+69681336 None:intergenic
ATCGTGACAGGGAGCCTAGT+AGG 0.501423 7:-69681361 MsG0780039844.01.T01:CDS
ATTGAACAATATCTGAATGA+TGG 0.531182 7:-69680784 MsG0780039844.01.T01:three_prime_UTR
TATGGTTTGATGTTGCTTTG+CGG 0.535034 7:-69680732 MsG0780039844.01.T01:three_prime_UTR
ACAGATCAAAAGAACGGAGT+AGG 0.538832 7:-69681385 MsG0780039844.01.T01:CDS
GATGGATGTAACTTGAGCTT+AGG 0.542349 7:-69680766 MsG0780039844.01.T01:three_prime_UTR
ATAAGATGTGTAGTCTAGGT+GGG 0.550978 7:-69680844 MsG0780039844.01.T01:three_prime_UTR
TCGTGACAGGGAGCCTAGTA+GGG 0.554514 7:-69681360 MsG0780039844.01.T01:CDS
ACCGTGGATATGATCGAGAT+CGG 0.556484 7:-69681256 MsG0780039844.01.T01:CDS
CAGATCAAAAGAACGGAGTA+GGG 0.561355 7:-69681384 MsG0780039844.01.T01:CDS
TGTGATAAGATGTGTAGTCT+AGG 0.567273 7:-69680848 MsG0780039844.01.T01:three_prime_UTR
CTAGAGATTATGATCGTCAC+AGG 0.572592 7:-69681172 MsG0780039844.01.T01:intron
CCGTGGATATGATCGAGATC+GGG 0.578018 7:-69681255 MsG0780039844.01.T01:CDS
GCGACTTCTTGAATCATAGC+GGG 0.581845 7:+69681220 None:intergenic
GTAGGAGCAGAGATCGTGAC+AGG 0.584050 7:-69681292 MsG0780039844.01.T01:CDS
GCTCTCGAGATCGTGACCTC+CGG 0.595184 7:+69681198 None:intergenic
AAAATTATTTGTCCAAGACA+AGG 0.599616 7:+69680814 None:intergenic
CCTAGTAGGGACCGTGAACG+TGG 0.608938 7:-69681347 MsG0780039844.01.T01:CDS
GTGGTGAGAGCCATGAGCGA+GGG 0.612251 7:-69681328 MsG0780039844.01.T01:CDS
GATAAGATGTGTAGTCTAGG+TGG 0.624297 7:-69680845 MsG0780039844.01.T01:three_prime_UTR
CCCGATCTCGATCATATCCA+CGG 0.629250 7:+69681255 None:intergenic
CGTGGTGAGAGCCATGAGCG+AGG 0.635589 7:-69681329 MsG0780039844.01.T01:CDS
ATATGCACAGATCAAAAGAA+CGG 0.645466 7:-69681391 MsG0780039844.01.T01:CDS
ACGGAGTAGGGATCGTGACA+GGG 0.652302 7:-69681372 MsG0780039844.01.T01:CDS
GGGCAACCAAGTCGATGACA+GGG 0.652657 7:+69680636 None:intergenic
GAGGGCGAGACAATGATCGT+AGG 0.664594 7:-69681310 MsG0780039844.01.T01:CDS
ATGATTCAAGAAGTCGCCGG+AGG 0.695304 7:-69681214 MsG0780039844.01.T01:CDS
AGGCACCATGATAGAGACCG+TGG 0.701940 7:-69681272 MsG0780039844.01.T01:CDS
GGCGACTTCTTGAATCATAG+CGG 0.719406 7:+69681219 None:intergenic
GCTATGATTCAAGAAGTCGC+CGG 0.727551 7:-69681217 MsG0780039844.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAAATTTTTATATTATCTA+TGG - Chr7:69681078-69681097 MsG0780039844.01.T01:intron 10.0%
! AAAATTATTTGTCCAAGACA+AGG + Chr7:69681214-69681233 None:intergenic 25.0%
! AGGAAGTAGTAATAAAGAAA+TGG + Chr7:69680894-69680913 None:intergenic 25.0%
! ATTGAACAATATCTGAATGA+TGG - Chr7:69681241-69681260 MsG0780039844.01.T01:CDS 25.0%
! TATCACATCCATAAGATTTA+TGG + Chr7:69681164-69681183 None:intergenic 25.0%
GATCAAATATGAGTGATACA+AGG - Chr7:69680961-69680980 MsG0780039844.01.T01:intron 30.0%
ATAAGATGTGTAGTCTAGGT+GGG - Chr7:69681181-69681200 MsG0780039844.01.T01:CDS 35.0%
CAAGCAAATTATACATCACG+AGG + Chr7:69681032-69681051 None:intergenic 35.0%
TATAGGAGTGACAATCAAGT+AGG + Chr7:69681004-69681023 None:intergenic 35.0%
TGTGATAAGATGTGTAGTCT+AGG - Chr7:69681177-69681196 MsG0780039844.01.T01:CDS 35.0%
! ACATTTGACCCCTTCTTTTT+GGG - Chr7:69680915-69680934 MsG0780039844.01.T01:intron 35.0%
!! GCTTAGGTGTTGCTAAATTA+TGG - Chr7:69681275-69681294 MsG0780039844.01.T01:CDS 35.0%
!! TATGGTTTGATGTTGCTTTG+CGG - Chr7:69681293-69681312 MsG0780039844.01.T01:CDS 35.0%
AAAAAGGCCCCAAAAAGAAG+GGG + Chr7:69680926-69680945 None:intergenic 40.0%
AAAAGAAGGGGTCAAATGTG+AGG + Chr7:69680914-69680933 None:intergenic 40.0%
ACAGATCAAAAGAACGGAGT+AGG - Chr7:69680640-69680659 MsG0780039844.01.T01:three_prime_UTR 40.0%
AGAAAAAGGCCCCAAAAAGA+AGG + Chr7:69680928-69680947 None:intergenic 40.0%
AGCAGCTGCCATAAATCTTA+TGG - Chr7:69681153-69681172 MsG0780039844.01.T01:intron 40.0%
CAGATCAAAAGAACGGAGTA+GGG - Chr7:69680641-69680660 MsG0780039844.01.T01:three_prime_UTR 40.0%
CTAGAGATTATGATCGTCAC+AGG - Chr7:69680853-69680872 MsG0780039844.01.T01:three_prime_UTR 40.0%
GAAAAAGGCCCCAAAAAGAA+GGG + Chr7:69680927-69680946 None:intergenic 40.0%
GATAAGATGTGTAGTCTAGG+TGG - Chr7:69681180-69681199 MsG0780039844.01.T01:CDS 40.0%
GATGGATGTAACTTGAGCTT+AGG - Chr7:69681259-69681278 MsG0780039844.01.T01:CDS 40.0%
TACATCACGAGGAACACTAT+AGG + Chr7:69681021-69681040 None:intergenic 40.0%
TATGAGTGATACAAGGTTCC+AGG - Chr7:69680968-69680987 MsG0780039844.01.T01:intron 40.0%
! CACATTTGACCCCTTCTTTT+TGG - Chr7:69680914-69680933 MsG0780039844.01.T01:intron 40.0%
! CATTTGACCCCTTCTTTTTG+GGG - Chr7:69680916-69680935 MsG0780039844.01.T01:intron 40.0%
! CGTTCTTTTGATCTGTGCAT+GGG + Chr7:69680636-69680655 None:intergenic 40.0%
ACCGTGGATATGATCGAGAT+CGG - Chr7:69680769-69680788 MsG0780039844.01.T01:three_prime_UTR 45.0%
CATATCCACGGTCTCTATCA+TGG + Chr7:69680761-69680780 None:intergenic 45.0%
CCACTCGCACTTGTAGAAAA+AGG + Chr7:69680942-69680961 None:intergenic 45.0%
GCGACTTCTTGAATCATAGC+GGG + Chr7:69680808-69680827 None:intergenic 45.0%
GCTATGATTCAAGAAGTCGC+CGG - Chr7:69680808-69680827 MsG0780039844.01.T01:three_prime_UTR 45.0%
GGCGACTTCTTGAATCATAG+CGG + Chr7:69680809-69680828 None:intergenic 45.0%
! CCGTTCTTTTGATCTGTGCA+TGG + Chr7:69680637-69680656 None:intergenic 45.0%
! CCTTTTTCTACAAGTGCGAG+TGG - Chr7:69680939-69680958 MsG0780039844.01.T01:intron 45.0%
!! TGTTTTCCCTGTCATCGACT+TGG - Chr7:69681383-69681402 MsG0780039844.01.T01:CDS 45.0%
ATGATTCAAGAAGTCGCCGG+AGG - Chr7:69680811-69680830 MsG0780039844.01.T01:three_prime_UTR 50.0%
CAAGTAGGCACTACTGATCC+TGG + Chr7:69680989-69681008 None:intergenic 50.0%
CAGCAAGACAGGTTAGTACG+AGG + Chr7:69681107-69681126 None:intergenic 50.0%
CCCGATCTCGATCATATCCA+CGG + Chr7:69680773-69680792 None:intergenic 50.0%
CCGTGGATATGATCGAGATC+GGG - Chr7:69680770-69680789 MsG0780039844.01.T01:three_prime_UTR 50.0%
! GTTTTGCCAGGCGTCATGAT+CGG - Chr7:69681126-69681145 MsG0780039844.01.T01:intron 50.0%
!! GTGGGTGTTGAACCTTGTCT+TGG - Chr7:69681199-69681218 MsG0780039844.01.T01:CDS 50.0%
!!! ACCTGTCTTGCTGTTTTGCC+AGG - Chr7:69681114-69681133 MsG0780039844.01.T01:intron 50.0%
AACGGAGTAGGGATCGTGAC+AGG - Chr7:69680652-69680671 MsG0780039844.01.T01:three_prime_UTR 55.0%
ACGGAGTAGGGATCGTGACA+GGG - Chr7:69680653-69680672 MsG0780039844.01.T01:three_prime_UTR 55.0%
AGGCACCATGATAGAGACCG+TGG - Chr7:69680753-69680772 MsG0780039844.01.T01:three_prime_UTR 55.0%
ATCGTGACAGGGAGCCTAGT+AGG - Chr7:69680664-69680683 MsG0780039844.01.T01:three_prime_UTR 55.0%
CATGGCTCTCACCACGTTCA+CGG + Chr7:69680692-69680711 None:intergenic 55.0%
GAGGGCGAGACAATGATCGT+AGG - Chr7:69680715-69680734 MsG0780039844.01.T01:three_prime_UTR 55.0%
GCCTGGCAAAACAGCAAGAC+AGG + Chr7:69681118-69681137 None:intergenic 55.0%
GGGCAACCAAGTCGATGACA+GGG + Chr7:69681392-69681411 None:intergenic 55.0%
GTAGGAGCAGAGATCGTGAC+AGG - Chr7:69680733-69680752 MsG0780039844.01.T01:three_prime_UTR 55.0%
TCGTGACAGGGAGCCTAGTA+GGG - Chr7:69680665-69680684 MsG0780039844.01.T01:three_prime_UTR 55.0%
!! CTAGTACCGATCATGACGCC+TGG + Chr7:69681135-69681154 None:intergenic 55.0%
CATTGTCTCGCCCTCGCTCA+TGG + Chr7:69680710-69680729 None:intergenic 60.0%
CCACGTTCACGGTCCCTACT+AGG + Chr7:69680681-69680700 None:intergenic 60.0%
CCTAGTAGGGACCGTGAACG+TGG - Chr7:69680678-69680697 MsG0780039844.01.T01:three_prime_UTR 60.0%
GCTCTCGAGATCGTGACCTC+CGG + Chr7:69680830-69680849 None:intergenic 60.0%
GTGGTGAGAGCCATGAGCGA+GGG - Chr7:69680697-69680716 MsG0780039844.01.T01:three_prime_UTR 60.0%
CGTGGTGAGAGCCATGAGCG+AGG - Chr7:69680696-69680715 MsG0780039844.01.T01:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 69680636 69681411 69680636 ID=MsG0780039844.01;Name=MsG0780039844.01
Chr7 mRNA 69680636 69681411 69680636 ID=MsG0780039844.01.T01;Parent=MsG0780039844.01;Name=MsG0780039844.01.T01;_AED=0.46;_eAED=0.46;_QI=0|1|0.5|1|0|0|2|257|85
Chr7 exon 69680636 69680911 69680636 ID=MsG0780039844.01.T01:exon:12716;Parent=MsG0780039844.01.T01
Chr7 exon 69681173 69681411 69681173 ID=MsG0780039844.01.T01:exon:12715;Parent=MsG0780039844.01.T01
Chr7 CDS 69681173 69681411 69681173 ID=MsG0780039844.01.T01:cds;Parent=MsG0780039844.01.T01
Chr7 CDS 69680893 69680911 69680893 ID=MsG0780039844.01.T01:cds;Parent=MsG0780039844.01.T01
Chr7 three_prime_UTR 69680636 69680892 69680636 ID=MsG0780039844.01.T01:three_prime_utr;Parent=MsG0780039844.01.T01
Gene Sequence

>MsG0780039844.01.T01

ATGCACAGATCAAAAGAACGGAGTAGGGATCGTGACAGGGAGCCTAGTAGGGACCGTGAACGTGGTGAGAGCCATGAGCGAGGGCGAGACAATGATCGTAGGAGCAGAGATCGTGACAGGCACCATGATAGAGACCGTGGATATGATCGAGATCGGGACAGAGACTCATCCCGCTATGATTCAAGAAGTCGCCGGAGGTCACGATCTCGAGAGCGTTCTAGAGATTATGATCGTCACAGGCGTCATGATCGGTACTAG

Protein sequence

>MsG0780039844.01.T01

MHRSKERSRDRDREPSRDRERGESHERGRDNDRRSRDRDRHHDRDRGYDRDRDRDSSRYDSRSRRRSRSRERSRDYDRHRRHDRY*