Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046126.01.T01 | XP_003608667.1 | 98.592 | 71 | 1 | 0 | 7 | 77 | 148 | 218 | 1.32E-40 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046126.01.T01 | Q93VG8 | 47.143 | 70 | 34 | 1 | 7 | 76 | 152 | 218 | 8.86E-14 | 66.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046126.01.T01 | G7JHP3 | 98.592 | 71 | 1 | 0 | 7 | 77 | 148 | 218 | 6.31e-41 | 142 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048135.01 | MsG0880046126.01 | 0.801399 | 9.171097e-49 | 4.375334e-46 |
| MsG0080048817.01 | MsG0880046126.01 | 0.818160 | 2.265273e-52 | 1.682677e-49 |
| MsG0080049013.01 | MsG0880046126.01 | -0.804353 | 2.250020e-49 | 1.157596e-46 |
| MsG0180000152.01 | MsG0880046126.01 | 0.866518 | 2.688680e-65 | 9.088021e-62 |
| MsG0180000544.01 | MsG0880046126.01 | -0.805643 | 1.208812e-49 | 6.429158e-47 |
| MsG0180000550.01 | MsG0880046126.01 | 0.803546 | 3.311219e-49 | 1.668643e-46 |
| MsG0180000614.01 | MsG0880046126.01 | 0.874034 | 9.252171e-68 | 4.102506e-64 |
| MsG0180000615.01 | MsG0880046126.01 | 0.836294 | 1.018387e-56 | 1.278029e-53 |
| MsG0180000679.01 | MsG0880046126.01 | 0.809764 | 1.610562e-50 | 9.538813e-48 |
| MsG0180000743.01 | MsG0880046126.01 | 0.821981 | 3.023650e-53 | 2.497901e-50 |
| MsG0180001131.01 | MsG0880046126.01 | 0.802528 | 5.375241e-49 | 2.639029e-46 |
| MsG0180001141.01 | MsG0880046126.01 | 0.807443 | 5.043420e-50 | 2.809408e-47 |
| MsG0180001213.01 | MsG0880046126.01 | 0.800044 | 1.733329e-48 | 7.990474e-46 |
| MsG0180001795.01 | MsG0880046126.01 | 0.843261 | 1.562252e-58 | 2.430664e-55 |
| MsG0180002458.01 | MsG0880046126.01 | 0.811569 | 6.558165e-51 | 4.073548e-48 |
| MsG0180002482.01 | MsG0880046126.01 | 0.882231 | 1.239030e-70 | 7.488544e-67 |
| MsG0180002504.01 | MsG0880046126.01 | 0.822963 | 1.788162e-53 | 1.519161e-50 |
| MsG0880044394.01 | MsG0880046126.01 | 0.822727 | 2.029591e-53 | 1.712863e-50 |
| MsG0880045042.01 | MsG0880046126.01 | 0.810184 | 1.307844e-50 | 7.830694e-48 |
| MsG0880045555.01 | MsG0880046126.01 | -0.829308 | 5.536993e-55 | 5.646424e-52 |
| MsG0880045704.01 | MsG0880046126.01 | -0.804351 | 2.252740e-49 | 1.158912e-46 |
| MsG0880046126.01 | MsG0880047165.01 | 0.827247 | 1.737895e-54 | 1.668563e-51 |
| MsG0880046126.01 | MsG0880047427.01 | 0.831430 | 1.676557e-55 | 1.818864e-52 |
| MsG0880046126.01 | MsG0880047448.01 | 0.842471 | 2.533568e-58 | 3.845812e-55 |
| MsG0180003124.01 | MsG0880046126.01 | 0.810226 | 1.280657e-50 | 7.676254e-48 |
| MsG0180003952.01 | MsG0880046126.01 | 0.812295 | 4.555153e-51 | 2.885210e-48 |
| MsG0180004259.01 | MsG0880046126.01 | 0.800821 | 1.204029e-48 | 5.660942e-46 |
| MsG0180004451.01 | MsG0880046126.01 | -0.811521 | 6.717432e-51 | 4.167301e-48 |
| MsG0180004820.01 | MsG0880046126.01 | 0.822743 | 2.012259e-53 | 1.699019e-50 |
| MsG0180004886.01 | MsG0880046126.01 | -0.806985 | 6.303431e-50 | 3.469795e-47 |
| MsG0180005193.01 | MsG0880046126.01 | 0.847161 | 1.377296e-59 | 2.426540e-56 |
| MsG0180005334.01 | MsG0880046126.01 | 0.829578 | 4.759368e-55 | 4.890906e-52 |
| MsG0180005424.01 | MsG0880046126.01 | 0.810106 | 1.359476e-50 | 8.123859e-48 |
| MsG0180005851.01 | MsG0880046126.01 | 0.831609 | 1.515002e-55 | 1.652497e-52 |
| MsG0180006144.01 | MsG0880046126.01 | 0.844095 | 9.348764e-59 | 1.493185e-55 |
| MsG0180006220.01 | MsG0880046126.01 | 0.831683 | 1.452438e-55 | 1.587750e-52 |
| MsG0280006422.01 | MsG0880046126.01 | 0.825764 | 3.922491e-54 | 3.608818e-51 |
| MsG0780040858.01 | MsG0880046126.01 | 0.888453 | 5.827451e-73 | 4.517184e-69 |
| MsG0780041223.01 | MsG0880046126.01 | 0.806196 | 9.252298e-50 | 4.991083e-47 |
| MsG0780041402.01 | MsG0880046126.01 | 0.822539 | 2.244463e-53 | 1.884135e-50 |
| MsG0780041507.01 | MsG0880046126.01 | 0.808206 | 3.470068e-50 | 1.972156e-47 |
| MsG0880042131.01 | MsG0880046126.01 | 0.829917 | 3.936173e-55 | 4.085341e-52 |
| MsG0880042294.01 | MsG0880046126.01 | 0.811926 | 5.481808e-51 | 3.437258e-48 |
| MsG0880042779.01 | MsG0880046126.01 | 0.801585 | 8.401362e-49 | 4.026937e-46 |
| MsG0880043303.01 | MsG0880046126.01 | 0.825550 | 4.410910e-54 | 4.033199e-51 |
| MsG0880043465.01 | MsG0880046126.01 | 0.806929 | 6.479710e-50 | 3.561630e-47 |
| MsG0880043905.01 | MsG0880046126.01 | 0.827462 | 1.543787e-54 | 1.491515e-51 |
| MsG0280010776.01 | MsG0880046126.01 | 0.803161 | 3.979123e-49 | 1.985419e-46 |
| MsG0280011388.01 | MsG0880046126.01 | 0.804462 | 2.135415e-49 | 1.101694e-46 |
| MsG0280011421.01 | MsG0880046126.01 | 0.805664 | 1.196731e-49 | 6.368287e-47 |
| MsG0380012661.01 | MsG0880046126.01 | -0.861158 | 1.248908e-63 | 3.499661e-60 |
| MsG0380014647.01 | MsG0880046126.01 | 0.858974 | 5.697559e-63 | 1.480902e-59 |
| MsG0680032430.01 | MsG0880046126.01 | 0.809580 | 1.764048e-50 | 1.039697e-47 |
| MsG0480018497.01 | MsG0880046126.01 | -0.804774 | 1.838403e-49 | 9.561582e-47 |
| MsG0480018603.01 | MsG0880046126.01 | 0.816812 | 4.560222e-52 | 3.264469e-49 |
| MsG0480018906.01 | MsG0880046126.01 | 0.825313 | 5.017684e-54 | 4.557355e-51 |
| MsG0480019163.01 | MsG0880046126.01 | -0.815650 | 8.293564e-52 | 5.751579e-49 |
| MsG0480020136.01 | MsG0880046126.01 | 0.806681 | 7.310816e-50 | 3.992630e-47 |
| MsG0480020874.01 | MsG0880046126.01 | 0.825786 | 3.875830e-54 | 3.568168e-51 |
| MsG0480021251.01 | MsG0880046126.01 | 0.819480 | 1.135823e-52 | 8.751128e-50 |
| MsG0480021448.01 | MsG0880046126.01 | 0.845656 | 3.545199e-59 | 5.950305e-56 |
| MsG0480021451.01 | MsG0880046126.01 | 0.846702 | 1.839409e-59 | 3.193456e-56 |
| MsG0480021453.01 | MsG0880046126.01 | 0.856488 | 3.110903e-62 | 7.438343e-59 |
| MsG0480021750.01 | MsG0880046126.01 | 0.833362 | 5.572405e-56 | 6.402909e-53 |
| MsG0480021994.01 | MsG0880046126.01 | 0.808081 | 3.690025e-50 | 2.090130e-47 |
| MsG0480022035.01 | MsG0880046126.01 | 0.816916 | 4.320535e-52 | 3.101777e-49 |
| MsG0480022052.01 | MsG0880046126.01 | -0.824247 | 8.948687e-54 | 7.882939e-51 |
| MsG0480022222.01 | MsG0880046126.01 | 0.832200 | 1.082518e-55 | 1.201415e-52 |
| MsG0480022340.01 | MsG0880046126.01 | -0.816813 | 4.557381e-52 | 3.262534e-49 |
| MsG0480022707.01 | MsG0880046126.01 | 0.813254 | 2.809363e-51 | 1.825342e-48 |
| MsG0480022768.01 | MsG0880046126.01 | 0.841741 | 3.954230e-58 | 5.864682e-55 |
| MsG0480022795.01 | MsG0880046126.01 | 0.864219 | 1.422728e-64 | 4.433000e-61 |
| MsG0580024051.01 | MsG0880046126.01 | 0.825249 | 5.196674e-54 | 4.711260e-51 |
| MsG0580024468.01 | MsG0880046126.01 | 0.853844 | 1.826374e-61 | 4.000191e-58 |
| MsG0380015027.01 | MsG0880046126.01 | 0.863125 | 3.111583e-64 | 9.331216e-61 |
| MsG0380015400.01 | MsG0880046126.01 | 0.813243 | 2.824932e-51 | 1.834901e-48 |
| MsG0380015466.01 | MsG0880046126.01 | 0.838161 | 3.390105e-57 | 4.502010e-54 |
| MsG0380016004.01 | MsG0880046126.01 | 0.801940 | 7.104392e-49 | 3.436100e-46 |
| MsG0380016547.01 | MsG0880046126.01 | -0.801300 | 9.608744e-49 | 4.572917e-46 |
| MsG0380016559.01 | MsG0880046126.01 | 0.831043 | 2.086919e-55 | 2.238276e-52 |
| MsG0380016560.01 | MsG0880046126.01 | 0.834460 | 2.958671e-56 | 3.513712e-53 |
| MsG0380016828.01 | MsG0880046126.01 | -0.807417 | 5.105905e-50 | 2.842401e-47 |
| MsG0380016886.01 | MsG0880046126.01 | 0.885266 | 9.427348e-72 | 6.427869e-68 |
| MsG0380017254.01 | MsG0880046126.01 | 0.801794 | 7.611227e-49 | 3.667687e-46 |
| MsG0380017776.01 | MsG0880046126.01 | -0.812296 | 4.553712e-51 | 2.884350e-48 |
| MsG0480018104.01 | MsG0880046126.01 | 0.817722 | 2.846265e-52 | 2.089018e-49 |
| MsG0480018110.01 | MsG0880046126.01 | 0.835020 | 2.139960e-56 | 2.584090e-53 |
| MsG0480018214.01 | MsG0880046126.01 | 0.816111 | 6.542866e-52 | 4.594977e-49 |
| MsG0580028044.01 | MsG0880046126.01 | 0.819744 | 9.890870e-53 | 7.676537e-50 |
| MsG0580029014.01 | MsG0880046126.01 | 0.809984 | 1.443990e-50 | 8.601633e-48 |
| MsG0580029166.01 | MsG0880046126.01 | 0.815494 | 8.981048e-52 | 6.201853e-49 |
| MsG0580029717.01 | MsG0880046126.01 | 0.821368 | 4.191418e-53 | 3.403261e-50 |
| MsG0680030415.01 | MsG0880046126.01 | 0.812904 | 3.353219e-51 | 2.158344e-48 |
| MsG0280006483.01 | MsG0880046126.01 | 0.821343 | 4.246295e-53 | 3.445522e-50 |
| MsG0280007058.01 | MsG0880046126.01 | -0.857805 | 1.270696e-62 | 3.174685e-59 |
| MsG0280007787.01 | MsG0880046126.01 | 0.818045 | 2.405346e-52 | 1.781024e-49 |
| MsG0280009109.01 | MsG0880046126.01 | 0.828528 | 8.549660e-55 | 8.520476e-52 |
| MsG0680035659.01 | MsG0880046126.01 | 0.846394 | 2.233270e-59 | 3.838511e-56 |
| MsG0780036546.01 | MsG0880046126.01 | 0.822780 | 1.972855e-53 | 1.667453e-50 |
| MsG0780038400.01 | MsG0880046126.01 | 0.810623 | 1.051178e-50 | 6.367814e-48 |
| MsG0780038911.01 | MsG0880046126.01 | 0.817466 | 3.250210e-52 | 2.368920e-49 |
| MsG0780039841.01 | MsG0880046126.01 | 0.828105 | 1.081846e-54 | 1.064966e-51 |
| MsG0780039844.01 | MsG0880046126.01 | 0.832546 | 8.887759e-56 | 9.966224e-53 |
| MsG0780040038.01 | MsG0880046126.01 | 0.825732 | 3.993355e-54 | 3.670568e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046126.01.T01 | MTR_4g100500 | 98.592 | 71 | 1 | 0 | 7 | 77 | 148 | 218 | 1.60e-44 | 142 |
| MsG0880046126.01.T01 | MTR_5g013040 | 57.746 | 71 | 29 | 1 | 7 | 76 | 137 | 207 | 1.71e-14 | 65.1 |
| MsG0880046126.01.T01 | MTR_5g013040 | 57.746 | 71 | 29 | 1 | 7 | 76 | 146 | 216 | 2.04e-14 | 65.1 |
| MsG0880046126.01.T01 | MTR_5g013040 | 57.746 | 71 | 29 | 1 | 7 | 76 | 149 | 219 | 2.38e-14 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046126.01.T01 | AT4G17486 | 47.143 | 70 | 34 | 1 | 7 | 76 | 152 | 218 | 9.03e-15 | 66.2 |
| MsG0880046126.01.T01 | AT5G47310 | 41.667 | 72 | 39 | 2 | 7 | 76 | 154 | 224 | 1.69e-11 | 57.8 |
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTCAACCTGAAGGCTTTC+TGG | 0.145473 | 8:-69397671 | None:intergenic |
| CAAATGCAGTTTGAAGTTTC+AGG | 0.202280 | 8:+69397626 | None:intergenic |
| CATTGGTCTTTCCTTTATAA+AGG | 0.295183 | 8:-69398028 | None:intergenic |
| TGAATTTAGCAAATGGTGTT+TGG | 0.374640 | 8:-69397989 | None:intergenic |
| TGGTGATGTCTCCTTTATAA+AGG | 0.375695 | 8:+69398017 | MsG0880046126.01.T01:CDS |
| TCTTCTGCCAGAAAGCCTTC+AGG | 0.380629 | 8:+69397664 | MsG0880046126.01.T01:CDS |
| CCTGATCATCTTATACATTC+TGG | 0.383600 | 8:+69397707 | MsG0880046126.01.T01:CDS |
| CATTTGCTAAATTCACATAA+TGG | 0.396106 | 8:+69397997 | MsG0880046126.01.T01:CDS |
| CCAGAATGTATAAGATGATC+AGG | 0.430572 | 8:-69397707 | None:intergenic |
| GCCAGAAAGCCTTCAGGTTG+AGG | 0.433257 | 8:+69397670 | MsG0880046126.01.T01:CDS |
| CATTATGTGAATTTAGCAAA+TGG | 0.485305 | 8:-69397996 | None:intergenic |
| CACTATCATCTGACTCAGAA+TGG | 0.519123 | 8:-69397954 | None:intergenic |
| GAAAATCATTCGACGTCCAT+TGG | 0.519796 | 8:-69398045 | None:intergenic |
| ATTTGTTACTGCAGATGAAG+AGG | 0.537451 | 8:+69397915 | MsG0880046126.01.T01:intron |
| TGAGTCAGATGATAGTGAAG+AGG | 0.577666 | 8:+69397960 | MsG0880046126.01.T01:CDS |
| GTCTAACAGCCTCAACCTGA+AGG | 0.593173 | 8:-69397679 | None:intergenic |
| TCAGATGATAGTGAAGAGGA+AGG | 0.593790 | 8:+69397964 | MsG0880046126.01.T01:CDS |
| CTTTATAAAGGAAAGACCAA+TGG | 0.604885 | 8:+69398029 | MsG0880046126.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTATCTTTTTTTTTTTAAT+TGG | + | Chr8:69397868-69397887 | MsG0880046126.01.T01:intron | 10.0% |
| !! | GAAAAAAAATCTAATATGAA+TGG | + | Chr8:69397814-69397833 | MsG0880046126.01.T01:intron | 15.0% |
| ! | CATTATGTGAATTTAGCAAA+TGG | - | Chr8:69397999-69398018 | None:intergenic | 25.0% |
| !! | CATTTGCTAAATTCACATAA+TGG | + | Chr8:69397997-69398016 | MsG0880046126.01.T01:CDS | 25.0% |
| !! | TAATTGGATAGTAACACTTT+TGG | + | Chr8:69397884-69397903 | MsG0880046126.01.T01:intron | 25.0% |
| !!! | CTACTAATTGTTTTACATTC+TGG | + | Chr8:69397756-69397775 | MsG0880046126.01.T01:intron | 25.0% |
| !!! | TATCTTTCAGATTGATTTGT+TGG | + | Chr8:69397779-69397798 | MsG0880046126.01.T01:intron | 25.0% |
| CTTTATAAAGGAAAGACCAA+TGG | + | Chr8:69398029-69398048 | MsG0880046126.01.T01:CDS | 30.0% | |
| TGAATTTAGCAAATGGTGTT+TGG | - | Chr8:69397992-69398011 | None:intergenic | 30.0% | |
| TTATACATTCTGGTAAGCTA+TGG | + | Chr8:69397717-69397736 | MsG0880046126.01.T01:intron | 30.0% | |
| !! | CATTGGTCTTTCCTTTATAA+AGG | - | Chr8:69398031-69398050 | None:intergenic | 30.0% |
| CCAGAATGTATAAGATGATC+AGG | - | Chr8:69397710-69397729 | None:intergenic | 35.0% | |
| CCTGATCATCTTATACATTC+TGG | + | Chr8:69397707-69397726 | MsG0880046126.01.T01:CDS | 35.0% | |
| ! | ATTTGTTACTGCAGATGAAG+AGG | + | Chr8:69397915-69397934 | MsG0880046126.01.T01:intron | 35.0% |
| ! | TGGTGATGTCTCCTTTATAA+AGG | + | Chr8:69398017-69398036 | MsG0880046126.01.T01:CDS | 35.0% |
| CACTATCATCTGACTCAGAA+TGG | - | Chr8:69397957-69397976 | None:intergenic | 40.0% | |
| ! | TCAGATGATAGTGAAGAGGA+AGG | + | Chr8:69397964-69397983 | MsG0880046126.01.T01:CDS | 40.0% |
| ! | TGAGTCAGATGATAGTGAAG+AGG | + | Chr8:69397960-69397979 | MsG0880046126.01.T01:CDS | 40.0% |
| GTCTAACAGCCTCAACCTGA+AGG | - | Chr8:69397682-69397701 | None:intergenic | 50.0% | |
| TCTTCTGCCAGAAAGCCTTC+AGG | + | Chr8:69397664-69397683 | MsG0880046126.01.T01:CDS | 50.0% | |
| GCCTCAACCTGAAGGCTTTC+TGG | - | Chr8:69397674-69397693 | None:intergenic | 55.0% | |
| !! | GCCAGAAAGCCTTCAGGTTG+AGG | + | Chr8:69397670-69397689 | MsG0880046126.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 69397629 | 69398062 | 69397629 | ID=MsG0880046126.01;Name=MsG0880046126.01 |
| Chr8 | mRNA | 69397629 | 69398062 | 69397629 | ID=MsG0880046126.01.T01;Parent=MsG0880046126.01;Name=MsG0880046126.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0|0|1|1|1|2|0|77 |
| Chr8 | exon | 69397629 | 69397728 | 69397629 | ID=MsG0880046126.01.T01:exon:12968;Parent=MsG0880046126.01.T01 |
| Chr8 | exon | 69397929 | 69398062 | 69397929 | ID=MsG0880046126.01.T01:exon:12969;Parent=MsG0880046126.01.T01 |
| Chr8 | CDS | 69397629 | 69397728 | 69397629 | ID=MsG0880046126.01.T01:cds;Parent=MsG0880046126.01.T01 |
| Chr8 | CDS | 69397929 | 69398062 | 69397929 | ID=MsG0880046126.01.T01:cds;Parent=MsG0880046126.01.T01 |
| Gene Sequence |
| Protein sequence |
