AlfalfaGEDB | MsG0180005552.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005552.01

KEGG: -
GO: GO:0016491 GO:0055114
PFAM: PF00248

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005552.01.T01	XP_039685887.1	89.504	343	35	1	1	342	1	343	0	630

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005552.01.T01	Q3L181	66.866	335	110	1	8	341	1	335	2.05E-170	480

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005552.01.T01	A0A2K3PG96	88.921	343	37	1	1	342	1	343	0.0	625

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080047875.01	MsG0180005552.01	PPI
MsG0180001976.01	MsG0180005552.01	PPI
MsG0180001976.01	MsG0180005552.01	PPI
MsG0180005552.01	MsG0180001985.01	PPI
MsG0180005552.01	MsG0180001772.01	PPI
MsG0180005553.01	MsG0180005552.01	PPI
MsG0280008313.01	MsG0180005552.01	PPI
MsG0280009794.01	MsG0180005552.01	PPI
MsG0380016623.01	MsG0180005552.01	PPI
MsG0480019714.01	MsG0180005552.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005552.01.T01	MTR_1g102750	88.496	339	38	1	5	342	2	340	0.0	617
MsG0180005552.01.T01	MTR_1g102750	90.141	284	27	1	5	287	2	285	0.0	529
MsG0180005552.01.T01	MTR_7g114980	65.089	338	114	1	9	342	12	349	1.03e-168	473
MsG0180005552.01.T01	MTR_7g114970	62.832	339	123	3	6	342	5	342	2.85e-163	459
MsG0180005552.01.T01	MTR_7g114990	62.941	340	123	2	3	342	9	345	3.14e-163	460
MsG0180005552.01.T01	MTR_7g115010	59.064	342	122	3	3	342	5	330	9.49e-149	422
MsG0180005552.01.T01	MTR_7g114980	66.548	281	90	1	9	285	12	292	1.62e-142	405
MsG0180005552.01.T01	MTR_7g114970	64.539	282	97	3	6	285	5	285	2.60e-138	394
MsG0180005552.01.T01	MTR_7g114970	64.413	281	97	3	6	284	5	284	3.95e-137	391
MsG0180005552.01.T01	MTR_7g021850	57.612	335	135	3	8	341	4	332	7.25e-137	392

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005552.01.T01	AT1G60710	57.015	335	140	3	8	341	7	338	5.33e-133	383
MsG0180005552.01.T01	AT1G60690	55.357	336	146	3	7	341	6	338	4.48e-131	378
MsG0180005552.01.T01	AT1G60680	55.786	337	144	4	7	341	6	339	4.74e-131	378
MsG0180005552.01.T01	AT1G60730	55.522	335	145	3	8	341	7	338	4.02e-129	373
MsG0180005552.01.T01	AT1G60750	54.971	342	134	4	1	341	1	323	5.77e-129	372
MsG0180005552.01.T01	AT1G10810	55.357	336	144	5	8	341	7	338	4.79e-126	365
MsG0180005552.01.T01	AT1G60730	52.394	355	145	4	8	341	7	358	3.12e-124	361
MsG0180005552.01.T01	AT1G10810	56.929	267	112	2	76	341	22	286	1.35e-104	309
MsG0180005552.01.T01	AT1G60680	64.651	215	74	2	7	220	6	219	1.27e-94	281
MsG0180005552.01.T01	AT1G10810	58.974	234	92	4	8	239	7	238	2.82e-89	268

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Find 101 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTTTACAAGAGAAAA+GGG	0.493496	1:+93198685	MsG0180005552.01.T01:three_prime_UTR
AACATCCATCTTCATGGGAG+AGG	0.535281	1:-93196504	None:intergenic
AAGAAATAATTCCACTCTGC+AGG	0.468173	1:+93197349	MsG0180005552.01.T01:CDS
AAGAACTTTGAAAACAACAT+TGG	0.532327	1:+93198467	MsG0180005552.01.T01:CDS
AAGAGAAAAGGGCACAGTTA+AGG	0.373822	1:+93198696	MsG0180005552.01.T01:three_prime_UTR
AAGCTTACGGTGTCTTCAAT+TGG	0.376667	1:-93197078	None:intergenic
AAGGAAAGATAAGACATATT+GGG	0.422532	1:+93197226	MsG0180005552.01.T01:CDS
AAGGTACCCCTGAATATGTG+AGG	0.623139	1:+93196974	MsG0180005552.01.T01:CDS
AATAGGACAATTGCATGTTG+TGG	0.576672	1:+93198820	MsG0180005552.01.T01:three_prime_UTR
AATATGCATCCCAGGTTCAC+TGG	0.452442	1:+93197859	MsG0180005552.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACTATGCCAAAGCTAA+AGG	-	Chr1:93195495-93195514	None:intergenic	30.0%
AACTAACTGGAGTATAATTG+TGG	-	Chr1:93197414-93197433	None:intergenic	30.0%
AATGTTTGTGGATTCTGATA+GGG	+	Chr1:93196392-93196411	MsG0180005552.01.T01:intron	30.0%
AATTCCTTAGATGTGACAAA+TGG	+	Chr1:93196753-93196772	MsG0180005552.01.T01:intron	30.0%
AATTGAACATCCATCTTCAT+GGG	-	Chr1:93196512-93196531	None:intergenic	30.0%
ACTTTAATGAGATAAGGTTC+AGG	+	Chr1:93197727-93197746	MsG0180005552.01.T01:intron	30.0%
AGTTAGGGTGTGTAATAAAT+AGG	+	Chr1:93198803-93198822	MsG0180005552.01.T01:three_prime_UTR	30.0%
ATATTATTAAGACAGTACCG+GGG	-	Chr1:93198011-93198030	None:intergenic	30.0%
ATGCTTGATTGAAGAAAACA+AGG	+	Chr1:93195896-93195915	MsG0180005552.01.T01:intron	30.0%
ATTCCTTAGATGTGACAAAT+GGG	+	Chr1:93196754-93196773	MsG0180005552.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	93195487	93198972	93195487	ID=MsG0180005552.01;Name=MsG0180005552.01
Chr1	mRNA	93195487	93198972	93195487	ID=MsG0180005552.01.T01;Parent=MsG0180005552.01;Name=MsG0180005552.01.T01;_AED=0.50;_eAED=0.50;_QI=102\|1\|1\|1\|1\|1\|7\|344\|342
Chr1	exon	93195487	93195645	93195487	ID=MsG0180005552.01.T01:exon:16039;Parent=MsG0180005552.01.T01
Chr1	exon	93196451	93196621	93196451	ID=MsG0180005552.01.T01:exon:16040;Parent=MsG0180005552.01.T01
Chr1	exon	93196892	93197092	93196892	ID=MsG0180005552.01.T01:exon:16041;Parent=MsG0180005552.01.T01
Chr1	exon	93197198	93197370	93197198	ID=MsG0180005552.01.T01:exon:16042;Parent=MsG0180005552.01.T01
Chr1	exon	93197547	93197643	93197547	ID=MsG0180005552.01.T01:exon:16043;Parent=MsG0180005552.01.T01
Chr1	exon	93197859	93198012	93197859	ID=MsG0180005552.01.T01:exon:16044;Parent=MsG0180005552.01.T01
Chr1	exon	93198453	93198972	93198453	ID=MsG0180005552.01.T01:exon:16045;Parent=MsG0180005552.01.T01
Chr1	five_prime_UTR	93195487	93195588	93195487	ID=MsG0180005552.01.T01:five_prime_utr;Parent=MsG0180005552.01.T01
Chr1	CDS	93195589	93195645	93195589	ID=MsG0180005552.01.T01:cds;Parent=MsG0180005552.01.T01
Chr1	CDS	93196451	93196621	93196451	ID=MsG0180005552.01.T01:cds;Parent=MsG0180005552.01.T01
Chr1	CDS	93196892	93197092	93196892	ID=MsG0180005552.01.T01:cds;Parent=MsG0180005552.01.T01
Chr1	CDS	93197198	93197370	93197198	ID=MsG0180005552.01.T01:cds;Parent=MsG0180005552.01.T01
Chr1	CDS	93197547	93197643	93197547	ID=MsG0180005552.01.T01:cds;Parent=MsG0180005552.01.T01
Chr1	CDS	93197859	93198012	93197859	ID=MsG0180005552.01.T01:cds;Parent=MsG0180005552.01.T01
Chr1	CDS	93198453	93198628	93198453	ID=MsG0180005552.01.T01:cds;Parent=MsG0180005552.01.T01
Chr1	three_prime_UTR	93198629	93198972	93198629	ID=MsG0180005552.01.T01:three_prime_utr;Parent=MsG0180005552.01.T01

Gene Sequence

>MsG0180005552.01.T01

ATGGATAAGACTGAGATGCAAGTCCCAAGAGTTAAACTGGGTAGCCATGGTTTAGAGGTGTCTAGGTTGGGATTTGGATGTGGAGGATTATCTGGAATTTTTAATGCTCCCCTCTCCCATGAAGATGGATGTTCAATTATTAAGGAAGTATTCAATAGAGGGGTTACCTTTTTCGATACATCAGATCTCTACGGAGACAATCATGATAATGAAATCATGGTTGGTAAGGCATTAAAAGAACTTCCCCGCGAAAAAGTTCAATTGGCTACAAAGTTTGGCGTTACTTTATCTGATGGGGTTTTTAGAGTAAAAGGTACCCCTGAATATGTGAGGGAGTGCTGTGAAGCGAGTCTCAAGCGGCTTGATGTCAATTATATTGACTTATACTATCAACACCGAGTTGACACTTCAGTTCCAATTGAAGACACCATGGAGGAGCTTAAAAAACTGGTAAATGAAGGAAAGATAAGACATATTGGGCTGTCGGACGCTAGTGGCGACACTATAAAGAGAGCACACGCAGTTCATCCTATTACTGCTTTGCAAATGGAGTATTCCTTATGGTCCCGTGACATTGAAGAAGAAATAATTCCACTCTGCAGAGAACTAGGGATTGGAATAGTAGCATATAGCCCCCTTGGCTGCGGCTTTTTTGCAGGAAAGTCGGTGGTAGAGGCTTTGCCTAGTCAGAGCGTACTGAATATGCATCCCAGGTTCACTGGAGTAAATCTGGAAAAGAACAAGATAATCTATGAACGAATCAATGTATTGGCTTCAAAGCATGCATGCACTCCTTCTCAATTAGCATTAGCATGGCTTCTGCATCAGGGAAATGACATAATCCCTATCCCCGGGACTACTAAAGTGAAGAACTTTGAAAACAACATTGGATCTCTGATTTTAAAGCTTACAGAAGAAGATTTGAAAGAAATTTCTGATGCAGTTCCTGTTGCAGAAATCGCTGGGAATCTAGAAAATCACAGTTTGTCGCAATATAGTTGGAAGTTTGCAACTACGCCGCCAAAGTAA

Protein sequence

>MsG0180005552.01.T01

MDKTEMQVPRVKLGSHGLEVSRLGFGCGGLSGIFNAPLSHEDGCSIIKEVFNRGVTFFDTSDLYGDNHDNEIMVGKALKELPREKVQLATKFGVTLSDGVFRVKGTPEYVRECCEASLKRLDVNYIDLYYQHRVDTSVPIEDTMEELKKLVNEGKIRHIGLSDASGDTIKRAHAVHPITALQMEYSLWSRDIEEEIIPLCRELGIGIVAYSPLGCGFFAGKSVVEALPSQSVLNMHPRFTGVNLEKNKIIYERINVLASKHACTPSQLALAWLLHQGNDIIPIPGTTKVKNFENNIGSLILKLTEEDLKEISDAVPVAEIAGNLENHSLSQYSWKFATTPPK*