Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005574.01.T01 | XP_013469781.1 | 96.757 | 185 | 6 | 0 | 2 | 186 | 1 | 185 | 5.93E-129 | 370 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005574.01.T01 | P05332 | 33.929 | 168 | 102 | 3 | 24 | 185 | 8 | 172 | 8.08E-24 | 95.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005574.01.T01 | A0A072VP71 | 96.757 | 185 | 6 | 0 | 2 | 186 | 1 | 185 | 2.83e-129 | 370 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180005574.01.T01 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005574.01.T01 | MTR_1g103150 | 96.757 | 185 | 6 | 0 | 2 | 186 | 1 | 185 | 7.19e-133 | 370 |
| MsG0180005574.01.T01 | MTR_1g103110 | 84.865 | 185 | 28 | 0 | 2 | 186 | 1 | 185 | 3.83e-114 | 323 |
| MsG0180005574.01.T01 | MTR_5g006470 | 62.766 | 188 | 68 | 1 | 2 | 189 | 1 | 186 | 4.08e-81 | 239 |
| MsG0180005574.01.T01 | MTR_5g006450 | 62.903 | 186 | 65 | 2 | 2 | 185 | 1 | 184 | 1.29e-80 | 238 |
| MsG0180005574.01.T01 | MTR_5g006520 | 59.686 | 191 | 73 | 2 | 1 | 189 | 1718 | 1906 | 4.30e-72 | 236 |
| MsG0180005574.01.T01 | MTR_5g006460 | 58.763 | 194 | 71 | 6 | 2 | 189 | 1 | 191 | 1.70e-68 | 207 |
| MsG0180005574.01.T01 | MTR_5g006510 | 52.356 | 191 | 86 | 3 | 2 | 189 | 1 | 189 | 8.58e-67 | 203 |
| MsG0180005574.01.T01 | MTR_7g114410 | 48.235 | 170 | 83 | 2 | 20 | 187 | 4 | 170 | 1.24e-48 | 156 |
| MsG0180005574.01.T01 | MTR_3g463610 | 44.186 | 172 | 88 | 2 | 20 | 186 | 3 | 171 | 7.16e-46 | 150 |
| MsG0180005574.01.T01 | MTR_7g114410 | 48.718 | 156 | 75 | 2 | 20 | 173 | 4 | 156 | 4.49e-44 | 144 |
| MsG0180005574.01.T01 | MTR_7g024765 | 42.683 | 164 | 91 | 3 | 23 | 186 | 7 | 167 | 4.73e-38 | 129 |
| MsG0180005574.01.T01 | MTR_7g024760 | 42.424 | 165 | 92 | 3 | 23 | 187 | 7 | 168 | 1.23e-37 | 128 |
| MsG0180005574.01.T01 | MTR_7g024780 | 39.881 | 168 | 99 | 2 | 18 | 185 | 2 | 167 | 2.26e-37 | 127 |
| MsG0180005574.01.T01 | MTR_1g103320 | 57.282 | 103 | 43 | 1 | 54 | 155 | 11 | 113 | 1.26e-33 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005574.01.T01 | AT2G32020 | 52.727 | 165 | 73 | 3 | 23 | 186 | 18 | 178 | 1.32e-54 | 172 |
| MsG0180005574.01.T01 | AT2G32030 | 45.989 | 187 | 96 | 3 | 1 | 186 | 1 | 183 | 2.27e-54 | 171 |
| MsG0180005574.01.T01 | AT3G22560 | 41.420 | 169 | 92 | 2 | 19 | 183 | 2 | 167 | 4.32e-36 | 124 |
Find 47 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAAAATATCTGTTCATTA+AGG | 0.174922 | 1:-93517704 | MsG0180005574.01.T01:CDS |
| TGTGGTTGAAGCTGCATTTA+AGG | 0.195666 | 1:-93517827 | MsG0180005574.01.T01:CDS |
| TTCTTTGAGCTCCATGTATT+GGG | 0.213171 | 1:-93517881 | MsG0180005574.01.T01:CDS |
| CTCTTCTTTAGCATCTGATT+TGG | 0.248198 | 1:+93518176 | None:intergenic |
| ATTCTTTGAGCTCCATGTAT+TGG | 0.293943 | 1:-93517882 | MsG0180005574.01.T01:CDS |
| GCCTCAAGCCTCTCCAAGTA+TGG | 0.329009 | 1:+93517799 | None:intergenic |
| GAGATTTGCTTGCTATGTTT+TGG | 0.329625 | 1:+93518004 | None:intergenic |
| CCTTATACAAACAAAGAAAA+AGG | 0.343403 | 1:-93518039 | MsG0180005574.01.T01:CDS |
| TGCCTTCGCGATAGAGCAAT+TGG | 0.346097 | 1:-93517964 | MsG0180005574.01.T01:CDS |
| GGGCAAAGGGATTGCCACTA+TGG | 0.352044 | 1:-93517860 | MsG0180005574.01.T01:CDS |
| TCAAGATCAGAAAGACTAAT+TGG | 0.352059 | 1:+93518117 | None:intergenic |
| TTTAGCATCTGATTTGGAAT+AGG | 0.367598 | 1:+93518182 | None:intergenic |
| AATTGGGTGTACCGAGTTCC+AGG | 0.375481 | 1:-93517947 | MsG0180005574.01.T01:CDS |
| AGAAAATATCTGTTCATTAA+GGG | 0.384110 | 1:-93517703 | MsG0180005574.01.T01:CDS |
| ATTTAAGGAGTTTCCATACT+TGG | 0.408342 | 1:-93517812 | MsG0180005574.01.T01:CDS |
| ACATAGCAAGCAAATCTCTA+TGG | 0.412043 | 1:-93517999 | MsG0180005574.01.T01:CDS |
| CAAAGGGTGTTGGAGAAGGC+TGG | 0.437360 | 1:-93517748 | MsG0180005574.01.T01:CDS |
| TACCGAGTTCCAGGTGTGTT+CGG | 0.452367 | 1:-93517938 | MsG0180005574.01.T01:CDS |
| TAATTGGTCGAAGAGAGATT+TGG | 0.457252 | 1:+93518133 | None:intergenic |
| CGGGACAAATCTGCTCAACT+TGG | 0.459831 | 1:-93517907 | MsG0180005574.01.T01:CDS |
| GGCATCTATCCGAACACACC+TGG | 0.486937 | 1:+93517929 | None:intergenic |
| AGCTCCATGTATTGGGGCAA+AGG | 0.494926 | 1:-93517874 | MsG0180005574.01.T01:CDS |
| AATCCCTTTGCCCCAATACA+TGG | 0.496567 | 1:+93517870 | None:intergenic |
| AGGTGTGTTCGGATAGATGC+CGG | 0.506000 | 1:-93517927 | MsG0180005574.01.T01:CDS |
| TTCTCAAAGGGTGTTGGAGA+AGG | 0.511828 | 1:-93517752 | MsG0180005574.01.T01:CDS |
| GCCTTCGCGATAGAGCAATT+GGG | 0.515394 | 1:-93517963 | MsG0180005574.01.T01:CDS |
| GCTCCATGTATTGGGGCAAA+GGG | 0.517436 | 1:-93517873 | MsG0180005574.01.T01:CDS |
| ATCTTGATGATGTTATGTTG+TGG | 0.524636 | 1:-93518101 | MsG0180005574.01.T01:CDS |
| AGGTTCCCAAGTGCAATACT+TGG | 0.527166 | 1:+93518059 | None:intergenic |
| CAAGTTGAGCAGATTTGTCC+CGG | 0.527345 | 1:+93517908 | None:intergenic |
| GAAAATATCTGTTCATTAAG+GGG | 0.536787 | 1:-93517702 | MsG0180005574.01.T01:CDS |
| TGTGGCTTCTCAAAGGGTGT+TGG | 0.546349 | 1:-93517758 | MsG0180005574.01.T01:CDS |
| TCCATACTTGGAGAGGCTTG+AGG | 0.557426 | 1:-93517800 | MsG0180005574.01.T01:CDS |
| CACATGTTTCACAACCATAG+TGG | 0.561077 | 1:+93517846 | None:intergenic |
| GCTTGAGGCTCGAGTTGATG+TGG | 0.569120 | 1:-93517785 | MsG0180005574.01.T01:CDS |
| TGGAAAATGTGGCTTCTCAA+AGG | 0.572207 | 1:-93517765 | MsG0180005574.01.T01:CDS |
| AGGAGTTTCCATACTTGGAG+AGG | 0.574247 | 1:-93517807 | MsG0180005574.01.T01:CDS |
| ATCCGAACACACCTGGAACT+CGG | 0.577234 | 1:+93517936 | None:intergenic |
| TCTTTGAGCTCCATGTATTG+GGG | 0.587210 | 1:-93517880 | MsG0180005574.01.T01:CDS |
| GGAAAATGTGGCTTCTCAAA+GGG | 0.588500 | 1:-93517764 | MsG0180005574.01.T01:CDS |
| AAGTGGCCAAGTATTGCACT+TGG | 0.595225 | 1:-93518065 | MsG0180005574.01.T01:CDS |
| GGTGTGTTCGGATAGATGCC+GGG | 0.599178 | 1:-93517926 | MsG0180005574.01.T01:CDS |
| GTGGACTAGTGATGCAAAAG+TGG | 0.603948 | 1:-93518082 | MsG0180005574.01.T01:CDS |
| ACCCAATTGCTCTATCGCGA+AGG | 0.616201 | 1:+93517962 | None:intergenic |
| TCGAGTTGATGTGGAAAATG+TGG | 0.669414 | 1:-93517776 | MsG0180005574.01.T01:CDS |
| AGTGGCCAAGTATTGCACTT+GGG | 0.670287 | 1:-93518064 | MsG0180005574.01.T01:CDS |
| CACTATGGTTGTGAAACATG+TGG | 0.680456 | 1:-93517845 | MsG0180005574.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAATATCTGTTCATTAA+GGG | - | Chr1:93518152-93518171 | MsG0180005574.01.T01:CDS | 20.0% |
| ! | CAGAAAATATCTGTTCATTA+AGG | - | Chr1:93518151-93518170 | MsG0180005574.01.T01:CDS | 25.0% |
| ! | CCTTATACAAACAAAGAAAA+AGG | - | Chr1:93517816-93517835 | MsG0180005574.01.T01:CDS | 25.0% |
| ! | GAAAATATCTGTTCATTAAG+GGG | - | Chr1:93518153-93518172 | MsG0180005574.01.T01:CDS | 25.0% |
| !!! | CCTTTTTCTTTGTTTGTATA+AGG | + | Chr1:93517819-93517838 | None:intergenic | 25.0% |
| ATTTAAGGAGTTTCCATACT+TGG | - | Chr1:93518043-93518062 | MsG0180005574.01.T01:CDS | 30.0% | |
| TCAAGATCAGAAAGACTAAT+TGG | + | Chr1:93517741-93517760 | None:intergenic | 30.0% | |
| !! | ATCTTGATGATGTTATGTTG+TGG | - | Chr1:93517754-93517773 | MsG0180005574.01.T01:CDS | 30.0% |
| !! | TTTAGCATCTGATTTGGAAT+AGG | + | Chr1:93517676-93517695 | None:intergenic | 30.0% |
| ACATAGCAAGCAAATCTCTA+TGG | - | Chr1:93517856-93517875 | MsG0180005574.01.T01:CDS | 35.0% | |
| ATTCTTTGAGCTCCATGTAT+TGG | - | Chr1:93517973-93517992 | MsG0180005574.01.T01:CDS | 35.0% | |
| CTCTTCTTTAGCATCTGATT+TGG | + | Chr1:93517682-93517701 | None:intergenic | 35.0% | |
| TAATTGGTCGAAGAGAGATT+TGG | + | Chr1:93517725-93517744 | None:intergenic | 35.0% | |
| TTCTTTGAGCTCCATGTATT+GGG | - | Chr1:93517974-93517993 | MsG0180005574.01.T01:CDS | 35.0% | |
| !!! | GAGATTTGCTTGCTATGTTT+TGG | + | Chr1:93517854-93517873 | None:intergenic | 35.0% |
| CACATGTTTCACAACCATAG+TGG | + | Chr1:93518012-93518031 | None:intergenic | 40.0% | |
| CACTATGGTTGTGAAACATG+TGG | - | Chr1:93518010-93518029 | MsG0180005574.01.T01:CDS | 40.0% | |
| GGAAAATGTGGCTTCTCAAA+GGG | - | Chr1:93518091-93518110 | MsG0180005574.01.T01:CDS | 40.0% | |
| TCTTTGAGCTCCATGTATTG+GGG | - | Chr1:93517975-93517994 | MsG0180005574.01.T01:CDS | 40.0% | |
| TGGAAAATGTGGCTTCTCAA+AGG | - | Chr1:93518090-93518109 | MsG0180005574.01.T01:CDS | 40.0% | |
| TGTGGTTGAAGCTGCATTTA+AGG | - | Chr1:93518028-93518047 | MsG0180005574.01.T01:CDS | 40.0% | |
| !! | TCGAGTTGATGTGGAAAATG+TGG | - | Chr1:93518079-93518098 | MsG0180005574.01.T01:CDS | 40.0% |
| AATCCCTTTGCCCCAATACA+TGG | + | Chr1:93517988-93518007 | None:intergenic | 45.0% | |
| AGGAGTTTCCATACTTGGAG+AGG | - | Chr1:93518048-93518067 | MsG0180005574.01.T01:CDS | 45.0% | |
| AGGTTCCCAAGTGCAATACT+TGG | + | Chr1:93517799-93517818 | None:intergenic | 45.0% | |
| ! | AAGTGGCCAAGTATTGCACT+TGG | - | Chr1:93517790-93517809 | MsG0180005574.01.T01:CDS | 45.0% |
| ! | AGTGGCCAAGTATTGCACTT+GGG | - | Chr1:93517791-93517810 | MsG0180005574.01.T01:CDS | 45.0% |
| ! | CAAGTTGAGCAGATTTGTCC+CGG | + | Chr1:93517950-93517969 | None:intergenic | 45.0% |
| !! | GTGGACTAGTGATGCAAAAG+TGG | - | Chr1:93517773-93517792 | MsG0180005574.01.T01:CDS | 45.0% |
| !! | TTCTCAAAGGGTGTTGGAGA+AGG | - | Chr1:93518103-93518122 | MsG0180005574.01.T01:CDS | 45.0% |
| !!! | AAGGCTGGTTTTGAAAGGGA+AGG | - | Chr1:93518122-93518141 | MsG0180005574.01.T01:CDS | 45.0% |
| !!! | GGAGAAGGCTGGTTTTGAAA+GGG | - | Chr1:93518118-93518137 | MsG0180005574.01.T01:CDS | 45.0% |
| !!! | TGGAGAAGGCTGGTTTTGAA+AGG | - | Chr1:93518117-93518136 | MsG0180005574.01.T01:CDS | 45.0% |
| AATTGGGTGTACCGAGTTCC+AGG | - | Chr1:93517908-93517927 | MsG0180005574.01.T01:CDS | 50.0% | |
| ACCCAATTGCTCTATCGCGA+AGG | + | Chr1:93517896-93517915 | None:intergenic | 50.0% | |
| AGCTCCATGTATTGGGGCAA+AGG | - | Chr1:93517981-93518000 | MsG0180005574.01.T01:CDS | 50.0% | |
| AGGTGTGTTCGGATAGATGC+CGG | - | Chr1:93517928-93517947 | MsG0180005574.01.T01:CDS | 50.0% | |
| ATCCGAACACACCTGGAACT+CGG | + | Chr1:93517922-93517941 | None:intergenic | 50.0% | |
| CGGGACAAATCTGCTCAACT+TGG | - | Chr1:93517948-93517967 | MsG0180005574.01.T01:CDS | 50.0% | |
| GCCTTCGCGATAGAGCAATT+GGG | - | Chr1:93517892-93517911 | MsG0180005574.01.T01:CDS | 50.0% | |
| GCTCCATGTATTGGGGCAAA+GGG | - | Chr1:93517982-93518001 | MsG0180005574.01.T01:CDS | 50.0% | |
| TACCGAGTTCCAGGTGTGTT+CGG | - | Chr1:93517917-93517936 | MsG0180005574.01.T01:CDS | 50.0% | |
| TCCATACTTGGAGAGGCTTG+AGG | - | Chr1:93518055-93518074 | MsG0180005574.01.T01:CDS | 50.0% | |
| TGCCTTCGCGATAGAGCAAT+TGG | - | Chr1:93517891-93517910 | MsG0180005574.01.T01:CDS | 50.0% | |
| ! | TGTGGCTTCTCAAAGGGTGT+TGG | - | Chr1:93518097-93518116 | MsG0180005574.01.T01:CDS | 50.0% |
| GCCTCAAGCCTCTCCAAGTA+TGG | + | Chr1:93518059-93518078 | None:intergenic | 55.0% | |
| GGCATCTATCCGAACACACC+TGG | + | Chr1:93517929-93517948 | None:intergenic | 55.0% | |
| GGGCAAAGGGATTGCCACTA+TGG | - | Chr1:93517995-93518014 | MsG0180005574.01.T01:CDS | 55.0% | |
| GGTGTGTTCGGATAGATGCC+GGG | - | Chr1:93517929-93517948 | MsG0180005574.01.T01:CDS | 55.0% | |
| !! | CAAAGGGTGTTGGAGAAGGC+TGG | - | Chr1:93518107-93518126 | MsG0180005574.01.T01:CDS | 55.0% |
| !! | GCTTGAGGCTCGAGTTGATG+TGG | - | Chr1:93518070-93518089 | MsG0180005574.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 93517654 | 93518223 | 93517654 | ID=MsG0180005574.01;Name=MsG0180005574.01 |
| Chr1 | mRNA | 93517654 | 93518223 | 93517654 | ID=MsG0180005574.01.T01;Parent=MsG0180005574.01;Name=MsG0180005574.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|189 |
| Chr1 | exon | 93517654 | 93518223 | 93517654 | ID=MsG0180005574.01.T01:exon:15290;Parent=MsG0180005574.01.T01 |
| Chr1 | CDS | 93517654 | 93518223 | 93517654 | ID=MsG0180005574.01.T01:cds;Parent=MsG0180005574.01.T01 |
| Gene Sequence |
| Protein sequence |
