Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024156.01.T01 | XP_003610742.1 | 88.083 | 193 | 21 | 1 | 1 | 193 | 1 | 191 | 4.29E-119 | 346 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024156.01.T01 | P05332 | 32.386 | 176 | 107 | 4 | 19 | 187 | 2 | 172 | 6.80E-21 | 88.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024156.01.T01 | G7K3P7 | 88.083 | 193 | 21 | 1 | 1 | 193 | 1 | 191 | 2.05e-119 | 346 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580024156.01.T01 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0580024156.01 | MsG0280009368.01 | PPI |
| MsG0780036978.01 | MsG0580024156.01 | PPI |
| MsG0580024154.01 | MsG0580024156.01 | PPI |
| MsG0780036979.01 | MsG0580024156.01 | PPI |
| MsG0180005574.01 | MsG0580024156.01 | PPI |
| MsG0580024156.01 | MsG0580024153.01 | PPI |
| MsG0580024152.01 | MsG0580024156.01 | PPI |
| MsG0280009366.01 | MsG0580024156.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024156.01.T01 | MTR_5g006510 | 88.083 | 193 | 21 | 1 | 1 | 193 | 1 | 191 | 5.19e-123 | 346 |
| MsG0580024156.01.T01 | MTR_5g006460 | 80.829 | 193 | 29 | 3 | 9 | 193 | 1 | 193 | 2.23e-108 | 309 |
| MsG0580024156.01.T01 | MTR_5g006470 | 72.626 | 179 | 44 | 3 | 17 | 193 | 13 | 188 | 1.41e-89 | 261 |
| MsG0580024156.01.T01 | MTR_5g006450 | 70.681 | 191 | 49 | 3 | 9 | 193 | 1 | 190 | 4.88e-89 | 260 |
| MsG0580024156.01.T01 | MTR_5g006520 | 68.508 | 181 | 54 | 2 | 15 | 193 | 1729 | 1908 | 4.15e-77 | 251 |
| MsG0580024156.01.T01 | MTR_1g103110 | 61.538 | 182 | 63 | 4 | 16 | 193 | 12 | 190 | 3.27e-74 | 222 |
| MsG0580024156.01.T01 | MTR_1g103150 | 59.659 | 176 | 66 | 3 | 20 | 193 | 18 | 190 | 1.49e-71 | 216 |
| MsG0580024156.01.T01 | MTR_7g114410 | 44.186 | 172 | 90 | 3 | 21 | 190 | 4 | 171 | 4.79e-43 | 142 |
| MsG0580024156.01.T01 | MTR_7g024760 | 47.024 | 168 | 83 | 4 | 24 | 190 | 7 | 169 | 2.37e-42 | 141 |
| MsG0580024156.01.T01 | MTR_7g024780 | 45.349 | 172 | 89 | 3 | 20 | 190 | 3 | 170 | 3.17e-42 | 140 |
| MsG0580024156.01.T01 | MTR_3g463610 | 42.105 | 171 | 98 | 1 | 21 | 190 | 3 | 173 | 4.23e-41 | 139 |
| MsG0580024156.01.T01 | MTR_7g024765 | 44.970 | 169 | 85 | 4 | 24 | 190 | 7 | 169 | 5.00e-39 | 132 |
| MsG0580024156.01.T01 | MTR_7g114410 | 43.312 | 157 | 83 | 3 | 21 | 175 | 4 | 156 | 2.24e-37 | 127 |
| MsG0580024156.01.T01 | MTR_1g103320 | 50.000 | 82 | 39 | 1 | 53 | 134 | 10 | 89 | 1.36e-17 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024156.01.T01 | AT2G32030 | 48.705 | 193 | 86 | 6 | 1 | 190 | 3 | 185 | 3.15e-54 | 171 |
| MsG0580024156.01.T01 | AT2G32020 | 51.765 | 170 | 72 | 5 | 24 | 190 | 18 | 180 | 3.90e-52 | 166 |
| MsG0580024156.01.T01 | AT3G22560 | 38.857 | 175 | 99 | 4 | 20 | 190 | 2 | 172 | 5.65e-34 | 119 |
Find 36 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAAGTATGTATTCTTTAA+AGG | 0.161729 | 5:-1400570 | MsG0580024156.01.T01:CDS |
| TTGTTGATGTGGATAATTTA+AGG | 0.203228 | 5:-1400641 | MsG0580024156.01.T01:CDS |
| CAAATCAATGATCTCTTCTT+TGG | 0.204931 | 5:+1401028 | None:intergenic |
| GTTGATGCCTTGGTCTTTGC+TGG | 0.262298 | 5:+1400905 | None:intergenic |
| GCTTGAAGCCTTTCCAAGTA+TGG | 0.276638 | 5:+1400666 | None:intergenic |
| TGCCATAACGATCATGCTAT+TGG | 0.288303 | 5:-1400837 | MsG0580024156.01.T01:CDS |
| GCAGAACTTGGGTATGTTCT+AGG | 0.307665 | 5:-1400762 | MsG0580024156.01.T01:CDS |
| ATGTTCTAGGCTCTAAATAC+TGG | 0.313478 | 5:-1400749 | MsG0580024156.01.T01:CDS |
| CCTTGGTCTTTGCTGGTGTA+AGG | 0.327596 | 5:+1400912 | None:intergenic |
| GAAAAGATCGGAGTGTGATT+TGG | 0.386444 | 5:+1401003 | None:intergenic |
| ACCTCGATGATCTCATGGTT+TGG | 0.401354 | 5:-1400971 | MsG0580024156.01.T01:CDS |
| GGCTCTAAATACTGGGGTAA+AGG | 0.409375 | 5:-1400741 | MsG0580024156.01.T01:CDS |
| ATTCAGTGAATTTCCATACT+TGG | 0.413337 | 5:-1400679 | MsG0580024156.01.T01:CDS |
| GCCATAACGATCATGCTATT+GGG | 0.423146 | 5:-1400836 | MsG0580024156.01.T01:CDS |
| AGGATCACATGTGCAGAACT+TGG | 0.424567 | 5:-1400774 | MsG0580024156.01.T01:CDS |
| TTCTGACCTCGATGATCTCA+TGG | 0.433346 | 5:-1400976 | MsG0580024156.01.T01:CDS |
| GCTCTAAATACTGGGGTAAA+GGG | 0.442106 | 5:-1400740 | MsG0580024156.01.T01:CDS |
| TGAGTTCACTTGATGATAAG+AGG | 0.442621 | 5:-1400797 | MsG0580024156.01.T01:CDS |
| CAAAGAGTAGTTGAGAAAGT+TGG | 0.450786 | 5:-1400615 | MsG0580024156.01.T01:CDS |
| ACCCAATAGCATGATCGTTA+TGG | 0.457877 | 5:+1400835 | None:intergenic |
| CTACTGATCTTAAACTAATG+AGG | 0.474389 | 5:-1400512 | MsG0580024156.01.T01:CDS |
| GTAAGGTTCACCCCAGAACT+TGG | 0.480302 | 5:+1400929 | None:intergenic |
| TAAACTAATGAGGATGTTCA+AGG | 0.483858 | 5:-1400502 | MsG0580024156.01.T01:CDS |
| GTGAATTTCCATACTTGGAA+AGG | 0.490341 | 5:-1400674 | MsG0580024156.01.T01:CDS |
| TGCTGTGAAACAAATGGTTA+AGG | 0.500098 | 5:-1400706 | MsG0580024156.01.T01:CDS |
| TGTTCTAGGCTCTAAATACT+GGG | 0.526941 | 5:-1400748 | MsG0580024156.01.T01:CDS |
| CTTATCATCAAGTGAACTCA+AGG | 0.556721 | 5:+1400800 | None:intergenic |
| GCTTCAAGCTCTTGTTGATG+TGG | 0.560091 | 5:-1400652 | MsG0580024156.01.T01:CDS |
| GGATCACATGTGCAGAACTT+GGG | 0.577727 | 5:-1400773 | MsG0580024156.01.T01:CDS |
| GGTCAGAAAGATGAAAAGAT+CGG | 0.592412 | 5:+1400991 | None:intergenic |
| CATTTGTTTCACAGCACATG+TGG | 0.625218 | 5:+1400713 | None:intergenic |
| CACATGTGCTGTGAAACAAA+TGG | 0.647600 | 5:-1400712 | MsG0580024156.01.T01:CDS |
| GTTCACTTGATGATAAGAGG+AGG | 0.664638 | 5:-1400794 | MsG0580024156.01.T01:CDS |
| CCTTACACCAGCAAAGACCA+AGG | 0.673207 | 5:-1400912 | MsG0580024156.01.T01:CDS |
| GTTCTAGGCTCTAAATACTG+GGG | 0.712321 | 5:-1400747 | MsG0580024156.01.T01:CDS |
| GCCAAACCATGAGATCATCG+AGG | 0.781144 | 5:+1400970 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATCAACTTCATTAAAAATA+TGG | - | Chr5:1400658-1400677 | MsG0580024156.01.T01:CDS | 20.0% |
| ! | TTGTTGATGTGGATAATTTA+AGG | - | Chr5:1400906-1400925 | MsG0580024156.01.T01:CDS | 25.0% |
| !! | AGGAAGTATGTATTCTTTAA+AGG | - | Chr5:1400977-1400996 | MsG0580024156.01.T01:CDS | 25.0% |
| !!! | AAAGTTGGTTTTCAAAAAGA+GGG | - | Chr5:1400947-1400966 | MsG0580024156.01.T01:CDS | 25.0% |
| ATTCAGTGAATTTCCATACT+TGG | - | Chr5:1400868-1400887 | MsG0580024156.01.T01:CDS | 30.0% | |
| CAAATCAATGATCTCTTCTT+TGG | + | Chr5:1400522-1400541 | None:intergenic | 30.0% | |
| CTACTGATCTTAAACTAATG+AGG | - | Chr5:1401035-1401054 | MsG0580024156.01.T01:CDS | 30.0% | |
| TAAACTAATGAGGATGTTCA+AGG | - | Chr5:1401045-1401064 | MsG0580024156.01.T01:CDS | 30.0% | |
| TCATTAAAAATATGGCAAGC+AGG | - | Chr5:1400666-1400685 | MsG0580024156.01.T01:CDS | 30.0% | |
| !!! | GAAAGTTGGTTTTCAAAAAG+AGG | - | Chr5:1400946-1400965 | MsG0580024156.01.T01:CDS | 30.0% |
| !!! | TTTTAATGAAGTTGATGCCT+TGG | + | Chr5:1400655-1400674 | None:intergenic | 30.0% |
| CAAAGAGTAGTTGAGAAAGT+TGG | - | Chr5:1400932-1400951 | MsG0580024156.01.T01:CDS | 35.0% | |
| CTTATCATCAAGTGAACTCA+AGG | + | Chr5:1400750-1400769 | None:intergenic | 35.0% | |
| GGTCAGAAAGATGAAAAGAT+CGG | + | Chr5:1400559-1400578 | None:intergenic | 35.0% | |
| GTGAATTTCCATACTTGGAA+AGG | - | Chr5:1400873-1400892 | MsG0580024156.01.T01:CDS | 35.0% | |
| TGCTGTGAAACAAATGGTTA+AGG | - | Chr5:1400841-1400860 | MsG0580024156.01.T01:CDS | 35.0% | |
| TTCAAAAAGAGGGAGTTCTT+AGG | - | Chr5:1400957-1400976 | MsG0580024156.01.T01:CDS | 35.0% | |
| TTGGCATACTGATGAAAAAG+TGG | - | Chr5:1400595-1400614 | MsG0580024156.01.T01:CDS | 35.0% | |
| ! | TGAGTTCACTTGATGATAAG+AGG | - | Chr5:1400750-1400769 | MsG0580024156.01.T01:CDS | 35.0% |
| !! | ATGTTCTAGGCTCTAAATAC+TGG | - | Chr5:1400798-1400817 | MsG0580024156.01.T01:CDS | 35.0% |
| !! | TGTTCTAGGCTCTAAATACT+GGG | - | Chr5:1400799-1400818 | MsG0580024156.01.T01:CDS | 35.0% |
| ACCCAATAGCATGATCGTTA+TGG | + | Chr5:1400715-1400734 | None:intergenic | 40.0% | |
| ATGAAAAAGTGGCCAAGTTC+TGG | - | Chr5:1400606-1400625 | MsG0580024156.01.T01:CDS | 40.0% | |
| CACATGTGCTGTGAAACAAA+TGG | - | Chr5:1400835-1400854 | MsG0580024156.01.T01:CDS | 40.0% | |
| CATTTGTTTCACAGCACATG+TGG | + | Chr5:1400837-1400856 | None:intergenic | 40.0% | |
| GAAAAGATCGGAGTGTGATT+TGG | + | Chr5:1400547-1400566 | None:intergenic | 40.0% | |
| GCCATAACGATCATGCTATT+GGG | - | Chr5:1400711-1400730 | MsG0580024156.01.T01:CDS | 40.0% | |
| TGAAAAAGTGGCCAAGTTCT+GGG | - | Chr5:1400607-1400626 | MsG0580024156.01.T01:CDS | 40.0% | |
| TGCCATAACGATCATGCTAT+TGG | - | Chr5:1400710-1400729 | MsG0580024156.01.T01:CDS | 40.0% | |
| ! | GCTCTAAATACTGGGGTAAA+GGG | - | Chr5:1400807-1400826 | MsG0580024156.01.T01:CDS | 40.0% |
| ! | GTTCACTTGATGATAAGAGG+AGG | - | Chr5:1400753-1400772 | MsG0580024156.01.T01:CDS | 40.0% |
| !! | GTTCTAGGCTCTAAATACTG+GGG | - | Chr5:1400800-1400819 | MsG0580024156.01.T01:CDS | 40.0% |
| ACCTCGATGATCTCATGGTT+TGG | - | Chr5:1400576-1400595 | MsG0580024156.01.T01:CDS | 45.0% | |
| AGGATCACATGTGCAGAACT+TGG | - | Chr5:1400773-1400792 | MsG0580024156.01.T01:CDS | 45.0% | |
| GAAAAAGTGGCCAAGTTCTG+GGG | - | Chr5:1400608-1400627 | MsG0580024156.01.T01:CDS | 45.0% | |
| GCAGAACTTGGGTATGTTCT+AGG | - | Chr5:1400785-1400804 | MsG0580024156.01.T01:CDS | 45.0% | |
| GCTTCAAGCTCTTGTTGATG+TGG | - | Chr5:1400895-1400914 | MsG0580024156.01.T01:CDS | 45.0% | |
| GCTTGAAGCCTTTCCAAGTA+TGG | + | Chr5:1400884-1400903 | None:intergenic | 45.0% | |
| GGATCACATGTGCAGAACTT+GGG | - | Chr5:1400774-1400793 | MsG0580024156.01.T01:CDS | 45.0% | |
| TTCTGACCTCGATGATCTCA+TGG | - | Chr5:1400571-1400590 | MsG0580024156.01.T01:CDS | 45.0% | |
| !! | GGCTCTAAATACTGGGGTAA+AGG | - | Chr5:1400806-1400825 | MsG0580024156.01.T01:CDS | 45.0% |
| CCTTACACCAGCAAAGACCA+AGG | - | Chr5:1400635-1400654 | MsG0580024156.01.T01:CDS | 50.0% | |
| GCCAAACCATGAGATCATCG+AGG | + | Chr5:1400580-1400599 | None:intergenic | 50.0% | |
| GTAAGGTTCACCCCAGAACT+TGG | + | Chr5:1400621-1400640 | None:intergenic | 50.0% | |
| ! | CCTTGGTCTTTGCTGGTGTA+AGG | + | Chr5:1400638-1400657 | None:intergenic | 50.0% |
| ! | GTTGATGCCTTGGTCTTTGC+TGG | + | Chr5:1400645-1400664 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 1400473 | 1401096 | 1400473 | ID=MsG0580024156.01;Name=MsG0580024156.01 |
| Chr5 | mRNA | 1400473 | 1401096 | 1400473 | ID=MsG0580024156.01.T01;Parent=MsG0580024156.01;Name=MsG0580024156.01.T01;_AED=0.51;_eAED=0.51;_QI=0|-1|0|1|-1|1|1|0|207 |
| Chr5 | exon | 1400473 | 1401096 | 1400473 | ID=MsG0580024156.01.T01:exon:458;Parent=MsG0580024156.01.T01 |
| Chr5 | CDS | 1400473 | 1401096 | 1400473 | ID=MsG0580024156.01.T01:cds;Parent=MsG0580024156.01.T01 |
| Gene Sequence |
| Protein sequence |
