Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009368.01.T01 | XP_003610737.1 | 76.282 | 156 | 9 | 1 | 1 | 128 | 1 | 156 | 2.12E-74 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009368.01.T01 | G7K3P2 | 76.282 | 156 | 9 | 1 | 1 | 128 | 1 | 156 | 1.01e-74 | 229 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0280009366.01 | MsG0280009368.01 | PPI |
| MsG0580024156.01 | MsG0280009368.01 | PPI |
| MsG0780036978.01 | MsG0280009368.01 | PPI |
| MsG0580024152.01 | MsG0280009368.01 | PPI |
| MsG0280009368.01 | MsG0580024153.01 | PPI |
| MsG0580024154.01 | MsG0280009368.01 | PPI |
| MsG0180005574.01 | MsG0280009368.01 | PPI |
| MsG0780036979.01 | MsG0280009368.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009368.01.T01 | MTR_5g006450 | 76.282 | 156 | 9 | 1 | 1 | 128 | 1 | 156 | 2.57e-78 | 229 |
| MsG0280009368.01.T01 | MTR_5g006470 | 64.286 | 154 | 29 | 1 | 1 | 128 | 1 | 154 | 2.80e-61 | 186 |
| MsG0280009368.01.T01 | MTR_5g006520 | 62.821 | 156 | 30 | 1 | 1 | 128 | 1719 | 1874 | 2.55e-58 | 194 |
| MsG0280009368.01.T01 | MTR_5g006460 | 58.642 | 162 | 32 | 6 | 1 | 129 | 1 | 160 | 1.72e-48 | 154 |
| MsG0280009368.01.T01 | MTR_5g006510 | 56.962 | 158 | 33 | 4 | 1 | 129 | 7 | 158 | 2.53e-48 | 154 |
| MsG0280009368.01.T01 | MTR_1g103110 | 53.205 | 156 | 45 | 3 | 1 | 128 | 1 | 156 | 1.69e-46 | 149 |
| MsG0280009368.01.T01 | MTR_1g103150 | 50.641 | 156 | 49 | 2 | 1 | 128 | 1 | 156 | 1.92e-45 | 146 |
| MsG0280009368.01.T01 | MTR_7g114410 | 30.435 | 138 | 71 | 1 | 16 | 128 | 3 | 140 | 1.36e-19 | 79.7 |
| MsG0280009368.01.T01 | MTR_7g114410 | 30.435 | 138 | 71 | 1 | 16 | 128 | 3 | 140 | 2.08e-19 | 79.7 |
| MsG0280009368.01.T01 | MTR_7g024760 | 32.857 | 140 | 68 | 3 | 20 | 135 | 7 | 144 | 5.55e-17 | 73.6 |
| MsG0280009368.01.T01 | MTR_3g463610 | 26.950 | 141 | 75 | 1 | 17 | 129 | 3 | 143 | 2.05e-16 | 72.8 |
| MsG0280009368.01.T01 | MTR_7g024765 | 30.714 | 140 | 71 | 3 | 20 | 135 | 7 | 144 | 3.92e-16 | 71.2 |
| MsG0280009368.01.T01 | MTR_7g024780 | 28.873 | 142 | 75 | 2 | 17 | 133 | 4 | 144 | 6.92e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009368.01.T01 | AT2G32030 | 40.000 | 150 | 59 | 3 | 3 | 129 | 14 | 155 | 1.75e-31 | 111 |
| MsG0280009368.01.T01 | AT2G32020 | 43.182 | 132 | 52 | 1 | 21 | 129 | 19 | 150 | 1.91e-30 | 108 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTAGAACTCCCTCTTAT+TGG | 0.154314 | 2:-51000163 | None:intergenic |
| CATCAAGGTCAGAAAGATTA+AGG | 0.227074 | 2:-50999767 | None:intergenic |
| CTCTTCTTTGTCACGTGGTT+TGG | 0.270188 | 2:-50999712 | None:intergenic |
| ATGTTCTAGGCTCTAAATAT+TGG | 0.292142 | 2:+51000004 | MsG0280009368.01.T01:intron |
| TAAGGGGTCGGAGTGTGATT+TGG | 0.307480 | 2:-50999749 | None:intergenic |
| TGTTCTAGGCTCTAAATATT+GGG | 0.316974 | 2:+51000005 | MsG0280009368.01.T01:intron |
| AAGGGGTCGGAGTGTGATTT+GGG | 0.320072 | 2:-50999748 | None:intergenic |
| ATGTGGGGTCTCAAGAGTTT+TGG | 0.348201 | 2:+51000127 | MsG0280009368.01.T01:CDS |
| GCTCTAAATATTGGGGTAAA+GGG | 0.356917 | 2:+51000013 | MsG0280009368.01.T01:CDS |
| TCCAATAAGAGGGAGTTCTA+AGG | 0.374852 | 2:+51000162 | MsG0280009368.01.T01:CDS |
| ATATAGCTACTAAGTTTCTA+TGG | 0.387531 | 2:+50999883 | MsG0280009368.01.T01:CDS |
| GAAAGCTGGTTTCCAATAAG+AGG | 0.410914 | 2:+51000151 | MsG0280009368.01.T01:CDS |
| GGCTCTAAATATTGGGGTAA+AGG | 0.422886 | 2:+51000012 | MsG0280009368.01.T01:intron |
| ATTCTGTGAGTTGTCTTACT+TGG | 0.446468 | 2:+51000074 | MsG0280009368.01.T01:CDS |
| GTTGATGCCATCATCCTTGC+TGG | 0.459050 | 2:-50999850 | None:intergenic |
| AGTGGCCAAGTTCTGCTCTT+GGG | 0.462852 | 2:+50999818 | MsG0280009368.01.T01:CDS |
| AAGTGGCCAAGTTCTGCTCT+TGG | 0.474319 | 2:+50999817 | MsG0280009368.01.T01:CDS |
| GTGAGTTGTCTTACTTGGAA+AGG | 0.487957 | 2:+51000079 | MsG0280009368.01.T01:CDS |
| GCTTGAAGCTTTAGTTGATG+TGG | 0.488968 | 2:+51000101 | MsG0280009368.01.T01:CDS |
| TTCTGACCTTGATGATCTCA+TGG | 0.490514 | 2:+50999776 | MsG0280009368.01.T01:CDS |
| ATCAAGGTCAGAAAGATTAA+GGG | 0.492658 | 2:-50999766 | None:intergenic |
| ACCTTGATGATCTCATGGTA+TGG | 0.538119 | 2:+50999781 | MsG0280009368.01.T01:CDS |
| TTTAGTTGATGTGGAAAATG+TGG | 0.542907 | 2:+51000110 | MsG0280009368.01.T01:CDS |
| TGTTGTGAAACAAGTGGTTA+AGG | 0.545474 | 2:+51000047 | MsG0280009368.01.T01:CDS |
| CTCTACGCCAGCAAGGATGA+TGG | 0.563263 | 2:+50999843 | MsG0280009368.01.T01:CDS |
| CTCTAAATATTGGGGTAAAG+GGG | 0.564900 | 2:+51000014 | MsG0280009368.01.T01:CDS |
| GAGTTCCCAAGAGCAGAACT+TGG | 0.571214 | 2:-50999823 | None:intergenic |
| ATTCTCTCTTCTTTGTCACG+TGG | 0.580393 | 2:-50999717 | None:intergenic |
| GGTCAGAAAGATTAAGGGGT+CGG | 0.585362 | 2:-50999761 | None:intergenic |
| CACTTGTTTCACAACACACG+TGG | 0.596860 | 2:-51000040 | None:intergenic |
| TTAGTTGATGTGGAAAATGT+GGG | 0.609276 | 2:+51000111 | MsG0280009368.01.T01:CDS |
| GTTCTAGGCTCTAAATATTG+GGG | 0.619040 | 2:+51000006 | MsG0280009368.01.T01:intron |
| TCCATACCATGAGATCATCA+AGG | 0.632686 | 2:-50999782 | None:intergenic |
| AAAGCTGGTTTCCAATAAGA+GGG | 0.633115 | 2:+51000152 | MsG0280009368.01.T01:CDS |
| TCAAGGTCAGAAAGATTAAG+GGG | 0.633672 | 2:-50999765 | None:intergenic |
| TTGGGAACTCTACGCCAGCA+AGG | 0.634354 | 2:+50999836 | MsG0280009368.01.T01:CDS |
| TAGTTGATGTGGAAAATGTG+GGG | 0.695063 | 2:+51000112 | MsG0280009368.01.T01:CDS |
| CACGTGTGTTGTGAAACAAG+TGG | 0.736637 | 2:+51000041 | MsG0280009368.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATAGCTACTAAGTTTCTA+TGG | + | Chr2:50999883-50999902 | MsG0280009368.01.T01:CDS | 25.0% |
| ATCAAGGTCAGAAAGATTAA+GGG | - | Chr2:50999769-50999788 | None:intergenic | 30.0% | |
| ATTGTTCCTTGATTTATCAC+AGG | - | Chr2:50999969-50999988 | None:intergenic | 30.0% | |
| ! | TTAGTTGATGTGGAAAATGT+GGG | + | Chr2:51000111-51000130 | MsG0280009368.01.T01:CDS | 30.0% |
| ! | TTTAGTTGATGTGGAAAATG+TGG | + | Chr2:51000110-51000129 | MsG0280009368.01.T01:CDS | 30.0% |
| !! | ATGTTCTAGGCTCTAAATAT+TGG | + | Chr2:51000004-51000023 | MsG0280009368.01.T01:intron | 30.0% |
| !! | TGTTCTAGGCTCTAAATATT+GGG | + | Chr2:51000005-51000024 | MsG0280009368.01.T01:intron | 30.0% |
| AGTTGACCTGTGATAAATCA+AGG | + | Chr2:50999960-50999979 | MsG0280009368.01.T01:intron | 35.0% | |
| ATGGACAACTGATGAAAAAG+TGG | + | Chr2:50999800-50999819 | MsG0280009368.01.T01:CDS | 35.0% | |
| ATTCTGTGAGTTGTCTTACT+TGG | + | Chr2:51000074-51000093 | MsG0280009368.01.T01:CDS | 35.0% | |
| ATTTATCACAGGTCAACTTG+AGG | - | Chr2:50999958-50999977 | None:intergenic | 35.0% | |
| CATCAAGGTCAGAAAGATTA+AGG | - | Chr2:50999770-50999789 | None:intergenic | 35.0% | |
| CTCTAAATATTGGGGTAAAG+GGG | + | Chr2:51000014-51000033 | MsG0280009368.01.T01:CDS | 35.0% | |
| TCAAGGTCAGAAAGATTAAG+GGG | - | Chr2:50999768-50999787 | None:intergenic | 35.0% | |
| TGTTGTGAAACAAGTGGTTA+AGG | + | Chr2:51000047-51000066 | MsG0280009368.01.T01:CDS | 35.0% | |
| ! | AAAGCTGGTTTCCAATAAGA+GGG | + | Chr2:51000152-51000171 | MsG0280009368.01.T01:CDS | 35.0% |
| ! | GCTCTAAATATTGGGGTAAA+GGG | + | Chr2:51000013-51000032 | MsG0280009368.01.T01:CDS | 35.0% |
| ! | TAGTTGATGTGGAAAATGTG+GGG | + | Chr2:51000112-51000131 | MsG0280009368.01.T01:CDS | 35.0% |
| !! | GTTCTAGGCTCTAAATATTG+GGG | + | Chr2:51000006-51000025 | MsG0280009368.01.T01:intron | 35.0% |
| AAGGAACAATGTGCAGAACT+TGG | + | Chr2:50999979-50999998 | MsG0280009368.01.T01:intron | 40.0% | |
| ATTCTCTCTTCTTTGTCACG+TGG | - | Chr2:50999720-50999739 | None:intergenic | 40.0% | |
| GCATCAATGATCGTGCAATT+GGG | + | Chr2:50999919-50999938 | MsG0280009368.01.T01:intron | 40.0% | |
| GTGAGTTGTCTTACTTGGAA+AGG | + | Chr2:51000079-51000098 | MsG0280009368.01.T01:CDS | 40.0% | |
| TCCATACCATGAGATCATCA+AGG | - | Chr2:50999785-50999804 | None:intergenic | 40.0% | |
| TGCATCAATGATCGTGCAAT+TGG | + | Chr2:50999918-50999937 | MsG0280009368.01.T01:intron | 40.0% | |
| ! | ACCTTGATGATCTCATGGTA+TGG | + | Chr2:50999781-50999800 | MsG0280009368.01.T01:CDS | 40.0% |
| ! | GAAAGCTGGTTTCCAATAAG+AGG | + | Chr2:51000151-51000170 | MsG0280009368.01.T01:CDS | 40.0% |
| ! | GCTTGAAGCTTTAGTTGATG+TGG | + | Chr2:51000101-51000120 | MsG0280009368.01.T01:CDS | 40.0% |
| ! | TTCTGACCTTGATGATCTCA+TGG | + | Chr2:50999776-50999795 | MsG0280009368.01.T01:CDS | 40.0% |
| !! | GGCTCTAAATATTGGGGTAA+AGG | + | Chr2:51000012-51000031 | MsG0280009368.01.T01:intron | 40.0% |
| !!! | TCAAGAGTTTTGGAGAAAGC+TGG | + | Chr2:51000137-51000156 | MsG0280009368.01.T01:CDS | 40.0% |
| CACGTGTGTTGTGAAACAAG+TGG | + | Chr2:51000041-51000060 | MsG0280009368.01.T01:CDS | 45.0% | |
| CACTTGTTTCACAACACACG+TGG | - | Chr2:51000043-51000062 | None:intergenic | 45.0% | |
| CTCTTCTTTGTCACGTGGTT+TGG | - | Chr2:50999715-50999734 | None:intergenic | 45.0% | |
| GCAGAACTTGGCTATGTTCT+AGG | + | Chr2:50999991-51000010 | MsG0280009368.01.T01:intron | 45.0% | |
| GGTCAGAAAGATTAAGGGGT+CGG | - | Chr2:50999764-50999783 | None:intergenic | 45.0% | |
| ! | ATGTGGGGTCTCAAGAGTTT+TGG | + | Chr2:51000127-51000146 | MsG0280009368.01.T01:CDS | 45.0% |
| AAGGGGTCGGAGTGTGATTT+GGG | - | Chr2:50999751-50999770 | None:intergenic | 50.0% | |
| AAGTGGCCAAGTTCTGCTCT+TGG | + | Chr2:50999817-50999836 | MsG0280009368.01.T01:CDS | 50.0% | |
| AGTGGCCAAGTTCTGCTCTT+GGG | + | Chr2:50999818-50999837 | MsG0280009368.01.T01:CDS | 50.0% | |
| GAGTTCCCAAGAGCAGAACT+TGG | - | Chr2:50999826-50999845 | None:intergenic | 50.0% | |
| GTTGATGCCATCATCCTTGC+TGG | - | Chr2:50999853-50999872 | None:intergenic | 50.0% | |
| TAAGGGGTCGGAGTGTGATT+TGG | - | Chr2:50999752-50999771 | None:intergenic | 50.0% | |
| CTCTACGCCAGCAAGGATGA+TGG | + | Chr2:50999843-50999862 | MsG0280009368.01.T01:CDS | 55.0% | |
| TTGGGAACTCTACGCCAGCA+AGG | + | Chr2:50999836-50999855 | MsG0280009368.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 50999690 | 51000182 | 50999690 | ID=MsG0280009368.01;Name=MsG0280009368.01 |
| Chr2 | mRNA | 50999690 | 51000182 | 50999690 | ID=MsG0280009368.01.T01;Parent=MsG0280009368.01;Name=MsG0280009368.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|136 |
| Chr2 | exon | 50999690 | 50999930 | 50999690 | ID=MsG0280009368.01.T01:exon:24023;Parent=MsG0280009368.01.T01 |
| Chr2 | exon | 51000013 | 51000182 | 51000013 | ID=MsG0280009368.01.T01:exon:24024;Parent=MsG0280009368.01.T01 |
| Chr2 | CDS | 50999690 | 50999930 | 50999690 | ID=MsG0280009368.01.T01:cds;Parent=MsG0280009368.01.T01 |
| Chr2 | CDS | 51000013 | 51000182 | 51000013 | ID=MsG0280009368.01.T01:cds;Parent=MsG0280009368.01.T01 |
| Gene Sequence |
| Protein sequence |
