AlfalfaGEDB | MsG0580024153.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024153.01

KEGG: -
GO: -
PFAM: PF13302

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024153.01.T01	AES93696.1	77.654	179	35	4	1	175	1	178	2.38E-87	264

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024153.01.T01	G7K3P3	77.654	179	35	4	1	175	1	178	1.14e-87	264

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0180005574.01	MsG0580024153.01	PPI
MsG0280009366.01	MsG0580024153.01	PPI
MsG0780036978.01	MsG0580024153.01	PPI
MsG0280009368.01	MsG0580024153.01	PPI
MsG0580024152.01	MsG0580024153.01	PPI
MsG0580024156.01	MsG0580024153.01	PPI
MsG0580024154.01	MsG0580024153.01	PPI
MsG0780036979.01	MsG0580024153.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024153.01.T01	MTR_5g006460	77.654	179	35	4	1	175	1	178	2.88e-91	264
MsG0580024153.01.T01	MTR_5g006510	71.023	176	46	2	1	176	7	177	1.21e-83	245
MsG0580024153.01.T01	MTR_5g006450	67.797	177	53	3	1	175	1	175	3.50e-75	223
MsG0580024153.01.T01	MTR_5g006470	66.477	176	55	3	1	175	1	173	1.01e-74	222
MsG0580024153.01.T01	MTR_5g006520	64.205	176	61	2	1	175	1719	1893	5.42e-67	221
MsG0580024153.01.T01	MTR_1g103110	58.580	169	62	5	1	165	1	165	1.34e-57	179
MsG0580024153.01.T01	MTR_1g103150	55.866	179	71	5	1	175	1	175	3.18e-57	178
MsG0580024153.01.T01	MTR_7g024780	38.217	157	93	2	16	172	3	155	1.95e-29	107
MsG0580024153.01.T01	MTR_7g114410	36.943	157	96	1	17	173	4	157	1.15e-28	104
MsG0580024153.01.T01	MTR_7g114410	37.584	149	90	1	17	165	4	149	7.44e-28	102
MsG0580024153.01.T01	MTR_7g024760	40.278	144	79	4	20	162	7	144	1.24e-27	102
MsG0580024153.01.T01	MTR_7g024765	37.662	154	89	4	20	172	7	154	2.68e-27	101
MsG0580024153.01.T01	MTR_3g463610	35.570	149	96	0	17	165	3	151	1.76e-24	95.5
MsG0580024153.01.T01	MTR_1g103320	51.220	82	38	1	50	131	10	89	6.48e-19	78.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024153.01.T01	AT2G32020	47.436	156	71	6	20	172	18	165	1.07e-35	123
MsG0580024153.01.T01	AT2G32030	47.742	155	70	6	21	172	24	170	1.60e-34	120
MsG0580024153.01.T01	AT3G22560	33.987	153	94	3	16	165	2	150	4.68e-21	85.5

Find 35 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGAAGTATGTATTCTTTAA+AGG	0.161729	5:-1390962	MsG0580024153.01.T01:CDS
ATGAAAAGGTAGCCAAGTTC+TGG	0.273400	5:-1391332	MsG0580024153.01.T01:CDS
CTCTTCATTGGCACATGGTT+TGG	0.276019	5:+1391434	None:intergenic
GCTTCAAGCCTTTCCAAGTA+TGG	0.276638	5:+1391057	None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	5:-1391140	MsG0580024153.01.T01:CDS
TGTTCTAGGCTCTAAATATT+GGG	0.316974	5:-1391139	MsG0580024153.01.T01:CDS
TTGTTGATGTGGATAATTTA+TGG	0.328127	5:-1391032	MsG0580024153.01.T01:CDS
TGCCATAATGATCATGCTAT+TGG	0.333891	5:-1391228	MsG0580024153.01.T01:CDS
ACCCAATAGCATGATCATTA+TGG	0.341789	5:+1391226	None:intergenic
CCTTCGTCTTTGCTAGTGTA+AGG	0.376096	5:+1391303	None:intergenic
CAAATCAATGACCTCTTCAT+TGG	0.385362	5:+1391422	None:intergenic
GGCTCTAAATATTGGGGTAA+AGG	0.406739	5:-1391132	MsG0580024153.01.T01:CDS
ATTCAGTGAATTTCCATACT+TGG	0.413337	5:-1391070	MsG0580024153.01.T01:CDS
CACGTTTGCTGTGAAACAAA+TGG	0.422199	5:-1391103	MsG0580024153.01.T01:CDS
GCTCTAAATATTGGGGTAAA+GGG	0.428389	5:-1391131	MsG0580024153.01.T01:CDS
TTCTGACCTTGATGATGTAA+TGG	0.440976	5:-1391370	MsG0580024153.01.T01:CDS
CAAAGAGTAGTTGAGAAAGT+TGG	0.450786	5:-1391006	MsG0580024153.01.T01:CDS
GAAGATGTCGGAGTGTGATT+TGG	0.453912	5:+1391397	None:intergenic
TGCTGTGAAACAAATGGTTA+AGG	0.473285	5:-1391097	MsG0580024153.01.T01:CDS
GCCATAATGATCATGCTATT+GGG	0.478546	5:-1391227	MsG0580024153.01.T01:CDS
TGAAAAGGTAGCCAAGTTCT+GGG	0.482152	5:-1391331	MsG0580024153.01.T01:CDS
GTGAATTTCCATACTTGGAA+AGG	0.490341	5:-1391065	MsG0580024153.01.T01:CDS
GCTTGAAGCTCTTGTTGATG+TGG	0.580383	5:-1391043	MsG0580024153.01.T01:CDS
CAAACCATGTGCCAATGAAG+AGG	0.589377	5:-1391433	MsG0580024153.01.T01:CDS
GTGGCAGCAGACTGATGAAA+AGG	0.594266	5:-1391346	MsG0580024153.01.T01:CDS
GCCACACCATTACATCATCA+AGG	0.594693	5:+1391364	None:intergenic
ATGACCTCTTCATTGGCACA+TGG	0.616227	5:+1391429	None:intergenic
AAAAGGTAGCCAAGTTCTGG+GGG	0.620200	5:-1391329	MsG0580024153.01.T01:CDS
GAAAAGGTAGCCAAGTTCTG+GGG	0.624494	5:-1391330	MsG0580024153.01.T01:CDS
GTTCTAGGCTCTAAATATTG+GGG	0.628624	5:-1391138	MsG0580024153.01.T01:CDS
GGTCAGAAAGATGAAGATGT+CGG	0.630507	5:+1391385	None:intergenic
CATTTGTTTCACAGCAAACG+TGG	0.659491	5:+1391104	None:intergenic
ACCTTGATGATGTAATGGTG+TGG	0.697032	5:-1391365	MsG0580024153.01.T01:CDS
CCTTACACTAGCAAAGACGA+AGG	0.715657	5:-1391303	MsG0580024153.01.T01:CDS
AAAGGTAGCCAAGTTCTGGG+GGG	0.779565	5:-1391328	MsG0580024153.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAATCAAATGTGCAAAAAT+TGG	-	Chr5:1391236-1391255	MsG0580024153.01.T01:CDS	25.0%
!	GAATCAAATGTGCAAAAATT+GGG	-	Chr5:1391237-1391256	MsG0580024153.01.T01:CDS	25.0%
!	TTGTTGATGTGGATAATTTA+TGG	-	Chr5:1391369-1391388	MsG0580024153.01.T01:CDS	25.0%
!!	AGGAAGTATGTATTCTTTAA+AGG	-	Chr5:1391439-1391458	MsG0580024153.01.T01:CDS	25.0%
	ATTCAGTGAATTTCCATACT+TGG	-	Chr5:1391331-1391350	MsG0580024153.01.T01:CDS	30.0%
	TTTCAAAAAGAGGAGTTCTT+AGG	-	Chr5:1391419-1391438	MsG0580024153.01.T01:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	Chr5:1391261-1391280	MsG0580024153.01.T01:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	Chr5:1391262-1391281	MsG0580024153.01.T01:CDS	30.0%
!!!	GAAAGTTGGTTTTCAAAAAG+AGG	-	Chr5:1391409-1391428	MsG0580024153.01.T01:CDS	30.0%
	ACCCAATAGCATGATCATTA+TGG	+	Chr5:1391178-1391197	None:intergenic	35.0%
	CAAAGAGTAGTTGAGAAAGT+TGG	-	Chr5:1391395-1391414	MsG0580024153.01.T01:CDS	35.0%
	CAAATCAATGACCTCTTCAT+TGG	+	Chr5:1390982-1391001	None:intergenic	35.0%
	GCAAAAATTGGGTATGTTCT+AGG	-	Chr5:1391248-1391267	MsG0580024153.01.T01:CDS	35.0%
	GCCATAATGATCATGCTATT+GGG	-	Chr5:1391174-1391193	MsG0580024153.01.T01:CDS	35.0%
	GTGAATTTCCATACTTGGAA+AGG	-	Chr5:1391336-1391355	MsG0580024153.01.T01:CDS	35.0%
	TGCCATAATGATCATGCTAT+TGG	-	Chr5:1391173-1391192	MsG0580024153.01.T01:CDS	35.0%
	TGCTGTGAAACAAATGGTTA+AGG	-	Chr5:1391304-1391323	MsG0580024153.01.T01:CDS	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	Chr5:1391270-1391289	MsG0580024153.01.T01:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	Chr5:1391263-1391282	MsG0580024153.01.T01:CDS	35.0%
!!	TTCTGACCTTGATGATGTAA+TGG	-	Chr5:1391031-1391050	MsG0580024153.01.T01:CDS	35.0%
	ATGAAAAGGTAGCCAAGTTC+TGG	-	Chr5:1391069-1391088	MsG0580024153.01.T01:CDS	40.0%
	CATTTGTTTCACAGCAAACG+TGG	+	Chr5:1391300-1391319	None:intergenic	40.0%
	GGTCAGAAAGATGAAGATGT+CGG	+	Chr5:1391019-1391038	None:intergenic	40.0%
	TGAAAAGGTAGCCAAGTTCT+GGG	-	Chr5:1391070-1391089	MsG0580024153.01.T01:CDS	40.0%
!!	ACCTTGATGATGTAATGGTG+TGG	-	Chr5:1391036-1391055	MsG0580024153.01.T01:CDS	40.0%
!!	CACGTTTGCTGTGAAACAAA+TGG	-	Chr5:1391298-1391317	MsG0580024153.01.T01:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	Chr5:1391269-1391288	MsG0580024153.01.T01:CDS	40.0%
	ATGACCTCTTCATTGGCACA+TGG	+	Chr5:1390975-1390994	None:intergenic	45.0%
	CAAACCATGTGCCAATGAAG+AGG	-	Chr5:1390968-1390987	MsG0580024153.01.T01:CDS	45.0%
	CCTTACACTAGCAAAGACGA+AGG	-	Chr5:1391098-1391117	MsG0580024153.01.T01:CDS	45.0%
	CCTTCGTCTTTGCTAGTGTA+AGG	+	Chr5:1391101-1391120	None:intergenic	45.0%
	CTCTTCATTGGCACATGGTT+TGG	+	Chr5:1390970-1390989	None:intergenic	45.0%
	GAAAAGGTAGCCAAGTTCTG+GGG	-	Chr5:1391071-1391090	MsG0580024153.01.T01:CDS	45.0%
	GAAGATGTCGGAGTGTGATT+TGG	+	Chr5:1391007-1391026	None:intergenic	45.0%
	GCCACACCATTACATCATCA+AGG	+	Chr5:1391040-1391059	None:intergenic	45.0%
	GCTTCAAGCCTTTCCAAGTA+TGG	+	Chr5:1391347-1391366	None:intergenic	45.0%
	GCTTGAAGCTCTTGTTGATG+TGG	-	Chr5:1391358-1391377	MsG0580024153.01.T01:CDS	45.0%
!	AAAAGGTAGCCAAGTTCTGG+GGG	-	Chr5:1391072-1391091	MsG0580024153.01.T01:CDS	45.0%
	GTGGCAGCAGACTGATGAAA+AGG	-	Chr5:1391055-1391074	MsG0580024153.01.T01:CDS	50.0%
!!	AAAGGTAGCCAAGTTCTGGG+GGG	-	Chr5:1391073-1391092	MsG0580024153.01.T01:CDS	50.0%
	GTAAGGTTCCCCCCAGAACT+TGG	+	Chr5:1391084-1391103	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	1390945	1391478	1390945	ID=MsG0580024153.01;Name=MsG0580024153.01
Chr5	mRNA	1390945	1391478	1390945	ID=MsG0580024153.01.T01;Parent=MsG0580024153.01;Name=MsG0580024153.01.T01;_AED=0.48;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|177
Chr5	exon	1390945	1391478	1390945	ID=MsG0580024153.01.T01:exon:453;Parent=MsG0580024153.01.T01
Chr5	CDS	1390945	1391478	1390945	ID=MsG0580024153.01.T01:cds;Parent=MsG0580024153.01.T01

Gene Sequence

>MsG0580024153.01.T01

ATGGAAGAGACTTCTATTTTCTCCAAACCATGTGCCAATGAAGAGGTCATTGATTTGAACCAAATCACACTCCGACATCTTCATCTTTCTGACCTTGATGATGTAATGGTGTGGCAGCAGACTGATGAAAAGGTAGCCAAGTTCTGGGGGGAACCTTACACTAGCAAAGACGAAGGCATCAACTTCATTGAAAATATAGAAAGTGATTATCTACGTTGCGAAGCAATATGCCATAATGATCATGCTATTGGGTGTATTAAGTTGTTCTCGAGTTCACGTCGTGATCTAAGCAGAATCAAATGTGCAAAAATTGGGTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGATTACCACGTTTGCTGTGAAACAAATGGTTAAGGTTGCATTCAGTGAATTTCCATACTTGGAAAGGCTTGAAGCTCTTGTTGATGTGGATAATTTATGGTCGCAAAGAGTAGTTGAGAAAGTTGGTTTTCAAAAAGAGGAGTTCTTAGGAAGTATGTATTCTTTAAAGGAAAAAGCAGAGATATGA

Protein sequence

>MsG0580024153.01.T01

MEETSIFSKPCANEEVIDLNQITLRHLHLSDLDDVMVWQQTDEKVAKFWGEPYTSKDEGINFIENIESDYLRCEAICHNDHAIGCIKLFSSSRRDLSRIKCAKIGYVLGSKYWGKGITTFAVKQMVKVAFSEFPYLERLEALVDVDNLWSQRVVEKVGFQKEEFLGSMYSLKEKAEI*