Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009366.01.T01 | XP_003610737.1 | 93.548 | 155 | 9 | 1 | 1 | 154 | 36 | 190 | 6.67E-99 | 292 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009366.01.T01 | P05332 | 44.186 | 86 | 47 | 1 | 64 | 149 | 88 | 172 | 7.00E-14 | 68.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009366.01.T01 | G7K3P2 | 93.548 | 155 | 9 | 1 | 1 | 154 | 36 | 190 | 3.19e-99 | 292 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009366.01.T01 | MTR_5g006450 | 93.548 | 155 | 9 | 1 | 1 | 154 | 36 | 190 | 8.08e-103 | 292 |
| MsG0280009366.01.T01 | MTR_5g006470 | 78.710 | 155 | 30 | 2 | 1 | 154 | 36 | 188 | 2.42e-80 | 236 |
| MsG0280009366.01.T01 | MTR_5g006520 | 79.355 | 155 | 31 | 1 | 1 | 154 | 1754 | 1908 | 3.17e-78 | 252 |
| MsG0280009366.01.T01 | MTR_5g006520 | 52.632 | 76 | 11 | 3 | 62 | 136 | 1655 | 1706 | 2.17e-12 | 63.9 |
| MsG0280009366.01.T01 | MTR_5g006510 | 73.718 | 156 | 37 | 3 | 1 | 154 | 38 | 191 | 6.47e-76 | 224 |
| MsG0280009366.01.T01 | MTR_5g006460 | 73.585 | 159 | 35 | 5 | 1 | 154 | 37 | 193 | 1.00e-72 | 216 |
| MsG0280009366.01.T01 | MTR_1g103110 | 67.333 | 150 | 47 | 1 | 1 | 150 | 38 | 185 | 7.57e-69 | 206 |
| MsG0280009366.01.T01 | MTR_1g103150 | 63.333 | 150 | 53 | 1 | 1 | 150 | 38 | 185 | 4.25e-64 | 194 |
| MsG0280009366.01.T01 | MTR_3g463610 | 36.424 | 151 | 96 | 0 | 1 | 151 | 22 | 172 | 1.37e-33 | 118 |
| MsG0280009366.01.T01 | MTR_7g114410 | 38.667 | 150 | 89 | 1 | 1 | 150 | 23 | 169 | 4.28e-33 | 115 |
| MsG0280009366.01.T01 | MTR_7g114410 | 39.416 | 137 | 80 | 1 | 1 | 137 | 23 | 156 | 5.26e-31 | 109 |
| MsG0280009366.01.T01 | MTR_7g024760 | 38.000 | 150 | 88 | 3 | 1 | 150 | 23 | 167 | 5.68e-29 | 105 |
| MsG0280009366.01.T01 | MTR_7g024780 | 36.913 | 149 | 90 | 2 | 1 | 149 | 23 | 167 | 5.14e-28 | 102 |
| MsG0280009366.01.T01 | MTR_7g024765 | 35.333 | 150 | 92 | 3 | 1 | 150 | 23 | 167 | 3.96e-27 | 100 |
| MsG0280009366.01.T01 | MTR_1g103320 | 50.000 | 78 | 37 | 1 | 18 | 95 | 13 | 88 | 3.07e-17 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009366.01.T01 | AT2G32030 | 50.331 | 151 | 68 | 3 | 1 | 150 | 39 | 183 | 1.34e-48 | 155 |
| MsG0280009366.01.T01 | AT2G32020 | 49.007 | 151 | 70 | 2 | 1 | 150 | 34 | 178 | 1.88e-44 | 144 |
| MsG0280009366.01.T01 | AT3G22560 | 37.748 | 151 | 89 | 2 | 3 | 151 | 24 | 171 | 6.74e-29 | 104 |
Find 33 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGGGTCTCAAAGAGTTT+TGG | 0.282550 | 2:+50980766 | MsG0280009366.01.T01:CDS |
| ATGTTCTAGGCTATAAATAT+TGG | 0.289347 | 2:+50980642 | MsG0280009366.01.T01:CDS |
| TGTTCTAGGCTATAAATATT+GGG | 0.307390 | 2:+50980643 | MsG0280009366.01.T01:CDS |
| GCAGAACTTGGCTATGTTCT+AGG | 0.310077 | 2:+50980629 | MsG0280009366.01.T01:CDS |
| GCTATAAATATTGGGGTAAA+GGG | 0.313408 | 2:+50980651 | MsG0280009366.01.T01:CDS |
| CCAAACAATTGAATTATAGT+TGG | 0.393357 | 2:-50980886 | None:intergenic |
| ATATAGCTACCAAGTTTCTA+TGG | 0.402105 | 2:+50980519 | MsG0280009366.01.T01:CDS |
| GGCTATAAATATTGGGGTAA+AGG | 0.427948 | 2:+50980650 | MsG0280009366.01.T01:CDS |
| GTTGATGCCATCATCCTTGC+TGG | 0.429573 | 2:-50980486 | None:intergenic |
| ATTCTGTGAGTTGTCTTACT+TGG | 0.446468 | 2:+50980712 | MsG0280009366.01.T01:CDS |
| AGTGGCCAAGTTCTGCTCTT+GGG | 0.462852 | 2:+50980454 | MsG0280009366.01.T01:CDS |
| AAGTGGCCAAGTTCTGCTCT+TGG | 0.474319 | 2:+50980453 | MsG0280009366.01.T01:CDS |
| AGGAAATATTTGTTCATGAA+AGG | 0.480337 | 2:+50980821 | MsG0280009366.01.T01:CDS |
| AGGAACAAATGTGCAGAACT+TGG | 0.481600 | 2:+50980617 | MsG0280009366.01.T01:CDS |
| GTGAGTTGTCTTACTTGGAA+AGG | 0.487957 | 2:+50980717 | MsG0280009366.01.T01:CDS |
| GCTTGAAGCTTTAGTTGATG+TGG | 0.488968 | 2:+50980739 | MsG0280009366.01.T01:CDS |
| TAGTTGATGTGGAAAATGTT+GGG | 0.494202 | 2:+50980750 | MsG0280009366.01.T01:CDS |
| TGCTTTGCACCATAGAAACT+TGG | 0.496119 | 2:-50980528 | None:intergenic |
| TTGTTCCTTGATTTATCACC+AGG | 0.502130 | 2:-50980602 | None:intergenic |
| GTTCACCTGGTGATAAATCA+AGG | 0.507986 | 2:+50980597 | MsG0280009366.01.T01:CDS |
| TTAGTTGATGTGGAAAATGT+TGG | 0.523001 | 2:+50980749 | MsG0280009366.01.T01:CDS |
| ACCTTGATGATCTCATGGTA+TGG | 0.538119 | 2:+50980417 | None:intergenic |
| TCTCTATCCTCAAGTTCACC+TGG | 0.545696 | 2:+50980584 | MsG0280009366.01.T01:CDS |
| TGTTGTGAAACAAGAGGTTA+AGG | 0.553508 | 2:+50980685 | MsG0280009366.01.T01:CDS |
| CTCTACACCAGCAAGGATGA+TGG | 0.559987 | 2:+50980479 | MsG0280009366.01.T01:CDS |
| GAGTTCCCAAGAGCAGAACT+TGG | 0.571214 | 2:-50980459 | None:intergenic |
| CTATAAATATTGGGGTAAAG+GGG | 0.577289 | 2:+50980652 | MsG0280009366.01.T01:CDS |
| TTTATCACCAGGTGAACTTG+AGG | 0.579933 | 2:-50980591 | None:intergenic |
| GTTCTAGGCTATAAATATTG+GGG | 0.598552 | 2:+50980644 | MsG0280009366.01.T01:CDS |
| CTCTTGTTTCACAACACACG+TGG | 0.611497 | 2:-50980678 | None:intergenic |
| TTGGAGATCAGTAAGAACAC+TGG | 0.646655 | 2:-50980867 | None:intergenic |
| TTGGGAACTCTACACCAGCA+AGG | 0.679749 | 2:+50980472 | MsG0280009366.01.T01:CDS |
| CACGTGTGTTGTGAAACAAG+AGG | 0.741960 | 2:+50980679 | MsG0280009366.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGGAAATATTTGTTCATGAA+AGG | + | Chr2:50980821-50980840 | MsG0280009366.01.T01:CDS | 25.0% |
| !! | ATGTTCTAGGCTATAAATAT+TGG | + | Chr2:50980642-50980661 | MsG0280009366.01.T01:CDS | 25.0% |
| !! | TGTTCTAGGCTATAAATATT+GGG | + | Chr2:50980643-50980662 | MsG0280009366.01.T01:CDS | 25.0% |
| ATATAGCTACCAAGTTTCTA+TGG | + | Chr2:50980519-50980538 | MsG0280009366.01.T01:CDS | 30.0% | |
| CTATAAATATTGGGGTAAAG+GGG | + | Chr2:50980652-50980671 | MsG0280009366.01.T01:CDS | 30.0% | |
| GCTATAAATATTGGGGTAAA+GGG | + | Chr2:50980651-50980670 | MsG0280009366.01.T01:CDS | 30.0% | |
| ! | GTTCTAGGCTATAAATATTG+GGG | + | Chr2:50980644-50980663 | MsG0280009366.01.T01:CDS | 30.0% |
| ! | TAGTTGATGTGGAAAATGTT+GGG | + | Chr2:50980750-50980769 | MsG0280009366.01.T01:CDS | 30.0% |
| ! | TTAGTTGATGTGGAAAATGT+TGG | + | Chr2:50980749-50980768 | MsG0280009366.01.T01:CDS | 30.0% |
| ATGGACAACTGATGAAAAAG+TGG | + | Chr2:50980436-50980455 | MsG0280009366.01.T01:CDS | 35.0% | |
| ATTCTGTGAGTTGTCTTACT+TGG | + | Chr2:50980712-50980731 | MsG0280009366.01.T01:CDS | 35.0% | |
| GGCTATAAATATTGGGGTAA+AGG | + | Chr2:50980650-50980669 | MsG0280009366.01.T01:CDS | 35.0% | |
| TGTTGTGAAACAAGAGGTTA+AGG | + | Chr2:50980685-50980704 | MsG0280009366.01.T01:CDS | 35.0% | |
| TTGTTCCTTGATTTATCACC+AGG | - | Chr2:50980605-50980624 | None:intergenic | 35.0% | |
| ! | AAAGCTGGTTTCCAAAAAGA+GGG | + | Chr2:50980791-50980810 | MsG0280009366.01.T01:CDS | 35.0% |
| AGGAACAAATGTGCAGAACT+TGG | + | Chr2:50980617-50980636 | MsG0280009366.01.T01:CDS | 40.0% | |
| GTGAGTTGTCTTACTTGGAA+AGG | + | Chr2:50980717-50980736 | MsG0280009366.01.T01:CDS | 40.0% | |
| GTTCACCTGGTGATAAATCA+AGG | + | Chr2:50980597-50980616 | MsG0280009366.01.T01:CDS | 40.0% | |
| TCCAAAAAGAGGGAGTTCTA+AGG | + | Chr2:50980801-50980820 | MsG0280009366.01.T01:CDS | 40.0% | |
| TGCTTTGCACCATAGAAACT+TGG | - | Chr2:50980531-50980550 | None:intergenic | 40.0% | |
| TTGGAGATCAGTAAGAACAC+TGG | - | Chr2:50980870-50980889 | None:intergenic | 40.0% | |
| TTTATCACCAGGTGAACTTG+AGG | - | Chr2:50980594-50980613 | None:intergenic | 40.0% | |
| ! | GAAAGCTGGTTTCCAAAAAG+AGG | + | Chr2:50980790-50980809 | MsG0280009366.01.T01:CDS | 40.0% |
| ! | GCTTGAAGCTTTAGTTGATG+TGG | + | Chr2:50980739-50980758 | MsG0280009366.01.T01:CDS | 40.0% |
| ! | TCCTTAGAACTCCCTCTTTT+TGG | - | Chr2:50980805-50980824 | None:intergenic | 40.0% |
| ! | TGTTGGGTCTCAAAGAGTTT+TGG | + | Chr2:50980766-50980785 | MsG0280009366.01.T01:CDS | 40.0% |
| !!! | CAAAGAGTTTTGGAGAAAGC+TGG | + | Chr2:50980776-50980795 | MsG0280009366.01.T01:CDS | 40.0% |
| CACGTGTGTTGTGAAACAAG+AGG | + | Chr2:50980679-50980698 | MsG0280009366.01.T01:CDS | 45.0% | |
| GCAGAACTTGGCTATGTTCT+AGG | + | Chr2:50980629-50980648 | MsG0280009366.01.T01:CDS | 45.0% | |
| TCTCTATCCTCAAGTTCACC+TGG | + | Chr2:50980584-50980603 | MsG0280009366.01.T01:CDS | 45.0% | |
| ! | CTCTTGTTTCACAACACACG+TGG | - | Chr2:50980681-50980700 | None:intergenic | 45.0% |
| AAGTGGCCAAGTTCTGCTCT+TGG | + | Chr2:50980453-50980472 | MsG0280009366.01.T01:CDS | 50.0% | |
| AGTGGCCAAGTTCTGCTCTT+GGG | + | Chr2:50980454-50980473 | MsG0280009366.01.T01:CDS | 50.0% | |
| CTCTACACCAGCAAGGATGA+TGG | + | Chr2:50980479-50980498 | MsG0280009366.01.T01:CDS | 50.0% | |
| GAGTTCCCAAGAGCAGAACT+TGG | - | Chr2:50980462-50980481 | None:intergenic | 50.0% | |
| GTTGATGCCATCATCCTTGC+TGG | - | Chr2:50980489-50980508 | None:intergenic | 50.0% | |
| TTGGGAACTCTACACCAGCA+AGG | + | Chr2:50980472-50980491 | MsG0280009366.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 50980431 | 50980895 | 50980431 | ID=MsG0280009366.01;Name=MsG0280009366.01 |
| Chr2 | mRNA | 50980431 | 50980895 | 50980431 | ID=MsG0280009366.01.T01;Parent=MsG0280009366.01;Name=MsG0280009366.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|154 |
| Chr2 | exon | 50980431 | 50980895 | 50980431 | ID=MsG0280009366.01.T01:exon:8298;Parent=MsG0280009366.01.T01 |
| Chr2 | CDS | 50980431 | 50980895 | 50980431 | ID=MsG0280009366.01.T01:cds;Parent=MsG0280009366.01.T01 |
| Gene Sequence |
| Protein sequence |
