AlfalfaGEDB | MsG0580024154.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024154.01

KEGG: -
GO: -
PFAM: PF13302

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024154.01.T01	XP_039690105.1	92.553	188	14	0	1	188	1	188	1.05E-124	359

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024154.01.T01	P05332	33.143	175	106	4	15	182	2	172	1.81E-19	84.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024154.01.T01	G7K3P4	92.553	188	14	0	1	188	1	188	5.03e-125	359

TFs/TRs:

Gene ID	Type	Classification
MsG0580024154.01.T01	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048345.01	MsG0580024154.01	0.825417	4.741187e-54	4.318835e-51
MsG0080048348.01	MsG0580024154.01	0.804596	2.002476e-49	1.036685e-46
MsG0180000019.01	MsG0580024154.01	0.854967	8.646126e-62	1.965049e-58
MsG0180001050.01	MsG0580024154.01	0.855212	7.338443e-62	1.681403e-58
MsG0180001053.01	MsG0580024154.01	0.832765	7.842422e-56	8.851422e-53
MsG0180004181.01	MsG0580024154.01	0.818702	1.707698e-52	1.287567e-49
MsG0180004562.01	MsG0580024154.01	0.862846	3.793324e-64	1.126874e-60
MsG0180005357.01	MsG0580024154.01	0.801802	7.581915e-49	3.654349e-46
MsG0280011053.01	MsG0580024154.01	0.812534	4.038856e-51	2.574604e-48
MsG0280011152.01	MsG0580024154.01	0.838184	3.343627e-57	4.443517e-54
MsG0280011288.01	MsG0580024154.01	0.852882	3.447483e-61	7.309790e-58
MsG0280011349.01	MsG0580024154.01	0.830114	3.524181e-55	3.678380e-52
MsG0280011473.01	MsG0580024154.01	0.833399	5.453382e-56	6.273038e-53
MsG0380011613.01	MsG0580024154.01	0.829051	6.388899e-55	6.464347e-52
MsG0480021135.01	MsG0580024154.01	0.824926	6.193520e-54	5.564010e-51
MsG0480022125.01	MsG0580024154.01	0.800102	1.687260e-48	7.789519e-46
MsG0480022797.01	MsG0580024154.01	0.807091	5.986414e-50	3.304339e-47
MsG0580024154.01	MsG0580027073.01	0.839024	2.028396e-57	2.765796e-54
MsG0580024154.01	MsG0580027227.01	0.838704	2.455044e-57	3.315323e-54
MsG0580024154.01	MsG0580028999.01	0.831591	1.530918e-55	1.669025e-52
MsG0580024154.01	MsG0580029412.01	0.827214	1.769944e-54	1.697636e-51
MsG0580024154.01	MsG0580029930.01	0.829197	5.890713e-55	5.987143e-52
MsG0580024154.01	MsG0780039248.01	0.823350	1.452488e-53	1.247420e-50
MsG0580024154.01	MsG0780039334.01	0.822362	2.467264e-53	2.060777e-50
MsG0580024154.01	MsG0780039877.01	0.803006	4.283454e-49	2.128664e-46
MsG0580024154.01	MsG0780041646.01	0.809286	2.039282e-50	1.192521e-47
MsG0580024154.01	MsG0880043286.01	0.802667	5.031224e-49	2.478933e-46
MsG0580024154.01	MsG0880044305.01	0.800934	1.141583e-48	5.383078e-46
MsG0580024154.01	MsG0880044669.01	0.807356	5.260425e-50	2.923743e-47
MsG0580024154.01	MsG0880045275.01	0.810470	1.134510e-50	6.844533e-48
MsG0580024154.01	MsG0880045277.01	0.808081	3.689442e-50	2.089821e-47
MsG0580024154.01	MsG0880045441.01	0.809088	2.249465e-50	1.308440e-47
MsG0580024154.01	MsG0880047081.01	0.842123	3.133648e-58	4.704524e-55
MsG0580024154.01	MsG0880047486.01	0.810342	1.209158e-50	7.269964e-48
MsG0580024154.01	MsG0880047491.01	0.835059	2.091444e-56	2.528437e-53
MsG0380017020.01	MsG0580024154.01	0.808125	3.611435e-50	2.048006e-47
MsG0380017158.01	MsG0580024154.01	0.804751	1.858833e-49	9.662470e-47
MsG0280009394.01	MsG0580024154.01	0.812959	3.260742e-51	2.101781e-48

PPI

Gene1	Gene2	Type
MsG0580024152.01	MsG0580024154.01	PPI
MsG0580024154.01	MsG0580024156.01	PPI
MsG0580024154.01	MsG0180005574.01	PPI
MsG0580024154.01	MsG0280009366.01	PPI
MsG0580024154.01	MsG0780036979.01	PPI
MsG0580024154.01	MsG0280009368.01	PPI
MsG0580024154.01	MsG0580024153.01	PPI
MsG0580024154.01	MsG0780036978.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024154.01.T01	MTR_5g006470	92.553	188	14	0	1	188	1	188	1.28e-128	359
MsG0580024154.01.T01	MTR_5g006450	77.368	190	41	1	1	188	1	190	1.29e-101	291
MsG0580024154.01.T01	MTR_5g006460	76.410	195	37	6	1	188	1	193	7.37e-97	279
MsG0580024154.01.T01	MTR_5g006520	74.737	190	46	1	1	188	1719	1908	5.43e-93	296
MsG0580024154.01.T01	MTR_5g006510	73.184	179	43	3	13	188	15	191	1.28e-89	261
MsG0580024154.01.T01	MTR_1g103110	63.684	190	67	1	1	188	1	190	1.01e-84	248
MsG0580024154.01.T01	MTR_1g103150	62.105	190	70	1	1	188	1	190	6.65e-84	246
MsG0580024154.01.T01	MTR_7g114410	44.379	169	93	1	17	185	4	171	3.59e-45	147
MsG0580024154.01.T01	MTR_7g024780	42.353	170	96	2	16	185	3	170	9.39e-41	136
MsG0580024154.01.T01	MTR_7g114410	43.506	154	86	1	17	170	4	156	7.45e-40	133
MsG0580024154.01.T01	MTR_3g463610	41.860	172	96	2	17	185	3	173	2.69e-39	134
MsG0580024154.01.T01	MTR_7g024760	41.566	166	94	2	20	185	7	169	1.12e-38	130
MsG0580024154.01.T01	MTR_7g024765	41.566	166	94	2	20	185	7	169	9.55e-38	128
MsG0580024154.01.T01	MTR_1g103320	46.835	79	42	0	51	129	11	89	4.55e-19	79.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024154.01.T01	AT2G32020	55.689	167	69	3	20	185	18	180	2.92e-58	181
MsG0580024154.01.T01	AT2G32030	53.614	166	72	3	21	185	24	185	2.38e-56	176
MsG0580024154.01.T01	AT3G22560	38.372	172	103	2	16	185	2	172	5.43e-35	121

Find 36 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGAGTTCTTAGGAAGTATT+TGG	0.241791	5:-1393206	MsG0580024154.01.T01:CDS
ATAATCTAAGCTCTATATAT+TGG	0.258828	5:-1393375	MsG0580024154.01.T01:CDS
TCCTAAGAACTCCCTCCTTT+TGG	0.260104	5:+1393215	None:intergenic
ATCAAGGTCAGAAAGATTAA+AGG	0.290557	5:+1393609	None:intergenic
TAATCTAAGCTCTATATATT+GGG	0.291812	5:-1393374	MsG0580024154.01.T01:CDS
TGCTGTGAAACAAATTATTA+AGG	0.312252	5:-1393332	MsG0580024154.01.T01:CDS
AAAATGAAACTTATTAAAGT+TGG	0.351635	5:+1393128	None:intergenic
TCTCCAAGTATGAAAATTCA+TGG	0.373432	5:+1393302	None:intergenic
TCCAAAAGGAGGGAGTTCTT+AGG	0.394554	5:-1393216	MsG0580024154.01.T01:CDS
TAAAGGGTCGGAGTGTGATT+TGG	0.396804	5:+1393626	None:intergenic
TGCATTGATGATCGTGCTAT+TGG	0.407515	5:-1393457	MsG0580024154.01.T01:CDS
AGGAAACAATCTGCAGAACT+TGG	0.436712	5:-1393400	MsG0580024154.01.T01:CDS
TTCTGACCTTGATGATGTAA+TGG	0.440976	5:-1393599	MsG0580024154.01.T01:CDS
CCATCGTCTCTGCTGGTGTA+TGG	0.442715	5:+1393532	None:intergenic
GGTTGCCAAGTTCTGCTCTT+GGG	0.451345	5:-1393557	MsG0580024154.01.T01:CDS
GTTGATGCCATCGTCTCTGC+TGG	0.472064	5:+1393525	None:intergenic
TCAAGGTCAGAAAGATTAAA+GGG	0.477441	5:+1393610	None:intergenic
CTTGTCGATGTTGAAAATGT+TGG	0.484592	5:-1393268	MsG0580024154.01.T01:CDS
GCTCTATATATTGGGGCAAA+GGG	0.493310	5:-1393366	MsG0580024154.01.T01:CDS
AGGTTGCCAAGTTCTGCTCT+TGG	0.493839	5:-1393558	MsG0580024154.01.T01:CDS
CAAAGAGTGTTGGAGAAAGC+TGG	0.499760	5:-1393241	MsG0580024154.01.T01:CDS
AGCTCTATATATTGGGGCAA+AGG	0.501996	5:-1393367	MsG0580024154.01.T01:CDS
TTGTCGATGTTGAAAATGTT+GGG	0.503194	5:-1393267	MsG0580024154.01.T01:CDS
AGGAAGTATTTGGTCATGAA+AGG	0.520639	5:-1393196	MsG0580024154.01.T01:CDS
GGAGAAAGCTGGTTTCCAAA+AGG	0.547990	5:-1393230	MsG0580024154.01.T01:CDS
GAAAGCTGGTTTCCAAAAGG+AGG	0.562242	5:-1393227	MsG0580024154.01.T01:CDS
TGTTGGGTCACAAAGAGTGT+TGG	0.566749	5:-1393251	MsG0580024154.01.T01:CDS
TGGTTCCCAAGAGCAGAACT+TGG	0.574822	5:+1393552	None:intergenic
AATCTAAGCTCTATATATTG+GGG	0.593409	5:-1393373	MsG0580024154.01.T01:CDS
GCCACACCATTACATCATCA+AGG	0.594693	5:+1393593	None:intergenic
GGTCAGAAAGATTAAAGGGT+CGG	0.596865	5:+1393614	None:intergenic
TCACACCTGAAGATAAAAGC+AGG	0.598275	5:-1393420	MsG0580024154.01.T01:CDS
GGTGTGGCATACTGATGTAA+AGG	0.604925	5:-1393578	MsG0580024154.01.T01:CDS
CCATACACCAGCAGAGACGA+TGG	0.622747	5:-1393532	MsG0580024154.01.T01:CDS
AAAGCTGGTTTCCAAAAGGA+GGG	0.657724	5:-1393226	MsG0580024154.01.T01:CDS
ACCTTGATGATGTAATGGTG+TGG	0.686805	5:-1393594	MsG0580024154.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATCTAAGCTCTATATAT+TGG	-	Chr5:1393451-1393470	MsG0580024154.01.T01:CDS	20.0%
!!	TAATCTAAGCTCTATATATT+GGG	-	Chr5:1393452-1393471	MsG0580024154.01.T01:CDS	20.0%
!	AATCTAAGCTCTATATATTG+GGG	-	Chr5:1393453-1393472	MsG0580024154.01.T01:CDS	25.0%
!	TGCTGTGAAACAAATTATTA+AGG	-	Chr5:1393494-1393513	MsG0580024154.01.T01:CDS	25.0%
!!	ATTCCATGAATTTTCATACT+TGG	-	Chr5:1393521-1393540	MsG0580024154.01.T01:CDS	25.0%
	ATCAAGGTCAGAAAGATTAA+AGG	+	Chr5:1393220-1393239	None:intergenic	30.0%
	TCAAGGTCAGAAAGATTAAA+GGG	+	Chr5:1393219-1393238	None:intergenic	30.0%
	TCTCCAAGTATGAAAATTCA+TGG	+	Chr5:1393527-1393546	None:intergenic	30.0%
	TTGTCGATGTTGAAAATGTT+GGG	-	Chr5:1393559-1393578	MsG0580024154.01.T01:CDS	30.0%
!	ATGAATTTTCATACTTGGAG+AGG	-	Chr5:1393526-1393545	MsG0580024154.01.T01:CDS	30.0%
	CTTGTCGATGTTGAAAATGT+TGG	-	Chr5:1393558-1393577	MsG0580024154.01.T01:CDS	35.0%
!!	AGGAAGTATTTGGTCATGAA+AGG	-	Chr5:1393630-1393649	MsG0580024154.01.T01:CDS	35.0%
!!	TGTTTCCTGCTTTTATCTTC+AGG	+	Chr5:1393414-1393433	None:intergenic	35.0%
!!	TTCTGACCTTGATGATGTAA+TGG	-	Chr5:1393227-1393246	MsG0580024154.01.T01:CDS	35.0%
	AGCTCTATATATTGGGGCAA+AGG	-	Chr5:1393459-1393478	MsG0580024154.01.T01:CDS	40.0%
	AGGAAACAATCTGCAGAACT+TGG	-	Chr5:1393426-1393445	MsG0580024154.01.T01:CDS	40.0%
	GCTCTATATATTGGGGCAAA+GGG	-	Chr5:1393460-1393479	MsG0580024154.01.T01:CDS	40.0%
	GGTCAGAAAGATTAAAGGGT+CGG	+	Chr5:1393215-1393234	None:intergenic	40.0%
	TCACACCTGAAGATAAAAGC+AGG	-	Chr5:1393406-1393425	MsG0580024154.01.T01:CDS	40.0%
!	AAAGCTGGTTTCCAAAAGGA+GGG	-	Chr5:1393600-1393619	MsG0580024154.01.T01:CDS	40.0%
!	TGCATTGATGATCGTGCTAT+TGG	-	Chr5:1393369-1393388	MsG0580024154.01.T01:CDS	40.0%
!!	ACCTTGATGATGTAATGGTG+TGG	-	Chr5:1393232-1393251	MsG0580024154.01.T01:CDS	40.0%
!!	GGGAGTTCTTAGGAAGTATT+TGG	-	Chr5:1393620-1393639	MsG0580024154.01.T01:CDS	40.0%
	GCCACACCATTACATCATCA+AGG	+	Chr5:1393236-1393255	None:intergenic	45.0%
	GGTGTGGCATACTGATGTAA+AGG	-	Chr5:1393248-1393267	MsG0580024154.01.T01:CDS	45.0%
	TAAAGGGTCGGAGTGTGATT+TGG	+	Chr5:1393203-1393222	None:intergenic	45.0%
	TCCAAAAGGAGGGAGTTCTT+AGG	-	Chr5:1393610-1393629	MsG0580024154.01.T01:CDS	45.0%
!	GAAAGCTGGTTTCCAAAAGG+AGG	-	Chr5:1393599-1393618	MsG0580024154.01.T01:CDS	45.0%
!	GGAGAAAGCTGGTTTCCAAA+AGG	-	Chr5:1393596-1393615	MsG0580024154.01.T01:CDS	45.0%
!	TCCTAAGAACTCCCTCCTTT+TGG	+	Chr5:1393614-1393633	None:intergenic	45.0%
!	TGTTGGGTCACAAAGAGTGT+TGG	-	Chr5:1393575-1393594	MsG0580024154.01.T01:CDS	45.0%
!!	CAAAGAGTGTTGGAGAAAGC+TGG	-	Chr5:1393585-1393604	MsG0580024154.01.T01:CDS	45.0%
	AGGTTGCCAAGTTCTGCTCT+TGG	-	Chr5:1393268-1393287	MsG0580024154.01.T01:CDS	50.0%
	GGTTGCCAAGTTCTGCTCTT+GGG	-	Chr5:1393269-1393288	MsG0580024154.01.T01:CDS	50.0%
	TGGTTCCCAAGAGCAGAACT+TGG	+	Chr5:1393277-1393296	None:intergenic	50.0%
	CCATACACCAGCAGAGACGA+TGG	-	Chr5:1393294-1393313	MsG0580024154.01.T01:CDS	55.0%
	CCATCGTCTCTGCTGGTGTA+TGG	+	Chr5:1393297-1393316	None:intergenic	55.0%
	GTTGATGCCATCGTCTCTGC+TGG	+	Chr5:1393304-1393323	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	1393141	1393707	1393141	ID=MsG0580024154.01;Name=MsG0580024154.01
Chr5	mRNA	1393141	1393707	1393141	ID=MsG0580024154.01.T01;Parent=MsG0580024154.01;Name=MsG0580024154.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|188
Chr5	exon	1393141	1393707	1393141	ID=MsG0580024154.01.T01:exon:454;Parent=MsG0580024154.01.T01
Chr5	CDS	1393141	1393707	1393141	ID=MsG0580024154.01.T01:cds;Parent=MsG0580024154.01.T01

Gene Sequence

>MsG0580024154.01.T01

ATGGAGGAAACTTTTATTTCTTCAAAATCATGTGTCAAAGAAGAGATCATTGATTTGAACCAAATCACACTCCGACCCTTTAATCTTTCTGACCTTGATGATGTAATGGTGTGGCATACTGATGTAAAGGTTGCCAAGTTCTGCTCTTGGGAACCATACACCAGCAGAGACGATGGCATCAACTTCATTGAAAATATAGAAAGCAAGTTTCTTTGTTGCAAAGCAATATGCATTGATGATCGTGCTATTGGTTGTATTCTTTTGTTCACACCTGAAGATAAAAGCAGGAAACAATCTGCAGAACTTGGCTATAATCTAAGCTCTATATATTGGGGCAAAGGGATTGTCACGTGTGCTGTGAAACAAATTATTAAGGTTGCATTCCATGAATTTTCATACTTGGAGAGGCTTGAAGCACTTGTCGATGTTGAAAATGTTGGGTCACAAAGAGTGTTGGAGAAAGCTGGTTTCCAAAAGGAGGGAGTTCTTAGGAAGTATTTGGTCATGAAAGGAAAAAGCAGAGATATGATCATGTTTAGTGTTCTTTCTACTGATCTCCAACTTTAA

Protein sequence

>MsG0580024154.01.T01

MEETFISSKSCVKEEIIDLNQITLRPFNLSDLDDVMVWHTDVKVAKFCSWEPYTSRDDGINFIENIESKFLCCKAICIDDRAIGCILLFTPEDKSRKQSAELGYNLSSIYWGKGIVTCAVKQIIKVAFHEFSYLERLEALVDVENVGSQRVLEKAGFQKEGVLRKYLVMKGKSRDMIMFSVLSTDLQL*