AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006748.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006748.01.T01 MTR_2g014240 96.190 105 4 0 1 105 1 105 4.58e-70 205
MsG0280006748.01.T01 MTR_2g014240 96.040 101 4 0 1 101 1 101 5.31e-67 197
MsG0280006748.01.T01 MTR_2g014240 94.059 101 4 1 1 101 1 99 2.17e-53 163
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006748.01.T01 AT1G28330 55.462 119 43 4 1 110 3 120 5.33e-30 104
MsG0280006748.01.T01 AT1G28330 58.182 110 36 5 1 101 3 111 6.95e-30 103
MsG0280006748.01.T01 AT1G28330 58.182 110 36 5 1 101 3 111 6.95e-30 103
MsG0280006748.01.T01 AT1G28330 55.462 119 43 4 1 110 3 120 8.60e-30 103
MsG0280006748.01.T01 AT2G33830 54.128 109 37 6 5 109 1 100 1.96e-29 102
MsG0280006748.01.T01 AT2G33830 50.943 106 47 3 5 109 1 102 2.58e-29 102
MsG0280006748.01.T01 AT1G28330 57.798 109 36 4 1 100 3 110 1.13e-28 101
MsG0280006748.01.T01 AT1G28330 57.798 109 36 4 1 100 3 110 1.13e-28 101
MsG0280006748.01.T01 AT5G44300 53.398 103 44 4 1 101 3 103 3.13e-28 99.8

Find 63 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAAGTTTGTCTAACATTT+TGG 0.088107 2:+5787036 None:intergenic
GATGTGCCACCAAAACTTTC+TGG 0.166653 2:-5786213 MsG0280006748.01.T01:three_prime_UTR
TCCAAGTGTAACGAGATATT+TGG 0.192173 2:+5787209 None:intergenic
TACAATATTTAGAAGAAAAT+AGG 0.251306 2:+5785933 None:intergenic
AAAATATCTACGGCTAAAAT+TGG 0.265060 2:+5787168 None:intergenic
TTAGCTAGATAAACCTATTA+AGG 0.299960 2:+5786020 None:intergenic
CCAAAATGTTAGACAAACTT+TGG 0.309017 2:-5787036 MsG0280006748.01.T01:exon
TGGAGGAGCGTGTTCAATCC+TGG 0.327841 2:-5786685 MsG0280006748.01.T01:CDS
TTATCTAGCTTTGCCTTAAT+AGG 0.332499 2:-5786033 MsG0280006748.01.T01:three_prime_UTR
CACGTAAAGTTGACAACGTT+TGG 0.347744 2:-5786705 MsG0280006748.01.T01:CDS
CTTCTAAATATTGTAACAAA+TGG 0.375074 2:-5785925 MsG0280006748.01.T01:three_prime_UTR
GCAATCAACCCAGAAAGTTT+TGG 0.376912 2:+5786204 None:intergenic
TCAAGGCCACGCTCAGGTTG+AGG 0.384188 2:+5786995 None:intergenic
CAAAATGTTAGACAAACTTT+GGG 0.385846 2:-5787035 MsG0280006748.01.T01:exon
CTTTGGGATGATACCGTTGC+TGG 0.399829 2:-5787019 MsG0280006748.01.T01:CDS
CCAAGTGTAACGAGATATTT+GGG 0.415710 2:+5787210 None:intergenic
ATAATTGATAGTATAGTATG+AGG 0.431721 2:+5787097 None:intergenic
AACTCTGCTACTAAGACAAT+TGG 0.434795 2:-5786658 MsG0280006748.01.T01:CDS
GTTTATACCTTTGATGATGC+TGG 0.439938 2:+5786952 None:intergenic
CCCAAATATCTCGTTACACT+TGG 0.457056 2:-5787210 MsG0280006748.01.T01:five_prime_UTR
CAAGGCCACGCTCAGGTTGA+GGG 0.457180 2:+5786996 None:intergenic
ATGTGCCACCAAAACTTTCT+GGG 0.468880 2:-5786212 MsG0280006748.01.T01:three_prime_UTR
TGTATAGTTCTTCTAACAAA+AGG 0.474590 2:+5786402 None:intergenic
TGAGGTGTCGTTGGAGTCGC+CGG 0.474823 2:+5786733 None:intergenic
GATGGAATTGCACAGTTACA+TGG 0.475449 2:+5785877 None:intergenic
GGAGGAGCGTGTTCAATCCT+GGG 0.490368 2:-5786684 MsG0280006748.01.T01:CDS
TGGCAGACGATCAACAAAAC+AGG 0.493537 2:+5786168 None:intergenic
TCAACTTTACGTGCCGATTG+AGG 0.495412 2:+5786715 None:intergenic
GACTCCAACGACACCTCAAT+CGG 0.495837 2:-5786728 MsG0280006748.01.T01:CDS
GTTGGAGTCGCCGGTGTTGT+CGG 0.498433 2:+5786742 None:intergenic
GCCGGTGTTGTCGGTGTCGA+AGG 0.500942 2:+5786751 None:intergenic
AGCTTCTCAAGGCCACGCTC+AGG 0.504351 2:+5786989 None:intergenic
TTAGTAGCAGAGTTGCTCCC+AGG 0.506672 2:+5786667 None:intergenic
ATGCAAATTATAAGAGTGGT+TGG 0.510658 2:+5786560 None:intergenic
GTGATCGCAGGATGTACAGC+GGG 0.519197 2:-5786258 MsG0280006748.01.T01:three_prime_UTR
TTACGTGTCGTGATCTGATC+GGG 0.520466 2:-5786129 MsG0280006748.01.T01:three_prime_UTR
AGCGTGGCCTTGAGAAGCTT+AGG 0.531934 2:-5786985 MsG0280006748.01.T01:CDS
GGATGTACAGCGGGGACACA+AGG 0.536816 2:-5786249 MsG0280006748.01.T01:three_prime_UTR
CGTGCCGATTGAGGTGTCGT+TGG 0.538925 2:+5786724 None:intergenic
AGTGATGCTTAAAATATCTA+CGG 0.543333 2:+5787158 None:intergenic
GTTACGTGTCGTGATCTGAT+CGG 0.558661 2:-5786130 MsG0280006748.01.T01:three_prime_UTR
ATGCATGCAAATTATAAGAG+TGG 0.570650 2:+5786556 None:intergenic
TAATTGATAGTATAGTATGA+GGG 0.580196 2:+5787098 None:intergenic
CTCAGGTTGAGGGCCAGCAA+CGG 0.587677 2:+5787006 None:intergenic
GTAAAGTTGACAACGTTTGG+AGG 0.589302 2:-5786702 MsG0280006748.01.T01:CDS
GCTGGCCCTCAACCTGAGCG+TGG 0.589408 2:-5787001 MsG0280006748.01.T01:CDS
ACATACCAATCATAGACAGT+GGG 0.591016 2:+5786601 None:intergenic
TTAGCTTCCTAAGCTTCTCA+AGG 0.592667 2:+5786978 None:intergenic
GCCTTCGACACCGACAACAC+CGG 0.593074 2:-5786752 MsG0280006748.01.T01:CDS
TATACCTTTGATGATGCTGG+TGG 0.618859 2:+5786955 None:intergenic
CTAACCACCAGCATCATCAA+AGG 0.620330 2:-5786959 MsG0280006748.01.T01:intron
ACAAAACTCAAATTAATACA+AGG 0.623205 2:+5786055 None:intergenic
GATCAGATCACGACACGTAA+CGG 0.626020 2:+5786132 None:intergenic
GATTAATTAACACTTACAGA+AGG 0.629797 2:+5785984 None:intergenic
TGTAACGAGATATTTGGGAG+TGG 0.642896 2:+5787215 None:intergenic
ATTGGGTTGTATGTGATCGC+AGG 0.652577 2:-5786270 MsG0280006748.01.T01:three_prime_UTR
AACATACCAATCATAGACAG+TGG 0.664521 2:+5786600 None:intergenic
TGTTAGAAGAACTATACATG+TGG 0.680047 2:-5786397 MsG0280006748.01.T01:three_prime_UTR
CATACCAATCATAGACAGTG+GGG 0.727901 2:+5786602 None:intergenic
TGTGATCGCAGGATGTACAG+CGG 0.734662 2:-5786259 MsG0280006748.01.T01:three_prime_UTR
ATACCAATCATAGACAGTGG+GGG 0.749000 2:+5786603 None:intergenic
TGATCGCAGGATGTACAGCG+GGG 0.761714 2:-5786257 MsG0280006748.01.T01:three_prime_UTR
GTAACGGTGATACAGACACG+TGG 0.790753 2:+5786148 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTCATTATATTATAGT+TGG + Chr2:5786204-5786223 None:intergenic 10.0%
!! ACAAACATATAAAAATGAAT+TGG - Chr2:5786813-5786832 MsG0280006748.01.T01:intron 15.0%
!! CAAACATATAAAAATGAATT+GGG - Chr2:5786814-5786833 MsG0280006748.01.T01:intron 15.0%
!! TACAATATTTAGAAGAAAAT+AGG + Chr2:5787171-5787190 None:intergenic 15.0%
!!! GTTGATTTTAATTTGATTTT+GGG - Chr2:5786281-5786300 MsG0280006748.01.T01:three_prime_UTR 15.0%
!!! TGTTGATTTTAATTTGATTT+TGG - Chr2:5786280-5786299 MsG0280006748.01.T01:three_prime_UTR 15.0%
!!! TTGATTCTATATTTTATTTG+TGG - Chr2:5786634-5786653 MsG0280006748.01.T01:CDS 15.0%
!! ACAAAACTCAAATTAATACA+AGG + Chr2:5787049-5787068 None:intergenic 20.0%
!! CTTCTAAATATTGTAACAAA+TGG - Chr2:5787176-5787195 MsG0280006748.01.T01:five_prime_UTR 20.0%
!!! ATAATTGATAGTATAGTATG+AGG + Chr2:5786007-5786026 None:intergenic 20.0%
!!! TAATTGATAGTATAGTATGA+GGG + Chr2:5786006-5786025 None:intergenic 20.0%
! AAAATATCTACGGCTAAAAT+TGG + Chr2:5785936-5785955 None:intergenic 25.0%
! AGTGATGCTTAAAATATCTA+CGG + Chr2:5785946-5785965 None:intergenic 25.0%
! CAAAATGTTAGACAAACTTT+GGG - Chr2:5786066-5786085 MsG0280006748.01.T01:three_prime_UTR 25.0%
! GATTAATTAACACTTACAGA+AGG + Chr2:5787120-5787139 None:intergenic 25.0%
! TGTATAGTTCTTCTAACAAA+AGG + Chr2:5786702-5786721 None:intergenic 25.0%
! TTAGCTAGATAAACCTATTA+AGG + Chr2:5787084-5787103 None:intergenic 25.0%
ATGCAAATTATAAGAGTGGT+TGG + Chr2:5786544-5786563 None:intergenic 30.0%
ATGCATGCAAATTATAAGAG+TGG + Chr2:5786548-5786567 None:intergenic 30.0%
CCAAAATGTTAGACAAACTT+TGG - Chr2:5786065-5786084 MsG0280006748.01.T01:three_prime_UTR 30.0%
TGTTAGAAGAACTATACATG+TGG - Chr2:5786704-5786723 MsG0280006748.01.T01:CDS 30.0%
! CCAAAGTTTGTCTAACATTT+TGG + Chr2:5786068-5786087 None:intergenic 30.0%
!! TTATCTAGCTTTGCCTTAAT+AGG - Chr2:5787068-5787087 MsG0280006748.01.T01:five_prime_UTR 30.0%
!!! CTAACATTTTGGAACTAAGA+TGG + Chr2:5786057-5786076 None:intergenic 30.0%
AACATACCAATCATAGACAG+TGG + Chr2:5786504-5786523 None:intergenic 35.0%
AACTCTGCTACTAAGACAAT+TGG - Chr2:5786443-5786462 MsG0280006748.01.T01:three_prime_UTR 35.0%
ACATACCAATCATAGACAGT+GGG + Chr2:5786503-5786522 None:intergenic 35.0%
CCAAGTGTAACGAGATATTT+GGG + Chr2:5785894-5785913 None:intergenic 35.0%
TCCAAGTGTAACGAGATATT+TGG + Chr2:5785895-5785914 None:intergenic 35.0%
!! GTTTATACCTTTGATGATGC+TGG + Chr2:5786152-5786171 None:intergenic 35.0%
!! TTGATTTTGGGTGTAGATGA+AGG - Chr2:5786293-5786312 MsG0280006748.01.T01:three_prime_UTR 35.0%
ATACCAATCATAGACAGTGG+GGG + Chr2:5786501-5786520 None:intergenic 40.0%
ATGTGCCACCAAAACTTTCT+GGG - Chr2:5786889-5786908 MsG0280006748.01.T01:intron 40.0%
CACGTAAAGTTGACAACGTT+TGG - Chr2:5786396-5786415 MsG0280006748.01.T01:three_prime_UTR 40.0%
CATACCAATCATAGACAGTG+GGG + Chr2:5786502-5786521 None:intergenic 40.0%
CCCAAATATCTCGTTACACT+TGG - Chr2:5785891-5785910 MsG0280006748.01.T01:three_prime_UTR 40.0%
GTAAAGTTGACAACGTTTGG+AGG - Chr2:5786399-5786418 MsG0280006748.01.T01:three_prime_UTR 40.0%
TTAGCTTCCTAAGCTTCTCA+AGG + Chr2:5786126-5786145 None:intergenic 40.0%
! GCAATCAACCCAGAAAGTTT+TGG + Chr2:5786900-5786919 None:intergenic 40.0%
!! ATCAACCCAGAAAGTTTTGG+TGG + Chr2:5786897-5786916 None:intergenic 40.0%
!! ATTTTGGGTGTAGATGAAGG+AGG - Chr2:5786296-5786315 MsG0280006748.01.T01:three_prime_UTR 40.0%
!! TATACCTTTGATGATGCTGG+TGG + Chr2:5786149-5786168 None:intergenic 40.0%
CTAACCACCAGCATCATCAA+AGG - Chr2:5786142-5786161 MsG0280006748.01.T01:three_prime_UTR 45.0%
GATCAGATCACGACACGTAA+CGG + Chr2:5786972-5786991 None:intergenic 45.0%
GATGTGCCACCAAAACTTTC+TGG - Chr2:5786888-5786907 MsG0280006748.01.T01:intron 45.0%
GTTACGTGTCGTGATCTGAT+CGG - Chr2:5786971-5786990 MsG0280006748.01.T01:CDS 45.0%
TGGCAGACGATCAACAAAAC+AGG + Chr2:5786936-5786955 None:intergenic 45.0%
TTACGTGTCGTGATCTGATC+GGG - Chr2:5786972-5786991 MsG0280006748.01.T01:CDS 45.0%
! ATTGGGTTGTATGTGATCGC+AGG - Chr2:5786831-5786850 MsG0280006748.01.T01:intron 45.0%
!! AAGTTTTGGTGGCACATCAG+CGG + Chr2:5786886-5786905 None:intergenic 45.0%
!! TCAACTTTACGTGCCGATTG+AGG + Chr2:5786389-5786408 None:intergenic 45.0%
ACACCCCCACTGTCTATGAT+TGG - Chr2:5786495-5786514 MsG0280006748.01.T01:three_prime_UTR 50.0%
GACTCCAACGACACCTCAAT+CGG - Chr2:5786373-5786392 MsG0280006748.01.T01:three_prime_UTR 50.0%
GTAACGGTGATACAGACACG+TGG + Chr2:5786956-5786975 None:intergenic 50.0%
TGTGATCGCAGGATGTACAG+CGG - Chr2:5786842-5786861 MsG0280006748.01.T01:intron 50.0%
TTAGTAGCAGAGTTGCTCCC+AGG + Chr2:5786437-5786456 None:intergenic 50.0%
! ATCATAGACAGTGGGGGTGT+TGG + Chr2:5786495-5786514 None:intergenic 50.0%
!! CTTTGGGATGATACCGTTGC+TGG - Chr2:5786082-5786101 MsG0280006748.01.T01:three_prime_UTR 50.0%
!! TCATAGACAGTGGGGGTGTT+GGG + Chr2:5786494-5786513 None:intergenic 50.0%
GTGATCGCAGGATGTACAGC+GGG - Chr2:5786843-5786862 MsG0280006748.01.T01:intron 55.0%
TGATCGCAGGATGTACAGCG+GGG - Chr2:5786844-5786863 MsG0280006748.01.T01:intron 55.0%
! AGCGTGGCCTTGAGAAGCTT+AGG - Chr2:5786116-5786135 MsG0280006748.01.T01:three_prime_UTR 55.0%
! GGAGGAGCGTGTTCAATCCT+GGG - Chr2:5786417-5786436 MsG0280006748.01.T01:three_prime_UTR 55.0%
! TGGAGGAGCGTGTTCAATCC+TGG - Chr2:5786416-5786435 MsG0280006748.01.T01:three_prime_UTR 55.0%
!! CATAGACAGTGGGGGTGTTG+GGG + Chr2:5786493-5786512 None:intergenic 55.0%
AGCTTCTCAAGGCCACGCTC+AGG + Chr2:5786115-5786134 None:intergenic 60.0%
GCCTTCGACACCGACAACAC+CGG - Chr2:5786349-5786368 MsG0280006748.01.T01:three_prime_UTR 60.0%
GGATGTACAGCGGGGACACA+AGG - Chr2:5786852-5786871 MsG0280006748.01.T01:intron 60.0%
! TCAAGGCCACGCTCAGGTTG+AGG + Chr2:5786109-5786128 None:intergenic 60.0%
!! CAAGGCCACGCTCAGGTTGA+GGG + Chr2:5786108-5786127 None:intergenic 60.0%
!! CGTGCCGATTGAGGTGTCGT+TGG + Chr2:5786380-5786399 None:intergenic 60.0%
!! CTCAGGTTGAGGGCCAGCAA+CGG + Chr2:5786098-5786117 None:intergenic 60.0%
!! GTTGGAGTCGCCGGTGTTGT+CGG + Chr2:5786362-5786381 None:intergenic 60.0%
!! TGAGGTGTCGTTGGAGTCGC+CGG + Chr2:5786371-5786390 None:intergenic 60.0%
!! ACAGTGGGGGTGTTGGGGAG+TGG + Chr2:5786488-5786507 None:intergenic 65.0%
!! GCCGGTGTTGTCGGTGTCGA+AGG + Chr2:5786353-5786372 None:intergenic 65.0%
GCTGGCCCTCAACCTGAGCG+TGG - Chr2:5786100-5786119 MsG0280006748.01.T01:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 5785888 5787235 5785888 ID=MsG0280006748.01;Name=MsG0280006748.01
Chr2 mRNA 5785888 5787235 5785888 ID=MsG0280006748.01.T01;Parent=MsG0280006748.01;Name=MsG0280006748.01.T01;_AED=0.47;_eAED=0.47;_QI=182|1|1|1|1|1|2|679|113
Chr2 exon 5785888 5786814 5785888 ID=MsG0280006748.01.T01:exon:2354;Parent=MsG0280006748.01.T01
Chr2 exon 5786960 5787235 5786960 ID=MsG0280006748.01.T01:exon:2353;Parent=MsG0280006748.01.T01
Chr2 five_prime_UTR 5787054 5787235 5787054 ID=MsG0280006748.01.T01:five_prime_utr;Parent=MsG0280006748.01.T01
Chr2 CDS 5786960 5787053 5786960 ID=MsG0280006748.01.T01:cds;Parent=MsG0280006748.01.T01
Chr2 CDS 5786567 5786814 5786567 ID=MsG0280006748.01.T01:cds;Parent=MsG0280006748.01.T01
Chr2 three_prime_UTR 5785888 5786566 5785888 ID=MsG0280006748.01.T01:three_prime_utr;Parent=MsG0280006748.01.T01
Gene Sequence

>MsG0280006748.01.T01

ATGTTAGACAAACTTTGGGATGATACCGTTGCTGGCCCTCAACCTGAGCGTGGCCTTGAGAAGCTTAGGAAGCTAACCACCAGCATCATCAAAGATGAAGGAGGAAGTGCTCAGTTAATGAGAAACACGTCGTTGCCTTCGACACCGACAACACCGGCGACTCCAACGACACCTCAATCGGCACGTAAAGTTGACAACGTTTGGAGGAGCGTGTTCAATCCTGGGAGCAACTCTGCTACTAAGACAATTGGTGCTCATATGTTTGACAAACCACTCCCCAACACCCCCACTGTCTATGATTGGTATGTTATTATATATTCACAAAACCAACCACTCTTATAA

Protein sequence

>MsG0280006748.01.T01

MLDKLWDDTVAGPQPERGLEKLRKLTTSIIKDEGGSAQLMRNTSLPSTPTTPATPTTPQSARKVDNVWRSVFNPGSNSATKTIGAHMFDKPLPNTPTVYDWYVIIYSQNQPLL*