AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006875.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006875.01.T01 MTR_2g015850 70.312 192 7 1 1 142 173 364 9.03e-88 260
MsG0280006875.01.T01 MTR_2g015840 60.563 142 43 2 1 142 144 272 6.77e-56 176
MsG0280006875.01.T01 MTR_4g107930 46.154 195 51 2 1 141 150 344 7.74e-52 167
MsG0280006875.01.T01 MTR_4g079470 46.154 195 51 2 1 141 150 344 3.35e-51 166
MsG0280006875.01.T01 MTR_4g079800 45.128 195 53 2 1 141 150 344 4.11e-51 166
MsG0280006875.01.T01 MTR_4g079770 46.154 195 51 2 1 141 150 344 4.57e-51 166
MsG0280006875.01.T01 MTR_5g022560 46.154 195 51 2 1 141 149 343 1.25e-50 164
MsG0280006875.01.T01 MTR_4g047610 45.641 195 52 2 1 141 148 342 5.07e-50 163
MsG0280006875.01.T01 MTR_7g078600 42.708 192 59 2 2 142 147 338 2.60e-45 150
MsG0280006875.01.T01 MTR_4g104370 41.361 191 60 2 2 140 152 342 2.87e-44 148
MsG0280006875.01.T01 MTR_4g080360 43.229 192 56 3 2 140 149 340 3.60e-44 148
MsG0280006875.01.T01 MTR_1g023810 41.579 190 58 4 2 139 73 261 8.33e-43 142
MsG0280006875.01.T01 MTR_3g075390 41.146 192 60 3 2 140 152 343 3.14e-42 143
MsG0280006875.01.T01 MTR_4g080730 41.451 193 59 3 2 141 148 339 1.60e-41 141
MsG0280006875.01.T01 MTR_4g080700 40.933 193 60 3 2 141 148 339 3.72e-41 140
MsG0280006875.01.T01 MTR_4g079440 41.538 195 59 4 2 142 152 345 4.23e-40 137
MsG0280006875.01.T01 MTR_3g116080 40.933 193 60 4 2 141 156 347 2.92e-39 135
MsG0280006875.01.T01 MTR_2g090890 39.378 193 62 4 2 140 144 335 5.49e-39 134
MsG0280006875.01.T01 MTR_3g102220 41.451 193 58 5 2 140 169 360 1.13e-38 136
MsG0280006875.01.T01 MTR_3g112430 40.933 193 60 4 2 141 150 341 1.60e-38 135
MsG0280006875.01.T01 MTR_7g079230 39.062 192 66 2 2 142 145 336 1.75e-38 133
MsG0280006875.01.T01 MTR_2g075830 43.077 195 57 3 1 141 146 340 2.98e-38 132
MsG0280006875.01.T01 MTR_1g107550 42.487 193 56 5 2 140 163 354 6.85e-38 134
MsG0280006875.01.T01 MTR_1g018840 40.415 193 60 5 2 140 169 360 1.37e-36 130
MsG0280006875.01.T01 MTR_4g094918 40.415 193 60 5 2 140 149 340 2.67e-36 127
MsG0280006875.01.T01 MTR_1g018840 40.415 193 60 5 2 140 169 360 3.46e-36 129
MsG0280006875.01.T01 MTR_7g078580 53.608 97 45 0 44 140 4 100 1.17e-33 115
MsG0280006875.01.T01 MTR_2g083930 36.410 195 70 4 2 142 150 344 3.15e-33 119
MsG0280006875.01.T01 MTR_2g067560 41.489 188 55 7 1 134 136 322 7.63e-33 122
MsG0280006875.01.T01 MTR_2g066980 41.317 167 67 6 2 142 158 319 4.22e-31 113
MsG0280006875.01.T01 MTR_2g064475 40.513 195 56 9 2 141 162 351 3.16e-30 112
MsG0280006875.01.T01 MTR_7g079130 53.846 91 41 1 51 140 6 96 2.89e-29 102
MsG0280006875.01.T01 MTR_2g016270 34.021 194 73 5 2 141 148 340 1.13e-25 100
MsG0280006875.01.T01 MTR_2g016180 34.021 194 73 5 2 141 163 355 1.13e-25 101
MsG0280006875.01.T01 MTR_2g067900 36.126 191 68 5 2 139 134 323 3.46e-24 95.5
MsG0280006875.01.T01 MTR_2g015930 33.333 192 74 5 2 140 167 357 1.08e-23 94.7
MsG0280006875.01.T01 MTR_8g086470 35.938 192 67 6 3 140 166 355 1.48e-23 94.4
MsG0280006875.01.T01 MTR_4g065077 35.938 192 67 6 3 140 166 355 1.54e-23 94.4
MsG0280006875.01.T01 MTR_4g125300 47.748 111 48 6 37 142 244 349 2.08e-22 90.9
MsG0280006875.01.T01 MTR_2g016380 39.024 123 69 4 23 141 15 135 2.78e-19 81.3
MsG0280006875.01.T01 MTR_2g015920 44.000 100 51 3 45 140 259 357 6.02e-19 81.6
MsG0280006875.01.T01 MTR_4g072790 33.775 151 68 4 3 122 156 305 7.74e-19 80.9
MsG0280006875.01.T01 MTR_4g072340 30.968 155 71 4 3 122 156 309 9.03e-17 75.5
MsG0280006875.01.T01 MTR_4g073660 30.968 155 71 4 3 122 155 308 1.11e-16 75.1
MsG0280006875.01.T01 MTR_7g111060 37.634 93 52 3 43 133 242 330 2.98e-15 71.2
MsG0280006875.01.T01 MTR_7g111050 36.842 95 58 2 43 135 239 333 4.68e-15 70.9
MsG0280006875.01.T01 MTR_7g079260 48.611 72 21 2 67 138 7 62 1.52e-14 65.5
MsG0280006875.01.T01 MTR_7g078630 51.613 62 30 0 40 101 889 950 4.16e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006875.01.T01 AT3G49340 46.842 190 51 3 1 140 150 339 1.11e-51 167
MsG0280006875.01.T01 AT2G34080 46.073 191 52 2 1 140 153 343 1.55e-51 167
MsG0280006875.01.T01 AT5G45890 45.361 194 54 2 1 142 153 346 8.14e-51 165
MsG0280006875.01.T01 AT2G27420 44.103 195 53 3 2 140 152 346 2.98e-47 156
MsG0280006875.01.T01 AT3G48340 45.833 192 50 4 2 140 152 342 5.87e-46 153
MsG0280006875.01.T01 AT3G48350 43.299 194 56 3 2 141 150 343 2.26e-45 151
MsG0280006875.01.T01 AT1G29080 42.932 191 57 3 2 140 154 344 1.02e-44 149
MsG0280006875.01.T01 AT1G29090 43.229 192 57 3 1 140 162 353 6.62e-44 147
MsG0280006875.01.T01 AT1G20850 42.487 193 57 4 2 141 162 353 1.08e-41 142
MsG0280006875.01.T01 AT5G50260 41.667 192 59 3 2 140 150 341 1.31e-41 142
MsG0280006875.01.T01 AT3G19390 42.784 194 56 4 2 141 153 345 7.77e-41 141
MsG0280006875.01.T01 AT4G36880 41.969 193 57 5 2 140 169 360 8.27e-41 140
MsG0280006875.01.T01 AT4G35350 41.451 193 59 4 2 141 161 352 8.43e-41 139
MsG0280006875.01.T01 AT4G11310 40.104 192 61 3 2 140 161 351 2.20e-40 139
MsG0280006875.01.T01 AT1G06260 44.271 192 53 4 2 140 151 341 3.33e-40 137
MsG0280006875.01.T01 AT4G11320 39.583 192 62 3 2 140 168 358 3.54e-40 138
MsG0280006875.01.T01 AT4G11320 39.583 192 62 3 2 140 168 358 3.54e-40 138
MsG0280006875.01.T01 AT3G19400 42.857 196 55 4 2 141 154 348 9.35e-40 137
MsG0280006875.01.T01 AT4G23520 41.969 193 57 4 2 140 157 348 6.53e-39 134
MsG0280006875.01.T01 AT5G43060 41.146 192 59 4 2 140 162 352 1.72e-38 135
MsG0280006875.01.T01 AT1G09850 39.062 192 63 4 2 140 142 332 4.38e-36 129
MsG0280006875.01.T01 AT1G47128 38.021 192 65 4 2 140 161 351 6.67e-36 129
MsG0280006875.01.T01 AT1G29110 54.286 105 45 3 40 142 231 334 3.95e-31 114
MsG0280006875.01.T01 AT3G43960 40.625 128 65 3 14 140 228 345 7.12e-26 100
MsG0280006875.01.T01 AT5G60360 36.869 198 63 8 2 142 165 357 1.25e-25 99.8
MsG0280006875.01.T01 AT5G60360 35.859 198 64 7 2 142 165 356 3.42e-24 95.9
MsG0280006875.01.T01 AT3G45310 35.354 198 66 8 2 142 165 357 7.99e-23 92.4
MsG0280006875.01.T01 AT5G60360 35.795 176 55 6 2 120 165 339 1.01e-21 89.4
MsG0280006875.01.T01 AT3G45310 34.343 198 67 7 2 142 165 356 2.10e-21 88.6
MsG0280006875.01.T01 AT1G02305 35.052 97 62 1 40 135 243 339 4.76e-16 73.6
MsG0280006875.01.T01 AT1G02300 39.130 92 50 3 44 133 263 350 1.65e-15 72.4
MsG0280006875.01.T01 AT3G48350 38.211 123 22 3 2 70 150 272 5.08e-15 70.5
MsG0280006875.01.T01 AT4G01610 34.343 99 60 2 36 133 236 330 1.92e-14 69.3
MsG0280006875.01.T01 AT4G01610 34.343 99 60 2 36 133 236 330 2.02e-14 68.9

Find 33 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATGGGAGATGTTTATTC+AGG 0.305565 2:+7473329 MsG0280006875.01.T01:CDS
ATTGGTTGATTAAGAATTCT+TGG 0.312011 2:+7473423 MsG0280006875.01.T01:CDS
AGAATTCTTGGGGTAAAGAT+TGG 0.334902 2:+7473435 MsG0280006875.01.T01:CDS
TTGGTTGATTAAGAATTCTT+GGG 0.374775 2:+7473424 MsG0280006875.01.T01:CDS
GAATTCTTGGGGTAAAGATT+GGG 0.376941 2:+7473436 MsG0280006875.01.T01:CDS
CTCATTATCTGAGCAACAAT+TGG 0.401955 2:+7473038 MsG0280006875.01.T01:CDS
ACTGCAATGCCAACTGATAC+TGG 0.406208 2:-7473287 None:intergenic
TAGCAGTTGTTGTTCATCAT+TGG 0.413217 2:-7473246 None:intergenic
TCAGTCGTAGCTGCTGTAGA+AGG 0.446164 2:+7472985 MsG0280006875.01.T01:CDS
GGTAAAGATTGGGGTGAAGA+AGG 0.460104 2:+7473446 MsG0280006875.01.T01:CDS
TGATGAATTTCAACATTACA+TGG 0.464191 2:+7473313 MsG0280006875.01.T01:CDS
GCAGTTACTGCAGTTGGTTA+CGG 0.478465 2:+7473377 MsG0280006875.01.T01:CDS
AGTTGTTGTTCATCATTGGC+AGG 0.496843 2:-7473242 None:intergenic
AACCATGCAGTTACTGCAGT+TGG 0.506721 2:+7473371 MsG0280006875.01.T01:CDS
AGGTTACATGAAGTTGTTGA+GGG 0.508816 2:+7473466 MsG0280006875.01.T01:CDS
GCAATGCCACATTGACCTCC+TGG 0.513337 2:-7473500 None:intergenic
TTGAGGGAAAGTGGTGAACC+AGG 0.520538 2:+7473482 MsG0280006875.01.T01:CDS
AATTCTTGGGGTAAAGATTG+GGG 0.530447 2:+7473437 MsG0280006875.01.T01:CDS
GGTTACGGTGTAAGTGAAGA+TGG 0.532156 2:+7473392 MsG0280006875.01.T01:CDS
GTGAAGATGGAACTAAGTAT+TGG 0.533019 2:+7473405 MsG0280006875.01.T01:CDS
ATGAAGTTGTTGAGGGAAAG+TGG 0.542145 2:+7473473 MsG0280006875.01.T01:CDS
AAGGTTACATGAAGTTGTTG+AGG 0.582698 2:+7473465 MsG0280006875.01.T01:CDS
GACTGCGATGAACAGACAAT+GGG 0.586011 2:+7473063 MsG0280006875.01.T01:CDS
TGGTTGATTAAGAATTCTTG+GGG 0.586376 2:+7473425 MsG0280006875.01.T01:CDS
GATGTTTATTCAGGAACATG+TGG 0.602261 2:+7473338 MsG0280006875.01.T01:CDS
AACCAACTGCAGTAACTGCA+TGG 0.607601 2:-7473373 None:intergenic
GTATCAGTTGGCATTGCAGT+CGG 0.623362 2:+7473290 MsG0280006875.01.T01:CDS
GATGAATTTCAACATTACAT+GGG 0.623570 2:+7473314 MsG0280006875.01.T01:CDS
GCACAACAACCAGTATCAGT+TGG 0.635741 2:+7473278 MsG0280006875.01.T01:CDS
GGTGCTGTGAAAATCAAGAC+TGG 0.639937 2:+7473006 MsG0280006875.01.T01:CDS
TGACTGCGATGAACAGACAA+TGG 0.644680 2:+7473062 MsG0280006875.01.T01:CDS
GGTGAACCAGGAGGTCAATG+TGG 0.664219 2:+7473494 MsG0280006875.01.T01:CDS
AGGGAAAGTGGTGAACCAGG+AGG 0.728920 2:+7473485 MsG0280006875.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCATTTGATCATTTAAT+TGG - Chr2:7473190-7473209 None:intergenic 15.0%
!! AAATATATAATACAAACCCA+AGG + Chr2:7473116-7473135 MsG0280006875.01.T01:intron 20.0%
!! GGTTTGTATTATATATTTGA+AGG - Chr2:7473114-7473133 None:intergenic 20.0%
! ATTGGTTGATTAAGAATTCT+TGG + Chr2:7473423-7473442 MsG0280006875.01.T01:CDS 25.0%
! GATGAATTTCAACATTACAT+GGG + Chr2:7473314-7473333 MsG0280006875.01.T01:CDS 25.0%
! TGATGAATTTCAACATTACA+TGG + Chr2:7473313-7473332 MsG0280006875.01.T01:CDS 25.0%
! TTGGTTGATTAAGAATTCTT+GGG + Chr2:7473424-7473443 MsG0280006875.01.T01:CDS 25.0%
ACAGATTATCCATATCAAGA+AGG + Chr2:7473152-7473171 MsG0280006875.01.T01:intron 30.0%
TGGTTGATTAAGAATTCTTG+GGG + Chr2:7473425-7473444 MsG0280006875.01.T01:CDS 30.0%
AAGGTTACATGAAGTTGTTG+AGG + Chr2:7473465-7473484 MsG0280006875.01.T01:CDS 35.0%
AATTCTTGGGGTAAAGATTG+GGG + Chr2:7473437-7473456 MsG0280006875.01.T01:CDS 35.0%
AGAATTCTTGGGGTAAAGAT+TGG + Chr2:7473435-7473454 MsG0280006875.01.T01:CDS 35.0%
AGGTTACATGAAGTTGTTGA+GGG + Chr2:7473466-7473485 MsG0280006875.01.T01:CDS 35.0%
CTCATTATCTGAGCAACAAT+TGG + Chr2:7473038-7473057 MsG0280006875.01.T01:CDS 35.0%
GAATTCTTGGGGTAAAGATT+GGG + Chr2:7473436-7473455 MsG0280006875.01.T01:CDS 35.0%
GATGTTTATTCAGGAACATG+TGG + Chr2:7473338-7473357 MsG0280006875.01.T01:CDS 35.0%
GTGAAGATGGAACTAAGTAT+TGG + Chr2:7473405-7473424 MsG0280006875.01.T01:CDS 35.0%
TACATGGGAGATGTTTATTC+AGG + Chr2:7473329-7473348 MsG0280006875.01.T01:CDS 35.0%
TGTCTCACTAACAATACCTT+GGG - Chr2:7473135-7473154 None:intergenic 35.0%
! TAGCAGTTGTTGTTCATCAT+TGG - Chr2:7473249-7473268 None:intergenic 35.0%
ATGAAGTTGTTGAGGGAAAG+TGG + Chr2:7473473-7473492 MsG0280006875.01.T01:CDS 40.0%
CTGTCTCACTAACAATACCT+TGG - Chr2:7473136-7473155 None:intergenic 40.0%
GTCTGACTACCTTCTTGATA+TGG - Chr2:7473164-7473183 None:intergenic 40.0%
! AGTTGTTGTTCATCATTGGC+AGG - Chr2:7473245-7473264 None:intergenic 40.0%
AACCAACTGCAGTAACTGCA+TGG - Chr2:7473376-7473395 None:intergenic 45.0%
AACCATGCAGTTACTGCAGT+TGG + Chr2:7473371-7473390 MsG0280006875.01.T01:CDS 45.0%
ACTGCAATGCCAACTGATAC+TGG - Chr2:7473290-7473309 None:intergenic 45.0%
GACTGCGATGAACAGACAAT+GGG + Chr2:7473063-7473082 MsG0280006875.01.T01:CDS 45.0%
GCACAACAACCAGTATCAGT+TGG + Chr2:7473278-7473297 MsG0280006875.01.T01:CDS 45.0%
GCAGTTACTGCAGTTGGTTA+CGG + Chr2:7473377-7473396 MsG0280006875.01.T01:CDS 45.0%
GGTAAAGATTGGGGTGAAGA+AGG + Chr2:7473446-7473465 MsG0280006875.01.T01:CDS 45.0%
GGTTACGGTGTAAGTGAAGA+TGG + Chr2:7473392-7473411 MsG0280006875.01.T01:CDS 45.0%
GTATCAGTTGGCATTGCAGT+CGG + Chr2:7473290-7473309 MsG0280006875.01.T01:CDS 45.0%
TGACTGCGATGAACAGACAA+TGG + Chr2:7473062-7473081 MsG0280006875.01.T01:CDS 45.0%
! GGTGCTGTGAAAATCAAGAC+TGG + Chr2:7473006-7473025 MsG0280006875.01.T01:CDS 45.0%
CGATGAACAGACAATGGGTG+TGG + Chr2:7473068-7473087 MsG0280006875.01.T01:intron 50.0%
TCAGTCGTAGCTGCTGTAGA+AGG + Chr2:7472985-7473004 MsG0280006875.01.T01:CDS 50.0%
TGAACAGACAATGGGTGTGG+TGG + Chr2:7473071-7473090 MsG0280006875.01.T01:intron 50.0%
TTGAGGGAAAGTGGTGAACC+AGG + Chr2:7473482-7473501 MsG0280006875.01.T01:CDS 50.0%
! TGGGTGTGGTGGAGGTAATA+TGG + Chr2:7473082-7473101 MsG0280006875.01.T01:intron 50.0%
ACAGACAATGGGTGTGGTGG+AGG + Chr2:7473074-7473093 MsG0280006875.01.T01:intron 55.0%
AGGGAAAGTGGTGAACCAGG+AGG + Chr2:7473485-7473504 MsG0280006875.01.T01:CDS 55.0%
GCAATGCCACATTGACCTCC+TGG - Chr2:7473503-7473522 None:intergenic 55.0%
GGTGAACCAGGAGGTCAATG+TGG + Chr2:7473494-7473513 MsG0280006875.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 7472970 7473550 7472970 ID=MsG0280006875.01;Name=MsG0280006875.01
Chr2 mRNA 7472970 7473550 7472970 ID=MsG0280006875.01.T01;Parent=MsG0280006875.01;Name=MsG0280006875.01.T01;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|1|0.5|2|0|142
Chr2 exon 7472970 7473084 7472970 ID=MsG0280006875.01.T01:exon:7883;Parent=MsG0280006875.01.T01
Chr2 exon 7473237 7473550 7473237 ID=MsG0280006875.01.T01:exon:7884;Parent=MsG0280006875.01.T01
Chr2 CDS 7472970 7473084 7472970 ID=MsG0280006875.01.T01:cds;Parent=MsG0280006875.01.T01
Chr2 CDS 7473237 7473550 7473237 ID=MsG0280006875.01.T01:cds;Parent=MsG0280006875.01.T01
Gene Sequence

>MsG0280006875.01.T01

TGTTGTTGGGCATTTTCAGTCGTAGCTGCTGTAGAAGGTGCTGTGAAAATCAAGACTGGTGAGTTGATCTCATTATCTGAGCAACAATTGGTTGACTGCGATGAACAGACAATGGACGTACCTGCCAATGATGAACAACAACTGCTACAAGCTGTAGCACAACAACCAGTATCAGTTGGCATTGCAGTCGGTGATGAATTTCAACATTACATGGGAGATGTTTATTCAGGAACATGTGGACAATCAATGAACCATGCAGTTACTGCAGTTGGTTACGGTGTAAGTGAAGATGGAACTAAGTATTGGTTGATTAAGAATTCTTGGGGTAAAGATTGGGGTGAAGAAGGTTACATGAAGTTGTTGAGGGAAAGTGGTGAACCAGGAGGTCAATGTGGCATTGCTGCACATGCTTCTTATCCCACCATATAA

Protein sequence

>MsG0280006875.01.T01

CCWAFSVVAAVEGAVKIKTGELISLSEQQLVDCDEQTMDVPANDEQQLLQAVAQQPVSVGIAVGDEFQHYMGDVYSGTCGQSMNHAVTAVGYGVSEDGTKYWLIKNSWGKDWGEEGYMKLLRESGEPGGQCGIAAHASYPTI*