AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041475.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041475.01.T01 MTR_7g111770 96.992 133 3 1 1 132 1 133 2.68e-88 253
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCTTTGTCTTCATTT+TGG 0.109870 7:-91380429 None:intergenic
ACCCACCTTCTTGTCTGTTT+TGG 0.188331 7:-91380206 None:intergenic
TTTCATTGCTTGTGAAGAAT+TGG 0.270741 7:+91380279 MsG0780041475.01.T01:CDS
AGAAAATGATGATGAGAAAA+TGG 0.284989 7:+91380141 MsG0780041475.01.T01:CDS
AAATCTTCAAGTTGAAAAGT+TGG 0.396214 7:-91380259 None:intergenic
TTCATTGCTTGTGAAGAATT+GGG 0.401669 7:+91380280 MsG0780041475.01.T01:CDS
ATCTCTTGTACTCTTTATCA+AGG 0.452854 7:-91380176 None:intergenic
GGAGCATGGAAGGAGAAAAC+AGG 0.515473 7:+91380092 None:intergenic
AGTAATGTTTCTGCTGCAGC+TGG 0.526027 7:+91380307 MsG0780041475.01.T01:CDS
CAGTAATTGAGGAGCATGGA+AGG 0.538773 7:+91380082 None:intergenic
CCTCTCCAAAACAGACAAGA+AGG 0.561940 7:+91380201 MsG0780041475.01.T01:CDS
TCCAAAACAGACAAGAAGGT+GGG 0.626074 7:+91380205 MsG0780041475.01.T01:CDS
CTCCAAAACAGACAAGAAGG+TGG 0.630444 7:+91380204 MsG0780041475.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAGGAAAAGTGTATT+TGG + Chr7:91380233-91380252 MsG0780041475.01.T01:CDS 20.0%
!! AGAAAATGATGATGAGAAAA+TGG + Chr7:91380141-91380160 MsG0780041475.01.T01:CDS 25.0%
!!! AAATCTTCAAGTTGAAAAGT+TGG - Chr7:91380262-91380281 None:intergenic 25.0%
!!! TTGTCTTCATTTTGGTTTTT+TGG - Chr7:91380424-91380443 None:intergenic 25.0%
AAACAGGAAGAGAAAAATGT+TGG + Chr7:91380108-91380127 MsG0780041475.01.T01:CDS 30.0%
ATCTCTTGTACTCTTTATCA+AGG - Chr7:91380179-91380198 None:intergenic 30.0%
TTCATTGCTTGTGAAGAATT+GGG + Chr7:91380280-91380299 MsG0780041475.01.T01:CDS 30.0%
TTTCATTGCTTGTGAAGAAT+TGG + Chr7:91380279-91380298 MsG0780041475.01.T01:CDS 30.0%
! CTTTCTCTTTGTCTTCATTT+TGG - Chr7:91380432-91380451 None:intergenic 30.0%
TTCTTCACAGCAAGAAAAAG+AGG + Chr7:91380333-91380352 MsG0780041475.01.T01:CDS 35.0%
!! TCTTTTTCTTGCTGTGAAGA+AGG - Chr7:91380334-91380353 None:intergenic 35.0%
GAAGGTGGGTGAAGAAAAAA+AGG + Chr7:91380219-91380238 MsG0780041475.01.T01:CDS 40.0%
TCCAAAACAGACAAGAAGGT+GGG + Chr7:91380205-91380224 MsG0780041475.01.T01:CDS 40.0%
CCTCTCCAAAACAGACAAGA+AGG + Chr7:91380201-91380220 MsG0780041475.01.T01:CDS 45.0%
CTCCAAAACAGACAAGAAGG+TGG + Chr7:91380204-91380223 MsG0780041475.01.T01:CDS 45.0%
! ACCCACCTTCTTGTCTGTTT+TGG - Chr7:91380209-91380228 None:intergenic 45.0%
! AGTAATGTTTCTGCTGCAGC+TGG + Chr7:91380307-91380326 MsG0780041475.01.T01:CDS 45.0%
!!! CCTTCTTGTCTGTTTTGGAG+AGG - Chr7:91380204-91380223 None:intergenic 45.0%
!!! TTTTGGTGTCACTGCTGTAG+TGG - Chr7:91380407-91380426 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 91380097 91380495 91380097 ID=MsG0780041475.01;Name=MsG0780041475.01
Chr7 mRNA 91380097 91380495 91380097 ID=MsG0780041475.01.T01;Parent=MsG0780041475.01;Name=MsG0780041475.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|132
Chr7 exon 91380097 91380495 91380097 ID=MsG0780041475.01.T01:exon:23637;Parent=MsG0780041475.01.T01
Chr7 CDS 91380097 91380495 91380097 ID=MsG0780041475.01.T01:cds;Parent=MsG0780041475.01.T01
Gene Sequence

>MsG0780041475.01.T01

ATGGAAGGAGAAAACAGGAAGAGAAAAATGTTGGAAGATGAAGAAGAAAATGATGATGAGAAAATGGAGAAGTTCTTTGCCTTGATAAAGAGTACAAGAGATATCCTCTCCAAAACAGACAAGAAGGTGGGTGAAGAAAAAAAGGAAAAGTGTATTTGGAATCCAACTTTTCAACTTGAAGATTTCATTGCTTGTGAAGAATTGGGAAAGAGTAATGTTTCTGCTGCAGCTGGTCCTTCTTCACAGCAAGAAAAAGAGGTGAAGATAATAGAGAAAGAATATGTGCAAGAAGTAGCTGCAAGTGCAACCACTACAGCAGTGACACCAAAAAACCAAAATGAAGACAAAGAGAAAGAAAAAGCAAGTGATAATAGCTTAGACTTAAATCTTTCTTTGTAA

Protein sequence

>MsG0780041475.01.T01

MEGENRKRKMLEDEEENDDEKMEKFFALIKSTRDILSKTDKKVGEEKKEKCIWNPTFQLEDFIACEELGKSNVSAAAGPSSQQEKEVKIIEKEYVQEVAASATTTAVTPKNQNEDKEKEKASDNSLDLNLSL*