Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007544.01.T01 | XP_003594343.2 | 85.811 | 148 | 2 | 1 | 3 | 150 | 15 | 143 | 1.04E-82 | 252 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007544.01.T01 | Q6DBA5 | 38.255 | 149 | 67 | 5 | 7 | 150 | 19 | 147 | 1.46E-20 | 86.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007544.01.T01 | I3SZT6 | 85.811 | 148 | 2 | 1 | 3 | 150 | 15 | 143 | 4.97e-83 | 252 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001254.01 | MsG0280007544.01 | 0.818264 | 2.146164e-52 | 1.598691e-49 |
| MsG0180001288.01 | MsG0280007544.01 | 0.858771 | 6.553859e-63 | 1.691634e-59 |
| MsG0180001588.01 | MsG0280007544.01 | 0.804641 | 1.959696e-49 | 1.015719e-46 |
| MsG0180005621.01 | MsG0280007544.01 | 0.804618 | 1.981360e-49 | 1.026326e-46 |
| MsG0280006631.01 | MsG0280007544.01 | 0.804134 | 2.498822e-49 | 1.278380e-46 |
| MsG0280006666.01 | MsG0280007544.01 | 0.820147 | 7.997596e-53 | 6.277738e-50 |
| MsG0280007544.01 | MsG0280009980.01 | 0.828599 | 8.221520e-55 | 8.210102e-52 |
| MsG0280007544.01 | MsG0280010234.01 | 0.800244 | 1.578552e-48 | 7.313952e-46 |
| MsG0280007544.01 | MsG0280010825.01 | 0.810469 | 1.134819e-50 | 6.846260e-48 |
| MsG0280007544.01 | MsG0380014544.01 | 0.833096 | 6.490798e-56 | 7.399281e-53 |
| MsG0280007544.01 | MsG0380015340.01 | 0.811332 | 7.382471e-51 | 4.556752e-48 |
| MsG0280007544.01 | MsG0380016819.01 | 0.848441 | 6.117320e-60 | 1.122795e-56 |
| MsG0280007544.01 | MsG0380017416.01 | 0.846723 | 1.815859e-59 | 3.154584e-56 |
| MsG0280007544.01 | MsG0480018875.01 | 0.810602 | 1.062573e-50 | 6.433043e-48 |
| MsG0280007544.01 | MsG0480020937.01 | 0.837282 | 5.697953e-57 | 7.365777e-54 |
| MsG0280007544.01 | MsG0480020997.01 | 0.812516 | 4.076374e-51 | 2.597220e-48 |
| MsG0280007544.01 | MsG0480021486.01 | 0.835185 | 1.944027e-56 | 2.359255e-53 |
| MsG0280007544.01 | MsG0480021740.01 | 0.825316 | 5.010164e-54 | 4.550899e-51 |
| MsG0280007544.01 | MsG0480022378.01 | 0.819254 | 1.279068e-52 | 9.792630e-50 |
| MsG0280007544.01 | MsG0480023626.01 | 0.801176 | 1.018839e-48 | 4.833570e-46 |
| MsG0280007544.01 | MsG0480024019.01 | 0.828826 | 7.245295e-55 | 7.282816e-52 |
| MsG0280007544.01 | MsG0580024276.01 | 0.831057 | 2.070635e-55 | 2.221775e-52 |
| MsG0280007544.01 | MsG0680031356.01 | 0.840680 | 7.514235e-58 | 1.078414e-54 |
| MsG0280007544.01 | MsG0780035917.01 | 0.800534 | 1.377888e-48 | 6.431331e-46 |
| MsG0280007544.01 | MsG0780039274.01 | 0.836321 | 1.002155e-56 | 1.258670e-53 |
| MsG0280007544.01 | MsG0780039536.01 | 0.818515 | 1.883152e-52 | 1.412631e-49 |
| MsG0280007544.01 | MsG0780040459.01 | 0.811248 | 7.699477e-51 | 4.742096e-48 |
| MsG0280007544.01 | MsG0880042392.01 | 0.810486 | 1.125305e-50 | 6.791849e-48 |
| MsG0280007544.01 | MsG0880044344.01 | 0.824770 | 6.739820e-54 | 6.027979e-51 |
| MsG0280007544.01 | MsG0880046529.01 | 0.817676 | 2.914481e-52 | 2.136365e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007544.01.T01 | MTR_2g027530 | 85.811 | 148 | 2 | 1 | 3 | 150 | 15 | 143 | 1.26e-86 | 252 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCATTGCCAAAGTTATTGT+TGG | 0.384552 | 2:+16966599 | MsG0280007544.01.T01:CDS |
| AGAAGTTGGACCGTTTGATC+TGG | 0.404517 | 2:-16966558 | None:intergenic |
| AGAAAACTTCAAATGCTTGA+TGG | 0.417461 | 2:+16967270 | MsG0280007544.01.T01:CDS |
| AGATCAAACGGTCCAACTTC+TGG | 0.429487 | 2:+16966560 | MsG0280007544.01.T01:CDS |
| CAAGGGGACAAGGTTGCAAA+TGG | 0.437247 | 2:+16966743 | MsG0280007544.01.T01:intron |
| ACAAGGAATTCAAGATCAAA+TGG | 0.440815 | 2:+16967315 | MsG0280007544.01.T01:CDS |
| TATTGTTGGCTCAATCGCTT+TGG | 0.451476 | 2:+16966613 | MsG0280007544.01.T01:CDS |
| GATTGAGCCAACAATAACTT+TGG | 0.476308 | 2:-16966606 | None:intergenic |
| TTACCTTTGCCACCAGAAGT+TGG | 0.488935 | 2:-16966572 | None:intergenic |
| TTTGAACATAGAGTTGATCA+AGG | 0.491629 | 2:+16966800 | MsG0280007544.01.T01:CDS |
| TATAATGGTAGGAGGGCTGA+AGG | 0.514957 | 2:+16966325 | None:intergenic |
| TAATGGTAGGAGGGCTGAAG+GGG | 0.541753 | 2:+16966327 | None:intergenic |
| CTGCTGTAGATCATTTGACA+AGG | 0.542544 | 2:+16967487 | MsG0280007544.01.T01:intron |
| CTCTATGTTCAAATTGATGT+GGG | 0.543180 | 2:-16966790 | None:intergenic |
| ATAATGGTAGGAGGGCTGAA+GGG | 0.548492 | 2:+16966326 | None:intergenic |
| GGTCCAACTTCTGGTGGCAA+AGG | 0.550582 | 2:+16966569 | MsG0280007544.01.T01:CDS |
| ACTCTATGTTCAAATTGATG+TGG | 0.554604 | 2:-16966791 | None:intergenic |
| CTATTTGAATCCAGATCAAA+CGG | 0.555560 | 2:+16966548 | MsG0280007544.01.T01:CDS |
| TGCTGTAGATCATTTGACAA+GGG | 0.566891 | 2:+16967488 | MsG0280007544.01.T01:intron |
| GGGGACAAGGTTGCAAATGG+TGG | 0.585394 | 2:+16966746 | MsG0280007544.01.T01:intron |
| AGAGCGTGTCTAACAGACTC+GGG | 0.590587 | 2:-16967554 | None:intergenic |
| CAACAAAATCAGCAAAGGCA+AGG | 0.607041 | 2:+16966363 | MsG0280007544.01.T01:CDS |
| GCTGTAGATCATTTGACAAG+GGG | 0.616908 | 2:+16967489 | MsG0280007544.01.T01:intron |
| AAGAGCGTGTCTAACAGACT+CGG | 0.617302 | 2:-16967555 | None:intergenic |
| GAAGCTGCAGTTATGTTACA+AGG | 0.648952 | 2:+16967298 | MsG0280007544.01.T01:CDS |
| TAGTGCAACAAAATCAGCAA+AGG | 0.649091 | 2:+16966358 | MsG0280007544.01.T01:CDS |
| TCAAACGGTCCAACTTCTGG+TGG | 0.687189 | 2:+16966563 | MsG0280007544.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAATAAAGAAGAAAGTT+AGG | - | Chr2:16967469-16967488 | None:intergenic | 20.0% |
| !! | TATGGTTTACATAAATTCAA+AGG | - | Chr2:16966865-16966884 | None:intergenic | 20.0% |
| !! | TTAAGGTTTGATATTAAAGT+AGG | + | Chr2:16967425-16967444 | MsG0280007544.01.T01:intron | 20.0% |
| !!! | TTTTTTCCTTCAATTTTCAA+GGG | + | Chr2:16966726-16966745 | MsG0280007544.01.T01:intron | 20.0% |
| !!! | TTTTTTTCCTTCAATTTTCA+AGG | + | Chr2:16966725-16966744 | MsG0280007544.01.T01:intron | 20.0% |
| ! | AATTGAAGGAAAAAAAGTTC+AGG | - | Chr2:16966721-16966740 | None:intergenic | 25.0% |
| ! | AGAATTATAATGCTAACCTT+CGG | - | Chr2:16966402-16966421 | None:intergenic | 25.0% |
| ! | ATCAGTAATGTTATCTCTTT+GGG | + | Chr2:16967227-16967246 | MsG0280007544.01.T01:intron | 25.0% |
| ! | ATTATTGCTTGGTTGTATAT+AGG | + | Chr2:16966524-16966543 | MsG0280007544.01.T01:intron | 25.0% |
| ! | CTCTATTGAAAATAATGCAT+GGG | + | Chr2:16967398-16967417 | MsG0280007544.01.T01:intron | 25.0% |
| ! | TATCAATAAGAGATAACTTC+AGG | - | Chr2:16967202-16967221 | None:intergenic | 25.0% |
| ! | TATCAGTAATGTTATCTCTT+TGG | + | Chr2:16967226-16967245 | MsG0280007544.01.T01:intron | 25.0% |
| ! | TCTCTATTGAAAATAATGCA+TGG | + | Chr2:16967397-16967416 | MsG0280007544.01.T01:intron | 25.0% |
| !! | AATAATGCATGGGATTTTTA+AGG | + | Chr2:16967408-16967427 | MsG0280007544.01.T01:intron | 25.0% |
| !!! | CTTTATCTCTCTTTTTAAGT+AGG | + | Chr2:16966962-16966981 | MsG0280007544.01.T01:intron | 25.0% |
| !!! | TATCAATTGCTTTTCATAAG+AGG | - | Chr2:16966930-16966949 | None:intergenic | 25.0% |
| !!! | TATTAATTTGTTCCTCTATG+TGG | + | Chr2:16967131-16967150 | MsG0280007544.01.T01:intron | 25.0% |
| !!! | TTTTTCCTTCAATTTTCAAG+GGG | + | Chr2:16966727-16966746 | MsG0280007544.01.T01:intron | 25.0% |
| AAAATTATTTACCGCCTCAT+TGG | + | Chr2:16967041-16967060 | MsG0280007544.01.T01:intron | 30.0% | |
| AACCACAATAACATTCGATA+AGG | + | Chr2:16967008-16967027 | MsG0280007544.01.T01:intron | 30.0% | |
| ACAAGGAATTCAAGATCAAA+TGG | + | Chr2:16967315-16967334 | MsG0280007544.01.T01:CDS | 30.0% | |
| ACCACAATAACATTCGATAA+GGG | + | Chr2:16967009-16967028 | MsG0280007544.01.T01:intron | 30.0% | |
| ACTCTATGTTCAAATTGATG+TGG | - | Chr2:16966794-16966813 | None:intergenic | 30.0% | |
| AGAAAACTTCAAATGCTTGA+TGG | + | Chr2:16967270-16967289 | MsG0280007544.01.T01:CDS | 30.0% | |
| ATGTTGTGATTTAGCCATTT+AGG | + | Chr2:16967161-16967180 | MsG0280007544.01.T01:intron | 30.0% | |
| CTATTTGAATCCAGATCAAA+CGG | + | Chr2:16966548-16966567 | MsG0280007544.01.T01:CDS | 30.0% | |
| CTCTATGTTCAAATTGATGT+GGG | - | Chr2:16966793-16966812 | None:intergenic | 30.0% | |
| TACAACCAAGCAATAATTTC+AGG | - | Chr2:16966521-16966540 | None:intergenic | 30.0% | |
| ! | TTTGAACATAGAGTTGATCA+AGG | + | Chr2:16966800-16966819 | MsG0280007544.01.T01:CDS | 30.0% |
| !! | ATCAAGCATTTGAAGTTTTC+TGG | - | Chr2:16967271-16967290 | None:intergenic | 30.0% |
| ACCAAGCAATAATTTCAGGA+AGG | - | Chr2:16966517-16966536 | None:intergenic | 35.0% | |
| ACCCTTATCGAATGTTATTG+TGG | - | Chr2:16967013-16967032 | None:intergenic | 35.0% | |
| ACCTTAAACGTCAGACAATA+TGG | - | Chr2:16966898-16966917 | None:intergenic | 35.0% | |
| AGTAATGTTATCTCTTTGGG+TGG | + | Chr2:16967230-16967249 | MsG0280007544.01.T01:intron | 35.0% | |
| CCAAGCAATAATTTCAGGAA+GGG | - | Chr2:16966516-16966535 | None:intergenic | 35.0% | |
| CCTTAAACGTCAGACAATAT+GGG | - | Chr2:16966897-16966916 | None:intergenic | 35.0% | |
| GATTGAGCCAACAATAACTT+TGG | - | Chr2:16966609-16966628 | None:intergenic | 35.0% | |
| GTCATTGCCAAAGTTATTGT+TGG | + | Chr2:16966599-16966618 | MsG0280007544.01.T01:CDS | 35.0% | |
| TAGTGCAACAAAATCAGCAA+AGG | + | Chr2:16966358-16966377 | MsG0280007544.01.T01:CDS | 35.0% | |
| TGCTGTAGATCATTTGACAA+GGG | + | Chr2:16967488-16967507 | MsG0280007544.01.T01:intron | 35.0% | |
| TTCAGGTAATGCAACCTAAA+TGG | - | Chr2:16967178-16967197 | None:intergenic | 35.0% | |
| ! | AACATAGAGTTGATCAAGGT+TGG | + | Chr2:16966804-16966823 | MsG0280007544.01.T01:intron | 35.0% |
| ! | AAGAGATAACTTCAGGTTTC+AGG | - | Chr2:16967195-16967214 | None:intergenic | 35.0% |
| AAACGTCATACAGTACAGGA+TGG | + | Chr2:16967097-16967116 | MsG0280007544.01.T01:intron | 40.0% | |
| ATCTTTGTACTTCTGCTCAG+TGG | + | Chr2:16966425-16966444 | MsG0280007544.01.T01:intron | 40.0% | |
| CAACAAAATCAGCAAAGGCA+AGG | + | Chr2:16966363-16966382 | MsG0280007544.01.T01:CDS | 40.0% | |
| CCCATATTGTCTGACGTTTA+AGG | + | Chr2:16966894-16966913 | MsG0280007544.01.T01:intron | 40.0% | |
| CCCTTCCTGAAATTATTGCT+TGG | + | Chr2:16966513-16966532 | MsG0280007544.01.T01:intron | 40.0% | |
| CTGCTGTAGATCATTTGACA+AGG | + | Chr2:16967487-16967506 | MsG0280007544.01.T01:intron | 40.0% | |
| CTTGTCCCCTTGAAAATTGA+AGG | - | Chr2:16966735-16966754 | None:intergenic | 40.0% | |
| GAAGCTGCAGTTATGTTACA+AGG | + | Chr2:16967298-16967317 | MsG0280007544.01.T01:CDS | 40.0% | |
| GCTAAAACGTCATACAGTAC+AGG | + | Chr2:16967093-16967112 | MsG0280007544.01.T01:intron | 40.0% | |
| GCTGTAGATCATTTGACAAG+GGG | + | Chr2:16967489-16967508 | MsG0280007544.01.T01:intron | 40.0% | |
| TAAACGTCAGACAATATGGG+CGG | - | Chr2:16966894-16966913 | None:intergenic | 40.0% | |
| ! | AGGAAAGTTCAGTTTTCCGA+AGG | + | Chr2:16966383-16966402 | MsG0280007544.01.T01:CDS | 40.0% |
| ! | GTTTTACATCTCCAATGAGG+CGG | - | Chr2:16967055-16967074 | None:intergenic | 40.0% |
| ! | TTGTCTGACGTTTAAGGTTG+TGG | + | Chr2:16966900-16966919 | MsG0280007544.01.T01:intron | 40.0% |
| !! | CTTCAATTTTCAAGGGGACA+AGG | + | Chr2:16966733-16966752 | MsG0280007544.01.T01:intron | 40.0% |
| !! | GTCGTTTTACATCTCCAATG+AGG | - | Chr2:16967058-16967077 | None:intergenic | 40.0% |
| !! | TATTGTTGGCTCAATCGCTT+TGG | + | Chr2:16966613-16966632 | MsG0280007544.01.T01:CDS | 40.0% |
| AACATGAGTCGTCCACATAG+AGG | - | Chr2:16967146-16967165 | None:intergenic | 45.0% | |
| AGAAGTTGGACCGTTTGATC+TGG | - | Chr2:16966561-16966580 | None:intergenic | 45.0% | |
| AGATCAAACGGTCCAACTTC+TGG | + | Chr2:16966560-16966579 | MsG0280007544.01.T01:CDS | 45.0% | |
| CTATCAAAATGCCCGCGTAA+GGG | + | Chr2:16967359-16967378 | MsG0280007544.01.T01:intron | 45.0% | |
| GTCATACAGTACAGGATGGA+GGG | + | Chr2:16967101-16967120 | MsG0280007544.01.T01:intron | 45.0% | |
| TCTATCAAAATGCCCGCGTA+AGG | + | Chr2:16967358-16967377 | MsG0280007544.01.T01:intron | 45.0% | |
| TTACCTTTGCCACCAGAAGT+TGG | - | Chr2:16966575-16966594 | None:intergenic | 45.0% | |
| ! | AAGAGCGTGTCTAACAGACT+CGG | - | Chr2:16967558-16967577 | None:intergenic | 45.0% |
| !! | TACGCGGGCATTTTGATAGA+TGG | - | Chr2:16967358-16967377 | None:intergenic | 45.0% |
| AGAGCGTGTCTAACAGACTC+GGG | - | Chr2:16967557-16967576 | None:intergenic | 50.0% | |
| ATCTCAGTGTCTCCCTTACG+CGG | - | Chr2:16967374-16967393 | None:intergenic | 50.0% | |
| CAAGGGGACAAGGTTGCAAA+TGG | + | Chr2:16966743-16966762 | MsG0280007544.01.T01:intron | 50.0% | |
| CGTCATACAGTACAGGATGG+AGG | + | Chr2:16967100-16967119 | MsG0280007544.01.T01:intron | 50.0% | |
| TCAAACGGTCCAACTTCTGG+TGG | + | Chr2:16966563-16966582 | MsG0280007544.01.T01:CDS | 50.0% | |
| ACGTCAGACAATATGGGCGG+AGG | - | Chr2:16966891-16966910 | None:intergenic | 55.0% | |
| CAATATGGGCGGAGGGAGTA+TGG | - | Chr2:16966883-16966902 | None:intergenic | 55.0% | |
| CGTCAGACAATATGGGCGGA+GGG | - | Chr2:16966890-16966909 | None:intergenic | 55.0% | |
| GGGGACAAGGTTGCAAATGG+TGG | + | Chr2:16966746-16966765 | MsG0280007544.01.T01:intron | 55.0% | |
| GGTCCAACTTCTGGTGGCAA+AGG | + | Chr2:16966569-16966588 | MsG0280007544.01.T01:CDS | 55.0% | |
| TCTCAGTGTCTCCCTTACGC+GGG | - | Chr2:16967373-16967392 | None:intergenic | 55.0% | |
| !!! | CTTGCTGCTGCTGCTGCTTT+TGG | - | Chr2:16967525-16967544 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 16966329 | 16967589 | 16966329 | ID=MsG0280007544.01;Name=MsG0280007544.01 |
| Chr2 | mRNA | 16966329 | 16967589 | 16966329 | ID=MsG0280007544.01.T01;Parent=MsG0280007544.01;Name=MsG0280007544.01.T01;_AED=0.48;_eAED=0.56;_QI=0|0.75|0.4|1|1|1|5|0|152 |
| Chr2 | exon | 16966329 | 16966404 | 16966329 | ID=MsG0280007544.01.T01:exon:3635;Parent=MsG0280007544.01.T01 |
| Chr2 | exon | 16966546 | 16966647 | 16966546 | ID=MsG0280007544.01.T01:exon:3636;Parent=MsG0280007544.01.T01 |
| Chr2 | exon | 16966747 | 16966821 | 16966747 | ID=MsG0280007544.01.T01:exon:3637;Parent=MsG0280007544.01.T01 |
| Chr2 | exon | 16967263 | 16967374 | 16967263 | ID=MsG0280007544.01.T01:exon:3638;Parent=MsG0280007544.01.T01 |
| Chr2 | exon | 16967496 | 16967589 | 16967496 | ID=MsG0280007544.01.T01:exon:3639;Parent=MsG0280007544.01.T01 |
| Chr2 | CDS | 16966329 | 16966404 | 16966329 | ID=MsG0280007544.01.T01:cds;Parent=MsG0280007544.01.T01 |
| Chr2 | CDS | 16966546 | 16966647 | 16966546 | ID=MsG0280007544.01.T01:cds;Parent=MsG0280007544.01.T01 |
| Chr2 | CDS | 16966747 | 16966821 | 16966747 | ID=MsG0280007544.01.T01:cds;Parent=MsG0280007544.01.T01 |
| Chr2 | CDS | 16967263 | 16967374 | 16967263 | ID=MsG0280007544.01.T01:cds;Parent=MsG0280007544.01.T01 |
| Chr2 | CDS | 16967496 | 16967589 | 16967496 | ID=MsG0280007544.01.T01:cds;Parent=MsG0280007544.01.T01 |
| Gene Sequence |
| Protein sequence |
