AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016819.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016819.01.T01 MTR_3g095980 98.876 178 2 0 1 178 1 178 9.75e-130 361
MsG0380016819.01.T01 MTR_4g122740 29.310 174 114 4 5 177 13 178 8.99e-17 74.3
MsG0380016819.01.T01 MTR_2g033630 32.335 167 106 5 12 176 15 176 5.32e-16 72.0
MsG0380016819.01.T01 MTR_4g122890 30.726 179 105 6 7 176 7 175 8.06e-15 68.9
MsG0380016819.01.T01 MTR_8g009300 26.012 173 116 6 13 177 13 181 4.83e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016819.01.T01 AT5G64620 50.000 180 87 1 1 177 1 180 3.14e-63 193
MsG0380016819.01.T01 AT3G17220 27.879 165 114 3 12 176 14 173 2.29e-13 65.1
MsG0380016819.01.T01 AT4G15750 29.197 137 95 1 9 145 8 142 5.13e-13 64.3
MsG0380016819.01.T01 AT5G46930 28.313 166 104 6 5 161 1 160 3.96e-11 59.3

Find 39 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACATAGATTGTAGTATT+TGG 0.248640 3:+88264128 None:intergenic
ATTCAAAAGGGTTCCTGATT+TGG 0.274182 3:-88263809 MsG0380016819.01.T01:CDS
GAGCTTGCCACTAGAGAAAA+TGG 0.275669 3:-88263775 MsG0380016819.01.T01:CDS
GCACACTATGCATTTGTAAA+TGG 0.303461 3:-88264186 MsG0380016819.01.T01:CDS
CCAAGCTTCAGTACAAGATT+TGG 0.336276 3:-88263905 MsG0380016819.01.T01:CDS
AGGCCCTTTGTGTCTGCGTT+TGG 0.336742 3:+88264078 None:intergenic
GGTTGGAGTAAGAGATTTAG+AGG 0.338526 3:+88263996 None:intergenic
CAACAACACTACCTTGAAAA+AGG 0.368143 3:-88263974 MsG0380016819.01.T01:CDS
ACAAGCATTTGGGTAGTCTT+TGG 0.377171 3:+88263840 None:intergenic
ACTTATCTGCACACTCTTTG+AGG 0.396879 3:+88263950 None:intergenic
AATGCATTGTGACAAGCATT+TGG 0.398656 3:+88263829 None:intergenic
CTTGTCACAATGCATTCAAA+AGG 0.421269 3:-88263822 MsG0380016819.01.T01:CDS
TCAGGAGGGTAAACCAAATC+AGG 0.429293 3:+88263796 None:intergenic
GCCTAGCTGTGATCATGGTT+GGG 0.442882 3:-88264059 MsG0380016819.01.T01:CDS
TGCGTTTGGACTGCTAGGAT+TGG 0.446964 3:+88264092 None:intergenic
TTCAAGGTAGTGTTGTTGGT+TGG 0.453115 3:+88263979 None:intergenic
CCAAATCTTGTACTGAAGCT+TGG 0.454754 3:+88263905 None:intergenic
GTGTCTGCGTTTGGACTGCT+AGG 0.461087 3:+88264087 None:intergenic
GGCCTAGCTGTGATCATGGT+TGG 0.485514 3:-88264060 MsG0380016819.01.T01:CDS
CATATATGTGATGTAGCTAT+GGG 0.497869 3:-88263745 MsG0380016819.01.T01:CDS
ATGCATTGTGACAAGCATTT+GGG 0.500606 3:+88263830 None:intergenic
ACATATATGTGATGTAGCTA+TGG 0.505961 3:-88263746 MsG0380016819.01.T01:CDS
GGATGAGAAATAGTGAGAAG+TGG 0.509606 3:+88264217 None:intergenic
TGATAATCTCATCAATATGT+AGG 0.512388 3:-88263716 None:intergenic
TTACAAATGCATAGTGTGCT+AGG 0.521367 3:+88264190 None:intergenic
AAAGGGCCTAGCTGTGATCA+TGG 0.521391 3:-88264064 MsG0380016819.01.T01:CDS
GCTGTGATCATGGTTGGGAT+TGG 0.523662 3:-88264054 MsG0380016819.01.T01:CDS
TCTCTAGTGGCAAGCTCAGG+AGG 0.523727 3:+88263781 None:intergenic
AATCTTGTACTGAAGCTTGG+AGG 0.528434 3:+88263908 None:intergenic
ATGCATAGTGTGCTAGGAAG+AGG 0.536460 3:+88264196 None:intergenic
AGTCCAAACGCAGACACAAA+GGG 0.542036 3:-88264081 MsG0380016819.01.T01:CDS
ATCTTGTACTGAAGCTTGGA+GGG 0.545648 3:+88263909 None:intergenic
AGAGGACAAATATGAAGAAG+TGG 0.553621 3:+88264014 None:intergenic
TTGTCACAATGCATTCAAAA+GGG 0.564017 3:-88263821 MsG0380016819.01.T01:CDS
CTCTAGTGGCAAGCTCAGGA+GGG 0.593107 3:+88263782 None:intergenic
CAGTCCAAACGCAGACACAA+AGG 0.620929 3:-88264082 MsG0380016819.01.T01:CDS
TCCCAACCATGATCACAGCT+AGG 0.662886 3:+88264058 None:intergenic
GCAGATAAGTACAACTATGC+TGG 0.666996 3:-88263937 MsG0380016819.01.T01:CDS
ATATATGTGATGTAGCTATG+GGG 0.736245 3:-88263744 MsG0380016819.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAAACATAGATTGTAGTATT+TGG + Chr3:88263823-88263842 None:intergenic 25.0%
ACATATATGTGATGTAGCTA+TGG - Chr3:88264202-88264221 MsG0380016819.01.T01:CDS 30.0%
ATATATGTGATGTAGCTATG+GGG - Chr3:88264204-88264223 MsG0380016819.01.T01:CDS 30.0%
CATATATGTGATGTAGCTAT+GGG - Chr3:88264203-88264222 MsG0380016819.01.T01:CDS 30.0%
TTGTCACAATGCATTCAAAA+GGG - Chr3:88264127-88264146 MsG0380016819.01.T01:CDS 30.0%
! TTTCAAACCATTTTCTCTAG+TGG + Chr3:88264183-88264202 None:intergenic 30.0%
AATGCATTGTGACAAGCATT+TGG + Chr3:88264122-88264141 None:intergenic 35.0%
AGAGGACAAATATGAAGAAG+TGG + Chr3:88263937-88263956 None:intergenic 35.0%
ATGCATTGTGACAAGCATTT+GGG + Chr3:88264121-88264140 None:intergenic 35.0%
ATTCAAAAGGGTTCCTGATT+TGG - Chr3:88264139-88264158 MsG0380016819.01.T01:CDS 35.0%
CAACAACACTACCTTGAAAA+AGG - Chr3:88263974-88263993 MsG0380016819.01.T01:CDS 35.0%
CTTGTCACAATGCATTCAAA+AGG - Chr3:88264126-88264145 MsG0380016819.01.T01:CDS 35.0%
GCACACTATGCATTTGTAAA+TGG - Chr3:88263762-88263781 MsG0380016819.01.T01:CDS 35.0%
TTACAAATGCATAGTGTGCT+AGG + Chr3:88263761-88263780 None:intergenic 35.0%
!!! CTTTTTCAAGGTAGTGTTGT+TGG + Chr3:88263976-88263995 None:intergenic 35.0%
AATCTTGTACTGAAGCTTGG+AGG + Chr3:88264043-88264062 None:intergenic 40.0%
ACAAGCATTTGGGTAGTCTT+TGG + Chr3:88264111-88264130 None:intergenic 40.0%
ACTTATCTGCACACTCTTTG+AGG + Chr3:88264001-88264020 None:intergenic 40.0%
ATCTTGTACTGAAGCTTGGA+GGG + Chr3:88264042-88264061 None:intergenic 40.0%
CCAAATCTTGTACTGAAGCT+TGG + Chr3:88264046-88264065 None:intergenic 40.0%
CCAAGCTTCAGTACAAGATT+TGG - Chr3:88264043-88264062 MsG0380016819.01.T01:CDS 40.0%
GCAGATAAGTACAACTATGC+TGG - Chr3:88264011-88264030 MsG0380016819.01.T01:CDS 40.0%
GGATGAGAAATAGTGAGAAG+TGG + Chr3:88263734-88263753 None:intergenic 40.0%
GGTTGGAGTAAGAGATTTAG+AGG + Chr3:88263955-88263974 None:intergenic 40.0%
! GCAAGTGCTTTTGATCAAAG+TGG + Chr3:88263793-88263812 None:intergenic 40.0%
! TTCAAGGTAGTGTTGTTGGT+TGG + Chr3:88263972-88263991 None:intergenic 40.0%
!! CTCTTTGAGGACCTTTTTCA+AGG + Chr3:88263988-88264007 None:intergenic 40.0%
AGTCCAAACGCAGACACAAA+GGG - Chr3:88263867-88263886 MsG0380016819.01.T01:CDS 45.0%
ATGCATAGTGTGCTAGGAAG+AGG + Chr3:88263755-88263774 None:intergenic 45.0%
GAGCTTGCCACTAGAGAAAA+TGG - Chr3:88264173-88264192 MsG0380016819.01.T01:CDS 45.0%
TCAGGAGGGTAAACCAAATC+AGG + Chr3:88264155-88264174 None:intergenic 45.0%
! TTTTCTCTAGTGGCAAGCTC+AGG + Chr3:88264173-88264192 None:intergenic 45.0%
AAAGGGCCTAGCTGTGATCA+TGG - Chr3:88263884-88263903 MsG0380016819.01.T01:CDS 50.0%
CAGTCCAAACGCAGACACAA+AGG - Chr3:88263866-88263885 MsG0380016819.01.T01:CDS 50.0%
GCCTAGCTGTGATCATGGTT+GGG - Chr3:88263889-88263908 MsG0380016819.01.T01:CDS 50.0%
GCTGTGATCATGGTTGGGAT+TGG - Chr3:88263894-88263913 MsG0380016819.01.T01:CDS 50.0%
TCCCAACCATGATCACAGCT+AGG + Chr3:88263893-88263912 None:intergenic 50.0%
TGCGTTTGGACTGCTAGGAT+TGG + Chr3:88263859-88263878 None:intergenic 50.0%
AGGCCCTTTGTGTCTGCGTT+TGG + Chr3:88263873-88263892 None:intergenic 55.0%
CTCTAGTGGCAAGCTCAGGA+GGG + Chr3:88264169-88264188 None:intergenic 55.0%
GGCCTAGCTGTGATCATGGT+TGG - Chr3:88263888-88263907 MsG0380016819.01.T01:CDS 55.0%
GTGTCTGCGTTTGGACTGCT+AGG + Chr3:88263864-88263883 None:intergenic 55.0%
TCTCTAGTGGCAAGCTCAGG+AGG + Chr3:88264170-88264189 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 88263717 88264253 88263717 ID=MsG0380016819.01;Name=MsG0380016819.01
Chr3 mRNA 88263717 88264253 88263717 ID=MsG0380016819.01.T01;Parent=MsG0380016819.01;Name=MsG0380016819.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|178
Chr3 exon 88263717 88264253 88263717 ID=MsG0380016819.01.T01:exon:3670;Parent=MsG0380016819.01.T01
Chr3 CDS 88263717 88264253 88263717 ID=MsG0380016819.01.T01:cds;Parent=MsG0380016819.01.T01
Gene Sequence

>MsG0380016819.01.T01

ATGGCTTCTAAGATCCACTTCTCACTATTTCTCATCCTCTTCCTAGCACACTATGCATTTGTAAATGGAGATGCCACTTTGATCAAAAGCACTTGCAAGAACACCAAATACTACAATCTATGTTTCTCTTCTCTCAAATCCAATCCTAGCAGTCCAAACGCAGACACAAAGGGCCTAGCTGTGATCATGGTTGGGATTGGAATGACAAATGCTACTTCCACTTCTTCATATTTGTCCTCTAAATCTCTTACTCCAACCAACAACACTACCTTGAAAAAGGTCCTCAAAGAGTGTGCAGATAAGTACAACTATGCTGGTGATTCCCTCCAAGCTTCAGTACAAGATTTGGCTAATGAAGAATATGATTATGCTTACATACACATCACTGCAGCCAAAGACTACCCAAATGCTTGTCACAATGCATTCAAAAGGGTTCCTGATTTGGTTTACCCTCCTGAGCTTGCCACTAGAGAAAATGGTTTGAAACATATATGTGATGTAGCTATGGGGATCATTGATAATCTCATCAATATGTAG

Protein sequence

>MsG0380016819.01.T01

MASKIHFSLFLILFLAHYAFVNGDATLIKSTCKNTKYYNLCFSSLKSNPSSPNADTKGLAVIMVGIGMTNATSTSSYLSSKSLTPTNNTTLKKVLKECADKYNYAGDSLQASVQDLANEEYDYAYIHITAAKDYPNACHNAFKRVPDLVYPPELATRENGLKHICDVAMGIIDNLINM*