Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008740.01.T01 | KAG2398796.1 | 82.857 | 175 | 26 | 1 | 2 | 172 | 26 | 200 | 1.70E-100 | 298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008740.01.T01 | Q6L4D2 | 46.154 | 143 | 63 | 3 | 25 | 166 | 12 | 141 | 3.33E-26 | 100 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008740.01.T01 | A0A2Z6PI69 | 90.625 | 160 | 15 | 0 | 13 | 172 | 1 | 160 | 1.51e-100 | 295 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000983.01 | MsG0280008740.01 | 0.801294 | 9.635351e-49 | 4.584931e-46 |
| MsG0180003360.01 | MsG0280008740.01 | 0.808900 | 2.467953e-50 | 1.428225e-47 |
| MsG0180005633.01 | MsG0280008740.01 | 0.808303 | 3.308797e-50 | 1.885221e-47 |
| MsG0180005924.01 | MsG0280008740.01 | 0.832899 | 7.264797e-56 | 8.232253e-53 |
| MsG0280008740.01 | MsG0380013636.01 | 0.849940 | 2.341029e-60 | 4.511785e-57 |
| MsG0280008740.01 | MsG0380014594.01 | 0.803387 | 3.571306e-49 | 1.792277e-46 |
| MsG0280008740.01 | MsG0380015190.01 | 0.831600 | 1.522776e-55 | 1.660571e-52 |
| MsG0280008740.01 | MsG0380015736.01 | 0.817803 | 2.728124e-52 | 2.006810e-49 |
| MsG0280008740.01 | MsG0380016480.01 | 0.814833 | 1.259618e-51 | 8.542286e-49 |
| MsG0280008740.01 | MsG0380017421.01 | 0.837850 | 4.076043e-57 | 5.362549e-54 |
| MsG0280008740.01 | MsG0380017779.01 | 0.834621 | 2.695689e-56 | 3.216583e-53 |
| MsG0280008740.01 | MsG0480022036.01 | 0.806509 | 7.948505e-50 | 4.322022e-47 |
| MsG0280008740.01 | MsG0580027461.01 | 0.807045 | 6.121825e-50 | 3.375097e-47 |
| MsG0280008740.01 | MsG0580029298.01 | 0.800022 | 1.751202e-48 | 8.068341e-46 |
| MsG0280008740.01 | MsG0580029494.01 | 0.853592 | 2.158047e-61 | 4.687264e-58 |
| MsG0280008740.01 | MsG0780037371.01 | 0.876947 | 9.305164e-69 | 4.596480e-65 |
| MsG0280008740.01 | MsG0780038655.01 | 0.808144 | 3.578423e-50 | 2.030352e-47 |
| MsG0280008740.01 | MsG0780038707.01 | 0.819469 | 1.142941e-52 | 8.803216e-50 |
| MsG0280008740.01 | MsG0780038952.01 | 0.805314 | 1.417412e-49 | 7.474936e-47 |
| MsG0280008740.01 | MsG0880046940.01 | 0.840886 | 6.637812e-58 | 9.587743e-55 |
| MsG0280008740.01 | MsG0880047228.01 | 0.835518 | 1.601659e-56 | 1.963261e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008740.01.T01 | MTR_2g049340 | 95.625 | 160 | 7 | 0 | 13 | 172 | 1 | 160 | 4.12e-93 | 268 |
| MsG0280008740.01.T01 | MTR_6g068860 | 74.342 | 152 | 39 | 0 | 18 | 169 | 1 | 152 | 2.86e-80 | 235 |
| MsG0280008740.01.T01 | MTR_4g117600 | 48.951 | 143 | 70 | 2 | 33 | 172 | 21 | 163 | 2.61e-39 | 131 |
| MsG0280008740.01.T01 | MTR_1g094035 | 43.662 | 142 | 66 | 3 | 25 | 165 | 12 | 140 | 2.83e-27 | 101 |
| MsG0280008740.01.T01 | MTR_4g117600 | 52.083 | 96 | 46 | 0 | 33 | 128 | 21 | 116 | 7.51e-26 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008740.01.T01 | AT5G46530 | 70.779 | 154 | 45 | 0 | 13 | 166 | 1 | 154 | 1.28e-80 | 236 |
| MsG0280008740.01.T01 | AT1G29520 | 75.000 | 160 | 37 | 1 | 13 | 172 | 1 | 157 | 2.55e-75 | 223 |
| MsG0280008740.01.T01 | AT5G18970 | 44.444 | 153 | 85 | 0 | 13 | 165 | 1 | 153 | 9.72e-40 | 133 |
| MsG0280008740.01.T01 | AT1G04560 | 43.506 | 154 | 73 | 3 | 13 | 165 | 1 | 141 | 9.84e-32 | 113 |
Find 36 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTTATTTGCTTTGATTGC+TGG | 0.240660 | 2:-37509391 | MsG0280008740.01.T01:CDS |
| GCCAATTGCTACTCTTCTTT+TGG | 0.240696 | 2:-37509775 | MsG0280008740.01.T01:CDS |
| GGGATTGGATCATTGATTTC+AGG | 0.284608 | 2:-37509361 | MsG0280008740.01.T01:CDS |
| TTCTACATAGCTGCTATTCA+TGG | 0.313701 | 2:-37508895 | MsG0280008740.01.T01:CDS |
| CTGAAGTCCATGAACGGATA+TGG | 0.332684 | 2:+37509332 | None:intergenic |
| GGCTCTTACTGTTCTTGCTA+TGG | 0.345710 | 2:-37509269 | MsG0280008740.01.T01:CDS |
| ATTTCCCATAGGGAAGTATA+TGG | 0.353145 | 2:+37509432 | None:intergenic |
| TTCATGGTGCTGCTGCTATA+AGG | 0.353684 | 2:-37508879 | MsG0280008740.01.T01:CDS |
| CTTCAGTTGCTGCTATTGCA+TGG | 0.353823 | 2:-37509291 | MsG0280008740.01.T01:CDS |
| ATGATCAATTGCTCTATTCA+TGG | 0.373348 | 2:+37509704 | None:intergenic |
| GCTCTTACTGTTCTTGCTAT+GGG | 0.388278 | 2:-37509268 | MsG0280008740.01.T01:CDS |
| GCTTTGATTGCTGGAGTTGT+TGG | 0.390206 | 2:-37509382 | MsG0280008740.01.T01:CDS |
| CTTTGATTGCTGGAGTTGTT+GGG | 0.394153 | 2:-37509381 | MsG0280008740.01.T01:CDS |
| ATTGCTGGAGTTGTTGGGAT+TGG | 0.414658 | 2:-37509376 | MsG0280008740.01.T01:CDS |
| TTCACCCATATACTTCCCTA+TGG | 0.422542 | 2:-37509437 | MsG0280008740.01.T01:CDS |
| TAACATCAGAAATGCTAGTC+TGG | 0.443721 | 2:-37509054 | MsG0280008740.01.T01:intron |
| AAAACCACAAAACTTAGAAA+TGG | 0.451579 | 2:-37509814 | MsG0280008740.01.T01:CDS |
| GACTCAACCATATCCGTTCA+TGG | 0.451664 | 2:-37509339 | MsG0280008740.01.T01:CDS |
| TTCAGTTGCTGCTATTGCAT+GGG | 0.452736 | 2:-37509290 | MsG0280008740.01.T01:CDS |
| TTGTTATAAGCATTGGTGGA+TGG | 0.461891 | 2:-37509728 | MsG0280008740.01.T01:CDS |
| GCAGCAACTGAAGCTGCAGC+TGG | 0.466603 | 2:+37509301 | None:intergenic |
| TGTTATAAGCATTGGTGGAT+GGG | 0.469274 | 2:-37509727 | MsG0280008740.01.T01:CDS |
| GTCATTGTTATAAGCATTGG+TGG | 0.489752 | 2:-37509732 | MsG0280008740.01.T01:CDS |
| ATGAATAGAGCAATTGATCA+TGG | 0.493796 | 2:-37509702 | MsG0280008740.01.T01:CDS |
| ACCAAAAGAAGAGTAGCAAT+TGG | 0.500043 | 2:+37509774 | None:intergenic |
| AGAAAAGCTGTGTAGCTGTA+AGG | 0.509231 | 2:+37508914 | None:intergenic |
| CCTATGGGAAATGCTGCCAC+TGG | 0.518133 | 2:-37509421 | MsG0280008740.01.T01:CDS |
| TCACCCATATACTTCCCTAT+GGG | 0.537916 | 2:-37509436 | MsG0280008740.01.T01:CDS |
| CTCTTACTGTTCTTGCTATG+GGG | 0.540451 | 2:-37509267 | MsG0280008740.01.T01:intron |
| CAGTGGCAGCATTTCCCATA+GGG | 0.547995 | 2:+37509422 | None:intergenic |
| TATGTCATTGTTATAAGCAT+TGG | 0.550512 | 2:-37509735 | MsG0280008740.01.T01:CDS |
| TGAGCAGGAAGATCAAATCC+TGG | 0.560912 | 2:+37509463 | None:intergenic |
| CCAGTGGCAGCATTTCCCAT+AGG | 0.588951 | 2:+37509421 | None:intergenic |
| TTTCCCATAGGGAAGTATAT+GGG | 0.602197 | 2:+37509433 | None:intergenic |
| AGCTCTCTGAAGTCCATGAA+CGG | 0.627243 | 2:+37509326 | None:intergenic |
| AAATAACACAAAGAATCCAG+TGG | 0.711364 | 2:+37509405 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCAAAACTCTTTGTTAA+GGG | + | Chr2:37509730-37509749 | None:intergenic | 20.0% |
| !!! | AATTGATTTTGATATTTGAG+TGG | - | Chr2:37509106-37509125 | MsG0280008740.01.T01:intron | 20.0% |
| ! | AAAACCACAAAACTTAGAAA+TGG | - | Chr2:37508890-37508909 | MsG0280008740.01.T01:CDS | 25.0% |
| ! | CAATTCAAAACTCTTTGTTA+AGG | + | Chr2:37509731-37509750 | None:intergenic | 25.0% |
| ! | GTAAATTTACTTATTCAGCT+TGG | - | Chr2:37509204-37509223 | MsG0280008740.01.T01:intron | 25.0% |
| ! | TATGTCATTGTTATAAGCAT+TGG | - | Chr2:37508969-37508988 | MsG0280008740.01.T01:intron | 25.0% |
| ! | TTGCATTAATGATATCATCA+AGG | - | Chr2:37509482-37509501 | MsG0280008740.01.T01:intron | 25.0% |
| !! | AAATTTACAAGATGTTGCTT+CGG | + | Chr2:37509192-37509211 | None:intergenic | 25.0% |
| !!! | AATCTTTTCGTTTTGTTTCA+CGG | - | Chr2:37509050-37509069 | MsG0280008740.01.T01:intron | 25.0% |
| !!! | ATTGATCATGGTTTTGTAAT+AGG | - | Chr2:37509014-37509033 | MsG0280008740.01.T01:intron | 25.0% |
| !!! | ATTTCTAAGTTTTGTGGTTT+TGG | + | Chr2:37508891-37508910 | None:intergenic | 25.0% |
| AAATAACACAAAGAATCCAG+TGG | + | Chr2:37509302-37509321 | None:intergenic | 30.0% | |
| ATCATCAAGGTTATAAATCG+AGG | - | Chr2:37509495-37509514 | MsG0280008740.01.T01:intron | 30.0% | |
| ATGAATAGAGCAATTGATCA+TGG | - | Chr2:37509002-37509021 | MsG0280008740.01.T01:intron | 30.0% | |
| ATGATCAATTGCTCTATTCA+TGG | + | Chr2:37509003-37509022 | None:intergenic | 30.0% | |
| GCAAGAACTAAAAGTGATTT+CGG | - | Chr2:37509075-37509094 | MsG0280008740.01.T01:CDS | 30.0% | |
| TTAGTAAGGTCTGCAATATA+AGG | + | Chr2:37509545-37509564 | None:intergenic | 30.0% | |
| ! | AGAATGCCATTTAGTTAGTA+AGG | + | Chr2:37509559-37509578 | None:intergenic | 30.0% |
| ! | GACGGTAAATATATTTTTCC+CGG | + | Chr2:37509681-37509700 | None:intergenic | 30.0% |
| ! | TTCAATTTTGCAGAAAGCTA+TGG | - | Chr2:37509751-37509770 | MsG0280008740.01.T01:CDS | 30.0% |
| !! | ATATGCCACTTTACTAGTTA+GGG | + | Chr2:37509594-37509613 | None:intergenic | 30.0% |
| !! | TTTGCCATTTCTAAGTTTTG+TGG | + | Chr2:37508897-37508916 | None:intergenic | 30.0% |
| !!! | CATATGATTTTTTGCAGCTT+TGG | - | Chr2:37509609-37509628 | MsG0280008740.01.T01:intron | 30.0% |
| AAGGTTATAAATCGAGGTTG+TGG | - | Chr2:37509501-37509520 | MsG0280008740.01.T01:intron | 35.0% | |
| ACCAAAAGAAGAGTAGCAAT+TGG | + | Chr2:37508933-37508952 | None:intergenic | 35.0% | |
| TAACATCAGAAATGCTAGTC+TGG | - | Chr2:37509650-37509669 | MsG0280008740.01.T01:intron | 35.0% | |
| TACGAACATCAGAAAAACGA+CGG | + | Chr2:37509699-37509718 | None:intergenic | 35.0% | |
| TATATGGGTGAAAAATGAGC+AGG | + | Chr2:37509259-37509278 | None:intergenic | 35.0% | |
| TGCAGACCTTACTAACTAAA+TGG | - | Chr2:37509550-37509569 | MsG0280008740.01.T01:intron | 35.0% | |
| TTCTACATAGCTGCTATTCA+TGG | - | Chr2:37509809-37509828 | MsG0280008740.01.T01:CDS | 35.0% | |
| ! | AATTTTGCAGAAAGCTATGG+AGG | - | Chr2:37509754-37509773 | MsG0280008740.01.T01:CDS | 35.0% |
| ! | ATTTCCCATAGGGAAGTATA+TGG | + | Chr2:37509275-37509294 | None:intergenic | 35.0% |
| ! | TTTCCCATAGGGAAGTATAT+GGG | + | Chr2:37509274-37509293 | None:intergenic | 35.0% |
| !! | CATATGCCACTTTACTAGTT+AGG | + | Chr2:37509595-37509614 | None:intergenic | 35.0% |
| !! | GTCATTGTTATAAGCATTGG+TGG | - | Chr2:37508972-37508991 | MsG0280008740.01.T01:intron | 35.0% |
| !! | GTGTTATTTGCTTTGATTGC+TGG | - | Chr2:37509313-37509332 | MsG0280008740.01.T01:CDS | 35.0% |
| !! | TGTTATAAGCATTGGTGGAT+GGG | - | Chr2:37508977-37508996 | MsG0280008740.01.T01:intron | 35.0% |
| !! | TTGTTATAAGCATTGGTGGA+TGG | - | Chr2:37508976-37508995 | MsG0280008740.01.T01:intron | 35.0% |
| AAGGTCTGCAATATAAGGCT+TGG | + | Chr2:37509540-37509559 | None:intergenic | 40.0% | |
| AGAAAAGCTGTGTAGCTGTA+AGG | + | Chr2:37509793-37509812 | None:intergenic | 40.0% | |
| CACATCCCTAACTAGTAAAG+TGG | - | Chr2:37509586-37509605 | MsG0280008740.01.T01:intron | 40.0% | |
| CTTTGATTGCTGGAGTTGTT+GGG | - | Chr2:37509323-37509342 | MsG0280008740.01.T01:CDS | 40.0% | |
| TCACCCATATACTTCCCTAT+GGG | - | Chr2:37509268-37509287 | MsG0280008740.01.T01:CDS | 40.0% | |
| TTCACCCATATACTTCCCTA+TGG | - | Chr2:37509267-37509286 | MsG0280008740.01.T01:intron | 40.0% | |
| TTCAGCTTGGTTATGTTTGC+AGG | - | Chr2:37509217-37509236 | MsG0280008740.01.T01:intron | 40.0% | |
| TTCAGTTGCTGCTATTGCAT+GGG | - | Chr2:37509414-37509433 | MsG0280008740.01.T01:CDS | 40.0% | |
| ! | AAATGCTAGTCTGGTAAGAC+CGG | - | Chr2:37509659-37509678 | MsG0280008740.01.T01:intron | 40.0% |
| ! | CTCTTACTGTTCTTGCTATG+GGG | - | Chr2:37509437-37509456 | MsG0280008740.01.T01:CDS | 40.0% |
| ! | GCCAATTGCTACTCTTCTTT+TGG | - | Chr2:37508929-37508948 | MsG0280008740.01.T01:CDS | 40.0% |
| ! | GCTCTTACTGTTCTTGCTAT+GGG | - | Chr2:37509436-37509455 | MsG0280008740.01.T01:CDS | 40.0% |
| !! | GGGATTGGATCATTGATTTC+AGG | - | Chr2:37509343-37509362 | MsG0280008740.01.T01:CDS | 40.0% |
| ATCGCAGAACCATACAGACT+TGG | - | Chr2:37509165-37509184 | MsG0280008740.01.T01:intron | 45.0% | |
| ATTGCTGGAGTTGTTGGGAT+TGG | - | Chr2:37509328-37509347 | MsG0280008740.01.T01:CDS | 45.0% | |
| CTGAAGTCCATGAACGGATA+TGG | + | Chr2:37509375-37509394 | None:intergenic | 45.0% | |
| CTTCAGTTGCTGCTATTGCA+TGG | - | Chr2:37509413-37509432 | MsG0280008740.01.T01:CDS | 45.0% | |
| GACTCAACCATATCCGTTCA+TGG | - | Chr2:37509365-37509384 | MsG0280008740.01.T01:CDS | 45.0% | |
| GCTTTGATTGCTGGAGTTGT+TGG | - | Chr2:37509322-37509341 | MsG0280008740.01.T01:CDS | 45.0% | |
| GGCTCTTACTGTTCTTGCTA+TGG | - | Chr2:37509435-37509454 | MsG0280008740.01.T01:CDS | 45.0% | |
| TGAGCAGGAAGATCAAATCC+TGG | + | Chr2:37509244-37509263 | None:intergenic | 45.0% | |
| TTGGTTATGTTTGCAGGTCC+AGG | - | Chr2:37509223-37509242 | MsG0280008740.01.T01:intron | 45.0% | |
| ! | AATGCTAGTCTGGTAAGACC+GGG | - | Chr2:37509660-37509679 | MsG0280008740.01.T01:intron | 45.0% |
| !! | AGCTCTCTGAAGTCCATGAA+CGG | + | Chr2:37509381-37509400 | None:intergenic | 45.0% |
| !! | TTCATGGTGCTGCTGCTATA+AGG | - | Chr2:37509825-37509844 | MsG0280008740.01.T01:CDS | 45.0% |
| CAGTGGCAGCATTTCCCATA+GGG | + | Chr2:37509285-37509304 | None:intergenic | 50.0% | |
| TGCTTCGGTCCAAGTCTGTA+TGG | + | Chr2:37509177-37509196 | None:intergenic | 50.0% | |
| CCAGTGGCAGCATTTCCCAT+AGG | + | Chr2:37509286-37509305 | None:intergenic | 55.0% | |
| CCTATGGGAAATGCTGCCAC+TGG | - | Chr2:37509283-37509302 | MsG0280008740.01.T01:CDS | 55.0% | |
| GCAGCAACTGAAGCTGCAGC+TGG | + | Chr2:37509406-37509425 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 37508873 | 37509853 | 37508873 | ID=MsG0280008740.01;Name=MsG0280008740.01 |
| Chr2 | mRNA | 37508873 | 37509853 | 37508873 | ID=MsG0280008740.01.T01;Parent=MsG0280008740.01;Name=MsG0280008740.01.T01;_AED=0.37;_eAED=0.38;_QI=0|0|0|1|1|1|4|0|172 |
| Chr2 | exon | 37509691 | 37509853 | 37509691 | ID=MsG0280008740.01.T01:exon:57889;Parent=MsG0280008740.01.T01 |
| Chr2 | exon | 37509268 | 37509487 | 37509268 | ID=MsG0280008740.01.T01:exon:57888;Parent=MsG0280008740.01.T01 |
| Chr2 | exon | 37509055 | 37509100 | 37509055 | ID=MsG0280008740.01.T01:exon:57887;Parent=MsG0280008740.01.T01 |
| Chr2 | exon | 37508873 | 37508962 | 37508873 | ID=MsG0280008740.01.T01:exon:57886;Parent=MsG0280008740.01.T01 |
| Chr2 | CDS | 37509691 | 37509853 | 37509691 | ID=MsG0280008740.01.T01:cds;Parent=MsG0280008740.01.T01 |
| Chr2 | CDS | 37509268 | 37509487 | 37509268 | ID=MsG0280008740.01.T01:cds;Parent=MsG0280008740.01.T01 |
| Chr2 | CDS | 37509055 | 37509100 | 37509055 | ID=MsG0280008740.01.T01:cds;Parent=MsG0280008740.01.T01 |
| Chr2 | CDS | 37508873 | 37508962 | 37508873 | ID=MsG0280008740.01.T01:cds;Parent=MsG0280008740.01.T01 |
| Gene Sequence |
| Protein sequence |
