AlfalfaGEDB | MsG0280008894.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008894.01

KEGG: -
GO: -
PFAM: PF13639

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008894.01.T01	XP_013464052.1	98.413	189	3	0	1	189	1	189	3.03E-135	386
MsG0280008894.01.T02	XP_013464052.1	84.043	94	7	1	1	86	4	97	4.82E-46	155

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008894.01.T01	Q852U6	62	50	19	0	137	186	198	247	1.46E-15	75.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008894.01.T01	A0A072V8C4	98.413	189	3	0	1	189	1	189	1.45e-135	386
MsG0280008894.01.T02	A0A072V8C4	84.043	94	7	1	1	86	4	97	2.30e-46	155

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047869.01	MsG0280008894.01	0.818883	1.553771e-52	1.177492e-49
MsG0080047944.01	MsG0280008894.01	0.861663	8.756328e-64	2.496097e-60
MsG0080047957.01	MsG0280008894.01	0.858710	6.832452e-63	1.759787e-59
MsG0080047959.01	MsG0280008894.01	0.850267	1.896976e-60	3.695080e-57
MsG0080048231.01	MsG0280008894.01	0.816710	4.807190e-52	3.431575e-49
MsG0080048461.01	MsG0280008894.01	0.819151	1.350220e-52	1.030717e-49
MsG0080048462.01	MsG0280008894.01	0.811952	5.412946e-51	3.396606e-48
MsG0080048463.01	MsG0280008894.01	0.817645	2.962323e-52	2.169526e-49
MsG0080048464.01	MsG0280008894.01	0.832035	1.189175e-55	1.313516e-52
MsG0080048515.01	MsG0280008894.01	0.807337	5.310863e-50	2.950322e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008894.01.T01	MTR_2g461020	98.413	189	3	0	1	189	1	189	3.67e-139	386
MsG0280008894.01.T01	MTR_1g103050	30.729	192	94	5	1	186	1	159	2.28e-18	78.2
MsG0280008894.01.T01	MTR_4g116480	46.809	94	40	2	4	97	1	84	3.40e-16	70.9
MsG0280008894.01.T01	MTR_1g106815	58.333	48	20	0	140	187	198	245	2.41e-14	69.3
MsG0280008894.01.T01	MTR_3g083480	41.429	70	40	1	117	186	213	281	1.50e-13	68.2
MsG0280008894.01.T01	MTR_1g047180	55.319	47	21	0	140	186	681	727	1.09e-12	66.2
MsG0280008894.01.T01	MTR_3g067995	50.000	50	24	1	135	183	205	254	6.34e-12	63.2
MsG0280008894.01.T01	MTR_6g007680	51.923	52	24	1	136	186	60	111	7.35e-12	60.8
MsG0280008894.01.T01	MTR_1g014710	44.898	49	27	0	135	183	226	274	6.73e-11	60.5
MsG0280008894.01.T02	MTR_2g461020	84.043	94	7	1	1	86	4	97	5.84e-50	155

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008894.01.T01	AT2G15580	47.264	201	84	6	1	186	1	194	3.23e-51	163
MsG0280008894.01.T01	AT1G49850	62.000	50	19	0	137	186	198	247	1.49e-16	75.5
MsG0280008894.01.T01	AT2G15580	47.000	100	34	4	1	86	1	95	6.39e-16	70.5
MsG0280008894.01.T01	AT2G15580	47.000	100	34	4	1	86	1	95	8.21e-16	70.9
MsG0280008894.01.T01	AT1G55530	28.962	183	95	5	9	183	109	264	2.48e-13	67.4
MsG0280008894.01.T01	AT1G55530	28.962	183	95	5	9	183	109	264	2.48e-13	67.4
MsG0280008894.01.T01	AT1G04790	50.000	48	24	0	139	186	586	633	5.57e-13	67.0
MsG0280008894.01.T01	AT1G55530	55.102	49	22	0	135	183	183	231	1.72e-12	65.1
MsG0280008894.01.T01	AT3G13430	59.524	42	17	0	142	183	225	266	5.69e-12	63.5
MsG0280008894.01.T01	AT3G13430	59.524	42	17	0	142	183	225	266	5.69e-12	63.5

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Find 51 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGCAGGAATGCTTCC+AGG	0.421917	2:+41128547	MsG0280008894.01.T01:exon
AAAGCATTATTATCTAAAAC+AGG	0.453933	2:+41128460	MsG0280008894.01.T01:five_prime_UTR
AAAGGGTATCAGAAAACATA+AGG	0.481341	2:+41128930	MsG0280008894.01.T01:CDS
AAATGAAAAGCTTGTTTAGG+TGG	0.493240	2:-41128358	None:intergenic
AAATTTGTTGTAGACTTTGT+TGG	0.406680	2:+41129478	MsG0280008894.01.T01:three_prime_UTR
AACTTGTGTGCACAAGGTAA+AGG	0.529312	2:-41129351	None:intergenic
AAGAAATGAAAAGCTTGTTT+AGG	0.331374	2:-41128361	None:intergenic
AAGAACATGAAGTGTGTGGA+AGG	0.571227	2:+41129171	MsG0280008894.01.T01:CDS
AAGGCTTCAAGCATGTGGAA+TGG	0.593453	2:-41129374	None:intergenic
AATGAAAAGCTTGTTTAGGT+GGG	0.491071	2:-41128357	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTTACAAACTGAGGTATAT+GGG	+	Chr2:41129214-41129233	MsG0280008894.01.T01:CDS	30.0%
ATGACAAATTCAGATCACAA+AGG	+	Chr2:41128912-41128931	MsG0280008894.01.T01:CDS	30.0%
GAAAATCTAAAGGGAAAGAA+TGG	-	Chr2:41129439-41129458	None:intergenic	30.0%
GTGTTATGAGAAGAAAAAGA+TGG	-	Chr2:41128628-41128647	None:intergenic	30.0%
TATTTCCTCATCATCTTCAA+TGG	+	Chr2:41128696-41128715	MsG0280008894.01.T01:CDS	30.0%
TGACAAATTCAGATCACAAA+GGG	+	Chr2:41128913-41128932	MsG0280008894.01.T01:CDS	30.0%
TTTGTCATCCAATCTTTGTT+TGG	-	Chr2:41128900-41128919	None:intergenic	30.0%
ACAAACCATTGAAGATGATG+AGG	-	Chr2:41128704-41128723	None:intergenic	35.0%
ATGAGGAAATACGAGATTCA+TGG	-	Chr2:41128687-41128706	None:intergenic	35.0%
CACAGAAAAAAGGACCAAAA+TGG	+	Chr2:41128531-41128550	MsG0280008894.01.T01:exon	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	41128318	41129636	41128318	ID=MsG0280008894.01;Name=MsG0280008894.01
Chr2	mRNA	41128318	41129636	41128318	ID=MsG0280008894.01.T01;Parent=MsG0280008894.01;Name=MsG0280008894.01.T01;_AED=0.17;_eAED=0.17;_QI=232\|1\|1\|1\|0\|0\|3\|184\|189
Chr2	exon	41129167	41129636	41129167	ID=MsG0280008894.01.T01:exon:30030;Parent=MsG0280008894.01.T01
Chr2	exon	41128836	41128951	41128836	ID=MsG0280008894.01.T01:exon:30029;Parent=MsG0280008894.01.T01
Chr2	exon	41128318	41128717	41128318	ID=MsG0280008894.01.T01:exon:30028;Parent=MsG0280008894.01.T01
Chr2	five_prime_UTR	41128318	41128549	41128318	ID=MsG0280008894.01.T01:five_prime_utr;Parent=MsG0280008894.01.T01
Chr2	CDS	41128550	41128717	41128550	ID=MsG0280008894.01.T01:cds;Parent=MsG0280008894.01.T01
Chr2	CDS	41128836	41128951	41128836	ID=MsG0280008894.01.T01:cds;Parent=MsG0280008894.01.T01
Chr2	CDS	41129167	41129452	41129167	ID=MsG0280008894.01.T01:cds;Parent=MsG0280008894.01.T01
Chr2	three_prime_UTR	41129453	41129636	41129453	ID=MsG0280008894.01.T01:three_prime_utr;Parent=MsG0280008894.01.T01
Chr2	mRNA	41128559	41128961	41128559	ID=MsG0280008894.01.T02;Parent=MsG0280008894.01;Name=MsG0280008894.01.T02;_AED=0.41;_eAED=0.42;_QI=0\|0\|0\|1\|0\|0\|2\|0\|86
Chr2	exon	41128822	41128961	41128822	ID=MsG0280008894.01.T02:exon:30032;Parent=MsG0280008894.01.T02
Chr2	exon	41128559	41128679	41128559	ID=MsG0280008894.01.T02:exon:30031;Parent=MsG0280008894.01.T02
Chr2	CDS	41128559	41128679	41128559	ID=MsG0280008894.01.T02:cds;Parent=MsG0280008894.01.T02
Chr2	CDS	41128822	41128961	41128822	ID=MsG0280008894.01.T02:cds;Parent=MsG0280008894.01.T02

Gene Sequence

>MsG0280008894.01.T02

ATGCTTCCAGGAGTTGAATCAGCTAGAAGAAGACGTGTCCATAAGTCTGGAGGATTTTCTGATTCACCATCTTTTTCTTCTCATAACACCTCAAGACGCTCTTCTTTTTGTTTGTATGCAAATTTGAATTTACAGCAAAGAAATATATTATACCAGTCACACCAAGATGAGAATATGGTAGGAGCAGCTAGAGCAGCCAAACAAAGATTGGATGACAAATTCAGATCACAAAGGGTATCAGAAAACATAAGGTATAAATAA

>MsG0280008894.01.T01

ATGGCAGGAATGCTTCCAGGAGTTGAATCAGCTAGAAGAAGACGTGTCCATAAGTCTGGAGGATTTTCTGATTCACCATCTTTTTCTTCTCATAACACCTCAAGACGCTCTTCTTTTTGTTTGTATGCAAGTAACCATGAATCTCGTATTTCCTCATCATCTTCAATGCAAAGAAATATATTATACCAGTCACACCAAGATGAGAATATGGTAGGAGCAGCTAGAGCAGCCAAACAAAGATTGGATGACAAATTCAGATCACAAAGGGTATCAGAAAACATAAGGAAAAAGAACATGAAGTGTGTGGAAGGTAGAAACGAAAGCATAGAAGAGTTACAAACTGAGGTATATGGGACAAAGAAAAGTGGTCCAAGGAAGTTCAGTTGGAGCAAATTGAGCTGGAAAGCATCGGAACAAGAGGATTGTGCTGTGTGTTTGGAATCATTTAAGATTGGAGACAAATTGATTCCTTTACCTTGTGCACACAAGTTCCATTCCACATGCTTGAAGCCTTGGTTAGAAAATAATTCACACTGTCCATGTTGTAGAACTACCATTCTTTCCCTTTAG

Protein sequence

>MsG0280008894.01.T02

MLPGVESARRRRVHKSGGFSDSPSFSSHNTSRRSSFCLYANLNLQQRNILYQSHQDENMVGAARAAKQRLDDKFRSQRVSENIRYK*

>MsG0280008894.01.T01

MAGMLPGVESARRRRVHKSGGFSDSPSFSSHNTSRRSSFCLYASNHESRISSSSSMQRNILYQSHQDENMVGAARAAKQRLDDKFRSQRVSENIRKKNMKCVEGRNESIEELQTEVYGTKKSGPRKFSWSKLSWKASEQEDCAVCLESFKIGDKLIPLPCAHKFHSTCLKPWLENNSHCPCCRTTILSL*