AlfalfaGEDB | MsG0280010258.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010258.01

KEGG: -
GO: GO:0004672 GO:0005524 GO:0006468
PFAM: PF00069

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010258.01.T01	KEH38799.1	68.139	317	45	6	27	289	200	514	5.99E-127	400
MsG0280010258.01.T01	KEH38799.1	59.494	79	13	2	281	358	592	652	8.19E-13	84.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010258.01.T01	Q8RWZ5	41.834	349	111	8	31	289	194	540	3.54E-64	228

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010258.01.T01	A0A072V9T4	68.139	317	45	6	27	289	200	514	2.86e-127	400

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048135.01	MsG0280010258.01	0.802210	6.251026e-49	3.044223e-46
MsG0080048197.01	MsG0280010258.01	0.839138	1.895367e-57	2.593318e-54
MsG0080048687.01	MsG0280010258.01	0.850885	1.271017e-60	2.526438e-57
MsG0080048843.01	MsG0280010258.01	0.814359	1.604195e-51	1.074058e-48
MsG0080048971.01	MsG0280010258.01	0.833093	6.500174e-56	7.409451e-53
MsG0180000339.01	MsG0280010258.01	0.809637	1.714959e-50	1.012313e-47
MsG0180000580.01	MsG0280010258.01	0.823913	1.072290e-53	9.357817e-51
MsG0180000614.01	MsG0280010258.01	0.820333	7.248474e-53	5.718600e-50
MsG0180000615.01	MsG0280010258.01	0.801088	1.062029e-48	5.027539e-46
MsG0180000743.01	MsG0280010258.01	0.807206	5.660314e-50	3.133770e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010258.01.T01	MTR_2g080100	68.139	317	45	6	27	289	200	514	7.26e-131	400
MsG0280010258.01.T01	MTR_2g080100	59.494	79	13	2	281	358	592	652	9.92e-17	84.3
MsG0280010258.01.T01	MTR_2g080090	68.153	314	47	5	27	289	199	510	4.83e-130	397
MsG0280010258.01.T01	MTR_2g073250	59.605	354	62	7	8	289	188	532	1.94e-123	381
MsG0280010258.01.T01	MTR_2g073250	79.487	78	16	0	281	358	610	687	9.20e-32	130
MsG0280010258.01.T01	MTR_3g107070	54.848	361	76	9	8	289	168	520	3.81e-108	342
MsG0280010258.01.T01	MTR_3g107070	75.949	79	19	0	281	359	598	676	4.45e-31	129
MsG0280010258.01.T01	MTR_2g080080	58.383	334	64	8	27	289	199	528	2.13e-106	337
MsG0280010258.01.T01	MTR_2g080080	83.333	78	13	0	281	358	606	683	3.75e-33	135
MsG0280010258.01.T01	MTR_1g012550	38.522	379	126	14	2	289	187	549	1.42e-56	204

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010258.01.T01	AT4G32300	41.834	349	111	8	31	289	194	540	3.61e-65	228
MsG0280010258.01.T01	AT4G32300	74.359	78	20	0	281	358	618	695	4.29e-29	122
MsG0280010258.01.T01	AT5G35370	61.250	80	31	0	281	360	639	718	1.17e-23	106
MsG0280010258.01.T01	AT5G24080	44.275	131	57	4	266	384	505	631	3.04e-22	101
MsG0280010258.01.T01	AT5G24080	44.275	131	57	4	266	384	505	631	3.04e-22	101
MsG0280010258.01.T01	AT5G24080	44.275	131	57	4	266	384	632	758	3.12e-22	101
MsG0280010258.01.T01	AT1G34300	52.500	80	37	1	281	359	608	687	8.82e-20	94.0
MsG0280010258.01.T01	AT1G34300	58.621	58	24	0	232	289	474	531	1.60e-12	70.9
MsG0280010258.01.T01	AT5G20050	55.263	76	34	0	281	356	236	311	5.61e-19	90.1
MsG0280010258.01.T01	AT5G20050	53.448	58	27	0	232	289	93	150	8.01e-12	68.2

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Find 134 sgRNAs with CRISPR-Local

Find 887 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATCTTTATCTCTGGA+TGG	0.569362	2:+66906285	MsG0280010258.01.T01:CDS
AAAAGATCTTTATCTCTGGA+TGG	0.569362	2:+66911683	MsG0280010258.01.T01:CDS
AAAAGCAATGTATACATCAA+TGG	0.437662	2:+66905186	MsG0280010258.01.T01:CDS
AAACAGCCCCTGAACTTGTA+TGG	0.449038	2:-66904344	None:intergenic
AAAGCAATGTATACATCAAT+GGG	0.447034	2:+66905187	MsG0280010258.01.T01:CDS
AAGCGAAAAGGCTGAAGCTG+TGG	0.609947	2:+66910419	MsG0280010258.01.T01:CDS
AAGCTAGAAGGTATTAGTCA+AGG	0.541639	2:+66904732	MsG0280010258.01.T01:CDS
AAGCTAGGTCAAGGAGGTTT+TGG	0.303686	2:+66904660	MsG0280010258.01.T01:CDS
AAGCTGTGGCTGTGCCCAAA+AGG	0.493247	2:+66910433	MsG0280010258.01.T01:CDS
AAGGAAGAATTATGATGTAA+GGG	0.559538	2:+66905236	MsG0280010258.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATAGGCTTAGATGGTT+TGG	+	Chr2:66906548-66906567	MsG0280010258.01.T01:intron	30.0%
AAAAGATCTTTATCTCTGGA+TGG	+	Chr2:66906285-66906304	MsG0280010258.01.T01:CDS	30.0%
AAAATTAACTTTGGCCTGTT+TGG	-	Chr2:66910230-66910249	None:intergenic	30.0%
AAACACTTGTAAGACTGTTT+GGG	-	Chr2:66910898-66910917	None:intergenic	30.0%
AAATAAGCTGGTTATGTTCA+AGG	-	Chr2:66914315-66914334	None:intergenic	30.0%
AACAAACAGATGAGATATAC+CGG	-	Chr2:66911752-66911771	None:intergenic	30.0%
AACAAACATCTCAAAGACAA+CGG	+	Chr2:66916274-66916293	MsG0280010258.01.T01:intron	30.0%
AACAATGCACATAATCTCAA+GGG	+	Chr2:66901992-66902011	MsG0280010258.01.T01:intron	30.0%
AACTAATAACCCGCAATATA+AGG	-	Chr2:66905539-66905558	None:intergenic	30.0%
AACTAGAGAAAATTGTGAAC+AGG	-	Chr2:66912371-66912390	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	66901349	66917113	66901349	ID=MsG0280010258.01;Name=MsG0280010258.01
Chr2	mRNA	66901349	66917113	66901349	ID=MsG0280010258.01.T01;Parent=MsG0280010258.01;Name=MsG0280010258.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|0\|0\|0.66\|1\|1\|12\|174\|559
Chr2	exon	66901349	66901427	66901349	ID=MsG0280010258.01.T01:exon:18336;Parent=MsG0280010258.01.T01
Chr2	exon	66903799	66904113	66903799	ID=MsG0280010258.01.T01:exon:18337;Parent=MsG0280010258.01.T01
Chr2	exon	66904229	66904453	66904229	ID=MsG0280010258.01.T01:exon:18338;Parent=MsG0280010258.01.T01
Chr2	exon	66904541	66904789	66904541	ID=MsG0280010258.01.T01:exon:18339;Parent=MsG0280010258.01.T01
Chr2	exon	66905046	66905266	66905046	ID=MsG0280010258.01.T01:exon:18340;Parent=MsG0280010258.01.T01
Chr2	exon	66906274	66906352	66906274	ID=MsG0280010258.01.T01:exon:18341;Parent=MsG0280010258.01.T01
Chr2	exon	66910395	66910555	66910395	ID=MsG0280010258.01.T01:exon:18342;Parent=MsG0280010258.01.T01
Chr2	exon	66911677	66911751	66911677	ID=MsG0280010258.01.T01:exon:18343;Parent=MsG0280010258.01.T01
Chr2	exon	66913297	66913375	66913297	ID=MsG0280010258.01.T01:exon:18344;Parent=MsG0280010258.01.T01
Chr2	exon	66913794	66913989	66913794	ID=MsG0280010258.01.T01:exon:18345;Parent=MsG0280010258.01.T01
Chr2	exon	66914561	66914621	66914561	ID=MsG0280010258.01.T01:exon:18346;Parent=MsG0280010258.01.T01
Chr2	exon	66917000	66917113	66917000	ID=MsG0280010258.01.T01:exon:18347;Parent=MsG0280010258.01.T01
Chr2	CDS	66901349	66901427	66901349	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66903799	66904113	66903799	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66904229	66904453	66904229	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66904541	66904789	66904541	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66905046	66905266	66905046	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66906274	66906352	66906274	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66910395	66910555	66910395	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66911677	66911751	66911677	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66913297	66913375	66913297	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66913794	66913989	66913794	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	CDS	66914561	66914561	66914561	ID=MsG0280010258.01.T01:cds;Parent=MsG0280010258.01.T01
Chr2	three_prime_UTR	66914562	66914621	66914562	ID=MsG0280010258.01.T01:three_prime_utr;Parent=MsG0280010258.01.T01
Chr2	three_prime_UTR	66917000	66917113	66917000	ID=MsG0280010258.01.T01:three_prime_utr;Parent=MsG0280010258.01.T01

Gene Sequence

>MsG0280010258.01.T01

ATGTTAAGTCACGTAGCGCATGTTAAGTTGCGTAGGGGTAATTTGGTCTTTTACTTTGGGAATGAGCGATATCATTTGGCTGGTTTCAAAACTCCACAGACTTATTGGACTATGCATGATAACCGTAAAATCGTTGATAAGGTCTGCCATGTCGTGGCTTTTGCAAATCTCAGAGACAATTCTTGGAGATTCCATGATAACAGTAGGTCTTTGTTATGGCAATTTATTTTCTCTGTTGATGCAGGTATAAATGCAAGTTGGATTGCAGTTTTGGGAAAAGATGGTGTCATTACTTTCTCTAATCTCAACGGTGGTGGATTAAATGTTGATTCTTCAACAAGAATACCTCAAGATGATTGTGATACACCAGAACCTTGTGATCCCTACAATATTTCTGATGATGGACTTGGTTACTTTGCACTTGATTTTCTTCATCCCTATTTGAATACTGATTTGGCCGGTTGTCAAACATCTTGTCGTGGAAATTGCTCTTGTCTTGCAATGTTCTTCCATACAAGTTCAGGGGCTGTTTCTTGTTGGACAAAGTTTTCAGAATCCGATGATGCTGATTCTGGTTATGTTTCTTACATTAAGGTCTCAAGTCATGGAAGTGGAAGTAGAAAAAGCAAAAGTTACCTGCAGAGAATTCTGAAGAAGACAATTTCTTGGAGATTTAACCGGCATGCCAATCCGTTCCGCTATAAAGATCTTGAAGTTGCAACTAATAACTTCTCTGTGAAGCTAGGTCAAGGAGGTTTTGGATCAGTTTATATAGGAGTTCTACCTGATGGGACTCAATTAGCTGTGAAGAAGCTAGAAGGTATTAGTCAAGGGAAGAAAGAGTTCCGAGCTGAAGTTAGCATCATTGATGATCATTTCATTGCCAAAGTTTCGGATTTTGGTTTAGCCAAGCTTATGAATAGAGAAGAAAGCCATGTTCTTACAACACTAAGAGGAACTTGTGGCTACCTTGCACCCGAGTGGATAACAAACTATGCTATATCAGAGAAAAGCAATGTATACATCAATGGGATGGTGTTGCTAGAGATAATTGCCGGAAGGAAGAATTATGATGTAAGGGTTTATCATTGTAGGAAGGCAAAAGATCTTTATCTCTGGATGGCAAAATGCCCTAATGGCCCGTCTGTTAAATTCTTAGTTAGTGCGGCCAATAACAGGGGAAGAGGAAACGAAGCGAAAAGGCTGAAGCTGTGGCTGTGCCCAAAAGGAAGATGTTGCTCCAGAGAGACAGTGAGGACCCTTTTGGGATGGAAGGATAGAAATGAAAATGAGAATGAAGTCGAGGTTAAGGAGAATGGTGCTTCACCTGAAGGCAAAAGATCTTTATCTCTGGATGGCAAAATGCCCCAACGGTCCCGTCTGTTAAATTTTAGTTTAGTGCCGGTCTTAATATTTAAACATGCATTTGATGCTGGTACACTTGCATTTTTGCGAATGTGGCTTTGCAATGCTATTCTCATTTATTCGCGCTTTGCAGAAGAAGAAAAAGGCTGGAACATTTGCAAAGAAGGTCAAAGCAAAGACAAGGAGGAAATGCATGAGATGGCCAATCCTCTGGAGCCGATGAGTTTGCAGATATGTGGAAAGATTAATCTTAAGTTCACCTGCCTCATTTCACTAAAACTAAGAATGGATATCACCTTATATCGACACGAATGA

Protein sequence

>MsG0280010258.01.T01

MLSHVAHVKLRRGNLVFYFGNERYHLAGFKTPQTYWTMHDNRKIVDKVCHVVAFANLRDNSWRFHDNSRSLLWQFIFSVDAGINASWIAVLGKDGVITFSNLNGGGLNVDSSTRIPQDDCDTPEPCDPYNISDDGLGYFALDFLHPYLNTDLAGCQTSCRGNCSCLAMFFHTSSGAVSCWTKFSESDDADSGYVSYIKVSSHGSGSRKSKSYLQRILKKTISWRFNRHANPFRYKDLEVATNNFSVKLGQGGFGSVYIGVLPDGTQLAVKKLEGISQGKKEFRAEVSIIDDHFIAKVSDFGLAKLMNREESHVLTTLRGTCGYLAPEWITNYAISEKSNVYINGMVLLEIIAGRKNYDVRVYHCRKAKDLYLWMAKCPNGPSVKFLVSAANNRGRGNEAKRLKLWLCPKGRCCSRETVRTLLGWKDRNENENEVEVKENGASPEGKRSLSLDGKMPQRSRLLNFSLVPVLIFKHAFDAGTLAFLRMWLCNAILIYSRFAEEEKGWNICKEGQSKDKEEMHEMANPLEPMSLQICGKINLKFTCLISLKLRMDITLYRHE*