AlfalfaGEDB | MsG0080048971.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048971.01

KEGG: K02975
GO: -
PFAM: PF03297

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048971.01.T01	XP_003596834.1	100	107	0	0	1	107	1	107	7.03E-71	216

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048971.01.T01	Q9SIK2	91.667	108	8	1	1	107	1	108	2.37E-52	162

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048971.01.T01	B7FGM7	100.000	107	0	0	1	107	1	107	3.36e-71	216

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0080048971.01	0.812705	3.706417e-51	2.373211e-48
MsG0080048197.01	MsG0080048971.01	0.807553	4.779505e-50	2.669990e-47
MsG0080048216.01	MsG0080048971.01	0.824999	5.951826e-54	5.357998e-51
MsG0080048687.01	MsG0080048971.01	0.905107	6.082300e-80	9.692493e-76
MsG0080048971.01	MsG0180000139.01	0.861752	8.226545e-64	2.352019e-60
MsG0080048971.01	MsG0180000331.01	0.840370	9.057851e-58	1.287535e-54
MsG0080048971.01	MsG0180000339.01	0.873188	1.784505e-67	7.674847e-64
MsG0080048971.01	MsG0180000575.01	0.877695	5.110880e-69	2.594821e-65
MsG0080048971.01	MsG0180000614.01	0.872651	2.699254e-67	1.138482e-63
MsG0080048971.01	MsG0180000615.01	0.856089	4.070739e-62	9.603994e-59
MsG0080048971.01	MsG0180000743.01	0.831452	1.656141e-55	1.798007e-52
MsG0080048971.01	MsG0180000824.01	0.827486	1.523613e-54	1.472972e-51
MsG0080048971.01	MsG0180001102.01	0.810233	1.276499e-50	7.652820e-48
MsG0080048971.01	MsG0180003117.01	0.843999	9.919627e-59	1.579583e-55
MsG0080048971.01	MsG0180003313.01	0.851775	7.126417e-61	1.457962e-57
MsG0080048971.01	MsG0180003612.01	0.830900	2.262811e-55	2.416798e-52
MsG0080048971.01	MsG0180003651.01	0.861938	7.217398e-64	2.076818e-60
MsG0080048971.01	MsG0180003693.01	0.816296	5.950909e-52	4.200558e-49
MsG0080048971.01	MsG0180003793.01	0.935090	1.463641e-96	1.155258e-91
MsG0080048971.01	MsG0180004009.01	0.873283	1.657433e-67	7.152678e-64
MsG0080048971.01	MsG0180004129.01	0.883001	6.486798e-71	4.040223e-67
MsG0080048971.01	MsG0180004130.01	0.891136	5.229422e-74	4.528277e-70
MsG0080048971.01	MsG0180004398.01	0.806913	6.530520e-50	3.588076e-47
MsG0080048971.01	MsG0180004481.01	0.837944	3.853478e-57	5.084289e-54
MsG0080048971.01	MsG0180004482.01	0.835162	1.969802e-56	2.388885e-53
MsG0080048971.01	MsG0180004509.01	0.866607	2.519382e-65	8.542368e-62
MsG0080048971.01	MsG0180004622.01	0.820486	6.687889e-53	5.298716e-50
MsG0080048971.01	MsG0180004625.01	0.814947	1.188562e-51	8.085038e-49
MsG0080048971.01	MsG0180004854.01	0.907786	3.475503e-81	6.259749e-77
MsG0080048971.01	MsG0180004861.01	0.828172	1.041943e-54	1.027678e-51
MsG0080048971.01	MsG0180004991.01	0.801415	9.101629e-49	4.344031e-46
MsG0080048971.01	MsG0180005334.01	0.805690	1.182033e-49	6.294449e-47
MsG0080048971.01	MsG0180005361.01	0.852729	3.812806e-61	8.042428e-58
MsG0080048971.01	MsG0180005366.01	0.852475	4.506279e-61	9.426624e-58
MsG0080048971.01	MsG0180005426.01	0.906717	1.100750e-80	1.888029e-76
MsG0080048971.01	MsG0180005659.01	0.863200	2.949135e-64	8.867094e-61
MsG0080048971.01	MsG0180005851.01	0.831273	1.832723e-55	1.978995e-52
MsG0080048971.01	MsG0180006013.01	0.831077	2.047909e-55	2.198695e-52
MsG0080048971.01	MsG0280006376.01	0.807889	4.054658e-50	2.284950e-47
MsG0080048971.01	MsG0280006406.01	0.826040	3.373687e-54	3.128310e-51
MsG0080048971.01	MsG0280006422.01	0.800600	1.335541e-48	6.244296e-46
MsG0080048971.01	MsG0280006424.01	0.873961	9.795615e-68	4.331468e-64
MsG0080048971.01	MsG0280006819.01	0.857807	1.268746e-62	3.170045e-59
MsG0080048971.01	MsG0280006836.01	0.869896	2.196232e-66	8.378982e-63
MsG0080048971.01	MsG0280006993.01	0.908611	1.414407e-81	2.644244e-77
MsG0080048971.01	MsG0280007161.01	0.814231	1.711367e-51	1.141897e-48
MsG0080048971.01	MsG0280007196.01	0.800585	1.345051e-48	6.286326e-46
MsG0080048971.01	MsG0280007787.01	0.802265	6.088998e-49	2.969609e-46
MsG0080048971.01	MsG0280007847.01	0.831775	1.378719e-55	1.511450e-52
MsG0080048971.01	MsG0280008118.01	0.915530	5.274024e-85	1.384602e-80
MsG0080048971.01	MsG0280008283.01	0.835294	1.825129e-56	2.222552e-53
MsG0080048971.01	MsG0280009111.01	0.800058	1.721726e-48	7.939930e-46
MsG0080048971.01	MsG0280009141.01	0.834085	3.673949e-56	4.313970e-53
MsG0080048971.01	MsG0280009372.01	0.816812	4.559470e-52	3.263954e-49
MsG0080048971.01	MsG0280009375.01	0.918304	1.837916e-86	5.556144e-82
MsG0080048971.01	MsG0280010002.01	0.855961	4.438843e-62	1.042826e-58
MsG0080048971.01	MsG0280010258.01	0.833093	6.500174e-56	7.409451e-53
MsG0080048971.01	MsG0280010383.01	0.819676	1.024887e-52	7.939161e-50
MsG0080048971.01	MsG0280010495.01	0.889120	3.218692e-73	2.562963e-69
MsG0080048971.01	MsG0280010525.01	0.953843	1.118373e-111	3.019462e-106
MsG0080048971.01	MsG0280010776.01	0.838688	2.479092e-57	3.345949e-54
MsG0080048971.01	MsG0280010801.01	0.903385	3.665752e-79	5.414033e-75
MsG0080048971.01	MsG0280010995.01	0.813979	1.945153e-51	1.288995e-48
MsG0080048971.01	MsG0280011234.01	0.850674	1.457824e-60	2.877642e-57
MsG0080048971.01	MsG0280011388.01	0.838821	2.289902e-57	3.103069e-54
MsG0080048971.01	MsG0380011614.01	0.802988	4.319400e-49	2.145613e-46
MsG0080048971.01	MsG0380012073.01	0.944594	1.463349e-103	2.114848e-98
MsG0080048971.01	MsG0380013641.01	0.807522	4.850827e-50	2.707790e-47
MsG0080048971.01	MsG0380014647.01	0.802253	6.124721e-49	2.986138e-46
MsG0080048971.01	MsG0380015027.01	0.847237	1.313336e-59	2.319342e-56
MsG0080048971.01	MsG0380015435.01	0.809229	2.098126e-50	1.225060e-47
MsG0080048971.01	MsG0380015831.01	0.813598	2.360551e-51	1.548280e-48
MsG0080048971.01	MsG0380016290.01	0.853675	2.042798e-61	4.448700e-58
MsG0080048971.01	MsG0380016389.01	0.888303	6.656835e-73	5.129498e-69
MsG0080048971.01	MsG0380016775.01	0.820582	6.357055e-53	5.049754e-50
MsG0080048971.01	MsG0380016778.01	0.842147	3.087840e-58	4.639031e-55
MsG0080048971.01	MsG0380016860.01	0.804250	2.363838e-49	1.212865e-46
MsG0080048971.01	MsG0380017330.01	0.804568	2.029895e-49	1.050098e-46
MsG0080048971.01	MsG0380017382.01	0.903849	2.265668e-79	3.413100e-75
MsG0080048971.01	MsG0380017556.01	0.849314	3.502364e-60	6.613237e-57
MsG0080048971.01	MsG0480018084.01	0.848856	4.693114e-60	8.730642e-57
MsG0080048971.01	MsG0480018252.01	0.814142	1.791388e-51	1.192382e-48
MsG0080048971.01	MsG0480018477.01	0.917203	7.062972e-86	2.014179e-81
MsG0080048971.01	MsG0480018894.01	0.843233	1.589401e-58	2.470609e-55
MsG0080048971.01	MsG0480020959.01	0.859305	4.535567e-63	1.192340e-59
MsG0080048971.01	MsG0480021006.01	0.888053	8.300680e-73	6.328963e-69
MsG0080048971.01	MsG0480021037.01	0.802920	4.461478e-49	2.212460e-46
MsG0080048971.01	MsG0480021077.01	-0.827734	1.328329e-54	1.293386e-51
MsG0080048971.01	MsG0480021159.01	0.863350	2.651014e-64	8.012665e-61
MsG0080048971.01	MsG0480021448.01	0.837381	5.374187e-57	6.968178e-54
MsG0080048971.01	MsG0480021453.01	0.811326	7.404863e-51	4.569814e-48
MsG0080048971.01	MsG0480021832.01	0.817515	3.168863e-52	2.312633e-49
MsG0080048971.01	MsG0480022223.01	0.812649	3.811357e-51	2.436810e-48
MsG0080048971.01	MsG0480022693.01	0.809403	1.925554e-50	1.129507e-47
MsG0080048971.01	MsG0480022836.01	0.803319	3.688842e-49	1.848026e-46
MsG0080048971.01	MsG0480022913.01	0.830245	3.272965e-55	3.429324e-52
MsG0080048971.01	MsG0480022960.01	0.844650	6.630262e-59	1.077519e-55
MsG0080048971.01	MsG0480023149.01	0.919007	7.704208e-87	2.418513e-82
MsG0080048971.01	MsG0480023221.01	0.831956	1.243975e-55	1.370904e-52
MsG0080048971.01	MsG0480023361.01	0.809641	1.711756e-50	1.010519e-47
MsG0080048971.01	MsG0480023399.01	0.843895	1.057237e-58	1.678281e-55
MsG0080048971.01	MsG0480023467.01	0.890031	1.422911e-73	1.176233e-69
MsG0080048971.01	MsG0480023559.01	0.881389	2.499171e-70	1.461246e-66
MsG0080048971.01	MsG0480023771.01	0.849353	3.415761e-60	6.458160e-57
MsG0080048971.01	MsG0480023968.01	0.805031	1.624291e-49	8.503758e-47
MsG0080048971.01	MsG0580024369.01	0.873977	9.671592e-68	4.279322e-64
MsG0080048971.01	MsG0580025400.01	0.803998	2.667337e-49	1.359881e-46
MsG0080048971.01	MsG0580026157.01	0.858042	1.080983e-62	2.722309e-59
MsG0080048971.01	MsG0580026226.01	0.834424	3.022264e-56	3.585227e-53
MsG0080048971.01	MsG0580027043.01	0.894942	1.532772e-75	1.557137e-71
MsG0080048971.01	MsG0580029127.01	0.830690	2.547547e-55	2.704151e-52
MsG0080048971.01	MsG0580029132.01	0.923764	1.726865e-89	7.035238e-85
MsG0080048971.01	MsG0580029300.01	0.818728	1.684994e-52	1.271374e-49
MsG0080048971.01	MsG0580029370.01	0.820838	5.549686e-53	4.440425e-50
MsG0080048971.01	MsG0680030968.01	0.805434	1.337262e-49	7.074137e-47
MsG0080048971.01	MsG0680031628.01	0.862954	3.515119e-64	1.047997e-60
MsG0080048971.01	MsG0680032770.01	0.883447	4.451493e-71	2.822755e-67
MsG0080048971.01	MsG0680034671.01	0.839802	1.274129e-57	1.779369e-54
MsG0080048971.01	MsG0680035306.01	0.802295	6.002375e-49	2.929706e-46
MsG0080048971.01	MsG0680035848.01	0.882459	1.023829e-70	6.241666e-67
MsG0080048971.01	MsG0680035888.01	0.820530	6.533881e-53	5.182925e-50
MsG0080048971.01	MsG0780036240.01	0.893795	4.505367e-75	4.360333e-71
MsG0080048971.01	MsG0780036547.01	0.837029	6.615459e-57	8.486550e-54
MsG0080048971.01	MsG0780036822.01	0.895910	6.116363e-76	6.467278e-72
MsG0080048971.01	MsG0780037093.01	0.804321	2.285220e-49	1.174704e-46
MsG0080048971.01	MsG0780037240.01	0.836214	1.066878e-56	1.335713e-53
MsG0080048971.01	MsG0780037590.01	-0.807677	4.497029e-50	2.520353e-47
MsG0080048971.01	MsG0780038400.01	0.925823	1.087482e-90	4.954765e-86
MsG0080048971.01	MsG0780038587.01	0.922842	5.807268e-89	2.248514e-84
MsG0080048971.01	MsG0780038645.01	0.809134	2.198235e-50	1.280274e-47
MsG0080048971.01	MsG0780039643.01	0.866807	2.175593e-65	7.429876e-62
MsG0080048971.01	MsG0780039848.01	0.831462	1.646710e-55	1.788293e-52
MsG0080048971.01	MsG0780040338.01	0.871348	7.320294e-67	2.943251e-63
MsG0080048971.01	MsG0780040511.01	0.805900	1.067682e-49	5.716120e-47
MsG0080048971.01	MsG0780040569.01	0.801512	8.694562e-49	4.159950e-46
MsG0080048971.01	MsG0780040718.01	0.811340	7.353769e-51	4.539967e-48
MsG0080048971.01	MsG0780040782.01	0.829572	4.776877e-55	4.907875e-52
MsG0080048971.01	MsG0780040900.01	0.861710	8.470855e-64	2.418249e-60
MsG0080048971.01	MsG0780040926.01	0.804243	2.372058e-49	1.216852e-46
MsG0080048971.01	MsG0780041147.01	0.881423	2.429562e-70	1.422602e-66
MsG0080048971.01	MsG0780041223.01	0.917417	5.443840e-86	1.569629e-81
MsG0080048971.01	MsG0780041224.01	0.868389	6.770079e-66	2.444568e-62
MsG0080048971.01	MsG0780041295.01	0.840186	1.011721e-57	1.429977e-54
MsG0080048971.01	MsG0780041332.01	0.813008	3.180306e-51	2.052766e-48
MsG0080048971.01	MsG0780041462.01	0.864003	1.661226e-64	5.136453e-61
MsG0080048971.01	MsG0780041519.01	0.863955	1.720311e-64	5.310136e-61
MsG0080048971.01	MsG0880041967.01	0.860499	1.979828e-63	5.423046e-60
MsG0080048971.01	MsG0880042125.01	0.853442	2.383224e-61	5.150415e-58
MsG0080048971.01	MsG0880042128.01	0.839182	1.845928e-57	2.529219e-54
MsG0080048971.01	MsG0880042129.01	0.815473	9.081131e-52	6.267470e-49
MsG0080048971.01	MsG0880042581.01	0.808765	2.636491e-50	1.520369e-47
MsG0080048971.01	MsG0880042604.01	0.801472	8.861501e-49	4.235533e-46
MsG0080048971.01	MsG0880042779.01	0.817469	3.244280e-52	2.364836e-49
MsG0080048971.01	MsG0880042991.01	0.853629	2.105544e-61	4.578569e-58
MsG0080048971.01	MsG0880043517.01	0.805463	1.318852e-49	6.982097e-47
MsG0080048971.01	MsG0880043592.01	0.814840	1.255010e-51	8.512721e-49
MsG0080048971.01	MsG0880043770.01	0.846227	2.480107e-59	4.239340e-56
MsG0080048971.01	MsG0880044711.01	0.810166	1.319507e-50	7.897086e-48
MsG0080048971.01	MsG0880045145.01	0.812050	5.153109e-51	3.242058e-48
MsG0080048971.01	MsG0880045656.01	0.808571	2.900510e-50	1.664206e-47
MsG0080048971.01	MsG0880046074.01	0.818988	1.470169e-52	1.117463e-49
MsG0080048971.01	MsG0880046177.01	0.896028	5.462475e-76	5.803767e-72
MsG0080048971.01	MsG0880046444.01	0.906226	1.860621e-80	3.120285e-76
MsG0080048971.01	MsG0880046575.01	0.838388	2.961496e-57	3.960564e-54
MsG0080048971.01	MsG0880046879.01	0.841404	4.850291e-58	7.119839e-55
MsG0080048971.01	MsG0880046933.01	0.880640	4.647210e-70	2.640364e-66
MsG0080048971.01	MsG0880047388.01	0.829361	5.373440e-55	5.487830e-52
MsG0080048971.01	MsG0880047427.01	0.822713	2.044022e-53	1.724334e-50
MsG0080048971.01	MsG0880047428.01	0.820734	5.865674e-53	4.679298e-50
MsG0080048971.01	MsG0880047448.01	0.811850	5.696476e-51	3.564708e-48
MsG0080048971.01	MsG0880047607.01	0.823000	1.753099e-53	1.490962e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048971.01.T01	MTR_2g086610	100.000	107	0	0	1	107	1	107	8.51e-75	216
MsG0080048971.01.T01	MTR_4g070600	99.065	107	1	0	1	107	1	107	2.67e-74	215
MsG0080048971.01.T01	MTR_4g070600	98.131	107	1	1	1	107	1	106	7.53e-72	209
MsG0080048971.01.T01	MTR_6g015615	80.556	72	4	2	2	72	13	75	2.70e-17	70.9
MsG0080048971.01.T01	MTR_3g117040	80.556	72	4	2	2	72	13	75	2.70e-17	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048971.01.T01	AT2G21580	91.667	108	8	1	1	107	1	108	2.41e-53	162
MsG0080048971.01.T01	AT4G39200	88.889	108	11	1	1	107	1	108	9.21e-52	158
MsG0080048971.01.T01	AT2G21580	87.963	108	11	2	1	107	1	107	1.09e-51	158
MsG0080048971.01.T01	AT4G39200	87.037	108	12	2	1	107	1	107	4.24e-50	154
MsG0080048971.01.T01	AT4G34555	88.785	107	11	1	1	106	1	107	9.73e-50	153
MsG0080048971.01.T01	AT2G16360	85.714	105	14	1	2	105	2	106	3.03e-46	144

Find 41 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGAGTGATGAGTTTGTATT+TGG	0.207961	contig578end:-15994	None:intergenic
TGCTAGCCGCAGAGAAGATT+TGG	0.298822	contig578end:-15690	None:intergenic
GAAAGCAAAAGAAGAAGAAA+TGG	0.344636	contig578end:+15889	MsG0080048971.01.T01:CDS
GGGGTTGGTGGTTACCATTA+GGG	0.358777	contig578end:-15729	None:intergenic
CAGTCCTCTCTGATCGTCTC+AGG	0.361688	contig578end:+16018	MsG0080048971.01.T01:CDS
GAAGATTTGGATTTGGAAGA+AGG	0.363786	contig578end:-15677	None:intergenic
CATTATTATGAGGATGTGTT+TGG	0.370583	contig578end:+16285	MsG0080048971.01.T01:three_prime_UTR
CCGCAGAGAAGATTTGGATT+TGG	0.380715	contig578end:-15684	None:intergenic
TGATGGCAAGAGGATTGATT+AGG	0.387796	contig578end:+16162	MsG0080048971.01.T01:CDS
TCATCAAAGCCGGCGAAATC+AGG	0.410202	contig578end:+15861	MsG0080048971.01.T01:CDS
GGTGGTGGTGCTGCCTTCTT+CGG	0.414824	contig578end:-15837	None:intergenic
TCGTGCAATCAGAGATTTGA+TGG	0.416147	contig578end:+16145	MsG0080048971.01.T01:CDS
TGAAACCGCCTATGTATTAG+TGG	0.440250	contig578end:-16225	None:intergenic
AAGATTTGGATTTGGAAGAA+GGG	0.455542	contig578end:-15676	None:intergenic
TCATCAACTTCAAACCCTAA+TGG	0.458152	contig578end:+15715	MsG0080048971.01.T01:exon
CTAATACATAGGCGGTTTCA+AGG	0.473470	contig578end:+16228	MsG0080048971.01.T01:exon
AACATGGTCTTGTTTGATCA+AGG	0.477202	contig578end:+15942	MsG0080048971.01.T01:CDS
CAAAGGTAAACAAAAGGAAA+AGG	0.503611	contig578end:+15914	MsG0080048971.01.T01:CDS
TTCAGTGAGAAGCTTATCAT+AGG	0.513766	contig578end:-15967	None:intergenic
GATTTCGCCGGCTTTGATGA+CGG	0.516878	contig578end:-15858	None:intergenic
GGGTTTGTTCTCAATAACTC+AGG	0.519467	contig578end:-15656	None:intergenic
TTAGAGACAACATTATTATG+AGG	0.522671	contig578end:+16275	MsG0080048971.01.T01:three_prime_UTR
ATGGAGCAAAGGTAAACAAA+AGG	0.525529	contig578end:+15908	MsG0080048971.01.T01:CDS
ACCACCACCGTCATCAAAGC+CGG	0.527384	contig578end:+15851	MsG0080048971.01.T01:CDS
CACTAGAGCCACTAATACAT+AGG	0.531061	contig578end:+16217	MsG0080048971.01.T01:CDS
TTTGTTGTAGGCACCGAAGA+AGG	0.534954	contig578end:+15824	MsG0080048971.01.T01:intron
GGAGTGATGAGTTTGTATTT+GGG	0.540872	contig578end:-15993	None:intergenic
ATCAGAGATTTGATGGCAAG+AGG	0.560553	contig578end:+16152	MsG0080048971.01.T01:CDS
TTTCCTCCTCCTGATTTCGC+CGG	0.569055	contig578end:-15870	None:intergenic
GCCGGCTTTGATGACGGTGG+TGG	0.569126	contig578end:-15852	None:intergenic
AGACGATCAGAGAGGACTGA+AGG	0.570313	contig578end:-16014	None:intergenic
GGCAAGAGGATTGATTAGGT+TGG	0.587429	contig578end:+16166	MsG0080048971.01.T01:CDS
AGTCCTCTCTGATCGTCTCA+GGG	0.592148	contig578end:+16019	MsG0080048971.01.T01:CDS
AAGAAGAAGAAATGGAGCAA+AGG	0.611863	contig578end:+15897	MsG0080048971.01.T01:CDS
AAGCCGGCGAAATCAGGAGG+AGG	0.614520	contig578end:+15867	MsG0080048971.01.T01:CDS
TCAAAGCCGGCGAAATCAGG+AGG	0.640955	contig578end:+15864	MsG0080048971.01.T01:CDS
AAAGGAAAAGGTTAACAACA+TGG	0.683005	contig578end:+15926	MsG0080048971.01.T01:CDS
CCAAATCCAAATCTTCTCTG+CGG	0.707561	contig578end:+15684	MsG0080048971.01.T01:five_prime_UTR
TTCGCCGGCTTTGATGACGG+TGG	0.725481	contig578end:-15855	None:intergenic
TAGAGCCACTAATACATAGG+CGG	0.728751	contig578end:+16220	MsG0080048971.01.T01:exon
ATACCCTGAGACGATCAGAG+AGG	0.757534	contig578end:-16022	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGTAATGTTTTCAGATTAA+TGG	+	contig578end:16110-16129	MsG0080048971.01.T01:intron	20.0%
!	TTAGAGACAACATTATTATG+AGG	+	contig578end:16275-16294	MsG0080048971.01.T01:three_prime_UTR	25.0%
!!!	TTTTCAGATTAATGGATCTT+TGG	+	contig578end:16118-16137	MsG0080048971.01.T01:intron	25.0%
	AAAGGAAAAGGTTAACAACA+TGG	+	contig578end:15926-15945	MsG0080048971.01.T01:CDS	30.0%
	AAGATTTGGATTTGGAAGAA+GGG	-	contig578end:15679-15698	None:intergenic	30.0%
	CAAAGGTAAACAAAAGGAAA+AGG	+	contig578end:15914-15933	MsG0080048971.01.T01:CDS	30.0%
	CATTATTATGAGGATGTGTT+TGG	+	contig578end:16285-16304	MsG0080048971.01.T01:three_prime_UTR	30.0%
	GAAAGCAAAAGAAGAAGAAA+TGG	+	contig578end:15889-15908	MsG0080048971.01.T01:CDS	30.0%
	TAAGGAAAGGAATTTGATGA+TGG	-	contig578end:15760-15779	None:intergenic	30.0%
!!	ACTTCCATTTTCTTTGTTGT+AGG	+	contig578end:15812-15831	MsG0080048971.01.T01:intron	30.0%
!!	AGGAATTTGATGATGGAAAT+TGG	-	contig578end:15753-15772	None:intergenic	30.0%
!!	GAATTTGATGATGGAAATTG+GGG	-	contig578end:15751-15770	None:intergenic	30.0%
!!	GGAATTTGATGATGGAAATT+GGG	-	contig578end:15752-15771	None:intergenic	30.0%
	AAGAAGAAGAAATGGAGCAA+AGG	+	contig578end:15897-15916	MsG0080048971.01.T01:CDS	35.0%
	AGGAGTGATGAGTTTGTATT+TGG	-	contig578end:15997-16016	None:intergenic	35.0%
	ATGGAGCAAAGGTAAACAAA+AGG	+	contig578end:15908-15927	MsG0080048971.01.T01:CDS	35.0%
	CGAGATTTAGATCTAAGGAA+AGG	-	contig578end:15773-15792	None:intergenic	35.0%
	GAAGATTTGGATTTGGAAGA+AGG	-	contig578end:15680-15699	None:intergenic	35.0%
	GGAGTGATGAGTTTGTATTT+GGG	-	contig578end:15996-16015	None:intergenic	35.0%
	TCATCAACTTCAAACCCTAA+TGG	+	contig578end:15715-15734	MsG0080048971.01.T01:exon	35.0%
	TGCAACGAGATTTAGATCTA+AGG	-	contig578end:15778-15797	None:intergenic	35.0%
	TTCAGTGAGAAGCTTATCAT+AGG	-	contig578end:15970-15989	None:intergenic	35.0%
!!	AACATGGTCTTGTTTGATCA+AGG	+	contig578end:15942-15961	MsG0080048971.01.T01:CDS	35.0%
	CACTAGAGCCACTAATACAT+AGG	+	contig578end:16217-16236	MsG0080048971.01.T01:CDS	40.0%
	CCAAATCCAAATCTTCTCTG+CGG	+	contig578end:15684-15703	MsG0080048971.01.T01:five_prime_UTR	40.0%
	CTAATACATAGGCGGTTTCA+AGG	+	contig578end:16228-16247	MsG0080048971.01.T01:exon	40.0%
	GAATTGAATCGAAGCGACAA+AGG	-	contig578end:16073-16092	None:intergenic	40.0%
	GGTGCCTACAACAAAGAAAA+TGG	-	contig578end:15819-15838	None:intergenic	40.0%
	TAGAGCCACTAATACATAGG+CGG	+	contig578end:16220-16239	MsG0080048971.01.T01:exon	40.0%
	TCGTGCAATCAGAGATTTGA+TGG	+	contig578end:16145-16164	MsG0080048971.01.T01:CDS	40.0%
	TGAAACCGCCTATGTATTAG+TGG	-	contig578end:16228-16247	None:intergenic	40.0%
	TGATGGCAAGAGGATTGATT+AGG	+	contig578end:16162-16181	MsG0080048971.01.T01:CDS	40.0%
!	ATCAGAGATTTGATGGCAAG+AGG	+	contig578end:16152-16171	MsG0080048971.01.T01:CDS	40.0%
!!	TTGATGATGGAAATTGGGGT+TGG	-	contig578end:15747-15766	None:intergenic	40.0%
	CCGCAGAGAAGATTTGGATT+TGG	-	contig578end:15687-15706	None:intergenic	45.0%
	GGCAAGAGGATTGATTAGGT+TGG	+	contig578end:16166-16185	MsG0080048971.01.T01:CDS	45.0%
!	ATGATGGAAATTGGGGTTGG+TGG	-	contig578end:15744-15763	None:intergenic	45.0%
!!	TTTGTTGTAGGCACCGAAGA+AGG	+	contig578end:15824-15843	MsG0080048971.01.T01:intron	45.0%
	AGACGATCAGAGAGGACTGA+AGG	-	contig578end:16017-16036	None:intergenic	50.0%
	AGTCCTCTCTGATCGTCTCA+GGG	+	contig578end:16019-16038	MsG0080048971.01.T01:CDS	50.0%
	ATACCCTGAGACGATCAGAG+AGG	-	contig578end:16025-16044	None:intergenic	50.0%
	TCATCAAAGCCGGCGAAATC+AGG	+	contig578end:15861-15880	MsG0080048971.01.T01:CDS	50.0%
	TGCTAGCCGCAGAGAAGATT+TGG	-	contig578end:15693-15712	None:intergenic	50.0%
	TTTCCTCCTCCTGATTTCGC+CGG	-	contig578end:15873-15892	None:intergenic	50.0%
!!	GATTTCGCCGGCTTTGATGA+CGG	-	contig578end:15861-15880	None:intergenic	50.0%
!!	GGGGTTGGTGGTTACCATTA+GGG	-	contig578end:15732-15751	None:intergenic	50.0%
!!	TGGGGTTGGTGGTTACCATT+AGG	-	contig578end:15733-15752	None:intergenic	50.0%
	ACCACCACCGTCATCAAAGC+CGG	+	contig578end:15851-15870	MsG0080048971.01.T01:CDS	55.0%
	CAGTCCTCTCTGATCGTCTC+AGG	+	contig578end:16018-16037	MsG0080048971.01.T01:CDS	55.0%
	TCAAAGCCGGCGAAATCAGG+AGG	+	contig578end:15864-15883	MsG0080048971.01.T01:CDS	55.0%
	AAGCCGGCGAAATCAGGAGG+AGG	+	contig578end:15867-15886	MsG0080048971.01.T01:CDS	60.0%
!!	GGTGGTGGTGCTGCCTTCTT+CGG	-	contig578end:15840-15859	None:intergenic	60.0%
!!	TTCGCCGGCTTTGATGACGG+TGG	-	contig578end:15858-15877	None:intergenic	60.0%
!!	GCCGGCTTTGATGACGGTGG+TGG	-	contig578end:15855-15874	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
contig578end	gene	15659	16410	15659	ID=MsG0080048971.01;Name=MsG0080048971.01
contig578end	mRNA	15659	16410	15659	ID=MsG0080048971.01.T01;Parent=MsG0080048971.01;Name=MsG0080048971.01.T01;_AED=0.50;_eAED=0.50;_QI=75\|1\|1\|1\|1\|0.66\|3\|172\|107
contig578end	exon	15659	15736	15659	ID=MsG0080048971.01.T01:exon:18456;Parent=MsG0080048971.01.T01
contig578end	exon	15834	16040	15834	ID=MsG0080048971.01.T01:exon:18457;Parent=MsG0080048971.01.T01
contig578end	exon	16125	16410	16125	ID=MsG0080048971.01.T01:exon:18458;Parent=MsG0080048971.01.T01
contig578end	five_prime_UTR	15659	15733	15659	ID=MsG0080048971.01.T01:five_prime_utr;Parent=MsG0080048971.01.T01
contig578end	CDS	15734	15736	15734	ID=MsG0080048971.01.T01:cds;Parent=MsG0080048971.01.T01
contig578end	CDS	15834	16040	15834	ID=MsG0080048971.01.T01:cds;Parent=MsG0080048971.01.T01
contig578end	CDS	16125	16238	16125	ID=MsG0080048971.01.T01:cds;Parent=MsG0080048971.01.T01
contig578end	three_prime_UTR	16239	16410	16239	ID=MsG0080048971.01.T01:three_prime_utr;Parent=MsG0080048971.01.T01

Gene Sequence	>MsG0080048971.01.T01 ATGGCACCGAAGAAGGCAGCACCACCACCGTCATCAAAGCCGGCGAAATCAGGAGGAGGAAAGCAAAAGAAGAAGAAATGGAGCAAAGGTAAACAAAAGGAAAAGGTTAACAACATGGTCTTGTTTGATCAAGGTACCTATGATAAGCTTCTCACTGAAGCTCCCAAATACAAACTCATCACTCCTTCAGTCCTCTCTGATCGTCTCAGGATTAATGGATCTTTGGCACGTCGTGCAATCAGAGATTTGATGGCAAGAGGATTGATTAGGTTGGTGTCTGCTCATTCTAGTCAGCAGATTTACACTAGAGCCACTAATACATAG
Protein sequence	>MsG0080048971.01.T01 MAPKKAAPPPSSKPAKSGGGKQKKKKWSKGKQKEKVNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMARGLIRLVSAHSSQQIYTRATNT*