AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010522.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010522.01.T01 MTR_2g086580 87.500 360 34 3 1 357 1 352 0.0 616
MsG0280010522.01.T01 MTR_4g052850 64.026 303 96 4 45 344 64 356 1.40e-129 376
MsG0280010522.01.T01 MTR_7g104900 61.364 308 107 4 51 352 71 372 4.44e-126 371
MsG0280010522.01.T01 MTR_8g026680 62.044 274 94 4 46 316 79 345 4.47e-114 341
MsG0280010522.01.T01 MTR_1g089500 66.525 236 72 2 76 308 66 297 2.21e-107 323
MsG0280010522.01.T01 MTR_7g107560 64.474 228 76 1 54 276 32 259 3.22e-100 303
MsG0280010522.01.T01 MTR_5g064420 65.500 200 57 3 97 296 33 220 4.21e-82 250
MsG0280010522.01.T01 MTR_7g081615 66.667 162 52 1 116 277 14 173 1.24e-71 221
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010522.01.T01 AT5G22400 64.401 309 97 4 48 348 87 390 3.27e-131 384
MsG0280010522.01.T01 AT3G11490 53.276 351 131 6 25 347 19 364 5.69e-116 344
MsG0280010522.01.T01 AT1G08340 62.651 249 86 2 77 322 1 245 2.28e-109 323
MsG0280010522.01.T01 AT2G46710 57.667 300 112 5 15 308 43 333 7.70e-109 327
MsG0280010522.01.T01 AT4G03100 57.554 278 100 3 49 312 39 312 1.34e-106 320

Find 96 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAAGGTTGCAAATTTCTA+AGG 0.198022 2:+70923577 MsG0280010522.01.T01:three_prime_UTR
GTAAGTTTGTTTAAAGTATA+AGG 0.231302 2:+70923561 MsG0280010522.01.T01:three_prime_UTR
ATATTATTTGTTGCGTGTTT+TGG 0.231978 2:-70920262 None:intergenic
TGATAGGTTCAATGGTTTCT+TGG 0.248536 2:+70920799 MsG0280010522.01.T01:CDS
TTCTTGAAAATGCTTATATA+AGG 0.254327 2:+70923024 MsG0280010522.01.T01:CDS
CCTAGCACTAGGAGGCCTTC+TGG 0.256375 2:-70920852 None:intergenic
AAAAGTGTCGGTCGAGAAAA+AGG 0.290122 2:+70923435 MsG0280010522.01.T01:three_prime_UTR
TGTCATGGTGGCAATGAAAA+AGG 0.303033 2:-70920504 None:intergenic
GGAACTTCCTAGCGGCGTTC+TGG 0.311234 2:+70922627 MsG0280010522.01.T01:CDS
GATAGGTTCAATGGTTTCTT+GGG 0.329734 2:+70920800 MsG0280010522.01.T01:CDS
TTGTCTGGCAGGACTAATTA+AGG 0.347959 2:+70921830 MsG0280010522.01.T01:CDS
GAAGGTTCTAAATTCAATCT+TGG 0.352625 2:-70923086 None:intergenic
CAATACAGTCTTCCTCAGTT+TGG 0.364130 2:-70922681 None:intergenic
CTGAAGCTGCACTCTTGGAT+TGG 0.367227 2:+70922728 MsG0280010522.01.T01:CDS
TGCATTAACAGAAGAATTGT+TGG 0.374854 2:-70921572 None:intergenic
GAAGTTGAAGTTGAAGTTGA+AGG 0.390622 2:-70920584 None:intergenic
GTATGTTCAAGAAGGGTTGC+AGG 0.395244 2:+70921604 MsG0280010522.01.T01:CDS
TCCTCAACTGAAAGAGAATC+TGG 0.397060 2:+70923249 MsG0280010522.01.T01:exon
GTTGTTGTGTAACATGTTGT+TGG 0.400032 2:+70920169 MsG0280010522.01.T01:five_prime_UTR
TCATGCTGAAGCTGCACTCT+TGG 0.410731 2:+70922723 MsG0280010522.01.T01:CDS
GCGACATGGCGCACGTTTGT+TGG 0.420819 2:-70920764 None:intergenic
TCAGACCGAGCGAAGTCGGC+TGG 0.422333 2:+70923490 MsG0280010522.01.T01:three_prime_UTR
GAACAGACCTGAGTCATGTT+TGG 0.422768 2:-70922832 None:intergenic
CTAGCACTAGGAGGCCTTCT+GGG 0.423002 2:-70920851 None:intergenic
CAATGTACATCAATGTCTTC+AGG 0.428431 2:-70921810 None:intergenic
TACATCAGCCATTAGATTGA+TGG 0.431052 2:-70922752 None:intergenic
AAGTAGTTCAAATGCTAGAA+AGG 0.438382 2:+70923374 MsG0280010522.01.T01:three_prime_UTR
GGAGGCCTTCTGGGAAAATC+AGG 0.439195 2:-70920842 None:intergenic
GTTAGGAATCAATTGAACTT+AGG 0.440142 2:+70921780 MsG0280010522.01.T01:CDS
TCACCCCAGCCGACTTCGCT+CGG 0.461160 2:-70923495 None:intergenic
GGATTGGGCCATCAATCTAA+TGG 0.467322 2:+70922744 MsG0280010522.01.T01:CDS
TGAAGCTGCACTCTTGGATT+GGG 0.473582 2:+70922729 MsG0280010522.01.T01:CDS
AAATCAGGTTCAAACTCATC+AGG 0.480208 2:-70920827 None:intergenic
AAGTAAAATGAGGTGGAGAA+TGG 0.481632 2:-70920535 None:intergenic
ATAGTCAAGAGGAACATGTT+AGG 0.482181 2:+70921763 MsG0280010522.01.T01:CDS
GCGTACATCAATGCAGTGAA+TGG 0.486250 2:-70922985 None:intergenic
ATACTTTAAACAAACTTACA+AGG 0.487138 2:-70923557 None:intergenic
GCCAGATTCTCTTTCAGTTG+AGG 0.491573 2:-70923250 None:intergenic
GAATCAATTGAACTTAGGAT+TGG 0.495739 2:+70921785 MsG0280010522.01.T01:CDS
TGTTACACAACAACATTGAA+AGG 0.509170 2:-70920160 None:intergenic
AGGACAACAACTGTGATCAA+TGG 0.512952 2:+70923412 MsG0280010522.01.T01:three_prime_UTR
AACATTGAAAGGGAAACAAA+AGG 0.515029 2:-70920149 None:intergenic
AGACATTGATGTACATTGTC+TGG 0.518937 2:+70921815 MsG0280010522.01.T01:CDS
AAGATCAGACCGAGCGAAGT+CGG 0.520724 2:+70923486 MsG0280010522.01.T01:three_prime_UTR
AAGGACGCTACGAGAAAGAA+AGG 0.527098 2:+70923043 MsG0280010522.01.T01:CDS
AGTAGTTCAAATGCTAGAAA+GGG 0.528696 2:+70923375 MsG0280010522.01.T01:three_prime_UTR
GTGGGGAAGAATAACAAGTC+TGG 0.528840 2:+70923345 MsG0280010522.01.T01:three_prime_UTR
CAGACCGAGCGAAGTCGGCT+GGG 0.529498 2:+70923491 MsG0280010522.01.T01:three_prime_UTR
CAAAAGTAACGTGTGCGACA+TGG 0.530420 2:-70920778 None:intergenic
GGAAAATGTGGTTGCGAAGG+AGG 0.535139 2:-70920647 None:intergenic
GTTACACAACAACATTGAAA+GGG 0.536375 2:-70920159 None:intergenic
TTGTTGCCACTACTCTTGAA+AGG 0.537642 2:-70920713 None:intergenic
AATTTGTATATGTAGGTGGA+GGG 0.538632 2:+70921717 MsG0280010522.01.T01:intron
CAATTCTTCTGTTAATGCAA+AGG 0.543052 2:+70921576 MsG0280010522.01.T01:CDS
ACATGGCGCACGTTTGTTGG+AGG 0.545851 2:-70920761 None:intergenic
TTATTTCCTTTCAAGAGTAG+TGG 0.553770 2:+70920707 MsG0280010522.01.T01:CDS
ATAAGGTTGCAAATTTCTAA+GGG 0.554414 2:+70923578 MsG0280010522.01.T01:three_prime_UTR
AGGCATCTGTATGTTCAAGA+AGG 0.558492 2:+70921596 MsG0280010522.01.T01:CDS
ATTGATGTACATTGTCTGGC+AGG 0.558765 2:+70921819 MsG0280010522.01.T01:CDS
AGTGGAAAATGTGGTTGCGA+AGG 0.560047 2:-70920650 None:intergenic
AGACCGAGCGAAGTCGGCTG+GGG 0.562843 2:+70923492 MsG0280010522.01.T01:three_prime_UTR
AAATGAGAAATAGTAAGACT+TGG 0.562992 2:-70920369 None:intergenic
CCAGAAGGCCTCCTAGTGCT+AGG 0.566429 2:+70920852 MsG0280010522.01.T01:CDS
GAAATGACTCGAAAAGTCTA+CGG 0.568493 2:-70923124 None:intergenic
CGGTGTACATTCTCATCAGA+AGG 0.569866 2:-70923104 None:intergenic
CAACAAAGACCTTTGCAACA+TGG 0.570037 2:+70920730 MsG0280010522.01.T01:CDS
ATTTGTATATGTAGGTGGAG+GGG 0.570289 2:+70921718 MsG0280010522.01.T01:intron
GACTTATGTCCATGTTGCAA+AGG 0.574114 2:-70920739 None:intergenic
GAAGTTGAAGTTGAAGGTGT+TGG 0.580577 2:-70920578 None:intergenic
AATGAGTCCAGAACGCCGCT+AGG 0.582282 2:-70922634 None:intergenic
GGCATCTGTATGTTCAAGAA+GGG 0.585739 2:+70921597 MsG0280010522.01.T01:CDS
TGAGAATCTAATTTGCAATG+AGG 0.590817 2:+70923290 MsG0280010522.01.T01:three_prime_UTR
AGAATCAGATTTCATTACAA+TGG 0.592660 2:-70923734 None:intergenic
AAGAGTGCAGCTTCAGCATG+AGG 0.593298 2:-70922721 None:intergenic
CAGTTATCTTATGATTCAAG+AGG 0.593577 2:+70921539 MsG0280010522.01.T01:CDS
TAATGCAGACAATAGTCAAG+AGG 0.607790 2:+70921752 MsG0280010522.01.T01:CDS
GAACGCGCGCAACATTGCCA+TGG 0.618131 2:+70922801 MsG0280010522.01.T01:CDS
TATTACATATACCTAGCACT+AGG 0.623513 2:-70920863 None:intergenic
TGCAGCAGCAGACAATGAAG+AGG 0.625545 2:+70923160 MsG0280010522.01.T01:CDS
TGTGATCAATGGAAAAGTGT+CGG 0.625559 2:+70923423 MsG0280010522.01.T01:three_prime_UTR
AGTTAGAATGAAGAAAAGTG+TGG 0.627548 2:-70920323 None:intergenic
TATATAAGTTAAACAAACAC+AGG 0.627876 2:-70923640 None:intergenic
GTTAGAATGAAGAAAAGTGT+GGG 0.630052 2:-70920322 None:intergenic
TGGCAAGAGAATGAAGAGAG+AGG 0.631862 2:-70920611 None:intergenic
AGTTATCTTATGATTCAAGA+GGG 0.643572 2:+70921540 MsG0280010522.01.T01:CDS
TACATATACCTAGCACTAGG+AGG 0.644832 2:-70920860 None:intergenic
TTTCCTCCGAAAAGAAACGT+GGG 0.647448 2:+70923327 MsG0280010522.01.T01:three_prime_UTR
AAGACTGTATTGAACTAGTG+AGG 0.648835 2:+70922692 MsG0280010522.01.T01:CDS
GATGATGAAGTAAAATGAGG+TGG 0.654198 2:-70920542 None:intergenic
GTTGATGATGAAGTAAAATG+AGG 0.658128 2:-70920545 None:intergenic
GTTTCCTCCGAAAAGAAACG+TGG 0.672738 2:+70923326 MsG0280010522.01.T01:three_prime_UTR
AGAATGGAGAACTTCTGTCA+TGG 0.691349 2:-70920519 None:intergenic
TTCCTCCGAAAAGAAACGTG+GGG 0.693572 2:+70923328 MsG0280010522.01.T01:three_prime_UTR
ATGGAGAACTTCTGTCATGG+TGG 0.693833 2:-70920516 None:intergenic
AGTAATGCAATGCCAAACTG+AGG 0.695720 2:+70922669 MsG0280010522.01.T01:CDS
TGGTTTCGGGAACTTCCTAG+CGG 0.730728 2:+70922619 MsG0280010522.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTACGTTTTTAAAA+AGG + Chr2:70922019-70922038 MsG0280010522.01.T01:intron 10.0%
!!! TATAAAAGCATTAATGAAAA+AGG - Chr2:70922550-70922569 None:intergenic 15.0%
!! AAAGTTGAATTTGTATATGT+AGG + Chr2:70921710-70921729 MsG0280010522.01.T01:intron 20.0%
!! ACCATTTATATGTCATAATT+TGG + Chr2:70921007-70921026 MsG0280010522.01.T01:intron 20.0%
!! AGAGAAAAAGTAAAACAAAA+AGG - Chr2:70921458-70921477 None:intergenic 20.0%
!! AGATCATAAACTTTACATAT+AGG - Chr2:70922418-70922437 None:intergenic 20.0%
!! ATACTTTAAACAAACTTACA+AGG - Chr2:70923560-70923579 None:intergenic 20.0%
!! CATAATTTGGATTGAATTTA+GGG + Chr2:70921020-70921039 MsG0280010522.01.T01:intron 20.0%
!! GATCATAAACTTTACATATA+GGG - Chr2:70922417-70922436 None:intergenic 20.0%
!! TATATAAGTTAAACAAACAC+AGG - Chr2:70923643-70923662 None:intergenic 20.0%
!! TCATAATTTGGATTGAATAA+GGG + Chr2:70921243-70921262 MsG0280010522.01.T01:intron 20.0%
!! TCATAATTTGGATTGAATTT+AGG + Chr2:70921019-70921038 MsG0280010522.01.T01:intron 20.0%
!! TCCAAATTATGACATATAAA+TGG - Chr2:70921011-70921030 None:intergenic 20.0%
!! TTATGACATATAAATGGTAA+AGG - Chr2:70921005-70921024 None:intergenic 20.0%
!! TTATTAACAAAAATAAGCGA+TGG - Chr2:70920937-70920956 None:intergenic 20.0%
!! TTCTTGAAAATGCTTATATA+AGG + Chr2:70923024-70923043 MsG0280010522.01.T01:CDS 20.0%
!!! AATATTTAGGATGGTAAATT+GGG + Chr2:70922254-70922273 MsG0280010522.01.T01:intron 20.0%
!!! ATGTTGAATAGTTTTTCTAT+AGG + Chr2:70921974-70921993 MsG0280010522.01.T01:intron 20.0%
!!! GTAAGTTTGTTTAAAGTATA+AGG + Chr2:70923561-70923580 MsG0280010522.01.T01:three_prime_UTR 20.0%
!!! TATTTTCTGAATTAAGAAAG+TGG - Chr2:70920429-70920448 None:intergenic 20.0%
! AAACTTTACATATAGGGTAA+AGG - Chr2:70922411-70922430 None:intergenic 25.0%
! AAATGAGAAATAGTAAGACT+TGG - Chr2:70920372-70920391 None:intergenic 25.0%
! AGAATCAGATTTCATTACAA+TGG - Chr2:70923737-70923756 None:intergenic 25.0%
! AGTAGTGTGTATATAATTCA+TGG + Chr2:70921156-70921175 MsG0280010522.01.T01:intron 25.0%
! AGTTATCTTATGATTCAAGA+GGG + Chr2:70921540-70921559 MsG0280010522.01.T01:CDS 25.0%
! ATAAGGTTGCAAATTTCTAA+GGG + Chr2:70923578-70923597 MsG0280010522.01.T01:three_prime_UTR 25.0%
! ATATATCCTGTAGTTATGAA+TGG + Chr2:70922283-70922302 MsG0280010522.01.T01:intron 25.0%
! GTCATAATTTGGATTGAATA+AGG + Chr2:70921242-70921261 MsG0280010522.01.T01:intron 25.0%
! TAAAGGATTGAAGAAGTTAA+AGG - Chr2:70922394-70922413 None:intergenic 25.0%
! TACATAACTAAATGAACGAT+TGG + Chr2:70921049-70921068 MsG0280010522.01.T01:intron 25.0%
! TATAAGGTTGCAAATTTCTA+AGG + Chr2:70923577-70923596 MsG0280010522.01.T01:three_prime_UTR 25.0%
! TGCTAACTATTATTAGTTCA+TGG + Chr2:70922891-70922910 MsG0280010522.01.T01:intron 25.0%
! TTTGTCTCATTTATTAGACT+TGG - Chr2:70922169-70922188 None:intergenic 25.0%
!! ATCATGAAGTGAGTATTTTA+AGG - Chr2:70920294-70920313 None:intergenic 25.0%
!! CAATATTTAGGATGGTAAAT+TGG + Chr2:70922253-70922272 MsG0280010522.01.T01:intron 25.0%
!! TGATATTCTTGTACTTTTCA+AGG + Chr2:70922594-70922613 MsG0280010522.01.T01:intron 25.0%
!! TTCAAAAGCTATCTAAGATT+TGG + Chr2:70921331-70921350 MsG0280010522.01.T01:intron 25.0%
!! TTTTCAAAGATGAAAATGCA+TGG - Chr2:70922503-70922522 None:intergenic 25.0%
!!! ATATTATTTGTTGCGTGTTT+TGG - Chr2:70920265-70920284 None:intergenic 25.0%
AAATGTCCATTCATAACTAC+AGG - Chr2:70922292-70922311 None:intergenic 30.0%
AACATTGAAAGGGAAACAAA+AGG - Chr2:70920152-70920171 None:intergenic 30.0%
AAGTAGTTCAAATGCTAGAA+AGG + Chr2:70923374-70923393 MsG0280010522.01.T01:three_prime_UTR 30.0%
AATTTGTATATGTAGGTGGA+GGG + Chr2:70921717-70921736 MsG0280010522.01.T01:intron 30.0%
ACTAGTTCAATCATATTGCT+TGG + Chr2:70921683-70921702 MsG0280010522.01.T01:intron 30.0%
AGTAGTTCAAATGCTAGAAA+GGG + Chr2:70923375-70923394 MsG0280010522.01.T01:three_prime_UTR 30.0%
AGTTAGAATGAAGAAAAGTG+TGG - Chr2:70920326-70920345 None:intergenic 30.0%
ATATTGTGAACTTGAACCTA+AGG - Chr2:70922937-70922956 None:intergenic 30.0%
ATGATTCGTTCGCTAATTAA+AGG - Chr2:70921415-70921434 None:intergenic 30.0%
CAATTCTTCTGTTAATGCAA+AGG + Chr2:70921576-70921595 MsG0280010522.01.T01:CDS 30.0%
CAGTTATCTTATGATTCAAG+AGG + Chr2:70921539-70921558 MsG0280010522.01.T01:CDS 30.0%
GAATCAATTGAACTTAGGAT+TGG + Chr2:70921785-70921804 MsG0280010522.01.T01:CDS 30.0%
GCGATGGAAATAAATAAAAC+TGG - Chr2:70920921-70920940 None:intergenic 30.0%
GTGACAATGCATTACTAAAT+TGG + Chr2:70921265-70921284 MsG0280010522.01.T01:intron 30.0%
GTTACACAACAACATTGAAA+GGG - Chr2:70920162-70920181 None:intergenic 30.0%
GTTAGGAATCAATTGAACTT+AGG + Chr2:70921780-70921799 MsG0280010522.01.T01:CDS 30.0%
GTTGAATTTGTATATGTAGG+TGG + Chr2:70921713-70921732 MsG0280010522.01.T01:intron 30.0%
TATTACATATACCTAGCACT+AGG - Chr2:70920866-70920885 None:intergenic 30.0%
TCTGATAGTACTTATACCTT+AGG + Chr2:70922918-70922937 MsG0280010522.01.T01:intron 30.0%
TGATTCATCATGTAACTGAT+TGG + Chr2:70922109-70922128 MsG0280010522.01.T01:intron 30.0%
TGCATTAACAGAAGAATTGT+TGG - Chr2:70921575-70921594 None:intergenic 30.0%
TGTTACACAACAACATTGAA+AGG - Chr2:70920163-70920182 None:intergenic 30.0%
TGTTTAGTGATTGATCTGAA+TGG + Chr2:70921915-70921934 MsG0280010522.01.T01:intron 30.0%
TTACCATCCTAAATATTGCA+TGG - Chr2:70922251-70922270 None:intergenic 30.0%
TTATTTCCTTTCAAGAGTAG+TGG + Chr2:70920707-70920726 MsG0280010522.01.T01:CDS 30.0%
! AACTATGCTAAGAAAAGCAT+AGG + Chr2:70921369-70921388 MsG0280010522.01.T01:intron 30.0%
! AGGTTCAAGTTCACAATATT+TGG + Chr2:70922938-70922957 MsG0280010522.01.T01:intron 30.0%
! GTTAGAATGAAGAAAAGTGT+GGG - Chr2:70920325-70920344 None:intergenic 30.0%
! TGAGAATCTAATTTGCAATG+AGG + Chr2:70923290-70923309 MsG0280010522.01.T01:three_prime_UTR 30.0%
!! ACATCATTTTCAAGACACAT+TGG + Chr2:70922193-70922212 MsG0280010522.01.T01:intron 30.0%
!! GAAGGTTCTAAATTCAATCT+TGG - Chr2:70923089-70923108 None:intergenic 30.0%
!! GTTGATGATGAAGTAAAATG+AGG - Chr2:70920548-70920567 None:intergenic 30.0%
!!! GTTACTTTTGATAGGTTCAA+TGG + Chr2:70920791-70920810 MsG0280010522.01.T01:CDS 30.0%
AAATCAGGTTCAAACTCATC+AGG - Chr2:70920830-70920849 None:intergenic 35.0%
AAGACTGTATTGAACTAGTG+AGG + Chr2:70922692-70922711 MsG0280010522.01.T01:CDS 35.0%
AAGTAAAATGAGGTGGAGAA+TGG - Chr2:70920538-70920557 None:intergenic 35.0%
ACACCATGCAATATTTAGGA+TGG + Chr2:70922245-70922264 MsG0280010522.01.T01:intron 35.0%
AGACATTGATGTACATTGTC+TGG + Chr2:70921815-70921834 MsG0280010522.01.T01:CDS 35.0%
AGCTACACCATGCAATATTT+AGG + Chr2:70922241-70922260 MsG0280010522.01.T01:intron 35.0%
AGCTCAAAAAGTGGAAAATG+TGG - Chr2:70920662-70920681 None:intergenic 35.0%
ATAGTCAAGAGGAACATGTT+AGG + Chr2:70921763-70921782 MsG0280010522.01.T01:CDS 35.0%
ATTCGTTCGCTAATTAAAGG+CGG - Chr2:70921412-70921431 None:intergenic 35.0%
ATTTGTATATGTAGGTGGAG+GGG + Chr2:70921718-70921737 MsG0280010522.01.T01:intron 35.0%
CAATGTACATCAATGTCTTC+AGG - Chr2:70921813-70921832 None:intergenic 35.0%
GAAATGACTCGAAAAGTCTA+CGG - Chr2:70923127-70923146 None:intergenic 35.0%
GAAGTTGAAGTTGAAGTTGA+AGG - Chr2:70920587-70920606 None:intergenic 35.0%
GAATTTGTATATGTAGGTGG+AGG + Chr2:70921716-70921735 MsG0280010522.01.T01:intron 35.0%
GATAGGTTCAATGGTTTCTT+GGG + Chr2:70920800-70920819 MsG0280010522.01.T01:CDS 35.0%
GATGATGAAGTAAAATGAGG+TGG - Chr2:70920545-70920564 None:intergenic 35.0%
GCATTACTAAATTGGATGCT+TGG + Chr2:70921273-70921292 MsG0280010522.01.T01:intron 35.0%
GTTACTAGTAGCTCAAAAAG+TGG - Chr2:70920671-70920690 None:intergenic 35.0%
GTTGTTGTGTAACATGTTGT+TGG + Chr2:70920169-70920188 MsG0280010522.01.T01:five_prime_UTR 35.0%
TAATGCAGACAATAGTCAAG+AGG + Chr2:70921752-70921771 MsG0280010522.01.T01:CDS 35.0%
TACATCAGCCATTAGATTGA+TGG - Chr2:70922755-70922774 None:intergenic 35.0%
TCATGTAACTGATTGGAGAA+AGG + Chr2:70922116-70922135 MsG0280010522.01.T01:intron 35.0%
TGATAGGTTCAATGGTTTCT+TGG + Chr2:70920799-70920818 MsG0280010522.01.T01:CDS 35.0%
! ACATGTAGTGCAACTGTTTT+TGG + Chr2:70921497-70921516 MsG0280010522.01.T01:intron 35.0%
! GATGATTGCATCTAGTTTCT+CGG + Chr2:70921866-70921885 MsG0280010522.01.T01:intron 35.0%
! GTTTTGTGTGTTAGATAGAG+TGG - Chr2:70920212-70920231 None:intergenic 35.0%
! TGTGATCAATGGAAAAGTGT+CGG + Chr2:70923423-70923442 MsG0280010522.01.T01:three_prime_UTR 35.0%
! TTGTTGTCCTCTTGTCTTTT+TGG - Chr2:70923402-70923421 None:intergenic 35.0%
!! TACTTTTCAAGGCTTGGTTT+CGG + Chr2:70922605-70922624 MsG0280010522.01.T01:intron 35.0%
!! TTCTTGTACTTTTCAAGGCT+TGG + Chr2:70922599-70922618 MsG0280010522.01.T01:intron 35.0%
!! TTTTGAGTATTGTGTTGAGC+TGG - Chr2:70920349-70920368 None:intergenic 35.0%
!!! GGAGTTTTTTTCGAGTGATT+CGG - Chr2:70923232-70923251 None:intergenic 35.0%
AAAGGGTCCAAAAAGACAAG+AGG + Chr2:70923392-70923411 MsG0280010522.01.T01:three_prime_UTR 40.0%
AAGGAGGTTGAAAAATGGCA+TGG - Chr2:70920634-70920653 None:intergenic 40.0%
AGAATGGAGAACTTCTGTCA+TGG - Chr2:70920522-70920541 None:intergenic 40.0%
AGGACAACAACTGTGATCAA+TGG + Chr2:70923412-70923431 MsG0280010522.01.T01:three_prime_UTR 40.0%
AGGCATCTGTATGTTCAAGA+AGG + Chr2:70921596-70921615 MsG0280010522.01.T01:CDS 40.0%
AGTAATGCAATGCCAAACTG+AGG + Chr2:70922669-70922688 MsG0280010522.01.T01:CDS 40.0%
ATTGATGTACATTGTCTGGC+AGG + Chr2:70921819-70921838 MsG0280010522.01.T01:CDS 40.0%
CAACAAAGACCTTTGCAACA+TGG + Chr2:70920730-70920749 MsG0280010522.01.T01:CDS 40.0%
CAATACAGTCTTCCTCAGTT+TGG - Chr2:70922684-70922703 None:intergenic 40.0%
GACTTATGTCCATGTTGCAA+AGG - Chr2:70920742-70920761 None:intergenic 40.0%
GGCATCTGTATGTTCAAGAA+GGG + Chr2:70921597-70921616 MsG0280010522.01.T01:CDS 40.0%
TACATATACCTAGCACTAGG+AGG - Chr2:70920863-70920882 None:intergenic 40.0%
TCCTCAACTGAAAGAGAATC+TGG + Chr2:70923249-70923268 MsG0280010522.01.T01:exon 40.0%
TGTCATGGTGGCAATGAAAA+AGG - Chr2:70920507-70920526 None:intergenic 40.0%
TTGCGAAGGAGGTTGAAAAA+TGG - Chr2:70920639-70920658 None:intergenic 40.0%
TTGTCTGGCAGGACTAATTA+AGG + Chr2:70921830-70921849 MsG0280010522.01.T01:CDS 40.0%
TTGTTGCCACTACTCTTGAA+AGG - Chr2:70920716-70920735 None:intergenic 40.0%
TTTCCTCCGAAAAGAAACGT+GGG + Chr2:70923327-70923346 MsG0280010522.01.T01:three_prime_UTR 40.0%
! GAAGTTGAAGTTGAAGGTGT+TGG - Chr2:70920581-70920600 None:intergenic 40.0%
! TTCTTCCCCACGTTTCTTTT+CGG - Chr2:70923336-70923355 None:intergenic 40.0%
! TTGAACCTGATTTTCCCAGA+AGG + Chr2:70920837-70920856 MsG0280010522.01.T01:CDS 40.0%
!! AAAAGTGTCGGTCGAGAAAA+AGG + Chr2:70923435-70923454 MsG0280010522.01.T01:three_prime_UTR 40.0%
!! AAAGTGTCGGTCGAGAAAAA+GGG + Chr2:70923436-70923455 MsG0280010522.01.T01:three_prime_UTR 40.0%
!! ACTTTTCAAGGCTTGGTTTC+GGG + Chr2:70922606-70922625 MsG0280010522.01.T01:intron 40.0%
!! TTGGCTTTTCTGATTGCAGA+TGG + Chr2:70922957-70922976 MsG0280010522.01.T01:intron 40.0%
!!! GTTCGTTTTTTCTCCTTCAC+CGG + Chr2:70922353-70922372 MsG0280010522.01.T01:intron 40.0%
!!! TCGCACACGTTACTTTTGAT+AGG + Chr2:70920783-70920802 MsG0280010522.01.T01:CDS 40.0%
AAGGACCAGTGTTCAAACTC+CGG - Chr2:70922375-70922394 None:intergenic 45.0%
AAGGACGCTACGAGAAAGAA+AGG + Chr2:70923043-70923062 MsG0280010522.01.T01:CDS 45.0%
AGTGGAAAATGTGGTTGCGA+AGG - Chr2:70920653-70920672 None:intergenic 45.0%
ATGGAGAACTTCTGTCATGG+TGG - Chr2:70920519-70920538 None:intergenic 45.0%
CAAAAGTAACGTGTGCGACA+TGG - Chr2:70920781-70920800 None:intergenic 45.0%
CATGTTTGGCGCAAAAACCA+TGG - Chr2:70922821-70922840 None:intergenic 45.0%
CGGTGTACATTCTCATCAGA+AGG - Chr2:70923107-70923126 None:intergenic 45.0%
GAACAGACCTGAGTCATGTT+TGG - Chr2:70922835-70922854 None:intergenic 45.0%
GCCAGATTCTCTTTCAGTTG+AGG - Chr2:70923253-70923272 None:intergenic 45.0%
GCGTACATCAATGCAGTGAA+TGG - Chr2:70922988-70923007 None:intergenic 45.0%
GGATTGGGCCATCAATCTAA+TGG + Chr2:70922744-70922763 MsG0280010522.01.T01:CDS 45.0%
GTATGTTCAAGAAGGGTTGC+AGG + Chr2:70921604-70921623 MsG0280010522.01.T01:CDS 45.0%
GTGGGGAAGAATAACAAGTC+TGG + Chr2:70923345-70923364 MsG0280010522.01.T01:three_prime_UTR 45.0%
GTTTCCTCCGAAAAGAAACG+TGG + Chr2:70923326-70923345 MsG0280010522.01.T01:three_prime_UTR 45.0%
TGAAGCTGCACTCTTGGATT+GGG + Chr2:70922729-70922748 MsG0280010522.01.T01:CDS 45.0%
TGGCAAGAGAATGAAGAGAG+AGG - Chr2:70920614-70920633 None:intergenic 45.0%
TTCCTCCGAAAAGAAACGTG+GGG + Chr2:70923328-70923347 MsG0280010522.01.T01:three_prime_UTR 45.0%
! TTTTGCGCCAAACATGACTC+AGG + Chr2:70922825-70922844 MsG0280010522.01.T01:CDS 45.0%
!! AAAATAAAAAAAAGTTTAAT+TGG - Chr2:70922857-70922876 None:intergenic 5.0%
!! AAAATATATAAAAAAAAGAA+AGG - Chr2:70920464-70920483 None:intergenic 5.0%
!!! AATTAAACTTTTTTTTATTT+TGG + Chr2:70922856-70922875 MsG0280010522.01.T01:intron 5.0%
AAGATCAGACCGAGCGAAGT+CGG + Chr2:70923486-70923505 MsG0280010522.01.T01:three_prime_UTR 50.0%
CAGTGTTCAAACTCCGGTGA+AGG - Chr2:70922369-70922388 None:intergenic 50.0%
CGGTCGAGAAAAAGGGAATG+AGG + Chr2:70923443-70923462 MsG0280010522.01.T01:three_prime_UTR 50.0%
CTGAAGCTGCACTCTTGGAT+TGG + Chr2:70922728-70922747 MsG0280010522.01.T01:CDS 50.0%
CTTCACCGGAGTTTGAACAC+TGG + Chr2:70922367-70922386 MsG0280010522.01.T01:intron 50.0%
GGAAAATGTGGTTGCGAAGG+AGG - Chr2:70920650-70920669 None:intergenic 50.0%
TCATGCTGAAGCTGCACTCT+TGG + Chr2:70922723-70922742 MsG0280010522.01.T01:CDS 50.0%
TGCAGCAGCAGACAATGAAG+AGG + Chr2:70923160-70923179 MsG0280010522.01.T01:CDS 50.0%
TGGTTTCGGGAACTTCCTAG+CGG + Chr2:70922619-70922638 MsG0280010522.01.T01:CDS 50.0%
! AAGAGTGCAGCTTCAGCATG+AGG - Chr2:70922724-70922743 None:intergenic 50.0%
! TTCCCCACGTTTCTTTTCGG+AGG - Chr2:70923333-70923352 None:intergenic 50.0%
AATGAGTCCAGAACGCCGCT+AGG - Chr2:70922637-70922656 None:intergenic 55.0%
CTAGCACTAGGAGGCCTTCT+GGG - Chr2:70920854-70920873 None:intergenic 55.0%
! ACATGGCGCACGTTTGTTGG+AGG - Chr2:70920764-70920783 None:intergenic 55.0%
! GGAGGCCTTCTGGGAAAATC+AGG - Chr2:70920845-70920864 None:intergenic 55.0%
CCAGAAGGCCTCCTAGTGCT+AGG + Chr2:70920852-70920871 MsG0280010522.01.T01:CDS 60.0%
CCTAGCACTAGGAGGCCTTC+TGG - Chr2:70920855-70920874 None:intergenic 60.0%
GAACGCGCGCAACATTGCCA+TGG + Chr2:70922801-70922820 MsG0280010522.01.T01:CDS 60.0%
GCGACATGGCGCACGTTTGT+TGG - Chr2:70920767-70920786 None:intergenic 60.0%
GGAACTTCCTAGCGGCGTTC+TGG + Chr2:70922627-70922646 MsG0280010522.01.T01:CDS 60.0%
AGACCGAGCGAAGTCGGCTG+GGG + Chr2:70923492-70923511 MsG0280010522.01.T01:three_prime_UTR 65.0%
CAGACCGAGCGAAGTCGGCT+GGG + Chr2:70923491-70923510 MsG0280010522.01.T01:three_prime_UTR 65.0%
TCAGACCGAGCGAAGTCGGC+TGG + Chr2:70923490-70923509 MsG0280010522.01.T01:three_prime_UTR 65.0%
! TCACCCCAGCCGACTTCGCT+CGG - Chr2:70923498-70923517 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 70920137 70923760 70920137 ID=MsG0280010522.01;Name=MsG0280010522.01
Chr2 mRNA 70920137 70923760 70920137 ID=MsG0280010522.01.T01;Parent=MsG0280010522.01;Name=MsG0280010522.01.T01;_AED=0.47;_eAED=0.47;_QI=384|0.75|0.8|1|1|1|5|501|370
Chr2 exon 70920137 70920873 70920137 ID=MsG0280010522.01.T01:exon:10056;Parent=MsG0280010522.01.T01
Chr2 exon 70921505 70921625 70921505 ID=MsG0280010522.01.T01:exon:10057;Parent=MsG0280010522.01.T01
Chr2 exon 70921732 70921851 70921732 ID=MsG0280010522.01.T01:exon:10058;Parent=MsG0280010522.01.T01
Chr2 exon 70922616 70922850 70922616 ID=MsG0280010522.01.T01:exon:10059;Parent=MsG0280010522.01.T01
Chr2 exon 70922976 70923760 70922976 ID=MsG0280010522.01.T01:exon:10060;Parent=MsG0280010522.01.T01
Chr2 five_prime_UTR 70920137 70920520 70920137 ID=MsG0280010522.01.T01:five_prime_utr;Parent=MsG0280010522.01.T01
Chr2 CDS 70920521 70920873 70920521 ID=MsG0280010522.01.T01:cds;Parent=MsG0280010522.01.T01
Chr2 CDS 70921505 70921625 70921505 ID=MsG0280010522.01.T01:cds;Parent=MsG0280010522.01.T01
Chr2 CDS 70921732 70921851 70921732 ID=MsG0280010522.01.T01:cds;Parent=MsG0280010522.01.T01
Chr2 CDS 70922616 70922850 70922616 ID=MsG0280010522.01.T01:cds;Parent=MsG0280010522.01.T01
Chr2 CDS 70922976 70923259 70922976 ID=MsG0280010522.01.T01:cds;Parent=MsG0280010522.01.T01
Chr2 three_prime_UTR 70923260 70923760 70923260 ID=MsG0280010522.01.T01:three_prime_utr;Parent=MsG0280010522.01.T01
Gene Sequence

>MsG0280010522.01.T01

ATGACAGAAGTTCTCCATTCTCCACCTCATTTTACTTCATCATCAACAACTACAACACCAACACCTTCAACTTCAACTTCAACTTCTTCTCCTCTCTCTTCATTCTCTTGCCATGCCATTTTTCAACCTCCTTCGCAACCACATTTTCCACTTTTTGAGCTACTAGTAACTCTTTTCAGAAAATCATTATTTCCTTTCAAGAGTAGTGGCAACAAAGACCTTTGCAACATGGACATAAGTCCTCCAACAAACGTGCGCCATGTCGCACACGTTACTTTTGATAGGTTCAATGGTTTCTTGGGTTTGCCTGATGAGTTTGAACCTGATTTTCCCAGAAGGCCTCCTAGTGCTAGTGCAACTGTTTTTGGAGTTTCAACAGAATCTATGCAGTTATCTTATGATTCAAGAGGGAACAGTGTGCCAACAATTCTTCTGTTAATGCAAAGGCATCTGTATGTTCAAGAAGGGTTGCAGGTGGAGGGGATTTTCAGAATTAATGCAGACAATAGTCAAGAGGAACATGTTAGGAATCAATTGAACTTAGGATTGGTTCCTGAAGACATTGATGTACATTGTCTGGCAGGACTAATTAAGGCTTGGTTTCGGGAACTTCCTAGCGGCGTTCTGGACTCATTATCACAAGAGCAAGTAATGCAATGCCAAACTGAGGAAGACTGTATTGAACTAGTGAGGCATCTACCTCATGCTGAAGCTGCACTCTTGGATTGGGCCATCAATCTAATGGCTGATGTAGTCGAACACGAAAATCTCAATAAGATGAACGCGCGCAACATTGCCATGGTTTTTGCGCCAAACATGACTCAGGTCTATGGCAGATCCATTCACTGCATTGATGTACGCAGTTCAAGTGATGAATTTCTTGAAAATGCTTATATAAGGACGCTACGAGAAAGAAAGGATTCTATCGTCGAATCTAATCCAAGATTGAATTTAGAACCTTCTGATGAGAATGTACACCGTAGACTTTTCGAGTCATTTCAGAAAGAAGATGCTGCAGCAGCAGACAATGAAGAGGCTAAAGAAATTTTTGTTACAGAAAAAAACTGTCTTAGAATGTACATCCGAATCACTCGAAAAAAACTCCTCAACTGA
Protein sequence

>MsG0280010522.01.T01

MTEVLHSPPHFTSSSTTTTPTPSTSTSTSSPLSSFSCHAIFQPPSQPHFPLFELLVTLFRKSLFPFKSSGNKDLCNMDISPPTNVRHVAHVTFDRFNGFLGLPDEFEPDFPRRPPSASATVFGVSTESMQLSYDSRGNSVPTILLLMQRHLYVQEGLQVEGIFRINADNSQEEHVRNQLNLGLVPEDIDVHCLAGLIKAWFRELPSGVLDSLSQEQVMQCQTEEDCIELVRHLPHAEAALLDWAINLMADVVEHENLNKMNARNIAMVFAPNMTQVYGRSIHCIDVRSSSDEFLENAYIRTLRERKDSIVESNPRLNLEPSDENVHRRLFESFQKEDAAAADNEEAKEIFVTEKNCLRMYIRITRKKLLN*