AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005758.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005758.01.T01 MTR_1g106095 87.500 32 4 0 63 94 66 97 5.26e-16 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGATTTCATGGAATTAAT+AGG 0.269473 1:+95755342 None:intergenic
ACTGACCAATCCTGTGAATT+AGG 0.284322 1:-95757022 None:intergenic
ACAAATGAAATTTCTTATAA+AGG 0.286353 1:-95757071 None:intergenic
TATCCGGCGCCGGAGACTTA+TGG 0.337883 1:+95755366 MsG0180005758.01.T01:CDS
CATGGAATTAATAGGTTATC+CGG 0.342137 1:+95755350 None:intergenic
AGACATTACTTTGCACAGAT+TGG 0.375088 1:+95756873 MsG0180005758.01.T01:CDS
CCACCTCGTCAGCAAAATTC+AGG 0.380629 1:+95755516 MsG0180005758.01.T01:CDS
ACAAGGTTACCCTCCACAAC+AGG 0.383576 1:+95755422 MsG0180005758.01.T01:CDS
TCTCTGCTGTTGCTGTCTCT+TGG 0.422721 1:+95756834 MsG0180005758.01.T01:CDS
TTTCGTACTGCCTAATTCAC+AGG 0.460541 1:+95757012 MsG0180005758.01.T01:three_prime_UTR
TTTCCATAAGTCTCCGGCGC+CGG 0.467483 1:-95755369 None:intergenic
TACTGCCTAATTCACAGGAT+TGG 0.486618 1:+95757017 MsG0180005758.01.T01:three_prime_UTR
TAACCTTGTGGAGGATATCC+TGG 0.496681 1:-95755408 None:intergenic
GAATTAGGCAGTACGAAAGT+TGG 0.503589 1:-95757007 None:intergenic
AAGGTAACAAGCATCGCAAA+AGG 0.524557 1:-95757052 None:intergenic
TGCGGTGGGTAGCCTTGTTG+TGG 0.552033 1:-95755468 None:intergenic
TGTGGAGGATATCCTGGTGG+AGG 0.557895 1:-95755402 None:intergenic
TGAGGTGGATAGCCCTGTTG+TGG 0.565061 1:-95755435 None:intergenic
ATTAATAGGTTATCCGGCGC+CGG 0.592144 1:+95755356 MsG0180005758.01.T01:CDS
GGTGGATAGCCCTGTTGTGG+AGG 0.592582 1:-95755432 None:intergenic
TACTGAGGAGCATATTGCGG+TGG 0.605867 1:-95755483 None:intergenic
GTGGAGGATATCCTGGTGGA+GGG 0.625281 1:-95755401 None:intergenic
CAAGGCTATCCTCCACAACA+AGG 0.626755 1:+95755456 MsG0180005758.01.T01:CDS
GGTGGGTAGCCTTGTTGTGG+AGG 0.627143 1:-95755465 None:intergenic
AAAGATGCTTACCCTCCACC+AGG 0.636903 1:+95755390 MsG0180005758.01.T01:CDS
CAACAGGGCTATCCACCTCA+AGG 0.638659 1:+95755438 MsG0180005758.01.T01:CDS
GGTGGTGGCTGAGCGTACTG+AGG 0.643562 1:-95755498 None:intergenic
ACTGAGGAGCATATTGCGGT+GGG 0.646934 1:-95755482 None:intergenic
TGTTGTGGAGGGTAACCTTG+TGG 0.649835 1:-95755420 None:intergenic
GTGGATAGCCCTGTTGTGGA+GGG 0.654497 1:-95755431 None:intergenic
CAAGGTTACCCTCCACAACA+GGG 0.654753 1:+95755423 MsG0180005758.01.T01:CDS
CCACCAGGATATCCTCCACA+AGG 0.654889 1:+95755405 MsG0180005758.01.T01:CDS
TGTTGTGGAGGATAGCCTTG+AGG 0.658505 1:-95755453 None:intergenic
TGTGGAGGATAGCCTTGAGG+TGG 0.659468 1:-95755450 None:intergenic
GCGTACTGAGGAGCATATTG+CGG 0.667195 1:-95755486 None:intergenic
CCTCGTCAGCAAAATTCAGG+CGG 0.710460 1:+95755519 MsG0180005758.01.T01:CDS
CCTTGTGGAGGATATCCTGG+TGG 0.714352 1:-95755405 None:intergenic
TGTGGAGGGTAACCTTGTGG+AGG 0.722140 1:-95755417 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGAAATTAAAATTGAA+TGG + Chr1:95755637-95755656 MsG0180005758.01.T01:intron 10.0%
!!! TTTAATTTCAATTTTAACTT+GGG - Chr1:95755631-95755650 None:intergenic 10.0%
!!! TTTTAATTTCAATTTTAACT+TGG - Chr1:95755632-95755651 None:intergenic 10.0%
!! AAAAGAATAAGAAACTAATA+AGG - Chr1:95756680-95756699 None:intergenic 15.0%
!! AACAAGCTATTAAAAATTAA+AGG + Chr1:95755917-95755936 MsG0180005758.01.T01:intron 15.0%
!! ACAAATGAAATTTCTTATAA+AGG - Chr1:95757074-95757093 None:intergenic 15.0%
!!! TTATTAGTTTCTTATTCTTT+TGG + Chr1:95756679-95756698 MsG0180005758.01.T01:intron 15.0%
!!! TTTTCTTTCTTTATTTTTGT+GGG + Chr1:95755817-95755836 MsG0180005758.01.T01:intron 15.0%
!!! TTTTTCTTTCTTTATTTTTG+TGG + Chr1:95755816-95755835 MsG0180005758.01.T01:intron 15.0%
!! AATTTGCAAACAGAAAATTA+AGG - Chr1:95755601-95755620 None:intergenic 20.0%
!! ACAATACTAGTATACTAAAA+AGG + Chr1:95756335-95756354 MsG0180005758.01.T01:intron 20.0%
!! TCAAAAACCTTATTAATTTC+TGG + Chr1:95756728-95756747 MsG0180005758.01.T01:intron 20.0%
!! TTGACAAACAGAAATTAATA+AGG - Chr1:95756712-95756731 None:intergenic 20.0%
!!! AATAGCTTGTTTGTTAATTT+AGG - Chr1:95755908-95755927 None:intergenic 20.0%
! AAAGAACAAAGATAAACGTA+TGG - Chr1:95756541-95756560 None:intergenic 25.0%
! AAATGAAATGAACGTGATTA+AGG + Chr1:95755666-95755685 MsG0180005758.01.T01:intron 25.0%
! CAGAAAACCAGAAATTAATA+AGG - Chr1:95756738-95756757 None:intergenic 25.0%
! CATTCAATTAATGATGTATG+TGG + Chr1:95756117-95756136 MsG0180005758.01.T01:intron 25.0%
! CTGCAACATTTACAAATTAT+AGG - Chr1:95756805-95756824 None:intergenic 25.0%
! TTTGAACTTAAAATATCCTC+CGG + Chr1:95756060-95756079 MsG0180005758.01.T01:intron 25.0%
!! TTGATATTTCTAAACTCTTG+TGG - Chr1:95756445-95756464 None:intergenic 25.0%
!! TTGCATTAATATGCATTTTG+AGG - Chr1:95756570-95756589 None:intergenic 25.0%
!! TTTTTGCTTATATTAGTGAC+CGG - Chr1:95756082-95756101 None:intergenic 25.0%
!!! AGTAGGTTGGTTTTAATTTT+AGG - Chr1:95755958-95755977 None:intergenic 25.0%
AATAGTATGTCCGATGATAT+GGG - Chr1:95755844-95755863 None:intergenic 30.0%
AATGCAAATGCAACAATTCT+TGG + Chr1:95756583-95756602 MsG0180005758.01.T01:intron 30.0%
AATTTGTAAATGTTGCAGCT+TGG + Chr1:95756807-95756826 MsG0180005758.01.T01:intron 30.0%
ATAACGTGACGAAAGATAAT+AGG - Chr1:95755776-95755795 None:intergenic 30.0%
CTAGTATACTAAAAAGGTGA+AGG + Chr1:95756341-95756360 MsG0180005758.01.T01:intron 30.0%
GTGTTTGTATAACTGGAAAT+AGG - Chr1:95756647-95756666 None:intergenic 30.0%
TAATAGTATGTCCGATGATA+TGG - Chr1:95755845-95755864 None:intergenic 30.0%
TATAGTTGTTGAGTACTAGT+AGG - Chr1:95755975-95755994 None:intergenic 30.0%
TATTTGCTTGTATTAGTGAC+CGG + Chr1:95756191-95756210 MsG0180005758.01.T01:intron 30.0%
TTTGTATAACTGGAAATAGG+TGG - Chr1:95756644-95756663 None:intergenic 30.0%
! CGTTTATCTTTGTTCTTTTG+TGG + Chr1:95756543-95756562 MsG0180005758.01.T01:intron 30.0%
! GACAATATACCATTTCTAGA+TGG - Chr1:95756290-95756309 None:intergenic 30.0%
! TGGTATATTGTCTTGTTCAA+CGG + Chr1:95756298-95756317 MsG0180005758.01.T01:intron 30.0%
AGACATTACTTTGCACAGAT+TGG + Chr1:95756873-95756892 MsG0180005758.01.T01:CDS 35.0%
AGTGTATAAGTCAAGTGGAA+GGG + Chr1:95756214-95756233 MsG0180005758.01.T01:intron 35.0%
CAGATCAGATTACATCACAT+TGG - Chr1:95756780-95756799 None:intergenic 35.0%
TAAGGAACAATCCAGAAAGA+TGG - Chr1:95755583-95755602 None:intergenic 35.0%
TACGTGTGTGTTTGTATAAC+TGG - Chr1:95756654-95756673 None:intergenic 35.0%
TGAAATGAACGTGATTAAGG+AGG + Chr1:95755669-95755688 MsG0180005758.01.T01:intron 35.0%
AATGATGTCAATGACGTGCA+GGG + Chr1:95756259-95756278 MsG0180005758.01.T01:intron 40.0%
ACTGACCAATCCTGTGAATT+AGG - Chr1:95757025-95757044 None:intergenic 40.0%
AGGGTTTAGCCATCTAGAAA+TGG + Chr1:95756278-95756297 MsG0180005758.01.T01:intron 40.0%
GAATTAGGCAGTACGAAAGT+TGG - Chr1:95757010-95757029 None:intergenic 40.0%
GAGTGTATAAGTCAAGTGGA+AGG + Chr1:95756213-95756232 MsG0180005758.01.T01:intron 40.0%
GGAGATTCATTCAGATCAAG+CGG + Chr1:95755690-95755709 MsG0180005758.01.T01:intron 40.0%
GTGTATAAGTCAAGTGGAAG+GGG + Chr1:95756215-95756234 MsG0180005758.01.T01:intron 40.0%
GTTGTTGAGTACTAGTAGGT+TGG - Chr1:95755971-95755990 None:intergenic 40.0%
TAATGATGTCAATGACGTGC+AGG + Chr1:95756258-95756277 MsG0180005758.01.T01:intron 40.0%
TACTGCCTAATTCACAGGAT+TGG + Chr1:95757017-95757036 MsG0180005758.01.T01:three_prime_UTR 40.0%
TCCACTTGACTTATACACTC+CGG - Chr1:95756213-95756232 None:intergenic 40.0%
TTGCTTATATTAGTGACCGG+AGG - Chr1:95756079-95756098 None:intergenic 40.0%
TTTCGTACTGCCTAATTCAC+AGG + Chr1:95757012-95757031 MsG0180005758.01.T01:three_prime_UTR 40.0%
! AAGGTAACAAGCATCGCAAA+AGG - Chr1:95757055-95757074 None:intergenic 40.0%
! GCATCTTTTCCATAAGTCTC+CGG - Chr1:95755378-95755397 None:intergenic 40.0%
! TAAAAAGGTGAAGGCGTATG+TGG + Chr1:95756350-95756369 MsG0180005758.01.T01:intron 40.0%
! TTTTGTGGGACCCATATCAT+CGG + Chr1:95755831-95755850 MsG0180005758.01.T01:intron 40.0%
ACCGGAGTGTATAAGTCAAG+TGG + Chr1:95756209-95756228 MsG0180005758.01.T01:intron 45.0%
ATAAGTCAAGTGGAAGGGGT+GGG + Chr1:95756219-95756238 MsG0180005758.01.T01:intron 45.0%
ATCTTGGAATTGATCGCTGG+TGG + Chr1:95755717-95755736 MsG0180005758.01.T01:intron 45.0%
ATTAATAGGTTATCCGGCGC+CGG + Chr1:95755356-95755375 MsG0180005758.01.T01:CDS 45.0%
CAATCCAGAAAGATGGAGAG+AGG - Chr1:95755576-95755595 None:intergenic 45.0%
TAACCTTGTGGAGGATATCC+TGG - Chr1:95755411-95755430 None:intergenic 45.0%
TACTCCTCTCTCCATCTTTC+TGG + Chr1:95755569-95755588 MsG0180005758.01.T01:intron 45.0%
TATAAGTCAAGTGGAAGGGG+TGG + Chr1:95756218-95756237 MsG0180005758.01.T01:intron 45.0%
!! TCTAATAATAAAATTAAAAA+AGG - Chr1:95756397-95756416 None:intergenic 5.0%
AAAGATGCTTACCCTCCACC+AGG + Chr1:95755390-95755409 MsG0180005758.01.T01:CDS 50.0%
ACAAGGTTACCCTCCACAAC+AGG + Chr1:95755422-95755441 MsG0180005758.01.T01:CDS 50.0%
CAAGGCTATCCTCCACAACA+AGG + Chr1:95755456-95755475 MsG0180005758.01.T01:CDS 50.0%
CAAGGTTACCCTCCACAACA+GGG + Chr1:95755423-95755442 MsG0180005758.01.T01:CDS 50.0%
CCACCTCGTCAGCAAAATTC+AGG + Chr1:95755516-95755535 MsG0180005758.01.T01:CDS 50.0%
CCTCGTCAGCAAAATTCAGG+CGG + Chr1:95755519-95755538 MsG0180005758.01.T01:CDS 50.0%
GCGATCTTGGAATTGATCGC+TGG + Chr1:95755714-95755733 MsG0180005758.01.T01:intron 50.0%
TCTCTGCTGTTGCTGTCTCT+TGG + Chr1:95756834-95756853 MsG0180005758.01.T01:CDS 50.0%
TGTTGTGGAGGGTAACCTTG+TGG - Chr1:95755423-95755442 None:intergenic 50.0%
! ACTGAGGAGCATATTGCGGT+GGG - Chr1:95755485-95755504 None:intergenic 50.0%
! CCTGAATTTTGCTGACGAGG+TGG - Chr1:95755519-95755538 None:intergenic 50.0%
! CTCTTGGATGCGTGCTTTTG+AGG + Chr1:95756850-95756869 MsG0180005758.01.T01:CDS 50.0%
! GAATTTTGCTGACGAGGTGG+TGG - Chr1:95755516-95755535 None:intergenic 50.0%
! GCGTACTGAGGAGCATATTG+CGG - Chr1:95755489-95755508 None:intergenic 50.0%
! TACTGAGGAGCATATTGCGG+TGG - Chr1:95755486-95755505 None:intergenic 50.0%
! TGTTGTGGAGGATAGCCTTG+AGG - Chr1:95755456-95755475 None:intergenic 50.0%
CAACAGGGCTATCCACCTCA+AGG + Chr1:95755438-95755457 MsG0180005758.01.T01:CDS 55.0%
CAGATCAAGCGGTGCGATCT+TGG + Chr1:95755701-95755720 MsG0180005758.01.T01:intron 55.0%
CAGCAAAATTCAGGCGGTGC+TGG + Chr1:95755525-95755544 MsG0180005758.01.T01:intron 55.0%
CCACCAGGATATCCTCCACA+AGG + Chr1:95755405-95755424 MsG0180005758.01.T01:CDS 55.0%
CCTTGTGGAGGATATCCTGG+TGG - Chr1:95755408-95755427 None:intergenic 55.0%
GTGGAGGATATCCTGGTGGA+GGG - Chr1:95755404-95755423 None:intergenic 55.0%
GTGGATAGCCCTGTTGTGGA+GGG - Chr1:95755434-95755453 None:intergenic 55.0%
TGAGGTGGATAGCCCTGTTG+TGG - Chr1:95755438-95755457 None:intergenic 55.0%
TGTGGAGGATATCCTGGTGG+AGG - Chr1:95755405-95755424 None:intergenic 55.0%
TGTGGAGGGTAACCTTGTGG+AGG - Chr1:95755420-95755439 None:intergenic 55.0%
TTTCCATAAGTCTCCGGCGC+CGG - Chr1:95755372-95755391 None:intergenic 55.0%
! CCGCCTGAATTTTGCTGACG+AGG - Chr1:95755522-95755541 None:intergenic 55.0%
! TGTGGAGGATAGCCTTGAGG+TGG - Chr1:95755453-95755472 None:intergenic 55.0%
GGTGGATAGCCCTGTTGTGG+AGG - Chr1:95755435-95755454 None:intergenic 60.0%
! TATCCGGCGCCGGAGACTTA+TGG + Chr1:95755366-95755385 MsG0180005758.01.T01:CDS 60.0%
!! GGTGGGTAGCCTTGTTGTGG+AGG - Chr1:95755468-95755487 None:intergenic 60.0%
!! GTGCTGGTTGCATGGAAGGC+TGG + Chr1:95755541-95755560 MsG0180005758.01.T01:intron 60.0%
!! TGCGGTGGGTAGCCTTGTTG+TGG - Chr1:95755471-95755490 None:intergenic 60.0%
!! TTCAGGCGGTGCTGGTTGCA+TGG + Chr1:95755533-95755552 MsG0180005758.01.T01:intron 60.0%
! GGTGGTGGCTGAGCGTACTG+AGG - Chr1:95755501-95755520 None:intergenic 65.0%
!! GGCGGTGCTGGTTGCATGGA+AGG + Chr1:95755537-95755556 MsG0180005758.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 95755351 95757123 95755351 ID=MsG0180005758.01;Name=MsG0180005758.01
Chr1 mRNA 95755351 95757123 95755351 ID=MsG0180005758.01.T01;Parent=MsG0180005758.01;Name=MsG0180005758.01.T01;_AED=0.41;_eAED=0.42;_QI=0|0|0.5|1|0|0|2|192|98
Chr1 exon 95755351 95755540 95755351 ID=MsG0180005758.01.T01:exon:19394;Parent=MsG0180005758.01.T01
Chr1 exon 95756825 95757123 95756825 ID=MsG0180005758.01.T01:exon:19395;Parent=MsG0180005758.01.T01
Chr1 CDS 95755351 95755540 95755351 ID=MsG0180005758.01.T01:cds;Parent=MsG0180005758.01.T01
Chr1 CDS 95756825 95756931 95756825 ID=MsG0180005758.01.T01:cds;Parent=MsG0180005758.01.T01
Chr1 three_prime_UTR 95756932 95757123 95756932 ID=MsG0180005758.01.T01:three_prime_utr;Parent=MsG0180005758.01.T01
Gene Sequence

>MsG0180005758.01.T01

ATGGAATTAATAGGTTATCCGGCGCCGGAGACTTATGGAAAAGATGCTTACCCTCCACCAGGATATCCTCCACAAGGTTACCCTCCACAACAGGGCTATCCACCTCAAGGCTATCCTCCACAACAAGGCTACCCACCGCAATATGCTCCTCAGTACGCTCAGCCACCACCTCGTCAGCAAAATTCAGGCGCTTGGCTGCTCTCTGCTGTTGCTGTCTCTTGGATGCGTGCTTTTGAGGAGACATTACTTTGCACAGATTGGATTGATTGCATGTGTATTTTGAATCTGAGCACATAA

Protein sequence

>MsG0180005758.01.T01

MELIGYPAPETYGKDAYPPPGYPPQGYPPQQGYPPQGYPPQQGYPPQYAPQYAQPPPRQQNSGAWLLSAVAVSWMRAFEETLLCTDWIDCMCILNLST*