AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011194.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011194.01.T01 MTR_2g099940 70.330 364 56 4 28 353 18 367 6.76e-131 379
MsG0280011194.01.T01 MTR_2g039680 56.721 305 78 6 24 290 16 304 1.68e-90 275
MsG0280011194.01.T01 MTR_2g039680 56.721 305 78 6 24 290 16 304 4.94e-90 275
MsG0280011194.01.T01 MTR_2g039697 48.929 280 111 6 30 283 23 296 2.75e-74 233
MsG0280011194.01.T01 MTR_2g039700 44.099 322 103 9 13 290 12 300 1.27e-60 198
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011194.01.T01 AT2G19480 52.547 373 120 6 23 353 15 372 1.86e-89 273
MsG0280011194.01.T01 AT2G19480 55.000 300 81 5 23 283 15 299 3.42e-88 270
MsG0280011194.01.T01 AT4G26110 53.642 302 87 4 23 285 15 302 3.54e-88 270
MsG0280011194.01.T01 AT2G19480 55.000 300 81 5 23 283 15 299 4.11e-88 270
MsG0280011194.01.T01 AT4G26110 53.642 302 87 4 23 285 15 302 5.54e-88 269
MsG0280011194.01.T01 AT5G56950 51.743 373 123 7 24 353 16 374 2.85e-87 268
MsG0280011194.01.T01 AT3G13782 45.946 259 98 7 55 283 52 298 1.95e-53 179
MsG0280011194.01.T01 AT3G13782 45.946 259 98 7 55 283 52 298 1.95e-53 179

Find 78 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAGCATGATTATGATAT+TGG 0.178236 2:-80814894 MsG0280011194.01.T01:CDS
CGTCATCATCCTCGGGAATC+TGG 0.256403 2:+80815039 None:intergenic
TGAAGAGCTTCAAAATTTAA+TGG 0.286494 2:-80814913 MsG0280011194.01.T01:CDS
CCAATAGATAAGCTAAAATA+AGG 0.306745 2:+80816105 None:intergenic
TGACTGATCTTTACCAGGTT+TGG 0.330775 2:+80813880 None:intergenic
CTTTACACAAAGGTGGTGTT+TGG 0.363650 2:-80816132 MsG0280011194.01.T01:CDS
GTAGGATAAGCTTCAGCTCT+TGG 0.371162 2:-80816793 MsG0280011194.01.T01:intron
AAAGTGGTTCATAAAGCTTC+TGG 0.375481 2:+80816151 None:intergenic
ATCAATTTCGTCATCATCCT+CGG 0.387289 2:+80815031 None:intergenic
GATAAGCTTCAGCTCTTGGC+TGG 0.400903 2:-80816789 MsG0280011194.01.T01:CDS
TGGAGCATGATTATGATATT+GGG 0.401577 2:-80814893 MsG0280011194.01.T01:intron
GTCATCATCCTCGGGAATCT+GGG 0.405862 2:+80815040 None:intergenic
TCATGCTGTGTCATGGTTCA+CGG 0.413395 2:-80814226 MsG0280011194.01.T01:CDS
AGGAGGAAGACGAAGGGAAG+GGG 0.415086 2:-80814086 MsG0280011194.01.T01:CDS
GATCTTCCCGCTGCCGCCGC+CGG 0.433634 2:-80817209 MsG0280011194.01.T01:intron
GCAGAGCGAACATGATGAAT+TGG 0.440473 2:-80816217 MsG0280011194.01.T01:intron
TCAACACGTTGCCTGACATT+AGG 0.442995 2:+80816735 None:intergenic
TACTGCTGCTTGCAATCAGC+AGG 0.443739 2:+80813848 None:intergenic
AACTCCAGCTTGAAACCCTT+GGG 0.448772 2:+80815377 None:intergenic
GAGGAGGAAGACGAAGGGAA+GGG 0.455702 2:-80814087 MsG0280011194.01.T01:CDS
AACACCACCTTTGTGTAAAG+TGG 0.458063 2:+80816135 None:intergenic
ATGTTACCGGCGGCGGCAGC+GGG 0.460017 2:+80817203 None:intergenic
TTTGTGTCAAGCACTTTCCT+GGG 0.461063 2:+80815159 None:intergenic
TGAGGAGGAAGACGAAGGGA+AGG 0.464687 2:-80814088 MsG0280011194.01.T01:CDS
AATTACAAACGAAGCAGGAC+AGG 0.467567 2:-80815722 MsG0280011194.01.T01:CDS
GATGTTACCGGCGGCGGCAG+CGG 0.475914 2:+80817202 None:intergenic
AAACTCCAGCTTGAAACCCT+TGG 0.483887 2:+80815376 None:intergenic
TTTAGAGGAGCGAGCTCAGT+TGG 0.495888 2:-80816184 MsG0280011194.01.T01:CDS
GGCGGCGGCAGCGGGAAGAT+CGG 0.499586 2:+80817211 None:intergenic
CATGCTGTGTCATGGTTCAC+GGG 0.510537 2:-80814225 MsG0280011194.01.T01:CDS
AGACAGAGATGAAGATGAAG+AGG 0.511035 2:-80814151 MsG0280011194.01.T01:CDS
CAATCCCAAGGGTTTCAAGC+TGG 0.511184 2:-80815381 MsG0280011194.01.T01:CDS
ATAAGCTTCAGCTCTTGGCT+GGG 0.519230 2:-80816788 MsG0280011194.01.T01:CDS
AAACACTTTCGCCTAATGTC+AGG 0.521826 2:-80816746 MsG0280011194.01.T01:CDS
AGGAAAGTGCTTGACACAAA+AGG 0.521935 2:-80815156 MsG0280011194.01.T01:CDS
TGAGGATGAAGATGATGAGG+AGG 0.524386 2:-80814103 MsG0280011194.01.T01:CDS
ATGATGAGGAGGAAGACGAA+GGG 0.532918 2:-80814092 MsG0280011194.01.T01:CDS
GATGATGAGGAGGAAGACGA+AGG 0.535181 2:-80814093 MsG0280011194.01.T01:CDS
GGGAAGATCGGCGGATTGAT+CGG 0.536009 2:+80817223 None:intergenic
TCAATTTCGTCATCATCCTC+GGG 0.537035 2:+80815032 None:intergenic
TCATCCTCGGGAATCTGGGG+AGG 0.537400 2:+80815044 None:intergenic
TGATGACGATGAAGAGGATG+AGG 0.541376 2:-80814121 MsG0280011194.01.T01:CDS
TAATGTCAGGCAACGTGTTG+AGG 0.542732 2:-80816733 MsG0280011194.01.T01:CDS
GGATGAGGATGAAGATGATG+AGG 0.546840 2:-80814106 MsG0280011194.01.T01:CDS
TCAGTTGACTGATCTTTACC+AGG 0.547286 2:+80813875 None:intergenic
AGTGGTAGCAAGGCCAAACC+TGG 0.552380 2:-80813893 MsG0280011194.01.T01:CDS
TAAGCTTCAGCTCTTGGCTG+GGG 0.556329 2:-80816787 MsG0280011194.01.T01:CDS
TTATTCCTCATGCTGTGTCA+TGG 0.556398 2:-80814233 MsG0280011194.01.T01:CDS
TGTGTCATGGTTCACGGGAG+AGG 0.556613 2:-80814220 MsG0280011194.01.T01:CDS
AGTATCTCAAAGATATCAAG+TGG 0.556691 2:-80815415 MsG0280011194.01.T01:CDS
GGCGGCAGCGGGAAGATCGG+CGG 0.558135 2:+80817214 None:intergenic
TAGAGGTACGAGATTGTCAA+TGG 0.558349 2:-80815763 MsG0280011194.01.T01:CDS
CACAAAAGGTTATGAAGAAG+AGG 0.558627 2:-80815142 MsG0280011194.01.T01:CDS
ATGAAGAAGAGGCCAAAGAA+AGG 0.565048 2:-80815131 MsG0280011194.01.T01:CDS
AATGGTGTTATTGACGTTGA+AGG 0.565526 2:-80815745 MsG0280011194.01.T01:CDS
GAAATTGATGTTACCGGCGG+CGG 0.568217 2:+80817196 None:intergenic
TGGGGATCGTGTGGATGTCA+TGG 0.574954 2:-80816769 MsG0280011194.01.T01:CDS
CATCCTCGGGAATCTGGGGA+GGG 0.576916 2:+80815045 None:intergenic
CTCAGTGCTGAAGATCGTGC+TGG 0.579116 2:-80817025 MsG0280011194.01.T01:CDS
ACTGCTGCTTGCAATCAGCA+GGG 0.579841 2:+80813849 None:intergenic
ACGGAAATTGAGTGGCACCC+AGG 0.582717 2:-80815176 MsG0280011194.01.T01:CDS
GCTCTTGGCTGGGGATCGTG+TGG 0.587567 2:-80816778 MsG0280011194.01.T01:CDS
GAAGGAATTACAAACGAAGC+AGG 0.588859 2:-80815727 MsG0280011194.01.T01:CDS
TGATGATGATGACGATGAAG+AGG 0.595217 2:-80814127 MsG0280011194.01.T01:CDS
TTAACTGCAGAGTGGTAGCA+AGG 0.597517 2:-80813903 MsG0280011194.01.T01:intron
TGAACCACTTTACACAAAGG+TGG 0.605805 2:-80816139 MsG0280011194.01.T01:CDS
TCATCATCCTCGGGAATCTG+GGG 0.622634 2:+80815041 None:intergenic
GAAGGGGAAGAACAAGAGCA+AGG 0.624049 2:-80814070 MsG0280011194.01.T01:intron
GAAGAAAGCAAAGCTGCTGA+AGG 0.627950 2:-80815697 MsG0280011194.01.T01:intron
CAACCCTCCCCAGATTCCCG+AGG 0.628448 2:-80815048 MsG0280011194.01.T01:CDS
GAGTAAACTTGACAATCCCA+AGG 0.632238 2:-80815393 MsG0280011194.01.T01:CDS
GATCACTGAACGTGATGAGG+AGG 0.639093 2:-80815444 MsG0280011194.01.T01:CDS
TTATGAACCACTTTACACAA+AGG 0.641292 2:-80816142 MsG0280011194.01.T01:CDS
ATTGTAGAACGGAAATTGAG+TGG 0.652316 2:-80815184 MsG0280011194.01.T01:intron
ATTACAAACGAAGCAGGACA+GGG 0.657529 2:-80815721 MsG0280011194.01.T01:CDS
ATAGATCACTGAACGTGATG+AGG 0.688272 2:-80815447 MsG0280011194.01.T01:intron
AGTAAACTTGACAATCCCAA+GGG 0.689310 2:-80815392 MsG0280011194.01.T01:CDS
GTGAACCATGACACAGCATG+AGG 0.722020 2:+80814228 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAATTGGAAA+AGG - Chr2:80816650-80816669 MsG0280011194.01.T01:intron 10.0%
!! AAGAATTAATATTAACTAAA+GGG - Chr2:80816620-80816639 MsG0280011194.01.T01:intron 10.0%
!!! TTATTTTCTCAAATTATTAT+GGG - Chr2:80814660-80814679 MsG0280011194.01.T01:intron 10.0%
!!! TTTATTTTCTCAAATTATTA+TGG - Chr2:80814659-80814678 MsG0280011194.01.T01:intron 10.0%
!! GAAGAATTAATATTAACTAA+AGG - Chr2:80816619-80816638 MsG0280011194.01.T01:intron 15.0%
!! GAAGAATTAATATTAACTTA+AGG - Chr2:80816677-80816696 MsG0280011194.01.T01:intron 15.0%
!! TATATGAAATAATTAACAGA+TGG + Chr2:80815585-80815604 None:intergenic 15.0%
!! TATTAACTTAAGGAAAAAAA+TGG - Chr2:80816687-80816706 MsG0280011194.01.T01:intron 15.0%
!!! CAATATCTTTTAATTTCTTA+GGG - Chr2:80815820-80815839 MsG0280011194.01.T01:intron 15.0%
!!! GCTATTTTTATAAGTTATAT+TGG + Chr2:80815082-80815101 None:intergenic 15.0%
!! AATGAAGACTAATGAAATAT+TGG - Chr2:80815517-80815536 MsG0280011194.01.T01:intron 20.0%
!! ATGAAGACTAATGAAATATT+GGG - Chr2:80815518-80815537 MsG0280011194.01.T01:intron 20.0%
!! CTATATTAGAGAAATCAATA+GGG - Chr2:80815793-80815812 MsG0280011194.01.T01:intron 20.0%
!! GAACTGATATTAACTTAAAA+CGG - Chr2:80816549-80816568 MsG0280011194.01.T01:intron 20.0%
!! GAAGAAGTAATATTAACTTA+AGG - Chr2:80816576-80816595 MsG0280011194.01.T01:intron 20.0%
!! TATTAACTTAAGGAAAAAGT+TGG - Chr2:80816586-80816605 MsG0280011194.01.T01:intron 20.0%
!! TCAAAAAACAAAATAGTACT+AGG + Chr2:80816778-80816797 None:intergenic 20.0%
!! TGTGATTAAATTATTTGTCA+CGG - Chr2:80814455-80814474 MsG0280011194.01.T01:intron 20.0%
!!! CATTTTTCTGTTTTTATGAT+TGG + Chr2:80815985-80816004 None:intergenic 20.0%
!!! CTATTGATTTCTCTAATATA+GGG + Chr2:80815794-80815813 None:intergenic 20.0%
!!! GAATTTACTCTGTTTTTATT+AGG - Chr2:80816313-80816332 MsG0280011194.01.T01:intron 20.0%
!!! GCAATATCTTTTAATTTCTT+AGG - Chr2:80815819-80815838 MsG0280011194.01.T01:intron 20.0%
!!! GTTTAATTTCTTTTTTGTTG+TGG - Chr2:80814746-80814765 MsG0280011194.01.T01:intron 20.0%
!!! TAGTACTATTTTGTTTTTTG+AGG - Chr2:80816777-80816796 MsG0280011194.01.T01:CDS 20.0%
!!! TATCTAAGTAATCAGAATTT+TGG - Chr2:80814121-80814140 MsG0280011194.01.T01:CDS 20.0%
!!! TATTATCTGTTGTTTGTTTA+TGG - Chr2:80815285-80815304 MsG0280011194.01.T01:intron 20.0%
! AACTTAAGGAAAAAAATGGT+GGG - Chr2:80816691-80816710 MsG0280011194.01.T01:intron 25.0%
! CCAATAGATAAGCTAAAATA+AGG + Chr2:80814972-80814991 None:intergenic 25.0%
! CCTATATTAGAGAAATCAAT+AGG - Chr2:80815792-80815811 MsG0280011194.01.T01:intron 25.0%
! TAACTTAAGGAAAAAAATGG+TGG - Chr2:80816690-80816709 MsG0280011194.01.T01:intron 25.0%
! TATCCAATTGATTAAAACCT+AGG - Chr2:80813995-80814014 MsG0280011194.01.T01:intron 25.0%
! TGAAGAGCTTCAAAATTTAA+TGG - Chr2:80816161-80816180 MsG0280011194.01.T01:CDS 25.0%
! TTGTTTGTTTATGGTTATAG+AGG - Chr2:80815294-80815313 MsG0280011194.01.T01:intron 25.0%
!! AAACTATAGTGTTGTTATGA+TGG - Chr2:80814179-80814198 MsG0280011194.01.T01:CDS 25.0%
!! AGTTTCATTTTACATTAGCA+AGG - Chr2:80814800-80814819 MsG0280011194.01.T01:intron 25.0%
!! GGATAATGATTTTGAATTAG+AGG + Chr2:80813980-80813999 None:intergenic 25.0%
!! GTTTCATTTTACATTAGCAA+GGG - Chr2:80814801-80814820 MsG0280011194.01.T01:intron 25.0%
!! TTTCAACTAATACTTTTGAG+TGG + Chr2:80816411-80816430 None:intergenic 25.0%
!! TTTTTATTAGGATTAGGAAG+AGG - Chr2:80816325-80816344 MsG0280011194.01.T01:intron 25.0%
!!! ACTCAAAAGTATTAGTTGAA+AGG - Chr2:80816410-80816429 MsG0280011194.01.T01:intron 25.0%
!!! ACTCTGTTTTTATTAGGATT+AGG - Chr2:80816319-80816338 MsG0280011194.01.T01:intron 25.0%
!!! AGTAGTTGATAAAGTTACTA+GGG + Chr2:80813951-80813970 None:intergenic 25.0%
!!! CCTATTGATTTCTCTAATAT+AGG + Chr2:80815795-80815814 None:intergenic 25.0%
!!! CCTTATTTTAGCTTATCTAT+TGG - Chr2:80814969-80814988 MsG0280011194.01.T01:intron 25.0%
!!! GTTTACATAAGCTATTTTCA+TGG + Chr2:80815148-80815167 None:intergenic 25.0%
!!! TAGTAGTTGATAAAGTTACT+AGG + Chr2:80813952-80813971 None:intergenic 25.0%
!!! TTCTGTTTTTATGATTGGTT+TGG + Chr2:80815980-80815999 None:intergenic 25.0%
AAGATTCAGGATAGTATCAA+AGG + Chr2:80815454-80815473 None:intergenic 30.0%
ACTTAAGGAAAAAAATGGTG+GGG - Chr2:80816692-80816711 MsG0280011194.01.T01:intron 30.0%
AGTATCTCAAAGATATCAAG+TGG - Chr2:80815659-80815678 MsG0280011194.01.T01:intron 30.0%
ATGGAGCATGATTATGATAT+TGG - Chr2:80816180-80816199 MsG0280011194.01.T01:CDS 30.0%
CTGAATTATACGAAAGATTC+AGG + Chr2:80815467-80815486 None:intergenic 30.0%
CTTAAGGAAAAAGTTGGAAA+AGG - Chr2:80816592-80816611 MsG0280011194.01.T01:intron 30.0%
CTTTCGTATAATTCAGAGAA+AGG - Chr2:80815471-80815490 MsG0280011194.01.T01:intron 30.0%
GAAAGTGAAATTGATGTTAC+CGG + Chr2:80813887-80813906 None:intergenic 30.0%
GGATACCAATATCATAATCA+TGG + Chr2:80816265-80816284 None:intergenic 30.0%
GTATCAAAGGAGAATGTATA+TGG + Chr2:80815441-80815460 None:intergenic 30.0%
TAATGAAATATTGGGTGAAG+AGG - Chr2:80815526-80815545 MsG0280011194.01.T01:intron 30.0%
TATAAGTTGTTTGTCCAAAC+AGG - Chr2:80815204-80815223 MsG0280011194.01.T01:intron 30.0%
TGAAGCCATGATTATGATAT+TGG - Chr2:80816257-80816276 MsG0280011194.01.T01:intron 30.0%
TGGAGCATGATTATGATATT+GGG - Chr2:80816181-80816200 MsG0280011194.01.T01:CDS 30.0%
TGTCACGGATACAAATATTT+GGG - Chr2:80814470-80814489 MsG0280011194.01.T01:intron 30.0%
TTAACTCTCTGCTAAACTAT+GGG - Chr2:80817112-80817131 MsG0280011194.01.T01:intron 30.0%
TTATGAACCACTTTACACAA+AGG - Chr2:80814932-80814951 MsG0280011194.01.T01:intron 30.0%
TTCACAAACAATTCCACAAA+GGG + Chr2:80814246-80814265 None:intergenic 30.0%
TTGAACGTAATCACATGTAT+CGG + Chr2:80814635-80814654 None:intergenic 30.0%
TTGTCACGGATACAAATATT+TGG - Chr2:80814469-80814488 MsG0280011194.01.T01:intron 30.0%
TTTCACAAACAATTCCACAA+AGG + Chr2:80814247-80814266 None:intergenic 30.0%
TTTGCTTGAACATTGTAGAA+CGG - Chr2:80815879-80815898 MsG0280011194.01.T01:intron 30.0%
! CATGAGGAATAATTTTGTCT+CGG + Chr2:80816833-80816852 None:intergenic 30.0%
! TACAAATATTTGGGATTGCT+TGG - Chr2:80814479-80814498 MsG0280011194.01.T01:intron 30.0%
! TCTTATTCTTTTGATGTGAG+TGG - Chr2:80816234-80816253 MsG0280011194.01.T01:intron 30.0%
!! AGACCTAGGTTTTAATCAAT+TGG + Chr2:80814001-80814020 None:intergenic 30.0%
!! ATTGGAGAGTAAGTTTTTAG+AGG - Chr2:80814875-80814894 MsG0280011194.01.T01:intron 30.0%
!! GTGATATGTTTTTGTTAGCA+CGG + Chr2:80815754-80815773 None:intergenic 30.0%
!!! ATTTTTTACTGTTCCCTTTG+TGG - Chr2:80814230-80814249 MsG0280011194.01.T01:CDS 30.0%
!!! GCATTTTTTGATCCTTTCTT+TGG + Chr2:80815958-80815977 None:intergenic 30.0%
AAAGTGGTTCATAAAGCTTC+TGG + Chr2:80814926-80814945 None:intergenic 35.0%
AATTGTAACCGTAGTGAAAC+AGG + Chr2:80815262-80815281 None:intergenic 35.0%
AGTAAACTTGACAATCCCAA+GGG - Chr2:80815682-80815701 MsG0280011194.01.T01:intron 35.0%
AGTTACTTCCTGTTTCACTA+CGG - Chr2:80815251-80815270 MsG0280011194.01.T01:intron 35.0%
ATCAATTTCGTCATCATCCT+CGG + Chr2:80816046-80816065 None:intergenic 35.0%
ATCATCCTCCTTCTATCTAT+TGG - Chr2:80814386-80814405 MsG0280011194.01.T01:intron 35.0%
ATCTATCACAGAAAATGGCA+TGG + Chr2:80815613-80815632 None:intergenic 35.0%
ATGAGACACCAATAGATAGA+AGG + Chr2:80814397-80814416 None:intergenic 35.0%
ATTGCAGTTAACCAAAGCTA+GGG + Chr2:80815500-80815519 None:intergenic 35.0%
ATTGTAGAACGGAAATTGAG+TGG - Chr2:80815890-80815909 MsG0280011194.01.T01:intron 35.0%
CACAAAAGGTTATGAAGAAG+AGG - Chr2:80815932-80815951 MsG0280011194.01.T01:intron 35.0%
CAGAATCCACAATCAGTAAT+CGG + Chr2:80817078-80817097 None:intergenic 35.0%
CAGTGATCTATCACAGAAAA+TGG + Chr2:80815618-80815637 None:intergenic 35.0%
CATCAGAAATTAGACACTAC+GGG + Chr2:80815230-80815249 None:intergenic 35.0%
CCATCAGAAATTAGACACTA+CGG + Chr2:80815231-80815250 None:intergenic 35.0%
CTTAACTCTCTGCTAAACTA+TGG - Chr2:80817111-80817130 MsG0280011194.01.T01:intron 35.0%
CTTAAGGAAAAAAATGGTGG+GGG - Chr2:80816693-80816712 MsG0280011194.01.T01:intron 35.0%
GCTACTCATTATAGAACACA+AGG + Chr2:80814562-80814581 None:intergenic 35.0%
GGGATACAGATTCCTTAATT+CGG - Chr2:80817029-80817048 MsG0280011194.01.T01:CDS 35.0%
GTGATGACAAGAATGAGAAA+TGG + Chr2:80813911-80813930 None:intergenic 35.0%
! ATACATTTTGCGACACTGTT+TGG - Chr2:80814993-80815012 MsG0280011194.01.T01:intron 35.0%
! GTTTTTGTTAGCACGGATTT+TGG + Chr2:80815747-80815766 None:intergenic 35.0%
! TACATTTTGCGACACTGTTT+GGG - Chr2:80814994-80815013 MsG0280011194.01.T01:intron 35.0%
!! AATGGTGTTATTGACGTTGA+AGG - Chr2:80815329-80815348 MsG0280011194.01.T01:intron 35.0%
!! AGCAACAGTATTTTAGACCT+AGG + Chr2:80814015-80814034 None:intergenic 35.0%
!! GAAATGTTTGCTGTTTCTGT+AGG - Chr2:80814263-80814282 MsG0280011194.01.T01:intron 35.0%
!! TACTCAATGAAGCATTGACA+AGG + Chr2:80814718-80814737 None:intergenic 35.0%
!! TAGCACGGATTTTGGAAATA+AGG + Chr2:80815739-80815758 None:intergenic 35.0%
!! TTAGTCTTGTTCAACAGTGT+AGG - Chr2:80815409-80815428 MsG0280011194.01.T01:CDS 35.0%
AAACACTTTCGCCTAATGTC+AGG - Chr2:80814328-80814347 MsG0280011194.01.T01:intron 40.0%
AACACCACCTTTGTGTAAAG+TGG + Chr2:80814942-80814961 None:intergenic 40.0%
AATTACAAACGAAGCAGGAC+AGG - Chr2:80815352-80815371 MsG0280011194.01.T01:CDS 40.0%
AGACACCAATAGATAGAAGG+AGG + Chr2:80814394-80814413 None:intergenic 40.0%
AGACAGAGATGAAGATGAAG+AGG - Chr2:80816923-80816942 MsG0280011194.01.T01:intron 40.0%
AGCATGAATTGTCTTCCTTC+CGG + Chr2:80816750-80816769 None:intergenic 40.0%
AGTGAAATTGATGTTACCGG+CGG + Chr2:80813884-80813903 None:intergenic 40.0%
ATAGATCACTGAACGTGATG+AGG - Chr2:80815627-80815646 MsG0280011194.01.T01:intron 40.0%
ATGAAGAAGAGGCCAAAGAA+AGG - Chr2:80815943-80815962 MsG0280011194.01.T01:intron 40.0%
ATTACAAACGAAGCAGGACA+GGG - Chr2:80815353-80815372 MsG0280011194.01.T01:CDS 40.0%
CAGCAGATTATGCCGAATTA+AGG + Chr2:80817044-80817063 None:intergenic 40.0%
CATTGCAGTTAACCAAAGCT+AGG + Chr2:80815501-80815520 None:intergenic 40.0%
CCGTAGTGTCTAATTTCTGA+TGG - Chr2:80815228-80815247 MsG0280011194.01.T01:intron 40.0%
CTTTACACAAAGGTGGTGTT+TGG - Chr2:80814942-80814961 MsG0280011194.01.T01:intron 40.0%
GAAGGAATTACAAACGAAGC+AGG - Chr2:80815347-80815366 MsG0280011194.01.T01:CDS 40.0%
GAGTAAACTTGACAATCCCA+AGG - Chr2:80815681-80815700 MsG0280011194.01.T01:intron 40.0%
GGACAAACAACTTATAGCAC+AGG + Chr2:80815200-80815219 None:intergenic 40.0%
GTTGAGTGCAAAACTGCTTA+TGG - Chr2:80816446-80816465 MsG0280011194.01.T01:intron 40.0%
TCAATTTCGTCATCATCCTC+GGG + Chr2:80816045-80816064 None:intergenic 40.0%
TCAGTTGACTGATCTTTACC+AGG + Chr2:80817202-80817221 None:intergenic 40.0%
TCTGCTAAACTATGGGTAAC+TGG - Chr2:80817119-80817138 MsG0280011194.01.T01:intron 40.0%
TGAACCACTTTACACAAAGG+TGG - Chr2:80814935-80814954 MsG0280011194.01.T01:intron 40.0%
TGACTGATCTTTACCAGGTT+TGG + Chr2:80817197-80817216 None:intergenic 40.0%
TGTTTGTCTTAACTGCAGAG+TGG - Chr2:80817163-80817182 MsG0280011194.01.T01:intron 40.0%
TTATTCCTCATGCTGTGTCA+TGG - Chr2:80816841-80816860 MsG0280011194.01.T01:intron 40.0%
TTTGTGTCAAGCACTTTCCT+GGG + Chr2:80815918-80815937 None:intergenic 40.0%
! ACACCTTCGATTAGAAGTGT+CGG + Chr2:80814597-80814616 None:intergenic 40.0%
! TAGAGGTACGAGATTGTCAA+TGG - Chr2:80815311-80815330 MsG0280011194.01.T01:intron 40.0%
! TCAGATCCGATTACTGATTG+TGG - Chr2:80817069-80817088 MsG0280011194.01.T01:intron 40.0%
! TGAGGTTCTCAAAGTTTTGC+AGG - Chr2:80814359-80814378 MsG0280011194.01.T01:intron 40.0%
! TGATGATGATGACGATGAAG+AGG - Chr2:80816947-80816966 MsG0280011194.01.T01:intron 40.0%
! TTTTGTGTCAAGCACTTTCC+TGG + Chr2:80815919-80815938 None:intergenic 40.0%
!! AGGAAAGTGCTTGACACAAA+AGG - Chr2:80815918-80815937 MsG0280011194.01.T01:intron 40.0%
!! TTGGTATCCGTTAGTCTTGA+TGG - Chr2:80816276-80816295 MsG0280011194.01.T01:intron 40.0%
AAACTCCAGCTTGAAACCCT+TGG + Chr2:80815701-80815720 None:intergenic 45.0%
AACTCCAGCTTGAAACCCTT+GGG + Chr2:80815700-80815719 None:intergenic 45.0%
ATAAGCTTCAGCTCTTGGCT+GGG - Chr2:80814286-80814305 MsG0280011194.01.T01:intron 45.0%
ATGATGAGGAGGAAGACGAA+GGG - Chr2:80816982-80817001 MsG0280011194.01.T01:intron 45.0%
CATGCAAGAAACAGAGGCAA+TGG + Chr2:80816134-80816153 None:intergenic 45.0%
CCGAATGCCATCAAGACTAA+CGG + Chr2:80816286-80816305 None:intergenic 45.0%
CCTATCCATGCAAGAAACAG+AGG + Chr2:80816140-80816159 None:intergenic 45.0%
GAAGAAAGCAAAGCTGCTGA+AGG - Chr2:80815377-80815396 MsG0280011194.01.T01:CDS 45.0%
GCACAGGCACTTATCATGAT+AGG + Chr2:80815184-80815203 None:intergenic 45.0%
GCAGAGCGAACATGATGAAT+TGG - Chr2:80814857-80814876 MsG0280011194.01.T01:intron 45.0%
GGAAGAACAAGAGCAAGGTA+GGG - Chr2:80817009-80817028 MsG0280011194.01.T01:CDS 45.0%
GGATGAGGATGAAGATGATG+AGG - Chr2:80816968-80816987 MsG0280011194.01.T01:intron 45.0%
GTAGGATAAGCTTCAGCTCT+TGG - Chr2:80814281-80814300 MsG0280011194.01.T01:intron 45.0%
GTTCCACTTGATCACAAGCA+TGG + Chr2:80816520-80816539 None:intergenic 45.0%
TAATGTCAGGCAACGTGTTG+AGG - Chr2:80814341-80814360 MsG0280011194.01.T01:intron 45.0%
TCAACACGTTGCCTGACATT+AGG + Chr2:80814342-80814361 None:intergenic 45.0%
TCATGCTGTGTCATGGTTCA+CGG - Chr2:80816848-80816867 MsG0280011194.01.T01:intron 45.0%
TGAGGATGAAGATGATGAGG+AGG - Chr2:80816971-80816990 MsG0280011194.01.T01:intron 45.0%
TGATGATAGATGTCACCGGA+AGG - Chr2:80816732-80816751 MsG0280011194.01.T01:CDS 45.0%
TTAACTGCAGAGTGGTAGCA+AGG - Chr2:80817171-80817190 MsG0280011194.01.T01:intron 45.0%
! CATTGCCTCTGTTTCTTGCA+TGG - Chr2:80816132-80816151 MsG0280011194.01.T01:CDS 45.0%
! CCTCTGTTTCTTGCATGGAT+AGG - Chr2:80816137-80816156 MsG0280011194.01.T01:CDS 45.0%
! CTGGTGATGATAGATGTCAC+CGG - Chr2:80816728-80816747 MsG0280011194.01.T01:CDS 45.0%
! TGATGACGATGAAGAGGATG+AGG - Chr2:80816953-80816972 MsG0280011194.01.T01:intron 45.0%
! TTAGCCTGTTTTCGTGTGTG+TGG - Chr2:80814418-80814437 MsG0280011194.01.T01:intron 45.0%
!! AAAAATGGTGGGGGCATTTG+CGG - Chr2:80816702-80816721 MsG0280011194.01.T01:intron 45.0%
!! CATGTATCGGTGTCAGACAT+AGG + Chr2:80814622-80814641 None:intergenic 45.0%
!! CCGTTAGTCTTGATGGCATT+CGG - Chr2:80816283-80816302 MsG0280011194.01.T01:intron 45.0%
!! TAGAAGTGTCGGTGCTACAA+AGG + Chr2:80814586-80814605 None:intergenic 45.0%
!! AAAAAGAAAAAAAAAAAAAT+TGG - Chr2:80816644-80816663 MsG0280011194.01.T01:intron 5.0%
CAATCCCAAGGGTTTCAAGC+TGG - Chr2:80815693-80815712 MsG0280011194.01.T01:intron 50.0%
CATGCTGTGTCATGGTTCAC+GGG - Chr2:80816849-80816868 MsG0280011194.01.T01:intron 50.0%
GAAATTGATGTTACCGGCGG+CGG + Chr2:80813881-80813900 None:intergenic 50.0%
GAAGGGGAAGAACAAGAGCA+AGG - Chr2:80817004-80817023 MsG0280011194.01.T01:intron 50.0%
GAGAAAGGAGTCCCTAGCTT+TGG - Chr2:80815486-80815505 MsG0280011194.01.T01:intron 50.0%
GATAAGCTTCAGCTCTTGGC+TGG - Chr2:80814285-80814304 MsG0280011194.01.T01:intron 50.0%
GATCACTGAACGTGATGAGG+AGG - Chr2:80815630-80815649 MsG0280011194.01.T01:intron 50.0%
GATGATGAGGAGGAAGACGA+AGG - Chr2:80816981-80817000 MsG0280011194.01.T01:intron 50.0%
GCAAAACTGCTTATGGCGCA+TGG - Chr2:80816453-80816472 MsG0280011194.01.T01:intron 50.0%
GGGAAGAACAAGAGCAAGGT+AGG - Chr2:80817008-80817027 MsG0280011194.01.T01:CDS 50.0%
GTCATCATCCTCGGGAATCT+GGG + Chr2:80816037-80816056 None:intergenic 50.0%
GTGAACCATGACACAGCATG+AGG + Chr2:80816849-80816868 None:intergenic 50.0%
GTGCCATGCTTGTGATCAAG+TGG - Chr2:80816514-80816533 MsG0280011194.01.T01:intron 50.0%
TAAGCTTCAGCTCTTGGCTG+GGG - Chr2:80814287-80814306 MsG0280011194.01.T01:intron 50.0%
TCATCATCCTCGGGAATCTG+GGG + Chr2:80816036-80816055 None:intergenic 50.0%
TTAGACACTACGGGCCTGTT+TGG + Chr2:80815221-80815240 None:intergenic 50.0%
TTTAGAGGAGCGAGCTCAGT+TGG - Chr2:80814890-80814909 MsG0280011194.01.T01:intron 50.0%
! ACTGCTGCTTGCAATCAGCA+GGG + Chr2:80817228-80817247 None:intergenic 50.0%
! GCACCGACACTTCTAATCGA+AGG - Chr2:80814591-80814610 MsG0280011194.01.T01:intron 50.0%
! TACTGCTGCTTGCAATCAGC+AGG + Chr2:80817229-80817248 None:intergenic 50.0%
ACGGAAATTGAGTGGCACCC+AGG - Chr2:80815898-80815917 MsG0280011194.01.T01:intron 55.0%
AGGAGGAAGACGAAGGGAAG+GGG - Chr2:80816988-80817007 MsG0280011194.01.T01:intron 55.0%
AGTGGTAGCAAGGCCAAACC+TGG - Chr2:80817181-80817200 MsG0280011194.01.T01:intron 55.0%
CGTCATCATCCTCGGGAATC+TGG + Chr2:80816038-80816057 None:intergenic 55.0%
GAGGAGGAAGACGAAGGGAA+GGG - Chr2:80816987-80817006 MsG0280011194.01.T01:intron 55.0%
GCAGCCACACACACGAAAAC+AGG + Chr2:80814425-80814444 None:intergenic 55.0%
TGAGGAGGAAGACGAAGGGA+AGG - Chr2:80816986-80817005 MsG0280011194.01.T01:intron 55.0%
TGGGGATCGTGTGGATGTCA+TGG - Chr2:80814305-80814324 MsG0280011194.01.T01:intron 55.0%
TGTGTCATGGTTCACGGGAG+AGG - Chr2:80816854-80816873 MsG0280011194.01.T01:intron 55.0%
! CTCAGTGCTGAAGATCGTGC+TGG - Chr2:80814049-80814068 MsG0280011194.01.T01:intron 55.0%
!! GGGAAGATCGGCGGATTGAT+CGG + Chr2:80813854-80813873 None:intergenic 55.0%
CATCCTCGGGAATCTGGGGA+GGG + Chr2:80816032-80816051 None:intergenic 60.0%
TCATCCTCGGGAATCTGGGG+AGG + Chr2:80816033-80816052 None:intergenic 60.0%
!! GTGGGGGCATTTGCGGAATC+TGG - Chr2:80816709-80816728 MsG0280011194.01.T01:intron 60.0%
CAACCCTCCCCAGATTCCCG+AGG - Chr2:80816026-80816045 MsG0280011194.01.T01:intron 65.0%
GCTCTTGGCTGGGGATCGTG+TGG - Chr2:80814296-80814315 MsG0280011194.01.T01:intron 65.0%
ATGTTACCGGCGGCGGCAGC+GGG + Chr2:80813874-80813893 None:intergenic 70.0%
GATGTTACCGGCGGCGGCAG+CGG + Chr2:80813875-80813894 None:intergenic 70.0%
GATCTTCCCGCTGCCGCCGC+CGG - Chr2:80813865-80813884 MsG0280011194.01.T01:CDS 75.0%
GGCGGCAGCGGGAAGATCGG+CGG + Chr2:80813863-80813882 None:intergenic 75.0%
GGCGGCGGCAGCGGGAAGAT+CGG + Chr2:80813866-80813885 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 80813847 80817249 80813847 ID=MsG0280011194.01;Name=MsG0280011194.01
Chr2 mRNA 80813847 80817249 80813847 ID=MsG0280011194.01.T01;Parent=MsG0280011194.01;Name=MsG0280011194.01.T01;_AED=0.50;_eAED=0.56;_QI=0|0|0|1|0.77|0.8|10|0|353
Chr2 exon 80817210 80817249 80817210 ID=MsG0280011194.01.T01:exon:28719;Parent=MsG0280011194.01.T01
Chr2 exon 80817006 80817079 80817006 ID=MsG0280011194.01.T01:exon:28718;Parent=MsG0280011194.01.T01
Chr2 exon 80816716 80816811 80816716 ID=MsG0280011194.01.T01:exon:28717;Parent=MsG0280011194.01.T01
Chr2 exon 80816087 80816235 80816087 ID=MsG0280011194.01.T01:exon:28716;Parent=MsG0280011194.01.T01
Chr2 exon 80815698 80815782 80815698 ID=MsG0280011194.01.T01:exon:28715;Parent=MsG0280011194.01.T01
Chr2 exon 80815344 80815465 80815344 ID=MsG0280011194.01.T01:exon:28714;Parent=MsG0280011194.01.T01
Chr2 exon 80815022 80815199 80815022 ID=MsG0280011194.01.T01:exon:28713;Parent=MsG0280011194.01.T01
Chr2 exon 80814894 80814937 80814894 ID=MsG0280011194.01.T01:exon:28712;Parent=MsG0280011194.01.T01
Chr2 exon 80814071 80814275 80814071 ID=MsG0280011194.01.T01:exon:28711;Parent=MsG0280011194.01.T01
Chr2 exon 80813847 80813915 80813847 ID=MsG0280011194.01.T01:exon:28710;Parent=MsG0280011194.01.T01
Chr2 CDS 80817210 80817249 80817210 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Chr2 CDS 80817006 80817079 80817006 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Chr2 CDS 80816716 80816811 80816716 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Chr2 CDS 80816087 80816235 80816087 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Chr2 CDS 80815698 80815782 80815698 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Chr2 CDS 80815344 80815465 80815344 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Chr2 CDS 80815022 80815199 80815022 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Chr2 CDS 80814894 80814937 80814894 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Chr2 CDS 80814071 80814275 80814071 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Chr2 CDS 80813847 80813915 80813847 ID=MsG0280011194.01.T01:cds;Parent=MsG0280011194.01.T01
Gene Sequence

>MsG0280011194.01.T01

ATGTCCGATCAATCCGCCGATCTTCCCGCTGCCGCCGCCGGTCTAAAATACTGTTGCTTTTTTCTTGTAGCTCTCAGTGCTGAAGATCGTGCTGGTCTCGTCAATGCACTCAAAGATAAGCTTCAGCTCTTGGCTGGGGATCGTGTGGATGTCATGGAAACACTTTCGCCTAATGTCAGGCAACGTGTTGAGGTTCTCAAAGTTTTGCAGAGCGAACATGATGAATTGGAGAGTAAGTTTTTAGAGGAGCGAGCTCAGTTGGAAGCTAAATACCAGAAGCTTTATGAACCACTTTACACAAAGGTGGTGTTTGGATTGCCTTATTTTAGCTTATCTATTGGCATACATTTTGCGACACTAGGTACGAGATTGTCAATGGTGTTATTGACGTTGAAGGAATTACAAACGAAGCAGGACAGGGAGAAGAAAGCAAAGCTGCTGAAGATCACTGAACGTGATGAGGAGGCTCTCAAGTATCTCAAAGATATCAAGTGGAGTAAACTTGACAATCCCAAGGGTTTCAAGCTGGAGTTTTATTTTGATTCTAATCCTTATTTCCAAAATCCAACGGAAATTGAGTGGCACCCAGGAAAGTGCTTGACACAAAAGGTTATGAAGAAGAGGCCAAAGAAAGGATCAAAAAATGCCAAACCAATCATAAAAACAGAAAAATGTGATAGTTTCTTCAACATTTTCAACCCTCCCCAGATTCCCGAGGATGATGACGAAATTGATGACGATGTTGTTGAAGAGCTTCAAAATTTAATGGAGCATGATTATGATATTGGTTCTACAATCCGAGACAAAATTATTCCTCATGCTGTGTCATGGTTCACGGGAGAGGCTGTAGAGAGTGATTTTGACGATATAGAAGTAGATGAAGATGATGAAGACAGAGATGAAGATGAAGAGGATGATGATGATGACGATGAAGAGGATGAGGATGAAGATGATGAGGAGGAAGACGAAGGGAAGGGGAAGAACAAGAGCAAGAGTGGTAGCAAGGCCAAACCTGGTAAAGATCAGTCAACTGAGCGCCCTGCTGATTGCAAGCAGCAGTAG
Protein sequence

>MsG0280011194.01.T01

MSDQSADLPAAAAGLKYCCFFLVALSAEDRAGLVNALKDKLQLLAGDRVDVMETLSPNVRQRVEVLKVLQSEHDELESKFLEERAQLEAKYQKLYEPLYTKVVFGLPYFSLSIGIHFATLGTRLSMVLLTLKELQTKQDREKKAKLLKITERDEEALKYLKDIKWSKLDNPKGFKLEFYFDSNPYFQNPTEIEWHPGKCLTQKVMKKRPKKGSKNAKPIIKTEKCDSFFNIFNPPQIPEDDDEIDDDVVEELQNLMEHDYDIGSTIRDKIIPHAVSWFTGEAVESDFDDIEVDEDDEDRDEDEEDDDDDDEEDEDEDDEEEDEGKGKNKSKSGSKAKPGKDQSTERPADCKQQ*