AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018603.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018603.01.T01 MTR_4g015180 100.000 74 0 0 1 74 417 490 1.31e-47 157
MsG0480018603.01.T01 MTR_5g081960 80.282 71 14 0 1 71 416 486 5.78e-37 129
MsG0480018603.01.T01 MTR_3g070170 71.831 71 20 0 1 71 400 470 7.72e-33 117
MsG0480018603.01.T01 MTR_3g102020 65.672 67 23 0 1 67 381 447 3.98e-26 99.0
MsG0480018603.01.T01 MTR_3g101960 65.152 66 23 0 1 66 380 445 8.17e-26 98.2
MsG0480018603.01.T01 MTR_3g102010 61.972 71 27 0 1 71 381 451 1.39e-25 97.8
MsG0480018603.01.T01 MTR_3g101970 55.714 70 31 0 1 70 367 436 7.06e-22 87.4
MsG0480018603.01.T01 MTR_3g101900 53.521 71 33 0 1 71 378 448 1.55e-19 80.9
MsG0480018603.01.T01 MTR_4g094835 56.923 65 27 1 3 67 379 442 2.15e-19 80.5
MsG0480018603.01.T01 MTR_6g045553 54.286 70 32 0 1 70 405 474 2.55e-19 80.1
MsG0480018603.01.T01 MTR_4g094825 55.224 67 29 1 1 67 322 387 3.03e-19 79.7
MsG0480018603.01.T01 MTR_0190s0020 52.113 71 33 1 1 71 373 442 1.76e-17 75.1
MsG0480018603.01.T01 MTR_4g094838 53.846 65 29 1 3 67 365 428 1.83e-17 75.1
MsG0480018603.01.T01 MTR_4g094842 51.515 66 31 1 3 68 381 445 1.29e-16 72.4
MsG0480018603.01.T01 MTR_4g094832 53.846 65 29 1 3 67 363 426 3.31e-16 71.2
MsG0480018603.01.T01 MTR_8g041770 50.000 70 34 1 3 72 374 442 5.21e-16 70.9
MsG0480018603.01.T01 MTR_3g101930 48.571 70 35 1 1 70 386 454 1.07e-15 70.1
MsG0480018603.01.T01 MTR_4g022800 50.000 72 33 2 1 72 375 443 1.36e-15 69.7
MsG0480018603.01.T01 MTR_4g022825 48.571 70 33 2 1 70 373 439 1.71e-14 66.6
MsG0480018603.01.T01 MTR_3g088420 45.714 70 35 2 3 72 368 434 1.16e-13 64.3
MsG0480018603.01.T01 MTR_4g022720 45.833 72 36 2 1 72 368 436 1.65e-13 63.9
MsG0480018603.01.T01 MTR_4g022670 44.444 72 37 2 1 72 374 442 5.36e-13 62.4
MsG0480018603.01.T01 MTR_7g026860 44.444 72 37 2 1 72 371 439 4.82e-12 59.7
MsG0480018603.01.T01 MTR_8g040780 53.226 62 26 2 4 63 164 224 6.68e-12 58.5
MsG0480018603.01.T01 MTR_3g026310 44.286 70 36 2 3 72 383 449 4.80e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018603.01.T01 AT5G01210 85.135 74 11 0 1 74 399 472 2.91e-40 137
MsG0480018603.01.T01 AT2G39980 83.333 72 12 0 1 72 410 481 1.26e-38 133
MsG0480018603.01.T01 AT5G42830 60.563 71 28 0 1 71 379 449 3.02e-23 91.3
MsG0480018603.01.T01 AT5G07850 60.563 71 28 0 1 71 383 453 8.48e-23 90.1
MsG0480018603.01.T01 AT5G07870 57.746 71 30 0 1 71 391 461 1.49e-22 89.4
MsG0480018603.01.T01 AT5G07860 60.000 70 28 0 1 70 379 448 6.22e-22 87.4
MsG0480018603.01.T01 AT3G50270 46.479 71 37 1 1 71 379 448 9.49e-17 73.2
MsG0480018603.01.T01 AT3G50280 48.529 68 34 1 1 68 244 310 4.54e-16 70.5
MsG0480018603.01.T01 AT3G50280 48.529 68 34 1 1 68 374 440 6.51e-16 70.5
MsG0480018603.01.T01 AT5G67150 43.836 73 40 1 1 73 377 448 1.40e-15 69.7
MsG0480018603.01.T01 AT5G23940 41.791 67 39 0 1 67 382 448 8.44e-13 61.6

Find 13 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTATGTATGATAATGATTT+TGG 0.104858 4:+7239840 MsG0480018603.01.T01:CDS
GGGGTATACCTTTGGCTATA+AGG 0.379202 4:+7239865 MsG0480018603.01.T01:CDS
TTGAAACCTGAGACTATGTT+TGG 0.415070 4:+7239972 MsG0480018603.01.T01:CDS
GGTAAAAGTAATAAATTTGA+TGG 0.432414 4:+7239891 MsG0480018603.01.T01:CDS
TCAAGTCCAAACATAGTCTC+AGG 0.460721 4:-7239978 None:intergenic
TTTCCTGGTAGAGAAGGAAA+TGG 0.481363 4:+7239927 MsG0480018603.01.T01:CDS
CTCCCATTTCCTTCTCTACC+AGG 0.502041 4:-7239930 None:intergenic
TTCCTGGTAGAGAAGGAAAT+GGG 0.518635 4:+7239928 MsG0480018603.01.T01:CDS
CCAAAATCATTATCATACAT+AGG 0.572772 4:-7239840 None:intergenic
TTACCACTCCTTATAGCCAA+AGG 0.582020 4:-7239873 None:intergenic
GTTTGGACTTGAAAATGATG+TGG 0.601021 4:+7239989 MsG0480018603.01.T01:CDS
ATACCTTTGGCTATAAGGAG+TGG 0.628958 4:+7239870 MsG0480018603.01.T01:CDS
TTATCATACATAGGAAAACG+TGG 0.723507 4:-7239831 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGTAAAAGTAATAAATTTGA+TGG + Chr4:7239891-7239910 MsG0480018603.01.T01:CDS 20.0%
!!! CTATGTATGATAATGATTTT+GGG + Chr4:7239841-7239860 MsG0480018603.01.T01:CDS 20.0%
! CCAAAATCATTATCATACAT+AGG - Chr4:7239843-7239862 None:intergenic 25.0%
!!! CCTATGTATGATAATGATTT+TGG + Chr4:7239840-7239859 MsG0480018603.01.T01:CDS 25.0%
TTATCATACATAGGAAAACG+TGG - Chr4:7239834-7239853 None:intergenic 30.0%
!! GTATGATAATGATTTTGGGT+GGG + Chr4:7239845-7239864 MsG0480018603.01.T01:CDS 30.0%
!! TATGATAATGATTTTGGGTG+GGG + Chr4:7239846-7239865 MsG0480018603.01.T01:CDS 30.0%
!! TGTATGATAATGATTTTGGG+TGG + Chr4:7239844-7239863 MsG0480018603.01.T01:CDS 30.0%
GTTTGGACTTGAAAATGATG+TGG + Chr4:7239989-7240008 MsG0480018603.01.T01:CDS 35.0%
TTGAAACCTGAGACTATGTT+TGG + Chr4:7239972-7239991 MsG0480018603.01.T01:CDS 35.0%
! GGTAAAATTTCAGCTTTTCC+TGG + Chr4:7239912-7239931 MsG0480018603.01.T01:CDS 35.0%
ATACCTTTGGCTATAAGGAG+TGG + Chr4:7239870-7239889 MsG0480018603.01.T01:CDS 40.0%
TCAAGTCCAAACATAGTCTC+AGG - Chr4:7239981-7240000 None:intergenic 40.0%
TTACCACTCCTTATAGCCAA+AGG - Chr4:7239876-7239895 None:intergenic 40.0%
TTCCTGGTAGAGAAGGAAAT+GGG + Chr4:7239928-7239947 MsG0480018603.01.T01:CDS 40.0%
TTTCCTGGTAGAGAAGGAAA+TGG + Chr4:7239927-7239946 MsG0480018603.01.T01:CDS 40.0%
GGGGTATACCTTTGGCTATA+AGG + Chr4:7239865-7239884 MsG0480018603.01.T01:CDS 45.0%
! TCAGCTTTTCCTGGTAGAGA+AGG + Chr4:7239921-7239940 MsG0480018603.01.T01:CDS 45.0%
!! TTTTGGGTGGGGTATACCTT+TGG + Chr4:7239857-7239876 MsG0480018603.01.T01:CDS 45.0%
CTCCCATTTCCTTCTCTACC+AGG - Chr4:7239933-7239952 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 7239819 7240043 7239819 ID=MsG0480018603.01;Name=MsG0480018603.01
Chr4 mRNA 7239819 7240043 7239819 ID=MsG0480018603.01.T01;Parent=MsG0480018603.01;Name=MsG0480018603.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|74
Chr4 exon 7239819 7240043 7239819 ID=MsG0480018603.01.T01:exon:1780;Parent=MsG0480018603.01.T01
Chr4 CDS 7239819 7240043 7239819 ID=MsG0480018603.01.T01:cds;Parent=MsG0480018603.01.T01
Gene Sequence

>MsG0480018603.01.T01

ATGGGTAGTTCACCACGTTTTCCTATGTATGATAATGATTTTGGGTGGGGTATACCTTTGGCTATAAGGAGTGGTAAAAGTAATAAATTTGATGGTAAAATTTCAGCTTTTCCTGGTAGAGAAGGAAATGGGAGTGTTGATCTTGAAGTTGTTTTGAAACCTGAGACTATGTTTGGACTTGAAAATGATGTGGAATTTATGCAGTATGTTACTGATGTAGTGTGA

Protein sequence

>MsG0480018603.01.T01

MGSSPRFPMYDNDFGWGIPLAIRSGKSNKFDGKISAFPGREGNGSVDLEVVLKPETMFGLENDVEFMQYVTDVV*