AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023361.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023361.01.T01 MTR_8g091600 97.857 140 3 0 1 140 52 191 4.72e-82 239
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTCTCTCCTTTGGTTAT+AGG 0.171653 4:+84189457 None:intergenic
CTTTGAGTTGCTCTTGTTGT+TGG 0.256568 4:+84189288 None:intergenic
ATGTTGCTTGTCTTGACTTT+GGG 0.296941 4:+84189432 None:intergenic
CATGTTGCTTGTCTTGACTT+TGG 0.323819 4:+84189431 None:intergenic
GCCCCACCAGCACCACCTTC+AGG 0.352873 4:+84189259 None:intergenic
GCTGGTGGGGCAGATGAAAA+TGG 0.354321 4:-84189247 MsG0480023361.01.T01:CDS
GACGATGGAGATGGTGCATT+TGG 0.394570 4:-84189364 MsG0480023361.01.T01:CDS
TCCTGAAGGTGGTGCTGGTG+GGG 0.411941 4:-84189260 MsG0480023361.01.T01:CDS
GCTCCTGAAGGTGGTGCTGG+TGG 0.414345 4:-84189262 MsG0480023361.01.T01:CDS
GGAGAAGAGGACGAGGAGGA+AGG 0.430641 4:-84189142 MsG0480023361.01.T01:CDS
CTCCTGAAGGTGGTGCTGGT+GGG 0.473479 4:-84189261 MsG0480023361.01.T01:CDS
GAGATGGTGCATTTGGAGAA+GGG 0.480248 4:-84189357 MsG0480023361.01.T01:CDS
AGAAGACAATGAAGAGGAAG+AGG 0.484704 4:-84189110 MsG0480023361.01.T01:CDS
CTTTGCAGCCACCAAAGAAA+AGG 0.492171 4:-84189069 MsG0480023361.01.T01:CDS
CCTCATCGTCATCCTCGTCT+TGG 0.494393 4:+84189194 None:intergenic
GGATGACGACGATGAGGAAG+AGG 0.500878 4:-84189167 MsG0480023361.01.T01:CDS
AGAGGGTGGAGAAGAGGACG+AGG 0.509661 4:-84189149 MsG0480023361.01.T01:CDS
AAAGCTCCTGAAGGTGGTGC+TGG 0.510556 4:-84189265 MsG0480023361.01.T01:CDS
AGACAATGAAGAGGAAGAGG+AGG 0.514365 4:-84189107 MsG0480023361.01.T01:CDS
GGAAGAGGAGGATGAAGATG+AGG 0.522929 4:-84189095 MsG0480023361.01.T01:CDS
GATGAATTGTCATCTGAAGA+TGG 0.523962 4:-84189331 MsG0480023361.01.T01:CDS
CATGATGCTGAAGGAGAAGA+TGG 0.525643 4:-84189412 MsG0480023361.01.T01:CDS
GGAGATGGTGCATTTGGAGA+AGG 0.529378 4:-84189358 MsG0480023361.01.T01:CDS
TCTGAAGATGGAGCTGGATA+CGG 0.537126 4:-84189319 MsG0480023361.01.T01:CDS
GGGTGGAGAAGAGGACGAGG+AGG 0.543759 4:-84189146 MsG0480023361.01.T01:CDS
CGAGGATGACGATGAGGACG+AGG 0.544157 4:-84189188 MsG0480023361.01.T01:CDS
TGAGGAAGAGGGTGGAGAAG+AGG 0.553351 4:-84189155 MsG0480023361.01.T01:CDS
TTGTCATCTGAAGATGGAGC+TGG 0.560808 4:-84189325 MsG0480023361.01.T01:CDS
GGCAGATGAAAATGGAGAAG+AGG 0.564151 4:-84189239 MsG0480023361.01.T01:CDS
AGATGGTGCATTTGGAGAAG+GGG 0.569589 4:-84189356 MsG0480023361.01.T01:CDS
GAAGAGGAAGATGATGAAGA+CGG 0.572055 4:-84189223 MsG0480023361.01.T01:CDS
TGATGAAGAAGACAATGAAG+AGG 0.577160 4:-84189116 MsG0480023361.01.T01:CDS
AACTCAAAGAAAGCTCCTGA+AGG 0.580770 4:-84189274 MsG0480023361.01.T01:CDS
GAAGAAGATGACGATGGAGA+TGG 0.589363 4:-84189373 MsG0480023361.01.T01:CDS
TCAAAGAAAGCTCCTGAAGG+TGG 0.613002 4:-84189271 MsG0480023361.01.T01:CDS
GACAAGCAACATGATGCTGA+AGG 0.614183 4:-84189421 MsG0480023361.01.T01:CDS
GGACGAGGATGACGACGATG+AGG 0.626052 4:-84189173 MsG0480023361.01.T01:CDS
ACCGACATGCCTATAACCAA+AGG 0.632185 4:-84189466 None:intergenic
GATGACGACGATGAGGAAGA+GGG 0.650173 4:-84189166 MsG0480023361.01.T01:CDS
GACGACGATGAGGAAGAGGG+TGG 0.671206 4:-84189163 MsG0480023361.01.T01:CDS
GAAGACGAAGAAGATGACGA+TGG 0.695068 4:-84189379 MsG0480023361.01.T01:CDS
CCAAGACGAGGATGACGATG+AGG 0.711949 4:-84189194 MsG0480023361.01.T01:CDS
AGACGGTGATGACCAAGACG+AGG 0.728787 4:-84189206 MsG0480023361.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GATGAATTGTCATCTGAAGA+TGG - Chr4:84189189-84189208 MsG0480023361.01.T01:CDS 35.0%
TGATGAAGAAGACAATGAAG+AGG - Chr4:84189404-84189423 MsG0480023361.01.T01:CDS 35.0%
TTACTCTCTCCTTTGGTTAT+AGG + Chr4:84189066-84189085 None:intergenic 35.0%
! ATGTTGCTTGTCTTGACTTT+GGG + Chr4:84189091-84189110 None:intergenic 35.0%
AACTCAAAGAAAGCTCCTGA+AGG - Chr4:84189246-84189265 MsG0480023361.01.T01:CDS 40.0%
AGAAGACAATGAAGAGGAAG+AGG - Chr4:84189410-84189429 MsG0480023361.01.T01:CDS 40.0%
GAAGAGGAAGATGATGAAGA+CGG - Chr4:84189297-84189316 MsG0480023361.01.T01:CDS 40.0%
! CACTTCTTCCTTTTCTTTGG+TGG + Chr4:84189462-84189481 None:intergenic 40.0%
! CATGTTGCTTGTCTTGACTT+TGG + Chr4:84189092-84189111 None:intergenic 40.0%
! TTGGGTTTTACTCTCTCCTT+TGG + Chr4:84189073-84189092 None:intergenic 40.0%
!! CTTTGAGTTGCTCTTGTTGT+TGG + Chr4:84189235-84189254 None:intergenic 40.0%
AGACAATGAAGAGGAAGAGG+AGG - Chr4:84189413-84189432 MsG0480023361.01.T01:CDS 45.0%
AGATGGTGCATTTGGAGAAG+GGG - Chr4:84189164-84189183 MsG0480023361.01.T01:CDS 45.0%
CATGATGCTGAAGGAGAAGA+TGG - Chr4:84189108-84189127 MsG0480023361.01.T01:CDS 45.0%
CTTTGCAGCCACCAAAGAAA+AGG - Chr4:84189451-84189470 MsG0480023361.01.T01:CDS 45.0%
GAAGAAGATGACGATGGAGA+TGG - Chr4:84189147-84189166 MsG0480023361.01.T01:CDS 45.0%
GAAGACGAAGAAGATGACGA+TGG - Chr4:84189141-84189160 MsG0480023361.01.T01:CDS 45.0%
GACAAGCAACATGATGCTGA+AGG - Chr4:84189099-84189118 MsG0480023361.01.T01:CDS 45.0%
GAGATGGTGCATTTGGAGAA+GGG - Chr4:84189163-84189182 MsG0480023361.01.T01:CDS 45.0%
GGCAGATGAAAATGGAGAAG+AGG - Chr4:84189281-84189300 MsG0480023361.01.T01:CDS 45.0%
TCAAAGAAAGCTCCTGAAGG+TGG - Chr4:84189249-84189268 MsG0480023361.01.T01:CDS 45.0%
TCTGAAGATGGAGCTGGATA+CGG - Chr4:84189201-84189220 MsG0480023361.01.T01:CDS 45.0%
TTGTCATCTGAAGATGGAGC+TGG - Chr4:84189195-84189214 MsG0480023361.01.T01:CDS 45.0%
GACGATGGAGATGGTGCATT+TGG - Chr4:84189156-84189175 MsG0480023361.01.T01:CDS 50.0%
GATGACGACGATGAGGAAGA+GGG - Chr4:84189354-84189373 MsG0480023361.01.T01:CDS 50.0%
GGAAGAGGAGGATGAAGATG+AGG - Chr4:84189425-84189444 MsG0480023361.01.T01:CDS 50.0%
GGAGATGGTGCATTTGGAGA+AGG - Chr4:84189162-84189181 MsG0480023361.01.T01:CDS 50.0%
AAAGCTCCTGAAGGTGGTGC+TGG - Chr4:84189255-84189274 MsG0480023361.01.T01:CDS 55.0%
AGACGGTGATGACCAAGACG+AGG - Chr4:84189314-84189333 MsG0480023361.01.T01:CDS 55.0%
CCAAGACGAGGATGACGATG+AGG - Chr4:84189326-84189345 MsG0480023361.01.T01:CDS 55.0%
CCTCATCGTCATCCTCGTCT+TGG + Chr4:84189329-84189348 None:intergenic 55.0%
GCTGGTGGGGCAGATGAAAA+TGG - Chr4:84189273-84189292 MsG0480023361.01.T01:CDS 55.0%
GGATGACGACGATGAGGAAG+AGG - Chr4:84189353-84189372 MsG0480023361.01.T01:CDS 55.0%
TGAGGAAGAGGGTGGAGAAG+AGG - Chr4:84189365-84189384 MsG0480023361.01.T01:CDS 55.0%
AGAGGGTGGAGAAGAGGACG+AGG - Chr4:84189371-84189390 MsG0480023361.01.T01:CDS 60.0%
CGAGGATGACGATGAGGACG+AGG - Chr4:84189332-84189351 MsG0480023361.01.T01:CDS 60.0%
GACGACGATGAGGAAGAGGG+TGG - Chr4:84189357-84189376 MsG0480023361.01.T01:CDS 60.0%
GGACGAGGATGACGACGATG+AGG - Chr4:84189347-84189366 MsG0480023361.01.T01:CDS 60.0%
GGAGAAGAGGACGAGGAGGA+AGG - Chr4:84189378-84189397 MsG0480023361.01.T01:CDS 60.0%
!! CTCCTGAAGGTGGTGCTGGT+GGG - Chr4:84189259-84189278 MsG0480023361.01.T01:CDS 60.0%
!! TCCTGAAGGTGGTGCTGGTG+GGG - Chr4:84189260-84189279 MsG0480023361.01.T01:CDS 60.0%
GGGTGGAGAAGAGGACGAGG+AGG - Chr4:84189374-84189393 MsG0480023361.01.T01:CDS 65.0%
! GCTCCTGAAGGTGGTGCTGG+TGG - Chr4:84189258-84189277 MsG0480023361.01.T01:CDS 65.0%
GCCCCACCAGCACCACCTTC+AGG + Chr4:84189264-84189283 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 84189060 84189482 84189060 ID=MsG0480023361.01;Name=MsG0480023361.01
Chr4 mRNA 84189060 84189482 84189060 ID=MsG0480023361.01.T01;Parent=MsG0480023361.01;Name=MsG0480023361.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|140
Chr4 exon 84189060 84189482 84189060 ID=MsG0480023361.01.T01:exon:12444;Parent=MsG0480023361.01.T01
Chr4 CDS 84189060 84189482 84189060 ID=MsG0480023361.01.T01:cds;Parent=MsG0480023361.01.T01
Gene Sequence

>MsG0480023361.01.T01

ATGCCTATAACCAAAGGAGAGAGTAAAACCCAAAGTCAAGACAAGCAACATGATGCTGAAGGAGAAGATGGCAATGATGATGAAGACGAAGAAGATGACGATGGAGATGGTGCATTTGGAGAAGGGGAAGATGAATTGTCATCTGAAGATGGAGCTGGATACGGCAACAATTCCAACAACAAGAGCAACTCAAAGAAAGCTCCTGAAGGTGGTGCTGGTGGGGCAGATGAAAATGGAGAAGAGGAAGATGATGAAGACGGTGATGACCAAGACGAGGATGACGATGAGGACGAGGATGACGACGATGAGGAAGAGGGTGGAGAAGAGGACGAGGAGGAAGGTGTTGATGAAGAAGACAATGAAGAGGAAGAGGAGGATGAAGATGAGGAAGCTTTGCAGCCACCAAAGAAAAGGAAGAAGTGA

Protein sequence

>MsG0480023361.01.T01

MPITKGESKTQSQDKQHDAEGEDGNDDEDEEDDDGDGAFGEGEDELSSEDGAGYGNNSNNKSNSKKAPEGGAGGADENGEEEDDEDGDDQDEDDDEDEDDDDEEEGGEEDEEEGVDEEDNEEEEEDEDEEALQPPKKRKK*