AlfalfaGEDB | MsG0380012366.01

M. sativa cultivar ZhongmuNo.1 / MsG0380012366.01

KEGG: -
GO: -
PFAM: PF07145

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012366.01.T01	AFK42480.1	96.296	54	2	0	1	54	1	54	6.89E-31	115

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012366.01.T01	Q39096	67.925	53	17	0	1	53	1	53	1.17E-22	87.4

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012366.01.T01	I3SQD8	96.296	54	2	0	1	54	1	54	3.29e-31	115

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048699.01	MsG0380012366.01	-0.827387	1.609204e-54	1.551451e-51
MsG0180000337.01	MsG0380012366.01	0.800518	1.388066e-48	6.476345e-46
MsG0180002232.01	MsG0380012366.01	-0.808874	2.499370e-50	1.445451e-47
MsG0180004761.01	MsG0380012366.01	-0.841430	4.776016e-58	7.016385e-55
MsG0380012366.01	MsG0380013942.01	-0.804332	2.272561e-49	1.168544e-46
MsG0380012366.01	MsG0380013951.01	-0.816888	4.384285e-52	3.145090e-49
MsG0380012366.01	MsG0380013968.01	-0.817129	3.870103e-52	2.794607e-49
MsG0380012366.01	MsG0380014018.01	-0.818313	2.091854e-52	1.560451e-49
MsG0380012366.01	MsG0380014023.01	-0.808738	2.672412e-50	1.539933e-47
MsG0380012366.01	MsG0480021476.01	-0.818053	2.395118e-52	1.773869e-49
MsG0380012366.01	MsG0580024476.01	0.809666	1.690250e-50	9.984835e-48
MsG0380012366.01	MsG0580024570.01	-0.808146	3.574511e-50	2.028233e-47
MsG0380012366.01	MsG0580025052.01	-0.811035	8.561551e-51	5.243867e-48
MsG0380012366.01	MsG0580025155.01	0.802851	4.611758e-49	2.283008e-46
MsG0280009971.01	MsG0380012366.01	0.843891	1.059768e-58	1.682102e-55
MsG0280010145.01	MsG0380012366.01	-0.815257	1.014019e-51	6.956304e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012366.01.T01	MTR_3g023110	96.296	54	2	0	1	54	1	54	9.00e-35	115
MsG0380012366.01.T01	MTR_7g090630	60.000	65	24	1	1	63	1	65	5.91e-24	86.7
MsG0380012366.01.T01	MTR_7g090630	60.000	65	24	1	1	63	1	65	8.74e-24	86.7
MsG0380012366.01.T01	MTR_5g083690	65.116	43	15	0	8	50	11	53	3.30e-16	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012366.01.T01	AT2G41430	67.925	53	17	0	1	53	1	53	4.98e-24	87.4
MsG0380012366.01.T01	AT2G41430	67.925	53	17	0	1	53	1	53	1.19e-23	87.4
MsG0380012366.01.T01	AT2G41430	67.925	53	17	0	1	53	1	53	1.19e-23	87.4
MsG0380012366.01.T01	AT2G41430	67.925	53	17	0	1	53	1	53	1.19e-23	87.4
MsG0380012366.01.T01	AT2G41430	67.925	53	17	0	1	53	1	53	1.19e-23	87.4
MsG0380012366.01.T01	AT4G14270	56.604	53	23	0	2	54	3	55	2.32e-18	73.6
MsG0380012366.01.T01	AT4G14270	56.604	53	23	0	2	54	3	55	2.32e-18	73.6

Find 12 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGACATGGTTTCATAGCTAT+TGG	0.303499	3:+15535846	MsG0380012366.01.T01:CDS
GTTCTGATGGCATTGGTAAC+TGG	0.375492	3:+15535722	None:intergenic
GGTATGAAGAGAGGTGCATT+TGG	0.424438	3:-15535764	None:intergenic
ACAAAATCTTCTCCTTATGC+TGG	0.498388	3:-15535874	None:intergenic
CTATTGGTTGAGCCAGCATA+AGG	0.514718	3:+15535862	MsG0380012366.01.T01:CDS
ACCTGCTGCATTTCGTCAAG+TGG	0.534431	3:+15535784	MsG0380012366.01.T01:CDS
TTCACAAGCTCCCACCATTG+AGG	0.540449	3:-15535818	None:intergenic
TCCACTTGACGAAATGCAGC+AGG	0.554768	3:-15535785	None:intergenic
TGCTGCATTTCGTCAAGTGG+AGG	0.583030	3:+15535787	MsG0380012366.01.T01:CDS
AGCTTGTGAAATCTTCGACA+TGG	0.599712	3:+15535831	MsG0380012366.01.T01:CDS
CTGACAAGTTCTGATGGCAT+TGG	0.609871	3:+15535715	None:intergenic
AATGCAGCAGGTATGAAGAG+AGG	0.675950	3:-15535773	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAAAATCTTCTCCTTATGC+TGG	-	Chr3:15535877-15535896	None:intergenic	35.0%
	AGCTTGTGAAATCTTCGACA+TGG	+	Chr3:15535831-15535850	MsG0380012366.01.T01:CDS	40.0%
	CGACATGGTTTCATAGCTAT+TGG	+	Chr3:15535846-15535865	MsG0380012366.01.T01:CDS	40.0%
!	AGGATTTTTCACCTCAATGG+TGG	+	Chr3:15535807-15535826	MsG0380012366.01.T01:CDS	40.0%
!	GAAGATTTTGTCGAAAGTGG+CGG	+	Chr3:15535886-15535905	MsG0380012366.01.T01:CDS	40.0%
!	GGAGAAGATTTTGTCGAAAG+TGG	+	Chr3:15535883-15535902	MsG0380012366.01.T01:CDS	40.0%
!	GGATTTTTCACCTCAATGGT+GGG	+	Chr3:15535808-15535827	MsG0380012366.01.T01:CDS	40.0%
!	TGGAGGATTTTTCACCTCAA+TGG	+	Chr3:15535804-15535823	MsG0380012366.01.T01:CDS	40.0%
	AATGCAGCAGGTATGAAGAG+AGG	-	Chr3:15535776-15535795	None:intergenic	45.0%
!	CTATTGGTTGAGCCAGCATA+AGG	+	Chr3:15535862-15535881	MsG0380012366.01.T01:CDS	45.0%
!	GGTATGAAGAGAGGTGCATT+TGG	-	Chr3:15535767-15535786	None:intergenic	45.0%
	ACCTGCTGCATTTCGTCAAG+TGG	+	Chr3:15535784-15535803	MsG0380012366.01.T01:CDS	50.0%
	TCCACTTGACGAAATGCAGC+AGG	-	Chr3:15535788-15535807	None:intergenic	50.0%
	TGCTGCATTTCGTCAAGTGG+AGG	+	Chr3:15535787-15535806	MsG0380012366.01.T01:CDS	50.0%
	TTCACAAGCTCCCACCATTG+AGG	-	Chr3:15535821-15535840	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	15535728	15535937	15535728	ID=MsG0380012366.01;Name=MsG0380012366.01
Chr3	mRNA	15535728	15535937	15535728	ID=MsG0380012366.01.T01;Parent=MsG0380012366.01;Name=MsG0380012366.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|69
Chr3	exon	15535728	15535937	15535728	ID=MsG0380012366.01.T01:exon:943;Parent=MsG0380012366.01.T01
Chr3	CDS	15535728	15535937	15535728	ID=MsG0380012366.01.T01:cds;Parent=MsG0380012366.01.T01

Gene Sequence	>MsG0380012366.01.T01 ATGGCATTGGTAACTGGAAGAAGTTCAGCTCTGAATCCAAATGCACCTCTCTTCATACCTGCTGCATTTCGTCAAGTGGAGGATTTTTCACCTCAATGGTGGGAGCTTGTGAAATCTTCGACATGGTTTCATAGCTATTGGTTGAGCCAGCATAAGGAGAAGATTTTGTCGAAAGTGGCGGAAATGAAGCTACAAATGATGATGATATAG
Protein sequence	>MsG0380012366.01.T01 MALVTGRSSALNPNAPLFIPAAFRQVEDFSPQWWELVKSSTWFHSYWLSQHKEKILSKVAEMKLQMMMI*

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