AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024476.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024476.01.T01 MTR_5g011820 94.017 234 12 1 1 234 1 232 3.22e-159 440
MsG0580024476.01.T01 MTR_5g011800 90.909 231 19 2 4 234 1 229 1.03e-153 426
MsG0580024476.01.T01 MTR_4g102220 58.650 237 80 6 4 234 1 225 7.66e-80 239
MsG0580024476.01.T01 MTR_4g024370 50.633 237 106 5 4 234 1 232 7.11e-73 221
MsG0580024476.01.T01 MTR_4g024370 50.633 237 106 5 4 234 1 232 7.11e-73 221
MsG0580024476.01.T01 MTR_6g060390 30.603 232 132 9 8 233 4 212 9.60e-25 97.8
MsG0580024476.01.T01 MTR_6g060440 31.034 232 129 10 8 233 4 210 6.10e-24 95.9
MsG0580024476.01.T01 MTR_7g117380 29.694 229 148 6 8 233 3 221 1.35e-20 87.0
MsG0580024476.01.T01 MTR_1g076800 27.160 243 127 8 7 233 3 211 5.41e-19 82.8
MsG0580024476.01.T01 MTR_4g023950 29.825 228 134 8 8 233 4 207 5.77e-16 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024476.01.T01 AT2G45360 53.017 232 91 5 4 234 1 215 3.54e-73 222
MsG0580024476.01.T01 AT3G60780 51.282 234 95 6 4 234 1 218 4.27e-70 214
MsG0580024476.01.T01 AT1G62840 47.083 240 102 8 4 234 1 224 1.15e-54 175
MsG0580024476.01.T01 AT1G12320 43.103 232 103 7 4 230 1 208 7.33e-45 149

Find 65 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTTCTAAGATGGATTC+TGG 0.260989 5:-5418140 MsG0580024476.01.T01:CDS
TTTAGTAGGAATAGATTTCT+TGG 0.332754 5:-5418255 MsG0580024476.01.T01:CDS
TATGAATTAGTTGCTCTCTC+TGG 0.350428 5:+5418578 None:intergenic
AATCACCTTGTCAGTGAATC+TGG 0.357557 5:-5418521 MsG0580024476.01.T01:CDS
TGTTCGGTCGGTGTTTCTTC+CGG 0.371763 5:-5418075 MsG0580024476.01.T01:CDS
AGATGGATTCTGGTTATATA+TGG 0.373750 5:-5418130 MsG0580024476.01.T01:CDS
TGGTATGTCACCGGAAGTTT+TGG 0.381944 5:-5418306 MsG0580024476.01.T01:CDS
TGAAAGACATGAAACTTGTT+TGG 0.393646 5:-5418604 MsG0580024476.01.T01:CDS
ATTTCTTGGTTGTTGATTCT+CGG 0.396616 5:-5418241 MsG0580024476.01.T01:CDS
TCTGATCTTGACCGTTCATC+TGG 0.402447 5:+5418353 None:intergenic
CACGTGTTATTCATAATAGA+TGG 0.406729 5:-5417974 MsG0580024476.01.T01:CDS
GGTGTTTCTTCCGGTGGGGA+AGG 0.435607 5:-5418066 MsG0580024476.01.T01:CDS
ATGAATTAGTTGCTCTCTCT+GGG 0.444178 5:+5418579 None:intergenic
CGGTCGGTGTTTCTTCCGGT+GGG 0.457893 5:-5418071 MsG0580024476.01.T01:CDS
GAAGTGATGGATGGTTTAGT+AGG 0.459582 5:-5418269 MsG0580024476.01.T01:CDS
CATTTATCAGCTGCCGGTGG+TGG 0.462107 5:-5418029 MsG0580024476.01.T01:CDS
GGCGGAGTTTGTTTCAGCTA+TGG 0.469299 5:-5418495 MsG0580024476.01.T01:CDS
ATGGCGAAGCGTAGTGGCGC+GGG 0.470110 5:-5418111 MsG0580024476.01.T01:CDS
AAGCATGTTGATCAACGGTC+CGG 0.476390 5:-5417948 MsG0580024476.01.T01:CDS
AGCATGTTGATCAACGGTCC+GGG 0.480427 5:-5417947 MsG0580024476.01.T01:CDS
GTGTTTCTTCCGGTGGGGAA+GGG 0.481603 5:-5418065 MsG0580024476.01.T01:CDS
AGATTCACTGACAAGGTGAT+TGG 0.483078 5:+5418523 None:intergenic
GTCACGACGGCTTGTTCGGT+CGG 0.491936 5:-5418087 MsG0580024476.01.T01:CDS
GGTTCTCCTACCAAAACTTC+CGG 0.492682 5:+5418296 None:intergenic
TTTGTTTCAGCTATGGCGGC+AGG 0.510446 5:-5418488 MsG0580024476.01.T01:CDS
TTAGCAAAGTTGAGTTGTAA+AGG 0.512051 5:-5418191 MsG0580024476.01.T01:CDS
CACAGAGTTCTCACCACCAC+CGG 0.513456 5:+5418016 None:intergenic
ACCTGTGTGACGTGATGCTA+TGG 0.522581 5:+5418397 None:intergenic
TTCACTGACAAGGTGATTGG+TGG 0.530233 5:+5418526 None:intergenic
AAACCATCCATCACTTCCTC+CGG 0.536217 5:+5418275 None:intergenic
TCTAATAAAGTGAACATCCC+CGG 0.541948 5:+5417929 None:intergenic
AGGAGAACCGGAGGAAGTGA+TGG 0.549491 5:-5418282 MsG0580024476.01.T01:CDS
GGTGGGGAAGGGAATTCATA+TGG 0.553993 5:-5418054 MsG0580024476.01.T01:CDS
CGTTGGAATAACTCCGCCAT+GGG 0.559266 5:+5418433 None:intergenic
GGAGTTATTCCAACGAGTGT+TGG 0.565148 5:-5418425 MsG0580024476.01.T01:CDS
TCGGTCGGTGTTTCTTCCGG+TGG 0.566963 5:-5418072 MsG0580024476.01.T01:CDS
TGGCGAAGCGTAGTGGCGCG+GGG 0.569786 5:-5418110 MsG0580024476.01.T01:CDS
TTTCAGCTATGGCGGCAGGA+TGG 0.575184 5:-5418484 MsG0580024476.01.T01:CDS
ATGGCGCATTTATCAGCTGC+CGG 0.576415 5:-5418035 MsG0580024476.01.T01:CDS
CTCAGCTGATAGTAGAAACA+TGG 0.579071 5:-5418457 MsG0580024476.01.T01:CDS
TTATATATGGCGAAGCGTAG+TGG 0.584131 5:-5418117 MsG0580024476.01.T01:CDS
TGGTGAGAACTCTGTGGTGG+CGG 0.585821 5:-5418009 MsG0580024476.01.T01:CDS
ATATGAATTCCCTTCCCCAC+CGG 0.588515 5:+5418056 None:intergenic
ATGGATAAACCAACACTCGT+TGG 0.590421 5:+5418416 None:intergenic
GGGAGAAGCTGGTATGTCAC+CGG 0.594972 5:-5418315 MsG0580024476.01.T01:CDS
TCGTTGGAATAACTCCGCCA+TGG 0.605659 5:+5418432 None:intergenic
GGTCGGTGTTTCTTCCGGTG+GGG 0.609408 5:-5418070 MsG0580024476.01.T01:CDS
GAACCGGAGGAAGTGATGGA+TGG 0.618455 5:-5418278 MsG0580024476.01.T01:CDS
GGAGTTTGTTTCAGCTATGG+CGG 0.620173 5:-5418492 MsG0580024476.01.T01:CDS
TGTGTATAGTTCCAGATGAA+CGG 0.623096 5:-5418364 MsG0580024476.01.T01:CDS
ATAGTAGAAACATGGTCCCA+TGG 0.627081 5:-5418449 MsG0580024476.01.T01:CDS
GCATGTTGATCAACGGTCCG+GGG 0.627112 5:-5417946 MsG0580024476.01.T01:CDS
GGCGAAGCGTAGTGGCGCGG+GGG 0.628973 5:-5418109 MsG0580024476.01.T01:CDS
TATGGCGAAGCGTAGTGGCG+CGG 0.632704 5:-5418112 MsG0580024476.01.T01:CDS
TGGTGGTGAGAACTCTGTGG+TGG 0.632865 5:-5418012 MsG0580024476.01.T01:CDS
CCACACCAGATTCACTGACA+AGG 0.641093 5:+5418516 None:intergenic
GGATTAAGCATGTTGATCAA+CGG 0.643256 5:-5417953 MsG0580024476.01.T01:CDS
TCCATAGCATCACGTCACAC+AGG 0.650589 5:-5418398 MsG0580024476.01.T01:CDS
ATAGCATCACGTCACACAGG+TGG 0.657777 5:-5418395 MsG0580024476.01.T01:CDS
CGGTGGTGGTGAGAACTCTG+TGG 0.659832 5:-5418015 MsG0580024476.01.T01:CDS
CCTTGTCAGTGAATCTGGTG+TGG 0.666939 5:-5418516 MsG0580024476.01.T01:CDS
GCGCATTTATCAGCTGCCGG+TGG 0.672929 5:-5418032 MsG0580024476.01.T01:CDS
TGTCAGTGAATCTGGTGTGG+CGG 0.676538 5:-5418513 MsG0580024476.01.T01:CDS
TGAATTAGTTGCTCTCTCTG+GGG 0.679617 5:+5418580 None:intergenic
GTAGAAACATGGTCCCATGG+CGG 0.759198 5:-5418446 MsG0580024476.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGCTAATTTTATTTCTAAGA+TGG - Chr5:5418381-5418400 MsG0580024476.01.T01:CDS 20.0%
! TTTAGTAGGAATAGATTTCT+TGG - Chr5:5418273-5418292 MsG0580024476.01.T01:CDS 25.0%
!!! TTTATTTCTAAGATGGATTC+TGG - Chr5:5418388-5418407 MsG0580024476.01.T01:CDS 25.0%
ATCAGAGTATGCAAAAAACA+TGG - Chr5:5418192-5418211 MsG0580024476.01.T01:CDS 30.0%
TCAGAGTATGCAAAAAACAT+GGG - Chr5:5418193-5418212 MsG0580024476.01.T01:CDS 30.0%
! AGATGGATTCTGGTTATATA+TGG - Chr5:5418398-5418417 MsG0580024476.01.T01:CDS 30.0%
! CACGTGTTATTCATAATAGA+TGG - Chr5:5418554-5418573 MsG0580024476.01.T01:CDS 30.0%
! TGAAAGACATGAAACTTGTT+TGG - Chr5:5417924-5417943 MsG0580024476.01.T01:CDS 30.0%
! TTAGCAAAGTTGAGTTGTAA+AGG - Chr5:5418337-5418356 MsG0580024476.01.T01:CDS 30.0%
!! ATTTCTTGGTTGTTGATTCT+CGG - Chr5:5418287-5418306 MsG0580024476.01.T01:CDS 30.0%
!!! AAGATTTTACAAGAGTGTTG+AGG - Chr5:5418314-5418333 MsG0580024476.01.T01:CDS 30.0%
ATGAATTAGTTGCTCTCTCT+GGG + Chr5:5417952-5417971 None:intergenic 35.0%
GGATTAAGCATGTTGATCAA+CGG - Chr5:5418575-5418594 MsG0580024476.01.T01:CDS 35.0%
TATGAATTAGTTGCTCTCTC+TGG + Chr5:5417953-5417972 None:intergenic 35.0%
TCTAATAAAGTGAACATCCC+CGG + Chr5:5418602-5418621 None:intergenic 35.0%
TGTGTATAGTTCCAGATGAA+CGG - Chr5:5418164-5418183 MsG0580024476.01.T01:CDS 35.0%
AATCACCTTGTCAGTGAATC+TGG - Chr5:5418007-5418026 MsG0580024476.01.T01:CDS 40.0%
AGATTCACTGACAAGGTGAT+TGG + Chr5:5418008-5418027 None:intergenic 40.0%
ATAGTAGAAACATGGTCCCA+TGG - Chr5:5418079-5418098 MsG0580024476.01.T01:CDS 40.0%
ATGGATAAACCAACACTCGT+TGG + Chr5:5418115-5418134 None:intergenic 40.0%
CTCAGCTGATAGTAGAAACA+TGG - Chr5:5418071-5418090 MsG0580024476.01.T01:CDS 40.0%
TGAATTAGTTGCTCTCTCTG+GGG + Chr5:5417951-5417970 None:intergenic 40.0%
TTATATATGGCGAAGCGTAG+TGG - Chr5:5418411-5418430 MsG0580024476.01.T01:CDS 40.0%
!! GAAGTGATGGATGGTTTAGT+AGG - Chr5:5418259-5418278 MsG0580024476.01.T01:CDS 40.0%
AAACCATCCATCACTTCCTC+CGG + Chr5:5418256-5418275 None:intergenic 45.0%
AAGCATGTTGATCAACGGTC+CGG - Chr5:5418580-5418599 MsG0580024476.01.T01:CDS 45.0%
ATATGAATTCCCTTCCCCAC+CGG + Chr5:5418475-5418494 None:intergenic 45.0%
GCAAAAAACATGGGAGAAGC+TGG - Chr5:5418202-5418221 MsG0580024476.01.T01:CDS 45.0%
GGAGTTATTCCAACGAGTGT+TGG - Chr5:5418103-5418122 MsG0580024476.01.T01:CDS 45.0%
GGTTCTCCTACCAAAACTTC+CGG + Chr5:5418235-5418254 None:intergenic 45.0%
TCTGATCTTGACCGTTCATC+TGG + Chr5:5418178-5418197 None:intergenic 45.0%
TTCACTGACAAGGTGATTGG+TGG + Chr5:5418005-5418024 None:intergenic 45.0%
! GGAGTTTGTTTCAGCTATGG+CGG - Chr5:5418036-5418055 MsG0580024476.01.T01:CDS 45.0%
! TGGTATGTCACCGGAAGTTT+TGG - Chr5:5418222-5418241 MsG0580024476.01.T01:CDS 45.0%
!! ATGTCACCGGAAGTTTTGGT+AGG - Chr5:5418226-5418245 MsG0580024476.01.T01:CDS 45.0%
!! GGAAGTTTTGGTAGGAGAAC+CGG - Chr5:5418234-5418253 MsG0580024476.01.T01:CDS 45.0%
ACCTGTGTGACGTGATGCTA+TGG + Chr5:5418134-5418153 None:intergenic 50.0%
AGCATGTTGATCAACGGTCC+GGG - Chr5:5418581-5418600 MsG0580024476.01.T01:CDS 50.0%
ATAGCATCACGTCACACAGG+TGG - Chr5:5418133-5418152 MsG0580024476.01.T01:CDS 50.0%
ATGGCGCATTTATCAGCTGC+CGG - Chr5:5418493-5418512 MsG0580024476.01.T01:CDS 50.0%
CCACACCAGATTCACTGACA+AGG + Chr5:5418015-5418034 None:intergenic 50.0%
CCTTGTCAGTGAATCTGGTG+TGG - Chr5:5418012-5418031 MsG0580024476.01.T01:CDS 50.0%
CGTTGGAATAACTCCGCCAT+GGG + Chr5:5418098-5418117 None:intergenic 50.0%
GGTGGGGAAGGGAATTCATA+TGG - Chr5:5418474-5418493 MsG0580024476.01.T01:CDS 50.0%
GTAGAAACATGGTCCCATGG+CGG - Chr5:5418082-5418101 MsG0580024476.01.T01:CDS 50.0%
TCCATAGCATCACGTCACAC+AGG - Chr5:5418130-5418149 MsG0580024476.01.T01:CDS 50.0%
TCGTTGGAATAACTCCGCCA+TGG + Chr5:5418099-5418118 None:intergenic 50.0%
TGTCAGTGAATCTGGTGTGG+CGG - Chr5:5418015-5418034 MsG0580024476.01.T01:CDS 50.0%
TTTGTTTCAGCTATGGCGGC+AGG - Chr5:5418040-5418059 MsG0580024476.01.T01:CDS 50.0%
! GGCGGAGTTTGTTTCAGCTA+TGG - Chr5:5418033-5418052 MsG0580024476.01.T01:CDS 50.0%
! TGTTCGGTCGGTGTTTCTTC+CGG - Chr5:5418453-5418472 MsG0580024476.01.T01:CDS 50.0%
!! AGTTTTGGTAGGAGAACCGG+AGG - Chr5:5418237-5418256 MsG0580024476.01.T01:CDS 50.0%
AGGAGAACCGGAGGAAGTGA+TGG - Chr5:5418246-5418265 MsG0580024476.01.T01:CDS 55.0%
CACAGAGTTCTCACCACCAC+CGG + Chr5:5418515-5418534 None:intergenic 55.0%
CATTTATCAGCTGCCGGTGG+TGG - Chr5:5418499-5418518 MsG0580024476.01.T01:CDS 55.0%
GCATGTTGATCAACGGTCCG+GGG - Chr5:5418582-5418601 MsG0580024476.01.T01:CDS 55.0%
GGGAGAAGCTGGTATGTCAC+CGG - Chr5:5418213-5418232 MsG0580024476.01.T01:CDS 55.0%
GTGTTTCTTCCGGTGGGGAA+GGG - Chr5:5418463-5418482 MsG0580024476.01.T01:CDS 55.0%
TGGTGAGAACTCTGTGGTGG+CGG - Chr5:5418519-5418538 MsG0580024476.01.T01:CDS 55.0%
TTTCAGCTATGGCGGCAGGA+TGG - Chr5:5418044-5418063 MsG0580024476.01.T01:CDS 55.0%
! GAACCGGAGGAAGTGATGGA+TGG - Chr5:5418250-5418269 MsG0580024476.01.T01:CDS 55.0%
! TGGTGGTGAGAACTCTGTGG+TGG - Chr5:5418516-5418535 MsG0580024476.01.T01:CDS 55.0%
GCGCATTTATCAGCTGCCGG+TGG - Chr5:5418496-5418515 MsG0580024476.01.T01:CDS 60.0%
GGTGTTTCTTCCGGTGGGGA+AGG - Chr5:5418462-5418481 MsG0580024476.01.T01:CDS 60.0%
TATGGCGAAGCGTAGTGGCG+CGG - Chr5:5418416-5418435 MsG0580024476.01.T01:CDS 60.0%
! CGGTCGGTGTTTCTTCCGGT+GGG - Chr5:5418457-5418476 MsG0580024476.01.T01:CDS 60.0%
! CGGTGGTGGTGAGAACTCTG+TGG - Chr5:5418513-5418532 MsG0580024476.01.T01:CDS 60.0%
! GGTCGGTGTTTCTTCCGGTG+GGG - Chr5:5418458-5418477 MsG0580024476.01.T01:CDS 60.0%
! GTCACGACGGCTTGTTCGGT+CGG - Chr5:5418441-5418460 MsG0580024476.01.T01:CDS 60.0%
! TCGGTCGGTGTTTCTTCCGG+TGG - Chr5:5418456-5418475 MsG0580024476.01.T01:CDS 60.0%
ATGGCGAAGCGTAGTGGCGC+GGG - Chr5:5418417-5418436 MsG0580024476.01.T01:CDS 65.0%
! GGGGGTCACGACGGCTTGTT+CGG - Chr5:5418437-5418456 MsG0580024476.01.T01:CDS 65.0%
TAGTGGCGCGGGGGTCACGA+CGG - Chr5:5418428-5418447 MsG0580024476.01.T01:CDS 70.0%
TGGCGAAGCGTAGTGGCGCG+GGG - Chr5:5418418-5418437 MsG0580024476.01.T01:CDS 70.0%
GGCGAAGCGTAGTGGCGCGG+GGG - Chr5:5418419-5418438 MsG0580024476.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr5 gene 5417923 5418627 5417923 ID=MsG0580024476.01;Name=MsG0580024476.01
Chr5 mRNA 5417923 5418627 5417923 ID=MsG0580024476.01.T01;Parent=MsG0580024476.01;Name=MsG0580024476.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|234
Chr5 exon 5417923 5418627 5417923 ID=MsG0580024476.01.T01:exon:25662;Parent=MsG0580024476.01.T01
Chr5 CDS 5417923 5418627 5417923 ID=MsG0580024476.01.T01:cds;Parent=MsG0580024476.01.T01
Gene Sequence

>MsG0580024476.01.T01

ATGAAAGACATGAAACTTGTTTGGTCCCCAGAGAGAGCAACTAATTCATACATAGACACAGTTCAAGCACTAGCAGTAACCACCAATCACCTTGTCAGTGAATCTGGTGTGGCGGAGTTTGTTTCAGCTATGGCGGCAGGATGGAATGCTCAGCTGATAGTAGAAACATGGTCCCATGGCGGAGTTATTCCAACGAGTGTTGGTTTATCCATAGCATCACGTCACACAGGTGGAAGACATGTGTGTATAGTTCCAGATGAACGGTCAAGATCAGAGTATGCAAAAAACATGGGAGAAGCTGGTATGTCACCGGAAGTTTTGGTAGGAGAACCGGAGGAAGTGATGGATGGTTTAGTAGGAATAGATTTCTTGGTTGTTGATTCTCGGAGAAAAGATTTTACAAGAGTGTTGAGGTTAGCAAAGTTGAGTTGTAAAGGTGCTGTGTTGATATGTAAGAATGCTAATTTTATTTCTAAGATGGATTCTGGTTATATATGGCGAAGCGTAGTGGCGCGGGGGTCACGACGGCTTGTTCGGTCGGTGTTTCTTCCGGTGGGGAAGGGAATTCATATGGCGCATTTATCAGCTGCCGGTGGTGGTGAGAACTCTGTGGTGGCGGTGAAGCATAAAGCACGTGTTATTCATAATAGATGGATTAAGCATGTTGATCAACGGTCCGGGGATGTTCACTTTATTAGAAAATGA

Protein sequence

>MsG0580024476.01.T01

MKDMKLVWSPERATNSYIDTVQALAVTTNHLVSESGVAEFVSAMAAGWNAQLIVETWSHGGVIPTSVGLSIASRHTGGRHVCIVPDERSRSEYAKNMGEAGMSPEVLVGEPEEVMDGLVGIDFLVVDSRRKDFTRVLRLAKLSCKGAVLICKNANFISKMDSGYIWRSVVARGSRRLVRSVFLPVGKGIHMAHLSAAGGGENSVVAVKHKARVIHNRWIKHVDQRSGDVHFIRK*