AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016129.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016129.01.T01 MTR_3g083920 62.882 229 23 3 1 167 59 287 2.95e-86 258
MsG0380016129.01.T01 MTR_3g083910 60.262 229 29 3 1 167 59 287 3.33e-83 250
MsG0380016129.01.T01 MTR_5g007713 58.079 229 34 3 1 167 59 287 3.51e-81 245
MsG0380016129.01.T01 MTR_1g097935 58.079 229 34 3 1 167 59 287 3.79e-81 245
MsG0380016129.01.T01 MTR_1g097910 58.079 229 34 3 1 167 59 287 4.41e-81 245
MsG0380016129.01.T01 MTR_7g016800 58.079 229 34 3 1 167 59 287 4.82e-81 245
MsG0380016129.01.T01 MTR_5g007717 58.079 229 34 3 1 167 59 287 5.31e-81 245
MsG0380016129.01.T01 MTR_1g098140 58.079 229 34 3 1 167 59 287 5.67e-81 244
MsG0380016129.01.T01 MTR_7g016720 58.079 229 34 3 1 167 59 287 6.39e-81 244
MsG0380016129.01.T01 MTR_7g016820 58.079 229 34 3 1 167 59 287 7.12e-81 244
MsG0380016129.01.T01 MTR_7g016780 58.079 229 34 3 1 167 59 287 8.75e-81 244
MsG0380016129.01.T01 MTR_7g016700 57.642 229 35 3 1 167 59 287 9.54e-81 244
MsG0380016129.01.T01 MTR_5g007730 57.642 229 35 3 1 167 59 287 1.64e-80 243
MsG0380016129.01.T01 MTR_5g007760 57.642 229 35 3 1 167 59 287 1.29e-79 241
MsG0380016129.01.T01 MTR_3g086260 54.585 229 42 3 1 167 59 287 8.11e-73 224
MsG0380016129.01.T01 MTR_2g058470 53.712 229 44 3 1 167 59 287 5.69e-72 222
MsG0380016129.01.T01 MTR_7g084300 52.609 230 47 3 1 168 59 288 8.41e-72 221
MsG0380016129.01.T01 MTR_5g007740 47.768 224 55 3 6 167 1 224 2.83e-58 185
MsG0380016129.01.T01 MTR_7g113405 52.239 201 34 3 29 167 1 201 1.03e-56 180
MsG0380016129.01.T01 MTR_3g088675 44.541 229 65 3 1 167 59 287 3.68e-56 181
MsG0380016129.01.T01 MTR_5g007770 48.259 201 42 3 12 150 1 201 1.05e-52 169
MsG0380016129.01.T01 MTR_5g007723 45.771 201 73 5 2 167 97 296 1.29e-49 164
MsG0380016129.01.T01 MTR_4g075560 29.565 230 94 4 1 165 60 286 5.45e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016129.01.T01 AT5G13930 53.478 230 44 4 1 167 64 293 3.09e-69 215
MsG0380016129.01.T01 AT4G34850 30.396 227 90 5 1 162 61 284 5.97e-25 99.4
MsG0380016129.01.T01 AT4G34850 30.374 214 81 5 14 162 1 211 5.73e-21 87.8

Find 58 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGAACTTAAGAAGAATT+TGG 0.237520 3:+79417022 None:intergenic
AACAAAGGCTTCTCAACTTC+TGG 0.283514 3:+79416202 None:intergenic
CATCATAGCCCTTCACTATC+TGG 0.302856 3:+79416149 None:intergenic
TCTTGTGGGGCAGGCATGTT+TGG 0.341798 3:-79416265 MsG0380016129.01.T01:intron
GCTTCGTTTGGCTAAAGATT+TGG 0.342769 3:-79416377 MsG0380016129.01.T01:CDS
GGCTTTCAGCTCTCTGTGCC+TGG 0.347822 3:+79416953 None:intergenic
CATCTCCTCAAGGATGTTCC+TGG 0.370213 3:-79416097 MsG0380016129.01.T01:CDS
AAGCCTTTGTTTGAATGGTT+TGG 0.377148 3:-79416190 MsG0380016129.01.T01:CDS
ATTTGGTTGGTAAGAGAAGA+AGG 0.388826 3:+79417039 None:intergenic
CCTGCCACTGTCTTTGCAAT+AGG 0.391107 3:-79416932 MsG0380016129.01.T01:five_prime_UTR
ATTGGATGCAAGACAGGATA+TGG 0.394068 3:-79416615 MsG0380016129.01.T01:CDS
TTGAGAAGCCTTTGTTTGAA+TGG 0.402664 3:-79416195 MsG0380016129.01.T01:CDS
CGTTATATGATGTATCAAAA+AGG 0.407162 3:-79416421 MsG0380016129.01.T01:CDS
TGAGTACATGGCACCTTCAT+TGG 0.415295 3:-79416633 MsG0380016129.01.T01:CDS
AACAAAGGCTATCAAGGAAT+GGG 0.424210 3:-79416555 MsG0380016129.01.T01:CDS
TGGTGGCACGGTGCTTCGTT+TGG 0.426992 3:-79416389 MsG0380016129.01.T01:CDS
ATGTTCTTTGAGACGAGGCC+AGG 0.453712 3:+79416079 None:intergenic
ACCTTCATTGGATGCAAGAC+AGG 0.464180 3:-79416621 MsG0380016129.01.T01:CDS
GGCTGCAACAAAGGCTATCA+AGG 0.472973 3:-79416561 MsG0380016129.01.T01:CDS
ATCATATAACGCTTCACAGA+CGG 0.478105 3:+79416433 None:intergenic
CAGATAGTGAAGGGCTATGA+TGG 0.484821 3:-79416148 MsG0380016129.01.T01:CDS
ATTTATATTCTTCATTGTGA+TGG 0.492915 3:-79416995 MsG0380016129.01.T01:five_prime_UTR
CCTATTGCAAAGACAGTGGC+AGG 0.496706 3:+79416932 None:intergenic
GGTGCAGCAGCTGTGATTGT+TGG 0.502726 3:-79416238 MsG0380016129.01.T01:CDS
AGGAAAAGAGGCTGCAACAA+AGG 0.503571 3:-79416570 MsG0380016129.01.T01:CDS
TCATATAACGCTTCACAGAC+GGG 0.503757 3:+79416434 None:intergenic
CAACAAAGGCTATCAAGGAA+TGG 0.506508 3:-79416556 MsG0380016129.01.T01:CDS
GAACTTAAGAAGAATTTGGT+TGG 0.506904 3:+79417026 None:intergenic
GGGGCAGGCATGTTTGGAGA+TGG 0.507939 3:-79416259 MsG0380016129.01.T01:intron
TCCTGTCTTGCATCCAATGA+AGG 0.510738 3:+79416620 None:intergenic
GATGGTCACCTTCGAGAAGC+AGG 0.523036 3:-79416130 MsG0380016129.01.T01:CDS
CAGGAACATCCTTGAGGAGA+TGG 0.526587 3:+79416098 None:intergenic
TGTGTTCACTGTTTGTGATA+CGG 0.533500 3:+79416856 None:intergenic
TGTGATACGGAAGTAAAGTC+AGG 0.543563 3:+79416869 None:intergenic
GGCTTCTCAACTTCTGGCAA+CGG 0.544110 3:+79416208 None:intergenic
TATATATCTTACACATGCGC+TGG 0.547544 3:+79416811 None:intergenic
TCCTAGTTTATGTGAGTACA+TGG 0.548879 3:-79416645 MsG0380016129.01.T01:CDS
AGTACCAAAGCTAGGAAAAG+AGG 0.551914 3:-79416582 MsG0380016129.01.T01:CDS
TTTGACTGAAGAGATTCTGA+AGG 0.562158 3:-79416672 MsG0380016129.01.T01:CDS
AAGACAGGATATGGTGGTTG+TGG 0.581715 3:-79416606 MsG0380016129.01.T01:CDS
GACAGTGAATGAGATACGCC+AGG 0.598521 3:-79416971 MsG0380016129.01.T01:five_prime_UTR
TTGACTGAAGAGATTCTGAA+GGG 0.603023 3:-79416671 MsG0380016129.01.T01:CDS
TTGTGTGAGAAAATGTTGTG+TGG 0.603818 3:+79417086 None:intergenic
TCTCAATGTTCTTTGAGACG+AGG 0.604924 3:+79416074 None:intergenic
CAATCGTTCCAGATAGTGAA+GGG 0.608661 3:-79416157 MsG0380016129.01.T01:CDS
ATGTCAGTCCTGCTTCTCGA+AGG 0.612769 3:+79416122 None:intergenic
ACTGACATTCCATCTCCTCA+AGG 0.613763 3:-79416107 MsG0380016129.01.T01:CDS
GTTGTGGAAGTACCAAAGCT+AGG 0.617435 3:-79416590 MsG0380016129.01.T01:CDS
ACAATCGTTCCAGATAGTGA+AGG 0.620808 3:-79416158 MsG0380016129.01.T01:CDS
GCCATGTACTCACATAAACT+AGG 0.622323 3:+79416644 None:intergenic
TGACTGAAGAGATTCTGAAG+GGG 0.645559 3:-79416670 MsG0380016129.01.T01:CDS
ACATTGAGAAGCTCTTGTTG+AGG 0.652597 3:-79416060 MsG0380016129.01.T01:CDS
AGTCCAAACCATTCAAACAA+AGG 0.662757 3:+79416187 None:intergenic
ACAAAGGCTATCAAGGAATG+GGG 0.667647 3:-79416554 MsG0380016129.01.T01:intron
CGAGGCCAGGAACATCCTTG+AGG 0.671207 3:+79416092 None:intergenic
GATTTGGCTGAAAACAACAA+AGG 0.694413 3:-79416361 MsG0380016129.01.T01:intron
AGTGCCTATTGCAAAGACAG+TGG 0.727850 3:+79416928 None:intergenic
GGATGCAAGACAGGATATGG+TGG 0.728808 3:-79416612 MsG0380016129.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CACATAAAGATATATAATAT+AGG + Chr3:79416361-79416380 None:intergenic 15.0%
!! AAATGAACTTAAGAAGAATT+TGG + Chr3:79416121-79416140 None:intergenic 20.0%
!! ATTTATATTCTTCATTGTGA+TGG - Chr3:79416145-79416164 MsG0380016129.01.T01:CDS 20.0%
! CGTTATATGATGTATCAAAA+AGG - Chr3:79416719-79416738 MsG0380016129.01.T01:intron 25.0%
GAACTTAAGAAGAATTTGGT+TGG + Chr3:79416117-79416136 None:intergenic 30.0%
! TTATGCTGTGTATGATTTTC+AGG - Chr3:79416412-79416431 MsG0380016129.01.T01:CDS 30.0%
!! TATCAAAAAGGTTGTTTTGC+TGG - Chr3:79416731-79416750 MsG0380016129.01.T01:intron 30.0%
AACAAAGGCTATCAAGGAAT+GGG - Chr3:79416585-79416604 MsG0380016129.01.T01:CDS 35.0%
ACTATCAGATGAGTATCACT+AGG + Chr3:79416856-79416875 None:intergenic 35.0%
AGTCCAAACCATTCAAACAA+AGG + Chr3:79416956-79416975 None:intergenic 35.0%
ATCATATAACGCTTCACAGA+CGG + Chr3:79416710-79416729 None:intergenic 35.0%
GATTTGGCTGAAAACAACAA+AGG - Chr3:79416779-79416798 MsG0380016129.01.T01:intron 35.0%
TATATATCTTACACATGCGC+TGG + Chr3:79416332-79416351 None:intergenic 35.0%
TATCAGCTCACAAAGCTATT+AGG - Chr3:79416677-79416696 MsG0380016129.01.T01:CDS 35.0%
TCCTAGTTTATGTGAGTACA+TGG - Chr3:79416495-79416514 MsG0380016129.01.T01:intron 35.0%
TGTGTTCACTGTTTGTGATA+CGG + Chr3:79416287-79416306 None:intergenic 35.0%
TTGACTGAAGAGATTCTGAA+GGG - Chr3:79416469-79416488 MsG0380016129.01.T01:intron 35.0%
TTGTGTGAGAAAATGTTGTG+TGG + Chr3:79416057-79416076 None:intergenic 35.0%
TTTGACTGAAGAGATTCTGA+AGG - Chr3:79416468-79416487 MsG0380016129.01.T01:intron 35.0%
! AAGCCTTTGTTTGAATGGTT+TGG - Chr3:79416950-79416969 MsG0380016129.01.T01:five_prime_UTR 35.0%
! ATACTCATCTGATAGTCTTG+TGG - Chr3:79416860-79416879 MsG0380016129.01.T01:five_prime_UTR 35.0%
! TCTAATTTTTGCACCACAAG+TGG - Chr3:79416632-79416651 MsG0380016129.01.T01:CDS 35.0%
! TTGAGAAGCCTTTGTTTGAA+TGG - Chr3:79416945-79416964 MsG0380016129.01.T01:five_prime_UTR 35.0%
!! ATTTGGTTGGTAAGAGAAGA+AGG + Chr3:79416104-79416123 None:intergenic 35.0%
!! TACTCATCTGATAGTCTTGT+GGG - Chr3:79416861-79416880 MsG0380016129.01.T01:five_prime_UTR 35.0%
AACAAAGGCTTCTCAACTTC+TGG + Chr3:79416941-79416960 None:intergenic 40.0%
ACAAAGGCTATCAAGGAATG+GGG - Chr3:79416586-79416605 MsG0380016129.01.T01:CDS 40.0%
ACAATCGTTCCAGATAGTGA+AGG - Chr3:79416982-79417001 MsG0380016129.01.T01:five_prime_UTR 40.0%
ACATTGAGAAGCTCTTGTTG+AGG - Chr3:79417080-79417099 MsG0380016129.01.T01:five_prime_UTR 40.0%
AGTACCAAAGCTAGGAAAAG+AGG - Chr3:79416558-79416577 MsG0380016129.01.T01:CDS 40.0%
ATTGGATGCAAGACAGGATA+TGG - Chr3:79416525-79416544 MsG0380016129.01.T01:intron 40.0%
CAACAAAGGCTATCAAGGAA+TGG - Chr3:79416584-79416603 MsG0380016129.01.T01:CDS 40.0%
CAATCGTTCCAGATAGTGAA+GGG - Chr3:79416983-79417002 MsG0380016129.01.T01:five_prime_UTR 40.0%
GCCATGTACTCACATAAACT+AGG + Chr3:79416499-79416518 None:intergenic 40.0%
GCTTCGTTTGGCTAAAGATT+TGG - Chr3:79416763-79416782 MsG0380016129.01.T01:intron 40.0%
TCATATAACGCTTCACAGAC+GGG + Chr3:79416709-79416728 None:intergenic 40.0%
TGTGATACGGAAGTAAAGTC+AGG + Chr3:79416274-79416293 None:intergenic 40.0%
! CTCTGATCAACACAGTTTTG+AGG + Chr3:79416244-79416263 None:intergenic 40.0%
! TGACTGAAGAGATTCTGAAG+GGG - Chr3:79416470-79416489 MsG0380016129.01.T01:intron 40.0%
!! ACTCATCTGATAGTCTTGTG+GGG - Chr3:79416862-79416881 MsG0380016129.01.T01:five_prime_UTR 40.0%
!! CAAAAAGGTTGTTTTGCTGG+TGG - Chr3:79416734-79416753 MsG0380016129.01.T01:intron 40.0%
!! TCTCAATGTTCTTTGAGACG+AGG + Chr3:79417069-79417088 None:intergenic 40.0%
!!! ACAACAAAGGTGCTGTGTTT+TGG - Chr3:79416792-79416811 MsG0380016129.01.T01:intron 40.0%
!!! CTGTGTTTTGGTGGTTTGTT+CGG - Chr3:79416804-79416823 MsG0380016129.01.T01:intron 40.0%
AAGACAGGATATGGTGGTTG+TGG - Chr3:79416534-79416553 MsG0380016129.01.T01:intron 45.0%
ACCTTCATTGGATGCAAGAC+AGG - Chr3:79416519-79416538 MsG0380016129.01.T01:intron 45.0%
ACTGACATTCCATCTCCTCA+AGG - Chr3:79417033-79417052 MsG0380016129.01.T01:five_prime_UTR 45.0%
AGGAAAAGAGGCTGCAACAA+AGG - Chr3:79416570-79416589 MsG0380016129.01.T01:CDS 45.0%
AGTGCCTATTGCAAAGACAG+TGG + Chr3:79416215-79416234 None:intergenic 45.0%
CAGATAGTGAAGGGCTATGA+TGG - Chr3:79416992-79417011 MsG0380016129.01.T01:five_prime_UTR 45.0%
CATCATAGCCCTTCACTATC+TGG + Chr3:79416994-79417013 None:intergenic 45.0%
GATGAGTATCACTAGGTCCA+CGG + Chr3:79416849-79416868 None:intergenic 45.0%
TCCTGTCTTGCATCCAATGA+AGG + Chr3:79416523-79416542 None:intergenic 45.0%
TGAGTACATGGCACCTTCAT+TGG - Chr3:79416507-79416526 MsG0380016129.01.T01:intron 45.0%
! ATCACTGCAGTTACTTTCCG+TGG - Chr3:79416829-79416848 MsG0380016129.01.T01:five_prime_UTR 45.0%
! TTTTTGCACCACAAGTGGTG+TGG - Chr3:79416637-79416656 MsG0380016129.01.T01:CDS 45.0%
!! GTTGTGGAAGTACCAAAGCT+AGG - Chr3:79416550-79416569 MsG0380016129.01.T01:intron 45.0%
!!! ACAAAGGTGCTGTGTTTTGG+TGG - Chr3:79416795-79416814 MsG0380016129.01.T01:intron 45.0%
ACAAGTGGTGTGGATATGCC+TGG - Chr3:79416647-79416666 MsG0380016129.01.T01:CDS 50.0%
AGGCATATCCACACCACTTG+TGG + Chr3:79416648-79416667 None:intergenic 50.0%
ATGTCAGTCCTGCTTCTCGA+AGG + Chr3:79417021-79417040 None:intergenic 50.0%
CAGGAACATCCTTGAGGAGA+TGG + Chr3:79417045-79417064 None:intergenic 50.0%
CATCTCCTCAAGGATGTTCC+TGG - Chr3:79417043-79417062 MsG0380016129.01.T01:five_prime_UTR 50.0%
CCTATTGCAAAGACAGTGGC+AGG + Chr3:79416211-79416230 None:intergenic 50.0%
CCTGCCACTGTCTTTGCAAT+AGG - Chr3:79416208-79416227 MsG0380016129.01.T01:CDS 50.0%
GACAGTGAATGAGATACGCC+AGG - Chr3:79416169-79416188 MsG0380016129.01.T01:CDS 50.0%
GGATGCAAGACAGGATATGG+TGG - Chr3:79416528-79416547 MsG0380016129.01.T01:intron 50.0%
GGCTGCAACAAAGGCTATCA+AGG - Chr3:79416579-79416598 MsG0380016129.01.T01:CDS 50.0%
GGCTTCTCAACTTCTGGCAA+CGG + Chr3:79416935-79416954 None:intergenic 50.0%
! GCAGCCTCTTTTCCTAGCTT+TGG + Chr3:79416565-79416584 None:intergenic 50.0%
!! AGGTTGTTTTGCTGGTGGCA+CGG - Chr3:79416739-79416758 MsG0380016129.01.T01:intron 50.0%
!! ATCTGATAGTCTTGTGGGGC+AGG - Chr3:79416866-79416885 MsG0380016129.01.T01:five_prime_UTR 50.0%
!! ATGTTCTTTGAGACGAGGCC+AGG + Chr3:79417064-79417083 None:intergenic 50.0%
GATGGTCACCTTCGAGAAGC+AGG - Chr3:79417010-79417029 MsG0380016129.01.T01:five_prime_UTR 55.0%
GGTGCAGCAGCTGTGATTGT+TGG - Chr3:79416902-79416921 MsG0380016129.01.T01:five_prime_UTR 55.0%
GTGAGCTGATAGTCAGCACC+AGG + Chr3:79416668-79416687 None:intergenic 55.0%
TCTTGTGGGGCAGGCATGTT+TGG - Chr3:79416875-79416894 MsG0380016129.01.T01:five_prime_UTR 55.0%
CGAGGCCAGGAACATCCTTG+AGG + Chr3:79417051-79417070 None:intergenic 60.0%
GGCTTTCAGCTCTCTGTGCC+TGG + Chr3:79416190-79416209 None:intergenic 60.0%
GGGGCAGGCATGTTTGGAGA+TGG - Chr3:79416881-79416900 MsG0380016129.01.T01:five_prime_UTR 60.0%
TGGTGGCACGGTGCTTCGTT+TGG - Chr3:79416751-79416770 MsG0380016129.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 79416045 79417117 79416045 ID=MsG0380016129.01;Name=MsG0380016129.01
Chr3 mRNA 79416045 79417117 79416045 ID=MsG0380016129.01.T01;Parent=MsG0380016129.01;Name=MsG0380016129.01.T01;_AED=0.44;_eAED=0.44;_QI=291|0.33|0.25|1|0.66|0.75|4|0|169
Chr3 exon 79416045 79416274 79416045 ID=MsG0380016129.01.T01:exon:18013;Parent=MsG0380016129.01.T01
Chr3 exon 79416362 79416463 79416362 ID=MsG0380016129.01.T01:exon:18012;Parent=MsG0380016129.01.T01
Chr3 exon 79416555 79416728 79416555 ID=MsG0380016129.01.T01:exon:18011;Parent=MsG0380016129.01.T01
Chr3 exon 79416823 79417117 79416823 ID=MsG0380016129.01.T01:exon:18010;Parent=MsG0380016129.01.T01
Chr3 five_prime_UTR 79416827 79417117 79416827 ID=MsG0380016129.01.T01:five_prime_utr;Parent=MsG0380016129.01.T01
Chr3 CDS 79416823 79416826 79416823 ID=MsG0380016129.01.T01:cds;Parent=MsG0380016129.01.T01
Chr3 CDS 79416555 79416728 79416555 ID=MsG0380016129.01.T01:cds;Parent=MsG0380016129.01.T01
Chr3 CDS 79416362 79416463 79416362 ID=MsG0380016129.01.T01:cds;Parent=MsG0380016129.01.T01
Chr3 CDS 79416045 79416274 79416045 ID=MsG0380016129.01.T01:cds;Parent=MsG0380016129.01.T01
Gene Sequence

>MsG0380016129.01.T01

ATGTGTGATAAATCTATGATTAAGAAAAGATACATGCATTTGACTGAAGAGATTCTGAAGGGGAATCCTAGTTTATGTGAGTACATGGCACCTTCATTGGATGCAAGACAGGATATGGTGGTTGTGGAAGTACCAAAGCTAGGAAAAGAGGCTGCAACAAAGGCTATCAAGGAATGGGGTATTCGCCCGTCTGTGAAGCGTTATATGATGTATCAAAAAGGTTGTTTTGCTGGTGGCACGGTGCTTCGTTTGGCTAAAGATTTGGCTGAAAACAACAAAGGCATGTTTGGAGATGGTGCAGCAGCTGTGATTGTTGGTTCAGATCCGTTGCCAGAAGTTGAGAAGCCTTTGTTTGAATGGTTTGGACTGCACAAACAATCGTTCCAGATAGTGAAGGGCTATGATGGTCACCTTCGAGAAGCAGGACTGACATTCCATCTCCTCAAGGATGTTCCTGGCCTCGTCTCAAAGAACATTGAGAAGCTCTTGTTGAGGCATTTCAGCCTTTGA

Protein sequence

>MsG0380016129.01.T01

MCDKSMIKKRYMHLTEEILKGNPSLCEYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGIRPSVKRYMMYQKGCFAGGTVLRLAKDLAENNKGMFGDGAAAVIVGSDPLPEVEKPLFEWFGLHKQSFQIVKGYDGHLREAGLTFHLLKDVPGLVSKNIEKLLLRHFSL*