Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022680.01.T01 | XP_013457209.1 | 92.35 | 183 | 8 | 2 | 1 | 181 | 1 | 179 | 2.68E-106 | 312 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022680.01.T01 | Q9SDN0 | 36.709 | 158 | 83 | 7 | 31 | 175 | 39 | 192 | 1.74E-20 | 87 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022680.01.T01 | A0A072UZE7 | 92.350 | 183 | 8 | 2 | 1 | 181 | 1 | 179 | 1.28e-106 | 312 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048349.01 | MsG0480022680.01 | 0.802668 | 5.029772e-49 | 2.478240e-46 |
| MsG0180000437.01 | MsG0480022680.01 | 0.804131 | 2.502493e-49 | 1.280153e-46 |
| MsG0180001050.01 | MsG0480022680.01 | 0.831376 | 1.728882e-55 | 1.872734e-52 |
| MsG0180003240.01 | MsG0480022680.01 | 0.839555 | 1.477605e-57 | 2.047647e-54 |
| MsG0180005356.01 | MsG0480022680.01 | 0.804380 | 2.221451e-49 | 1.143687e-46 |
| MsG0180005357.01 | MsG0480022680.01 | 0.811117 | 8.218952e-51 | 5.044641e-48 |
| MsG0180005996.01 | MsG0480022680.01 | 0.810479 | 1.129223e-50 | 6.814234e-48 |
| MsG0180005997.01 | MsG0480022680.01 | 0.801737 | 7.819915e-49 | 3.762641e-46 |
| MsG0280011042.01 | MsG0480022680.01 | 0.829562 | 4.801828e-55 | 4.932161e-52 |
| MsG0380011613.01 | MsG0480022680.01 | 0.843435 | 1.403756e-58 | 2.196118e-55 |
| MsG0380013452.01 | MsG0480022680.01 | 0.801082 | 1.064619e-48 | 5.039049e-46 |
| MsG0480018807.01 | MsG0480022680.01 | 0.801840 | 7.446603e-49 | 3.592685e-46 |
| MsG0480022309.01 | MsG0480022680.01 | 0.801487 | 8.799331e-49 | 4.207442e-46 |
| MsG0480022507.01 | MsG0480022680.01 | 0.804300 | 2.307710e-49 | 1.185653e-46 |
| MsG0480022508.01 | MsG0480022680.01 | 0.815595 | 8.531969e-52 | 5.907874e-49 |
| MsG0480022680.01 | MsG0480022735.01 | 0.820291 | 7.413102e-53 | 5.841655e-50 |
| MsG0480022680.01 | MsG0480022736.01 | 0.858381 | 8.569237e-63 | 2.182757e-59 |
| MsG0480022680.01 | MsG0580025180.01 | 0.810779 | 9.725569e-51 | 5.916238e-48 |
| MsG0480022680.01 | MsG0580025683.01 | 0.803383 | 3.578183e-49 | 1.795536e-46 |
| MsG0480022680.01 | MsG0580025876.01 | 0.812293 | 4.559873e-51 | 2.888081e-48 |
| MsG0480022680.01 | MsG0680031429.01 | 0.800560 | 1.361259e-48 | 6.357927e-46 |
| MsG0480022680.01 | MsG0780039190.01 | 0.814365 | 1.598749e-51 | 1.070634e-48 |
| MsG0480022680.01 | MsG0780039334.01 | 0.816796 | 4.597181e-52 | 3.289461e-49 |
| MsG0480022680.01 | MsG0780039968.01 | 0.813598 | 2.360846e-51 | 1.548465e-48 |
| MsG0480022680.01 | MsG0780040480.01 | 0.819649 | 1.039672e-52 | 8.047774e-50 |
| MsG0480022680.01 | MsG0780041299.01 | 0.819224 | 1.299636e-52 | 9.941335e-50 |
| MsG0380016129.01 | MsG0480022680.01 | 0.813439 | 2.558524e-51 | 1.670898e-48 |
| MsG0380016345.01 | MsG0480022680.01 | 0.819766 | 9.775351e-53 | 7.591959e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022680.01.T01 | MTR_4g094275 | 92.350 | 183 | 8 | 2 | 1 | 181 | 1 | 179 | 3.24e-110 | 312 |
| MsG0480022680.01.T01 | MTR_2g008520 | 56.522 | 161 | 48 | 6 | 5 | 161 | 6 | 148 | 1.91e-46 | 150 |
| MsG0480022680.01.T01 | MTR_2g008540 | 56.522 | 161 | 48 | 6 | 5 | 161 | 6 | 148 | 1.91e-46 | 150 |
| MsG0480022680.01.T01 | MTR_8g081620 | 34.211 | 190 | 97 | 9 | 9 | 175 | 5 | 189 | 1.03e-21 | 87.8 |
| MsG0480022680.01.T01 | MTR_3g061170 | 42.553 | 94 | 48 | 4 | 39 | 128 | 42 | 133 | 1.85e-12 | 62.4 |
| MsG0480022680.01.T01 | MTR_2g097690 | 41.026 | 78 | 41 | 2 | 45 | 122 | 65 | 137 | 5.39e-12 | 62.0 |
| MsG0480022680.01.T01 | MTR_2g014470 | 41.250 | 80 | 42 | 3 | 53 | 130 | 2 | 78 | 8.14e-12 | 62.8 |
| MsG0480022680.01.T01 | MTR_7g085600 | 33.333 | 150 | 88 | 4 | 1 | 146 | 3 | 144 | 1.59e-11 | 62.0 |
| MsG0480022680.01.T01 | MTR_2g097690 | 41.026 | 78 | 41 | 2 | 45 | 122 | 65 | 137 | 1.82e-11 | 61.6 |
| MsG0480022680.01.T01 | MTR_5g012530 | 45.455 | 66 | 35 | 1 | 56 | 121 | 13 | 77 | 1.89e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022680.01.T01 | AT4G13830 | 36.709 | 158 | 83 | 7 | 31 | 175 | 39 | 192 | 1.78e-21 | 87.0 |
| MsG0480022680.01.T01 | AT4G13830 | 47.222 | 72 | 36 | 2 | 55 | 126 | 66 | 135 | 5.43e-14 | 66.6 |
| MsG0480022680.01.T01 | AT4G13830 | 47.222 | 72 | 36 | 2 | 55 | 126 | 66 | 135 | 8.03e-14 | 66.6 |
| MsG0480022680.01.T01 | AT3G62600 | 46.753 | 77 | 35 | 3 | 53 | 127 | 24 | 96 | 1.07e-11 | 62.8 |
| MsG0480022680.01.T01 | AT2G20560 | 37.500 | 80 | 45 | 3 | 53 | 130 | 2 | 78 | 2.00e-11 | 61.6 |
| MsG0480022680.01.T01 | AT1G28210 | 40.426 | 94 | 49 | 4 | 55 | 147 | 48 | 135 | 3.09e-11 | 61.6 |
| MsG0480022680.01.T01 | AT1G28210 | 40.426 | 94 | 49 | 4 | 55 | 147 | 48 | 135 | 3.47e-11 | 61.2 |
| MsG0480022680.01.T01 | AT1G28210 | 40.426 | 94 | 49 | 4 | 55 | 147 | 48 | 135 | 4.63e-11 | 60.8 |
| MsG0480022680.01.T01 | AT2G22360 | 33.803 | 142 | 71 | 7 | 2 | 135 | 37 | 163 | 4.64e-11 | 60.8 |
| MsG0480022680.01.T01 | AT4G28480 | 36.905 | 84 | 48 | 3 | 53 | 134 | 2 | 82 | 6.93e-11 | 60.1 |
| MsG0480022680.01.T01 | AT4G28480 | 36.905 | 84 | 48 | 3 | 53 | 134 | 2 | 82 | 8.18e-11 | 60.1 |
Find 28 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACAATATGATAGTGAATT+AGG | 0.232905 | 4:+75414694 | MsG0480022680.01.T01:CDS |
| GTAGAAATTTGATCCATTCT+TGG | 0.275553 | 4:-75414491 | None:intergenic |
| TTTAGTTGATTCCTCTTTCA+TGG | 0.279301 | 4:-75414617 | None:intergenic |
| AATGCTTAGGCTTGAGGTTA+AGG | 0.338721 | 4:-75414356 | None:intergenic |
| AGTAGACGTATGGGACAAAA+AGG | 0.403585 | 4:+75414814 | MsG0480022680.01.T01:CDS |
| ATATTGTAAATGATGAGATA+TGG | 0.436536 | 4:+75414746 | MsG0480022680.01.T01:CDS |
| AAGTGGTTGAGTTGAAGAAA+AGG | 0.458554 | 4:+75414788 | MsG0480022680.01.T01:CDS |
| ATGATGAGATATGGAGAAGT+AGG | 0.468838 | 4:+75414755 | MsG0480022680.01.T01:CDS |
| AAATTTCTACAAAATGTTGT+CGG | 0.490774 | 4:+75414504 | MsG0480022680.01.T01:CDS |
| AAAGAGAGCTTATAGATCAA+TGG | 0.492226 | 4:+75414558 | MsG0480022680.01.T01:CDS |
| GAAAAGGTCTAGTAGACGTA+TGG | 0.496414 | 4:+75414804 | MsG0480022680.01.T01:CDS |
| GCTTTGAATTTGAAATGCTT+AGG | 0.497473 | 4:-75414369 | None:intergenic |
| TCATGATCCTTCCATGAAAG+AGG | 0.504521 | 4:+75414606 | MsG0480022680.01.T01:CDS |
| ATTTGAAATGCTTAGGCTTG+AGG | 0.508030 | 4:-75414362 | None:intergenic |
| GTTGATTCCTCTTTCATGGA+AGG | 0.513452 | 4:-75414613 | None:intergenic |
| GAATCCGAAAAGCGCAACAA+TGG | 0.519653 | 4:+75414528 | MsG0480022680.01.T01:CDS |
| GAAGGATCATGACACACATC+AGG | 0.542719 | 4:-75414595 | None:intergenic |
| CTAGTGGAAGAAACCAAGAA+TGG | 0.557604 | 4:+75414478 | MsG0480022680.01.T01:CDS |
| TCATATTGTTCACGAAGCAT+AGG | 0.565520 | 4:-75414682 | None:intergenic |
| ATGTAGAGCAACAAAGCTAG+TGG | 0.571091 | 4:+75414462 | MsG0480022680.01.T01:CDS |
| GGACACAAAGCATGAAAGAT+CGG | 0.571745 | 4:+75414860 | MsG0480022680.01.T01:CDS |
| GGTCTTGGGGTAGCAGAATG+AGG | 0.576046 | 4:+75414839 | MsG0480022680.01.T01:CDS |
| AAAAGGTCTAGTAGACGTAT+GGG | 0.582302 | 4:+75414805 | MsG0480022680.01.T01:CDS |
| AGCTGTTTACTAGAGAGTTG+TGG | 0.586525 | 4:-75414406 | None:intergenic |
| AAGTAGGTGGCAAGAACAAG+TGG | 0.619538 | 4:+75414771 | MsG0480022680.01.T01:CDS |
| GTTGCAAAGTACACGTGAGT+AGG | 0.624184 | 4:-75414433 | None:intergenic |
| ATGAGATATGGAGAAGTAGG+TGG | 0.638622 | 4:+75414758 | MsG0480022680.01.T01:CDS |
| GTTTACTAGAGAGTTGTGGA+AGG | 0.676026 | 4:-75414402 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATCAACTAAAATTTTTGTT+CGG | + | Chr4:75414629-75414648 | MsG0480022680.01.T01:CDS | 15.0% |
| !! | AAATTTCTACAAAATGTTGT+CGG | + | Chr4:75414504-75414523 | MsG0480022680.01.T01:CDS | 20.0% |
| !! | ATATTGTAAATGATGAGATA+TGG | + | Chr4:75414746-75414765 | MsG0480022680.01.T01:CDS | 20.0% |
| !! | GAACAATATGATAGTGAATT+AGG | + | Chr4:75414694-75414713 | MsG0480022680.01.T01:CDS | 25.0% |
| AAAGAGAGCTTATAGATCAA+TGG | + | Chr4:75414558-75414577 | MsG0480022680.01.T01:CDS | 30.0% | |
| GTAGAAATTTGATCCATTCT+TGG | - | Chr4:75414494-75414513 | None:intergenic | 30.0% | |
| ! | GCTTTGAATTTGAAATGCTT+AGG | - | Chr4:75414372-75414391 | None:intergenic | 30.0% |
| ! | TTTAGTTGATTCCTCTTTCA+TGG | - | Chr4:75414620-75414639 | None:intergenic | 30.0% |
| AAAAGGTCTAGTAGACGTAT+GGG | + | Chr4:75414805-75414824 | MsG0480022680.01.T01:CDS | 35.0% | |
| ATGATGAGATATGGAGAAGT+AGG | + | Chr4:75414755-75414774 | MsG0480022680.01.T01:CDS | 35.0% | |
| TCATATTGTTCACGAAGCAT+AGG | - | Chr4:75414685-75414704 | None:intergenic | 35.0% | |
| ! | ATTTGAAATGCTTAGGCTTG+AGG | - | Chr4:75414365-75414384 | None:intergenic | 35.0% |
| !! | AAGTGGTTGAGTTGAAGAAA+AGG | + | Chr4:75414788-75414807 | MsG0480022680.01.T01:CDS | 35.0% |
| AATGCTTAGGCTTGAGGTTA+AGG | - | Chr4:75414359-75414378 | None:intergenic | 40.0% | |
| AGCTGTTTACTAGAGAGTTG+TGG | - | Chr4:75414409-75414428 | None:intergenic | 40.0% | |
| AGTAGACGTATGGGACAAAA+AGG | + | Chr4:75414814-75414833 | MsG0480022680.01.T01:CDS | 40.0% | |
| ATGAGATATGGAGAAGTAGG+TGG | + | Chr4:75414758-75414777 | MsG0480022680.01.T01:CDS | 40.0% | |
| ATGTAGAGCAACAAAGCTAG+TGG | + | Chr4:75414462-75414481 | MsG0480022680.01.T01:CDS | 40.0% | |
| CTAGTGGAAGAAACCAAGAA+TGG | + | Chr4:75414478-75414497 | MsG0480022680.01.T01:CDS | 40.0% | |
| GAAAAGGTCTAGTAGACGTA+TGG | + | Chr4:75414804-75414823 | MsG0480022680.01.T01:CDS | 40.0% | |
| GGACACAAAGCATGAAAGAT+CGG | + | Chr4:75414860-75414879 | MsG0480022680.01.T01:CDS | 40.0% | |
| GTTTACTAGAGAGTTGTGGA+AGG | - | Chr4:75414405-75414424 | None:intergenic | 40.0% | |
| TCATGATCCTTCCATGAAAG+AGG | + | Chr4:75414606-75414625 | MsG0480022680.01.T01:CDS | 40.0% | |
| ! | GTTGATTCCTCTTTCATGGA+AGG | - | Chr4:75414616-75414635 | None:intergenic | 40.0% |
| ! | TCTTCCATTGTTGCGCTTTT+CGG | - | Chr4:75414535-75414554 | None:intergenic | 40.0% |
| AAGTAGGTGGCAAGAACAAG+TGG | + | Chr4:75414771-75414790 | MsG0480022680.01.T01:CDS | 45.0% | |
| GAAGGATCATGACACACATC+AGG | - | Chr4:75414598-75414617 | None:intergenic | 45.0% | |
| GACGTATGGGACAAAAAGGA+AGG | + | Chr4:75414818-75414837 | MsG0480022680.01.T01:CDS | 45.0% | |
| GGACAAAAAGGAAGGTCTTG+GGG | + | Chr4:75414826-75414845 | MsG0480022680.01.T01:CDS | 45.0% | |
| GGGACAAAAAGGAAGGTCTT+GGG | + | Chr4:75414825-75414844 | MsG0480022680.01.T01:CDS | 45.0% | |
| GTTGCAAAGTACACGTGAGT+AGG | - | Chr4:75414436-75414455 | None:intergenic | 45.0% | |
| TGGGACAAAAAGGAAGGTCT+TGG | + | Chr4:75414824-75414843 | MsG0480022680.01.T01:CDS | 45.0% | |
| ! | GAATCCGAAAAGCGCAACAA+TGG | + | Chr4:75414528-75414547 | MsG0480022680.01.T01:CDS | 45.0% |
| GGTCTTGGGGTAGCAGAATG+AGG | + | Chr4:75414839-75414858 | MsG0480022680.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 75414343 | 75414888 | 75414343 | ID=MsG0480022680.01;Name=MsG0480022680.01 |
| Chr4 | mRNA | 75414343 | 75414888 | 75414343 | ID=MsG0480022680.01.T01;Parent=MsG0480022680.01;Name=MsG0480022680.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|181 |
| Chr4 | exon | 75414343 | 75414888 | 75414343 | ID=MsG0480022680.01.T01:exon:23205;Parent=MsG0480022680.01.T01 |
| Chr4 | CDS | 75414343 | 75414888 | 75414343 | ID=MsG0480022680.01.T01:cds;Parent=MsG0480022680.01.T01 |
| Gene Sequence |
| Protein sequence |
