AlfalfaGEDB | MsG0180000437.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000437.01

KEGG: K01193
GO: GO:0004553 GO:0005975
PFAM: PF00251 PF08244

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000437.01.T01	XP_003588978.1	88.385	551	26	1	1	551	39	551	0	1008

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000437.01.T01	Q43866	50.903	554	222	7	1	545	71	583	0	550

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000437.01	MsG0180000438.01	0.830540	2.772121e-55	2.929258e-52
MsG0180000437.01	MsG0180003240.01	0.820614	6.250905e-53	4.969818e-50
MsG0180000437.01	MsG0280010415.01	0.813265	2.794079e-51	1.815994e-48
MsG0180000437.01	MsG0380011613.01	0.805431	1.339196e-49	7.083803e-47
MsG0180000437.01	MsG0380011975.01	0.818834	1.593870e-52	1.206222e-49
MsG0180000437.01	MsG0380016129.01	0.814552	1.453456e-51	9.782968e-49
MsG0180000437.01	MsG0380017386.01	0.820197	7.788822e-53	6.122244e-50
MsG0180000437.01	MsG0480018807.01	0.801965	7.020951e-49	3.397828e-46
MsG0180000437.01	MsG0480019107.01	0.820195	7.797766e-53	6.128905e-50
MsG0180000437.01	MsG0480022179.01	0.805848	1.095180e-49	5.855636e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0180000437.01.T01	MTR_1g015970	88.385	551	26	1	1	551	39	551	1008
MsG0180000437.01.T01	MTR_1g015980	74.684	553	98	5	1	548	39	554	829
MsG0180000437.01.T01	MTR_1g015910	70.290	552	124	3	1	551	22	534	804
MsG0180000437.01.T01	MTR_1g015910	70.290	552	124	3	1	551	65	577	803
MsG0180000437.01.T01	MTR_1g015940	76.987	478	49	2	11	488	54	470	746
MsG0180000437.01.T01	MTR_2g099950	49.362	549	233	5	1	545	1	508	540
MsG0180000437.01.T01	MTR_2g099950	49.362	549	233	5	1	545	64	571	539
MsG0180000437.01.T01	MTR_5g066380	47.748	555	240	8	1	543	55	571	533
MsG0180000437.01.T01	MTR_8g017410	49.180	549	234	5	1	545	67	574	530
MsG0180000437.01.T01	MTR_8g017410	49.180	549	234	5	1	545	21	528	529

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0180000437.01.T01	AT3G13790	50.903	554	222	7	1	545	1	513	551
MsG0180000437.01.T01	AT3G13790	50.903	554	222	7	1	545	1	513	551
MsG0180000437.01.T01	AT3G13790	50.903	554	222	7	1	545	1	513	551
MsG0180000437.01.T01	AT3G13790	50.903	554	222	7	1	545	68	580	550
MsG0180000437.01.T01	AT3G13790	50.903	554	222	7	1	545	71	583	550
MsG0180000437.01.T01	AT1G55120	48.468	555	237	6	1	545	19	534	540
MsG0180000437.01.T01	AT1G55120	48.468	555	237	6	1	545	18	533	540
MsG0180000437.01.T01	AT1G55120	48.468	555	237	6	1	545	58	573	540
MsG0180000437.01.T01	AT1G55120	48.468	555	237	6	1	545	43	558	539
MsG0180000437.01.T01	AT5G11920	48.732	552	237	7	1	547	36	546	527

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Find 109 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGATGGGCTGGTCTTC+AGG	0.293113	1:+6004664	MsG0180000437.01.T01:CDS
AAAAGGTATCACTGCATCTC+AGG	0.460368	1:+6004949	MsG0180000437.01.T01:CDS
AAAATTTACAGGAACTTGGA+AGG	0.576790	1:+6004523	MsG0180000437.01.T01:CDS
AAACCTGCTATGTTGTATAC+AGG	0.465299	1:+6004029	MsG0180000437.01.T01:CDS
AAAGGCATGATAGGGCCATT+TGG	0.439504	1:+6005168	MsG0180000437.01.T01:CDS
AAATGGAGTTGATACAACTA+TGG	0.410792	1:+6004391	MsG0180000437.01.T01:CDS
AACATTTGGTCATGAGAAAA+TGG	0.293056	1:+6003881	MsG0180000437.01.T01:CDS
AACCCTGATGCAGCAACATT+TGG	0.398188	1:+6003867	MsG0180000437.01.T01:CDS
AACTATTTCACTTAGAAGCT+TGG	0.408959	1:+6005736	MsG0180000437.01.T01:CDS
AACTCAGCACTTTGTAGATC+AGG	0.394334	1:-6005087	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTTACAGGAACTTGGA+AGG	+	Chr1:6004523-6004542	MsG0180000437.01.T01:CDS	30.0%
AACATTTGGTCATGAGAAAA+TGG	+	Chr1:6003881-6003900	MsG0180000437.01.T01:CDS	30.0%
AACTATTTCACTTAGAAGCT+TGG	+	Chr1:6005736-6005755	MsG0180000437.01.T01:CDS	30.0%
AAGGAATTGAGATTTGACTA+TGG	+	Chr1:6004542-6004561	MsG0180000437.01.T01:CDS	30.0%
ATCGTTCGATGTTTATCTAA+TGG	-	Chr1:6005594-6005613	None:intergenic	30.0%
ATCTATCTGATCCTTACTTA+AGG	+	Chr1:6004099-6004118	MsG0180000437.01.T01:CDS	30.0%
ATCTCCAAAACTCTCTATAA+TGG	-	Chr1:6005863-6005882	None:intergenic	30.0%
ATTGAGATTTGACTATGGAA+AGG	+	Chr1:6004547-6004566	MsG0180000437.01.T01:CDS	30.0%
CGTCATGACTATTACTTTAT+TGG	+	Chr1:6004461-6004480	MsG0180000437.01.T01:CDS	30.0%
CTAAGAACAGGAGAATATTA+TGG	+	Chr1:6004600-6004619	MsG0180000437.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	6003828	6006044	6003828	ID=MsG0180000437.01;Name=MsG0180000437.01
Chr1	mRNA	6003828	6006044	6003828	ID=MsG0180000437.01.T01;Parent=MsG0180000437.01;Name=MsG0180000437.01.T01;_AED=0.46;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|6\|0\|551
Chr1	exon	6003828	6004685	6003828	ID=MsG0180000437.01.T01:exon:28051;Parent=MsG0180000437.01.T01
Chr1	exon	6004812	6004970	6004812	ID=MsG0180000437.01.T01:exon:28050;Parent=MsG0180000437.01.T01
Chr1	exon	6005060	6005298	6005060	ID=MsG0180000437.01.T01:exon:28049;Parent=MsG0180000437.01.T01
Chr1	exon	6005396	6005509	6005396	ID=MsG0180000437.01.T01:exon:28048;Parent=MsG0180000437.01.T01
Chr1	exon	6005667	6005757	6005667	ID=MsG0180000437.01.T01:exon:28047;Parent=MsG0180000437.01.T01
Chr1	exon	6005850	6006044	6005850	ID=MsG0180000437.01.T01:exon:28046;Parent=MsG0180000437.01.T01
Chr1	CDS	6003828	6004685	6003828	ID=MsG0180000437.01.T01:cds;Parent=MsG0180000437.01.T01
Chr1	CDS	6004812	6004970	6004812	ID=MsG0180000437.01.T01:cds;Parent=MsG0180000437.01.T01
Chr1	CDS	6005060	6005298	6005060	ID=MsG0180000437.01.T01:cds;Parent=MsG0180000437.01.T01
Chr1	CDS	6005396	6005509	6005396	ID=MsG0180000437.01.T01:cds;Parent=MsG0180000437.01.T01
Chr1	CDS	6005667	6005757	6005667	ID=MsG0180000437.01.T01:cds;Parent=MsG0180000437.01.T01
Chr1	CDS	6005850	6006044	6005850	ID=MsG0180000437.01.T01:cds;Parent=MsG0180000437.01.T01

Gene Sequence

>MsG0180000437.01.T01

ATGTACTATAAAGGAGTTTACCATTTATTCTACCAATATAACCCTGATGCAGCAACATTTGGTCATGAGAAAATGGTATGGGGTCATTCTATATCAAATGATCTCATAAATTGGACTCATCTAAATGATGCTATTGAACCAAGTATACCTGGTGACATAAACAGTTGTTGGTCAGGCTCAGCCACTATACTCCCAGGTGAAAAACCTGCTATGTTGTATACAGGAATTGATGAAAATAGACACCAAGTTCAAAATTTAGCCATGCCAAAGAATCTATCTGATCCTTACTTAAGGGAATGGGAAAAACATCCTCAAAATCCTTTGATGACTCCACCTAGTGGAGTTGAAGTCGGTGAATTCCGGGATCCTTCAACTGCATGGCAAGGAAATGATGGAAAATGGAGGGTAATCATTGGTGCGCAAAATGGCGATGAAGGAAAAATTATTCTTTACAAAAGTGAGGATTTTGTTAAGTGGATATTGGATCCTATACCTTTCTTTGCAACAGATGGTACTGGAGTTTGTGAATGTCCAGATTTTTTTCCTGTGTATATCAATAACACAAATGGAGTTGATACAACTATGGAAAATTCAAGCGTTAGACATGTCTTGAAGATAAGCTATCTACGTAGACGTCATGACTATTACTTTATTGGAAAATATGTATCTGATCAGGAAGAGTTTATTCCTGATGTAAAATTTACAGGAACTTGGAAGGAATTGAGATTTGACTATGGAAAGGTTTATGCTTCAAAGTCATTTTTTGACCATGCTAAGAACAGGAGAATATTATGGGCATGGGCGGAAGAGTCCGACTCTAGTGAAAATGACATTCAAAAAGGATGGGCTGGTCTTCAGACAATTCCAAGGAAGTTTTGGCTTGATAAGAATGGGAAGCAATTAATGCAATGGCCAATTGAAGAGTTAGAAAAACTCCGTGGCAATCAAATCAATATCACTGGAGAAACACTCCAAAGTGGATCAACTGTTGAAGTAAAAGGTATCACTGCATCTCAGGCTGATGTGGAAGTCTTGTTTGAGCTACCTGATCTACAAAGTGCTGAGTTGCTAGAACCAAGTGAAGTTGATCCTCAAGAACTCTGTAAAGAACAATATGCATCAAGAAAAGGCATGATAGGGCCATTTGGTTTGCAAGCTTTAGCATCAAAAGACCAGGCAGAGAGGACAACAATATCTTTCCGGATATATAGAGTCGACGATGGATTTAAATGCTTGATGATAAGTGACCAGACCAGCCACATTGTCGATGTTACAGTTCTAGATGGAAGTACATGGATAACAGAAAAGCATAGAAAGGGAAATAAAACAGAAATTGTATTTGATAATGAGAATGTTGATTTTATCAAAGGGTCTTCATTACGGGAGGACGTTGAAAAAACCTCATATGCAACTATCTTTGACATTGATCCCAATCTCAAAACTATTTCACTTAGAAGCTTGATTGACAGGTCCATTATAGAGAGTTTTGGAGATGGAGGGAGAGCTTGCATAACAAGCAGATCTTATCCTTTATTTGCTACAGAAAAAGATGCTCATCTTTATGTATTCAACAATGGAAGTCAGAATGTGGTTATCTCAGAACTGAATGCTTGGAGCATGAAGCAAGCAGAGTTTGGCACAGAAAGTATAATATAG

Protein sequence

>MsG0180000437.01.T01

MYYKGVYHLFYQYNPDAATFGHEKMVWGHSISNDLINWTHLNDAIEPSIPGDINSCWSGSATILPGEKPAMLYTGIDENRHQVQNLAMPKNLSDPYLREWEKHPQNPLMTPPSGVEVGEFRDPSTAWQGNDGKWRVIIGAQNGDEGKIILYKSEDFVKWILDPIPFFATDGTGVCECPDFFPVYINNTNGVDTTMENSSVRHVLKISYLRRRHDYYFIGKYVSDQEEFIPDVKFTGTWKELRFDYGKVYASKSFFDHAKNRRILWAWAEESDSSENDIQKGWAGLQTIPRKFWLDKNGKQLMQWPIEELEKLRGNQINITGETLQSGSTVEVKGITASQADVEVLFELPDLQSAELLEPSEVDPQELCKEQYASRKGMIGPFGLQALASKDQAERTTISFRIYRVDDGFKCLMISDQTSHIVDVTVLDGSTWITEKHRKGNKTEIVFDNENVDFIKGSSLREDVEKTSYATIFDIDPNLKTISLRSLIDRSIIESFGDGGRACITSRSYPLFATEKDAHLYVFNNGSQNVVISELNAWSMKQAEFGTESII*