Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016383.01.T01 | XP_013461292.1 | 56.835 | 139 | 14 | 4 | 31 | 168 | 100 | 193 | 1.10E-33 | 128 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016383.01.T01 | O04348 | 34.028 | 144 | 47 | 5 | 27 | 168 | 224 | 321 | 1.60E-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016383.01.T01 | A0A072V0L3 | 56.835 | 139 | 14 | 4 | 31 | 168 | 100 | 193 | 5.26e-34 | 128 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048035.01 | MsG0380016383.01 | 0.803361 | 3.615564e-49 | 1.813232e-46 |
| MsG0180003690.01 | MsG0380016383.01 | 0.803062 | 4.170677e-49 | 2.075623e-46 |
| MsG0180005172.01 | MsG0380016383.01 | 0.818327 | 2.077430e-52 | 1.550261e-49 |
| MsG0380012033.01 | MsG0380016383.01 | 0.809757 | 1.615868e-50 | 9.568493e-48 |
| MsG0380015324.01 | MsG0380016383.01 | 0.819531 | 1.106134e-52 | 8.534495e-50 |
| MsG0380015931.01 | MsG0380016383.01 | 0.817693 | 2.888882e-52 | 2.118594e-49 |
| MsG0380016383.01 | MsG0380017286.01 | 0.826785 | 2.241870e-54 | 2.123365e-51 |
| MsG0380016383.01 | MsG0380017940.01 | 0.838547 | 2.695946e-57 | 3.622957e-54 |
| MsG0380016383.01 | MsG0480018635.01 | 0.802180 | 6.339250e-49 | 3.084863e-46 |
| MsG0380016383.01 | MsG0480022479.01 | 0.804469 | 2.128411e-49 | 1.098267e-46 |
| MsG0380016383.01 | MsG0480022480.01 | 0.803005 | 4.284763e-49 | 2.129289e-46 |
| MsG0380016383.01 | MsG0480023903.01 | 0.804616 | 1.983067e-49 | 1.027159e-46 |
| MsG0380016383.01 | MsG0580027847.01 | 0.802506 | 5.430825e-49 | 2.664952e-46 |
| MsG0380016383.01 | MsG0580029906.01 | 0.801587 | 8.393714e-49 | 4.023435e-46 |
| MsG0380016383.01 | MsG0580029960.01 | 0.820665 | 6.084024e-53 | 4.843993e-50 |
| MsG0380016383.01 | MsG0780038113.01 | 0.806926 | 6.486695e-50 | 3.565222e-47 |
| MsG0380016383.01 | MsG0780038829.01 | 0.806717 | 7.181831e-50 | 3.926035e-47 |
| MsG0380016383.01 | MsG0780039249.01 | 0.844090 | 9.378107e-59 | 1.497593e-55 |
| MsG0380016383.01 | MsG0880046467.01 | 0.801233 | 9.919069e-49 | 4.712660e-46 |
| MsG0380016383.01 | MsG0880046642.01 | 0.805183 | 1.509542e-49 | 7.934129e-47 |
| MsG0280007481.01 | MsG0380016383.01 | 0.827845 | 1.249375e-54 | 1.220506e-51 |
| MsG0280010459.01 | MsG0380016383.01 | 0.802921 | 4.460358e-49 | 2.211944e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016383.01.T01 | MTR_3g088435 | 56.835 | 139 | 14 | 4 | 31 | 168 | 100 | 193 | 1.33e-37 | 128 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016383.01.T01 | AT3G24590 | 34.091 | 132 | 41 | 4 | 37 | 167 | 208 | 294 | 4.52e-12 | 63.2 |
| MsG0380016383.01.T01 | AT3G24590 | 34.091 | 132 | 41 | 4 | 37 | 167 | 189 | 275 | 4.77e-12 | 62.8 |
Find 27 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAAATGTAACTGTAAGTA+TGG | 0.316001 | 3:-82713186 | None:intergenic |
| CTAGCTATGATTCCCATATA+TGG | 0.333250 | 3:+82713693 | MsG0380016383.01.T01:CDS |
| ATACTGTACTGGTATGGATA+TGG | 0.348321 | 3:+82713151 | MsG0380016383.01.T01:CDS |
| TTATATCTCCAATGATGAAC+AGG | 0.351918 | 3:+82713925 | MsG0380016383.01.T01:CDS |
| AAAGGCCATGTTGTATTGTT+GGG | 0.363691 | 3:+82713659 | MsG0380016383.01.T01:CDS |
| AGTATAAGCAATCAATTTGT+TGG | 0.373904 | 3:-82714002 | None:intergenic |
| AACAATACAACATGGCCTTT+CGG | 0.379249 | 3:-82713656 | None:intergenic |
| AAGAGAGTTGTTGCAAAAGA+AGG | 0.419651 | 3:+82713256 | MsG0380016383.01.T01:CDS |
| GGTCTCACGGTTAATTTCGT+TGG | 0.459393 | 3:-82713537 | None:intergenic |
| AGTGGTCCCCTGTTCATCAT+TGG | 0.466911 | 3:-82713933 | None:intergenic |
| GAAAGGCCATGTTGTATTGT+TGG | 0.501484 | 3:+82713658 | MsG0380016383.01.T01:CDS |
| TGCTCAAGAGGAGGATTTCG+TGG | 0.502892 | 3:+82713500 | MsG0380016383.01.T01:CDS |
| CATGGAGGGCTCTATGTCAA+TGG | 0.528560 | 3:+82713474 | MsG0380016383.01.T01:CDS |
| TGTCAATGGTGTTGCTCAAG+AGG | 0.541257 | 3:+82713488 | MsG0380016383.01.T01:CDS |
| ATAAGACAAATAAGATCAAG+AGG | 0.568973 | 3:-82713906 | None:intergenic |
| TGTTGGTCTGTGACAGCGCA+TGG | 0.569043 | 3:-82713985 | None:intergenic |
| TGTGTGCTCGTATACTGTAC+TGG | 0.577484 | 3:+82713140 | MsG0380016383.01.T01:CDS |
| AAGGCCATGTTGTATTGTTG+GGG | 0.583522 | 3:+82713660 | MsG0380016383.01.T01:CDS |
| AATTAATAACACTGGTCTCA+CGG | 0.595411 | 3:-82713550 | None:intergenic |
| GCTCGTATACTGTACTGGTA+TGG | 0.602048 | 3:+82713145 | MsG0380016383.01.T01:CDS |
| TCCTTGCAGTATGTGCCGAA+AGG | 0.625762 | 3:+82713641 | MsG0380016383.01.T01:intron |
| TTTATTTACTACCTCAACAG+TGG | 0.625840 | 3:-82713281 | None:intergenic |
| CAATGGTGTTGCTCAAGAGG+AGG | 0.641554 | 3:+82713491 | MsG0380016383.01.T01:CDS |
| TATATCTCCAATGATGAACA+GGG | 0.661878 | 3:+82713926 | MsG0380016383.01.T01:CDS |
| AAAAGAAGGAGCCACTGTTG+AGG | 0.662509 | 3:+82713270 | MsG0380016383.01.T01:CDS |
| ATATCTCCAATGATGAACAG+GGG | 0.711901 | 3:+82713927 | MsG0380016383.01.T01:CDS |
| TTATCCCCAACAATACAACA+TGG | 0.721031 | 3:-82713664 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATTGAATAAACAGATA+AGG | - | Chr3:82713590-82713609 | None:intergenic | 15.0% |
| !! | TATCTGTTTATTCAATATTT+GGG | + | Chr3:82713590-82713609 | MsG0380016383.01.T01:intron | 15.0% |
| !! | TTATCTGTTTATTCAATATT+TGG | + | Chr3:82713589-82713608 | MsG0380016383.01.T01:intron | 15.0% |
| !!! | TCTCAAAAAGTATAAAATAA+TGG | - | Chr3:82713761-82713780 | None:intergenic | 15.0% |
| !!! | AAAGTATAAAATAATGGAGA+AGG | - | Chr3:82713755-82713774 | None:intergenic | 20.0% |
| !!! | AGAATAGTTTTACCATATAT+GGG | - | Chr3:82713708-82713727 | None:intergenic | 20.0% |
| !!! | TAGAATAGTTTTACCATATA+TGG | - | Chr3:82713709-82713728 | None:intergenic | 20.0% |
| !!! | TCACTTAAATTTTAGTATGA+TGG | - | Chr3:82713320-82713339 | None:intergenic | 20.0% |
| !!! | TTCTATTTTTAACATTTTGC+AGG | + | Chr3:82713446-82713465 | MsG0380016383.01.T01:intron | 20.0% |
| !!! | TTTTTTTTTTATGTAGCACT+TGG | + | Chr3:82713208-82713227 | MsG0380016383.01.T01:CDS | 20.0% |
| ! | ACAAAATGTAACTGTAAGTA+TGG | - | Chr3:82713189-82713208 | None:intergenic | 25.0% |
| ! | ATAAGACAAATAAGATCAAG+AGG | - | Chr3:82713909-82713928 | None:intergenic | 25.0% |
| ! | CTAAAATTTAAGTGACTACT+TGG | + | Chr3:82713325-82713344 | MsG0380016383.01.T01:intron | 25.0% |
| ! | TTGATCAGAATTAATAACAC+TGG | - | Chr3:82713561-82713580 | None:intergenic | 25.0% |
| !! | CTTCCAACTATATTTTTCAT+CGG | - | Chr3:82713960-82713979 | None:intergenic | 25.0% |
| AGAAAGTGAATGGTGTAATT+AGG | - | Chr3:82713421-82713440 | None:intergenic | 30.0% | |
| ATCATAGAAGTAATGAGGAA+AGG | - | Chr3:82713622-82713641 | None:intergenic | 30.0% | |
| ATGTGAATATGGTCTATCAA+TGG | + | Chr3:82713379-82713398 | MsG0380016383.01.T01:intron | 30.0% | |
| CTTCCGATGAAAAATATAGT+TGG | + | Chr3:82713954-82713973 | MsG0380016383.01.T01:CDS | 30.0% | |
| GCTAAGAACATATGTGAATA+TGG | + | Chr3:82713368-82713387 | MsG0380016383.01.T01:intron | 30.0% | |
| TAGAAGAAAGAGAAAGTGAA+TGG | - | Chr3:82713431-82713450 | None:intergenic | 30.0% | |
| TATATCTCCAATGATGAACA+GGG | + | Chr3:82713926-82713945 | MsG0380016383.01.T01:CDS | 30.0% | |
| TGTGAATATGGTCTATCAAT+GGG | + | Chr3:82713380-82713399 | MsG0380016383.01.T01:intron | 30.0% | |
| TTATATCTCCAATGATGAAC+AGG | + | Chr3:82713925-82713944 | MsG0380016383.01.T01:CDS | 30.0% | |
| TTTATTTACTACCTCAACAG+TGG | - | Chr3:82713284-82713303 | None:intergenic | 30.0% | |
| ! | AATTAATAACACTGGTCTCA+CGG | - | Chr3:82713553-82713572 | None:intergenic | 30.0% |
| ! | ACTATATTTTTCATCGGAAG+TGG | - | Chr3:82713954-82713973 | None:intergenic | 30.0% |
| AAAGGCCATGTTGTATTGTT+GGG | + | Chr3:82713659-82713678 | MsG0380016383.01.T01:CDS | 35.0% | |
| AACAATACAACATGGCCTTT+CGG | - | Chr3:82713659-82713678 | None:intergenic | 35.0% | |
| AAGAGAGTTGTTGCAAAAGA+AGG | + | Chr3:82713256-82713275 | MsG0380016383.01.T01:CDS | 35.0% | |
| ATATCTCCAATGATGAACAG+GGG | + | Chr3:82713927-82713946 | MsG0380016383.01.T01:CDS | 35.0% | |
| CAAGGATCATAGAAGTAATG+AGG | - | Chr3:82713627-82713646 | None:intergenic | 35.0% | |
| CTAGCTATGATTCCCATATA+TGG | + | Chr3:82713693-82713712 | MsG0380016383.01.T01:CDS | 35.0% | |
| TTATCCCCAACAATACAACA+TGG | - | Chr3:82713667-82713686 | None:intergenic | 35.0% | |
| ! | AACATTTTGCAGGTTCATCA+TGG | + | Chr3:82713456-82713475 | MsG0380016383.01.T01:intron | 35.0% |
| ! | ATACTGTACTGGTATGGATA+TGG | + | Chr3:82713151-82713170 | MsG0380016383.01.T01:CDS | 35.0% |
| ! | ATATGGTCTATCAATGGGTT+TGG | + | Chr3:82713385-82713404 | MsG0380016383.01.T01:intron | 35.0% |
| !! | TAGGCTGTTTTCTCTTCTAT+TGG | + | Chr3:82713818-82713837 | MsG0380016383.01.T01:intron | 35.0% |
| AAGGCCATGTTGTATTGTTG+GGG | + | Chr3:82713660-82713679 | MsG0380016383.01.T01:CDS | 40.0% | |
| GAAAGGCCATGTTGTATTGT+TGG | + | Chr3:82713658-82713677 | MsG0380016383.01.T01:CDS | 40.0% | |
| GATGTGCTTATAACGCAGTT+AGG | + | Chr3:82713799-82713818 | MsG0380016383.01.T01:intron | 40.0% | |
| ! | ATTTTGCAGGTTCATCATGG+AGG | + | Chr3:82713459-82713478 | MsG0380016383.01.T01:intron | 40.0% |
| ! | TTTTGCAGGTTCATCATGGA+GGG | + | Chr3:82713460-82713479 | MsG0380016383.01.T01:intron | 40.0% |
| AAAAGAAGGAGCCACTGTTG+AGG | + | Chr3:82713270-82713289 | MsG0380016383.01.T01:CDS | 45.0% | |
| GGTCTCACGGTTAATTTCGT+TGG | - | Chr3:82713540-82713559 | None:intergenic | 45.0% | |
| TGTGTGCTCGTATACTGTAC+TGG | + | Chr3:82713140-82713159 | MsG0380016383.01.T01:CDS | 45.0% | |
| ! | GCTCGTATACTGTACTGGTA+TGG | + | Chr3:82713145-82713164 | MsG0380016383.01.T01:CDS | 45.0% |
| !! | TGTCAATGGTGTTGCTCAAG+AGG | + | Chr3:82713488-82713507 | MsG0380016383.01.T01:CDS | 45.0% |
| AGTGGTCCCCTGTTCATCAT+TGG | - | Chr3:82713936-82713955 | None:intergenic | 50.0% | |
| CATGGAGGGCTCTATGTCAA+TGG | + | Chr3:82713474-82713493 | MsG0380016383.01.T01:CDS | 50.0% | |
| TCCTTGCAGTATGTGCCGAA+AGG | + | Chr3:82713641-82713660 | MsG0380016383.01.T01:intron | 50.0% | |
| TGCTCAAGAGGAGGATTTCG+TGG | + | Chr3:82713500-82713519 | MsG0380016383.01.T01:CDS | 50.0% | |
| !! | CAATGGTGTTGCTCAAGAGG+AGG | + | Chr3:82713491-82713510 | MsG0380016383.01.T01:CDS | 50.0% |
| GCCTTTCGGCACATACTGCA+AGG | - | Chr3:82713645-82713664 | None:intergenic | 55.0% | |
| ! | TGTTGGTCTGTGACAGCGCA+TGG | - | Chr3:82713988-82714007 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 82713139 | 82714013 | 82713139 | ID=MsG0380016383.01;Name=MsG0380016383.01 |
| Chr3 | mRNA | 82713139 | 82714013 | 82713139 | ID=MsG0380016383.01.T01;Parent=MsG0380016383.01;Name=MsG0380016383.01.T01;_AED=0.49;_eAED=0.62;_QI=0|0|0.25|1|0|0|4|0|168 |
| Chr3 | exon | 82713139 | 82713294 | 82713139 | ID=MsG0380016383.01.T01:exon:13919;Parent=MsG0380016383.01.T01 |
| Chr3 | exon | 82713468 | 82713560 | 82713468 | ID=MsG0380016383.01.T01:exon:13920;Parent=MsG0380016383.01.T01 |
| Chr3 | exon | 82713650 | 82713714 | 82713650 | ID=MsG0380016383.01.T01:exon:13921;Parent=MsG0380016383.01.T01 |
| Chr3 | exon | 82713821 | 82714013 | 82713821 | ID=MsG0380016383.01.T01:exon:13922;Parent=MsG0380016383.01.T01 |
| Chr3 | CDS | 82713139 | 82713294 | 82713139 | ID=MsG0380016383.01.T01:cds;Parent=MsG0380016383.01.T01 |
| Chr3 | CDS | 82713468 | 82713560 | 82713468 | ID=MsG0380016383.01.T01:cds;Parent=MsG0380016383.01.T01 |
| Chr3 | CDS | 82713650 | 82713714 | 82713650 | ID=MsG0380016383.01.T01:cds;Parent=MsG0380016383.01.T01 |
| Chr3 | CDS | 82713821 | 82714013 | 82713821 | ID=MsG0380016383.01.T01:cds;Parent=MsG0380016383.01.T01 |
| Gene Sequence |
| Protein sequence |
