AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016663.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016663.01.T01 MTR_3g092810 98.707 232 3 0 1 232 62 293 2.81e-170 470
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016663.01.T01 AT5G24460 68.075 213 60 3 26 231 88 299 9.85e-90 267

Find 73 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAGAACGTTCACAATCTT+TGG 0.212837 3:-86324601 MsG0380016663.01.T01:CDS
AGAGTGGAGGAGAGGAATTC+AGG 0.229370 3:-86324281 MsG0380016663.01.T01:CDS
CCGGAATCTGCTGCTGTTAG+AGG 0.301266 3:-86324389 MsG0380016663.01.T01:CDS
TGCTGCTGTTAGAGGGATTA+AGG 0.305461 3:-86324381 MsG0380016663.01.T01:CDS
CGTTCAGGTTGTTACGGTTC+GGG 0.323431 3:-86324522 MsG0380016663.01.T01:CDS
GGTTGTTACGGTTCGGGTTG+TGG 0.333708 3:-86324516 MsG0380016663.01.T01:CDS
TTGTGAACGTTCTTCGTTCC+GGG 0.333866 3:+86324608 None:intergenic
TCTTTGGCTTGAACTTTCTC+AGG 0.350434 3:-86324585 MsG0380016663.01.T01:CDS
ATTGTGAACGTTCTTCGTTC+CGG 0.357840 3:+86324607 None:intergenic
CCGTTCAGGTTGTTACGGTT+CGG 0.359505 3:-86324523 MsG0380016663.01.T01:CDS
CCTCTAACAGCAGCAGATTC+CGG 0.363566 3:+86324389 None:intergenic
TTGCGACGGTTATTTCTGTT+AGG 0.364535 3:+86324710 None:intergenic
TGCTACAGTGGAGGGTTTGC+CGG 0.370222 3:-86324213 MsG0380016663.01.T01:CDS
GTTATTTCTGTTAGGGTTTG+AGG 0.370357 3:+86324718 None:intergenic
GGGTCTTATCCTGGTTTGCC+TGG 0.373494 3:-86324260 MsG0380016663.01.T01:CDS
AGCTTGGGTCTGTGATTAAC+GGG 0.384878 3:-86324355 MsG0380016663.01.T01:CDS
ACCTCCGGTTCGAACCGTTC+AGG 0.390106 3:-86324537 MsG0380016663.01.T01:CDS
GTTGTTACGGTTCGGGTTGT+GGG 0.392998 3:-86324515 MsG0380016663.01.T01:CDS
ACTCTCATCTCATAAGAATT+CGG 0.424129 3:+86324299 None:intergenic
TGATAAGAAAGTTGCACATC+AGG 0.427025 3:-86324144 MsG0380016663.01.T01:CDS
GAGTGGAGGAGAGGAATTCA+GGG 0.441498 3:-86324280 MsG0380016663.01.T01:CDS
GAGCTTGGGTCTGTGATTAA+CGG 0.452338 3:-86324356 MsG0380016663.01.T01:CDS
TGCGACGGTTATTTCTGTTA+GGG 0.460191 3:+86324711 None:intergenic
AGGAATTCAGGGTCTTATCC+TGG 0.469785 3:-86324269 MsG0380016663.01.T01:CDS
GCAGATTCCGGATCCTCATT+CGG 0.471071 3:+86324401 None:intergenic
GGAGCGGGGTAGGCCGTTGT+CGG 0.474043 3:-86324435 MsG0380016663.01.T01:CDS
GGAAAGTGAGGGAGCGGGGT+AGG 0.476650 3:-86324445 MsG0380016663.01.T01:CDS
CTTCAGATTCCTTCGCCACA+TGG 0.478995 3:-86324640 MsG0380016663.01.T01:CDS
GATGAAGCCGAATGAGGATC+CGG 0.481724 3:-86324408 MsG0380016663.01.T01:CDS
AATTCTTGTGGAATCCCATC+AGG 0.484227 3:-86324480 MsG0380016663.01.T01:CDS
CGCCGCAATCTCTCTCTGAA+TGG 0.485327 3:-86324679 MsG0380016663.01.T01:CDS
TGATGGGAAAGTGAGGGAGC+GGG 0.486670 3:-86324450 MsG0380016663.01.T01:CDS
AGAGGGATTAAGGAAGAGCT+TGG 0.490718 3:-86324371 MsG0380016663.01.T01:CDS
TCGAACCGTTCAGGTTGTTA+CGG 0.491425 3:-86324528 MsG0380016663.01.T01:CDS
ATGAGATGAGAGTGGAGGAG+AGG 0.492903 3:-86324289 MsG0380016663.01.T01:CDS
GCTTGATGATTCTGAACCTC+CGG 0.496586 3:-86324552 MsG0380016663.01.T01:CDS
ACAACCTGAACGGTTCGAAC+CGG 0.503764 3:+86324533 None:intergenic
CCCATCAGACAATTCCTGAT+GGG 0.508953 3:+86324466 None:intergenic
GCCACATGGGGTGTTAAGCC+CGG 0.518471 3:-86324626 MsG0380016663.01.T01:CDS
TTCAGATTCCTTCGCCACAT+GGG 0.522542 3:-86324639 MsG0380016663.01.T01:CDS
GGCTTCATCTTCTCCGACAA+CGG 0.523703 3:+86324422 None:intergenic
AACAGAGATTTGTGATATTG+TGG 0.526664 3:-86324330 MsG0380016663.01.T01:CDS
GAGGGATTAAGGAAGAGCTT+GGG 0.532159 3:-86324370 MsG0380016663.01.T01:CDS
TCCCATCAGGAATTGTCTGA+TGG 0.535968 3:-86324467 MsG0380016663.01.T01:CDS
CGGAATCTGCTGCTGTTAGA+GGG 0.548193 3:-86324388 MsG0380016663.01.T01:CDS
CTTTGGCTTGAACTTTCTCA+GGG 0.549743 3:-86324584 MsG0380016663.01.T01:CDS
ACCTGAACGGTTCGAACCGG+AGG 0.552233 3:+86324536 None:intergenic
TCCCATCAGACAATTCCTGA+TGG 0.557523 3:+86324465 None:intergenic
CCCATCAGGAATTGTCTGAT+GGG 0.562427 3:-86324466 MsG0380016663.01.T01:CDS
CTGATGGGAAAGTGAGGGAG+CGG 0.566359 3:-86324451 MsG0380016663.01.T01:CDS
TACAGTGGAGGGTTTGCCGG+AGG 0.567653 3:-86324210 MsG0380016663.01.T01:CDS
CTTAACACCCCATGTGGCGA+AGG 0.570050 3:+86324631 None:intergenic
GATGGGAAAGTGAGGGAGCG+GGG 0.571896 3:-86324449 MsG0380016663.01.T01:CDS
ACAGTGGAGGGTTTGCCGGA+GGG 0.574179 3:-86324209 MsG0380016663.01.T01:CDS
AGCCATTCAGAGAGAGATTG+CGG 0.577556 3:+86324677 None:intergenic
ACATAACAACCAGGCAAACC+AGG 0.592229 3:+86324251 None:intergenic
TCAGATTCCTTCGCCACATG+GGG 0.592708 3:-86324638 MsG0380016663.01.T01:CDS
GGAGAAGATGAAGCCGAATG+AGG 0.599580 3:-86324414 MsG0380016663.01.T01:CDS
GTTCGGGTTGTGGGAAAAGA+CGG 0.603801 3:-86324506 MsG0380016663.01.T01:CDS
AATTGTCTGATGGGAAAGTG+AGG 0.612406 3:-86324457 MsG0380016663.01.T01:CDS
GCGGCGATGCGAAATTGCGA+CGG 0.630824 3:+86324696 None:intergenic
GGTAGATATGTTGTTAGACA+TGG 0.631652 3:+86324763 None:intergenic
ATTGTCTGATGGGAAAGTGA+GGG 0.652401 3:-86324456 MsG0380016663.01.T01:CDS
TTCTTATGAGATGAGAGTGG+AGG 0.652905 3:-86324294 MsG0380016663.01.T01:CDS
AGGTTCAGAATCATCAAGCG+AGG 0.657763 3:+86324556 None:intergenic
GAATTCTTATGAGATGAGAG+TGG 0.659927 3:-86324297 MsG0380016663.01.T01:CDS
TCCGGGCTTAACACCCCATG+TGG 0.663446 3:+86324625 None:intergenic
CCGAACCGTAACAACCTGAA+CGG 0.668287 3:+86324523 None:intergenic
TGAAGATGATGATAATGATG+AGG 0.678913 3:+86324742 None:intergenic
ATATGCAAAACATAACAACC+AGG 0.704010 3:+86324242 None:intergenic
CATGTGGCGAAGGAATCTGA+AGG 0.726001 3:+86324641 None:intergenic
GCTTGGGTCTGTGATTAACG+GGG 0.744662 3:-86324354 MsG0380016663.01.T01:CDS
AAGGAATCTGAAGGTAAACG+AGG 0.750658 3:+86324650 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGAAACATTTTTGGAAAT+GGG - Chr3:86324735-86324754 MsG0380016663.01.T01:CDS 20.0%
!!! TTAAGAAACATTTTTGGAAA+TGG - Chr3:86324734-86324753 MsG0380016663.01.T01:CDS 20.0%
!!! TTTCTGTTAAGAAACATTTT+TGG - Chr3:86324728-86324747 MsG0380016663.01.T01:CDS 20.0%
AAAAGACGGAAAAATTCTTG+TGG - Chr3:86324354-86324373 MsG0380016663.01.T01:CDS 30.0%
AACAGAGATTTGTGATATTG+TGG - Chr3:86324516-86324535 MsG0380016663.01.T01:CDS 30.0%
ACTCTCATCTCATAAGAATT+CGG + Chr3:86324550-86324569 None:intergenic 30.0%
ATATGCAAAACATAACAACC+AGG + Chr3:86324607-86324626 None:intergenic 30.0%
! TGAAGATGATGATAATGATG+AGG + Chr3:86324107-86324126 None:intergenic 30.0%
GAATTCTTATGAGATGAGAG+TGG - Chr3:86324549-86324568 MsG0380016663.01.T01:CDS 35.0%
TGATAAGAAAGTTGCACATC+AGG - Chr3:86324702-86324721 MsG0380016663.01.T01:CDS 35.0%
! ATTTTGAATGCTACAGTGGA+GGG - Chr3:86324625-86324644 MsG0380016663.01.T01:CDS 35.0%
!! GCATATTTTGAATGCTACAG+TGG - Chr3:86324621-86324640 MsG0380016663.01.T01:CDS 35.0%
!! GTTATTTCTGTTAGGGTTTG+AGG + Chr3:86324131-86324150 None:intergenic 35.0%
!! TATTTTGAATGCTACAGTGG+AGG - Chr3:86324624-86324643 MsG0380016663.01.T01:CDS 35.0%
AAGGAATCTGAAGGTAAACG+AGG + Chr3:86324199-86324218 None:intergenic 40.0%
AATTCTTGTGGAATCCCATC+AGG - Chr3:86324366-86324385 MsG0380016663.01.T01:CDS 40.0%
AATTGTCTGATGGGAAAGTG+AGG - Chr3:86324389-86324408 MsG0380016663.01.T01:CDS 40.0%
ATTGTCTGATGGGAAAGTGA+GGG - Chr3:86324390-86324409 MsG0380016663.01.T01:CDS 40.0%
ATTGTGAACGTTCTTCGTTC+CGG + Chr3:86324242-86324261 None:intergenic 40.0%
CGAAGAACGTTCACAATCTT+TGG - Chr3:86324245-86324264 MsG0380016663.01.T01:CDS 40.0%
TTCTTATGAGATGAGAGTGG+AGG - Chr3:86324552-86324571 MsG0380016663.01.T01:CDS 40.0%
! TGATTTTTGCACGTATGAGG+TGG - Chr3:86324660-86324679 MsG0380016663.01.T01:CDS 40.0%
!! CTTTGGCTTGAACTTTCTCA+GGG - Chr3:86324262-86324281 MsG0380016663.01.T01:CDS 40.0%
!! TCTTTGGCTTGAACTTTCTC+AGG - Chr3:86324261-86324280 MsG0380016663.01.T01:CDS 40.0%
!! TGCGACGGTTATTTCTGTTA+GGG + Chr3:86324138-86324157 None:intergenic 40.0%
!! TTGCGACGGTTATTTCTGTT+AGG + Chr3:86324139-86324158 None:intergenic 40.0%
ACATAACAACCAGGCAAACC+AGG + Chr3:86324598-86324617 None:intergenic 45.0%
AGAGGGATTAAGGAAGAGCT+TGG - Chr3:86324475-86324494 MsG0380016663.01.T01:CDS 45.0%
AGCCATTCAGAGAGAGATTG+CGG + Chr3:86324172-86324191 None:intergenic 45.0%
AGCTTGGGTCTGTGATTAAC+GGG - Chr3:86324491-86324510 MsG0380016663.01.T01:CDS 45.0%
AGGTTCAGAATCATCAAGCG+AGG + Chr3:86324293-86324312 None:intergenic 45.0%
CCCATCAGACAATTCCTGAT+GGG + Chr3:86324383-86324402 None:intergenic 45.0%
CCCATCAGGAATTGTCTGAT+GGG - Chr3:86324380-86324399 MsG0380016663.01.T01:CDS 45.0%
GAGCTTGGGTCTGTGATTAA+CGG - Chr3:86324490-86324509 MsG0380016663.01.T01:CDS 45.0%
GAGGGATTAAGGAAGAGCTT+GGG - Chr3:86324476-86324495 MsG0380016663.01.T01:CDS 45.0%
TACGTGCAAAAATCACCCTC+CGG + Chr3:86324655-86324674 None:intergenic 45.0%
TCCCATCAGACAATTCCTGA+TGG + Chr3:86324384-86324403 None:intergenic 45.0%
TCCCATCAGGAATTGTCTGA+TGG - Chr3:86324379-86324398 MsG0380016663.01.T01:CDS 45.0%
TGCTGCTGTTAGAGGGATTA+AGG - Chr3:86324465-86324484 MsG0380016663.01.T01:CDS 45.0%
TTCAGATTCCTTCGCCACAT+GGG - Chr3:86324207-86324226 MsG0380016663.01.T01:CDS 45.0%
TTGTGAACGTTCTTCGTTCC+GGG + Chr3:86324241-86324260 None:intergenic 45.0%
! AGGAATTCAGGGTCTTATCC+TGG - Chr3:86324577-86324596 MsG0380016663.01.T01:CDS 45.0%
! GCTTGATGATTCTGAACCTC+CGG - Chr3:86324294-86324313 MsG0380016663.01.T01:CDS 45.0%
! GGGTGATTTTTGCACGTATG+AGG - Chr3:86324657-86324676 MsG0380016663.01.T01:CDS 45.0%
!! TCGAACCGTTCAGGTTGTTA+CGG - Chr3:86324318-86324337 MsG0380016663.01.T01:CDS 45.0%
ACAACCTGAACGGTTCGAAC+CGG + Chr3:86324316-86324335 None:intergenic 50.0%
AGAGTGGAGGAGAGGAATTC+AGG - Chr3:86324565-86324584 MsG0380016663.01.T01:CDS 50.0%
ATGAGATGAGAGTGGAGGAG+AGG - Chr3:86324557-86324576 MsG0380016663.01.T01:CDS 50.0%
CATGTGGCGAAGGAATCTGA+AGG + Chr3:86324208-86324227 None:intergenic 50.0%
CCGAACCGTAACAACCTGAA+CGG + Chr3:86324326-86324345 None:intergenic 50.0%
CCTCTAACAGCAGCAGATTC+CGG + Chr3:86324460-86324479 None:intergenic 50.0%
CGGAATCTGCTGCTGTTAGA+GGG - Chr3:86324458-86324477 MsG0380016663.01.T01:CDS 50.0%
CTTCAGATTCCTTCGCCACA+TGG - Chr3:86324206-86324225 MsG0380016663.01.T01:CDS 50.0%
GAGTGGAGGAGAGGAATTCA+GGG - Chr3:86324566-86324585 MsG0380016663.01.T01:CDS 50.0%
GATGAAGCCGAATGAGGATC+CGG - Chr3:86324438-86324457 MsG0380016663.01.T01:CDS 50.0%
GCAGATTCCGGATCCTCATT+CGG + Chr3:86324448-86324467 None:intergenic 50.0%
GCTTGGGTCTGTGATTAACG+GGG - Chr3:86324492-86324511 MsG0380016663.01.T01:CDS 50.0%
GGAGAAGATGAAGCCGAATG+AGG - Chr3:86324432-86324451 MsG0380016663.01.T01:CDS 50.0%
GGCTTCATCTTCTCCGACAA+CGG + Chr3:86324427-86324446 None:intergenic 50.0%
TCAGATTCCTTCGCCACATG+GGG - Chr3:86324208-86324227 MsG0380016663.01.T01:CDS 50.0%
!! CCGTTCAGGTTGTTACGGTT+CGG - Chr3:86324323-86324342 MsG0380016663.01.T01:CDS 50.0%
!! CGTTCAGGTTGTTACGGTTC+GGG - Chr3:86324324-86324343 MsG0380016663.01.T01:CDS 50.0%
!! GTTCGGGTTGTGGGAAAAGA+CGG - Chr3:86324340-86324359 MsG0380016663.01.T01:CDS 50.0%
!! GTTGTTACGGTTCGGGTTGT+GGG - Chr3:86324331-86324350 MsG0380016663.01.T01:CDS 50.0%
CCGGAATCTGCTGCTGTTAG+AGG - Chr3:86324457-86324476 MsG0380016663.01.T01:CDS 55.0%
CGCCGCAATCTCTCTCTGAA+TGG - Chr3:86324167-86324186 MsG0380016663.01.T01:CDS 55.0%
CTGATGGGAAAGTGAGGGAG+CGG - Chr3:86324395-86324414 MsG0380016663.01.T01:CDS 55.0%
CTTAACACCCCATGTGGCGA+AGG + Chr3:86324218-86324237 None:intergenic 55.0%
TGATGGGAAAGTGAGGGAGC+GGG - Chr3:86324396-86324415 MsG0380016663.01.T01:CDS 55.0%
TGCTACAGTGGAGGGTTTGC+CGG - Chr3:86324633-86324652 MsG0380016663.01.T01:CDS 55.0%
! GGGTCTTATCCTGGTTTGCC+TGG - Chr3:86324586-86324605 MsG0380016663.01.T01:CDS 55.0%
!! GGTTGTTACGGTTCGGGTTG+TGG - Chr3:86324330-86324349 MsG0380016663.01.T01:CDS 55.0%
ACAGTGGAGGGTTTGCCGGA+GGG - Chr3:86324637-86324656 MsG0380016663.01.T01:CDS 60.0%
ACCTGAACGGTTCGAACCGG+AGG + Chr3:86324313-86324332 None:intergenic 60.0%
GATGGGAAAGTGAGGGAGCG+GGG - Chr3:86324397-86324416 MsG0380016663.01.T01:CDS 60.0%
GCGGCGATGCGAAATTGCGA+CGG + Chr3:86324153-86324172 None:intergenic 60.0%
TACAGTGGAGGGTTTGCCGG+AGG - Chr3:86324636-86324655 MsG0380016663.01.T01:CDS 60.0%
TCCGGGCTTAACACCCCATG+TGG + Chr3:86324224-86324243 None:intergenic 60.0%
! ACCTCCGGTTCGAACCGTTC+AGG - Chr3:86324309-86324328 MsG0380016663.01.T01:CDS 60.0%
! GCCACATGGGGTGTTAAGCC+CGG - Chr3:86324220-86324239 MsG0380016663.01.T01:CDS 60.0%
GGAAAGTGAGGGAGCGGGGT+AGG - Chr3:86324401-86324420 MsG0380016663.01.T01:CDS 65.0%
GGAGCGGGGTAGGCCGTTGT+CGG - Chr3:86324411-86324430 MsG0380016663.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 86324085 86324783 86324085 ID=MsG0380016663.01;Name=MsG0380016663.01
Chr3 mRNA 86324085 86324783 86324085 ID=MsG0380016663.01.T01;Parent=MsG0380016663.01;Name=MsG0380016663.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|232
Chr3 exon 86324085 86324783 86324085 ID=MsG0380016663.01.T01:exon:15808;Parent=MsG0380016663.01.T01
Chr3 CDS 86324085 86324783 86324085 ID=MsG0380016663.01.T01:cds;Parent=MsG0380016663.01.T01
Gene Sequence

>MsG0380016663.01.T01

ATGTCTAACAACATATCTACCTCATCATTATCATCATCTTCATCCTCAAACCCTAACAGAAATAACCGTCGCAATTTCGCATCGCCGCAATCTCTCTCTGAATGGCTTAAACCTCGTTTACCTTCAGATTCCTTCGCCACATGGGGTGTTAAGCCCGGAACGAAGAACGTTCACAATCTTTGGCTTGAACTTTCTCAGGGAGAAACCTCGCTTGATGATTCTGAACCTCCGGTTCGAACCGTTCAGGTTGTTACGGTTCGGGTTGTGGGAAAAGACGGAAAAATTCTTGTGGAATCCCATCAGGAATTGTCTGATGGGAAAGTGAGGGAGCGGGGTAGGCCGTTGTCGGAGAAGATGAAGCCGAATGAGGATCCGGAATCTGCTGCTGTTAGAGGGATTAAGGAAGAGCTTGGGTCTGTGATTAACGGGGAAACAGAGATTTGTGATATTGTGGCGATTGATCCGAATTCTTATGAGATGAGAGTGGAGGAGAGGAATTCAGGGTCTTATCCTGGTTTGCCTGGTTGTTATGTTTTGCATATTTTGAATGCTACAGTGGAGGGTTTGCCGGAGGGTGATTTTTGCACGTATGAGGTGGACGAGTATGATGATTCTGATGATAAGAAAGTTGCACATCAGGCTGTTTCTGTTAAGAAACATTTTTGGAAATGGGTTAGTGCTGATTCTATTCAACCTTGA

Protein sequence

>MsG0380016663.01.T01

MSNNISTSSLSSSSSSNPNRNNRRNFASPQSLSEWLKPRLPSDSFATWGVKPGTKNVHNLWLELSQGETSLDDSEPPVRTVQVVTVRVVGKDGKILVESHQELSDGKVRERGRPLSEKMKPNEDPESAAVRGIKEELGSVINGETEICDIVAIDPNSYEMRVEERNSGSYPGLPGCYVLHILNATVEGLPEGDFCTYEVDEYDDSDDKKVAHQAVSVKKHFWKWVSADSIQP*