AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013999.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013999.01.T01 MTR_2g062930 93.939 66 4 0 1 66 1 66 6.18e-40 127
MsG0380013999.01.T01 MTR_4g051375 98.039 51 1 0 1 51 1 51 1.82e-31 105
MsG0380013999.01.T01 MTR_1g007850 72.727 33 9 0 33 65 20 52 5.96e-11 53.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013999.01.T01 ATCG00710 90.141 71 7 0 1 71 1 71 9.82e-42 130

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTCTCTAATTAGAAATT+TGG 0.201810 3:+48616011 None:intergenic
AGAAATTTGGTCCAGTAAAA+TGG 0.222192 3:+48616024 None:intergenic
AAACCGTTGAATTCAGAATA+TGG 0.285183 3:-48616145 MsG0380013999.01.T01:CDS
ATGGGGAACTACCCCTTTGA+TGG 0.408879 3:-48616107 MsG0380013999.01.T01:CDS
GGTCCAAGACGAACTGTTGT+AGG 0.449268 3:-48616178 MsG0380013999.01.T01:CDS
CCCTTTGATGGGTATTGCAA+TGG 0.456957 3:-48616095 MsG0380013999.01.T01:CDS
TATTGCAATGGCTCTATTTG+CGG 0.465025 3:-48616083 MsG0380013999.01.T01:CDS
AGAATATGGTAAAGTAGCTC+CGG 0.467064 3:-48616131 MsG0380013999.01.T01:CDS
CCATTGCAATACCCATCAAA+GGG 0.506208 3:+48616095 None:intergenic
ATGGCTACACAAACCGTTGA+GGG 0.512439 3:-48616214 MsG0380013999.01.T01:CDS
GTTGAGGGTAGTTCTAGATC+TGG 0.522119 3:-48616199 MsG0380013999.01.T01:CDS
TGGTAAAGTAGCTCCGGGAT+GGG 0.527741 3:-48616125 MsG0380013999.01.T01:CDS
TCTCCTACAACAGTTCGTCT+TGG 0.532181 3:+48616175 None:intergenic
GCCATTGCAATACCCATCAA+AGG 0.539340 3:+48616094 None:intergenic
GGTAAAGTAGCTCCGGGATG+GGG 0.547587 3:-48616124 MsG0380013999.01.T01:CDS
TTACCATATTCTGAATTCAA+CGG 0.547816 3:+48616142 None:intergenic
TGGGGAACTACCCCTTTGAT+GGG 0.558023 3:-48616106 MsG0380013999.01.T01:CDS
ATGGTAAAGTAGCTCCGGGA+TGG 0.560142 3:-48616126 MsG0380013999.01.T01:CDS
GAATATGGTAAAGTAGCTCC+GGG 0.573000 3:-48616130 MsG0380013999.01.T01:CDS
AAAGGGGTAGTTCCCCATCC+CGG 0.593845 3:+48616112 None:intergenic
TATGGCTACACAAACCGTTG+AGG 0.620476 3:-48616215 MsG0380013999.01.T01:CDS
AGATCTAGAACTACCCTCAA+CGG 0.642132 3:+48616201 None:intergenic
CATTGCAATACCCATCAAAG+GGG 0.733375 3:+48616096 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTACCATATTCTGAATTCAA+CGG + Chr3:48616093-48616112 None:intergenic 25.0%
!! TTATAATTCGTCCATTTTAC+TGG - Chr3:48616197-48616216 MsG0380013999.01.T01:CDS 25.0%
AAACCGTTGAATTCAGAATA+TGG - Chr3:48616087-48616106 MsG0380013999.01.T01:CDS 30.0%
AGAAATTTGGTCCAGTAAAA+TGG + Chr3:48616211-48616230 None:intergenic 30.0%
AGAATATGGTAAAGTAGCTC+CGG - Chr3:48616101-48616120 MsG0380013999.01.T01:CDS 35.0%
! TATTGCAATGGCTCTATTTG+CGG - Chr3:48616149-48616168 MsG0380013999.01.T01:CDS 35.0%
CATTGCAATACCCATCAAAG+GGG + Chr3:48616139-48616158 None:intergenic 40.0%
CCATTGCAATACCCATCAAA+GGG + Chr3:48616140-48616159 None:intergenic 40.0%
GAATATGGTAAAGTAGCTCC+GGG - Chr3:48616102-48616121 MsG0380013999.01.T01:CDS 40.0%
! AGATCTAGAACTACCCTCAA+CGG + Chr3:48616034-48616053 None:intergenic 40.0%
ATGGCTACACAAACCGTTGA+GGG - Chr3:48616018-48616037 MsG0380013999.01.T01:CDS 45.0%
GCCATTGCAATACCCATCAA+AGG + Chr3:48616141-48616160 None:intergenic 45.0%
TCTCCTACAACAGTTCGTCT+TGG + Chr3:48616060-48616079 None:intergenic 45.0%
! CCCTTTGATGGGTATTGCAA+TGG - Chr3:48616137-48616156 MsG0380013999.01.T01:CDS 45.0%
!! GTTGAGGGTAGTTCTAGATC+TGG - Chr3:48616033-48616052 MsG0380013999.01.T01:CDS 45.0%
ATGGGGAACTACCCCTTTGA+TGG - Chr3:48616125-48616144 MsG0380013999.01.T01:CDS 50.0%
ATGGTAAAGTAGCTCCGGGA+TGG - Chr3:48616106-48616125 MsG0380013999.01.T01:CDS 50.0%
GGTCCAAGACGAACTGTTGT+AGG - Chr3:48616054-48616073 MsG0380013999.01.T01:CDS 50.0%
TGGGGAACTACCCCTTTGAT+GGG - Chr3:48616126-48616145 MsG0380013999.01.T01:CDS 50.0%
TGGTAAAGTAGCTCCGGGAT+GGG - Chr3:48616107-48616126 MsG0380013999.01.T01:CDS 50.0%
AAAGGGGTAGTTCCCCATCC+CGG + Chr3:48616123-48616142 None:intergenic 55.0%
GGTAAAGTAGCTCCGGGATG+GGG - Chr3:48616108-48616127 MsG0380013999.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48616018 48616236 48616018 ID=MsG0380013999.01;Name=MsG0380013999.01
Chr3 mRNA 48616018 48616236 48616018 ID=MsG0380013999.01.T01;Parent=MsG0380013999.01;Name=MsG0380013999.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|72
Chr3 exon 48616018 48616236 48616018 ID=MsG0380013999.01.T01:exon:56794;Parent=MsG0380013999.01.T01
Chr3 CDS 48616018 48616236 48616018 ID=MsG0380013999.01.T01:cds;Parent=MsG0380013999.01.T01
Gene Sequence

>MsG0380013999.01.T01

ATGGCTACACAAACCGTTGAGGGTAGTTCTAGATCTGGTCCAAGACGAACTGTTGTAGGAGATTTATTGAAACCGTTGAATTCAGAATATGGTAAAGTAGCTCCGGGATGGGGAACTACCCCTTTGATGGGTATTGCAATGGCTCTATTTGCGGTATTTCTCTCTATTATTTTAGAGATTTATAATTCGTCCATTTTACTGGACCAAATTTCTAATTAG

Protein sequence

>MsG0380013999.01.T01

MATQTVEGSSRSGPRRTVVGDLLKPLNSEYGKVAPGWGTTPLMGIAMALFAVFLSIILEIYNSSILLDQISN*