AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041121.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041121.01.T01 MTR_7g104540 64.198 162 15 2 1 119 1 162 4.60e-63 190
MsG0780041121.01.T01 MTR_1g059990 38.400 125 63 5 1 121 1 115 9.98e-18 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041121.01.T01 AT3G52740 44.167 120 46 5 15 119 12 125 6.78e-20 79.7

Find 27 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTTATCATCATGGTTT+TGG 0.183603 7:-86860735 None:intergenic
TCCTCTTGACCCCATATTTC+AGG 0.201417 7:-86860829 None:intergenic
AAGTTTGGATTCCTGAAATA+TGG 0.218786 7:+86860818 MsG0780041121.01.T01:CDS
CACTATGGCACGTGCTGCTT+TGG 0.339934 7:+86860912 MsG0780041121.01.T01:CDS
ACACTATTGCAAGAGAAGTT+TGG 0.344019 7:+86860803 MsG0780041121.01.T01:CDS
GCTCTTTATCTTTATCATCA+TGG 0.366832 7:-86860741 None:intergenic
AAGAGGATTTATTGAAGGAT+TGG 0.393645 7:+86860845 MsG0780041121.01.T01:CDS
AGTTTGGATTCCTGAAATAT+GGG 0.427180 7:+86860819 MsG0780041121.01.T01:CDS
AGTTCCAAGCAAGATCACTA+TGG 0.434204 7:+86860897 MsG0780041121.01.T01:CDS
CCTTGTTTGCAAAGAAGAGA+TGG 0.451863 7:-86860773 None:intergenic
GGGTCAAGAGGATTTATTGA+AGG 0.475977 7:+86860840 MsG0780041121.01.T01:CDS
CAAGATTCCTTCACACCCAT+TGG 0.482903 7:+86860630 MsG0780041121.01.T01:CDS
GTGATCTTGCTTGGAACTAA+AGG 0.483762 7:-86860892 None:intergenic
CTCATCTAAAGATGTCAACT+TGG 0.490619 7:+86860669 MsG0780041121.01.T01:CDS
CCATCTCTTCTTTGCAAACA+AGG 0.494196 7:+86860773 MsG0780041121.01.T01:CDS
CTTGTTTGCAAAGAAGAGAT+GGG 0.508262 7:-86860772 None:intergenic
CATCAGATTCAACAGAGGAT+TGG 0.517512 7:-86860606 None:intergenic
CGTGCCATAGTGATCTTGCT+TGG 0.523167 7:-86860901 None:intergenic
TTTGAGTCCAATGGGTGTGA+AGG 0.539347 7:-86860637 None:intergenic
GTTGACATCTTTAGATGAGT+GGG 0.544029 7:-86860665 None:intergenic
TCCTGAAATATGGGGTCAAG+AGG 0.551314 7:+86860828 MsG0780041121.01.T01:CDS
AGTTGACATCTTTAGATGAG+TGG 0.576084 7:-86860666 None:intergenic
CGTGCTGCTTTGGTTGAAGA+GGG 0.579273 7:+86860922 MsG0780041121.01.T01:CDS
ACGTGCTGCTTTGGTTGAAG+AGG 0.593330 7:+86860921 MsG0780041121.01.T01:CDS
TTGACATCTTTAGATGAGTG+GGG 0.627794 7:-86860664 None:intergenic
GTTTGGATTCCTGAAATATG+GGG 0.638334 7:+86860820 MsG0780041121.01.T01:CDS
CTTGTCATCAGATTCAACAG+AGG 0.696844 7:-86860611 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTTTATCATCATGGTTT+TGG - Chr7:86860738-86860757 None:intergenic 25.0%
AAGAGGATTTATTGAAGGAT+TGG + Chr7:86860845-86860864 MsG0780041121.01.T01:CDS 30.0%
AAGTTTGGATTCCTGAAATA+TGG + Chr7:86860818-86860837 MsG0780041121.01.T01:CDS 30.0%
AGTTTGGATTCCTGAAATAT+GGG + Chr7:86860819-86860838 MsG0780041121.01.T01:CDS 30.0%
GCTCTTTATCTTTATCATCA+TGG - Chr7:86860744-86860763 None:intergenic 30.0%
TTTGAGTGTTCAAAAAAGGA+GGG - Chr7:86860711-86860730 None:intergenic 30.0%
!! ACCTTTTGAGTGTTCAAAAA+AGG - Chr7:86860715-86860734 None:intergenic 30.0%
!! TTTTGAGTGTTCAAAAAAGG+AGG - Chr7:86860712-86860731 None:intergenic 30.0%
!!! TCCTTTTTTGAACACTCAAA+AGG + Chr7:86860711-86860730 MsG0780041121.01.T01:CDS 30.0%
ACACTATTGCAAGAGAAGTT+TGG + Chr7:86860803-86860822 MsG0780041121.01.T01:CDS 35.0%
AGTTGACATCTTTAGATGAG+TGG - Chr7:86860669-86860688 None:intergenic 35.0%
CTCATCTAAAGATGTCAACT+TGG + Chr7:86860669-86860688 MsG0780041121.01.T01:CDS 35.0%
CTTGTTTGCAAAGAAGAGAT+GGG - Chr7:86860775-86860794 None:intergenic 35.0%
GTTGACATCTTTAGATGAGT+GGG - Chr7:86860668-86860687 None:intergenic 35.0%
GTTTGGATTCCTGAAATATG+GGG + Chr7:86860820-86860839 MsG0780041121.01.T01:CDS 35.0%
TTGACATCTTTAGATGAGTG+GGG - Chr7:86860667-86860686 None:intergenic 35.0%
AGTTCCAAGCAAGATCACTA+TGG + Chr7:86860897-86860916 MsG0780041121.01.T01:CDS 40.0%
CATCAGATTCAACAGAGGAT+TGG - Chr7:86860609-86860628 None:intergenic 40.0%
CCATCTCTTCTTTGCAAACA+AGG + Chr7:86860773-86860792 MsG0780041121.01.T01:CDS 40.0%
CTTGTCATCAGATTCAACAG+AGG - Chr7:86860614-86860633 None:intergenic 40.0%
GGGTCAAGAGGATTTATTGA+AGG + Chr7:86860840-86860859 MsG0780041121.01.T01:CDS 40.0%
GTGATCTTGCTTGGAACTAA+AGG - Chr7:86860895-86860914 None:intergenic 40.0%
! CCTTGTTTGCAAAGAAGAGA+TGG - Chr7:86860776-86860795 None:intergenic 40.0%
!!! GGGGTTTTTTTGAGTCCAAT+GGG - Chr7:86860648-86860667 None:intergenic 40.0%
!!! TGGGGTTTTTTTGAGTCCAA+TGG - Chr7:86860649-86860668 None:intergenic 40.0%
CAAGATTCCTTCACACCCAT+TGG + Chr7:86860630-86860649 MsG0780041121.01.T01:CDS 45.0%
TCCTCTTGACCCCATATTTC+AGG - Chr7:86860832-86860851 None:intergenic 45.0%
TCCTGAAATATGGGGTCAAG+AGG + Chr7:86860828-86860847 MsG0780041121.01.T01:CDS 45.0%
TTTGAGTCCAATGGGTGTGA+AGG - Chr7:86860640-86860659 None:intergenic 45.0%
CGTGCCATAGTGATCTTGCT+TGG - Chr7:86860904-86860923 None:intergenic 50.0%
!! ACGTGCTGCTTTGGTTGAAG+AGG + Chr7:86860921-86860940 MsG0780041121.01.T01:CDS 50.0%
!! CGTGCTGCTTTGGTTGAAGA+GGG + Chr7:86860922-86860941 MsG0780041121.01.T01:CDS 50.0%
!! CACTATGGCACGTGCTGCTT+TGG + Chr7:86860912-86860931 MsG0780041121.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 86860598 86860969 86860598 ID=MsG0780041121.01;Name=MsG0780041121.01
Chr7 mRNA 86860598 86860969 86860598 ID=MsG0780041121.01.T01;Parent=MsG0780041121.01;Name=MsG0780041121.01.T01;_AED=0.52;_eAED=0.52;_QI=0|-1|0|1|-1|1|1|0|123
Chr7 exon 86860598 86860969 86860598 ID=MsG0780041121.01.T01:exon:11951;Parent=MsG0780041121.01.T01
Chr7 CDS 86860598 86860969 86860598 ID=MsG0780041121.01.T01:cds;Parent=MsG0780041121.01.T01
Gene Sequence

>MsG0780041121.01.T01

ATGTCTTACCAATCCTCTGTTGAATCTGATGACAAGATTCCTTCACACCCATTGGACTCAAAAAAACCCCACTCATCTAAAGATGTCAACTTGGAGACAAAACAAGATGACCCTCCTTTTTTGAACACTCAAAAGGTCCAAAACCATGATGATAAAGATAAAGAGCACACAAAACCCATCTCTTCTTTGCAAACAAGGAGAAATGACACTATTGCAAGAGAAGTTTGGATTCCTGAAATATGGGGTCAAGAGGATTTATTGAAGGATTGGATAGATTGCACTGCATTTGATGCTCCTTTAGTTCCAAGCAAGATCACTATGGCACGTGCTGCTTTGGTTGAAGAGGGTACTAAAACAATGCTGCTCCTCTAA

Protein sequence

>MsG0780041121.01.T01

MSYQSSVESDDKIPSHPLDSKKPHSSKDVNLETKQDDPPFLNTQKVQNHDDKDKEHTKPISSLQTRRNDTIAREVWIPEIWGQEDLLKDWIDCTAFDAPLVPSKITMARAALVEEGTKTMLLL*