AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010617.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010617.01.T01 MTR_2g088770 76.000 50 12 0 1 50 261 310 7.08e-20 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010617.01.T01 AT1G05260 50.000 50 25 0 1 50 261 310 3.70e-11 56.2

Find 10 sgRNAs with CRISPR-Local

Find 8 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAAATCAATGGAGAAAA+TGG 0.079582 2:+72342789 MsG0280010617.01.T01:CDS
AAGTTTCAAGTGAGCCTTGA+AGG 0.403365 2:-72342753 None:intergenic
CAGGGTCACAAGGTGTGATT+AGG 0.404815 2:+72342831 MsG0280010617.01.T01:CDS
CTTGTGAATCAATTCCTTCA+AGG 0.416074 2:+72342739 MsG0280010617.01.T01:CDS
TGCTGAATTTGCAAAATCAA+TGG 0.452311 2:+72342780 MsG0280010617.01.T01:CDS
GCAAAATCAATGGAGAAAAT+GGG 0.466453 2:+72342790 MsG0280010617.01.T01:CDS
GACAAGTTAAAGTTCAAGAC+AGG 0.492085 2:+72342812 MsG0280010617.01.T01:CDS
AGTCAAGTTGTCAAGAGAAG+AGG 0.538282 2:+72342668 None:intergenic
AAGTTCAAGACAGGGTCACA+AGG 0.640192 2:+72342821 MsG0280010617.01.T01:CDS
ACAAGTTAAAGTTCAAGACA+GGG 0.710888 2:+72342813 MsG0280010617.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAAGTTAAAGTTCAAGACA+GGG + Chr2:72342813-72342832 MsG0280010617.01.T01:CDS 30.0%
GCAAAATCAATGGAGAAAAT+GGG + Chr2:72342790-72342809 MsG0280010617.01.T01:CDS 30.0%
TGCAAAATCAATGGAGAAAA+TGG + Chr2:72342789-72342808 MsG0280010617.01.T01:CDS 30.0%
TGCTGAATTTGCAAAATCAA+TGG + Chr2:72342780-72342799 MsG0280010617.01.T01:CDS 30.0%
CTTGTGAATCAATTCCTTCA+AGG + Chr2:72342739-72342758 MsG0280010617.01.T01:CDS 35.0%
GACAAGTTAAAGTTCAAGAC+AGG + Chr2:72342812-72342831 MsG0280010617.01.T01:CDS 35.0%
! AAGTTTCAAGTGAGCCTTGA+AGG - Chr2:72342756-72342775 None:intergenic 40.0%
AAGTTCAAGACAGGGTCACA+AGG + Chr2:72342821-72342840 MsG0280010617.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr2 gene 72342679 72342852 72342679 ID=MsG0280010617.01;Name=MsG0280010617.01
Chr2 mRNA 72342679 72342852 72342679 ID=MsG0280010617.01.T01;Parent=MsG0280010617.01;Name=MsG0280010617.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|57
Chr2 exon 72342679 72342852 72342679 ID=MsG0280010617.01.T01:exon:19576;Parent=MsG0280010617.01.T01
Chr2 CDS 72342679 72342852 72342679 ID=MsG0280010617.01.T01:cds;Parent=MsG0280010617.01.T01
Gene Sequence

>MsG0280010617.01.T01

CAAGAGAAGAGGTTTGTTCAGTCTGATTCTGCATTGTTGACAAATTCAGTTACAAAAGCTCTTGTGAATCAATTCCTTCAAGGCTCACTTGAAACTTTCTATGCTGAATTTGCAAAATCAATGGAGAAAATGGGACAAGTTAAAGTTCAAGACAGGGTCACAAGGTGTGATTAG

Protein sequence

>MsG0280010617.01.T01

QEKRFVQSDSALLTNSVTKALVNQFLQGSLETFYAEFAKSMEKMGQVKVQDRVTRCD*