Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016883.01.T01 | XP_003602697.2 | 84.978 | 446 | 10 | 3 | 1 | 415 | 1 | 420 | 0 | 743 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003510.01 | MsG0380016883.01 | 0.813949 | 1.975236e-51 | 1.307871e-48 |
| MsG0180003815.01 | MsG0380016883.01 | 0.813865 | 2.061673e-51 | 1.362019e-48 |
| MsG0180005982.01 | MsG0380016883.01 | 0.829452 | 5.107710e-55 | 5.229618e-52 |
| MsG0280006634.01 | MsG0380016883.01 | 0.878078 | 3.755158e-69 | 1.933765e-65 |
| MsG0280006669.01 | MsG0380016883.01 | 0.829117 | 6.159367e-55 | 6.244633e-52 |
| MsG0280006708.01 | MsG0380016883.01 | 0.806835 | 6.780620e-50 | 3.718139e-47 |
| MsG0280011175.01 | MsG0380016883.01 | 0.802108 | 6.560521e-49 | 3.186718e-46 |
| MsG0380013467.01 | MsG0380016883.01 | 0.815406 | 9.395245e-52 | 6.471863e-49 |
| MsG0380016740.01 | MsG0380016883.01 | 0.812959 | 3.260139e-51 | 2.101405e-48 |
| MsG0380016883.01 | MsG0380017891.01 | -0.802316 | 5.945304e-49 | 2.903424e-46 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016883.01.T01 | MTR_3g097060 | 84.978 | 446 | 10 | 3 | 1 | 415 | 1 | 420 | 0.0 | 743 |
| MsG0380016883.01.T01 | MTR_3g097060 | 83.538 | 407 | 10 | 3 | 1 | 376 | 1 | 381 | 0.0 | 660 |
| MsG0380016883.01.T01 | MTR_3g096780 | 31.496 | 381 | 180 | 11 | 39 | 413 | 39 | 344 | 6.03e-45 | 161 |
| MsG0380016883.01.T01 | MTR_3g096780 | 27.744 | 328 | 176 | 9 | 39 | 341 | 92 | 383 | 2.82e-22 | 98.6 |
| MsG0380016883.01.T01 | MTR_8g094410 | 28.497 | 386 | 189 | 10 | 39 | 415 | 37 | 344 | 2.58e-35 | 135 |
| MsG0380016883.01.T01 | MTR_8g094410 | 28.721 | 383 | 192 | 11 | 39 | 412 | 90 | 400 | 2.07e-31 | 124 |
| MsG0380016883.01.T01 | MTR_8g094410 | 27.666 | 347 | 176 | 9 | 68 | 411 | 14 | 288 | 3.82e-25 | 106 |
| MsG0380016883.01.T01 | MTR_8g094410 | 28.497 | 386 | 189 | 10 | 39 | 415 | 37 | 344 | 3.92e-35 | 135 |
| MsG0380016883.01.T01 | MTR_8g094410 | 29.661 | 354 | 175 | 10 | 39 | 390 | 90 | 371 | 4.49e-31 | 124 |
| MsG0380016883.01.T01 | MTR_8g094410 | 27.666 | 347 | 176 | 9 | 68 | 411 | 14 | 288 | 7.38e-25 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016883.01.T01 | AT5G08710 | 57.619 | 420 | 122 | 3 | 25 | 414 | 40 | 433 | 5.92e-158 | 452 |
| MsG0380016883.01.T01 | AT5G08710 | 54.859 | 319 | 88 | 3 | 25 | 313 | 40 | 332 | 4.03e-103 | 309 |
| MsG0380016883.01.T01 | AT5G63860 | 29.630 | 378 | 192 | 10 | 39 | 413 | 39 | 345 | 6.63e-43 | 156 |
| MsG0380016883.01.T01 | AT5G63860 | 29.915 | 351 | 173 | 9 | 39 | 387 | 92 | 371 | 4.06e-33 | 129 |
| MsG0380016883.01.T01 | AT5G12350 | 26.835 | 395 | 183 | 14 | 68 | 411 | 309 | 648 | 1.49e-24 | 107 |
| MsG0380016883.01.T01 | AT5G12350 | 30.088 | 226 | 116 | 6 | 189 | 413 | 298 | 482 | 2.53e-19 | 91.3 |
| MsG0380016883.01.T01 | AT5G12350 | 38.400 | 125 | 71 | 3 | 32 | 154 | 496 | 616 | 1.23e-13 | 73.6 |
| MsG0380016883.01.T01 | AT5G19420 | 25.641 | 390 | 194 | 9 | 68 | 411 | 313 | 652 | 3.85e-24 | 105 |
| MsG0380016883.01.T01 | AT5G19420 | 29.646 | 226 | 117 | 6 | 189 | 413 | 302 | 486 | 1.32e-18 | 89.0 |
| MsG0380016883.01.T01 | AT5G19420 | 36.800 | 125 | 73 | 3 | 32 | 154 | 500 | 620 | 7.54e-13 | 70.9 |
Find 144 sgRNAs with CRISPR-Local
Find 609 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGGCTGCCTTGGCATA+GGG | 0.536458 | 3:-88983433 | MsG0380016883.01.T01:intron |
| AAACATACAGCTGCCATTTC+TGG | 0.213317 | 3:-88983216 | MsG0380016883.01.T01:intron |
| AAAGAATGATAAAATACAAC+AGG | 0.491533 | 3:+88980920 | None:intergenic |
| AAAGAGATTCGCTGCGTTAT+GGG | 0.389516 | 3:-88989901 | MsG0380016883.01.T01:CDS |
| AAAGCATTGCAAGTTTCATG+TGG | 0.626884 | 3:-88981163 | MsG0380016883.01.T01:CDS |
| AAAGGGATTGACAAATTGGT+AGG | 0.579916 | 3:-88983757 | MsG0380016883.01.T01:CDS |
| AAAGGTTCAAGGGCCATTCT+TGG | 0.349910 | 3:-88983271 | MsG0380016883.01.T01:CDS |
| AAAGTTGTACGGATCTCTGC+TGG | 0.539892 | 3:-88988633 | MsG0380016883.01.T01:CDS |
| AACCTCAGAACTTGTGCTTT+AGG | 0.301018 | 3:-88989963 | MsG0380016883.01.T01:CDS |
| AACGCAGCGAATCTCTTTCC+CGG | 0.419591 | 3:+88989906 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATGCATGAAAAATCCC+TGG | - | Chr3:88983776-88983795 | MsG0380016883.01.T01:CDS | 30.0% | |
| AAAAATGCTGTTGAGCATAT+TGG | - | Chr3:88985026-88985045 | MsG0380016883.01.T01:intron | 30.0% | |
| AAAATGCTGTTGAGCATATT+GGG | - | Chr3:88985027-88985046 | MsG0380016883.01.T01:intron | 30.0% | |
| AAACTTATGCTTTCAACTCA+GGG | - | Chr3:88989586-88989605 | MsG0380016883.01.T01:intron | 30.0% | |
| AACCATAGTCAAAATAGTTG+TGG | + | Chr3:88986147-88986166 | None:intergenic | 30.0% | |
| AACCTTATCTTTAATCAAGG+CGG | + | Chr3:88986261-88986280 | None:intergenic | 30.0% | |
| AAGACGAAAAGAAAAATTGG+TGG | + | Chr3:88984850-88984869 | None:intergenic | 30.0% | |
| AAGTAATCATACAACTGAGT+AGG | + | Chr3:88982007-88982026 | None:intergenic | 30.0% | |
| AATGAAATGGAAAATGGAAG+TGG | + | Chr3:88981113-88981132 | None:intergenic | 30.0% | |
| AATGATGCACATAATAGATG+AGG | + | Chr3:88983615-88983634 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 88980834 | 88990018 | 88980834 | ID=MsG0380016883.01;Name=MsG0380016883.01 |
| Chr3 | mRNA | 88980834 | 88990018 | 88980834 | ID=MsG0380016883.01.T01;Parent=MsG0380016883.01;Name=MsG0380016883.01.T01;_AED=0.38;_eAED=0.38;_QI=0|0.85|0.81|1|0.80|0.81|22|292|415 |
| Chr3 | exon | 88980834 | 88981245 | 88980834 | ID=MsG0380016883.01.T01:exon:5671;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88981615 | 88981694 | 88981615 | ID=MsG0380016883.01.T01:exon:5670;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88983217 | 88983320 | 88983217 | ID=MsG0380016883.01.T01:exon:5669;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88983434 | 88983488 | 88983434 | ID=MsG0380016883.01.T01:exon:5668;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88983749 | 88983808 | 88983749 | ID=MsG0380016883.01.T01:exon:5667;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88983928 | 88983973 | 88983928 | ID=MsG0380016883.01.T01:exon:5666;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88984086 | 88984128 | 88984086 | ID=MsG0380016883.01.T01:exon:5665;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88986244 | 88986337 | 88986244 | ID=MsG0380016883.01.T01:exon:5664;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88987706 | 88987885 | 88987706 | ID=MsG0380016883.01.T01:exon:5663;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88987999 | 88988058 | 88987999 | ID=MsG0380016883.01.T01:exon:5662;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88988607 | 88988685 | 88988607 | ID=MsG0380016883.01.T01:exon:5661;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88988838 | 88988914 | 88988838 | ID=MsG0380016883.01.T01:exon:5660;Parent=MsG0380016883.01.T01 |
| Chr3 | exon | 88989769 | 88990018 | 88989769 | ID=MsG0380016883.01.T01:exon:5659;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88989769 | 88990018 | 88989769 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88988838 | 88988914 | 88988838 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88988607 | 88988685 | 88988607 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88987999 | 88988058 | 88987999 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88987706 | 88987885 | 88987706 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88986244 | 88986337 | 88986244 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88984086 | 88984128 | 88984086 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88983928 | 88983973 | 88983928 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88983749 | 88983808 | 88983749 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88983434 | 88983488 | 88983434 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88983217 | 88983320 | 88983217 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88981615 | 88981694 | 88981615 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | CDS | 88981126 | 88981245 | 88981126 | ID=MsG0380016883.01.T01:cds;Parent=MsG0380016883.01.T01 |
| Chr3 | three_prime_UTR | 88980834 | 88981125 | 88980834 | ID=MsG0380016883.01.T01:three_prime_utr;Parent=MsG0380016883.01.T01 |
| Gene Sequence |
>MsG0380016883.01.T01 ATGTGGCACCGTTTATCATCATCATCCAAATTCAACCTCAGAACTTGTGCTTTAGGGTTAGGGTTTCTTAGACGATCATTCTCCTCTGAACCGGGAAAGAGATTCGCTGCGTTATGGGGCAACGGCGATTACGGTAGATTGGGTCTCGGTAATTTGAATTCTCAATGGAAACCTGCTATTTGCACCTCCTTCCATAATCAAAACGTTCAAGCAATTGCTTGTGGTGGTGCTCACACGCTATTCTTAACAGACAATGGATGTGTTTATGCTACTGGTCTCAATGATTTTGGACAGCTCGGTACATCGGAGAGTAAACCCTACTCAGTTGTGCCACTTCAAGTTTTTGGGCATGAGAAGAAAGTTGTACGGATCTCTGCTGGTTATAATCATTCCAGTGCAATAACAGTGGATGGAGAACTGTACATGTGGGGAAAGAATACAACTGGGCAGCTTGGACTTGGAAAAAAATCCCCTATAGCTGATAAGGTTGGCTGGTGGAAAGTGGAAACTACTATGAGTATAATGTCATTTTACTTACATGCAGAGGCTCCCAACATAGTTCCTTTGCCAACTAAAGTGGAATACTTGGATGGAATCACCATTGAAATGGCTGCTCTGGGTTCTGAGCACTCACTGGCTATTAGTGATGGAGGAGAGGCCTATAGTTGGGGAACAGGAGCGTCTGGTAGACTTGGTCATGGTCATGAGTCAAGCATACTAGGATTCTTCAAAAGTTACAGTGAATACAGCCCAAGGCTTATAAGGGATCTCGAAGGAATCAAGGTAAAATATGTAGCTGCTGGGATGCTGACCTCCGCTTGTACTGATGAAAATGGCTCTGTGTTTATATTTGGAGATAAAGGGATTGACAAATTGGTAGGTGCAATCGATTTTGGTGAACTTTATACTTGGGGTTCAAATGAAAATGGCTGCCTTGGCATAGGGTCCTCATATGCCATTCATCTACCTGAAAAGGTTCAAGGGCCATTCTTGGAGTCTTCTGTTGTTCAGGTTTCTTGTGGCTGGAAACATACAGCTGCCATTTCTGGGGGAAGAATCTTCACATGGGGTTGGGGAGGTTCAAATGGAACTTTTTCTGACGATGGACATTCTTCAGGTGGGCAATTGGGTCATGGAAGTGATGTGGATTATATAAACCCTTCAAGAGTTTGCTTTGCAGAAGACGTGAAAGCATTGCAAGTTTCATGTGGTTTCAATCATACAGGTGCTATATTTGAGTATGCATAG |
| Protein sequence |
>MsG0380016883.01.T01 MWHRLSSSSKFNLRTCALGLGFLRRSFSSEPGKRFAALWGNGDYGRLGLGNLNSQWKPAICTSFHNQNVQAIACGGAHTLFLTDNGCVYATGLNDFGQLGTSESKPYSVVPLQVFGHEKKVVRISAGYNHSSAITVDGELYMWGKNTTGQLGLGKKSPIADKVGWWKVETTMSIMSFYLHAEAPNIVPLPTKVEYLDGITIEMAALGSEHSLAISDGGEAYSWGTGASGRLGHGHESSILGFFKSYSEYSPRLIRDLEGIKVKYVAAGMLTSACTDENGSVFIFGDKGIDKLVGAIDFGELYTWGSNENGCLGIGSSYAIHLPEKVQGPFLESSVVQVSCGWKHTAAISGGRIFTWGWGGSNGTFSDDGHSSGGQLGHGSDVDYINPSRVCFAEDVKALQVSCGFNHTGAIFEYA* |
