AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017810.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017810.01.T01 MTR_3g112410 70.270 111 33 0 2 112 3 113 1.92e-56 171
MsG0380017810.01.T01 MTR_1g023140 69.091 110 34 0 2 111 3 112 1.18e-54 166
MsG0380017810.01.T01 MTR_5g021180 59.483 116 47 0 2 117 5 120 1.69e-50 156
MsG0380017810.01.T01 MTR_3g112400 67.647 102 13 3 1 102 1 82 5.14e-38 123
MsG0380017810.01.T01 MTR_7g009070 46.667 105 56 0 7 111 28 132 1.23e-31 108
MsG0380017810.01.T01 MTR_8g005320 40.385 104 62 0 5 108 26 129 1.53e-31 108
MsG0380017810.01.T01 MTR_8g006685 40.385 104 62 0 5 108 95 198 5.25e-31 109
MsG0380017810.01.T01 MTR_8g006560 40.385 104 62 0 5 108 95 198 5.25e-31 109
MsG0380017810.01.T01 MTR_7g073680 43.000 100 57 0 5 104 24 123 1.45e-30 106
MsG0380017810.01.T01 MTR_8g107460 41.818 110 64 0 3 112 252 361 1.22e-28 107
MsG0380017810.01.T01 MTR_2g010750 42.241 116 65 1 4 117 8 123 7.83e-28 99.0
MsG0380017810.01.T01 MTR_2g082590 43.119 109 62 0 3 111 8 116 6.06e-27 96.7
MsG0380017810.01.T01 MTR_5g037950 45.794 107 58 0 7 113 21 127 1.04e-26 96.3
MsG0380017810.01.T01 MTR_4g081380 40.351 114 68 0 3 116 8 121 2.54e-25 92.8
MsG0380017810.01.T01 MTR_5g037890 43.119 109 62 0 2 110 15 123 3.41e-25 92.4
MsG0380017810.01.T01 MTR_5g037930 40.952 105 62 0 7 111 22 126 1.33e-24 90.9
MsG0380017810.01.T01 MTR_2g082590 38.525 122 62 1 3 111 8 129 1.22e-23 88.6
MsG0380017810.01.T01 MTR_4g094690 35.238 105 66 1 8 110 62 166 1.02e-19 79.7
MsG0380017810.01.T01 MTR_7g080250 38.938 113 63 3 5 116 75 182 1.16e-18 77.0
MsG0380017810.01.T01 MTR_3g089065 37.000 100 58 3 14 111 76 172 1.17e-15 69.3
MsG0380017810.01.T01 MTR_2g010750 37.778 90 50 1 4 93 8 91 2.07e-15 66.6
MsG0380017810.01.T01 MTR_1g098660 35.526 76 48 1 25 99 76 151 4.01e-15 67.8
MsG0380017810.01.T01 MTR_1g026125 31.193 109 71 2 7 111 183 291 5.18e-13 63.9
MsG0380017810.01.T01 MTR_4g081380 38.571 70 43 0 3 72 8 77 1.71e-12 58.9
MsG0380017810.01.T01 MTR_3g435370 32.967 91 57 2 4 92 86 174 3.27e-12 61.2
MsG0380017810.01.T01 MTR_8g059015 33.708 89 57 2 25 111 87 175 3.65e-12 60.1
MsG0380017810.01.T01 MTR_2g079420 31.250 112 74 3 2 111 62 172 3.82e-12 60.1
MsG0380017810.01.T01 MTR_3g435370 32.967 91 57 2 4 92 86 174 4.30e-12 60.8
MsG0380017810.01.T01 MTR_7g102130 34.667 75 47 1 31 105 49 121 1.88e-11 57.4
MsG0380017810.01.T01 MTR_1g114290 35.821 67 42 1 26 91 85 151 2.27e-11 58.2
MsG0380017810.01.T01 MTR_6g092700 29.545 88 58 2 7 92 77 162 2.52e-11 58.5
MsG0380017810.01.T01 MTR_4g088905 29.245 106 71 1 5 110 3 104 8.57e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017810.01.T01 AT3G51030 66.364 110 37 0 2 111 3 112 4.40e-54 165
MsG0380017810.01.T01 AT5G42980 58.559 111 46 0 1 111 1 111 2.64e-46 145
MsG0380017810.01.T01 AT1G45145 56.250 112 49 0 1 112 1 112 7.72e-46 144
MsG0380017810.01.T01 AT1G19730 53.571 112 51 1 2 112 3 114 1.77e-39 128
MsG0380017810.01.T01 AT3G08710 44.231 104 58 0 5 108 23 126 2.73e-31 108
MsG0380017810.01.T01 AT3G08710 44.231 104 58 0 5 108 23 126 2.73e-31 108
MsG0380017810.01.T01 AT3G08710 44.231 104 58 0 5 108 23 126 2.73e-31 108
MsG0380017810.01.T01 AT5G39950 44.954 109 60 0 2 110 22 130 5.46e-30 104
MsG0380017810.01.T01 AT1G11530 43.363 113 64 0 5 117 2 114 3.64e-28 99.8
MsG0380017810.01.T01 AT3G17880 40.367 109 65 0 4 112 262 370 3.77e-28 105
MsG0380017810.01.T01 AT3G17880 40.367 109 65 0 4 112 269 377 4.75e-28 105
MsG0380017810.01.T01 AT3G56420 42.157 102 59 0 4 105 39 140 5.50e-28 100
MsG0380017810.01.T01 AT3G56420 42.157 102 59 0 4 105 39 140 5.50e-28 100
MsG0380017810.01.T01 AT3G56420 42.157 102 59 0 4 105 39 140 5.50e-28 100
MsG0380017810.01.T01 AT1G69880 39.623 106 64 0 7 112 39 144 2.31e-25 93.6
MsG0380017810.01.T01 AT2G40790 40.196 102 61 0 4 105 39 140 2.45e-25 93.6
MsG0380017810.01.T01 AT2G40790 40.196 102 61 0 4 105 39 140 2.45e-25 93.6
MsG0380017810.01.T01 AT1G59730 37.168 113 71 0 1 113 17 129 2.09e-24 90.5
MsG0380017810.01.T01 AT2G35010 39.806 103 60 1 4 104 83 185 1.92e-19 79.7
MsG0380017810.01.T01 AT2G35010 39.806 103 60 1 4 104 83 185 1.92e-19 79.7
MsG0380017810.01.T01 AT5G16400 35.849 106 64 2 5 109 78 180 5.41e-17 72.8
MsG0380017810.01.T01 AT1G31020 35.514 107 67 1 2 106 46 152 5.78e-17 72.4
MsG0380017810.01.T01 AT3G02730 36.792 106 63 2 5 109 68 170 1.12e-16 72.0
MsG0380017810.01.T01 AT1G43560 35.065 77 49 1 24 99 73 149 2.45e-15 68.2
MsG0380017810.01.T01 AT1G76760 34.211 76 49 1 25 99 79 154 6.25e-15 67.4
MsG0380017810.01.T01 AT1G07700 33.663 101 59 2 20 112 100 200 9.28e-13 62.0
MsG0380017810.01.T01 AT1G07700 33.663 101 59 2 20 112 100 200 9.28e-13 62.0
MsG0380017810.01.T01 AT1G07700 33.663 101 59 2 20 112 100 200 9.28e-13 62.0
MsG0380017810.01.T01 AT1G07700 33.663 101 59 2 20 112 113 213 1.72e-12 61.6
MsG0380017810.01.T01 AT4G26160 30.488 82 55 1 7 88 95 174 5.96e-12 60.5
MsG0380017810.01.T01 AT4G03520 29.245 106 70 3 8 111 82 184 6.64e-12 59.7
MsG0380017810.01.T01 AT1G76080 29.091 110 74 2 7 112 187 296 8.78e-11 57.8

Find 31 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGAGTCATTTCCCTTC+TGG 0.238843 3:+100882303 None:intergenic
TAGTTGATCTTACTGCTTCT+TGG 0.265306 3:-100882105 MsG0380017810.01.T01:CDS
TAATAAGACTGAGCTGAATT+CGG 0.378699 3:-100881760 MsG0380017810.01.T01:CDS
ATTCATCCACGTCAACCTTA+AGG 0.389106 3:+100882014 None:intergenic
AATAAGACTGAGCTGAATTC+GGG 0.398840 3:-100881759 MsG0380017810.01.T01:CDS
GCCAGATGTCACCTTCCTTA+AGG 0.403432 3:-100882029 MsG0380017810.01.T01:CDS
GATTTAACCAACCAGTTTGT+TGG 0.404325 3:+100882286 None:intergenic
GCTTTCCTTCTTTCAAGAAC+AGG 0.406276 3:+100881805 None:intergenic
GGGAAATGACTCCAACAAAC+TGG 0.461512 3:-100882297 MsG0380017810.01.T01:intron
ACATTCCTGTTCTTGAAAGA+AGG 0.465257 3:-100881810 MsG0380017810.01.T01:CDS
ACCTTAAGGAAGGTGACATC+TGG 0.488781 3:+100882028 None:intergenic
AAATCCACAGTGTTGAGGCT+TGG 0.494177 3:-100882337 MsG0380017810.01.T01:CDS
TTCAGACTGTTGCTGAGGAG+TGG 0.502035 3:-100881851 MsG0380017810.01.T01:intron
TCAGACTGTTGCTGAGGAGT+GGG 0.502412 3:-100881850 MsG0380017810.01.T01:intron
GATCTTACTGCTTCTTGGTG+TGG 0.514931 3:-100882100 MsG0380017810.01.T01:CDS
AAAGCTAGTGAACAAGGTTG+TGG 0.516587 3:-100881787 MsG0380017810.01.T01:CDS
AAGCTAGTGAACAAGGTTGT+GGG 0.524835 3:-100881786 MsG0380017810.01.T01:CDS
CACCTTCCTTAAGGTTGACG+TGG 0.565554 3:-100882020 MsG0380017810.01.T01:CDS
AGAAGGAAAGCTAGTGAACA+AGG 0.575053 3:-100881793 MsG0380017810.01.T01:CDS
ATTTCTTCAGACTGTTGCTG+AGG 0.578897 3:-100881856 MsG0380017810.01.T01:intron
GATTGAAATCCACAGTGTTG+AGG 0.593831 3:-100882342 MsG0380017810.01.T01:CDS
GAGTAGATAAGTACTCCGTC+CGG 0.599346 3:-100881737 MsG0380017810.01.T01:CDS
TTGGAACGAACAAATCCAGA+AGG 0.609233 3:-100882318 MsG0380017810.01.T01:CDS
TCGCAGAGTTGGCTCAAAAG+CGG 0.612847 3:-100882051 MsG0380017810.01.T01:CDS
AATGGGGCAATGAAACGGCA+TGG 0.612943 3:+100882076 None:intergenic
TCTTTCAAGAACAGGAATGT+CGG 0.617427 3:+100881813 None:intergenic
CAAGAACAGGAATGTCGGCA+TGG 0.627664 3:+100881818 None:intergenic
ATCCACGTCAACCTTAAGGA+AGG 0.640907 3:+100882018 None:intergenic
CTAGTGAACAAGGTTGTGGG+TGG 0.677639 3:-100881783 MsG0380017810.01.T01:CDS
CGTTCCAAGCCTCAACACTG+TGG 0.688562 3:+100882333 None:intergenic
TGGAACGAACAAATCCAGAA+GGG 0.692449 3:-100882317 MsG0380017810.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAGGGTAATTAATTAA+AGG + Chr3:100882215-100882234 None:intergenic 15.0%
!! TCTTAAACACATACAAAATA+TGG + Chr3:100881885-100881904 None:intergenic 20.0%
! AAGACTGTTATTGAAGATTA+TGG - Chr3:100881901-100881920 MsG0380017810.01.T01:intron 25.0%
! AAGGTCAAAGATTTAACATA+AGG + Chr3:100881844-100881863 None:intergenic 25.0%
! CAAAGATTTAACATAAGGAT+AGG + Chr3:100881839-100881858 None:intergenic 25.0%
! GCAGTTAAAAATTCTAACAA+AGG + Chr3:100881863-100881882 None:intergenic 25.0%
GTTCTTCTAGATTAATCTTC+AGG + Chr3:100882170-100882189 None:intergenic 30.0%
TAAGGATAGGAAATATGAGA+AGG + Chr3:100881826-100881845 None:intergenic 30.0%
TAATAAGACTGAGCTGAATT+CGG - Chr3:100882327-100882346 MsG0380017810.01.T01:CDS 30.0%
TACGTGACATGACATTATTA+TGG - Chr3:100882129-100882148 MsG0380017810.01.T01:intron 30.0%
AATAAGACTGAGCTGAATTC+GGG - Chr3:100882328-100882347 MsG0380017810.01.T01:CDS 35.0%
ACATTCCTGTTCTTGAAAGA+AGG - Chr3:100882277-100882296 MsG0380017810.01.T01:intron 35.0%
AGCAACAGTCTGAAGAAATT+AGG + Chr3:100882230-100882249 None:intergenic 35.0%
ATTGAAGATTATGGCATCGT+TGG - Chr3:100881910-100881929 MsG0380017810.01.T01:intron 35.0%
GATTTAACCAACCAGTTTGT+TGG + Chr3:100881804-100881823 None:intergenic 35.0%
GCAACAGTCTGAAGAAATTA+GGG + Chr3:100882229-100882248 None:intergenic 35.0%
TAGTTGATCTTACTGCTTCT+TGG - Chr3:100881982-100882001 MsG0380017810.01.T01:intron 35.0%
TCTTTCAAGAACAGGAATGT+CGG + Chr3:100882277-100882296 None:intergenic 35.0%
AAAGCTAGTGAACAAGGTTG+TGG - Chr3:100882300-100882319 MsG0380017810.01.T01:CDS 40.0%
AAGCTAGTGAACAAGGTTGT+GGG - Chr3:100882301-100882320 MsG0380017810.01.T01:CDS 40.0%
AATGACTCCAACAAACTGGT+TGG - Chr3:100881794-100881813 MsG0380017810.01.T01:CDS 40.0%
AGAAGGAAAGCTAGTGAACA+AGG - Chr3:100882294-100882313 MsG0380017810.01.T01:intron 40.0%
ATTCATCCACGTCAACCTTA+AGG + Chr3:100882076-100882095 None:intergenic 40.0%
ATTTCTTCAGACTGTTGCTG+AGG - Chr3:100882231-100882250 MsG0380017810.01.T01:intron 40.0%
CGAAAAATGGGGCAATGAAA+CGG + Chr3:100882019-100882038 None:intergenic 40.0%
GATTGAAATCCACAGTGTTG+AGG - Chr3:100881745-100881764 MsG0380017810.01.T01:CDS 40.0%
GCTTTCCTTCTTTCAAGAAC+AGG + Chr3:100882285-100882304 None:intergenic 40.0%
TGGAACGAACAAATCCAGAA+GGG - Chr3:100881770-100881789 MsG0380017810.01.T01:CDS 40.0%
TTGGAACGAACAAATCCAGA+AGG - Chr3:100881769-100881788 MsG0380017810.01.T01:CDS 40.0%
ACCTTAAGGAAGGTGACATC+TGG + Chr3:100882062-100882081 None:intergenic 45.0%
AGCCAACTCTGCGAAAAATG+GGG + Chr3:100882030-100882049 None:intergenic 45.0%
ATCCACGTCAACCTTAAGGA+AGG + Chr3:100882072-100882091 None:intergenic 45.0%
GAGCCAACTCTGCGAAAAAT+GGG + Chr3:100882031-100882050 None:intergenic 45.0%
GAGTAGATAAGTACTCCGTC+CGG - Chr3:100882350-100882369 MsG0380017810.01.T01:CDS 45.0%
GATCTTACTGCTTCTTGGTG+TGG - Chr3:100881987-100882006 MsG0380017810.01.T01:intron 45.0%
GGGAAATGACTCCAACAAAC+TGG - Chr3:100881790-100881809 MsG0380017810.01.T01:CDS 45.0%
TGAGCCAACTCTGCGAAAAA+TGG + Chr3:100882032-100882051 None:intergenic 45.0%
TGTTGGAGTCATTTCCCTTC+TGG + Chr3:100881787-100881806 None:intergenic 45.0%
! AAATCCACAGTGTTGAGGCT+TGG - Chr3:100881750-100881769 MsG0380017810.01.T01:CDS 45.0%
AATGGGGCAATGAAACGGCA+TGG + Chr3:100882014-100882033 None:intergenic 50.0%
CAAGAACAGGAATGTCGGCA+TGG + Chr3:100882272-100882291 None:intergenic 50.0%
CACCTTCCTTAAGGTTGACG+TGG - Chr3:100882067-100882086 MsG0380017810.01.T01:CDS 50.0%
CTAGTGAACAAGGTTGTGGG+TGG - Chr3:100882304-100882323 MsG0380017810.01.T01:CDS 50.0%
GCCAGATGTCACCTTCCTTA+AGG - Chr3:100882058-100882077 MsG0380017810.01.T01:CDS 50.0%
TCAGACTGTTGCTGAGGAGT+GGG - Chr3:100882237-100882256 MsG0380017810.01.T01:intron 50.0%
TTCAGACTGTTGCTGAGGAG+TGG - Chr3:100882236-100882255 MsG0380017810.01.T01:intron 50.0%
! TCGCAGAGTTGGCTCAAAAG+CGG - Chr3:100882036-100882055 MsG0380017810.01.T01:CDS 50.0%
!! TGCCCCATTTTTCGCAGAGT+TGG - Chr3:100882025-100882044 MsG0380017810.01.T01:CDS 50.0%
CGTTCCAAGCCTCAACACTG+TGG + Chr3:100881757-100881776 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 100881725 100882384 100881725 ID=MsG0380017810.01;Name=MsG0380017810.01
Chr3 mRNA 100881725 100882384 100881725 ID=MsG0380017810.01.T01;Parent=MsG0380017810.01;Name=MsG0380017810.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|3|0|117
Chr3 exon 100882298 100882384 100882298 ID=MsG0380017810.01.T01:exon:13087;Parent=MsG0380017810.01.T01
Chr3 exon 100882009 100882131 100882009 ID=MsG0380017810.01.T01:exon:13086;Parent=MsG0380017810.01.T01
Chr3 exon 100881725 100881868 100881725 ID=MsG0380017810.01.T01:exon:13085;Parent=MsG0380017810.01.T01
Chr3 CDS 100882298 100882384 100882298 ID=MsG0380017810.01.T01:cds;Parent=MsG0380017810.01.T01
Chr3 CDS 100882009 100882131 100882009 ID=MsG0380017810.01.T01:cds;Parent=MsG0380017810.01.T01
Chr3 CDS 100881725 100881868 100881725 ID=MsG0380017810.01.T01:cds;Parent=MsG0380017810.01.T01
Gene Sequence

>MsG0380017810.01.T01

ATGGCGGAAGAAGGAAAAGTGATTGAAATCCACAGTGTTGAGGCTTGGAACGAACAAATCCAGAAGGGAAATGACTCCAACAAACTGATTGTAGTTGATCTTACTGCTTCTTGGTGTGGTCCATGCCGTTTCATTGCCCCATTTTTCGCAGAGTTGGCTCAAAAGCGGCCAGATGTCACCTTCCTTAAGGTTGACGTGGATGAATTGAATACTGTTGCTGAGGAGTGGGAAATTGAAGCCATGCCGACATTCCTGTTCTTGAAAGAAGGAAAGCTAGTGAACAAGGTTGTGGGTGGTAATAAGACTGAGCTGAATTCGGGAGTAGATAAGTACTCCGTCCGGTCTCACATATAA

Protein sequence

>MsG0380017810.01.T01

MAEEGKVIEIHSVEAWNEQIQKGNDSNKLIVVDLTASWCGPCRFIAPFFAELAQKRPDVTFLKVDVDELNTVAEEWEIEAMPTFLFLKEGKLVNKVVGGNKTELNSGVDKYSVRSHI*