AlfalfaGEDB | MsG0480022086.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022086.01

KEGG: -
GO: -
PFAM: PF07802

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022086.01.T01	KEH30790.1	93.359	256	15	1	1	256	1	254	1.34E-142	410

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002738.01	MsG0480022086.01	0.834917	2.270649e-56	2.733653e-53
MsG0180003585.01	MsG0480022086.01	0.837593	4.744769e-57	6.193001e-54
MsG0180004868.01	MsG0480022086.01	0.821250	4.462762e-53	3.611611e-50
MsG0180005789.01	MsG0480022086.01	0.821510	3.886250e-53	3.167906e-50
MsG0280008736.01	MsG0480022086.01	0.825772	3.906157e-54	3.594569e-51
MsG0280010792.01	MsG0480022086.01	0.801976	6.982240e-49	3.380064e-46
MsG0380014507.01	MsG0480022086.01	0.807514	4.870214e-50	2.718066e-47
MsG0480021417.01	MsG0480022086.01	0.812311	4.519740e-51	2.863942e-48
MsG0480022086.01	MsG0580024738.01	0.803110	4.075304e-49	2.030701e-46
MsG0480022086.01	MsG0580029047.01	0.836902	7.127205e-57	9.108147e-54

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022086.01.T01	MTR_4g082300	93.359	256	15	1	1	256	1	254	1.63e-146	410
MsG0480022086.01.T01	MTR_4g082300	92.969	256	16	1	1	256	1	254	1.29e-144	409
MsG0480022086.01.T01	MTR_4g082300	49.115	226	69	4	24	249	162	341	1.63e-59	192
MsG0480022086.01.T01	MTR_4g082300	70.130	77	23	0	49	125	275	351	4.66e-32	120

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022086.01.T01	AT1G14060	72.881	59	16	0	47	105	82	140	3.39e-28	106
MsG0480022086.01.T01	AT1G14060	46.667	90	48	0	150	239	51	140	1.61e-19	83.6
MsG0480022086.01.T01	AT5G02210	47.191	89	44	1	34	119	65	153	1.40e-18	80.5
MsG0480022086.01.T01	AT5G02210	40.000	75	42	1	177	248	74	148	1.70e-11	61.6
MsG0480022086.01.T01	AT5G57570	54.545	66	29	1	47	111	53	118	1.34e-14	69.3
MsG0480022086.01.T01	AT5G57640	45.333	75	41	0	45	119	82	156	8.05e-14	69.3

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Find 87 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAGCAACCAGCTCCGG+AGG	0.645801	4:+68046043	MsG0480022086.01.T01:CDS
AACCGCCTCCTTTCATGAAT+AGG	0.411096	4:-68044689	None:intergenic
AACCGTCTCCTTTAATGAAC+AGG	0.424322	4:-68046112	None:intergenic
ACAATTCTCCCATTCAACCA+AGG	0.704048	4:-68046144	None:intergenic
ACATTTGATTAGAATGAGAA+GGG	0.532264	4:-68046307	None:intergenic
ACCAGCTCCGGAGGCTACGA+AGG	0.553191	4:+68046052	MsG0480022086.01.T01:CDS
ACCTCGCTGAAAAGTGCTCT+GGG	0.429532	4:+68046195	MsG0480022086.01.T01:CDS
ACTCGTTTGTTGCAGTGTAT+GGG	0.481333	4:+68046224	MsG0480022086.01.T01:CDS
AGAAACCGCCGCCGAAGGCG+AGG	0.522261	4:+68044635	MsG0480022086.01.T01:CDS
AGACGGTTGCAGAGACACCT+TGG	0.584640	4:+68046127	MsG0480022086.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCATCCTTTACAATGCATA+TGG	+	Chr4:68045311-68045330	MsG0480022086.01.T01:intron	30.0%
ATTCTTACACAACAGCAAAT+AGG	-	Chr4:68045293-68045312	None:intergenic	30.0%
CACATTTGATTAGAATGAGA+AGG	-	Chr4:68046311-68046330	None:intergenic	30.0%
CATAATATATATGCAAGGTC+TGG	+	Chr4:68045710-68045729	MsG0480022086.01.T01:intron	30.0%
CATTTGATTAGAATGAGAAG+GGG	-	Chr4:68046309-68046328	None:intergenic	30.0%
GGTTGAAGTATGTGATTAAA+TGG	+	Chr4:68045423-68045442	MsG0480022086.01.T01:intron	30.0%
TAATATATATGCAAGGTCTG+GGG	+	Chr4:68045712-68045731	MsG0480022086.01.T01:intron	30.0%
TCAAAAGTCAACAAACTAAG+TGG	-	Chr4:68045360-68045379	None:intergenic	30.0%
TTCAGTAATTAGTGATTCAG+TGG	+	Chr4:68045550-68045569	MsG0480022086.01.T01:intron	30.0%
ACTTCTGGTATAGAATGAGA+AGG	-	Chr4:68045795-68045814	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	68044540	68046331	68044540	ID=MsG0480022086.01;Name=MsG0480022086.01
Chr4	mRNA	68044540	68046331	68044540	ID=MsG0480022086.01.T01;Parent=MsG0480022086.01;Name=MsG0480022086.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|0\|0\|1\|1\|1\|2\|0\|256
Chr4	exon	68044540	68044970	68044540	ID=MsG0480022086.01.T01:exon:18409;Parent=MsG0480022086.01.T01
Chr4	exon	68045992	68046331	68045992	ID=MsG0480022086.01.T01:exon:18410;Parent=MsG0480022086.01.T01
Chr4	CDS	68044540	68044970	68044540	ID=MsG0480022086.01.T01:cds;Parent=MsG0480022086.01.T01
Chr4	CDS	68045992	68046331	68045992	ID=MsG0480022086.01.T01:cds;Parent=MsG0480022086.01.T01

Gene Sequence

>MsG0480022086.01.T01

ATGTCGACGCCGCCGAACCCATTGGAAAACCCCAAATCCGATGCCAAACCATCAGATCCTCTTCCCAATCAACCACCACCGGAGTCTCCCGAACCAGAAACCGCCGCCGAAGGCGAGGAAGATGAAGAAGAAGGAGAATGCGGATTCTGCCTATTCATGAAAGGAGGCGGTTGCAAAGACACGTTCATCGCTTGGGAAGATTGCGTGAAAGAAGCTGAAGACAAAAATGAAGATCTTGTTGAAAAGTGCGCTGAGGTTACTGCAAAATTGAAGCAGTGTATGGATGAACATTCGGATTATTATGAACCCATTCTTAGGGCGGAGAAGCATGCTGAAGAGCAGGTTACCATTGAATTGGAGAAAGAAAAGCAGGATTTGGCCGCCGCCGCCGCCACCGCCGAGAATCAACAAGAATCCCCTTCTAATCAAAATGACCGTGAGTTGGAGTCAACGGTAATTTCAACGCCCTCAAAGCAAGCTGAAAAGGAGCAACCAGCTCCGGAGGCTACGAAGGCCGAAAACAAAGAGGAGGAGGGGGATTGCGGGTTCTGCCTGTTCATTAAAGGAGACGGTTGCAGAGACACCTTGGTTGAATGGGAGAATTGTGTGAAAGAGGCGAAGGAGAAGAAAGAGGACCTCGCTGAAAAGTGCTCTGGGGTCACCACTCGTTTGTTGCAGTGTATGGGTTCCAGTTCCGATTACTACAAACCCATCATCAAGACCGAGAAGCGCGTCGAAGAGCAAGCCCCTTCTCATTCTAATCAAATGTGA

Protein sequence

>MsG0480022086.01.T01

MSTPPNPLENPKSDAKPSDPLPNQPPPESPEPETAAEGEEDEEEGECGFCLFMKGGGCKDTFIAWEDCVKEAEDKNEDLVEKCAEVTAKLKQCMDEHSDYYEPILRAEKHAEEQVTIELEKEKQDLAAAAATAENQQESPSNQNDRELESTVISTPSKQAEKEQPAPEATKAENKEEEGDCGFCLFIKGDGCRDTLVEWENCVKEAKEKKEDLAEKCSGVTTRLLQCMGSSSDYYKPIIKTEKRVEEQAPSHSNQM*