AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022086.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022086.01.T01 MTR_4g082300 93.359 256 15 1 1 256 1 254 1.63e-146 410
MsG0480022086.01.T01 MTR_4g082300 92.969 256 16 1 1 256 1 254 1.29e-144 409
MsG0480022086.01.T01 MTR_4g082300 49.115 226 69 4 24 249 162 341 1.63e-59 192
MsG0480022086.01.T01 MTR_4g082300 70.130 77 23 0 49 125 275 351 4.66e-32 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022086.01.T01 AT1G14060 72.881 59 16 0 47 105 82 140 3.39e-28 106
MsG0480022086.01.T01 AT1G14060 46.667 90 48 0 150 239 51 140 1.61e-19 83.6
MsG0480022086.01.T01 AT5G02210 47.191 89 44 1 34 119 65 153 1.40e-18 80.5
MsG0480022086.01.T01 AT5G02210 40.000 75 42 1 177 248 74 148 1.70e-11 61.6
MsG0480022086.01.T01 AT5G57570 54.545 66 29 1 47 111 53 118 1.34e-14 69.3
MsG0480022086.01.T01 AT5G57640 45.333 75 41 0 45 119 82 156 8.05e-14 69.3

Find 87 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCCTTCGGCGGCGGTTTC+TGG 0.056859 4:-68044633 None:intergenic
ATTATTATGAACCCATTCTT+AGG 0.179261 4:+68044835 MsG0480022086.01.T01:CDS
CACTCGTTTGTTGCAGTGTA+TGG 0.222714 4:+68046223 MsG0480022086.01.T01:CDS
TGATGGTTTGGCATCGGATT+TGG 0.227434 4:-68044571 None:intergenic
GATGGTTTGGCATCGGATTT+GGG 0.232683 4:-68044570 None:intergenic
GGGTTCTGCCTGTTCATTAA+AGG 0.259132 4:+68046104 MsG0480022086.01.T01:CDS
CTCGGCGGTGGCGGCGGCGG+CGG 0.273906 4:-68044919 None:intergenic
TCGGCGGCGGTTTCTGGTTC+GGG 0.299995 4:-68044627 None:intergenic
TTCGGCGGCGGTTTCTGGTT+CGG 0.312231 4:-68044628 None:intergenic
CGCTTCTCGGTCTTGATGAT+GGG 0.346732 4:-68046269 None:intergenic
GACTCCGGTGGTGGTTGATT+GGG 0.354635 4:-68044603 None:intergenic
GCGCTTCTCGGTCTTGATGA+TGG 0.357410 4:-68046270 None:intergenic
TTGCTCTTCGACGCGCTTCT+CGG 0.361977 4:-68046282 None:intergenic
GGAGAAAGAAAAGCAGGATT+TGG 0.362471 4:+68044896 MsG0480022086.01.T01:CDS
TTCTTCATCTTCCTCGCCTT+CGG 0.365927 4:-68044646 None:intergenic
GATGATGGGTTTGTAGTAAT+CGG 0.384814 4:-68046255 None:intergenic
AGACTCCGGTGGTGGTTGAT+TGG 0.385111 4:-68044604 None:intergenic
AGGGGATTCTTGTTGATTCT+CGG 0.385534 4:-68044937 None:intergenic
AGAGCAGGTTACCATTGAAT+TGG 0.397436 4:+68044875 MsG0480022086.01.T01:CDS
ATTCTCGGCGGTGGCGGCGG+CGG 0.403836 4:-68044922 None:intergenic
AACCGCCTCCTTTCATGAAT+AGG 0.411096 4:-68044689 None:intergenic
ATGCTTCTCCGCCCTAAGAA+TGG 0.411420 4:-68044847 None:intergenic
GCAGAGACACCTTGGTTGAA+TGG 0.415028 4:+68046135 MsG0480022086.01.T01:CDS
ATCTTCCTCGCCTTCGGCGG+CGG 0.416651 4:-68044640 None:intergenic
AACCGTCTCCTTTAATGAAC+AGG 0.424322 4:-68046112 None:intergenic
ACCTCGCTGAAAAGTGCTCT+GGG 0.429532 4:+68046195 MsG0480022086.01.T01:CDS
GCAAAGACACGTTCATCGCT+TGG 0.430395 4:+68044712 MsG0480022086.01.T01:CDS
GACCTCGCTGAAAAGTGCTC+TGG 0.431197 4:+68046194 MsG0480022086.01.T01:CDS
GGGAAGAGGATCTGATGGTT+TGG 0.434881 4:-68044583 None:intergenic
GCCTTCGTAGCCTCCGGAGC+TGG 0.437486 4:-68046053 None:intergenic
CAGAGACACCTTGGTTGAAT+GGG 0.446480 4:+68046136 MsG0480022086.01.T01:CDS
TGCTTCTCCGCCCTAAGAAT+GGG 0.453023 4:-68044846 None:intergenic
CAAAGACACGTTCATCGCTT+GGG 0.455392 4:+68044713 MsG0480022086.01.T01:CDS
TTATTATGAACCCATTCTTA+GGG 0.456966 4:+68044836 MsG0480022086.01.T01:CDS
TTGATTCTCGGCGGTGGCGG+CGG 0.458622 4:-68044925 None:intergenic
GCCCTCAAAGCAAGCTGAAA+AGG 0.468090 4:+68046025 MsG0480022086.01.T01:CDS
GGGTTTGTAGTAATCGGAAC+TGG 0.476444 4:-68046249 None:intergenic
TGCAAAATTGAAGCAGTGTA+TGG 0.476710 4:+68044800 MsG0480022086.01.T01:CDS
ACTCGTTTGTTGCAGTGTAT+GGG 0.481333 4:+68046224 MsG0480022086.01.T01:CDS
GGCCGAAAACAAAGAGGAGG+AGG 0.485890 4:+68046073 MsG0480022086.01.T01:CDS
TGAAAAGGAGCAACCAGCTC+CGG 0.488322 4:+68046040 MsG0480022086.01.T01:CDS
GCAGTGTATGGATGAACATT+CGG 0.489367 4:+68044812 MsG0480022086.01.T01:CDS
GGTTCGGGAGACTCCGGTGG+TGG 0.501484 4:-68044612 None:intergenic
CACATTTGATTAGAATGAGA+AGG 0.505801 4:-68046308 None:intergenic
TGAATTGGAGAAAGAAAAGC+AGG 0.511809 4:+68044890 MsG0480022086.01.T01:CDS
GTTTCTGGTTCGGGAGACTC+CGG 0.516638 4:-68044618 None:intergenic
AGAAACCGCCGCCGAAGGCG+AGG 0.522261 4:+68044635 MsG0480022086.01.T01:CDS
GAACCAGAAACCGCCGCCGA+AGG 0.523723 4:+68044630 MsG0480022086.01.T01:CDS
TTGTTGATTCTCGGCGGTGG+CGG 0.528626 4:-68044928 None:intergenic
CCGAAAACAAAGAGGAGGAG+GGG 0.530892 4:+68046075 MsG0480022086.01.T01:CDS
TGCCTGTTCATTAAAGGAGA+CGG 0.531952 4:+68046110 MsG0480022086.01.T01:CDS
ACATTTGATTAGAATGAGAA+GGG 0.532264 4:-68046307 None:intergenic
GGCGAGGAAGATGAAGAAGA+AGG 0.541499 4:+68044651 MsG0480022086.01.T01:CDS
GCCGAAAACAAAGAGGAGGA+GGG 0.552949 4:+68046074 MsG0480022086.01.T01:CDS
ACCAGCTCCGGAGGCTACGA+AGG 0.553191 4:+68046052 MsG0480022086.01.T01:CDS
AGGATCTGATGGTTTGGCAT+CGG 0.555085 4:-68044577 None:intergenic
TGCCTATTCATGAAAGGAGG+CGG 0.555309 4:+68044687 MsG0480022086.01.T01:CDS
GTCGACGCCGCCGAACCCAT+TGG 0.556805 4:+68044542 MsG0480022086.01.T01:CDS
TTCTTGTTGATTCTCGGCGG+TGG 0.557772 4:-68044931 None:intergenic
TGATTGGGAAGAGGATCTGA+TGG 0.558958 4:-68044588 None:intergenic
CGAAAACAAAGAGGAGGAGG+GGG 0.568503 4:+68046076 MsG0480022086.01.T01:CDS
GAATTGTGTGAAAGAGGCGA+AGG 0.570129 4:+68046160 MsG0480022086.01.T01:CDS
GGAGAAGCATGCTGAAGAGC+AGG 0.570146 4:+68044860 MsG0480022086.01.T01:CDS
GGTGGTGGTTGATTGGGAAG+AGG 0.571966 4:-68044597 None:intergenic
TCTGGTTCGGGAGACTCCGG+TGG 0.575115 4:-68044615 None:intergenic
AGAGGCGAAGGAGAAGAAAG+AGG 0.580829 4:+68046172 MsG0480022086.01.T01:CDS
TCTTCCCAATCAACCACCAC+CGG 0.583567 4:+68044599 MsG0480022086.01.T01:CDS
AGACGGTTGCAGAGACACCT+TGG 0.584640 4:+68046127 MsG0480022086.01.T01:CDS
GAACCGCAGTGACCGTGAGT+TGG 0.587819 4:+68045983 MsG0480022086.01.T01:intron
TTCATCTTCCTCGCCTTCGG+CGG 0.591064 4:-68044643 None:intergenic
TGACCGTGAGTTGGAGTCAA+CGG 0.592923 4:+68045992 MsG0480022086.01.T01:CDS
GATGAAGAAGAAGGAGAATG+CGG 0.600982 4:+68044660 MsG0480022086.01.T01:CDS
ATGGGAGAATTGTGTGAAAG+AGG 0.614008 4:+68046154 MsG0480022086.01.T01:CDS
ATGGTTTGGCATCGGATTTG+GGG 0.630819 4:-68044569 None:intergenic
GGATTCTGCCTATTCATGAA+AGG 0.631082 4:+68044681 MsG0480022086.01.T01:CDS
CATTTGATTAGAATGAGAAG+GGG 0.632779 4:-68046306 None:intergenic
TTCTGCCTATTCATGAAAGG+AGG 0.642527 4:+68044684 MsG0480022086.01.T01:CDS
AAAGGAGCAACCAGCTCCGG+AGG 0.645801 4:+68046043 MsG0480022086.01.T01:CDS
TTATGAACCCATTCTTAGGG+CGG 0.646080 4:+68044839 MsG0480022086.01.T01:CDS
GAAGGCCGAAAACAAAGAGG+AGG 0.662357 4:+68046070 MsG0480022086.01.T01:CDS
TACGAAGGCCGAAAACAAAG+AGG 0.665700 4:+68046067 MsG0480022086.01.T01:CDS
TTACCGTTGACTCCAACTCA+CGG 0.671368 4:-68045995 None:intergenic
GGATTCTTGTTGATTCTCGG+CGG 0.677920 4:-68044934 None:intergenic
CATACACTGCAACAAACGAG+TGG 0.693657 4:-68046222 None:intergenic
ACAATTCTCCCATTCAACCA+AGG 0.704048 4:-68046144 None:intergenic
TCTTGTTGAAAAGTGCGCTG+AGG 0.705355 4:+68044773 MsG0480022086.01.T01:CDS
CCTCGCTGAAAAGTGCTCTG+GGG 0.729378 4:+68046196 MsG0480022086.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTCAATTGATAAAATT+AGG + Chr4:68045061-68045080 MsG0480022086.01.T01:intron 10.0%
!!! TTGTTTTTTAATTTTTGATA+TGG + Chr4:68045209-68045228 MsG0480022086.01.T01:intron 10.0%
!!! ATTTTATCAATTGAAATATG+TGG - Chr4:68045060-68045079 None:intergenic 15.0%
!!! TCTATTTAATTTGTTGATTT+GGG + Chr4:68045487-68045506 MsG0480022086.01.T01:intron 15.0%
!!! TTCTATTTAATTTGTTGATT+TGG + Chr4:68045486-68045505 MsG0480022086.01.T01:intron 15.0%
!!! TTTTATCAATTGAAATATGT+GGG - Chr4:68045059-68045078 None:intergenic 15.0%
!! TAAGTGGAATTATTATTGAT+TGG + Chr4:68045159-68045178 MsG0480022086.01.T01:intron 20.0%
!! TTTGAGAATCTTTAATAATG+AGG + Chr4:68045375-68045394 MsG0480022086.01.T01:intron 20.0%
!!! AAAAATCACTTTTGATTAGA+AGG - Chr4:68044960-68044979 None:intergenic 20.0%
!!! AAAATCACTTTTGATTAGAA+GGG - Chr4:68044959-68044978 None:intergenic 20.0%
! ACATTTGATTAGAATGAGAA+GGG - Chr4:68046310-68046329 None:intergenic 25.0%
! AGAGTAGAGATTCTAATTAA+TGG + Chr4:68045611-68045630 MsG0480022086.01.T01:intron 25.0%
! ATAATATATATGCAAGGTCT+GGG + Chr4:68045711-68045730 MsG0480022086.01.T01:intron 25.0%
! ATTATTATGAACCCATTCTT+AGG + Chr4:68044835-68044854 MsG0480022086.01.T01:CDS 25.0%
! CAATGCATAATATATATGCA+AGG + Chr4:68045705-68045724 MsG0480022086.01.T01:intron 25.0%
! TTATTATGAACCCATTCTTA+GGG + Chr4:68044836-68044855 MsG0480022086.01.T01:CDS 25.0%
! TTGATATGGTTATCTATAAG+TGG + Chr4:68045223-68045242 MsG0480022086.01.T01:intron 25.0%
!! ATAGATATGTGAATCTCTTT+TGG + Chr4:68045401-68045420 MsG0480022086.01.T01:intron 25.0%
!! TAGATATGTGAATCTCTTTT+GGG + Chr4:68045402-68045421 MsG0480022086.01.T01:intron 25.0%
!! TCAATTTTTGTGACAATAAG+TGG + Chr4:68045143-68045162 MsG0480022086.01.T01:intron 25.0%
!!! AAATCACTTTTGATTAGAAG+GGG - Chr4:68044958-68044977 None:intergenic 25.0%
!!! CTATTTTTTTTTTTTGGTGG+TGG - Chr4:68045750-68045769 None:intergenic 25.0%
!!! GGTAATTGTATTTTGATTGA+GGG + Chr4:68045571-68045590 MsG0480022086.01.T01:intron 25.0%
!!! GTAGCGCTATTTTTTTTTTT+TGG - Chr4:68045756-68045775 None:intergenic 25.0%
!!! TGGTAATTGTATTTTGATTG+AGG + Chr4:68045570-68045589 MsG0480022086.01.T01:intron 25.0%
ATCATCCTTTACAATGCATA+TGG + Chr4:68045311-68045330 MsG0480022086.01.T01:intron 30.0%
ATTCTTACACAACAGCAAAT+AGG - Chr4:68045293-68045312 None:intergenic 30.0%
CACATTTGATTAGAATGAGA+AGG - Chr4:68046311-68046330 None:intergenic 30.0%
CATAATATATATGCAAGGTC+TGG + Chr4:68045710-68045729 MsG0480022086.01.T01:intron 30.0%
CATTTGATTAGAATGAGAAG+GGG - Chr4:68046309-68046328 None:intergenic 30.0%
GGTTGAAGTATGTGATTAAA+TGG + Chr4:68045423-68045442 MsG0480022086.01.T01:intron 30.0%
TAATATATATGCAAGGTCTG+GGG + Chr4:68045712-68045731 MsG0480022086.01.T01:intron 30.0%
TCAAAAGTCAACAAACTAAG+TGG - Chr4:68045360-68045379 None:intergenic 30.0%
TTCAGTAATTAGTGATTCAG+TGG + Chr4:68045550-68045569 MsG0480022086.01.T01:intron 30.0%
! CTTTTCTTTCTCCAATTCAA+TGG - Chr4:68044889-68044908 None:intergenic 30.0%
!!! GCGCTATTTTTTTTTTTTGG+TGG - Chr4:68045753-68045772 None:intergenic 30.0%
ACTTCTGGTATAGAATGAGA+AGG - Chr4:68045795-68045814 None:intergenic 35.0%
AGGTACCATATGCATTGTAA+AGG - Chr4:68045319-68045338 None:intergenic 35.0%
AGTAGCGCTATTTAGTTAGT+GGG - Chr4:68045653-68045672 None:intergenic 35.0%
CAGTTACTCAGATATAGTGA+TGG + Chr4:68045894-68045913 MsG0480022086.01.T01:intron 35.0%
CTAATGATTCGCTTGCTTAT+TGG - Chr4:68045818-68045837 None:intergenic 35.0%
TGAATTGGAGAAAGAAAAGC+AGG + Chr4:68044890-68044909 MsG0480022086.01.T01:CDS 35.0%
TGCAAAATTGAAGCAGTGTA+TGG + Chr4:68044800-68044819 MsG0480022086.01.T01:CDS 35.0%
!! ACTCAGATATAGTGATGGAT+TGG + Chr4:68045899-68045918 MsG0480022086.01.T01:intron 35.0%
!! CTCAGATATAGTGATGGATT+GGG + Chr4:68045900-68045919 MsG0480022086.01.T01:intron 35.0%
!! CTGTGTGTTTGACTTCTTTT+GGG + Chr4:68045458-68045477 MsG0480022086.01.T01:intron 35.0%
!! GATGATGGGTTTGTAGTAAT+CGG - Chr4:68046258-68046277 None:intergenic 35.0%
!! GCCTAGCTATTTAGTTTGAT+CGG + Chr4:68045851-68045870 MsG0480022086.01.T01:intron 35.0%
!! TCTGTGTGTTTGACTTCTTT+TGG + Chr4:68045457-68045476 MsG0480022086.01.T01:intron 35.0%
!!! TTTTTTTTTTTGGTGGTGGC+CGG - Chr4:68045746-68045765 None:intergenic 35.0%
AACCGTCTCCTTTAATGAAC+AGG - Chr4:68046115-68046134 None:intergenic 40.0%
ACAATTCTCCCATTCAACCA+AGG - Chr4:68046147-68046166 None:intergenic 40.0%
ACTGTGTATCAGTGTATGAG+AGG + Chr4:68045110-68045129 MsG0480022086.01.T01:intron 40.0%
AGAGCAGGTTACCATTGAAT+TGG + Chr4:68044875-68044894 MsG0480022086.01.T01:CDS 40.0%
ATCAGTGTATGAGAGGTTGA+GGG + Chr4:68045117-68045136 MsG0480022086.01.T01:intron 40.0%
ATGGGAGAATTGTGTGAAAG+AGG + Chr4:68046154-68046173 MsG0480022086.01.T01:CDS 40.0%
CAAATAGGCTTAGCTAGCTA+AGG - Chr4:68045278-68045297 None:intergenic 40.0%
GAACTAGTTAGCACAGACAA+AGG - Chr4:68045533-68045552 None:intergenic 40.0%
GAGTAGCGCTATTTAGTTAG+TGG - Chr4:68045654-68045673 None:intergenic 40.0%
GATGAAGAAGAAGGAGAATG+CGG + Chr4:68044660-68044679 MsG0480022086.01.T01:CDS 40.0%
GCAGTGTATGGATGAACATT+CGG + Chr4:68044812-68044831 MsG0480022086.01.T01:CDS 40.0%
GCCGATCAAACTAAATAGCT+AGG - Chr4:68045855-68045874 None:intergenic 40.0%
GGAGAAAGAAAAGCAGGATT+TGG + Chr4:68044896-68044915 MsG0480022086.01.T01:CDS 40.0%
GGATTCTGCCTATTCATGAA+AGG + Chr4:68044681-68044700 MsG0480022086.01.T01:CDS 40.0%
TACCTAGTGCTTGTTTGGTT+TGG + Chr4:68045334-68045353 MsG0480022086.01.T01:intron 40.0%
TATCAGTGTATGAGAGGTTG+AGG + Chr4:68045116-68045135 MsG0480022086.01.T01:intron 40.0%
TATGGTACCTAGTGCTTGTT+TGG + Chr4:68045329-68045348 MsG0480022086.01.T01:intron 40.0%
TGCCTGTTCATTAAAGGAGA+CGG + Chr4:68046110-68046129 MsG0480022086.01.T01:CDS 40.0%
TTATGAACCCATTCTTAGGG+CGG + Chr4:68044839-68044858 MsG0480022086.01.T01:CDS 40.0%
TTCTGCCTATTCATGAAAGG+AGG + Chr4:68044684-68044703 MsG0480022086.01.T01:CDS 40.0%
! ACTCGTTTGTTGCAGTGTAT+GGG + Chr4:68046224-68046243 MsG0480022086.01.T01:CDS 40.0%
!! AGGGGATTCTTGTTGATTCT+CGG - Chr4:68044940-68044959 None:intergenic 40.0%
!! TCCTTTTCAGCTTGCTTTGA+GGG - Chr4:68046029-68046048 None:intergenic 40.0%
!! TTTAGTTTGATCGGCTCACT+TGG + Chr4:68045860-68045879 MsG0480022086.01.T01:intron 40.0%
!!! TTTTTTTTTTGGTGGTGGCC+GGG - Chr4:68045745-68045764 None:intergenic 40.0%
AACCGCCTCCTTTCATGAAT+AGG - Chr4:68044692-68044711 None:intergenic 45.0%
CAAAGACACGTTCATCGCTT+GGG + Chr4:68044713-68044732 MsG0480022086.01.T01:CDS 45.0%
CAGAGACACCTTGGTTGAAT+GGG + Chr4:68046136-68046155 MsG0480022086.01.T01:CDS 45.0%
CATACACTGCAACAAACGAG+TGG - Chr4:68046225-68046244 None:intergenic 45.0%
GAATTGTGTGAAAGAGGCGA+AGG + Chr4:68046160-68046179 MsG0480022086.01.T01:CDS 45.0%
GGGTTCTGCCTGTTCATTAA+AGG + Chr4:68046104-68046123 MsG0480022086.01.T01:CDS 45.0%
GGGTTTGTAGTAATCGGAAC+TGG - Chr4:68046252-68046271 None:intergenic 45.0%
TACGAAGGCCGAAAACAAAG+AGG + Chr4:68046067-68046086 MsG0480022086.01.T01:CDS 45.0%
TCGCTTGCTTATTGGACTTC+TGG - Chr4:68045810-68045829 None:intergenic 45.0%
TGATTGGGAAGAGGATCTGA+TGG - Chr4:68044591-68044610 None:intergenic 45.0%
TGCCTATTCATGAAAGGAGG+CGG + Chr4:68044687-68044706 MsG0480022086.01.T01:CDS 45.0%
TTCTTCATCTTCCTCGCCTT+CGG - Chr4:68044649-68044668 None:intergenic 45.0%
! AGGATCTGATGGTTTGGCAT+CGG - Chr4:68044580-68044599 None:intergenic 45.0%
! ATGGTTTGGCATCGGATTTG+GGG - Chr4:68044572-68044591 None:intergenic 45.0%
! CACTCGTTTGTTGCAGTGTA+TGG + Chr4:68046223-68046242 MsG0480022086.01.T01:CDS 45.0%
! GATGGTTTGGCATCGGATTT+GGG - Chr4:68044573-68044592 None:intergenic 45.0%
! TCTTGTTGAAAAGTGCGCTG+AGG + Chr4:68044773-68044792 MsG0480022086.01.T01:CDS 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG + Chr4:68045681-68045700 MsG0480022086.01.T01:intron 45.0%
! TGATGGTTTGGCATCGGATT+TGG - Chr4:68044574-68044593 None:intergenic 45.0%
!! CGGATTTGGGGTTTTCCAAT+GGG - Chr4:68044560-68044579 None:intergenic 45.0%
!! CTCCTTTTCAGCTTGCTTTG+AGG - Chr4:68046030-68046049 None:intergenic 45.0%
!! GGATTCTTGTTGATTCTCGG+CGG - Chr4:68044937-68044956 None:intergenic 45.0%
!! TCGGATTTGGGGTTTTCCAA+TGG - Chr4:68044561-68044580 None:intergenic 45.0%
!! TTACCGTTGACTCCAACTCA+CGG - Chr4:68045998-68046017 None:intergenic 45.0%
!! TTGGGGTTTTCCAATGGGTT+CGG - Chr4:68044555-68044574 None:intergenic 45.0%
!! ATAAAAACAATAATTTAAAA+AGG - Chr4:68044995-68045014 None:intergenic 5.0%
!! TAAAAACAATAATTTAAAAA+GGG - Chr4:68044994-68045013 None:intergenic 5.0%
AGAGGCGAAGGAGAAGAAAG+AGG + Chr4:68046172-68046191 MsG0480022086.01.T01:CDS 50.0%
ATGCTTCTCCGCCCTAAGAA+TGG - Chr4:68044850-68044869 None:intergenic 50.0%
CCGAAAACAAAGAGGAGGAG+GGG + Chr4:68046075-68046094 MsG0480022086.01.T01:CDS 50.0%
CGAAAACAAAGAGGAGGAGG+GGG + Chr4:68046076-68046095 MsG0480022086.01.T01:CDS 50.0%
GAAGGCCGAAAACAAAGAGG+AGG + Chr4:68046070-68046089 MsG0480022086.01.T01:CDS 50.0%
GCAAAGACACGTTCATCGCT+TGG + Chr4:68044712-68044731 MsG0480022086.01.T01:CDS 50.0%
GCAGAGACACCTTGGTTGAA+TGG + Chr4:68046135-68046154 MsG0480022086.01.T01:CDS 50.0%
GCCCTCAAAGCAAGCTGAAA+AGG + Chr4:68046025-68046044 MsG0480022086.01.T01:CDS 50.0%
GCCGAAAACAAAGAGGAGGA+GGG + Chr4:68046074-68046093 MsG0480022086.01.T01:CDS 50.0%
GGCCAAACCAAACAAGCACT+AGG - Chr4:68045339-68045358 None:intergenic 50.0%
GGCGAGGAAGATGAAGAAGA+AGG + Chr4:68044651-68044670 MsG0480022086.01.T01:CDS 50.0%
GGGAAGAGGATCTGATGGTT+TGG - Chr4:68044586-68044605 None:intergenic 50.0%
TCTTCCCAATCAACCACCAC+CGG + Chr4:68044599-68044618 MsG0480022086.01.T01:CDS 50.0%
TGAAAAGGAGCAACCAGCTC+CGG + Chr4:68046040-68046059 MsG0480022086.01.T01:CDS 50.0%
TGCTTCTCCGCCCTAAGAAT+GGG - Chr4:68044849-68044868 None:intergenic 50.0%
! AAGGTCTGGGGTTCAAATCC+CGG + Chr4:68045724-68045743 MsG0480022086.01.T01:intron 50.0%
! CGCTTCTCGGTCTTGATGAT+GGG - Chr4:68046272-68046291 None:intergenic 50.0%
! TGACCGTGAGTTGGAGTCAA+CGG + Chr4:68045992-68046011 MsG0480022086.01.T01:CDS 50.0%
!! ACCTCGCTGAAAAGTGCTCT+GGG + Chr4:68046195-68046214 MsG0480022086.01.T01:CDS 50.0%
!! ATGGATTGGGACGAGACACT+GGG + Chr4:68045913-68045932 MsG0480022086.01.T01:intron 50.0%
!! TTCTTGTTGATTCTCGGCGG+TGG - Chr4:68044934-68044953 None:intergenic 50.0%
!!! CCCCTCCTCCTCTTTGTTTT+CGG - Chr4:68046078-68046097 None:intergenic 50.0%
AAAGAGGAGGAGGGGGATTG+CGG + Chr4:68046083-68046102 MsG0480022086.01.T01:CDS 55.0%
ACTCCAACTCACGGTCACTG+CGG - Chr4:68045989-68046008 None:intergenic 55.0%
AGACGGTTGCAGAGACACCT+TGG + Chr4:68046127-68046146 MsG0480022086.01.T01:CDS 55.0%
CCAACTGAGCTAAGCTCACG+AGG - Chr4:68045679-68045698 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG + Chr4:68045676-68045695 MsG0480022086.01.T01:intron 55.0%
GCGCTTCTCGGTCTTGATGA+TGG - Chr4:68046273-68046292 None:intergenic 55.0%
GGCCGAAAACAAAGAGGAGG+AGG + Chr4:68046073-68046092 MsG0480022086.01.T01:CDS 55.0%
TTCATCTTCCTCGCCTTCGG+CGG - Chr4:68044646-68044665 None:intergenic 55.0%
TTGCTCTTCGACGCGCTTCT+CGG - Chr4:68046285-68046304 None:intergenic 55.0%
! AGACTCCGGTGGTGGTTGAT+TGG - Chr4:68044607-68044626 None:intergenic 55.0%
! GACTCCGGTGGTGGTTGATT+GGG - Chr4:68044606-68044625 None:intergenic 55.0%
! GGAGAAGCATGCTGAAGAGC+AGG + Chr4:68044860-68044879 MsG0480022086.01.T01:CDS 55.0%
! GGTGGTGGTTGATTGGGAAG+AGG - Chr4:68044600-68044619 None:intergenic 55.0%
! TTTTCGGCCTTCGTAGCCTC+CGG - Chr4:68046062-68046081 None:intergenic 55.0%
!! CCTCGCTGAAAAGTGCTCTG+GGG + Chr4:68046196-68046215 MsG0480022086.01.T01:CDS 55.0%
!! GACCTCGCTGAAAAGTGCTC+TGG + Chr4:68046194-68046213 MsG0480022086.01.T01:CDS 55.0%
!! GATGGATTGGGACGAGACAC+TGG + Chr4:68045912-68045931 MsG0480022086.01.T01:intron 55.0%
!! GGGTTTTCCAATGGGTTCGG+CGG - Chr4:68044552-68044571 None:intergenic 55.0%
!! GTTTCTGGTTCGGGAGACTC+CGG - Chr4:68044621-68044640 None:intergenic 55.0%
!! TTGTTGATTCTCGGCGGTGG+CGG - Chr4:68044931-68044950 None:intergenic 55.0%
AAAGGAGCAACCAGCTCCGG+AGG + Chr4:68046043-68046062 MsG0480022086.01.T01:CDS 60.0%
AAGAGGAGGAGGGGGATTGC+GGG + Chr4:68046084-68046103 MsG0480022086.01.T01:CDS 60.0%
GAACCGCAGTGACCGTGAGT+TGG + Chr4:68045983-68046002 MsG0480022086.01.T01:intron 60.0%
!! CCCCAGAGCACTTTTCAGCG+AGG - Chr4:68046199-68046218 None:intergenic 60.0%
!! TTCGGCGGCGGTTTCTGGTT+CGG - Chr4:68044631-68044650 None:intergenic 60.0%
ACCAGCTCCGGAGGCTACGA+AGG + Chr4:68046052-68046071 MsG0480022086.01.T01:CDS 65.0%
ATCTTCCTCGCCTTCGGCGG+CGG - Chr4:68044643-68044662 None:intergenic 65.0%
GAACCAGAAACCGCCGCCGA+AGG + Chr4:68044630-68044649 MsG0480022086.01.T01:CDS 65.0%
! TTGATTCTCGGCGGTGGCGG+CGG - Chr4:68044928-68044947 None:intergenic 65.0%
!! TCGGCGGCGGTTTCTGGTTC+GGG - Chr4:68044630-68044649 None:intergenic 65.0%
!! TCTGGTTCGGGAGACTCCGG+TGG - Chr4:68044618-68044637 None:intergenic 65.0%
AGAAACCGCCGCCGAAGGCG+AGG + Chr4:68044635-68044654 MsG0480022086.01.T01:CDS 70.0%
GCCTTCGTAGCCTCCGGAGC+TGG - Chr4:68046056-68046075 None:intergenic 70.0%
GGTTCGGGAGACTCCGGTGG+TGG - Chr4:68044615-68044634 None:intergenic 70.0%
GTCGACGCCGCCGAACCCAT+TGG + Chr4:68044542-68044561 MsG0480022086.01.T01:CDS 70.0%
TCGCCTTCGGCGGCGGTTTC+TGG - Chr4:68044636-68044655 None:intergenic 70.0%
! ATTCTCGGCGGTGGCGGCGG+CGG - Chr4:68044925-68044944 None:intergenic 75.0%
!! CTCGGCGGTGGCGGCGGCGG+CGG - Chr4:68044922-68044941 None:intergenic 90.0%
Chromosome Type Strat End Strand Name
Chr4 gene 68044540 68046331 68044540 ID=MsG0480022086.01;Name=MsG0480022086.01
Chr4 mRNA 68044540 68046331 68044540 ID=MsG0480022086.01.T01;Parent=MsG0480022086.01;Name=MsG0480022086.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|256
Chr4 exon 68044540 68044970 68044540 ID=MsG0480022086.01.T01:exon:18409;Parent=MsG0480022086.01.T01
Chr4 exon 68045992 68046331 68045992 ID=MsG0480022086.01.T01:exon:18410;Parent=MsG0480022086.01.T01
Chr4 CDS 68044540 68044970 68044540 ID=MsG0480022086.01.T01:cds;Parent=MsG0480022086.01.T01
Chr4 CDS 68045992 68046331 68045992 ID=MsG0480022086.01.T01:cds;Parent=MsG0480022086.01.T01
Gene Sequence

>MsG0480022086.01.T01

ATGTCGACGCCGCCGAACCCATTGGAAAACCCCAAATCCGATGCCAAACCATCAGATCCTCTTCCCAATCAACCACCACCGGAGTCTCCCGAACCAGAAACCGCCGCCGAAGGCGAGGAAGATGAAGAAGAAGGAGAATGCGGATTCTGCCTATTCATGAAAGGAGGCGGTTGCAAAGACACGTTCATCGCTTGGGAAGATTGCGTGAAAGAAGCTGAAGACAAAAATGAAGATCTTGTTGAAAAGTGCGCTGAGGTTACTGCAAAATTGAAGCAGTGTATGGATGAACATTCGGATTATTATGAACCCATTCTTAGGGCGGAGAAGCATGCTGAAGAGCAGGTTACCATTGAATTGGAGAAAGAAAAGCAGGATTTGGCCGCCGCCGCCGCCACCGCCGAGAATCAACAAGAATCCCCTTCTAATCAAAATGACCGTGAGTTGGAGTCAACGGTAATTTCAACGCCCTCAAAGCAAGCTGAAAAGGAGCAACCAGCTCCGGAGGCTACGAAGGCCGAAAACAAAGAGGAGGAGGGGGATTGCGGGTTCTGCCTGTTCATTAAAGGAGACGGTTGCAGAGACACCTTGGTTGAATGGGAGAATTGTGTGAAAGAGGCGAAGGAGAAGAAAGAGGACCTCGCTGAAAAGTGCTCTGGGGTCACCACTCGTTTGTTGCAGTGTATGGGTTCCAGTTCCGATTACTACAAACCCATCATCAAGACCGAGAAGCGCGTCGAAGAGCAAGCCCCTTCTCATTCTAATCAAATGTGA
Protein sequence

>MsG0480022086.01.T01

MSTPPNPLENPKSDAKPSDPLPNQPPPESPEPETAAEGEEDEEEGECGFCLFMKGGGCKDTFIAWEDCVKEAEDKNEDLVEKCAEVTAKLKQCMDEHSDYYEPILRAEKHAEEQVTIELEKEKQDLAAAAATAENQQESPSNQNDRELESTVISTPSKQAEKEQPAPEATKAENKEEEGDCGFCLFIKGDGCRDTLVEWENCVKEAKEKKEDLAEKCSGVTTRLLQCMGSSSDYYKPIIKTEKRVEEQAPSHSNQM*