AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029047.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029047.01.T01 MTR_5g080880 99.213 254 2 0 1 254 1 254 0.0 522
MsG0580029047.01.T01 MTR_5g080870 66.275 255 51 6 1 255 1 220 1.39e-109 319
MsG0580029047.01.T01 MTR_7g063520 48.800 250 109 5 8 254 20 253 6.61e-73 223
MsG0580029047.01.T01 MTR_4g006560 41.509 159 86 3 97 254 83 235 1.61e-31 116
MsG0580029047.01.T01 MTR_1g013120 32.500 240 142 9 24 254 4 232 6.34e-26 102
MsG0580029047.01.T01 MTR_5g093000 32.749 171 89 7 92 253 59 212 3.54e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029047.01.T01 AT3G55605 50.370 270 105 8 1 254 1 257 4.54e-71 219
MsG0580029047.01.T01 AT5G05990 45.833 264 121 5 5 254 3 258 2.37e-69 214
MsG0580029047.01.T01 AT2G39795 52.792 197 87 3 60 254 57 249 6.40e-64 200
MsG0580029047.01.T01 AT5G02050 43.750 272 129 5 1 254 1 266 6.91e-59 188
MsG0580029047.01.T01 AT2G39790 35.249 261 138 6 1 253 1 238 7.12e-39 135
MsG0580029047.01.T01 AT1G15870 33.824 204 121 5 54 254 48 240 3.47e-27 105
MsG0580029047.01.T01 AT1G80720 31.863 204 125 6 54 254 48 240 2.63e-22 92.4
MsG0580029047.01.T01 AT1G80720 32.105 190 115 6 68 254 10 188 4.26e-22 90.9
MsG0580029047.01.T01 AT4G31930 32.632 190 114 7 68 253 53 232 1.06e-20 88.2
MsG0580029047.01.T01 AT4G32605 34.146 164 89 6 97 253 66 217 7.97e-18 80.1

Find 56 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATTATCAACTATCTTAAA+TGG 0.175868 5:-90874388 None:intergenic
TCCACAAGTACTTAGAAATA+AGG 0.241404 5:+90875156 MsG0580029047.01.T01:CDS
AGTGTTATTGTCTGATTTCC+TGG 0.252098 5:-90874409 None:intergenic
ATCAGACAATAACACTTAAA+AGG 0.289378 5:+90874416 MsG0580029047.01.T01:CDS
TAAGAAGGATGGGCCCTCTT+TGG 0.320568 5:+90874582 MsG0580029047.01.T01:CDS
CCACAAGTACTTAGAAATAA+GGG 0.328236 5:+90875157 MsG0580029047.01.T01:CDS
TGAGGTTCACATGCCTGATT+TGG 0.337495 5:+90874465 MsG0580029047.01.T01:CDS
CTGTCGATAACAATCTCATC+TGG 0.355760 5:-90874625 None:intergenic
CCAAGTCAAGCACTTTCGTT+AGG 0.373305 5:+90871770 MsG0580029047.01.T01:CDS
AGAAGTACATACTGAAAATC+TGG 0.385204 5:-90874700 None:intergenic
GCTCAGGAAACCGATGATTT+CGG 0.391310 5:+90871979 MsG0580029047.01.T01:CDS
CACATGCCTGATTTGGCTAC+TGG 0.399277 5:+90874472 MsG0580029047.01.T01:CDS
ATCTTAAATGGGAAATTGCT+TGG 0.407574 5:-90874376 None:intergenic
CCCTTATTTCTAAGTACTTG+TGG 0.408675 5:-90875157 None:intergenic
CGCCGCCGTCAATCGATACC+AGG 0.411836 5:+90871855 MsG0580029047.01.T01:CDS
TTCAGTCAGTGTTTCTAAGA+AGG 0.415683 5:+90874567 MsG0580029047.01.T01:CDS
TGCAAGAAATTTGTTGTGCT+TGG 0.436674 5:-90875188 None:intergenic
TGAAAATCTGGCCCGTCATA+AGG 0.439441 5:-90874688 None:intergenic
CACGGCGCGATGACCCGGTT+GGG 0.442829 5:-90871827 None:intergenic
TCTTCTCCGTTTCATCGAAT+CGG 0.447658 5:+90871945 MsG0580029047.01.T01:CDS
CAAGGATAGTAGAGAATACT+TGG 0.453079 5:+90875226 MsG0580029047.01.T01:CDS
GATTATCAACTATCTTAAAT+GGG 0.456111 5:-90874387 None:intergenic
TCTTCACCAGTAGCCAAATC+AGG 0.462113 5:-90874478 None:intergenic
TTAGAAACACTGACTGAAAG+TGG 0.463235 5:-90874562 None:intergenic
GTCAGTGTTTCTAAGAAGGA+TGG 0.492712 5:+90874571 MsG0580029047.01.T01:CDS
GAGAAGAATATCGTCGGAGG+TGG 0.494464 5:-90871929 None:intergenic
ATCGGAAATCACTTGTGCTC+AGG 0.496436 5:+90871963 MsG0580029047.01.T01:CDS
TGATCATCAAGATGATGATA+AGG 0.496458 5:+90874504 MsG0580029047.01.T01:CDS
TTTAAGATAGTTGATAATCC+AGG 0.504200 5:+90874391 MsG0580029047.01.T01:CDS
CAAGTCAAGCACTTTCGTTA+GGG 0.512325 5:+90871771 MsG0580029047.01.T01:CDS
GAAAGCACGGCGCGATGACC+CGG 0.517251 5:-90871832 None:intergenic
TGATTTCCGATTCGATGAAA+CGG 0.520694 5:-90871951 None:intergenic
ACTTACAGCGCCGAAATCAT+CGG 0.522056 5:-90871989 None:intergenic
ACACTTAAAAGGACTTATCA+AGG 0.527097 5:+90874427 MsG0580029047.01.T01:CDS
ATGAACAGATTCCTTATGAC+GGG 0.541918 5:+90874677 MsG0580029047.01.T01:CDS
ATTGACGGCGGCGGAAAGCA+CGG 0.549833 5:-90871845 None:intergenic
TGAACCCTGGTATCGATTGA+CGG 0.565956 5:-90871860 None:intergenic
AGGTGAAGAAATTCAAGTTG+AGG 0.571505 5:+90874447 MsG0580029047.01.T01:CDS
AGAAGAATATCGTCGGAGGT+GGG 0.577026 5:-90871928 None:intergenic
GCACGGCGCGATGACCCGGT+TGG 0.590649 5:-90871828 None:intergenic
TGTCGATAACAATCTCATCT+GGG 0.591480 5:-90874624 None:intergenic
CCTAACGAAAGTGCTTGACT+TGG 0.591991 5:-90871770 None:intergenic
CTGGTATCGATTGACGGCGG+CGG 0.595525 5:-90871854 None:intergenic
TCAGTGTTTCTAAGAAGGAT+GGG 0.595788 5:+90874572 MsG0580029047.01.T01:CDS
CACAAGTACTTAGAAATAAG+GGG 0.598764 5:+90875158 MsG0580029047.01.T01:CDS
ACACAACTGAACTCCAAAGA+GGG 0.618264 5:-90874595 None:intergenic
GATGAACAGATTCCTTATGA+CGG 0.641036 5:+90874676 MsG0580029047.01.T01:CDS
ATAGTAGAGAATACTTGGTG+TGG 0.641539 5:+90875231 MsG0580029047.01.T01:CDS
AACACAACTGAACTCCAAAG+AGG 0.648826 5:-90874596 None:intergenic
GCCGCCGTCAATCGATACCA+GGG 0.649914 5:+90871856 MsG0580029047.01.T01:CDS
ACAGAAGAAAAGTGAAACGT+AGG 0.650656 5:-90871889 None:intergenic
GAAACGGAGAAGAATATCGT+CGG 0.655705 5:-90871935 None:intergenic
ACGTAGGAACAAATGAACCC+TGG 0.662093 5:-90871873 None:intergenic
ACGGAGAAGAATATCGTCGG+AGG 0.701354 5:-90871932 None:intergenic
GCATGAGTACATGATCAACA+AGG 0.729521 5:+90875208 MsG0580029047.01.T01:CDS
ACCCTGGTATCGATTGACGG+CGG 0.780259 5:-90871857 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATTTAATTTTTCTGTT+TGG - Chr5:90872765-90872784 None:intergenic 10.0%
!! AAATGTTGAAAATTTGAATT+TGG + Chr5:90874277-90874296 MsG0580029047.01.T01:intron 15.0%
!! AAGAGTAAAAAAAATTATCT+TGG + Chr5:90873538-90873557 MsG0580029047.01.T01:intron 15.0%
!! TAAATATGATTATCTTAGTT+TGG + Chr5:90873158-90873177 MsG0580029047.01.T01:intron 15.0%
!! TGAAATGAAACTTATTATTA+AGG - Chr5:90873911-90873930 None:intergenic 15.0%
!! TTATTTATGAAGAAAGAATT+TGG + Chr5:90873016-90873035 MsG0580029047.01.T01:intron 15.0%
!!! AAATGTTGAAAAAATGATTT+TGG + Chr5:90874332-90874351 MsG0580029047.01.T01:intron 15.0%
!!! CTTTTACTCTATTAATTTAA+TGG + Chr5:90873589-90873608 MsG0580029047.01.T01:intron 15.0%
!! ATCATGAGTCTAATAAATTA+AGG - Chr5:90872023-90872042 None:intergenic 20.0%
!! ATTATAATAAAAGACCTGTT+TGG + Chr5:90872391-90872410 MsG0580029047.01.T01:intron 20.0%
!! GAAACTTATTATTAAGGTAT+AGG - Chr5:90873905-90873924 None:intergenic 20.0%
!! GATTATCAACTATCTTAAAT+GGG - Chr5:90874390-90874409 None:intergenic 20.0%
!! GTTAAGGTTAAAAAAGTTTA+TGG - Chr5:90872943-90872962 None:intergenic 20.0%
!! TAAATATGAATGACATACAA+AGG - Chr5:90872798-90872817 None:intergenic 20.0%
!!! AAAATGATTTTGGTTGATAT+AGG + Chr5:90874342-90874361 MsG0580029047.01.T01:intron 20.0%
!!! AAATGATTTTGGTTGATATA+GGG + Chr5:90874343-90874362 MsG0580029047.01.T01:intron 20.0%
!!! AACAATCTGAAATTTAGATT+GGG + Chr5:90874933-90874952 MsG0580029047.01.T01:intron 20.0%
!!! AAGAATTGAAAGTATACAAA+CGG - Chr5:90875058-90875077 None:intergenic 20.0%
!!! ACAAGATTTTTAAAATAGAC+AGG - Chr5:90873394-90873413 None:intergenic 20.0%
!!! CAAGATTTTTAAAATAGACA+GGG - Chr5:90873393-90873412 None:intergenic 20.0%
!!! TTAAAACTTTTAGCTTATCA+TGG + Chr5:90872280-90872299 MsG0580029047.01.T01:intron 20.0%
! AAAATTAACAGTTACCAAAC+AGG - Chr5:90872408-90872427 None:intergenic 25.0%
! AAGAAGATAGGATATTTAAC+AGG + Chr5:90874054-90874073 MsG0580029047.01.T01:intron 25.0%
! AATCCTTAAAATTAGTCAAG+TGG + Chr5:90873563-90873582 MsG0580029047.01.T01:intron 25.0%
! ACAAAAGACTACAAAACAAA+AGG - Chr5:90874152-90874171 None:intergenic 25.0%
! AGAAAATTTAGTGAGAAACT+GGG + Chr5:90873421-90873440 MsG0580029047.01.T01:intron 25.0%
! ATCAGACAATAACACTTAAA+AGG + Chr5:90874416-90874435 MsG0580029047.01.T01:CDS 25.0%
! ATCCTTAAAATTAGTCAAGT+GGG + Chr5:90873564-90873583 MsG0580029047.01.T01:intron 25.0%
! ATTTATATATAGGGTCTGTT+TGG + Chr5:90872080-90872099 MsG0580029047.01.T01:intron 25.0%
! CAACAAGATAATCATTGTTA+AGG - Chr5:90872959-90872978 None:intergenic 25.0%
! CATTGATATCAAGTTTCTAT+TGG + Chr5:90873929-90873948 MsG0580029047.01.T01:intron 25.0%
! CTAATTCAAGTAGTTTATAG+CGG + Chr5:90872477-90872496 MsG0580029047.01.T01:intron 25.0%
! GGATTATCAACTATCTTAAA+TGG - Chr5:90874391-90874410 None:intergenic 25.0%
! GGTAAAAATAAGTTATAAGC+TGG - Chr5:90872216-90872235 None:intergenic 25.0%
! TAGAAAATTTAGTGAGAAAC+TGG + Chr5:90873420-90873439 MsG0580029047.01.T01:intron 25.0%
! TATCAAAAGATGCTATAACA+TGG - Chr5:90872237-90872256 None:intergenic 25.0%
! TATTAAGGTATAGGAACAAA+AGG - Chr5:90873896-90873915 None:intergenic 25.0%
! TTAAATATCCTATCTTCTTC+CGG - Chr5:90874053-90874072 None:intergenic 25.0%
! TTTATGAAGAAAGAATTTGG+CGG + Chr5:90873019-90873038 MsG0580029047.01.T01:intron 25.0%
! TTTCTAATGTTTGTGTAGTT+TGG + Chr5:90873458-90873477 MsG0580029047.01.T01:intron 25.0%
!! ACATCAGTGTTGTTAAATAA+AGG + Chr5:90874780-90874799 MsG0580029047.01.T01:intron 25.0%
!! ATATGATTATCTTAGTTTGG+AGG + Chr5:90873161-90873180 MsG0580029047.01.T01:intron 25.0%
!! CAATTTTACCCTGTAATATT+AGG + Chr5:90872308-90872327 MsG0580029047.01.T01:intron 25.0%
!! GAACAATCTGAAATTTAGAT+TGG + Chr5:90874932-90874951 MsG0580029047.01.T01:intron 25.0%
!! GTGATTGAATTTGTAGTTTT+TGG - Chr5:90872846-90872865 None:intergenic 25.0%
!! TTCAATTCTTGTTGTATCTA+AGG + Chr5:90875068-90875087 MsG0580029047.01.T01:intron 25.0%
!!! AAAAAGTTTATGGCTTTGTT+TGG - Chr5:90872933-90872952 None:intergenic 25.0%
!!! AATTGTCTAATTTTTTCCAG+TGG + Chr5:90873303-90873322 MsG0580029047.01.T01:intron 25.0%
!!! TAAGACATAAATTTGGTTTG+TGG + Chr5:90872341-90872360 MsG0580029047.01.T01:intron 25.0%
!!! TTATTTCTATGAGCATATAG+CGG + Chr5:90872443-90872462 MsG0580029047.01.T01:intron 25.0%
!!! TTTAAGATAGTTGATAATCC+AGG + Chr5:90874391-90874410 MsG0580029047.01.T01:CDS 25.0%
!!! TTTTTCAATTGAGATTGCAA+GGG - Chr5:90872560-90872579 None:intergenic 25.0%
!!! TTTTTGGTAAACTTAGCAAA+TGG - Chr5:90872830-90872849 None:intergenic 25.0%
!!! TTTTTTCAATTGAGATTGCA+AGG - Chr5:90872561-90872580 None:intergenic 25.0%
AAAGATTATCTTTCGTGAGA+GGG + Chr5:90873208-90873227 MsG0580029047.01.T01:intron 30.0%
ACACTTAAAAGGACTTATCA+AGG + Chr5:90874427-90874446 MsG0580029047.01.T01:CDS 30.0%
ATAGCTTATAGCTTATAAGC+TGG + Chr5:90872367-90872386 MsG0580029047.01.T01:intron 30.0%
ATCTGTTCATCAGAAAAATC+AGG - Chr5:90874667-90874686 None:intergenic 30.0%
ATCTTAAATGGGAAATTGCT+TGG - Chr5:90874379-90874398 None:intergenic 30.0%
ATTTAACAGGTTGTACAATC+TGG + Chr5:90874067-90874086 MsG0580029047.01.T01:intron 30.0%
CACAAGTACTTAGAAATAAG+GGG + Chr5:90875158-90875177 MsG0580029047.01.T01:CDS 30.0%
CACGAAAGATAATCTTTAGT+TGG - Chr5:90873205-90873224 None:intergenic 30.0%
CCACAAGTACTTAGAAATAA+GGG + Chr5:90875157-90875176 MsG0580029047.01.T01:CDS 30.0%
GTATTAATATGCTCAGAATC+AGG - Chr5:90874222-90874241 None:intergenic 30.0%
TAAAGATTATCTTTCGTGAG+AGG + Chr5:90873207-90873226 MsG0580029047.01.T01:intron 30.0%
TCAACTTATAGCTTATAAGC+TGG + Chr5:90872116-90872135 MsG0580029047.01.T01:intron 30.0%
TCCACAAGTACTTAGAAATA+AGG + Chr5:90875156-90875175 MsG0580029047.01.T01:CDS 30.0%
TGATATCAAGTTTCTATTGG+TGG + Chr5:90873932-90873951 MsG0580029047.01.T01:intron 30.0%
TGATCATCAAGATGATGATA+AGG + Chr5:90874504-90874523 MsG0580029047.01.T01:CDS 30.0%
TTATGAAGAAAGAATTTGGC+GGG + Chr5:90873020-90873039 MsG0580029047.01.T01:intron 30.0%
TTCGAAATATCCAAATTGGA+AGG - Chr5:90873368-90873387 None:intergenic 30.0%
TTCTCAAAAAGGATTCGAAT+GGG + Chr5:90873504-90873523 MsG0580029047.01.T01:intron 30.0%
TTTCTCAAAAAGGATTCGAA+TGG + Chr5:90873503-90873522 MsG0580029047.01.T01:intron 30.0%
TTTGTGTAGTTTGGAAACTT+AGG + Chr5:90873467-90873486 MsG0580029047.01.T01:intron 30.0%
! AACCCACTTGACTAATTTTA+AGG - Chr5:90873569-90873588 None:intergenic 30.0%
! AGAAGTACATACTGAAAATC+TGG - Chr5:90874703-90874722 None:intergenic 30.0%
! AGATTCTTCCATGTTTTACA+GGG + Chr5:90875106-90875125 MsG0580029047.01.T01:intron 30.0%
! ATCACTTTCTGTTTGAAGTT+GGG + Chr5:90873644-90873663 MsG0580029047.01.T01:intron 30.0%
! GCTAGCTTATTTGTAATGTT+TGG - Chr5:90872654-90872673 None:intergenic 30.0%
! GTAATGTTTGGTGAAATTAG+CGG - Chr5:90872642-90872661 None:intergenic 30.0%
! TAACTGATAAACTAGCTTAG+AGG - Chr5:90872895-90872914 None:intergenic 30.0%
! TTTTCAATTGAGATTGCAAG+GGG - Chr5:90872559-90872578 None:intergenic 30.0%
!! AATTTTCAGGGTTTTGTGTT+AGG + Chr5:90873240-90873259 MsG0580029047.01.T01:intron 30.0%
!! GCATTCAATGTTTTAAACCA+GGG + Chr5:90874755-90874774 MsG0580029047.01.T01:intron 30.0%
!! TAACATGATTGTTCTGAAAC+AGG - Chr5:90872162-90872181 None:intergenic 30.0%
!! TGGTTGGTAAGACATAAATT+TGG + Chr5:90872334-90872353 MsG0580029047.01.T01:intron 30.0%
!!! GGTGTGATTTTTTCTCAAAA+AGG + Chr5:90873493-90873512 MsG0580029047.01.T01:intron 30.0%
!!! TCTAATTTTTTCCAGTGGTT+TGG + Chr5:90873308-90873327 MsG0580029047.01.T01:intron 30.0%
!!! TTTACCTCAAGAGTTTTTTC+CGG + Chr5:90874019-90874038 MsG0580029047.01.T01:intron 30.0%
AACCAGAGCCTAATATTACA+GGG - Chr5:90872319-90872338 None:intergenic 35.0%
AAGATTATCTTTCGTGAGAG+GGG + Chr5:90873209-90873228 MsG0580029047.01.T01:intron 35.0%
AATCTACACCTCATTACCAT+CGG - Chr5:90873099-90873118 None:intergenic 35.0%
ACAGAAGAAAAGTGAAACGT+AGG - Chr5:90871892-90871911 None:intergenic 35.0%
AGAAACTGGGTGTGATTATT+TGG + Chr5:90873434-90873453 MsG0580029047.01.T01:intron 35.0%
ATCCAAATCCCTGTAAAACA+TGG - Chr5:90875117-90875136 None:intergenic 35.0%
ATGAACAGATTCCTTATGAC+GGG + Chr5:90874677-90874696 MsG0580029047.01.T01:CDS 35.0%
ATTGATCAGAAAAACCCAAC+CGG + Chr5:90871813-90871832 MsG0580029047.01.T01:CDS 35.0%
CAAGGATAGTAGAGAATACT+TGG + Chr5:90875226-90875245 MsG0580029047.01.T01:CDS 35.0%
CTTTCAAATAGCAGCTATAG+TGG + Chr5:90874879-90874898 MsG0580029047.01.T01:intron 35.0%
GATGAACAGATTCCTTATGA+CGG + Chr5:90874676-90874695 MsG0580029047.01.T01:CDS 35.0%
GGAGTTCGAAATATCCAAAT+TGG - Chr5:90873372-90873391 None:intergenic 35.0%
GTAGTTTGGAAACTTAGGAA+CGG + Chr5:90873472-90873491 MsG0580029047.01.T01:intron 35.0%
TGTCGATAACAATCTCATCT+GGG - Chr5:90874627-90874646 None:intergenic 35.0%
! AGTGTTATTGTCTGATTTCC+TGG - Chr5:90874412-90874431 None:intergenic 35.0%
! AGTTCTATGTACTTGTTGAG+GGG + Chr5:90873675-90873694 MsG0580029047.01.T01:intron 35.0%
! AGTTGAGTTCATCTAGTTTC+AGG + Chr5:90873839-90873858 MsG0580029047.01.T01:intron 35.0%
! ATAGTAGAGAATACTTGGTG+TGG + Chr5:90875231-90875250 MsG0580029047.01.T01:CDS 35.0%
! ATGGTAATGAGGTGTAGATT+GGG + Chr5:90873099-90873118 MsG0580029047.01.T01:intron 35.0%
! CAGTTCTATGTACTTGTTGA+GGG + Chr5:90873674-90873693 MsG0580029047.01.T01:intron 35.0%
! CTTGTTGTATCTAAGGCATA+TGG + Chr5:90875075-90875094 MsG0580029047.01.T01:intron 35.0%
! GAGATTCTTCCATGTTTTAC+AGG + Chr5:90875105-90875124 MsG0580029047.01.T01:intron 35.0%
! GATCACTTTCTGTTTGAAGT+TGG + Chr5:90873643-90873662 MsG0580029047.01.T01:intron 35.0%
! GTTGAGTTCATCTAGTTTCA+GGG + Chr5:90873840-90873859 MsG0580029047.01.T01:intron 35.0%
! TCACTTTCTGTTTGAAGTTG+GGG + Chr5:90873645-90873664 MsG0580029047.01.T01:intron 35.0%
! TCAGCTATAAGCTAGCTTAT+AGG + Chr5:90872684-90872703 MsG0580029047.01.T01:intron 35.0%
! TCAGTGTTTCTAAGAAGGAT+GGG + Chr5:90874572-90874591 MsG0580029047.01.T01:CDS 35.0%
! TCAGTTCTATGTACTTGTTG+AGG + Chr5:90873673-90873692 MsG0580029047.01.T01:intron 35.0%
! TGATTTCCGATTCGATGAAA+CGG - Chr5:90871954-90871973 None:intergenic 35.0%
! TTAGAAACACTGACTGAAAG+TGG - Chr5:90874565-90874584 None:intergenic 35.0%
! TTCAGTCAGTGTTTCTAAGA+AGG + Chr5:90874567-90874586 MsG0580029047.01.T01:CDS 35.0%
! TTCCATGTTTTACAGGGATT+TGG + Chr5:90875112-90875131 MsG0580029047.01.T01:intron 35.0%
!! AGGTGAAGAAATTCAAGTTG+AGG + Chr5:90874447-90874466 MsG0580029047.01.T01:CDS 35.0%
!! AGTGAGACTTTGAAATGTCT+TGG + Chr5:90873331-90873350 MsG0580029047.01.T01:intron 35.0%
!! CCCTTATTTCTAAGTACTTG+TGG - Chr5:90875160-90875179 None:intergenic 35.0%
!! CGCATTCAATGTTTTAAACC+AGG + Chr5:90874754-90874773 MsG0580029047.01.T01:intron 35.0%
!! CTATAGCACTTTTTGTGTAG+CGG + Chr5:90874904-90874923 MsG0580029047.01.T01:intron 35.0%
!! GTGAGACTTTGAAATGTCTT+GGG + Chr5:90873332-90873351 MsG0580029047.01.T01:intron 35.0%
!! TGAGACTTTGAAATGTCTTG+GGG + Chr5:90873333-90873352 MsG0580029047.01.T01:intron 35.0%
!! TGCAAGAAATTTGTTGTGCT+TGG - Chr5:90875191-90875210 None:intergenic 35.0%
!!! AGGGGTTGGTATTAATTTTC+AGG + Chr5:90873227-90873246 MsG0580029047.01.T01:intron 35.0%
!!! ATTTTGGTTGATATAGGGTG+AGG + Chr5:90874348-90874367 MsG0580029047.01.T01:intron 35.0%
!!! GGGGTTGGTATTAATTTTCA+GGG + Chr5:90873228-90873247 MsG0580029047.01.T01:intron 35.0%
!!! TTACCTCAAGAGTTTTTTCC+GGG + Chr5:90874020-90874039 MsG0580029047.01.T01:intron 35.0%
AACACAACTGAACTCCAAAG+AGG - Chr5:90874599-90874618 None:intergenic 40.0%
AAGTGATCTCTCAACAGAAC+AGG - Chr5:90873631-90873650 None:intergenic 40.0%
AATGAGGTGTAGATTGGGTA+GGG + Chr5:90873104-90873123 MsG0580029047.01.T01:intron 40.0%
ACACAACTGAACTCCAAAGA+GGG - Chr5:90874598-90874617 None:intergenic 40.0%
ACAGGTTGTACAATCTGGTT+TGG + Chr5:90874072-90874091 MsG0580029047.01.T01:intron 40.0%
CAACCAGAGCCTAATATTAC+AGG - Chr5:90872320-90872339 None:intergenic 40.0%
CAAGTCAAGCACTTTCGTTA+GGG + Chr5:90871771-90871790 MsG0580029047.01.T01:CDS 40.0%
CTGTCGATAACAATCTCATC+TGG - Chr5:90874628-90874647 None:intergenic 40.0%
GAAACGGAGAAGAATATCGT+CGG - Chr5:90871938-90871957 None:intergenic 40.0%
GACTCTTTGACAGAGTTACT+CGG - Chr5:90873059-90873078 None:intergenic 40.0%
GCATGAGTACATGATCAACA+AGG + Chr5:90875208-90875227 MsG0580029047.01.T01:CDS 40.0%
GCTTTCTCTTTCATCTTGTC+TGG + Chr5:90873867-90873886 MsG0580029047.01.T01:intron 40.0%
GTAGAATTCCGGAAGAAGAT+AGG + Chr5:90874042-90874061 MsG0580029047.01.T01:intron 40.0%
TAACAACACTGATGTAACCC+TGG - Chr5:90874775-90874794 None:intergenic 40.0%
TAATGAGGTGTAGATTGGGT+AGG + Chr5:90873103-90873122 MsG0580029047.01.T01:intron 40.0%
TACCCTGTAATATTAGGCTC+TGG + Chr5:90872314-90872333 MsG0580029047.01.T01:intron 40.0%
TCTTCTCCGTTTCATCGAAT+CGG + Chr5:90871945-90871964 MsG0580029047.01.T01:CDS 40.0%
TGATGTTGCAGTTCAGATTG+CGG + Chr5:90875012-90875031 MsG0580029047.01.T01:intron 40.0%
TTGATCAGAAAAACCCAACC+GGG + Chr5:90871814-90871833 MsG0580029047.01.T01:CDS 40.0%
! CAGGTTGTACAATCTGGTTT+GGG + Chr5:90874073-90874092 MsG0580029047.01.T01:intron 40.0%
! CTGTAATATTAGGCTCTGGT+TGG + Chr5:90872318-90872337 MsG0580029047.01.T01:intron 40.0%
! GATGGTAATGAGGTGTAGAT+TGG + Chr5:90873098-90873117 MsG0580029047.01.T01:intron 40.0%
! GCAAACTTTTGCTGCAAGTT+TGG + Chr5:90873963-90873982 MsG0580029047.01.T01:intron 40.0%
! GTCAGTGTTTCTAAGAAGGA+TGG + Chr5:90874571-90874590 MsG0580029047.01.T01:CDS 40.0%
! GTTCATCTAGTTTCAGGGTT+AGG + Chr5:90873845-90873864 MsG0580029047.01.T01:intron 40.0%
! GTTCTATGTACTTGTTGAGG+GGG + Chr5:90873676-90873695 MsG0580029047.01.T01:intron 40.0%
! GTTTTACAGGGATTTGGATG+AGG + Chr5:90875118-90875137 MsG0580029047.01.T01:intron 40.0%
! TTCTATGTACTTGTTGAGGG+GGG + Chr5:90873677-90873696 MsG0580029047.01.T01:intron 40.0%
!! CTCTGTCAAAGAGTCGTTTT+TGG + Chr5:90873064-90873083 MsG0580029047.01.T01:intron 40.0%
!! TTTCAGGGTTTTGTGTTAGG+AGG + Chr5:90873243-90873262 MsG0580029047.01.T01:intron 40.0%
!!! AGTTTTTCAAGGGGTGTTTG+TGG + Chr5:90873725-90873744 MsG0580029047.01.T01:intron 40.0%
!!! GTTTTTTCCGGGTAGAATTC+CGG + Chr5:90874031-90874050 MsG0580029047.01.T01:intron 40.0%
!!! TGCACTCTGGAGTTTTTCAA+GGG + Chr5:90873715-90873734 MsG0580029047.01.T01:intron 40.0%
!!! TTGCACTCTGGAGTTTTTCA+AGG + Chr5:90873714-90873733 MsG0580029047.01.T01:intron 40.0%
AAAGAATTTGGCGGGAGATG+GGG + Chr5:90873028-90873047 MsG0580029047.01.T01:intron 45.0%
AAAGTCTCACTCCAAACCAC+TGG - Chr5:90873322-90873341 None:intergenic 45.0%
ACGTAGGAACAAATGAACCC+TGG - Chr5:90871876-90871895 None:intergenic 45.0%
AGAAAGAATTTGGCGGGAGA+TGG + Chr5:90873026-90873045 MsG0580029047.01.T01:intron 45.0%
AGAAGAATATCGTCGGAGGT+GGG - Chr5:90871931-90871950 None:intergenic 45.0%
ATCGGAAATCACTTGTGCTC+AGG + Chr5:90871963-90871982 MsG0580029047.01.T01:CDS 45.0%
CCAAGTCAAGCACTTTCGTT+AGG + Chr5:90871770-90871789 MsG0580029047.01.T01:CDS 45.0%
CTACCCGGAAAAAACTCTTG+AGG - Chr5:90874026-90874045 None:intergenic 45.0%
CTCTCAACAGAACAGGTCAA+GGG - Chr5:90873624-90873643 None:intergenic 45.0%
GAAAGAATTTGGCGGGAGAT+GGG + Chr5:90873027-90873046 MsG0580029047.01.T01:intron 45.0%
GCTCAGGAAACCGATGATTT+CGG + Chr5:90871979-90871998 MsG0580029047.01.T01:CDS 45.0%
GTTAGGAGGATGTGGTACTT+TGG + Chr5:90873257-90873276 MsG0580029047.01.T01:intron 45.0%
GTTTCGTTCGCCTTCCAATT+TGG + Chr5:90873355-90873374 MsG0580029047.01.T01:intron 45.0%
TATGCTCAGAATCAGGCACA+AGG - Chr5:90874215-90874234 None:intergenic 45.0%
TCTCTCAACAGAACAGGTCA+AGG - Chr5:90873625-90873644 None:intergenic 45.0%
TCTTCACCAGTAGCCAAATC+AGG - Chr5:90874481-90874500 None:intergenic 45.0%
TCTTCTTCCGGAATTCTACC+CGG - Chr5:90874041-90874060 None:intergenic 45.0%
TGAAAATCTGGCCCGTCATA+AGG - Chr5:90874691-90874710 None:intergenic 45.0%
TGAGGTTCACATGCCTGATT+TGG + Chr5:90874465-90874484 MsG0580029047.01.T01:CDS 45.0%
TTATCTTTCGTGAGAGGGGT+TGG + Chr5:90873213-90873232 MsG0580029047.01.T01:intron 45.0%
! ACTTACAGCGCCGAAATCAT+CGG - Chr5:90871992-90872011 None:intergenic 45.0%
! ATTGGGTTGTGTTGCTAACC+TGG + Chr5:90874950-90874969 MsG0580029047.01.T01:intron 45.0%
! CAGCTAGTTAGTCTCAGGTT+TGG + Chr5:90873992-90874011 MsG0580029047.01.T01:intron 45.0%
! TGCAGTTCAGATTGCGGTTT+TGG + Chr5:90875018-90875037 MsG0580029047.01.T01:intron 45.0%
! TGTAGCAGCTAGTTAGTCTC+AGG + Chr5:90873987-90874006 MsG0580029047.01.T01:intron 45.0%
!! CCTAACGAAAGTGCTTGACT+TGG - Chr5:90871773-90871792 None:intergenic 45.0%
!! GGTTTTGTGTTAGGAGGATG+TGG + Chr5:90873249-90873268 MsG0580029047.01.T01:intron 45.0%
!! GTTGTACAATCTGGTTTGGG+TGG + Chr5:90874076-90874095 MsG0580029047.01.T01:intron 45.0%
!! GTTTTGAGCTTGGCATGGTT+AGG + Chr5:90874106-90874125 MsG0580029047.01.T01:intron 45.0%
!! TCAAGTTTCTATTGGTGGCG+TGG + Chr5:90873937-90873956 MsG0580029047.01.T01:intron 45.0%
!! TGAACCCTGGTATCGATTGA+CGG - Chr5:90871863-90871882 None:intergenic 45.0%
!!! GCACTCTGGAGTTTTTCAAG+GGG + Chr5:90873716-90873735 MsG0580029047.01.T01:intron 45.0%
!!! TGGTGTGTCAGTTTTGAGCT+TGG + Chr5:90874096-90874115 MsG0580029047.01.T01:intron 45.0%
!!! TGTCAGTTTTGAGCTTGGCA+TGG + Chr5:90874101-90874120 MsG0580029047.01.T01:intron 45.0%
!!! TTTTTGGTCTTGAGCACCGA+TGG + Chr5:90873080-90873099 MsG0580029047.01.T01:intron 45.0%
!! AAAAAAAAAAAAAATTGAAA+AGG - Chr5:90874251-90874270 None:intergenic 5.0%
!!! TTTAATTTCATTTATATATA+GGG + Chr5:90872071-90872090 MsG0580029047.01.T01:intron 5.0%
!!! TTTTAATTTCATTTATATAT+AGG + Chr5:90872070-90872089 MsG0580029047.01.T01:intron 5.0%
AAGAATTTGGCGGGAGATGG+GGG + Chr5:90873029-90873048 MsG0580029047.01.T01:intron 50.0%
ACGGAGAAGAATATCGTCGG+AGG - Chr5:90871935-90871954 None:intergenic 50.0%
GAGAAGAATATCGTCGGAGG+TGG - Chr5:90871932-90871951 None:intergenic 50.0%
GGGTAGGGCTGTGGATTTAT+GGG + Chr5:90873119-90873138 MsG0580029047.01.T01:intron 50.0%
TAAGAAGGATGGGCCCTCTT+TGG + Chr5:90874582-90874601 MsG0580029047.01.T01:CDS 50.0%
TCTCTACAACCAAGCAGTCC+AGG - Chr5:90874971-90874990 None:intergenic 50.0%
TGGGTAGGGCTGTGGATTTA+TGG + Chr5:90873118-90873137 MsG0580029047.01.T01:intron 50.0%
TGTTGCTAACCTGGACTGCT+TGG + Chr5:90874959-90874978 MsG0580029047.01.T01:intron 50.0%
TTTCTCCTGCTGCAGTAGTG+AGG + Chr5:90873793-90873812 MsG0580029047.01.T01:intron 50.0%
! TATGTACTTGTTGAGGGGGG+TGG + Chr5:90873680-90873699 MsG0580029047.01.T01:intron 50.0%
!! CACATGCCTGATTTGGCTAC+TGG + Chr5:90874472-90874491 MsG0580029047.01.T01:CDS 50.0%
!! CTTGAGCACCGATGGTAATG+AGG + Chr5:90873088-90873107 MsG0580029047.01.T01:intron 50.0%
!! GTGTTGCTTCAGTTGCACTC+TGG + Chr5:90873702-90873721 MsG0580029047.01.T01:intron 50.0%
GAGGTCTGAGTCTGACTGCT+TGG + Chr5:90873812-90873831 MsG0580029047.01.T01:intron 55.0%
GTGTAGATTGGGTAGGGCTG+TGG + Chr5:90873110-90873129 MsG0580029047.01.T01:intron 55.0%
TCAGACCTCACTACTGCAGC+AGG - Chr5:90873801-90873820 None:intergenic 55.0%
!! ACCCTGGTATCGATTGACGG+CGG - Chr5:90871860-90871879 None:intergenic 55.0%
!! GTTTCTATTGGTGGCGTGGC+AGG + Chr5:90873941-90873960 MsG0580029047.01.T01:intron 55.0%
ATTGACGGCGGCGGAAAGCA+CGG - Chr5:90871848-90871867 None:intergenic 60.0%
GCCGCCGTCAATCGATACCA+GGG + Chr5:90871856-90871875 MsG0580029047.01.T01:CDS 60.0%
!! CTGGTATCGATTGACGGCGG+CGG - Chr5:90871857-90871876 None:intergenic 60.0%
CGCCGCCGTCAATCGATACC+AGG + Chr5:90871855-90871874 MsG0580029047.01.T01:CDS 65.0%
! GAAAGCACGGCGCGATGACC+CGG - Chr5:90871835-90871854 None:intergenic 65.0%
CACGGCGCGATGACCCGGTT+GGG - Chr5:90871830-90871849 None:intergenic 70.0%
GCACGGCGCGATGACCCGGT+TGG - Chr5:90871831-90871850 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr5 gene 90871745 90875286 90871745 ID=MsG0580029047.01;Name=MsG0580029047.01
Chr5 mRNA 90871745 90875286 90871745 ID=MsG0580029047.01.T01;Parent=MsG0580029047.01;Name=MsG0580029047.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|1|1|3|0|255
Chr5 exon 90871745 90872005 90871745 ID=MsG0580029047.01.T01:exon:26169;Parent=MsG0580029047.01.T01
Chr5 exon 90874364 90874710 90874364 ID=MsG0580029047.01.T01:exon:26170;Parent=MsG0580029047.01.T01
Chr5 exon 90875127 90875286 90875127 ID=MsG0580029047.01.T01:exon:26171;Parent=MsG0580029047.01.T01
Chr5 CDS 90871745 90872005 90871745 ID=MsG0580029047.01.T01:cds;Parent=MsG0580029047.01.T01
Chr5 CDS 90874364 90874710 90874364 ID=MsG0580029047.01.T01:cds;Parent=MsG0580029047.01.T01
Chr5 CDS 90875127 90875286 90875127 ID=MsG0580029047.01.T01:cds;Parent=MsG0580029047.01.T01
Gene Sequence

>MsG0580029047.01.T01

ATGGCGTTCAATTCAATTCTTCGTTCCAAGTCAAGCACTTTCGTTAGGGCTTTTTCTTCTGCGACTCAATTGATCAGAAAAACCCAACCGGGTCATCGCGCCGTGCTTTCCGCCGCCGTCAATCGATACCAGGGTTCATTTGTTCCTACGTTTCACTTTTCTTCTGTATCTGTCAAAAACAAACCCACCTCCGACGATATTCTTCTCCGTTTCATCGAATCGGAAATCACTTGTGCTCAGGAAACCGATGATTTCGGCGCTGGTGAGGAAGTTCCAAGCAATTTCCCATTTAAGATAGTTGATAATCCAGGAAATCAGACAATAACACTTAAAAGGACTTATCAAGGTGAAGAAATTCAAGTTGAGGTTCACATGCCTGATTTGGCTACTGGTGAAGAACATGATCATCAAGATGATGATAAGGATGATGAGAGTGAGAGAGCAACTCAATCAAGCATTCCACTTTCAGTCAGTGTTTCTAAGAAGGATGGGCCCTCTTTGGAGTTCAGTTGTGTTGCTTACCCAGATGAGATTGTTATCGACAGCTTGTCTGTAAAGAATCCTGATTTTTCTGATGAACAGATTCCTTATGACGGGCCAGATTTTCAGGATTTGGATGAGGCATTGCAAAAGTCATTCCACAAGTACTTAGAAATAAGGGGAATCAAACCAAGCACAACAAATTTCTTGCATGAGTACATGATCAACAAGGATAGTAGAGAATACTTGGTGTGGTTGAATAAGCTCAAGAATTTCATTAAAGCATGA
Protein sequence

>MsG0580029047.01.T01

MAFNSILRSKSSTFVRAFSSATQLIRKTQPGHRAVLSAAVNRYQGSFVPTFHFSSVSVKNKPTSDDILLRFIESEITCAQETDDFGAGEEVPSNFPFKIVDNPGNQTITLKRTYQGEEIQVEVHMPDLATGEEHDHQDDDKDDESERATQSSIPLSVSVSKKDGPSLEFSCVAYPDEIVIDSLSVKNPDFSDEQIPYDGPDFQDLDEALQKSFHKYLEIRGIKPSTTNFLHEYMINKDSREYLVWLNKLKNFIKA*