Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005789.01.T01 | XP_013470020.1 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 1.20E-60 | 190 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005789.01.T01 | A0A072VRJ2 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 5.74e-61 | 190 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000118.01 | MsG0180005789.01 | 0.814361 | 1.602140e-51 | 1.072754e-48 |
| MsG0180003585.01 | MsG0180005789.01 | 0.861569 | 9.354761e-64 | 2.658783e-60 |
| MsG0180003897.01 | MsG0180005789.01 | 0.844986 | 5.380918e-59 | 8.838949e-56 |
| MsG0180005789.01 | MsG0280006629.01 | 0.818860 | 1.572409e-52 | 1.190861e-49 |
| MsG0180005789.01 | MsG0280006747.01 | 0.832546 | 8.885894e-56 | 9.964248e-53 |
| MsG0180005789.01 | MsG0280006760.01 | 0.845691 | 3.467171e-59 | 5.825915e-56 |
| MsG0180005789.01 | MsG0280007055.01 | 0.829017 | 6.512381e-55 | 6.582801e-52 |
| MsG0180005789.01 | MsG0280007606.01 | 0.847103 | 1.428834e-59 | 2.512865e-56 |
| MsG0180005789.01 | MsG0280010792.01 | 0.858724 | 6.767389e-63 | 1.743941e-59 |
| MsG0180005789.01 | MsG0380014507.01 | 0.871698 | 5.605503e-67 | 2.282584e-63 |
| MsG0180005789.01 | MsG0380015446.01 | 0.804054 | 2.597187e-49 | 1.326017e-46 |
| MsG0180005789.01 | MsG0380015857.01 | 0.830472 | 2.880932e-55 | 3.038130e-52 |
| MsG0180005789.01 | MsG0380016744.01 | 0.849683 | 2.763523e-60 | 5.280965e-57 |
| MsG0180005789.01 | MsG0380016760.01 | 0.822224 | 2.656074e-53 | 2.209530e-50 |
| MsG0180005789.01 | MsG0380017664.01 | 0.856868 | 2.404471e-62 | 5.820528e-59 |
| MsG0180005789.01 | MsG0480018164.01 | 0.811382 | 7.198033e-51 | 4.449022e-48 |
| MsG0180005789.01 | MsG0480020796.01 | 0.859867 | 3.073613e-63 | 8.238743e-60 |
| MsG0180005789.01 | MsG0480020911.01 | 0.812380 | 4.365111e-51 | 2.771133e-48 |
| MsG0180005789.01 | MsG0480021279.01 | 0.855622 | 5.575020e-62 | 1.294947e-58 |
| MsG0180005789.01 | MsG0480021288.01 | 0.803642 | 3.162680e-49 | 1.597658e-46 |
| MsG0180005789.01 | MsG0480021417.01 | 0.831427 | 1.679955e-55 | 1.822350e-52 |
| MsG0180005789.01 | MsG0480022086.01 | 0.821510 | 3.886250e-53 | 3.167906e-50 |
| MsG0180005789.01 | MsG0480023931.01 | 0.833254 | 5.925446e-56 | 6.786439e-53 |
| MsG0180005789.01 | MsG0480023996.01 | 0.807671 | 4.510901e-50 | 2.527688e-47 |
| MsG0180005789.01 | MsG0580024540.01 | 0.846900 | 1.623732e-59 | 2.836542e-56 |
| MsG0180005789.01 | MsG0580024738.01 | 0.821558 | 3.788705e-53 | 3.092656e-50 |
| MsG0180005789.01 | MsG0580024894.01 | 0.804462 | 2.135675e-49 | 1.101808e-46 |
| MsG0180005789.01 | MsG0580029047.01 | 0.806086 | 9.755031e-50 | 5.247538e-47 |
| MsG0180005789.01 | MsG0680031368.01 | 0.824564 | 7.539001e-54 | 6.702491e-51 |
| MsG0180005789.01 | MsG0680032459.01 | 0.814864 | 1.240098e-51 | 8.416687e-49 |
| MsG0180005789.01 | MsG0780038383.01 | 0.803452 | 3.463318e-49 | 1.741008e-46 |
| MsG0180005789.01 | MsG0780038861.01 | 0.825875 | 3.692479e-54 | 3.407974e-51 |
| MsG0180005789.01 | MsG0780040174.01 | 0.824228 | 9.044973e-54 | 7.963589e-51 |
| MsG0180005789.01 | MsG0780041790.01 | 0.834320 | 3.209307e-56 | 3.795454e-53 |
| MsG0180005789.01 | MsG0880042517.01 | 0.840970 | 6.309453e-58 | 9.136519e-55 |
| MsG0180005789.01 | MsG0880042896.01 | 0.866220 | 3.342153e-65 | 1.117691e-61 |
| MsG0180005789.01 | MsG0880043076.01 | 0.851384 | 9.191975e-61 | 1.857365e-57 |
| MsG0180005789.01 | MsG0880046486.01 | 0.814682 | 1.360327e-51 | 9.187990e-49 |
| MsG0180005789.01 | MsG0880046760.01 | 0.813165 | 2.939094e-51 | 1.904959e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005789.01.T01 | MTR_1g107085 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 1.46e-64 | 190 |
| MsG0180005789.01.T01 | MTR_1g107085 | 92.784 | 97 | 7 | 0 | 1 | 97 | 1 | 97 | 1.06e-47 | 147 |
| MsG0180005789.01.T01 | MTR_1g107085 | 95.745 | 94 | 4 | 0 | 1 | 94 | 1 | 94 | 3.02e-47 | 146 |
| MsG0180005789.01.T01 | MTR_1g107085 | 94.737 | 95 | 5 | 0 | 1 | 95 | 1 | 95 | 7.48e-47 | 145 |
| MsG0180005789.01.T01 | MTR_5g016330 | 48.454 | 97 | 37 | 4 | 1 | 93 | 1 | 88 | 2.80e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005789.01.T01 | AT2G15000 | 55.238 | 105 | 41 | 4 | 1 | 103 | 1 | 101 | 3.58e-24 | 88.6 |
| MsG0180005789.01.T01 | AT2G15000 | 55.238 | 105 | 41 | 4 | 1 | 103 | 1 | 101 | 3.58e-24 | 88.6 |
| MsG0180005789.01.T01 | AT2G15000 | 55.238 | 105 | 41 | 4 | 1 | 103 | 1 | 101 | 3.58e-24 | 88.6 |
| MsG0180005789.01.T01 | AT2G15000 | 55.238 | 105 | 41 | 4 | 1 | 103 | 1 | 101 | 3.58e-24 | 88.6 |
| MsG0180005789.01.T01 | AT2G15000 | 53.608 | 97 | 39 | 4 | 1 | 95 | 1 | 93 | 3.93e-18 | 72.8 |
| MsG0180005789.01.T01 | AT4G34265 | 46.491 | 114 | 34 | 5 | 1 | 103 | 1 | 98 | 1.07e-17 | 72.0 |
| MsG0180005789.01.T01 | AT4G34265 | 46.491 | 114 | 34 | 5 | 1 | 103 | 1 | 98 | 1.07e-17 | 72.0 |
| MsG0180005789.01.T01 | AT4G34265 | 46.491 | 114 | 34 | 5 | 1 | 103 | 1 | 98 | 1.07e-17 | 72.0 |
| MsG0180005789.01.T01 | AT2G15000 | 53.125 | 96 | 39 | 4 | 1 | 94 | 1 | 92 | 1.81e-17 | 71.2 |
| MsG0180005789.01.T01 | AT2G15000 | 53.125 | 96 | 39 | 4 | 1 | 94 | 1 | 92 | 1.81e-17 | 71.2 |
| MsG0180005789.01.T01 | AT4G34265 | 44.340 | 106 | 32 | 5 | 1 | 95 | 1 | 90 | 9.75e-13 | 59.3 |
| MsG0180005789.01.T01 | AT4G34265 | 43.396 | 106 | 33 | 5 | 1 | 95 | 1 | 90 | 4.06e-12 | 57.8 |
| MsG0180005789.01.T01 | AT4G34265 | 43.396 | 106 | 33 | 5 | 1 | 95 | 1 | 90 | 4.06e-12 | 57.8 |
Find 43 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATGCCTGCTACCTCTTCC+AGG | 0.234541 | 1:-96153309 | MsG0180005789.01.T01:intron |
| GTGCAGGAATTTGGGGCAAT+TGG | 0.247985 | 1:-96153193 | MsG0180005789.01.T01:intron |
| TGCAGGAATTTGGGGCAATT+GGG | 0.381549 | 1:-96153192 | MsG0180005789.01.T01:intron |
| CGGAGGCGAGGAAGGGAAGA+AGG | 0.397485 | 1:+96153376 | None:intergenic |
| ATAACAATGGCGTTGCGATC+TGG | 0.400559 | 1:-96153454 | None:intergenic |
| TGGCGAGTGGCGGAGGACGG+AGG | 0.410736 | 1:+96153359 | None:intergenic |
| CGAGGGGTGGCGAGTGGCGG+AGG | 0.419804 | 1:+96153352 | None:intergenic |
| GCGGAGGACGGAGGCGAGGA+AGG | 0.437115 | 1:+96153368 | None:intergenic |
| AGAAGGAGCCGCCGGACGAA+GGG | 0.456281 | 1:+96153393 | None:intergenic |
| AAGAGGCGGGGCTGAGTGCG+AGG | 0.481512 | 1:+96153334 | None:intergenic |
| GGCCAGGTCTTCCCTTCGTC+CGG | 0.483656 | 1:-96153404 | MsG0180005789.01.T01:CDS |
| AGCAGGCATGGAAAAGAGGC+GGG | 0.485396 | 1:+96153321 | None:intergenic |
| AGACCTGGCCGTGGAGATGA+AGG | 0.492112 | 1:+96153417 | None:intergenic |
| GCTCCTTCATCTCCACGGCC+AGG | 0.498839 | 1:-96153420 | MsG0180005789.01.T01:CDS |
| CGGAGGACGGAGGCGAGGAA+GGG | 0.500771 | 1:+96153369 | None:intergenic |
| GTGCGAGGGGTGGCGAGTGG+CGG | 0.502077 | 1:+96153349 | None:intergenic |
| CCTGGAAGAGGTAGCAGGCA+TGG | 0.513058 | 1:+96153309 | None:intergenic |
| CGCCGGACGAAGGGAAGACC+TGG | 0.513153 | 1:+96153402 | None:intergenic |
| GGGTGGCGAGTGGCGGAGGA+CGG | 0.517554 | 1:+96153356 | None:intergenic |
| TTAGGGTTTGGACCTGGAAG+AGG | 0.524458 | 1:+96153297 | None:intergenic |
| TTGGACCTGGAAGAGGTAGC+AGG | 0.529380 | 1:+96153304 | None:intergenic |
| AGGTAGCAGGCATGGAAAAG+AGG | 0.531493 | 1:+96153317 | None:intergenic |
| AGTGGCGGAGGACGGAGGCG+AGG | 0.533816 | 1:+96153364 | None:intergenic |
| ACGAAGGGAAGACCTGGCCG+TGG | 0.538799 | 1:+96153408 | None:intergenic |
| AGAGGCGGGGCTGAGTGCGA+GGG | 0.539518 | 1:+96153335 | None:intergenic |
| AAGAAGGAGCCGCCGGACGA+AGG | 0.539975 | 1:+96153392 | None:intergenic |
| ATCTCGCTCCTTCATCTCCA+CGG | 0.540316 | 1:-96153425 | MsG0180005789.01.T01:CDS |
| AAGCTCGTGCGCTGACGGCA+AGG | 0.543327 | 1:+96153109 | None:intergenic |
| GAGGCGGGGCTGAGTGCGAG+GGG | 0.547076 | 1:+96153336 | None:intergenic |
| GGAAGGGAAGAAGGAGCCGC+CGG | 0.548993 | 1:+96153385 | None:intergenic |
| GCGGGGCTGAGTGCGAGGGG+TGG | 0.550387 | 1:+96153339 | None:intergenic |
| TGAGTGCGAGGGGTGGCGAG+TGG | 0.550653 | 1:+96153346 | None:intergenic |
| GCTACAAGCTCGTGCGCTGA+CGG | 0.567501 | 1:+96153104 | None:intergenic |
| AGAACGACGGAAGGTACCAT+TGG | 0.577356 | 1:+96153074 | None:intergenic |
| TAGCAGGCATGGAAAAGAGG+CGG | 0.583179 | 1:+96153320 | None:intergenic |
| TTTGTTGGTGCAGGAATTTG+GGG | 0.587970 | 1:-96153200 | MsG0180005789.01.T01:intron |
| GCTTGTAGCGAGCTGTCCAA+TGG | 0.595505 | 1:-96153090 | MsG0180005789.01.T01:CDS |
| ACAGCCATCGTGCTATGCAG+TGG | 0.621490 | 1:+96153150 | None:intergenic |
| ATCTTGACAAGAACGACGGA+AGG | 0.626544 | 1:+96153065 | None:intergenic |
| CAGGTCTTCCCTTCGTCCGG+CGG | 0.627826 | 1:-96153401 | MsG0180005789.01.T01:CDS |
| GCAGGCATGGAAAAGAGGCG+GGG | 0.645770 | 1:+96153322 | None:intergenic |
| TTTGCCACTGCATAGCACGA+TGG | 0.666045 | 1:-96153154 | MsG0180005789.01.T01:CDS |
| AGCGATCTTGACAAGAACGA+CGG | 0.707528 | 1:+96153061 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTGAATGTGTTTTTTTTGT+TGG | - | Chr1:96153292-96153311 | MsG0180005789.01.T01:intron | 25.0% |
| ATTGTCGTTGAGCTTGAATT+GGG | + | Chr1:96153238-96153257 | None:intergenic | 35.0% | |
| TGAGCTTGAATTGGGAATTA+GGG | + | Chr1:96153230-96153249 | None:intergenic | 35.0% | |
| TTGAATTGGGAATTAGGGTT+TGG | + | Chr1:96153225-96153244 | None:intergenic | 35.0% | |
| TTGAGCTTGAATTGGGAATT+AGG | + | Chr1:96153231-96153250 | None:intergenic | 35.0% | |
| ! | TTTTGTTGGTGCAGGAATTT+GGG | - | Chr1:96153306-96153325 | MsG0180005789.01.T01:intron | 35.0% |
| ! | TTTTTGTTGGTGCAGGAATT+TGG | - | Chr1:96153305-96153324 | MsG0180005789.01.T01:intron | 35.0% |
| !!! | TGTGTTTTTTTTGTTGGTGC+AGG | - | Chr1:96153298-96153317 | MsG0180005789.01.T01:intron | 35.0% |
| CATTGTCGTTGAGCTTGAAT+TGG | + | Chr1:96153239-96153258 | None:intergenic | 40.0% | |
| TTTGTTGGTGCAGGAATTTG+GGG | - | Chr1:96153307-96153326 | MsG0180005789.01.T01:intron | 40.0% | |
| AGCGATCTTGACAAGAACGA+CGG | + | Chr1:96153449-96153468 | MsG0180005788.01.T01:CDS | 45.0% | |
| ATCTTGACAAGAACGACGGA+AGG | + | Chr1:96153445-96153464 | MsG0180005788.01.T01:CDS | 45.0% | |
| ! | TGCAGGAATTTGGGGCAATT+GGG | - | Chr1:96153315-96153334 | MsG0180005789.01.T01:CDS | 45.0% |
| AGGTAGCAGGCATGGAAAAG+AGG | + | Chr1:96153193-96153212 | None:intergenic | 50.0% | |
| ATCTCGCTCCTTCATCTCCA+CGG | - | Chr1:96153082-96153101 | MsG0180005789.01.T01:CDS | 50.0% | |
| TAGCAGGCATGGAAAAGAGG+CGG | + | Chr1:96153190-96153209 | None:intergenic | 50.0% | |
| TGGGAATTAGGGTTTGGACC+TGG | + | Chr1:96153219-96153238 | None:intergenic | 50.0% | |
| TTAGGGTTTGGACCTGGAAG+AGG | + | Chr1:96153213-96153232 | None:intergenic | 50.0% | |
| TTTGCCACTGCATAGCACGA+TGG | - | Chr1:96153353-96153372 | MsG0180005789.01.T01:CDS | 50.0% | |
| ! | GTGCAGGAATTTGGGGCAAT+TGG | - | Chr1:96153314-96153333 | MsG0180005789.01.T01:CDS | 50.0% |
| !! | AGAACGACGGAAGGTACCAT+TGG | + | Chr1:96153436-96153455 | MsG0180005788.01.T01:CDS | 50.0% |
| ACAGCCATCGTGCTATGCAG+TGG | + | Chr1:96153360-96153379 | None:intergenic | 55.0% | |
| AGCAGGCATGGAAAAGAGGC+GGG | + | Chr1:96153189-96153208 | None:intergenic | 55.0% | |
| GCTTGTAGCGAGCTGTCCAA+TGG | - | Chr1:96153417-96153436 | MsG0180005789.01.T01:CDS | 55.0% | |
| TTGGACCTGGAAGAGGTAGC+AGG | + | Chr1:96153206-96153225 | None:intergenic | 55.0% | |
| AGACCTGGCCGTGGAGATGA+AGG | + | Chr1:96153093-96153112 | None:intergenic | 60.0% | |
| CCATGCCTGCTACCTCTTCC+AGG | - | Chr1:96153198-96153217 | MsG0180005789.01.T01:intron | 60.0% | |
| CCTGGAAGAGGTAGCAGGCA+TGG | + | Chr1:96153201-96153220 | None:intergenic | 60.0% | |
| GCAGGCATGGAAAAGAGGCG+GGG | + | Chr1:96153188-96153207 | None:intergenic | 60.0% | |
| GCTACAAGCTCGTGCGCTGA+CGG | + | Chr1:96153406-96153425 | None:intergenic | 60.0% | |
| AAGAAGGAGCCGCCGGACGA+AGG | + | Chr1:96153118-96153137 | None:intergenic | 65.0% | |
| ACGAAGGGAAGACCTGGCCG+TGG | + | Chr1:96153102-96153121 | None:intergenic | 65.0% | |
| AGAAGGAGCCGCCGGACGAA+GGG | + | Chr1:96153117-96153136 | None:intergenic | 65.0% | |
| CAGGTCTTCCCTTCGTCCGG+CGG | - | Chr1:96153106-96153125 | MsG0180005789.01.T01:CDS | 65.0% | |
| CGGAGGCGAGGAAGGGAAGA+AGG | + | Chr1:96153134-96153153 | None:intergenic | 65.0% | |
| GCTCCTTCATCTCCACGGCC+AGG | - | Chr1:96153087-96153106 | MsG0180005789.01.T01:CDS | 65.0% | |
| GGAAGGGAAGAAGGAGCCGC+CGG | + | Chr1:96153125-96153144 | None:intergenic | 65.0% | |
| GGCCAGGTCTTCCCTTCGTC+CGG | - | Chr1:96153103-96153122 | MsG0180005789.01.T01:CDS | 65.0% | |
| ! | AAGCTCGTGCGCTGACGGCA+AGG | + | Chr1:96153401-96153420 | None:intergenic | 65.0% |
| AAGAGGCGGGGCTGAGTGCG+AGG | + | Chr1:96153176-96153195 | None:intergenic | 70.0% | |
| AGAGGCGGGGCTGAGTGCGA+GGG | + | Chr1:96153175-96153194 | None:intergenic | 70.0% | |
| CGCCGGACGAAGGGAAGACC+TGG | + | Chr1:96153108-96153127 | None:intergenic | 70.0% | |
| CGGAGGACGGAGGCGAGGAA+GGG | + | Chr1:96153141-96153160 | None:intergenic | 70.0% | |
| TGAGTGCGAGGGGTGGCGAG+TGG | + | Chr1:96153164-96153183 | None:intergenic | 70.0% | |
| GAGGCGGGGCTGAGTGCGAG+GGG | + | Chr1:96153174-96153193 | None:intergenic | 75.0% | |
| GCGGAGGACGGAGGCGAGGA+AGG | + | Chr1:96153142-96153161 | None:intergenic | 75.0% | |
| GTGCGAGGGGTGGCGAGTGG+CGG | + | Chr1:96153161-96153180 | None:intergenic | 75.0% | |
| ! | AGTGGCGGAGGACGGAGGCG+AGG | + | Chr1:96153146-96153165 | None:intergenic | 75.0% |
| ! | GGGTGGCGAGTGGCGGAGGA+CGG | + | Chr1:96153154-96153173 | None:intergenic | 75.0% |
| ! | TGGCGAGTGGCGGAGGACGG+AGG | + | Chr1:96153151-96153170 | None:intergenic | 75.0% |
| ! | CGAGGGGTGGCGAGTGGCGG+AGG | + | Chr1:96153158-96153177 | None:intergenic | 80.0% |
| ! | GCGGGGCTGAGTGCGAGGGG+TGG | + | Chr1:96153171-96153190 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 96153059 | 96153470 | 96153059 | ID=MsG0180005789.01;Name=MsG0180005789.01 |
| Chr1 | mRNA | 96153059 | 96153470 | 96153059 | ID=MsG0180005789.01.T01;Parent=MsG0180005789.01;Name=MsG0180005789.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|103 |
| Chr1 | exon | 96153059 | 96153209 | 96153059 | ID=MsG0180005789.01.T01:exon:40850;Parent=MsG0180005789.01.T01 |
| Chr1 | exon | 96153310 | 96153470 | 96153310 | ID=MsG0180005789.01.T01:exon:40849;Parent=MsG0180005789.01.T01 |
| Chr1 | CDS | 96153310 | 96153470 | 96153310 | ID=MsG0180005789.01.T01:cds;Parent=MsG0180005789.01.T01 |
| Chr1 | CDS | 96153059 | 96153209 | 96153059 | ID=MsG0180005789.01.T01:cds;Parent=MsG0180005789.01.T01 |
| Gene Sequence |
| Protein sequence |
