AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003585.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003585.01.T01 MTR_1g064500 100.000 166 0 0 1 166 1 166 1.22e-116 327
MsG0180003585.01.T01 MTR_4g059400 99.390 164 1 0 1 164 1 164 1.26e-114 322
MsG0180003585.01.T01 MTR_2g094300 83.537 164 27 0 1 164 1 164 1.27e-97 279
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003585.01.T01 AT5G60670 90.964 166 15 0 1 166 1 166 1.32e-107 304
MsG0180003585.01.T01 AT2G37190 90.854 164 15 0 1 164 1 164 6.00e-107 303
MsG0180003585.01.T01 AT3G53430 90.244 164 16 0 1 164 1 164 1.15e-106 302

Find 56 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCTTCGCTTGACGATTC+TGG 0.204840 1:-64732118 None:intergenic
ATGTCTTCTCCGATCTTCTT+TGG 0.275399 1:-64732038 None:intergenic
ACGAAGAATATTAAGCATAA+TGG 0.343409 1:+64732209 MsG0180003585.01.T01:CDS
ATCTGTGATGGGTCGAGCTT+TGG 0.352336 1:-64731930 None:intergenic
CTTGGAACTTGTGTTTCTGT+TGG 0.355041 1:+64732323 MsG0180003585.01.T01:CDS
CGAAGAATATTAAGCATAAT+GGG 0.358983 1:+64732210 MsG0180003585.01.T01:CDS
TCTGTTGGGTGTACTGTTGA+TGG 0.361476 1:+64732338 MsG0180003585.01.T01:CDS
CGCGGCGGCGCTTGTGATCA+AGG 0.362603 1:+64732154 MsG0180003585.01.T01:CDS
AATCTCTTGTTGCAAATCCT+TGG 0.364185 1:-64732369 None:intergenic
ATCTCTTGTTGCAAATCCTT+GGG 0.391437 1:-64732368 None:intergenic
TTGGAACTTGTGTTTCTGTT+GGG 0.400252 1:+64732324 MsG0180003585.01.T01:CDS
TGAAGGAACCAGAGAGAGAT+AGG 0.404063 1:+64732180 MsG0180003585.01.T01:CDS
GTCTTCTTCCTATCTCTCTC+TGG 0.414689 1:-64732188 None:intergenic
GAGACCGAGGGGACCGATCT+TGG 0.416952 1:-64732012 None:intergenic
CCAAAGAAACCGCAAAAGAC+TGG 0.428144 1:+64732063 MsG0180003585.01.T01:CDS
AGACCGAGGGGACCGATCTT+GGG 0.428565 1:-64732011 None:intergenic
GATCTTGGGAGCGAGGGAAC+TGG 0.450092 1:-64731997 None:intergenic
GGTACTGTGAAGGAGATTCT+TGG 0.451833 1:+64732305 MsG0180003585.01.T01:CDS
TGTGATGGGTCGAGCTTTGG+AGG 0.459117 1:-64731927 None:intergenic
TCCATGGCGAAGGAGCTTGC+GGG 0.471935 1:+64732284 MsG0180003585.01.T01:CDS
AGCTCCTTCGCCATGGATCT+CGG 0.474540 1:-64732278 None:intergenic
GATTATGAAGCCGAGATCCA+TGG 0.485064 1:+64732268 MsG0180003585.01.T01:CDS
GAAGTCTACGTCCGCGTCAC+CGG 0.489324 1:+64731957 MsG0180003585.01.T01:CDS
AGTTCCCTCGCTCCCAAGAT+CGG 0.497799 1:+64731999 MsG0180003585.01.T01:CDS
CTGTTGGGTGTACTGTTGAT+GGG 0.503488 1:+64732339 MsG0180003585.01.T01:CDS
GGGACCGATCTTGGGAGCGA+GGG 0.509084 1:-64732003 None:intergenic
CGCGTCACCGGTGGAGAAGT+CGG 0.509186 1:+64731969 MsG0180003585.01.T01:CDS
GGGGACCGATCTTGGGAGCG+AGG 0.533033 1:-64732004 None:intergenic
ATCCATGGCGAAGGAGCTTG+CGG 0.534410 1:+64732283 MsG0180003585.01.T01:CDS
GCTCCCAAGATCGGTCCCCT+CGG 0.536064 1:+64732008 MsG0180003585.01.T01:CDS
GCTTGTGATCAAGGCGTTGA+AGG 0.539531 1:+64732163 MsG0180003585.01.T01:CDS
ATCACAAGCGCCGCCGCGGA+GGG 0.546925 1:-64732149 None:intergenic
GGCGGCGCCGACTTCTCCAC+CGG 0.558047 1:-64731976 None:intergenic
GTCTACGTCCGCGTCACCGG+TGG 0.568502 1:+64731960 MsG0180003585.01.T01:CDS
TTGCAACAAGAGATTGATGA+TGG 0.571171 1:+64732377 MsG0180003585.01.T01:CDS
ACCCGCAAGCTCCTTCGCCA+TGG 0.574079 1:-64732285 None:intergenic
CTTGGGAGCGAGGGAACTGG+CGG 0.575994 1:-64731994 None:intergenic
GGTCTCTCTCCAAAGAAGAT+CGG 0.584278 1:+64732029 MsG0180003585.01.T01:CDS
GATCACAAGCGCCGCCGCGG+AGG 0.585182 1:-64732150 None:intergenic
TAGACTTCGACGATCTGTGA+TGG 0.587094 1:-64731942 None:intergenic
CGACTTCTCCACCGGTGACG+CGG 0.588865 1:-64731968 None:intergenic
AGAAACCGCAAAAGACTGGA+AGG 0.591239 1:+64732067 MsG0180003585.01.T01:CDS
CTTCTTTGGAGAGAGACCGA+GGG 0.594178 1:-64732024 None:intergenic
GGAGCTTGCGGGTACTGTGA+AGG 0.597173 1:+64732295 MsG0180003585.01.T01:CDS
TGCGGTTGTTCCCTCCGCGG+CGG 0.605465 1:+64732139 MsG0180003585.01.T01:CDS
TCTTCTTTGGAGAGAGACCG+AGG 0.616068 1:-64732025 None:intergenic
AGACTTCGACGATCTGTGAT+GGG 0.618253 1:-64731941 None:intergenic
GAATCGTCAAGCGAAGGTTG+CGG 0.619107 1:+64732121 MsG0180003585.01.T01:CDS
CTTGATCACAAGCGCCGCCG+CGG 0.628883 1:-64732153 None:intergenic
TGTTGATGGGAAAGATCCCA+AGG 0.632585 1:+64732352 MsG0180003585.01.T01:CDS
AGTCCAGAATCGTCAAGCGA+AGG 0.641493 1:+64732115 MsG0180003585.01.T01:CDS
GAAGCCGAGATCCATGGCGA+AGG 0.654215 1:+64732274 MsG0180003585.01.T01:CDS
CTGTGAGCTTGACTGTGACA+CGG 0.663078 1:-64732094 None:intergenic
GAAACCGCAAAAGACTGGAA+GGG 0.670069 1:+64732068 MsG0180003585.01.T01:CDS
GGTTGCGGTTGTTCCCTCCG+CGG 0.676784 1:+64732136 MsG0180003585.01.T01:CDS
TTCTTTGGAGAGAGACCGAG+GGG 0.684696 1:-64732023 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACGAAGAATATTAAGCATAA+TGG + Chr1:64732209-64732228 MsG0180003585.01.T01:CDS 25.0%
! CGAAGAATATTAAGCATAAT+GGG + Chr1:64732210-64732229 MsG0180003585.01.T01:CDS 25.0%
AATCTCTTGTTGCAAATCCT+TGG - Chr1:64732372-64732391 None:intergenic 35.0%
ATCTCTTGTTGCAAATCCTT+GGG - Chr1:64732371-64732390 None:intergenic 35.0%
TTGCAACAAGAGATTGATGA+TGG + Chr1:64732377-64732396 MsG0180003585.01.T01:CDS 35.0%
! TTGGAACTTGTGTTTCTGTT+GGG + Chr1:64732324-64732343 MsG0180003585.01.T01:CDS 35.0%
ATGTCTTCTCCGATCTTCTT+TGG - Chr1:64732041-64732060 None:intergenic 40.0%
! CTTGGAACTTGTGTTTCTGT+TGG + Chr1:64732323-64732342 MsG0180003585.01.T01:CDS 40.0%
AGAAACCGCAAAAGACTGGA+AGG + Chr1:64732067-64732086 MsG0180003585.01.T01:CDS 45.0%
AGACTTCGACGATCTGTGAT+GGG - Chr1:64731944-64731963 None:intergenic 45.0%
CCAAAGAAACCGCAAAAGAC+TGG + Chr1:64732063-64732082 MsG0180003585.01.T01:CDS 45.0%
CTGTTGGGTGTACTGTTGAT+GGG + Chr1:64732339-64732358 MsG0180003585.01.T01:CDS 45.0%
GAAACCGCAAAAGACTGGAA+GGG + Chr1:64732068-64732087 MsG0180003585.01.T01:CDS 45.0%
GATTATGAAGCCGAGATCCA+TGG + Chr1:64732268-64732287 MsG0180003585.01.T01:CDS 45.0%
GGTACTGTGAAGGAGATTCT+TGG + Chr1:64732305-64732324 MsG0180003585.01.T01:CDS 45.0%
GGTCTCTCTCCAAAGAAGAT+CGG + Chr1:64732029-64732048 MsG0180003585.01.T01:CDS 45.0%
GTCTTCTTCCTATCTCTCTC+TGG - Chr1:64732191-64732210 None:intergenic 45.0%
TAGACTTCGACGATCTGTGA+TGG - Chr1:64731945-64731964 None:intergenic 45.0%
TCTGTTGGGTGTACTGTTGA+TGG + Chr1:64732338-64732357 MsG0180003585.01.T01:CDS 45.0%
TGAAGGAACCAGAGAGAGAT+AGG + Chr1:64732180-64732199 MsG0180003585.01.T01:CDS 45.0%
! CCAGTCTTTTGCGGTTTCTT+TGG - Chr1:64732066-64732085 None:intergenic 45.0%
! TGTTGATGGGAAAGATCCCA+AGG + Chr1:64732352-64732371 MsG0180003585.01.T01:CDS 45.0%
AGTCCAGAATCGTCAAGCGA+AGG + Chr1:64732115-64732134 MsG0180003585.01.T01:CDS 50.0%
CAACCTTCGCTTGACGATTC+TGG - Chr1:64732121-64732140 None:intergenic 50.0%
CTGTGAGCTTGACTGTGACA+CGG - Chr1:64732097-64732116 None:intergenic 50.0%
GAATCGTCAAGCGAAGGTTG+CGG + Chr1:64732121-64732140 MsG0180003585.01.T01:CDS 50.0%
! CTTCTTTGGAGAGAGACCGA+GGG - Chr1:64732027-64732046 None:intergenic 50.0%
! GAGACCCTTCCAGTCTTTTG+CGG - Chr1:64732075-64732094 None:intergenic 50.0%
! TCTTCTTTGGAGAGAGACCG+AGG - Chr1:64732028-64732047 None:intergenic 50.0%
! TTCTTTGGAGAGAGACCGAG+GGG - Chr1:64732026-64732045 None:intergenic 50.0%
!! ATCTGTGATGGGTCGAGCTT+TGG - Chr1:64731933-64731952 None:intergenic 50.0%
!! GCTTGTGATCAAGGCGTTGA+AGG + Chr1:64732163-64732182 MsG0180003585.01.T01:CDS 50.0%
AGCTCCTTCGCCATGGATCT+CGG - Chr1:64732281-64732300 None:intergenic 55.0%
AGTTCCCTCGCTCCCAAGAT+CGG + Chr1:64731999-64732018 MsG0180003585.01.T01:CDS 55.0%
ATCCATGGCGAAGGAGCTTG+CGG + Chr1:64732283-64732302 MsG0180003585.01.T01:CDS 55.0%
!! TGTGATGGGTCGAGCTTTGG+AGG - Chr1:64731930-64731949 None:intergenic 55.0%
AGACCGAGGGGACCGATCTT+GGG - Chr1:64732014-64732033 None:intergenic 60.0%
GAAGCCGAGATCCATGGCGA+AGG + Chr1:64732274-64732293 MsG0180003585.01.T01:CDS 60.0%
GAAGTCTACGTCCGCGTCAC+CGG + Chr1:64731957-64731976 MsG0180003585.01.T01:CDS 60.0%
GATCTTGGGAGCGAGGGAAC+TGG - Chr1:64732000-64732019 None:intergenic 60.0%
TCCATGGCGAAGGAGCTTGC+GGG + Chr1:64732284-64732303 MsG0180003585.01.T01:CDS 60.0%
! GGAGCTTGCGGGTACTGTGA+AGG + Chr1:64732295-64732314 MsG0180003585.01.T01:CDS 60.0%
ACCCGCAAGCTCCTTCGCCA+TGG - Chr1:64732288-64732307 None:intergenic 65.0%
CGACTTCTCCACCGGTGACG+CGG - Chr1:64731971-64731990 None:intergenic 65.0%
CGCGTCACCGGTGGAGAAGT+CGG + Chr1:64731969-64731988 MsG0180003585.01.T01:CDS 65.0%
CTTGGGAGCGAGGGAACTGG+CGG - Chr1:64731997-64732016 None:intergenic 65.0%
GAGACCGAGGGGACCGATCT+TGG - Chr1:64732015-64732034 None:intergenic 65.0%
GCTCCCAAGATCGGTCCCCT+CGG + Chr1:64732008-64732027 MsG0180003585.01.T01:CDS 65.0%
GGGACCGATCTTGGGAGCGA+GGG - Chr1:64732006-64732025 None:intergenic 65.0%
GGTTGCGGTTGTTCCCTCCG+CGG + Chr1:64732136-64732155 MsG0180003585.01.T01:CDS 65.0%
! CTTGATCACAAGCGCCGCCG+CGG - Chr1:64732156-64732175 None:intergenic 65.0%
CGCGGCGGCGCTTGTGATCA+AGG + Chr1:64732154-64732173 MsG0180003585.01.T01:CDS 70.0%
GGGGACCGATCTTGGGAGCG+AGG - Chr1:64732007-64732026 None:intergenic 70.0%
GTCTACGTCCGCGTCACCGG+TGG + Chr1:64731960-64731979 MsG0180003585.01.T01:CDS 70.0%
TGCGGTTGTTCCCTCCGCGG+CGG + Chr1:64732139-64732158 MsG0180003585.01.T01:CDS 70.0%
! ATCACAAGCGCCGCCGCGGA+GGG - Chr1:64732152-64732171 None:intergenic 70.0%
! GATCACAAGCGCCGCCGCGG+AGG - Chr1:64732153-64732172 None:intergenic 75.0%
!! GGCGGCGCCGACTTCTCCAC+CGG - Chr1:64731979-64731998 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 64731924 64732424 64731924 ID=MsG0180003585.01;Name=MsG0180003585.01
Chr1 mRNA 64731924 64732424 64731924 ID=MsG0180003585.01.T01;Parent=MsG0180003585.01;Name=MsG0180003585.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|166
Chr1 exon 64731924 64732424 64731924 ID=MsG0180003585.01.T01:exon:50386;Parent=MsG0180003585.01.T01
Chr1 CDS 64731924 64732424 64731924 ID=MsG0180003585.01.T01:cds;Parent=MsG0180003585.01.T01
Gene Sequence

>MsG0180003585.01.T01

ATGCCTCCAAAGCTCGACCCATCACAGATCGTCGAAGTCTACGTCCGCGTCACCGGTGGAGAAGTCGGCGCCGCCAGTTCCCTCGCTCCCAAGATCGGTCCCCTCGGTCTCTCTCCAAAGAAGATCGGAGAAGACATCGCCAAAGAAACCGCAAAAGACTGGAAGGGTCTCCGTGTCACAGTCAAGCTCACAGTCCAGAATCGTCAAGCGAAGGTTGCGGTTGTTCCCTCCGCGGCGGCGCTTGTGATCAAGGCGTTGAAGGAACCAGAGAGAGATAGGAAGAAGACGAAGAATATTAAGCATAATGGGAATATCTCACTTGATGATGTTATTGAGATTGCGAAGATTATGAAGCCGAGATCCATGGCGAAGGAGCTTGCGGGTACTGTGAAGGAGATTCTTGGAACTTGTGTTTCTGTTGGGTGTACTGTTGATGGGAAAGATCCCAAGGATTTGCAACAAGAGATTGATGATGGTGATGTTGAGATTCCTTTAGAATAA

Protein sequence

>MsG0180003585.01.T01

MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGDVEIPLE*