Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023545.01.T01 | XP_013446929.1 | 92.982 | 57 | 3 | 1 | 11 | 66 | 387 | 443 | 4.95E-26 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023545.01.T01 | Q9FLS9 | 59.677 | 62 | 20 | 3 | 11 | 67 | 391 | 452 | 8.28E-16 | 73.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023545.01.T01 | A0A072TUX7 | 92.982 | 57 | 3 | 1 | 11 | 66 | 387 | 443 | 2.37e-26 | 110 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000828.01 | MsG0480023545.01 | 0.809087 | 2.250496e-50 | 1.309015e-47 |
| MsG0180002482.01 | MsG0480023545.01 | 0.807602 | 4.665443e-50 | 2.609539e-47 |
| MsG0180003952.01 | MsG0480023545.01 | 0.805172 | 1.517463e-49 | 7.973651e-47 |
| MsG0480020136.01 | MsG0480023545.01 | 0.806057 | 9.895623e-50 | 5.319294e-47 |
| MsG0480020874.01 | MsG0480023545.01 | 0.815892 | 7.325420e-52 | 5.113352e-49 |
| MsG0480022533.01 | MsG0480023545.01 | 0.812908 | 3.344923e-51 | 2.153247e-48 |
| MsG0480023545.01 | MsG0480023814.01 | 0.820685 | 6.019143e-53 | 4.795090e-50 |
| MsG0480023545.01 | MsG0880044486.01 | 0.800387 | 1.476024e-48 | 6.863988e-46 |
| MsG0480023545.01 | MsG0880047448.01 | 0.805382 | 1.371700e-49 | 7.246451e-47 |
| MsG0380016004.01 | MsG0480023545.01 | 0.801042 | 1.085184e-48 | 5.131260e-46 |
| MsG0380017680.01 | MsG0480023545.01 | 0.806749 | 7.070292e-50 | 3.868253e-47 |
| MsG0280007164.01 | MsG0480023545.01 | 0.812867 | 3.414938e-51 | 2.195798e-48 |
| MsG0280010443.01 | MsG0480023545.01 | 0.808554 | 2.925891e-50 | 1.678009e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023545.01.T01 | MTR_8g096030 | 92.982 | 57 | 3 | 1 | 11 | 66 | 387 | 443 | 6.00e-30 | 110 |
| MsG0480023545.01.T01 | MTR_5g091640 | 53.448 | 58 | 26 | 1 | 11 | 67 | 406 | 463 | 1.87e-13 | 64.3 |
| MsG0480023545.01.T01 | MTR_5g008600 | 47.368 | 57 | 29 | 1 | 11 | 66 | 439 | 495 | 1.66e-12 | 61.6 |
| MsG0480023545.01.T01 | MTR_1g039030 | 41.667 | 60 | 34 | 1 | 11 | 69 | 653 | 712 | 5.06e-12 | 60.5 |
| MsG0480023545.01.T01 | MTR_2g094430 | 45.614 | 57 | 30 | 1 | 11 | 66 | 448 | 504 | 9.19e-12 | 59.7 |
| MsG0480023545.01.T01 | MTR_2g021710 | 49.123 | 57 | 28 | 1 | 11 | 66 | 593 | 649 | 9.41e-12 | 59.7 |
| MsG0480023545.01.T01 | MTR_7g093900 | 45.312 | 64 | 34 | 1 | 5 | 67 | 514 | 577 | 2.82e-11 | 58.2 |
| MsG0480023545.01.T01 | MTR_7g093900 | 45.312 | 64 | 34 | 1 | 5 | 67 | 464 | 527 | 3.75e-11 | 57.8 |
| MsG0480023545.01.T01 | MTR_1g103840 | 46.552 | 58 | 30 | 1 | 11 | 67 | 552 | 609 | 5.63e-11 | 57.4 |
| MsG0480023545.01.T01 | MTR_2g015530 | 43.103 | 58 | 32 | 1 | 11 | 67 | 418 | 475 | 5.81e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023545.01.T01 | AT5G61800 | 59.677 | 62 | 20 | 3 | 11 | 67 | 391 | 452 | 8.44e-17 | 73.9 |
| MsG0480023545.01.T01 | AT5G42450 | 46.970 | 66 | 34 | 1 | 3 | 67 | 366 | 431 | 5.16e-12 | 60.5 |
| MsG0480023545.01.T01 | AT5G42450 | 46.970 | 66 | 34 | 1 | 3 | 67 | 282 | 347 | 6.22e-12 | 60.1 |
| MsG0480023545.01.T01 | AT3G49170 | 43.103 | 58 | 32 | 1 | 11 | 67 | 616 | 673 | 2.71e-11 | 58.2 |
| MsG0480023545.01.T01 | AT5G08510 | 47.368 | 57 | 29 | 1 | 11 | 66 | 358 | 414 | 3.52e-11 | 57.8 |
| MsG0480023545.01.T01 | AT2G22410 | 45.614 | 57 | 30 | 1 | 12 | 67 | 532 | 588 | 4.49e-11 | 57.8 |
Find 30 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGGGGTTTATTTCTTTA+TGG | 0.158926 | 4:-86711271 | MsG0480023545.01.T01:CDS |
| GAATCAAGGGAGTTGATAAA+AGG | 0.269221 | 4:-86711410 | MsG0480023545.01.T01:CDS |
| TTTGCGTGGGTTGGGTTGTT+AGG | 0.278978 | 4:-86711365 | MsG0480023545.01.T01:CDS |
| GGTGATGTGTTTGCGTGGGT+TGG | 0.343055 | 4:-86711374 | MsG0480023545.01.T01:CDS |
| AAATAAACCCCACCATCTTC+AGG | 0.349297 | 4:+86711279 | None:intergenic |
| GTGATGTGTTTGCGTGGGTT+GGG | 0.392018 | 4:-86711373 | MsG0480023545.01.T01:CDS |
| TGCTAGAGAGGGTAACATTA+TGG | 0.398113 | 4:-86711472 | MsG0480023545.01.T01:CDS |
| GAGGGTAACATTATGGTGTA+TGG | 0.456256 | 4:-86711465 | MsG0480023545.01.T01:CDS |
| GCAAACACATCACCACCATT+TGG | 0.459203 | 4:+86711383 | None:intergenic |
| CTGGGTTGATTGAAGAATCA+AGG | 0.469556 | 4:-86711424 | MsG0480023545.01.T01:CDS |
| TTATGCTAATACAGAGCAAT+GGG | 0.482819 | 4:-86711241 | MsG0480023545.01.T01:CDS |
| GCTGATATGTTGGCAAGAGC+TGG | 0.496522 | 4:-86711443 | MsG0480023545.01.T01:CDS |
| GCGTGGGTTGGGTTGTTAGG+TGG | 0.502104 | 4:-86711362 | MsG0480023545.01.T01:CDS |
| TTGTTAGGTGGATGTATACA+TGG | 0.508364 | 4:-86711350 | MsG0480023545.01.T01:CDS |
| AATCAAGGGAGTTGATAAAA+GGG | 0.510879 | 4:-86711409 | MsG0480023545.01.T01:CDS |
| AGATTAAACCTGAAGATGGT+GGG | 0.530947 | 4:-86711287 | MsG0480023545.01.T01:CDS |
| AATGAGATTAAACCTGAAGA+TGG | 0.539249 | 4:-86711291 | MsG0480023545.01.T01:CDS |
| TTGATAAAAGGGATGCCAAA+TGG | 0.547611 | 4:-86711398 | MsG0480023545.01.T01:CDS |
| TTTATGCTAATACAGAGCAA+TGG | 0.561463 | 4:-86711242 | MsG0480023545.01.T01:CDS |
| TGTGTATGGGGTTGCTAGAG+AGG | 0.563029 | 4:-86711484 | None:intergenic |
| GAGATTAAACCTGAAGATGG+TGG | 0.574744 | 4:-86711288 | MsG0480023545.01.T01:CDS |
| ATGGTGTATGGCTGATATGT+TGG | 0.579787 | 4:-86711453 | MsG0480023545.01.T01:CDS |
| ATGGTGGTGATGTGTTTGCG+TGG | 0.590125 | 4:-86711379 | MsG0480023545.01.T01:CDS |
| TGTTAGGTGGATGTATACAT+GGG | 0.593408 | 4:-86711349 | MsG0480023545.01.T01:CDS |
| TGGTGGTGATGTGTTTGCGT+GGG | 0.598296 | 4:-86711378 | MsG0480023545.01.T01:CDS |
| GATTAAACCTGAAGATGGTG+GGG | 0.603187 | 4:-86711286 | MsG0480023545.01.T01:CDS |
| TGGGTTGATTGAAGAATCAA+GGG | 0.603609 | 4:-86711423 | MsG0480023545.01.T01:CDS |
| CTGATATGTTGGCAAGAGCT+GGG | 0.606051 | 4:-86711442 | MsG0480023545.01.T01:CDS |
| GTGTATGGGGTTGCTAGAGA+GGG | 0.609242 | 4:-86711483 | None:intergenic |
| ATAAAAGGGATGCCAAATGG+TGG | 0.726229 | 4:-86711395 | MsG0480023545.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGAGATTAAACCTGAAGA+TGG | - | Chr4:86711420-86711439 | MsG0480023545.01.T01:CDS | 30.0% | |
| TTATGCTAATACAGAGCAAT+GGG | - | Chr4:86711470-86711489 | MsG0480023545.01.T01:CDS | 30.0% | |
| TTTATGCTAATACAGAGCAA+TGG | - | Chr4:86711469-86711488 | MsG0480023545.01.T01:CDS | 30.0% | |
| !! | AATCAAGGGAGTTGATAAAA+GGG | - | Chr4:86711302-86711321 | MsG0480023545.01.T01:CDS | 30.0% |
| AGATTAAACCTGAAGATGGT+GGG | - | Chr4:86711424-86711443 | MsG0480023545.01.T01:CDS | 35.0% | |
| TGTTAGGTGGATGTATACAT+GGG | - | Chr4:86711362-86711381 | MsG0480023545.01.T01:CDS | 35.0% | |
| TTGATAAAAGGGATGCCAAA+TGG | - | Chr4:86711313-86711332 | MsG0480023545.01.T01:CDS | 35.0% | |
| TTGTTAGGTGGATGTATACA+TGG | - | Chr4:86711361-86711380 | MsG0480023545.01.T01:CDS | 35.0% | |
| ! | TGGTGGGGTTTATTTCTTTA+TGG | - | Chr4:86711440-86711459 | MsG0480023545.01.T01:CDS | 35.0% |
| !! | GAATCAAGGGAGTTGATAAA+AGG | - | Chr4:86711301-86711320 | MsG0480023545.01.T01:CDS | 35.0% |
| !! | TGGGTTGATTGAAGAATCAA+GGG | - | Chr4:86711288-86711307 | MsG0480023545.01.T01:CDS | 35.0% |
| AAATAAACCCCACCATCTTC+AGG | + | Chr4:86711435-86711454 | None:intergenic | 40.0% | |
| ATAAAAGGGATGCCAAATGG+TGG | - | Chr4:86711316-86711335 | MsG0480023545.01.T01:CDS | 40.0% | |
| ATGGTGTATGGCTGATATGT+TGG | - | Chr4:86711258-86711277 | MsG0480023545.01.T01:CDS | 40.0% | |
| GAGATTAAACCTGAAGATGG+TGG | - | Chr4:86711423-86711442 | MsG0480023545.01.T01:CDS | 40.0% | |
| GAGGGTAACATTATGGTGTA+TGG | - | Chr4:86711246-86711265 | MsG0480023545.01.T01:CDS | 40.0% | |
| GATTAAACCTGAAGATGGTG+GGG | - | Chr4:86711425-86711444 | MsG0480023545.01.T01:CDS | 40.0% | |
| TGCTAGAGAGGGTAACATTA+TGG | - | Chr4:86711239-86711258 | MsG0480023545.01.T01:CDS | 40.0% | |
| !! | CTGGGTTGATTGAAGAATCA+AGG | - | Chr4:86711287-86711306 | MsG0480023545.01.T01:CDS | 40.0% |
| CTGATATGTTGGCAAGAGCT+GGG | - | Chr4:86711269-86711288 | MsG0480023545.01.T01:CDS | 45.0% | |
| GCAAACACATCACCACCATT+TGG | + | Chr4:86711331-86711350 | None:intergenic | 45.0% | |
| GCTGATATGTTGGCAAGAGC+TGG | - | Chr4:86711268-86711287 | MsG0480023545.01.T01:CDS | 50.0% | |
| GTGATGTGTTTGCGTGGGTT+GGG | - | Chr4:86711338-86711357 | MsG0480023545.01.T01:CDS | 50.0% | |
| ! | ATGGTGGTGATGTGTTTGCG+TGG | - | Chr4:86711332-86711351 | MsG0480023545.01.T01:CDS | 50.0% |
| ! | TGGTGGTGATGTGTTTGCGT+GGG | - | Chr4:86711333-86711352 | MsG0480023545.01.T01:CDS | 50.0% |
| !! | TTTGCGTGGGTTGGGTTGTT+AGG | - | Chr4:86711346-86711365 | MsG0480023545.01.T01:CDS | 50.0% |
| GGTGATGTGTTTGCGTGGGT+TGG | - | Chr4:86711337-86711356 | MsG0480023545.01.T01:CDS | 55.0% | |
| !! | GCGTGGGTTGGGTTGTTAGG+TGG | - | Chr4:86711349-86711368 | MsG0480023545.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 86711232 | 86711501 | 86711232 | ID=MsG0480023545.01;Name=MsG0480023545.01 |
| Chr4 | mRNA | 86711232 | 86711501 | 86711232 | ID=MsG0480023545.01.T01;Parent=MsG0480023545.01;Name=MsG0480023545.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|89 |
| Chr4 | exon | 86711232 | 86711501 | 86711232 | ID=MsG0480023545.01.T01:exon:6480;Parent=MsG0480023545.01.T01 |
| Chr4 | CDS | 86711232 | 86711501 | 86711232 | ID=MsG0480023545.01.T01:cds;Parent=MsG0480023545.01.T01 |
| Gene Sequence |
| Protein sequence |
