AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020136.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020136.01.T01 MTR_4g046047 80.978 184 5 1 1 184 1 154 6.03e-103 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020136.01.T01 AT1G03650 57.143 182 45 2 6 184 6 157 2.59e-66 200

Find 43 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATGCAGGGTTCGAGATTC+TGG 0.158668 4:+34844508 MsG0480020136.01.T01:CDS
CAGGTTATGTCATGTATTCT+TGG 0.193464 4:+34844211 MsG0480020136.01.T01:CDS
AGTGGAAGAGATAGTGAAAT+TGG 0.247431 4:+34844083 MsG0480020136.01.T01:CDS
CACGTTGATGCCCAACTTGC+AGG 0.261704 4:+34844192 MsG0480020136.01.T01:CDS
TGGCTCCGCCACTGGCTAAA+TGG 0.341028 4:-34844538 None:intergenic
CATGTTGACTGTTTGGTTGA+AGG 0.355783 4:+34845610 MsG0480020136.01.T01:CDS
GAAGCGAGTGATTCGTGTTT+GGG 0.367842 4:-34844120 None:intergenic
GGAAGCGAGTGATTCGTGTT+TGG 0.373847 4:-34844121 None:intergenic
TAGCTTTCATGTTGACTGTT+TGG 0.388139 4:+34845603 MsG0480020136.01.T01:CDS
CAGTTGGTAAGCTTGGATAT+TGG 0.392525 4:+34844486 MsG0480020136.01.T01:intron
TATGCAGGGTTCGAGATTCT+GGG 0.431050 4:+34844509 MsG0480020136.01.T01:CDS
CATGACATAACCTGCAAGTT+GGG 0.434152 4:-34844202 None:intergenic
AAGCTTGGATATTGGTATGC+AGG 0.445687 4:+34844494 MsG0480020136.01.T01:CDS
TGTGACTCAGTTGGTAAGCT+TGG 0.456101 4:+34844479 MsG0480020136.01.T01:intron
AATAATTCTGGCTCCGCCAC+TGG 0.462444 4:-34844546 None:intergenic
TGGACTTCGATTCAAATTGA+AGG 0.474763 4:+34845671 MsG0480020136.01.T01:CDS
AGGGCATGGAGAGGTTCTAC+TGG 0.510423 4:+34845483 MsG0480020136.01.T01:CDS
AAATGCTTATAGAATGTACT+TGG 0.517363 4:+34845651 MsG0480020136.01.T01:CDS
CAATGGAGGAGGCAAGGGCA+TGG 0.517572 4:+34845469 MsG0480020136.01.T01:CDS
TCTTGTACAGATTCAGCGCA+GGG 0.523854 4:-34845576 None:intergenic
AAGGAGCAATGGAGGAGGCA+AGG 0.528602 4:+34845463 MsG0480020136.01.T01:CDS
ACATGACATAACCTGCAAGT+TGG 0.533996 4:-34844203 None:intergenic
GGAGTTGGAACCTGCGAGCT+TGG 0.538376 4:-34844259 None:intergenic
GCAGAACAAGAAAAGTTTCG+CGG 0.541873 4:+34845524 MsG0480020136.01.T01:CDS
AGCTTGGATATTGGTATGCA+GGG 0.542315 4:+34844495 MsG0480020136.01.T01:CDS
TGGAGAGGTTCTACTGGAAG+CGG 0.545320 4:+34845489 MsG0480020136.01.T01:CDS
GCTTCTATCACCAAGCTCGC+AGG 0.549548 4:+34844249 MsG0480020136.01.T01:CDS
TTCTTGTACAGATTCAGCGC+AGG 0.552919 4:-34845577 None:intergenic
GCCAGAATTATTATAAAACC+TGG 0.561206 4:+34844557 MsG0480020136.01.T01:CDS
TGCTTCACGTCGATCCGTTG+AGG 0.562820 4:+34845551 MsG0480020136.01.T01:CDS
ATAGAAGCATACAATGAAGA+AGG 0.562971 4:-34844234 None:intergenic
AGGAGCAATGGAGGAGGCAA+GGG 0.571699 4:+34845464 MsG0480020136.01.T01:CDS
CAGAAACAGTGGTAAAGTAG+TGG 0.572015 4:+34844065 MsG0480020136.01.T01:CDS
CGCATGAGTGATGGCAAATG+TGG 0.573974 4:+34844026 None:intergenic
CTTCTGTAGTGAAGGAGCAA+TGG 0.596774 4:+34845452 MsG0480020136.01.T01:intron
CTGTAGTGAAGGAGCAATGG+AGG 0.602271 4:+34845455 MsG0480020136.01.T01:intron
TAGTGAAGGAGCAATGGAGG+AGG 0.603189 4:+34845458 MsG0480020136.01.T01:intron
GGTTCGAACCATTTAGCCAG+TGG 0.611235 4:+34844530 MsG0480020136.01.T01:CDS
TTCAGCGCAGGGGTCCTCAA+CGG 0.614591 4:-34845565 None:intergenic
TCGAACCATTTAGCCAGTGG+CGG 0.621225 4:+34844533 MsG0480020136.01.T01:CDS
GAGGAGGCAAGGGCATGGAG+AGG 0.640974 4:+34845474 MsG0480020136.01.T01:CDS
AATGAACTTGACAGAAACAG+TGG 0.667655 4:+34844054 MsG0480020136.01.T01:CDS
CTTGTACAGATTCAGCGCAG+GGG 0.671497 4:-34845575 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAATTATTCAATTTAT+AGG + Chr4:34844649-34844668 MsG0480020136.01.T01:intron 10.0%
!! AAGAAATTATTCAATTTATA+GGG + Chr4:34844650-34844669 MsG0480020136.01.T01:intron 10.0%
!! AATTAACTATCTTTAATATA+AGG + Chr4:34845313-34845332 MsG0480020136.01.T01:intron 10.0%
!! ATTAACTATCTTTAATATAA+GGG + Chr4:34845314-34845333 MsG0480020136.01.T01:intron 10.0%
!! AGAAATTATTCAATTTATAG+GGG + Chr4:34844651-34844670 MsG0480020136.01.T01:intron 15.0%
!!! AATAAATAAGGGTATAATAA+TGG - Chr4:34844998-34845017 None:intergenic 15.0%
!! AAAAATAATAAATGCTGCAT+TGG - Chr4:34844973-34844992 None:intergenic 20.0%
!! CTACTAACTACAATTAATAA+GGG + Chr4:34845041-34845060 MsG0480020136.01.T01:intron 20.0%
!! GGATGAAATTCAATAAATAA+GGG - Chr4:34845009-34845028 None:intergenic 20.0%
!! TCTACTAACTACAATTAATA+AGG + Chr4:34845040-34845059 MsG0480020136.01.T01:intron 20.0%
!! TGGATGAAATTCAATAAATA+AGG - Chr4:34845010-34845029 None:intergenic 20.0%
!!! ATTAATTGTGTTGATTTTGA+TGG - Chr4:34845093-34845112 None:intergenic 20.0%
!!! ATTTTGAGAAAAAGAAAAAG+TGG - Chr4:34844932-34844951 None:intergenic 20.0%
! AAACGACTATTATTTAGAGA+CGG + Chr4:34845142-34845161 MsG0480020136.01.T01:intron 25.0%
! AAATGCTTATAGAATGTACT+TGG + Chr4:34845651-34845670 MsG0480020136.01.T01:CDS 25.0%
! AAGAGACAATTATTATGAGA+CGG - Chr4:34844907-34844926 None:intergenic 25.0%
! ACAATATGGGTATGTAATAT+GGG + Chr4:34845418-34845437 MsG0480020136.01.T01:intron 25.0%
! ATACAAGCATTTCATTAGTT+GGG - Chr4:34845371-34845390 None:intergenic 25.0%
! CTAAACTATTGAAAGATAGT+AGG - Chr4:34844834-34844853 None:intergenic 25.0%
! CTCTAAATAATAGTCGTTTA+AGG - Chr4:34845141-34845160 None:intergenic 25.0%
!! AAAATTGTCTAAGTGTGTTT+TGG + Chr4:34844324-34844343 MsG0480020136.01.T01:intron 25.0%
!!! ACCAGGTTTTATAATAATTC+TGG - Chr4:34844561-34844580 None:intergenic 25.0%
!!! CAATTTTTCAAACAGCTTAT+AGG - Chr4:34844311-34844330 None:intergenic 25.0%
!!! TTTGAGTTCATTTTCAAAGA+AGG - Chr4:34844145-34844164 None:intergenic 25.0%
AAATTGCAAAAACATTCAGC+CGG + Chr4:34844707-34844726 MsG0480020136.01.T01:intron 30.0%
AACATTTGCAACCCATTTAT+AGG + Chr4:34844586-34844605 MsG0480020136.01.T01:intron 30.0%
ACATTTGCAACCCATTTATA+GGG + Chr4:34844587-34844606 MsG0480020136.01.T01:intron 30.0%
AGGTTTATGCACGATTATTA+AGG - Chr4:34845121-34845140 None:intergenic 30.0%
AGTTAGTAGAGTAGTTAAGT+TGG - Chr4:34845030-34845049 None:intergenic 30.0%
CACAATATGGGTATGTAATA+TGG + Chr4:34845417-34845436 MsG0480020136.01.T01:intron 30.0%
CATACAAGCATTTCATTAGT+TGG - Chr4:34845372-34845391 None:intergenic 30.0%
GCCAGAATTATTATAAAACC+TGG + Chr4:34844557-34844576 MsG0480020136.01.T01:CDS 30.0%
TAATACATCAACTTAGTCGT+TGG + Chr4:34844797-34844816 MsG0480020136.01.T01:intron 30.0%
TTATGCAAACCCCTATAAAT+GGG - Chr4:34844600-34844619 None:intergenic 30.0%
! ATAGAAGCATACAATGAAGA+AGG - Chr4:34844237-34844256 None:intergenic 30.0%
! GTATGTAATATGGGATGTTT+TGG + Chr4:34845427-34845446 MsG0480020136.01.T01:intron 30.0%
! TTGTTGGTTACTCACAATAT+GGG + Chr4:34845405-34845424 MsG0480020136.01.T01:intron 30.0%
! TTTGTTGGTTACTCACAATA+TGG + Chr4:34845404-34845423 MsG0480020136.01.T01:intron 30.0%
!! ACGACTAAGTTGATGTATTA+AGG - Chr4:34844797-34844816 None:intergenic 30.0%
!! ACTAAGTTGATGTATTAAGG+GGG - Chr4:34844794-34844813 None:intergenic 30.0%
!! CGACTAAGTTGATGTATTAA+GGG - Chr4:34844796-34844815 None:intergenic 30.0%
!! GACTAAGTTGATGTATTAAG+GGG - Chr4:34844795-34844814 None:intergenic 30.0%
!!! GTTGCAAATGTTTTTTTACC+AGG - Chr4:34844578-34844597 None:intergenic 30.0%
AATGAACTTGACAGAAACAG+TGG + Chr4:34844054-34844073 MsG0480020136.01.T01:CDS 35.0%
AGACAATTATTATGAGACGG+AGG - Chr4:34844904-34844923 None:intergenic 35.0%
AGCATAAAAAAGAGTGGAGT+TGG - Chr4:34844277-34844296 None:intergenic 35.0%
AGTGGAAGAGATAGTGAAAT+TGG + Chr4:34844083-34844102 MsG0480020136.01.T01:CDS 35.0%
CAGGTTATGTCATGTATTCT+TGG + Chr4:34844211-34844230 MsG0480020136.01.T01:CDS 35.0%
CATTGTCGATATAGTTAGGT+TGG - Chr4:34845280-34845299 None:intergenic 35.0%
CATTTGCAACCCATTTATAG+GGG + Chr4:34844588-34844607 MsG0480020136.01.T01:intron 35.0%
CTATATCGACAATGTCAGTT+AGG + Chr4:34845286-34845305 MsG0480020136.01.T01:intron 35.0%
CTGACATTGTCGATATAGTT+AGG - Chr4:34845284-34845303 None:intergenic 35.0%
CTTATGCAAACCCCTATAAA+TGG - Chr4:34844601-34844620 None:intergenic 35.0%
GAACTCAAAAAGAAGAACAG+TGG + Chr4:34844156-34844175 MsG0480020136.01.T01:CDS 35.0%
GACAATTATTATGAGACGGA+GGG - Chr4:34844903-34844922 None:intergenic 35.0%
GACTATTATTTAGAGACGGA+GGG + Chr4:34845146-34845165 MsG0480020136.01.T01:intron 35.0%
GTGAGTAACCAACAAATCAA+AGG - Chr4:34845400-34845419 None:intergenic 35.0%
GTTGACAGCATAAAAAAGAG+TGG - Chr4:34844283-34844302 None:intergenic 35.0%
TGATTTGAGCAAAAGGCTAA+AGG - Chr4:34844761-34844780 None:intergenic 35.0%
TTGTGTGTGTGAATTTCATG+TGG - Chr4:34845223-34845242 None:intergenic 35.0%
! TAGCTTTCATGTTGACTGTT+TGG + Chr4:34845603-34845622 MsG0480020136.01.T01:CDS 35.0%
! TTTTGTAAGTGTGACTCAGT+TGG + Chr4:34844470-34844489 MsG0480020136.01.T01:intron 35.0%
!! ACTCAACTGATTTGAGCAAA+AGG - Chr4:34844768-34844787 None:intergenic 35.0%
!! ATGCGAATCCTTTGATTTGT+TGG + Chr4:34845389-34845408 MsG0480020136.01.T01:intron 35.0%
!!! TTTTGGTTCTTCTGTAGTGA+AGG + Chr4:34845444-34845463 MsG0480020136.01.T01:intron 35.0%
AAGCTTGGATATTGGTATGC+AGG + Chr4:34844494-34844513 MsG0480020136.01.T01:CDS 40.0%
AATTCACACACACAACTGCA+AGG + Chr4:34845228-34845247 MsG0480020136.01.T01:intron 40.0%
ACATGACATAACCTGCAAGT+TGG - Chr4:34844206-34844225 None:intergenic 40.0%
AGCTTGGATATTGGTATGCA+GGG + Chr4:34844495-34844514 MsG0480020136.01.T01:CDS 40.0%
CAGAAACAGTGGTAAAGTAG+TGG + Chr4:34844065-34844084 MsG0480020136.01.T01:CDS 40.0%
CATGACATAACCTGCAAGTT+GGG - Chr4:34844205-34844224 None:intergenic 40.0%
CGACTATTATTTAGAGACGG+AGG + Chr4:34845145-34845164 MsG0480020136.01.T01:intron 40.0%
GCAGAACAAGAAAAGTTTCG+CGG + Chr4:34845524-34845543 MsG0480020136.01.T01:CDS 40.0%
TGAGCAAAAGGCTAAAGGTA+GGG - Chr4:34844756-34844775 None:intergenic 40.0%
TTGAGCAAAAGGCTAAAGGT+AGG - Chr4:34844757-34844776 None:intergenic 40.0%
! CATGTTGACTGTTTGGTTGA+AGG + Chr4:34845610-34845629 MsG0480020136.01.T01:CDS 40.0%
!! CAGTTGGTAAGCTTGGATAT+TGG + Chr4:34844486-34844505 MsG0480020136.01.T01:intron 40.0%
ACACACAACTGCAAGGTCTT+GGG + Chr4:34845235-34845254 MsG0480020136.01.T01:intron 45.0%
CTTCTGTAGTGAAGGAGCAA+TGG + Chr4:34845452-34845471 MsG0480020136.01.T01:intron 45.0%
GAAGCGAGTGATTCGTGTTT+GGG - Chr4:34844123-34844142 None:intergenic 45.0%
GAGCAAAAGGCTAAAGGTAG+GGG - Chr4:34844755-34844774 None:intergenic 45.0%
TATGCAGGGTTCGAGATTCT+GGG + Chr4:34844509-34844528 MsG0480020136.01.T01:CDS 45.0%
TCTTGTACAGATTCAGCGCA+GGG - Chr4:34845579-34845598 None:intergenic 45.0%
TTCTTGTACAGATTCAGCGC+AGG - Chr4:34845580-34845599 None:intergenic 45.0%
!! TGTGACTCAGTTGGTAAGCT+TGG + Chr4:34844479-34844498 MsG0480020136.01.T01:intron 45.0%
CAAAAGGCTAAAGGTAGGGG+CGG - Chr4:34844752-34844771 None:intergenic 50.0%
CACACACAACTGCAAGGTCT+TGG + Chr4:34845234-34845253 MsG0480020136.01.T01:intron 50.0%
CTGTAGTGAAGGAGCAATGG+AGG + Chr4:34845455-34845474 MsG0480020136.01.T01:intron 50.0%
CTTGTACAGATTCAGCGCAG+GGG - Chr4:34845578-34845597 None:intergenic 50.0%
GGAAGCGAGTGATTCGTGTT+TGG - Chr4:34844124-34844143 None:intergenic 50.0%
GGTTCGAACCATTTAGCCAG+TGG + Chr4:34844530-34844549 MsG0480020136.01.T01:CDS 50.0%
GTATGCAGGGTTCGAGATTC+TGG + Chr4:34844508-34844527 MsG0480020136.01.T01:CDS 50.0%
TAAAGGTAGGGGCGGTTCTA+AGG - Chr4:34844744-34844763 None:intergenic 50.0%
TAGTGAAGGAGCAATGGAGG+AGG + Chr4:34845458-34845477 MsG0480020136.01.T01:intron 50.0%
TCGAACCATTTAGCCAGTGG+CGG + Chr4:34844533-34844552 MsG0480020136.01.T01:CDS 50.0%
TGGAGAGGTTCTACTGGAAG+CGG + Chr4:34845489-34845508 MsG0480020136.01.T01:CDS 50.0%
!! AATAATTCTGGCTCCGCCAC+TGG - Chr4:34844549-34844568 None:intergenic 50.0%
AAAACATTCAGCCGGAGCCC+AGG + Chr4:34844715-34844734 MsG0480020136.01.T01:intron 55.0%
AAACATTCAGCCGGAGCCCA+GGG + Chr4:34844716-34844735 MsG0480020136.01.T01:intron 55.0%
AAGGAGCAATGGAGGAGGCA+AGG + Chr4:34845463-34845482 MsG0480020136.01.T01:CDS 55.0%
AGGAGCAATGGAGGAGGCAA+GGG + Chr4:34845464-34845483 MsG0480020136.01.T01:CDS 55.0%
AGGGCATGGAGAGGTTCTAC+TGG + Chr4:34845483-34845502 MsG0480020136.01.T01:CDS 55.0%
CACGTTGATGCCCAACTTGC+AGG + Chr4:34844192-34844211 MsG0480020136.01.T01:CDS 55.0%
GCTTCTATCACCAAGCTCGC+AGG + Chr4:34844249-34844268 MsG0480020136.01.T01:CDS 55.0%
TGCTTCACGTCGATCCGTTG+AGG + Chr4:34845551-34845570 MsG0480020136.01.T01:CDS 55.0%
AACATTCAGCCGGAGCCCAG+GGG + Chr4:34844717-34844736 MsG0480020136.01.T01:intron 60.0%
CAATGGAGGAGGCAAGGGCA+TGG + Chr4:34845469-34845488 MsG0480020136.01.T01:CDS 60.0%
GGAGTTGGAACCTGCGAGCT+TGG - Chr4:34844262-34844281 None:intergenic 60.0%
TTCAGCGCAGGGGTCCTCAA+CGG - Chr4:34845568-34845587 None:intergenic 60.0%
! TGGCTCCGCCACTGGCTAAA+TGG - Chr4:34844541-34844560 None:intergenic 60.0%
ACATTCAGCCGGAGCCCAGG+GGG + Chr4:34844718-34844737 MsG0480020136.01.T01:intron 65.0%
! GAGGAGGCAAGGGCATGGAG+AGG + Chr4:34845474-34845493 MsG0480020136.01.T01:CDS 65.0%
!! TTCTAAGGCCCCCTGGGCTC+CGG - Chr4:34844729-34844748 None:intergenic 65.0%
!! GGGCGGTTCTAAGGCCCCCT+GGG - Chr4:34844735-34844754 None:intergenic 70.0%
!! GGGGCGGTTCTAAGGCCCCC+TGG - Chr4:34844736-34844755 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 34844036 34845690 34844036 ID=MsG0480020136.01;Name=MsG0480020136.01
Chr4 mRNA 34844036 34845690 34844036 ID=MsG0480020136.01.T01;Parent=MsG0480020136.01;Name=MsG0480020136.01.T01;_AED=0.46;_eAED=0.56;_QI=0|0|0|1|1|1|3|0|184
Chr4 exon 34844036 34844270 34844036 ID=MsG0480020136.01.T01:exon:15446;Parent=MsG0480020136.01.T01
Chr4 exon 34844489 34844578 34844489 ID=MsG0480020136.01.T01:exon:15445;Parent=MsG0480020136.01.T01
Chr4 exon 34845461 34845690 34845461 ID=MsG0480020136.01.T01:exon:15444;Parent=MsG0480020136.01.T01
Chr4 CDS 34844036 34844270 34844036 ID=MsG0480020136.01.T01:cds;Parent=MsG0480020136.01.T01
Chr4 CDS 34844489 34844578 34844489 ID=MsG0480020136.01.T01:cds;Parent=MsG0480020136.01.T01
Chr4 CDS 34845461 34845690 34845461 ID=MsG0480020136.01.T01:cds;Parent=MsG0480020136.01.T01
Gene Sequence

>MsG0480020136.01.T01

ATGGCAAATGTGGAGATTAATGAACTTGACAGAAACAGTGGTAAAGTAGTGGAAGAGATAGTGAAATTGGAGAGAAAGATATTTCCCAAACACGAATCACTCGCTTCCTTCTTTGAAAATGAACTCAAAAAGAAGAACAGTGGATTGCTTTATCTTCACGTTGATGCCCAACTTGCAGGTTATGTCATGTATTCTTGGCCTTCTTCATTGTATGCTTCTATCACCAAGCTCGCAGTTGGTAAGCTTGGATATTGGTATGCAGGGTTCGAGATTCTGGGTTCGAACCATTTAGCCAGTGGCGGAGCCAGAATTATTATAAAACCTGTGAAGGAGCAATGGAGGAGGCAAGGGCATGGAGAGGTTCTACTGGAAGCGGCAATCGAGAAATGCAGAACAAGAAAAGTTTCGCGGATAATGCTTCACGTCGATCCGTTGAGGACCCCTGCGCTGAATCTGTACAAGAAGCATAGCTTTCATGTTGACTGTTTGGTTGAAGGCTATTACTCTTCTGATAGAAATGCTTATAGAATGTACTTGGACTTCGATTCAAATTGA

Protein sequence

>MsG0480020136.01.T01

MANVEINELDRNSGKVVEEIVKLERKIFPKHESLASFFENELKKKNSGLLYLHVDAQLAGYVMYSWPSSLYASITKLAVGKLGYWYAGFEILGSNHLASGGARIIIKPVKEQWRRQGHGEVLLEAAIEKCRTRKVSRIMLHVDPLRTPALNLYKKHSFHVDCLVEGYYSSDRNAYRMYLDFDSN*