AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024474.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024474.01.T01 MTR_5g011800 96.087 230 8 1 4 233 1 229 1.40e-163 451
MsG0580024474.01.T01 MTR_5g011820 90.129 233 22 1 1 233 1 232 2.24e-154 428
MsG0580024474.01.T01 MTR_4g102220 58.898 236 80 6 4 233 1 225 9.91e-80 239
MsG0580024474.01.T01 MTR_4g024370 51.055 237 104 5 4 233 1 232 5.18e-74 224
MsG0580024474.01.T01 MTR_4g024370 51.055 237 104 5 4 233 1 232 5.18e-74 224
MsG0580024474.01.T01 MTR_6g060390 29.130 230 137 9 8 232 4 212 2.43e-22 91.7
MsG0580024474.01.T01 MTR_6g060440 29.565 230 134 10 8 232 4 210 1.51e-21 89.4
MsG0580024474.01.T01 MTR_7g117380 28.384 229 150 7 8 232 3 221 3.87e-19 83.2
MsG0580024474.01.T01 MTR_1g076800 25.764 229 147 8 7 232 3 211 6.86e-19 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024474.01.T01 AT2G45360 52.381 231 93 5 4 233 1 215 1.13e-72 220
MsG0580024474.01.T01 AT3G60780 50.215 233 98 6 4 233 1 218 2.07e-69 212
MsG0580024474.01.T01 AT1G62840 47.303 241 99 8 4 233 1 224 1.17e-54 175
MsG0580024474.01.T01 AT1G12320 43.290 231 103 7 4 229 1 208 2.41e-45 150

Find 66 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGGGATGTTCACTTTATT+AGG 0.233385 5:-5409758 MsG0580024474.01.T01:CDS
TTTATTTCTAAGATGGATTC+TGG 0.260989 5:-5409969 MsG0580024474.01.T01:CDS
TATGAGTTAGTTGCTCTCTC+TGG 0.291619 5:+5410404 None:intergenic
TTTGATTGGAATAGATTTCT+TGG 0.340143 5:-5410084 MsG0580024474.01.T01:CDS
ATGGCGGAGCGTGGTGGCGC+GGG 0.351116 5:-5409940 MsG0580024474.01.T01:CDS
AGATGGATTCTGGTTATATA+TGG 0.373750 5:-5409959 MsG0580024474.01.T01:CDS
TGGTATGTCACCGGAAGTTT+TGG 0.390155 5:-5410135 MsG0580024474.01.T01:CDS
ATTTCTTGGTTGTTGATTCT+CGG 0.396616 5:-5410070 MsG0580024474.01.T01:CDS
TGAAAGACATGAAACTTGTT+TGG 0.407611 5:-5410430 MsG0580024474.01.T01:CDS
AGCACGTCCCTTATGCTTCA+TGG 0.413780 5:+5409818 None:intergenic
TGTTCGGTCAGTATTTCTTC+CGG 0.416161 5:-5409904 MsG0580024474.01.T01:CDS
GAAGTGATGGATGGTTTGAT+TGG 0.429509 5:-5410098 MsG0580024474.01.T01:CDS
ATGAGTTAGTTGCTCTCTCT+GGG 0.435811 5:+5410405 None:intergenic
AAGCATGTTGATCAACGATC+AGG 0.439670 5:-5409777 MsG0580024474.01.T01:CDS
TCCGATCTTGACCGTTCATC+AGG 0.440907 5:+5410182 None:intergenic
GGTTCTCCCACCAAAACTTC+CGG 0.450365 5:+5410125 None:intergenic
AGAATCCGGTGCGGCGGAGC+TGG 0.456364 5:-5410336 MsG0580024474.01.T01:CDS
TGGATAAACCAACACTCGTT+GGG 0.457594 5:+5410246 None:intergenic
GGGGATCACGACGACTTGTT+CGG 0.470863 5:-5409920 MsG0580024474.01.T01:CDS
ACCTGTGTGACCGCTTGCTA+TGG 0.473046 5:+5410226 None:intergenic
TTCTCTAGGAAGGTGATTGA+TGG 0.483897 5:+5410355 None:intergenic
GGCGGAGCTGGTATCATCTA+TGG 0.484116 5:-5410324 MsG0580024474.01.T01:CDS
AATCACCTTCCTAGAGAATC+CGG 0.490375 5:-5410350 MsG0580024474.01.T01:CDS
AAGGTGATTGATGGTAGTTA+TGG 0.490442 5:+5410364 None:intergenic
GACGTGCTGTTCATAATAGA+TGG 0.491292 5:-5409803 MsG0580024474.01.T01:CDS
TGTTACGGCCATGAAGCATA+AGG 0.493546 5:-5409826 MsG0580024474.01.T01:CDS
TGGCGGAGCGTGGTGGCGCG+GGG 0.493800 5:-5409939 MsG0580024474.01.T01:CDS
AGTATTTCTTCCGGTGGGAA+AGG 0.497245 5:-5409895 MsG0580024474.01.T01:CDS
TGGTGGTGACAACTCTGTTA+CGG 0.498458 5:-5409841 MsG0580024474.01.T01:CDS
TTAGCAAAGTTGAGTGGTAA+AGG 0.503228 5:-5410020 MsG0580024474.01.T01:CDS
GTATTTCTTCCGGTGGGAAA+GGG 0.503243 5:-5409894 MsG0580024474.01.T01:CDS
TATGGCGGAGCGTGGTGGCG+CGG 0.506489 5:-5409941 MsG0580024474.01.T01:CDS
AAACCATCCATCACTTCCTC+CGG 0.520767 5:+5410104 None:intergenic
CTGGTATCATCTATGGCAGC+AGG 0.522629 5:-5410317 MsG0580024474.01.T01:CDS
AACAGAGTTGTCACCACCAC+CGG 0.522950 5:+5409845 None:intergenic
GGTGGGAAAGGGAATTCATA+TGG 0.529266 5:-5409883 MsG0580024474.01.T01:CDS
GTTACGGCCATGAAGCATAA+GGG 0.544486 5:-5409825 MsG0580024474.01.T01:CDS
TCCTGATGAACGGTCAAGAT+CGG 0.549497 5:-5410183 MsG0580024474.01.T01:CDS
GGGAGAACCGGAGGAAGTGA+TGG 0.550221 5:-5410111 MsG0580024474.01.T01:CDS
TGATACCAGCTCCGCCGCAC+CGG 0.550329 5:+5410331 None:intergenic
ATGGATAAACCAACACTCGT+TGG 0.556717 5:+5410245 None:intergenic
TCGGTCAGTATTTCTTCCGG+TGG 0.561365 5:-5409901 MsG0580024474.01.T01:CDS
AGCATGTTGATCAACGATCA+GGG 0.563826 5:-5409776 MsG0580024474.01.T01:CDS
TCCTAGAGAATCCGGTGCGG+CGG 0.565345 5:-5410342 MsG0580024474.01.T01:CDS
GGAGTTATCCCAACGAGTGT+TGG 0.567790 5:-5410254 MsG0580024474.01.T01:CDS
CGGTCAGTATTTCTTCCGGT+GGG 0.578946 5:-5409900 MsG0580024474.01.T01:CDS
TCCGCCGCACCGGATTCTCT+AGG 0.590144 5:+5410341 None:intergenic
CTCAGCTGATAGTAGAAACA+TGG 0.594393 5:-5410286 MsG0580024474.01.T01:CDS
GGGAGAAGCTGGTATGTCAC+CGG 0.594972 5:-5410144 MsG0580024474.01.T01:CDS
ATATGAATTCCCTTTCCCAC+CGG 0.597083 5:+5409885 None:intergenic
TTGAGGTTAGCAAAGTTGAG+TGG 0.609267 5:-5410026 MsG0580024474.01.T01:CDS
TTATATATGGCGGAGCGTGG+TGG 0.612287 5:-5409946 MsG0580024474.01.T01:CDS
CCGCACCGGATTCTCTAGGA+AGG 0.614758 5:+5410345 None:intergenic
GAACCGGAGGAAGTGATGGA+TGG 0.618455 5:-5410107 MsG0580024474.01.T01:CDS
TCCATAGCAAGCGGTCACAC+AGG 0.622700 5:-5410227 MsG0580024474.01.T01:CDS
TGGTTATATATGGCGGAGCG+TGG 0.623341 5:-5409949 MsG0580024474.01.T01:CDS
TATCATCTATGGCAGCAGGA+TGG 0.632923 5:-5410313 MsG0580024474.01.T01:CDS
TGTGCATAGTTCCTGATGAA+CGG 0.633781 5:-5410193 MsG0580024474.01.T01:CDS
ATAGCAAGCGGTCACACAGG+TGG 0.636263 5:-5410224 MsG0580024474.01.T01:CDS
TGGATTCTGGTTATATATGG+CGG 0.643639 5:-5409956 MsG0580024474.01.T01:CDS
GCATGTTGATCAACGATCAG+GGG 0.659538 5:-5409775 MsG0580024474.01.T01:CDS
GTTGGTTTATCCATAGCAAG+CGG 0.671936 5:-5410236 MsG0580024474.01.T01:CDS
TGAGTTAGTTGCTCTCTCTG+GGG 0.683268 5:+5410406 None:intergenic
ATAGTAGAAACATGGTCACA+CGG 0.693003 5:-5410278 MsG0580024474.01.T01:CDS
CCTTCCTAGAGAATCCGGTG+CGG 0.793946 5:-5410345 MsG0580024474.01.T01:CDS
GTAGAAACATGGTCACACGG+CGG 0.823354 5:-5410275 MsG0580024474.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGCTAATTTTATTTCTAAGA+TGG - Chr5:5410207-5410226 MsG0580024474.01.T01:CDS 20.0%
! TTTGATTGGAATAGATTTCT+TGG - Chr5:5410099-5410118 MsG0580024474.01.T01:CDS 25.0%
!!! TTTATTTCTAAGATGGATTC+TGG - Chr5:5410214-5410233 MsG0580024474.01.T01:CDS 25.0%
! AATTCATATGGCACACTTTT+CGG - Chr5:5410312-5410331 MsG0580024474.01.T01:CDS 30.0%
! AGATGGATTCTGGTTATATA+TGG - Chr5:5410224-5410243 MsG0580024474.01.T01:CDS 30.0%
! TGAAAGACATGAAACTTGTT+TGG - Chr5:5409753-5409772 MsG0580024474.01.T01:CDS 30.0%
!! ATTTCTTGGTTGTTGATTCT+CGG - Chr5:5410113-5410132 MsG0580024474.01.T01:CDS 30.0%
!!! AAGATTTTACAAGAGTGTTG+AGG - Chr5:5410140-5410159 MsG0580024474.01.T01:CDS 30.0%
ATAGTAGAAACATGGTCACA+CGG - Chr5:5409905-5409924 MsG0580024474.01.T01:CDS 35.0%
ATCGGAGTATGCAAAAAACA+TGG - Chr5:5410018-5410037 MsG0580024474.01.T01:CDS 35.0%
TCGGAGTATGCAAAAAACAT+GGG - Chr5:5410019-5410038 MsG0580024474.01.T01:CDS 35.0%
TTAGCAAAGTTGAGTGGTAA+AGG - Chr5:5410163-5410182 MsG0580024474.01.T01:CDS 35.0%
! AAGGTGATTGATGGTAGTTA+TGG + Chr5:5409822-5409841 None:intergenic 35.0%
! TGGATTCTGGTTATATATGG+CGG - Chr5:5410227-5410246 MsG0580024474.01.T01:CDS 35.0%
AAGCATGTTGATCAACGATC+AGG - Chr5:5410406-5410425 MsG0580024474.01.T01:CDS 40.0%
AATCACCTTCCTAGAGAATC+CGG - Chr5:5409833-5409852 MsG0580024474.01.T01:CDS 40.0%
AGCATGTTGATCAACGATCA+GGG - Chr5:5410407-5410426 MsG0580024474.01.T01:CDS 40.0%
ATATGAATTCCCTTTCCCAC+CGG + Chr5:5410301-5410320 None:intergenic 40.0%
ATGAGTTAGTTGCTCTCTCT+GGG + Chr5:5409781-5409800 None:intergenic 40.0%
ATGGATAAACCAACACTCGT+TGG + Chr5:5409941-5409960 None:intergenic 40.0%
CAGGGGATGTTCACTTTATT+AGG - Chr5:5410425-5410444 MsG0580024474.01.T01:CDS 40.0%
CTCAGCTGATAGTAGAAACA+TGG - Chr5:5409897-5409916 MsG0580024474.01.T01:CDS 40.0%
TATGAGTTAGTTGCTCTCTC+TGG + Chr5:5409782-5409801 None:intergenic 40.0%
TGGATAAACCAACACTCGTT+GGG + Chr5:5409940-5409959 None:intergenic 40.0%
TGTGCATAGTTCCTGATGAA+CGG - Chr5:5409990-5410009 MsG0580024474.01.T01:CDS 40.0%
TGTTCGGTCAGTATTTCTTC+CGG - Chr5:5410279-5410298 MsG0580024474.01.T01:CDS 40.0%
TTGAGGTTAGCAAAGTTGAG+TGG - Chr5:5410157-5410176 MsG0580024474.01.T01:CDS 40.0%
! GACGTGCTGTTCATAATAGA+TGG - Chr5:5410380-5410399 MsG0580024474.01.T01:CDS 40.0%
! GTTGGTTTATCCATAGCAAG+CGG - Chr5:5409947-5409966 MsG0580024474.01.T01:CDS 40.0%
! TTCTCTAGGAAGGTGATTGA+TGG + Chr5:5409831-5409850 None:intergenic 40.0%
!! GAAGTGATGGATGGTTTGAT+TGG - Chr5:5410085-5410104 MsG0580024474.01.T01:CDS 40.0%
AAACCATCCATCACTTCCTC+CGG + Chr5:5410082-5410101 None:intergenic 45.0%
AGTATTTCTTCCGGTGGGAA+AGG - Chr5:5410288-5410307 MsG0580024474.01.T01:CDS 45.0%
GCAAAAAACATGGGAGAAGC+TGG - Chr5:5410028-5410047 MsG0580024474.01.T01:CDS 45.0%
GCATGTTGATCAACGATCAG+GGG - Chr5:5410408-5410427 MsG0580024474.01.T01:CDS 45.0%
GGTGGGAAAGGGAATTCATA+TGG - Chr5:5410300-5410319 MsG0580024474.01.T01:CDS 45.0%
GTATTTCTTCCGGTGGGAAA+GGG - Chr5:5410289-5410308 MsG0580024474.01.T01:CDS 45.0%
GTTACGGCCATGAAGCATAA+GGG - Chr5:5410358-5410377 MsG0580024474.01.T01:CDS 45.0%
TATCATCTATGGCAGCAGGA+TGG - Chr5:5409870-5409889 MsG0580024474.01.T01:CDS 45.0%
TCCTGATGAACGGTCAAGAT+CGG - Chr5:5410000-5410019 MsG0580024474.01.T01:CDS 45.0%
TGAGTTAGTTGCTCTCTCTG+GGG + Chr5:5409780-5409799 None:intergenic 45.0%
TGTTACGGCCATGAAGCATA+AGG - Chr5:5410357-5410376 MsG0580024474.01.T01:CDS 45.0%
! TGGTATGTCACCGGAAGTTT+TGG - Chr5:5410048-5410067 MsG0580024474.01.T01:CDS 45.0%
! TGGTGGTGACAACTCTGTTA+CGG - Chr5:5410342-5410361 MsG0580024474.01.T01:CDS 45.0%
!! ATGTCACCGGAAGTTTTGGT+GGG - Chr5:5410052-5410071 MsG0580024474.01.T01:CDS 45.0%
!! TATGTCACCGGAAGTTTTGG+TGG - Chr5:5410051-5410070 MsG0580024474.01.T01:CDS 45.0%
AACAGAGTTGTCACCACCAC+CGG + Chr5:5410341-5410360 None:intergenic 50.0%
AGCACGTCCCTTATGCTTCA+TGG + Chr5:5410368-5410387 None:intergenic 50.0%
CGGTCAGTATTTCTTCCGGT+GGG - Chr5:5410283-5410302 MsG0580024474.01.T01:CDS 50.0%
CTGGTATCATCTATGGCAGC+AGG - Chr5:5409866-5409885 MsG0580024474.01.T01:CDS 50.0%
GGAGTTATCCCAACGAGTGT+TGG - Chr5:5409929-5409948 MsG0580024474.01.T01:CDS 50.0%
GGTTCTCCCACCAAAACTTC+CGG + Chr5:5410061-5410080 None:intergenic 50.0%
GTAGAAACATGGTCACACGG+CGG - Chr5:5409908-5409927 MsG0580024474.01.T01:CDS 50.0%
TCCGATCTTGACCGTTCATC+AGG + Chr5:5410004-5410023 None:intergenic 50.0%
TCGGTCAGTATTTCTTCCGG+TGG - Chr5:5410282-5410301 MsG0580024474.01.T01:CDS 50.0%
! TGGTTATATATGGCGGAGCG+TGG - Chr5:5410234-5410253 MsG0580024474.01.T01:CDS 50.0%
! TTATATATGGCGGAGCGTGG+TGG - Chr5:5410237-5410256 MsG0580024474.01.T01:CDS 50.0%
!! GGAAGTTTTGGTGGGAGAAC+CGG - Chr5:5410060-5410079 MsG0580024474.01.T01:CDS 50.0%
ATAGCAAGCGGTCACACAGG+TGG - Chr5:5409959-5409978 MsG0580024474.01.T01:CDS 55.0%
CCTTCCTAGAGAATCCGGTG+CGG - Chr5:5409838-5409857 MsG0580024474.01.T01:CDS 55.0%
GGCGGAGCTGGTATCATCTA+TGG - Chr5:5409859-5409878 MsG0580024474.01.T01:CDS 55.0%
GGGAGAAGCTGGTATGTCAC+CGG - Chr5:5410039-5410058 MsG0580024474.01.T01:CDS 55.0%
TCCATAGCAAGCGGTCACAC+AGG - Chr5:5409956-5409975 MsG0580024474.01.T01:CDS 55.0%
! ACCTGTGTGACCGCTTGCTA+TGG + Chr5:5409960-5409979 None:intergenic 55.0%
! ATGGCACACTTTTCGGCTGC+CGG - Chr5:5410319-5410338 MsG0580024474.01.T01:CDS 55.0%
! GAACCGGAGGAAGTGATGGA+TGG - Chr5:5410076-5410095 MsG0580024474.01.T01:CDS 55.0%
! GGGGATCACGACGACTTGTT+CGG - Chr5:5410263-5410282 MsG0580024474.01.T01:CDS 55.0%
!! AGTTTTGGTGGGAGAACCGG+AGG - Chr5:5410063-5410082 MsG0580024474.01.T01:CDS 55.0%
GGGAGAACCGGAGGAAGTGA+TGG - Chr5:5410072-5410091 MsG0580024474.01.T01:CDS 60.0%
TCCTAGAGAATCCGGTGCGG+CGG - Chr5:5409841-5409860 MsG0580024474.01.T01:CDS 60.0%
! CCGCACCGGATTCTCTAGGA+AGG + Chr5:5409841-5409860 None:intergenic 60.0%
TCCGCCGCACCGGATTCTCT+AGG + Chr5:5409845-5409864 None:intergenic 65.0%
TGATACCAGCTCCGCCGCAC+CGG + Chr5:5409855-5409874 None:intergenic 65.0%
! CACTTTTCGGCTGCCGGTGG+TGG - Chr5:5410325-5410344 MsG0580024474.01.T01:CDS 65.0%
! GCACACTTTTCGGCTGCCGG+TGG - Chr5:5410322-5410341 MsG0580024474.01.T01:CDS 65.0%
AGAATCCGGTGCGGCGGAGC+TGG - Chr5:5409847-5409866 MsG0580024474.01.T01:CDS 70.0%
! TATGGCGGAGCGTGGTGGCG+CGG - Chr5:5410242-5410261 MsG0580024474.01.T01:CDS 70.0%
! ATGGCGGAGCGTGGTGGCGC+GGG - Chr5:5410243-5410262 MsG0580024474.01.T01:CDS 75.0%
!! TGGCGGAGCGTGGTGGCGCG+GGG - Chr5:5410244-5410263 MsG0580024474.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr5 gene 5409752 5410453 5409752 ID=MsG0580024474.01;Name=MsG0580024474.01
Chr5 mRNA 5409752 5410453 5409752 ID=MsG0580024474.01.T01;Parent=MsG0580024474.01;Name=MsG0580024474.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|233
Chr5 exon 5409752 5410453 5409752 ID=MsG0580024474.01.T01:exon:25659;Parent=MsG0580024474.01.T01
Chr5 CDS 5409752 5410453 5409752 ID=MsG0580024474.01.T01:cds;Parent=MsG0580024474.01.T01
Gene Sequence

>MsG0580024474.01.T01

ATGAAAGACATGAAACTTGTTTGGTCCCCAGAGAGAGCAACTAACTCATACATAGACACAGTTGAAGCCATAACTACCATCAATCACCTTCCTAGAGAATCCGGTGCGGCGGAGCTGGTATCATCTATGGCAGCAGGATGGAACGCTCAGCTGATAGTAGAAACATGGTCACACGGCGGAGTTATCCCAACGAGTGTTGGTTTATCCATAGCAAGCGGTCACACAGGTGGAAGACACGTGTGCATAGTTCCTGATGAACGGTCAAGATCGGAGTATGCAAAAAACATGGGAGAAGCTGGTATGTCACCGGAAGTTTTGGTGGGAGAACCGGAGGAAGTGATGGATGGTTTGATTGGAATAGATTTCTTGGTTGTTGATTCTCGGAGAAAAGATTTTACAAGAGTGTTGAGGTTAGCAAAGTTGAGTGGTAAAGGCGCTGTGTTGATATGTAAGAATGCTAATTTTATTTCTAAGATGGATTCTGGTTATATATGGCGGAGCGTGGTGGCGCGGGGATCACGACGACTTGTTCGGTCAGTATTTCTTCCGGTGGGAAAGGGAATTCATATGGCACACTTTTCGGCTGCCGGTGGTGGTGACAACTCTGTTACGGCCATGAAGCATAAGGGACGTGCTGTTCATAATAGATGGATTAAGCATGTTGATCAACGATCAGGGGATGTTCACTTTATTAGGAAATGA

Protein sequence

>MsG0580024474.01.T01

MKDMKLVWSPERATNSYIDTVEAITTINHLPRESGAAELVSSMAAGWNAQLIVETWSHGGVIPTSVGLSIASGHTGGRHVCIVPDERSRSEYAKNMGEAGMSPEVLVGEPEEVMDGLIGIDFLVVDSRRKDFTRVLRLAKLSGKGAVLICKNANFISKMDSGYIWRSVVARGSRRLVRSVFLPVGKGIHMAHFSAAGGGDNSVTAMKHKGRAVHNRWIKHVDQRSGDVHFIRK*