Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025351.01.T01 | XP_003612598.1 | 97.101 | 207 | 6 | 0 | 20 | 226 | 60 | 266 | 1.41E-139 | 401 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025351.01.T01 | Q12955 | 41.346 | 104 | 58 | 1 | 20 | 120 | 89 | 192 | 2.21E-15 | 78.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025351.01.T01 | G7K6T6 | 97.101 | 207 | 6 | 0 | 20 | 226 | 60 | 266 | 6.73e-140 | 401 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580024779.01 | MsG0580025351.01 | 0.813228 | 2.847027e-51 | 1.848474e-48 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025351.01.T01 | MTR_5g026800 | 97.101 | 207 | 6 | 0 | 20 | 226 | 60 | 266 | 1.71e-143 | 401 |
| MsG0580025351.01.T01 | MTR_5g022050 | 36.082 | 97 | 60 | 1 | 17 | 111 | 302 | 398 | 1.28e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025351.01.T01 | AT4G19150 | 51.442 | 208 | 69 | 3 | 20 | 224 | 65 | 243 | 3.53e-54 | 174 |
| MsG0580025351.01.T01 | AT4G19150 | 51.442 | 208 | 69 | 3 | 20 | 224 | 42 | 220 | 4.11e-54 | 173 |
| MsG0580025351.01.T01 | AT5G12320 | 32.653 | 98 | 66 | 0 | 41 | 138 | 47 | 144 | 1.99e-12 | 63.2 |
| MsG0580025351.01.T01 | AT5G40160 | 38.043 | 92 | 57 | 0 | 20 | 111 | 200 | 291 | 6.63e-12 | 64.3 |
| MsG0580025351.01.T01 | AT2G03430 | 33.028 | 109 | 71 | 1 | 14 | 120 | 92 | 200 | 6.57e-11 | 60.8 |
Find 61 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGATTCTTCAGGTGCTTT+TGG | 0.220900 | 5:-17324307 | None:intergenic |
| AGTGAGACTTGCCCCTTTCT+TGG | 0.250302 | 5:-17324128 | None:intergenic |
| ATAAGGAAGAATACTTTATC+TGG | 0.257058 | 5:+17323782 | MsG0580025351.01.T01:CDS |
| AAACCCTTCACAACCGAAAA+AGG | 0.281208 | 5:+17324453 | MsG0580025351.01.T01:CDS |
| TGACTGTATTCTGGGTATAT+TGG | 0.283700 | 5:+17323756 | MsG0580025351.01.T01:CDS |
| CCTACAGGGTCTCTTCTTCT+TGG | 0.284378 | 5:-17324516 | None:intergenic |
| GCCTTTGGATCAGATTCTTC+AGG | 0.317269 | 5:-17324316 | None:intergenic |
| TCTGGGTATATTGGGAAATA+AGG | 0.327127 | 5:+17323765 | MsG0580025351.01.T01:CDS |
| GACTGTATTCTGGGTATATT+GGG | 0.337986 | 5:+17323757 | MsG0580025351.01.T01:CDS |
| TGCTTCTGCTATGGATGATA+TGG | 0.344938 | 5:+17323952 | MsG0580025351.01.T01:CDS |
| CTTGGACATCATCTGAACTT+TGG | 0.372832 | 5:-17324498 | None:intergenic |
| TGCTGTTCAAGGTTCCCATC+TGG | 0.407073 | 5:+17324087 | MsG0580025351.01.T01:CDS |
| TTTAGAAGTTGTTCGAGCTC+TGG | 0.422022 | 5:+17324006 | MsG0580025351.01.T01:CDS |
| CCTAAAGAGGATGATGCCTT+TGG | 0.430235 | 5:-17324331 | None:intergenic |
| GTCAAGTACTTGTCCAAGAA+AGG | 0.437785 | 5:+17324115 | MsG0580025351.01.T01:CDS |
| GTTCGAGCTCTGGTTTCAGC+TGG | 0.438220 | 5:+17324016 | MsG0580025351.01.T01:CDS |
| CCAAAGGCATCATCCTCTTT+AGG | 0.445769 | 5:+17324331 | MsG0580025351.01.T01:CDS |
| TTCACTACTCAAATTACCTT+CGG | 0.461363 | 5:-17324356 | None:intergenic |
| CTCACTATCAAGACAAAGGC+AGG | 0.472180 | 5:+17324145 | MsG0580025351.01.T01:CDS |
| CCGAGTAGAGGCTTTGAAAG+AGG | 0.482699 | 5:-17324041 | None:intergenic |
| TGGTGAGAAAGAGAAGAGGA+AGG | 0.484213 | 5:+17324408 | MsG0580025351.01.T01:CDS |
| TCAAGTACTTGTCCAAGAAA+GGG | 0.485148 | 5:+17324116 | MsG0580025351.01.T01:CDS |
| CATTGGTAGCAAGATCCAAT+GGG | 0.488232 | 5:-17324174 | None:intergenic |
| TCTTCATCAACGGTAGCTGC+AGG | 0.489025 | 5:-17324382 | None:intergenic |
| TTTGAAAAGTCAGCTAAGAA+TGG | 0.495928 | 5:+17324223 | MsG0580025351.01.T01:CDS |
| GGGTAATGAAGATGATGAAA+GGG | 0.517277 | 5:+17324429 | MsG0580025351.01.T01:CDS |
| CCTTGAACAGCAAAATGTAA+TGG | 0.521701 | 5:-17324076 | None:intergenic |
| AAAGGCGCGGACTTCATCAT+TGG | 0.527813 | 5:-17324191 | None:intergenic |
| ATTACCTTCGGAGCCTAAAG+AGG | 0.537106 | 5:-17324344 | None:intergenic |
| TAAGGAAGAATACTTTATCT+GGG | 0.538470 | 5:+17323783 | MsG0580025351.01.T01:CDS |
| CTCACCACTTTCTTCATCAA+CGG | 0.541261 | 5:-17324392 | None:intergenic |
| AGGGTAATGAAGATGATGAA+AGG | 0.548445 | 5:+17324428 | MsG0580025351.01.T01:CDS |
| CATCATCTGAACTTTGGAGA+TGG | 0.551884 | 5:-17324492 | None:intergenic |
| TGATGTCGGTGCTTCTGCTA+TGG | 0.553962 | 5:+17323943 | MsG0580025351.01.T01:CDS |
| TCATTGGTAGCAAGATCCAA+TGG | 0.558682 | 5:-17324175 | None:intergenic |
| ACCTGAAGAATCTGATCCAA+AGG | 0.559801 | 5:+17324315 | MsG0580025351.01.T01:CDS |
| CATCAGCTTTATTCTTGCAG+AGG | 0.562565 | 5:-17323925 | None:intergenic |
| TTCAAAGCCTCTACTCGGAA+AGG | 0.573062 | 5:+17324046 | MsG0580025351.01.T01:CDS |
| AGTCACACCTTTCCGAGTAG+AGG | 0.573366 | 5:-17324053 | None:intergenic |
| CCTCTTTCAAAGCCTCTACT+CGG | 0.573614 | 5:+17324041 | MsG0580025351.01.T01:CDS |
| AAAGTGGTGAGAAAGAGAAG+AGG | 0.577048 | 5:+17324404 | MsG0580025351.01.T01:CDS |
| GGTGAGAAAGAGAAGAGGAA+GGG | 0.577937 | 5:+17324409 | MsG0580025351.01.T01:CDS |
| TCATCCTCTTTAGGCTCCGA+AGG | 0.581554 | 5:+17324340 | MsG0580025351.01.T01:CDS |
| CTGTGCCGCGAAGTGTATTG+CGG | 0.594539 | 5:-17323975 | None:intergenic |
| GAAGTCCGCGCCTTTCTAGA+GGG | 0.595781 | 5:+17324199 | MsG0580025351.01.T01:CDS |
| TATGGCCGCAATACACTTCG+CGG | 0.596559 | 5:+17323970 | MsG0580025351.01.T01:CDS |
| ATACACTTCGCGGCACAGAA+AGG | 0.600317 | 5:+17323980 | MsG0580025351.01.T01:CDS |
| CAAGTACTTGTCCAAGAAAG+GGG | 0.609645 | 5:+17324117 | MsG0580025351.01.T01:CDS |
| AAAGGCAGGAAAGACCCCAT+TGG | 0.612270 | 5:+17324159 | MsG0580025351.01.T01:CDS |
| AAGAACGAAGATAAAACTGA+AGG | 0.613960 | 5:+17324253 | MsG0580025351.01.T01:CDS |
| AGTACTTGACAAGTTCCAGA+TGG | 0.615284 | 5:-17324102 | None:intergenic |
| TGAAGTCCGCGCCTTTCTAG+AGG | 0.620823 | 5:+17324198 | MsG0580025351.01.T01:CDS |
| TCATCAACGGTAGCTGCAGG+AGG | 0.622299 | 5:-17324379 | None:intergenic |
| CAAATCCCTCTAGAAAGGCG+CGG | 0.629038 | 5:-17324204 | None:intergenic |
| TGCAAGAATAAAGCTGATGT+CGG | 0.636789 | 5:+17323929 | MsG0580025351.01.T01:CDS |
| GCTACCGTTGATGAAGAAAG+TGG | 0.655950 | 5:+17324388 | MsG0580025351.01.T01:CDS |
| GTACTTGACAAGTTCCAGAT+GGG | 0.659925 | 5:-17324101 | None:intergenic |
| AAGTCTCACTATCAAGACAA+AGG | 0.667954 | 5:+17324141 | MsG0580025351.01.T01:CDS |
| CCAAGAAGAAGAGACCCTGT+AGG | 0.691926 | 5:+17324516 | MsG0580025351.01.T01:CDS |
| TATGCCTCTACCAACCTACA+GGG | 0.721432 | 5:-17324530 | None:intergenic |
| ATTGGTAGCAAGATCCAATG+GGG | 0.757229 | 5:-17324173 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAAGGAAGAATACTTTATC+TGG | + | Chr5:17323782-17323801 | MsG0580025351.01.T01:CDS | 25.0% |
| ! | TAAGGAAGAATACTTTATCT+GGG | + | Chr5:17323783-17323802 | MsG0580025351.01.T01:CDS | 25.0% |
| ! | TGTAGTCTATTGTTCAAATT+TGG | - | Chr5:17323866-17323885 | None:intergenic | 25.0% |
| AAGAACGAAGATAAAACTGA+AGG | + | Chr5:17324253-17324272 | MsG0580025351.01.T01:CDS | 30.0% | |
| TGGATGTTAAAGAAACAACA+AGG | - | Chr5:17323846-17323865 | None:intergenic | 30.0% | |
| TTCACTACTCAAATTACCTT+CGG | - | Chr5:17324359-17324378 | None:intergenic | 30.0% | |
| TTTGAAAAGTCAGCTAAGAA+TGG | + | Chr5:17324223-17324242 | MsG0580025351.01.T01:CDS | 30.0% | |
| ! | TACATTTAGCTGCATTTTCT+GGG | + | Chr5:17323882-17323901 | MsG0580025351.01.T01:intron | 30.0% |
| ! | TTCTTCGTTTTCTTTTGGAT+CGG | - | Chr5:17324281-17324300 | None:intergenic | 30.0% |
| AAGTCTCACTATCAAGACAA+AGG | + | Chr5:17324141-17324160 | MsG0580025351.01.T01:CDS | 35.0% | |
| AGGGTAATGAAGATGATGAA+AGG | + | Chr5:17324428-17324447 | MsG0580025351.01.T01:CDS | 35.0% | |
| CCTTGAACAGCAAAATGTAA+TGG | - | Chr5:17324079-17324098 | None:intergenic | 35.0% | |
| GACTGTATTCTGGGTATATT+GGG | + | Chr5:17323757-17323776 | MsG0580025351.01.T01:CDS | 35.0% | |
| GGGTAATGAAGATGATGAAA+GGG | + | Chr5:17324429-17324448 | MsG0580025351.01.T01:CDS | 35.0% | |
| TCAAGTACTTGTCCAAGAAA+GGG | + | Chr5:17324116-17324135 | MsG0580025351.01.T01:CDS | 35.0% | |
| TCTGGGTATATTGGGAAATA+AGG | + | Chr5:17323765-17323784 | MsG0580025351.01.T01:CDS | 35.0% | |
| TGACTGTATTCTGGGTATAT+TGG | + | Chr5:17323756-17323775 | MsG0580025351.01.T01:CDS | 35.0% | |
| TGCAAGAATAAAGCTGATGT+CGG | + | Chr5:17323929-17323948 | MsG0580025351.01.T01:CDS | 35.0% | |
| ! | CGAAGTAACTGAGTTTTTCT+TGG | + | Chr5:17323806-17323825 | MsG0580025351.01.T01:intron | 35.0% |
| ! | CTACATTTAGCTGCATTTTC+TGG | + | Chr5:17323881-17323900 | MsG0580025351.01.T01:intron | 35.0% |
| ! | TCGGATTCTTCGTTTTCTTT+TGG | - | Chr5:17324286-17324305 | None:intergenic | 35.0% |
| !! | CTTTTCAAATCCCTCTAGAA+AGG | - | Chr5:17324212-17324231 | None:intergenic | 35.0% |
| !!! | CCATTACATTTTGCTGTTCA+AGG | + | Chr5:17324076-17324095 | MsG0580025351.01.T01:CDS | 35.0% |
| AAACCCTTCACAACCGAAAA+AGG | + | Chr5:17324453-17324472 | MsG0580025351.01.T01:CDS | 40.0% | |
| AAAGTGGTGAGAAAGAGAAG+AGG | + | Chr5:17324404-17324423 | MsG0580025351.01.T01:CDS | 40.0% | |
| ACCTGAAGAATCTGATCCAA+AGG | + | Chr5:17324315-17324334 | MsG0580025351.01.T01:CDS | 40.0% | |
| AGTACTTGACAAGTTCCAGA+TGG | - | Chr5:17324105-17324124 | None:intergenic | 40.0% | |
| CAAGTACTTGTCCAAGAAAG+GGG | + | Chr5:17324117-17324136 | MsG0580025351.01.T01:CDS | 40.0% | |
| CTCACCACTTTCTTCATCAA+CGG | - | Chr5:17324395-17324414 | None:intergenic | 40.0% | |
| CTTGGACATCATCTGAACTT+TGG | - | Chr5:17324501-17324520 | None:intergenic | 40.0% | |
| GTACTTGACAAGTTCCAGAT+GGG | - | Chr5:17324104-17324123 | None:intergenic | 40.0% | |
| GTCAAGTACTTGTCCAAGAA+AGG | + | Chr5:17324115-17324134 | MsG0580025351.01.T01:CDS | 40.0% | |
| TGCTTCTGCTATGGATGATA+TGG | + | Chr5:17323952-17323971 | MsG0580025351.01.T01:CDS | 40.0% | |
| TTTAGAAGTTGTTCGAGCTC+TGG | + | Chr5:17324006-17324025 | MsG0580025351.01.T01:CDS | 40.0% | |
| ! | ATTGGTAGCAAGATCCAATG+GGG | - | Chr5:17324176-17324195 | None:intergenic | 40.0% |
| ! | CATCATCTGAACTTTGGAGA+TGG | - | Chr5:17324495-17324514 | None:intergenic | 40.0% |
| ! | CATTGGTAGCAAGATCCAAT+GGG | - | Chr5:17324177-17324196 | None:intergenic | 40.0% |
| ! | TCAGATTCTTCAGGTGCTTT+TGG | - | Chr5:17324310-17324329 | None:intergenic | 40.0% |
| ! | TCATTGGTAGCAAGATCCAA+TGG | - | Chr5:17324178-17324197 | None:intergenic | 40.0% |
| ! | TTCTTCAGGTGCTTTTGGAT+CGG | - | Chr5:17324305-17324324 | None:intergenic | 40.0% |
| !! | CATCAGCTTTATTCTTGCAG+AGG | - | Chr5:17323928-17323947 | None:intergenic | 40.0% |
| ATTACCTTCGGAGCCTAAAG+AGG | - | Chr5:17324347-17324366 | None:intergenic | 45.0% | |
| CCTAAAGAGGATGATGCCTT+TGG | - | Chr5:17324334-17324353 | None:intergenic | 45.0% | |
| CCTCTTTCAAAGCCTCTACT+CGG | + | Chr5:17324041-17324060 | MsG0580025351.01.T01:CDS | 45.0% | |
| CTCACTATCAAGACAAAGGC+AGG | + | Chr5:17324145-17324164 | MsG0580025351.01.T01:CDS | 45.0% | |
| GCCTTTGGATCAGATTCTTC+AGG | - | Chr5:17324319-17324338 | None:intergenic | 45.0% | |
| GGTGAGAAAGAGAAGAGGAA+GGG | + | Chr5:17324409-17324428 | MsG0580025351.01.T01:CDS | 45.0% | |
| TGGTGAGAAAGAGAAGAGGA+AGG | + | Chr5:17324408-17324427 | MsG0580025351.01.T01:CDS | 45.0% | |
| TTCAAAGCCTCTACTCGGAA+AGG | + | Chr5:17324046-17324065 | MsG0580025351.01.T01:CDS | 45.0% | |
| ! | AGCTGAACTCTCGCCTTTTT+CGG | - | Chr5:17324469-17324488 | None:intergenic | 45.0% |
| ! | GCTACCGTTGATGAAGAAAG+TGG | + | Chr5:17324388-17324407 | MsG0580025351.01.T01:CDS | 45.0% |
| !! | CCAAAGGCATCATCCTCTTT+AGG | + | Chr5:17324331-17324350 | MsG0580025351.01.T01:CDS | 45.0% |
| !!! | TCGCCTTTTTCGGTTGTGAA+GGG | - | Chr5:17324459-17324478 | None:intergenic | 45.0% |
| AAAGGCAGGAAAGACCCCAT+TGG | + | Chr5:17324159-17324178 | MsG0580025351.01.T01:CDS | 50.0% | |
| AGTCACACCTTTCCGAGTAG+AGG | - | Chr5:17324056-17324075 | None:intergenic | 50.0% | |
| AGTGAGACTTGCCCCTTTCT+TGG | - | Chr5:17324131-17324150 | None:intergenic | 50.0% | |
| ATACACTTCGCGGCACAGAA+AGG | + | Chr5:17323980-17323999 | MsG0580025351.01.T01:CDS | 50.0% | |
| TATGGCCGCAATACACTTCG+CGG | + | Chr5:17323970-17323989 | MsG0580025351.01.T01:CDS | 50.0% | |
| TCATCCTCTTTAGGCTCCGA+AGG | + | Chr5:17324340-17324359 | MsG0580025351.01.T01:CDS | 50.0% | |
| TCTTCATCAACGGTAGCTGC+AGG | - | Chr5:17324385-17324404 | None:intergenic | 50.0% | |
| TGCTGTTCAAGGTTCCCATC+TGG | + | Chr5:17324087-17324106 | MsG0580025351.01.T01:CDS | 50.0% | |
| ! | AAAGGCGCGGACTTCATCAT+TGG | - | Chr5:17324194-17324213 | None:intergenic | 50.0% |
| ! | CAAATCCCTCTAGAAAGGCG+CGG | - | Chr5:17324207-17324226 | None:intergenic | 50.0% |
| ! | TGATGTCGGTGCTTCTGCTA+TGG | + | Chr5:17323943-17323962 | MsG0580025351.01.T01:CDS | 50.0% |
| !! | CCGAGTAGAGGCTTTGAAAG+AGG | - | Chr5:17324044-17324063 | None:intergenic | 50.0% |
| !!! | CTCGCCTTTTTCGGTTGTGA+AGG | - | Chr5:17324460-17324479 | None:intergenic | 50.0% |
| CTGTGCCGCGAAGTGTATTG+CGG | - | Chr5:17323978-17323997 | None:intergenic | 55.0% | |
| GAAGTCCGCGCCTTTCTAGA+GGG | + | Chr5:17324199-17324218 | MsG0580025351.01.T01:CDS | 55.0% | |
| TCATCAACGGTAGCTGCAGG+AGG | - | Chr5:17324382-17324401 | None:intergenic | 55.0% | |
| TGAAGTCCGCGCCTTTCTAG+AGG | + | Chr5:17324198-17324217 | MsG0580025351.01.T01:CDS | 55.0% | |
| ! | GTTCGAGCTCTGGTTTCAGC+TGG | + | Chr5:17324016-17324035 | MsG0580025351.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 17323755 | 17324537 | 17323755 | ID=MsG0580025351.01;Name=MsG0580025351.01 |
| Chr5 | mRNA | 17323755 | 17324537 | 17323755 | ID=MsG0580025351.01.T01;Parent=MsG0580025351.01;Name=MsG0580025351.01.T01;_AED=0.49;_eAED=0.54;_QI=0|0|0|1|1|1|2|0|226 |
| Chr5 | exon | 17323755 | 17323809 | 17323755 | ID=MsG0580025351.01.T01:exon:22838;Parent=MsG0580025351.01.T01 |
| Chr5 | exon | 17323912 | 17324537 | 17323912 | ID=MsG0580025351.01.T01:exon:22839;Parent=MsG0580025351.01.T01 |
| Chr5 | CDS | 17323755 | 17323809 | 17323755 | ID=MsG0580025351.01.T01:cds;Parent=MsG0580025351.01.T01 |
| Chr5 | CDS | 17323912 | 17324537 | 17323912 | ID=MsG0580025351.01.T01:cds;Parent=MsG0580025351.01.T01 |
| Gene Sequence |
| Protein sequence |
