AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026075.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026075.01.T01 MTR_5g039000 91.209 91 7 1 2 91 58 148 1.47e-52 166
MsG0580026075.01.T01 MTR_8g468210 89.412 85 8 1 8 91 64 148 6.02e-48 154
MsG0580026075.01.T01 MTR_6g011610 73.563 87 20 2 8 91 62 148 1.67e-37 126
MsG0580026075.01.T01 MTR_6g011610 73.563 87 20 2 8 91 81 167 1.99e-37 127
MsG0580026075.01.T01 MTR_7g010020 66.279 86 27 2 8 91 66 151 9.06e-34 117
MsG0580026075.01.T01 MTR_6g007647 72.368 76 20 1 17 91 29 104 8.67e-31 109
MsG0580026075.01.T01 MTR_3g080100 63.636 88 31 1 5 91 4 91 1.12e-30 107
MsG0580026075.01.T01 MTR_3g080100 63.636 88 31 1 5 91 43 130 2.33e-30 108
MsG0580026075.01.T01 MTR_6g007647 72.368 76 20 1 17 91 63 138 2.61e-30 108
MsG0580026075.01.T01 MTR_8g089895 63.953 86 28 2 9 91 40 125 3.66e-30 108
MsG0580026075.01.T01 MTR_3g086790 64.706 85 29 1 8 91 47 131 2.13e-29 106
MsG0580026075.01.T01 MTR_4g107650 64.286 84 29 1 9 91 83 166 2.22e-27 102
MsG0580026075.01.T01 MTR_8g469430 69.737 76 21 2 17 91 86 160 7.43e-27 99.8
MsG0580026075.01.T01 MTR_5g038280 69.444 72 21 1 21 91 87 158 1.05e-26 100
MsG0580026075.01.T01 MTR_7g103340 53.488 86 39 1 9 93 51 136 1.66e-25 95.1
MsG0580026075.01.T01 MTR_1g061660 57.333 75 31 1 20 93 58 132 4.59e-23 88.6
MsG0580026075.01.T01 MTR_3g092150 59.155 71 28 1 23 92 40 110 4.11e-21 84.0
MsG0580026075.01.T01 MTR_8g026960 57.576 66 27 1 28 92 41 106 3.58e-20 81.3
MsG0580026075.01.T01 MTR_4g097600 47.191 89 44 2 5 92 113 199 4.46e-17 73.6
MsG0580026075.01.T01 MTR_5g019680 54.795 73 30 2 21 91 53 124 5.91e-16 69.7
MsG0580026075.01.T01 MTR_5g019650 54.795 73 30 2 21 91 53 124 1.26e-15 68.9
MsG0580026075.01.T01 MTR_4g100550 45.349 86 45 2 9 92 107 192 7.40e-15 67.8
MsG0580026075.01.T01 MTR_5g014890 46.914 81 42 1 13 92 115 195 1.25e-14 67.0
MsG0580026075.01.T01 MTR_2g061030 42.857 91 50 2 3 92 183 272 2.47e-14 66.6
MsG0580026075.01.T01 MTR_1g054285 45.679 81 43 1 13 92 146 226 5.23e-14 65.5
MsG0580026075.01.T01 MTR_7g093430 44.444 81 44 1 13 92 152 232 1.02e-13 64.7
MsG0580026075.01.T01 MTR_8g006705 50.685 73 35 1 21 92 146 218 1.29e-13 64.7
MsG0580026075.01.T01 MTR_1g017090 49.315 73 36 1 21 92 130 202 2.51e-13 63.5
MsG0580026075.01.T01 MTR_4g126900 47.143 70 36 1 24 92 91 160 2.90e-13 62.8
MsG0580026075.01.T01 MTR_3g103590 43.478 92 51 1 2 92 126 217 7.12e-13 62.4
MsG0580026075.01.T01 MTR_5g013010 41.975 81 46 1 13 92 128 208 2.70e-11 58.2
MsG0580026075.01.T01 MTR_4g084750 46.575 73 38 1 21 92 65 137 3.57e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026075.01.T01 AT1G69780 85.882 85 11 1 8 91 72 156 3.08e-46 150
MsG0580026075.01.T01 AT5G15150 71.277 94 23 3 2 91 93 186 1.30e-39 133
MsG0580026075.01.T01 AT1G26960 73.256 86 22 1 7 91 57 142 6.14e-38 127
MsG0580026075.01.T01 AT3G01220 65.591 93 29 2 2 91 66 158 2.35e-37 127
MsG0580026075.01.T01 AT5G65310 68.132 91 25 2 7 93 37 127 1.42e-32 114
MsG0580026075.01.T01 AT5G65310 68.132 91 25 2 7 93 55 145 1.74e-32 115
MsG0580026075.01.T01 AT4G40060 75.342 73 17 1 20 91 58 130 9.50e-31 110
MsG0580026075.01.T01 AT2G22430 75.342 73 17 1 20 91 61 133 9.77e-31 110
MsG0580026075.01.T01 AT3G01470 71.053 76 21 1 17 91 64 139 1.52e-29 106
MsG0580026075.01.T01 AT2G46680 56.944 72 30 1 22 92 33 104 3.96e-22 86.7
MsG0580026075.01.T01 AT2G46680 56.944 72 30 1 22 92 33 104 4.09e-22 86.7
MsG0580026075.01.T01 AT3G61890 57.143 70 29 1 24 92 33 102 6.49e-21 83.2
MsG0580026075.01.T01 AT5G03790 54.054 74 33 1 21 93 77 150 3.03e-20 80.5
MsG0580026075.01.T01 AT5G03790 54.054 74 33 1 21 93 77 150 3.66e-20 81.3
MsG0580026075.01.T01 AT2G36610 45.263 95 41 3 8 93 60 152 1.68e-18 75.9
MsG0580026075.01.T01 AT1G27045 59.420 69 27 1 21 88 17 85 1.91e-18 75.5
MsG0580026075.01.T01 AT1G27045 59.420 69 27 1 21 88 25 93 1.91e-18 75.9
MsG0580026075.01.T01 AT1G27045 59.420 69 27 1 21 88 25 93 1.91e-18 75.9
MsG0580026075.01.T01 AT4G36740 49.451 91 43 2 3 91 38 127 2.46e-18 76.3
MsG0580026075.01.T01 AT4G36740 49.451 91 43 2 3 91 37 126 2.69e-18 75.9
MsG0580026075.01.T01 AT1G27045 59.420 69 27 1 21 88 68 136 3.09e-18 76.3
MsG0580026075.01.T01 AT5G66700 49.425 87 39 3 7 91 59 142 3.80e-18 75.9
MsG0580026075.01.T01 AT1G27045 59.420 69 27 1 21 88 68 136 5.43e-18 75.9
MsG0580026075.01.T01 AT2G18550 48.810 84 39 2 11 90 48 131 1.25e-16 71.6
MsG0580026075.01.T01 AT5G53980 47.368 76 39 1 16 90 6 81 3.16e-16 69.3
MsG0580026075.01.T01 AT3G60390 46.512 86 45 1 8 92 149 234 9.01e-15 67.8
MsG0580026075.01.T01 AT4G37790 43.023 86 48 1 8 92 113 198 2.79e-14 66.2
MsG0580026075.01.T01 AT5G06710 43.820 89 48 2 5 92 175 262 6.02e-14 65.9
MsG0580026075.01.T01 AT2G01430 44.186 86 47 1 8 92 126 211 7.18e-14 65.1
MsG0580026075.01.T01 AT5G47370 48.718 78 39 1 10 86 119 196 8.87e-14 65.1
MsG0580026075.01.T01 AT2G22800 44.444 81 44 1 13 92 105 185 1.10e-12 62.0
MsG0580026075.01.T01 AT4G17460 43.210 81 45 1 13 92 127 207 2.78e-12 60.8
MsG0580026075.01.T01 AT2G44910 47.945 73 37 1 21 92 163 235 3.85e-12 60.5
MsG0580026075.01.T01 AT2G01430 48.485 66 33 1 8 72 126 191 4.04e-11 57.0

Find 13 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAAGAGGAGGCTTAATA+TGG 0.330137 5:-30220810 MsG0580026075.01.T01:CDS
AACCTAGACAAGTTGCTATA+TGG 0.331625 5:-30220700 MsG0580026075.01.T01:CDS
CTATATGGTTTCAAAATAGA+AGG 0.336540 5:-30220685 MsG0580026075.01.T01:CDS
TTCAAAATAGAAGGGCTAGA+TGG 0.455440 5:-30220676 MsG0580026075.01.T01:CDS
GAAGAAGATTTATCAGATGA+TGG 0.488868 5:-30220848 MsG0580026075.01.T01:CDS
AACTTGAAAGAGTTTGAACT+TGG 0.505947 5:-30220776 MsG0580026075.01.T01:CDS
TCAGATGATGGATCACAAGC+TGG 0.546396 5:-30220836 MsG0580026075.01.T01:CDS
CACAAGCTGGAGAGAAGAAG+AGG 0.557400 5:-30220823 MsG0580026075.01.T01:CDS
AACCATATAGCAACTTGTCT+AGG 0.569003 5:+30220698 None:intergenic
TATATGGTTTCAAAATAGAA+GGG 0.590755 5:-30220684 MsG0580026075.01.T01:CDS
GTAATAAGCTTGAGCCTGAG+AGG 0.611232 5:-30220754 MsG0580026075.01.T01:CDS
AAGCTGGAGAGAAGAAGAGG+AGG 0.613532 5:-30220820 MsG0580026075.01.T01:CDS
TAGATGGAAGACAAAGCAGT+TGG 0.627956 5:-30220660 MsG0580026075.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATGGTTTCAAAATAGAA+GGG - Chr5:30220795-30220814 MsG0580026075.01.T01:CDS 20.0%
! CTATATGGTTTCAAAATAGA+AGG - Chr5:30220794-30220813 MsG0580026075.01.T01:CDS 25.0%
AACTTGAAAGAGTTTGAACT+TGG - Chr5:30220703-30220722 MsG0580026075.01.T01:CDS 30.0%
GAAGAAGATTTATCAGATGA+TGG - Chr5:30220631-30220650 MsG0580026075.01.T01:CDS 30.0%
AACCATATAGCAACTTGTCT+AGG + Chr5:30220784-30220803 None:intergenic 35.0%
AACCTAGACAAGTTGCTATA+TGG - Chr5:30220779-30220798 MsG0580026075.01.T01:CDS 35.0%
TTCAAAATAGAAGGGCTAGA+TGG - Chr5:30220803-30220822 MsG0580026075.01.T01:CDS 35.0%
GAAGAAGAGGAGGCTTAATA+TGG - Chr5:30220669-30220688 MsG0580026075.01.T01:CDS 40.0%
TAGATGGAAGACAAAGCAGT+TGG - Chr5:30220819-30220838 MsG0580026075.01.T01:CDS 40.0%
GTAATAAGCTTGAGCCTGAG+AGG - Chr5:30220725-30220744 MsG0580026075.01.T01:CDS 45.0%
!! TCAGATGATGGATCACAAGC+TGG - Chr5:30220643-30220662 MsG0580026075.01.T01:CDS 45.0%
AAGCTGGAGAGAAGAAGAGG+AGG - Chr5:30220659-30220678 MsG0580026075.01.T01:CDS 50.0%
CACAAGCTGGAGAGAAGAAG+AGG - Chr5:30220656-30220675 MsG0580026075.01.T01:CDS 50.0%
! GCAAGCTGCATTTTCCTCTC+AGG + Chr5:30220742-30220761 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 30220610 30220891 30220610 ID=MsG0580026075.01;Name=MsG0580026075.01
Chr5 mRNA 30220610 30220891 30220610 ID=MsG0580026075.01.T01;Parent=MsG0580026075.01;Name=MsG0580026075.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|93
Chr5 exon 30220610 30220891 30220610 ID=MsG0580026075.01.T01:exon:7274;Parent=MsG0580026075.01.T01
Chr5 CDS 30220610 30220891 30220610 ID=MsG0580026075.01.T01:cds;Parent=MsG0580026075.01.T01
Gene Sequence

>MsG0580026075.01.T01

CTGGAGAAGAAGAAAATCTCTGAAGAAGATTTATCAGATGATGGATCACAAGCTGGAGAGAAGAAGAGGAGGCTTAATATGGAACAAGTGAAGAACTTGAAAGAGTTTGAACTTGGTAATAAGCTTGAGCCTGAGAGGAAAATGCAGCTTGCAAGAGCTCTTAACTTGCAACCTAGACAAGTTGCTATATGGTTTCAAAATAGAAGGGCTAGATGGAAGACAAAGCAGTTGGAAAAAGACTATGATGTTCGTAAAAGACAATATGATGCTATAAGTAGATAA

Protein sequence

>MsG0580026075.01.T01

LEKKKISEEDLSDDGSQAGEKKRRLNMEQVKNLKEFELGNKLEPERKMQLARALNLQPRQVAIWFQNRRARWKTKQLEKDYDVRKRQYDAISR*