AlfalfaGEDB | MsG0580028630.01

M. sativa cultivar ZhongmuNo.1 / MsG0580028630.01

KEGG: -
GO: GO:0005515 GO:0008270
PFAM: PF01535 PF13041 PF14432

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028630.01.T01	XP_003614059.1	90.297	505	25	1	2	482	61	565	0	941

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028630.01.T01	Q0WQW5	60.078	511	169	6	4	482	131	638	0	644

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048065.01	MsG0580028630.01	0.851544	8.285354e-61	1.682728e-57
MsG0080048152.01	MsG0580028630.01	0.846651	1.899570e-59	3.292459e-56
MsG0080048727.01	MsG0580028630.01	0.846587	1.978031e-59	3.421095e-56
MsG0080048761.01	MsG0580028630.01	-0.810995	8.733699e-51	5.344067e-48
MsG0080048773.01	MsG0580028630.01	0.800587	1.343769e-48	6.280691e-46
MsG0080048817.01	MsG0580028630.01	0.800370	1.488116e-48	6.917270e-46
MsG0080048826.01	MsG0580028630.01	0.812735	3.649744e-51	2.338856e-48
MsG0080048849.01	MsG0580028630.01	0.850241	1.928909e-60	3.754426e-57
MsG0080048865.01	MsG0580028630.01	0.818705	1.704626e-52	1.285387e-49
MsG0080048878.01	MsG0580028630.01	0.883993	2.801721e-71	1.816565e-67

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PPI

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Gene1	Gene2	Type
MsG0580028630.01	MsG0080048865.01	PPI
MsG0580028630.01	MsG0780041221.01	PPI
MsG0580028630.01	MsG0380016485.01	PPI
MsG0580028630.01	MsG0780036752.01	PPI
MsG0580028630.01	MsG0580025411.01	PPI
MsG0580028630.01	MsG0580026175.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028630.01.T01	MTR_5g044260	90.297	505	25	1	2	482	61	565	0.0	941
MsG0580028630.01.T01	MTR_5g044260	28.696	230	126	7	86	283	39	262	2.30e-13	72.8
MsG0580028630.01.T01	MTR_2g018870	44.008	484	238	8	24	482	138	613	3.88e-134	401
MsG0580028630.01.T01	MTR_2g018870	27.083	288	167	9	30	283	36	314	5.59e-20	93.6
MsG0580028630.01.T01	MTR_4g113830	43.265	490	247	8	18	482	255	738	1.13e-131	398
MsG0580028630.01.T01	MTR_4g113830	28.148	405	210	13	24	361	129	519	5.77e-38	147
MsG0580028630.01.T01	MTR_4g113830	23.704	270	175	8	25	286	27	273	9.02e-12	67.8
MsG0580028630.01.T01	MTR_5g040920	42.857	476	238	8	33	482	119	586	2.15e-126	380
MsG0580028630.01.T01	MTR_5g040920	27.111	225	154	6	5	226	195	412	2.39e-16	82.0
MsG0580028630.01.T01	MTR_5g040920	22.222	315	190	10	18	302	19	308	1.29e-15	79.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028630.01.T01	AT1G59720	60.078	511	169	6	4	482	131	638	0.0	644
MsG0580028630.01.T01	AT1G08070	42.942	503	251	10	6	482	145	637	1.90e-134	402
MsG0580028630.01.T01	AT1G08070	34.528	307	137	6	22	270	26	326	4.29e-45	167
MsG0580028630.01.T01	AT1G08070	42.942	503	251	10	6	482	249	741	6.85e-133	402
MsG0580028630.01.T01	AT1G08070	34.528	307	137	6	22	270	130	430	1.41e-44	167
MsG0580028630.01.T01	AT1G08070	26.578	301	183	11	32	320	39	313	2.07e-17	85.9
MsG0580028630.01.T01	AT2G02980	41.429	490	253	8	19	482	122	603	1.40e-132	396
MsG0580028630.01.T01	AT2G02980	24.554	224	161	5	5	226	212	429	1.06e-11	67.8
MsG0580028630.01.T01	AT4G37380	39.739	536	252	10	4	482	111	632	2.03e-130	392
MsG0580028630.01.T01	AT4G21065	41.889	487	245	9	24	482	119	595	1.36e-128	386

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Find 84 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCACATTCAGAGCCGCT+TGG	0.554903	5:-83807818	None:intergenic
AAACCCAAGTTTCAAAACAT+GGG	0.639929	5:-83807117	None:intergenic
AAAGGCGGAATGTGGTTTCG+TGG	0.588057	5:+83807234	MsG0580028630.01.T01:CDS
AAAGGGTAAGTGTGTTTATC+AGG	0.341488	5:-83807038	None:intergenic
AAAGTTGCACCTGAATAGTC+AGG	0.509908	5:-83808212	None:intergenic
AACACTCTTATTGGCATGCC+TGG	0.410628	5:-83808323	None:intergenic
AACCAGATGCTGTAATTTGG+AGG	0.525315	5:+83807856	MsG0580028630.01.T01:CDS
AAGGGAAACAGAATACTATG+AGG	0.635946	5:+83808252	MsG0580028630.01.T01:CDS
AAGGGTGTGAGTAAAGAGCC+TGG	0.656105	5:+83808059	MsG0580028630.01.T01:CDS
AAGTGTATGCTTCAGCTAGT+AGG	0.504656	5:+83807997	MsG0580028630.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACCCAAGTTTCAAAACAT+GGG	-	Chr5:83807120-83807139	None:intergenic	30.0%
ACCAACAAAGTAATCGAATT+CGG	-	Chr5:83807659-83807678	None:intergenic	30.0%
AGGAGAAACTAGAATCTATA+GGG	+	Chr5:83808183-83808202	MsG0580028630.01.T01:CDS	30.0%
AGTGTGTAATGATTGTCATA+AGG	+	Chr5:83808358-83808377	MsG0580028630.01.T01:CDS	30.0%
ATCTTTACTTTGTGGATGAT+TGG	-	Chr5:83808138-83808157	None:intergenic	30.0%
ATTCATTTCTATGCTTCATG+TGG	+	Chr5:83807170-83807189	MsG0580028630.01.T01:CDS	30.0%
CGTAAGCTAATGAATGATAA+GGG	+	Chr5:83808041-83808060	MsG0580028630.01.T01:CDS	30.0%
TCGTAAGCTAATGAATGATA+AGG	+	Chr5:83808040-83808059	MsG0580028630.01.T01:CDS	30.0%
TGTTTGATAGAATGTGTGAA+AGG	+	Chr5:83807216-83807235	MsG0580028630.01.T01:CDS	30.0%
TTAAACCAGATGCTGTAATT+TGG	+	Chr5:83807853-83807872	MsG0580028630.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	83806969	83808487	83806969	ID=MsG0580028630.01;Name=MsG0580028630.01
Chr5	mRNA	83806969	83808487	83806969	ID=MsG0580028630.01.T01;Parent=MsG0580028630.01;Name=MsG0580028630.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|0\|0\|1\|1\|1\|2\|0\|482
Chr5	exon	83806969	83807676	83806969	ID=MsG0580028630.01.T01:exon:17052;Parent=MsG0580028630.01.T01
Chr5	exon	83807747	83808487	83807747	ID=MsG0580028630.01.T01:exon:17053;Parent=MsG0580028630.01.T01
Chr5	CDS	83806969	83807676	83806969	ID=MsG0580028630.01.T01:cds;Parent=MsG0580028630.01.T01
Chr5	CDS	83807747	83808487	83807747	ID=MsG0580028630.01.T01:cds;Parent=MsG0580028630.01.T01

Gene Sequence

>MsG0580028630.01.T01

ATGCTTCACAAACACAAAGCTATTCTTCTTTACAAAGCAATCATAACAGAACAAGAAAATGAACTCTTTCCTGATAAACACACTTACCCTTTTGTTCTTAAAGCTTGTGCTTACTTGTTTTCGTTCTTCGAAGGAAAACAGGTTCATGCCCATGTTTTGAAACTTGGGTTTGAGTTAGATACTTATATTTGTAACAGTTTGATTCATTTCTATGCTTCATGTGGGTGTTTGGAAACGGCGCGTAAAGTGTTTGATAGAATGTGTGAAAGGCGGAATGTGGTTTCGTGGAATGTAATGATTGATTCTTACGCGAAAGTTGGAGAGTATGATACTGTTTTGAATATGTTTTGTGAGATGATGAAGGAGTATGAACCTGATTGTTACACGATGCAGAGTGTTATTAGGGCTTGTGAGGGATTAGGTTCGCTTTCGTTGGGTATGTGGGTTCATGCTTTTGTTTTGAAAAAGTGCGATAAGAATGTGGTTTGTGCCGTTTTGGTTAATACTTGTTTGGTTGATATGTATTGTAAATGTGGATCGTTGGAAATTGCTAAGCAGGTTTTTGAAGGAATGTCTTATCGAGATGTGGGTTCATGGAATTCGATTATTTTAGGTTTTGCCATGCATGGGAAAGCTGAGGCAGCATTGGATTATTTTACCCGAATGGTGAAGGTAGAGAAAATTGTGCCGAATTCGATTACTTTGTTGGAGTACAATGTTGAACCAAGTTTAGTGCATTATGGATGTCTTGTTGATCTTTACGCTAGAGCTGGACATATCCAAGCGGCTCTGAATGTGGTTTCAGAAATGCCGATTAAACCAGATGCTGTAATTTGGAGGAGTCTTTTAGATGCTTGTTATAAGCAACATGCAAGTGTTGAGCTAAGTGAAGAATTGGCAAAGCAAATTTTTGAGTCAAATGGGAGTGTTTGTGGTGGTGCTTATGTTCTTTTGTCAAAAGTGTATGCTTCAGCTAGTAGGTGGAATGATGTAGGGTTGCTTCGTAAGCTAATGAATGATAAGGGTGTGAGTAAAGAGCCTGGTTGTAGTTTGATAGAGATAAATGGTGCTGCTCATGAATTTTTTGCTGGAGACACCAATCATCCACAAAGTAAAGATATATATAAGTTTATGAATGAAATTCAGGAGAAACTAGAATCTATAGGGTATGTACCTGACTATTCAGGTGCAACTTTTATTGATGAGATTAATGAAGGGAAACAGAATACTATGAGGTTGCATAGTGAGAGATTGGCCATAGCTTTTGGGCTTTTGAATTCAAAACCAGGCATGCCAATAAGAGTGTTTAAGAATCTTAGAGTGTGTAATGATTGTCATAAGGTTACTAAATTAATATCTAGGATTTACAATGTGGAGATAATTGTAAGAGATCGTGTTCGATTTCATCACTTTAAAGATGGGAGTTGTTCTTGTGTGGATTATTGGTGA

Protein sequence

>MsG0580028630.01.T01

MLHKHKAILLYKAIITEQENELFPDKHTYPFVLKACAYLFSFFEGKQVHAHVLKLGFELDTYICNSLIHFYASCGCLETARKVFDRMCERRNVVSWNVMIDSYAKVGEYDTVLNMFCEMMKEYEPDCYTMQSVIRACEGLGSLSLGMWVHAFVLKKCDKNVVCAVLVNTCLVDMYCKCGSLEIAKQVFEGMSYRDVGSWNSIILGFAMHGKAEAALDYFTRMVKVEKIVPNSITLLEYNVEPSLVHYGCLVDLYARAGHIQAALNVVSEMPIKPDAVIWRSLLDACYKQHASVELSEELAKQIFESNGSVCGGAYVLLSKVYASASRWNDVGLLRKLMNDKGVSKEPGCSLIEINGAAHEFFAGDTNHPQSKDIYKFMNEIQEKLESIGYVPDYSGATFIDEINEGKQNTMRLHSERLAIAFGLLNSKPGMPIRVFKNLRVCNDCHKVTKLISRIYNVEIIVRDRVRFHHFKDGSCSCVDYW*