AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048152.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATAATTTGAATATTTAT+TGG 0.149686 contig223end:+17735 None:intergenic
AATGCAAAGGGTAGAGTATA+TGG 0.262241 contig223end:-17805 MsG0080048152.01.T01:CDS
TGAATATTTATTGGATTTAG+AGG 0.288680 contig223end:+17744 None:intergenic
ACAAACAATTAATTCCATTA+TGG 0.295490 contig223end:-17647 MsG0080048152.01.T01:CDS
TAAACATGCAAATATAGATT+TGG 0.299235 contig223end:-17593 MsG0080048152.01.T01:CDS
AAATATAGATTTGGATTCAA+TGG 0.392319 contig223end:-17584 MsG0080048152.01.T01:CDS
TAGAGTATATGGGCTTGGTT+TGG 0.416967 contig223end:-17794 MsG0080048152.01.T01:CDS
ATTAGTGTATCATAATCAAT+AGG 0.448901 contig223end:+17850 None:intergenic
AAGGGTAGAGTATATGGGCT+TGG 0.493623 contig223end:-17799 MsG0080048152.01.T01:CDS
TGGTATGAGTGGAGAGGTTG+AGG 0.504275 contig223end:-17701 MsG0080048152.01.T01:CDS
TGTATCATAATCAATAGGCT+TGG 0.504722 contig223end:+17855 None:intergenic
GAGAGGTTGAGGCGCGATCA+AGG 0.508328 contig223end:-17690 MsG0080048152.01.T01:CDS
GTATCATAATCAATAGGCTT+GGG 0.509867 contig223end:+17856 None:intergenic
GTATATGGGCTTGGTTTGGA+TGG 0.514998 contig223end:-17790 MsG0080048152.01.T01:CDS
GGATTTAGAGGTTGAGCCAC+GGG 0.516888 contig223end:+17756 None:intergenic
ATGCAAAGGGTAGAGTATAT+GGG 0.526831 contig223end:-17804 MsG0080048152.01.T01:CDS
ACACTAATGATTGAAGCTGT+TGG 0.537396 contig223end:-17835 MsG0080048152.01.T01:CDS
GATGGCAGCATGCGCACCCG+TGG 0.550034 contig223end:-17772 MsG0080048152.01.T01:CDS
CTAATGATTGAAGCTGTTGG+AGG 0.555236 contig223end:-17832 MsG0080048152.01.T01:CDS
TGCAAATGTTGGTGCTGATG+TGG 0.559498 contig223end:-17512 MsG0080048152.01.T01:CDS
AATTATACTGATATTGCTCG+TGG 0.592386 contig223end:-17721 MsG0080048152.01.T01:CDS
CGTGGAAGCAATGCAAATGT+TGG 0.616233 contig223end:-17523 MsG0080048152.01.T01:CDS
CTGAGGCTGGTAATAATGAG+TGG 0.621292 contig223end:-17886 None:intergenic
GATATTGCTCGTGGTATGAG+TGG 0.631987 contig223end:-17712 MsG0080048152.01.T01:CDS
TGTTGGAGGAAGAAATGCAA+AGG 0.632509 contig223end:-17818 MsG0080048152.01.T01:CDS
TCTGTTGATAATGCTACTCG+TGG 0.635589 contig223end:-17541 MsG0080048152.01.T01:CDS
GTTGGAGGAAGAAATGCAAA+GGG 0.639504 contig223end:-17817 MsG0080048152.01.T01:CDS
TGGATTTAGAGGTTGAGCCA+CGG 0.650650 contig223end:+17755 None:intergenic
TGCTCGTGGTATGAGTGGAG+AGG 0.702619 contig223end:-17707 MsG0080048152.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTATAATTTGAATATTTAT+TGG + contig223end:17643-17662 None:intergenic 10.0%
!! AAATATAGATTTGGATTCAA+TGG - contig223end:17791-17810 MsG0080048152.01.T01:CDS 20.0%
!! ACAAACAATTAATTCCATTA+TGG - contig223end:17728-17747 MsG0080048152.01.T01:CDS 20.0%
!! ATTAGTGTATCATAATCAAT+AGG + contig223end:17528-17547 None:intergenic 20.0%
!! TAAACATGCAAATATAGATT+TGG - contig223end:17782-17801 MsG0080048152.01.T01:CDS 20.0%
!! TGAATATTTATTGGATTTAG+AGG + contig223end:17634-17653 None:intergenic 20.0%
AATTATACTGATATTGCTCG+TGG - contig223end:17654-17673 MsG0080048152.01.T01:CDS 30.0%
GTATCATAATCAATAGGCTT+GGG + contig223end:17522-17541 None:intergenic 30.0%
TGTATCATAATCAATAGGCT+TGG + contig223end:17523-17542 None:intergenic 30.0%
AATGCAAAGGGTAGAGTATA+TGG - contig223end:17570-17589 MsG0080048152.01.T01:CDS 35.0%
ACACTAATGATTGAAGCTGT+TGG - contig223end:17540-17559 MsG0080048152.01.T01:CDS 35.0%
ATGCAAAGGGTAGAGTATAT+GGG - contig223end:17571-17590 MsG0080048152.01.T01:CDS 35.0%
!!! TTTGTTTTGACGAGCCATAA+TGG + contig223end:17745-17764 None:intergenic 35.0%
CTAATGATTGAAGCTGTTGG+AGG - contig223end:17543-17562 MsG0080048152.01.T01:CDS 40.0%
GTTGGAGGAAGAAATGCAAA+GGG - contig223end:17558-17577 MsG0080048152.01.T01:CDS 40.0%
TGTTGGAGGAAGAAATGCAA+AGG - contig223end:17557-17576 MsG0080048152.01.T01:CDS 40.0%
! TAGAGTATATGGGCTTGGTT+TGG - contig223end:17581-17600 MsG0080048152.01.T01:CDS 40.0%
!! TCTGTTGATAATGCTACTCG+TGG - contig223end:17834-17853 MsG0080048152.01.T01:CDS 40.0%
AAGGGTAGAGTATATGGGCT+TGG - contig223end:17576-17595 MsG0080048152.01.T01:CDS 45.0%
CGTGGAAGCAATGCAAATGT+TGG - contig223end:17852-17871 MsG0080048152.01.T01:CDS 45.0%
GATATTGCTCGTGGTATGAG+TGG - contig223end:17663-17682 MsG0080048152.01.T01:CDS 45.0%
TGGATTTAGAGGTTGAGCCA+CGG + contig223end:17623-17642 None:intergenic 45.0%
!! GTATATGGGCTTGGTTTGGA+TGG - contig223end:17585-17604 MsG0080048152.01.T01:CDS 45.0%
!! TGCAAATGTTGGTGCTGATG+TGG - contig223end:17863-17882 MsG0080048152.01.T01:CDS 45.0%
GGATTTAGAGGTTGAGCCAC+GGG + contig223end:17622-17641 None:intergenic 50.0%
TGGTATGAGTGGAGAGGTTG+AGG - contig223end:17674-17693 MsG0080048152.01.T01:CDS 50.0%
TGCTCGTGGTATGAGTGGAG+AGG - contig223end:17668-17687 MsG0080048152.01.T01:CDS 55.0%
GAGAGGTTGAGGCGCGATCA+AGG - contig223end:17685-17704 MsG0080048152.01.T01:CDS 60.0%
GATGGCAGCATGCGCACCCG+TGG - contig223end:17603-17622 MsG0080048152.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
contig223end gene 17504 17893 17504 ID=MsG0080048152.01;Name=MsG0080048152.01
contig223end mRNA 17504 17893 17504 ID=MsG0080048152.01.T01;Parent=MsG0080048152.01;Name=MsG0080048152.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|129
contig223end exon 17504 17893 17504 ID=MsG0080048152.01.T01:exon:39470;Parent=MsG0080048152.01.T01
contig223end CDS 17504 17893 17504 ID=MsG0080048152.01.T01:cds;Parent=MsG0080048152.01.T01
Gene Sequence

>MsG0080048152.01.T01

ATGAGTGGTACTCAACCCAAGCCTATTGATTATGATACACTAATGATTGAAGCTGTTGGAGGAAGAAATGCAAAGGGTAGAGTATATGGGCTTGGTTTGGATGGCAGCATGCGCACCCGTGGCTCAACCTCTAAATCCAATAAATATTCAAATTATACTGATATTGCTCGTGGTATGAGTGGAGAGGTTGAGGCGCGATCAAGGCAAAAAATTGCAGAGCAACAACAAACAATTAATTCCATTATGGCTCGTCAAAACAAAATGATTGCTGCATTTTCTAAACATGCAAATATAGATTTGGATTCAATGGATTTAGATGAAGATGATCACTCTGTTGATAATGCTACTCGTGGAAGCAATGCAAATGTTGGTGCTGATGTGGAAGAATAA

Protein sequence

>MsG0080048152.01.T01

MSGTQPKPIDYDTLMIEAVGGRNAKGRVYGLGLDGSMRTRGSTSKSNKYSNYTDIARGMSGEVEARSRQKIAEQQQTINSIMARQNKMIAAFSKHANIDLDSMDLDEDDHSVDNATRGSNANVGADVEE*