Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029363.01.T01 | XP_003616845.1 | 87.327 | 434 | 55 | 0 | 1 | 434 | 1 | 434 | 0 | 793 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029363.01.T01 | A0A2P1GIW7 | 33.871 | 434 | 244 | 12 | 2 | 418 | 4 | 411 | 8.68E-76 | 245 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029363.01.T01 | G7K7H3 | 87.327 | 434 | 55 | 0 | 1 | 434 | 1 | 434 | 0.0 | 793 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029363.01.T01 | MTR_5g084870 | 87.327 | 434 | 55 | 0 | 1 | 434 | 1 | 434 | 0.0 | 793 |
| MsG0580029363.01.T01 | MTR_5g084850 | 81.818 | 440 | 73 | 3 | 1 | 437 | 1 | 436 | 0.0 | 734 |
| MsG0580029363.01.T01 | MTR_8g079970 | 76.606 | 436 | 96 | 2 | 1 | 430 | 1 | 436 | 0.0 | 692 |
| MsG0580029363.01.T01 | MTR_8g079950 | 73.991 | 446 | 110 | 3 | 1 | 440 | 1 | 446 | 0.0 | 679 |
| MsG0580029363.01.T01 | MTR_5g084900 | 79.740 | 385 | 51 | 2 | 56 | 440 | 6 | 363 | 0.0 | 620 |
| MsG0580029363.01.T01 | MTR_8g079980 | 57.534 | 438 | 173 | 5 | 1 | 429 | 1 | 434 | 1.95e-176 | 501 |
| MsG0580029363.01.T01 | MTR_3g086020 | 37.150 | 428 | 243 | 10 | 1 | 421 | 3 | 411 | 1.78e-88 | 275 |
| MsG0580029363.01.T01 | MTR_2g007880 | 31.798 | 456 | 268 | 11 | 1 | 429 | 15 | 454 | 2.03e-68 | 224 |
| MsG0580029363.01.T01 | MTR_8g467580 | 31.057 | 454 | 274 | 12 | 1 | 429 | 1 | 440 | 1.05e-54 | 188 |
| MsG0580029363.01.T01 | MTR_8g467590 | 30.586 | 461 | 280 | 15 | 1 | 435 | 1 | 447 | 1.53e-53 | 185 |
| MsG0580029363.01.T01 | MTR_1g094790 | 31.522 | 460 | 259 | 17 | 1 | 424 | 1 | 440 | 8.54e-53 | 183 |
| MsG0580029363.01.T01 | MTR_7g110540 | 64.228 | 123 | 26 | 1 | 1 | 123 | 1 | 105 | 1.39e-44 | 156 |
| MsG0580029363.01.T01 | MTR_7g110540 | 45.395 | 152 | 49 | 3 | 229 | 380 | 93 | 210 | 8.56e-37 | 135 |
| MsG0580029363.01.T01 | MTR_7g085990 | 25.482 | 467 | 282 | 17 | 3 | 418 | 2 | 453 | 7.60e-25 | 106 |
| MsG0580029363.01.T01 | MTR_5g084920 | 84.483 | 58 | 8 | 1 | 1 | 58 | 1 | 57 | 5.85e-24 | 94.7 |
| MsG0580029363.01.T01 | MTR_4g127650 | 23.385 | 449 | 307 | 12 | 2 | 418 | 9 | 452 | 6.36e-23 | 101 |
| MsG0580029363.01.T01 | MTR_3g026950 | 24.270 | 445 | 270 | 17 | 3 | 399 | 2 | 427 | 7.27e-23 | 100 |
| MsG0580029363.01.T01 | MTR_1g040215 | 23.691 | 401 | 261 | 13 | 2 | 377 | 8 | 388 | 7.32e-23 | 100 |
| MsG0580029363.01.T01 | MTR_7g085930 | 24.625 | 467 | 286 | 16 | 3 | 418 | 6 | 457 | 5.02e-22 | 98.6 |
| MsG0580029363.01.T01 | MTR_5g076700 | 23.656 | 465 | 280 | 20 | 9 | 429 | 16 | 449 | 5.89e-22 | 98.2 |
| MsG0580029363.01.T01 | MTR_8g096530 | 24.178 | 426 | 273 | 17 | 23 | 420 | 25 | 428 | 1.35e-21 | 97.1 |
| MsG0580029363.01.T01 | MTR_4g094842 | 26.147 | 436 | 251 | 20 | 3 | 392 | 4 | 414 | 2.36e-21 | 96.3 |
| MsG0580029363.01.T01 | MTR_2g064495 | 21.286 | 451 | 300 | 15 | 1 | 418 | 1 | 429 | 1.81e-20 | 93.6 |
| MsG0580029363.01.T01 | MTR_2g105330 | 21.286 | 451 | 300 | 15 | 1 | 418 | 1 | 429 | 2.90e-20 | 92.8 |
| MsG0580029363.01.T01 | MTR_4g013050 | 23.272 | 434 | 270 | 15 | 10 | 418 | 11 | 406 | 5.80e-20 | 92.0 |
| MsG0580029363.01.T01 | MTR_5g026320 | 24.356 | 427 | 277 | 14 | 2 | 399 | 17 | 426 | 3.83e-19 | 89.7 |
| MsG0580029363.01.T01 | MTR_3g049150 | 23.753 | 421 | 284 | 12 | 10 | 399 | 9 | 423 | 3.90e-19 | 89.7 |
| MsG0580029363.01.T01 | MTR_8g075610 | 22.444 | 401 | 269 | 17 | 41 | 415 | 30 | 414 | 9.62e-19 | 88.2 |
| MsG0580029363.01.T01 | MTR_4g097090 | 24.612 | 451 | 282 | 20 | 11 | 418 | 17 | 452 | 2.72e-18 | 87.4 |
| MsG0580029363.01.T01 | MTR_7g114560 | 27.841 | 176 | 109 | 5 | 9 | 173 | 16 | 184 | 6.06e-17 | 83.2 |
| MsG0580029363.01.T01 | MTR_1g040315 | 23.529 | 323 | 207 | 13 | 2 | 301 | 8 | 313 | 6.21e-17 | 82.8 |
| MsG0580029363.01.T01 | MTR_4g094835 | 22.727 | 374 | 238 | 13 | 47 | 387 | 52 | 407 | 2.03e-16 | 81.6 |
| MsG0580029363.01.T01 | MTR_7g086000 | 22.822 | 482 | 298 | 18 | 1 | 426 | 2 | 465 | 3.36e-16 | 80.9 |
| MsG0580029363.01.T01 | MTR_1g053315 | 22.831 | 438 | 278 | 17 | 1 | 398 | 16 | 433 | 3.62e-16 | 80.9 |
| MsG0580029363.01.T01 | MTR_0190s0020 | 24.524 | 420 | 266 | 16 | 6 | 397 | 19 | 415 | 4.99e-16 | 80.1 |
| MsG0580029363.01.T01 | MTR_8g041770 | 25.445 | 393 | 250 | 16 | 17 | 387 | 31 | 402 | 1.10e-15 | 79.3 |
| MsG0580029363.01.T01 | MTR_7g014200 | 24.306 | 432 | 272 | 15 | 16 | 399 | 20 | 444 | 1.10e-15 | 79.3 |
| MsG0580029363.01.T01 | MTR_5g090810 | 22.328 | 421 | 264 | 15 | 3 | 377 | 10 | 413 | 1.69e-15 | 79.0 |
| MsG0580029363.01.T01 | MTR_7g084910 | 24.113 | 423 | 248 | 17 | 26 | 413 | 38 | 422 | 2.41e-15 | 78.2 |
| MsG0580029363.01.T01 | MTR_5g090830 | 22.565 | 421 | 263 | 15 | 3 | 377 | 10 | 413 | 3.63e-15 | 77.8 |
| MsG0580029363.01.T01 | MTR_7g014620 | 25.236 | 424 | 256 | 19 | 24 | 405 | 13 | 417 | 6.93e-15 | 76.6 |
| MsG0580029363.01.T01 | MTR_4g055170 | 24.508 | 457 | 280 | 15 | 11 | 422 | 20 | 456 | 7.24e-15 | 76.6 |
| MsG0580029363.01.T01 | MTR_1g011660 | 29.487 | 156 | 103 | 4 | 22 | 172 | 23 | 176 | 8.19e-15 | 76.6 |
| MsG0580029363.01.T01 | MTR_3g100150 | 28.049 | 164 | 110 | 5 | 10 | 169 | 18 | 177 | 1.10e-14 | 76.3 |
| MsG0580029363.01.T01 | MTR_7g114490 | 28.249 | 177 | 107 | 7 | 9 | 173 | 16 | 184 | 2.22e-14 | 75.5 |
| MsG0580029363.01.T01 | MTR_3g112180 | 21.719 | 442 | 279 | 15 | 10 | 415 | 19 | 429 | 3.67e-14 | 74.7 |
| MsG0580029363.01.T01 | MTR_2g036780 | 27.108 | 166 | 113 | 4 | 10 | 171 | 19 | 180 | 5.10e-14 | 73.9 |
| MsG0580029363.01.T01 | MTR_1g035890 | 27.976 | 168 | 111 | 5 | 10 | 171 | 18 | 181 | 1.05e-13 | 73.2 |
| MsG0580029363.01.T01 | MTR_4g022290 | 24.419 | 430 | 262 | 16 | 2 | 391 | 3 | 409 | 1.66e-13 | 72.4 |
| MsG0580029363.01.T01 | MTR_3g071320 | 22.646 | 446 | 282 | 19 | 11 | 417 | 13 | 434 | 4.54e-13 | 71.2 |
| MsG0580029363.01.T01 | MTR_5g090880 | 23.892 | 406 | 248 | 16 | 53 | 411 | 61 | 452 | 1.52e-11 | 66.6 |
| MsG0580029363.01.T01 | MTR_4g094832 | 22.414 | 348 | 212 | 14 | 82 | 397 | 82 | 403 | 2.62e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029363.01.T01 | AT3G26040 | 33.705 | 448 | 263 | 13 | 1 | 429 | 1 | 433 | 2.17e-79 | 253 |
| MsG0580029363.01.T01 | AT1G24430 | 32.877 | 438 | 274 | 8 | 1 | 429 | 3 | 429 | 6.23e-73 | 236 |
| MsG0580029363.01.T01 | AT4G15390 | 32.895 | 456 | 261 | 16 | 1 | 429 | 6 | 443 | 1.91e-66 | 219 |
| MsG0580029363.01.T01 | AT4G15390 | 32.895 | 456 | 261 | 16 | 1 | 429 | 6 | 443 | 1.91e-66 | 219 |
| MsG0580029363.01.T01 | AT1G24420 | 34.598 | 448 | 251 | 17 | 3 | 428 | 5 | 432 | 1.77e-65 | 216 |
| MsG0580029363.01.T01 | AT1G24420 | 34.598 | 448 | 251 | 17 | 3 | 428 | 5 | 432 | 1.77e-65 | 216 |
| MsG0580029363.01.T01 | AT3G30280 | 32.379 | 454 | 265 | 14 | 1 | 429 | 4 | 440 | 3.45e-64 | 213 |
| MsG0580029363.01.T01 | AT5G47950 | 32.127 | 442 | 265 | 15 | 1 | 429 | 4 | 423 | 3.99e-60 | 202 |
| MsG0580029363.01.T01 | AT5G47980 | 32.456 | 456 | 261 | 18 | 1 | 428 | 3 | 439 | 1.07e-59 | 201 |
| MsG0580029363.01.T01 | AT5G23970 | 31.195 | 452 | 261 | 18 | 1 | 429 | 1 | 425 | 6.04e-58 | 196 |
| MsG0580029363.01.T01 | AT4G15400 | 29.451 | 455 | 266 | 20 | 2 | 428 | 4 | 431 | 2.93e-42 | 155 |
| MsG0580029363.01.T01 | AT5G63560 | 23.908 | 435 | 271 | 16 | 11 | 415 | 17 | 421 | 1.82e-26 | 111 |
| MsG0580029363.01.T01 | AT2G19070 | 23.333 | 450 | 291 | 16 | 10 | 418 | 11 | 447 | 4.02e-26 | 110 |
| MsG0580029363.01.T01 | AT5G41040 | 22.197 | 446 | 297 | 14 | 1 | 418 | 15 | 438 | 1.75e-23 | 102 |
| MsG0580029363.01.T01 | AT5G41040 | 22.197 | 446 | 297 | 14 | 1 | 418 | 31 | 454 | 2.63e-23 | 102 |
| MsG0580029363.01.T01 | AT3G48720 | 23.462 | 439 | 276 | 18 | 9 | 415 | 15 | 425 | 2.70e-23 | 102 |
| MsG0580029363.01.T01 | AT5G48930 | 22.098 | 448 | 297 | 17 | 1 | 416 | 1 | 428 | 2.96e-23 | 102 |
| MsG0580029363.01.T01 | AT3G50270 | 25.490 | 459 | 294 | 18 | 3 | 427 | 6 | 450 | 1.52e-21 | 97.1 |
| MsG0580029363.01.T01 | AT1G31490 | 24.490 | 392 | 247 | 13 | 11 | 375 | 14 | 383 | 3.64e-21 | 95.9 |
| MsG0580029363.01.T01 | AT5G67150 | 26.733 | 404 | 243 | 17 | 56 | 423 | 58 | 444 | 4.34e-21 | 95.5 |
| MsG0580029363.01.T01 | AT1G65450 | 25.339 | 442 | 276 | 19 | 11 | 419 | 26 | 446 | 2.64e-19 | 90.5 |
| MsG0580029363.01.T01 | AT3G50280 | 24.685 | 397 | 257 | 15 | 56 | 422 | 56 | 440 | 1.95e-16 | 81.6 |
| MsG0580029363.01.T01 | AT1G78990 | 24.119 | 369 | 234 | 15 | 38 | 377 | 46 | 397 | 1.65e-15 | 79.0 |
| MsG0580029363.01.T01 | AT1G32910 | 24.929 | 353 | 214 | 14 | 59 | 377 | 65 | 400 | 1.55e-14 | 75.9 |
| MsG0580029363.01.T01 | AT3G50280 | 24.916 | 297 | 194 | 11 | 144 | 422 | 25 | 310 | 2.23e-12 | 68.2 |
| MsG0580029363.01.T01 | AT5G38130 | 24.384 | 365 | 243 | 14 | 38 | 375 | 52 | 410 | 2.67e-12 | 68.9 |
Find 73 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTCTCTCTAAATAATTT+TGG | 0.114685 | 5:+96010669 | None:intergenic |
| CCAATGTATGAAGCTGATTT+TGG | 0.192287 | 5:-96010555 | MsG0580029363.01.T01:CDS |
| AAAACAATGTCGTATTTAAA+AGG | 0.232108 | 5:-96011007 | MsG0580029363.01.T01:CDS |
| ATCATTCATGAAGTTGAAAA+TGG | 0.240127 | 5:+96011166 | None:intergenic |
| TCTCTCTTTCCCGCAATCTC+TGG | 0.255027 | 5:+96011029 | None:intergenic |
| TAAGCATGCCGTTTCAATAA+TGG | 0.255959 | 5:-96010883 | MsG0580029363.01.T01:CDS |
| TTTGGTCTATACTCTTGTTT+TGG | 0.281436 | 5:+96011510 | None:intergenic |
| AAGCATGCCGTTTCAATAAT+GGG | 0.282854 | 5:-96010882 | MsG0580029363.01.T01:CDS |
| CCCGCAATCTCTGGGAAAAT+AGG | 0.303007 | 5:+96011038 | None:intergenic |
| GGAAATGGAATTAGACGATT+TGG | 0.333152 | 5:-96010736 | MsG0580029363.01.T01:CDS |
| TTAAGAGACTTGATCATTGC+AGG | 0.336243 | 5:+96010972 | None:intergenic |
| TCTTTCCATTGTAATTCATC+AGG | 0.347159 | 5:+96011293 | None:intergenic |
| TGAGAAGGAGTTGGTGATGA+AGG | 0.349993 | 5:+96011608 | None:intergenic |
| CCACTACTGGTTGTTCTTCA+AGG | 0.375732 | 5:-96010509 | MsG0580029363.01.T01:CDS |
| CTCTAGCGTTCGGTTGATCA+TGG | 0.380841 | 5:+96011139 | None:intergenic |
| CTCTCTTTCCCGCAATCTCT+GGG | 0.393969 | 5:+96011030 | None:intergenic |
| GAACATGGAAGATAATTATA+TGG | 0.396575 | 5:-96010433 | MsG0580029363.01.T01:CDS |
| ACCTTGATCATTACATTCAA+TGG | 0.407499 | 5:+96011400 | None:intergenic |
| GTTGGCAAATAAGAAGGAAA+TGG | 0.414374 | 5:-96010751 | MsG0580029363.01.T01:CDS |
| TTTCAACTTCATGAATGATT+GGG | 0.414945 | 5:-96011162 | MsG0580029363.01.T01:CDS |
| TGCAATGATCAAGTCTCTTA+AGG | 0.417289 | 5:-96010970 | MsG0580029363.01.T01:CDS |
| TTTCATCTTCCTCTAGCGTT+CGG | 0.420949 | 5:+96011129 | None:intergenic |
| TTTAGATAGAAGTTGAGATA+AGG | 0.430630 | 5:+96011460 | None:intergenic |
| AGTTGGTGATGAAGGTTTGA+TGG | 0.434934 | 5:+96011616 | None:intergenic |
| ATTTGCATTAGTCACAAGAT+TGG | 0.437201 | 5:-96011200 | MsG0580029363.01.T01:CDS |
| ATCTATGTTGGCAAATAAGA+AGG | 0.439495 | 5:-96010757 | MsG0580029363.01.T01:CDS |
| GGATAGAGTTTGAGATGAGA+AGG | 0.443809 | 5:+96011593 | None:intergenic |
| ATTTAACCCCATTATTGAAA+CGG | 0.449711 | 5:+96010875 | None:intergenic |
| TACAATGGAAAGACATGGAT+TGG | 0.454586 | 5:-96011283 | MsG0580029363.01.T01:CDS |
| AAAGTGTAGGGAACATACTT+TGG | 0.455506 | 5:-96010785 | MsG0580029363.01.T01:CDS |
| TGCAAAGCAGTTGATTTGAA+TGG | 0.457272 | 5:+96011244 | None:intergenic |
| TGTTGATCTTCGTAGAAGAA+TGG | 0.471282 | 5:-96010826 | MsG0580029363.01.T01:CDS |
| TTGGTCTGCCAAATAAAAGA+AGG | 0.478251 | 5:-96010717 | MsG0580029363.01.T01:CDS |
| TCATCAACTCTAAAATTGCT+TGG | 0.481800 | 5:+96010363 | None:intergenic |
| TCCTCCGTCAAGTAAACTAA+AGG | 0.489786 | 5:+96011088 | None:intergenic |
| AGTTTGAGATGAGAAGGAGT+TGG | 0.495837 | 5:+96011599 | None:intergenic |
| CCAAAATCAGCTTCATACAT+TGG | 0.497579 | 5:+96010555 | None:intergenic |
| GTAATTCATCAGGGAACAAA+GGG | 0.498617 | 5:+96011303 | None:intergenic |
| CCAACATGGATCACCACTAC+TGG | 0.503905 | 5:-96010522 | MsG0580029363.01.T01:CDS |
| ACCATTGAATGTAATGATCA+AGG | 0.505189 | 5:-96011401 | MsG0580029363.01.T01:CDS |
| CCTCCGTCAAGTAAACTAAA+GGG | 0.505751 | 5:+96011089 | None:intergenic |
| TCACCCTTTAGTTTACTTGA+CGG | 0.513674 | 5:-96011092 | MsG0580029363.01.T01:CDS |
| AAAATAGGCAAATCCCTTTG+TGG | 0.526064 | 5:+96011053 | None:intergenic |
| TGAAAGTAATTCTATTTCCA+TGG | 0.528837 | 5:+96011646 | None:intergenic |
| ATGTTGTCTATGAAAGAAAG+TGG | 0.533212 | 5:+96011572 | None:intergenic |
| GTGTACAAGTTGTAATTGCA+TGG | 0.534164 | 5:-96010911 | MsG0580029363.01.T01:CDS |
| CCATGCTAAACATAGCCGTC+TGG | 0.534376 | 5:+96010850 | None:intergenic |
| CTTTCCATTGTAATTCATCA+GGG | 0.539048 | 5:+96011294 | None:intergenic |
| CGTAACTTGCTTCAAGCACT+CGG | 0.540621 | 5:+96010632 | None:intergenic |
| TGTAATTCATCAGGGAACAA+AGG | 0.542689 | 5:+96011302 | None:intergenic |
| CTGCTTTGCATGTGGAGGAA+TGG | 0.550846 | 5:-96011231 | MsG0580029363.01.T01:CDS |
| CCAGTAGTGGTGATCCATGT+TGG | 0.553371 | 5:+96010522 | None:intergenic |
| GATCAAGTCTCTTAAGGCAA+TGG | 0.558721 | 5:-96010964 | MsG0580029363.01.T01:CDS |
| CATTGAAGCGATGGTGAACA+TGG | 0.566518 | 5:-96010448 | MsG0580029363.01.T01:CDS |
| CAAATCAACTGCTTTGCATG+TGG | 0.578282 | 5:-96011239 | MsG0580029363.01.T01:CDS |
| ATCAACTGCTTTGCATGTGG+AGG | 0.582292 | 5:-96011236 | MsG0580029363.01.T01:CDS |
| CGAGTGCTTGAAGCAAGTTA+CGG | 0.584233 | 5:-96010631 | MsG0580029363.01.T01:CDS |
| CCAGACGGCTATGTTTAGCA+TGG | 0.586236 | 5:-96010850 | MsG0580029363.01.T01:CDS |
| CATGATCAACCGAACGCTAG+AGG | 0.589331 | 5:-96011138 | MsG0580029363.01.T01:CDS |
| TACACGTGTGGGAGAATGCA+TGG | 0.593460 | 5:+96010929 | None:intergenic |
| AAAATTGCTTGGACAAAGAG+AGG | 0.594452 | 5:+96010374 | None:intergenic |
| TGGTCTGCCAAATAAAAGAA+GGG | 0.595013 | 5:-96010716 | MsG0580029363.01.T01:CDS |
| ATGGATACAAGAGATGGAGA+TGG | 0.601584 | 5:-96010471 | MsG0580029363.01.T01:CDS |
| AGCATGCCGTTTCAATAATG+GGG | 0.603164 | 5:-96010881 | MsG0580029363.01.T01:CDS |
| TGTTCCCTGATGAATTACAA+TGG | 0.607542 | 5:-96011298 | MsG0580029363.01.T01:CDS |
| TGGAGATGGCATTGAAGCGA+TGG | 0.610707 | 5:-96010457 | MsG0580029363.01.T01:CDS |
| AATTACAACTTGTACACGTG+TGG | 0.612109 | 5:+96010917 | None:intergenic |
| CCTTGAAGAACAACCAGTAG+TGG | 0.615956 | 5:+96010509 | None:intergenic |
| ATTACAACTTGTACACGTGT+GGG | 0.628428 | 5:+96010918 | None:intergenic |
| AATGGGGTTAAATTTCCAGA+CGG | 0.630873 | 5:-96010865 | MsG0580029363.01.T01:CDS |
| CCCTTTAGTTTACTTGACGG+AGG | 0.639205 | 5:-96011089 | MsG0580029363.01.T01:CDS |
| TTGGATGGGGAAAACCAACA+TGG | 0.640749 | 5:-96010536 | MsG0580029363.01.T01:CDS |
| TGAATTACAATGGAAAGACA+TGG | 0.640890 | 5:-96011288 | MsG0580029363.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGGAATATTATAATTTTGA+TGG | - | Chr5:96011498-96011517 | MsG0580029363.01.T01:CDS | 15.0% |
| !!! | AGTAAACAAATCTTTATTTT+CGG | + | Chr5:96011392-96011411 | None:intergenic | 15.0% |
| !! | AAAACAATGTCGTATTTAAA+AGG | - | Chr5:96010981-96011000 | MsG0580029363.01.T01:CDS | 20.0% |
| !!! | AAATCTTTATTTTCGGAATA+AGG | + | Chr5:96011385-96011404 | None:intergenic | 20.0% |
| !!! | ATTTGTTTACTTTTAGTAGT+TGG | - | Chr5:96011401-96011420 | MsG0580029363.01.T01:CDS | 20.0% |
| !!! | TCTAAATACTATCATTTTGT+TGG | - | Chr5:96010545-96010564 | MsG0580029363.01.T01:CDS | 20.0% |
| !!! | TCTTTCTCTCTAAATAATTT+TGG | + | Chr5:96011322-96011341 | None:intergenic | 20.0% |
| ! | ATCATTCATGAAGTTGAAAA+TGG | + | Chr5:96010825-96010844 | None:intergenic | 25.0% |
| ! | ATTTAACCCCATTATTGAAA+CGG | + | Chr5:96011116-96011135 | None:intergenic | 25.0% |
| ! | GAACATGGAAGATAATTATA+TGG | - | Chr5:96011555-96011574 | MsG0580029363.01.T01:CDS | 25.0% |
| ! | TTTAGATAGAAGTTGAGATA+AGG | + | Chr5:96010531-96010550 | None:intergenic | 25.0% |
| ! | TTTCAACTTCATGAATGATT+GGG | - | Chr5:96010826-96010845 | MsG0580029363.01.T01:CDS | 25.0% |
| !! | TTTTCAACTTCATGAATGAT+TGG | - | Chr5:96010825-96010844 | MsG0580029363.01.T01:CDS | 25.0% |
| !!! | GCTGTGATATTTTTGAATTT+TGG | + | Chr5:96010499-96010518 | None:intergenic | 25.0% |
| ACCATTGAATGTAATGATCA+AGG | - | Chr5:96010587-96010606 | MsG0580029363.01.T01:CDS | 30.0% | |
| ACCTTGATCATTACATTCAA+TGG | + | Chr5:96010591-96010610 | None:intergenic | 30.0% | |
| ATCTATGTTGGCAAATAAGA+AGG | - | Chr5:96011231-96011250 | MsG0580029363.01.T01:CDS | 30.0% | |
| ATGTTGTCTATGAAAGAAAG+TGG | + | Chr5:96010419-96010438 | None:intergenic | 30.0% | |
| ATTTGCATTAGTCACAAGAT+TGG | - | Chr5:96010788-96010807 | MsG0580029363.01.T01:CDS | 30.0% | |
| CTTTCCATTGTAATTCATCA+GGG | + | Chr5:96010697-96010716 | None:intergenic | 30.0% | |
| TCTTTCCATTGTAATTCATC+AGG | + | Chr5:96010698-96010717 | None:intergenic | 30.0% | |
| TGAATTACAATGGAAAGACA+TGG | - | Chr5:96010700-96010719 | MsG0580029363.01.T01:CDS | 30.0% | |
| ! | AGCATCAATTTTTCCACAAA+GGG | - | Chr5:96010922-96010941 | MsG0580029363.01.T01:CDS | 30.0% |
| ! | CAGATAACCCTTCTTTTATT+TGG | + | Chr5:96011282-96011301 | None:intergenic | 30.0% |
| !! | AGGGTTCAACAATTTTTCAA+TGG | + | Chr5:96010669-96010688 | None:intergenic | 30.0% |
| !! | ATTTTGATGGATACAAGAGA+TGG | - | Chr5:96011511-96011530 | MsG0580029363.01.T01:CDS | 30.0% |
| !! | GGGTTCAACAATTTTTCAAT+GGG | + | Chr5:96010668-96010687 | None:intergenic | 30.0% |
| !! | TCATCAACTCTAAAATTGCT+TGG | + | Chr5:96011628-96011647 | None:intergenic | 30.0% |
| !! | TTACTTTTAGTAGTTGGTGT+AGG | - | Chr5:96011407-96011426 | MsG0580029363.01.T01:CDS | 30.0% |
| !! | TTTGGTCTATACTCTTGTTT+TGG | + | Chr5:96010481-96010500 | None:intergenic | 30.0% |
| !!! | ACTTTGGTTTTCATCTATGT+TGG | - | Chr5:96011219-96011238 | MsG0580029363.01.T01:CDS | 30.0% |
| !!! | TGTGTTGTTTTTGGTTACAA+AGG | - | Chr5:96010610-96010629 | MsG0580029363.01.T01:CDS | 30.0% |
| AAAATAGGCAAATCCCTTTG+TGG | + | Chr5:96010938-96010957 | None:intergenic | 35.0% | |
| AAAATTGCTTGGACAAAGAG+AGG | + | Chr5:96011617-96011636 | None:intergenic | 35.0% | |
| AAAGTGTAGGGAACATACTT+TGG | - | Chr5:96011203-96011222 | MsG0580029363.01.T01:CDS | 35.0% | |
| AATGGGGTTAAATTTCCAGA+CGG | - | Chr5:96011123-96011142 | MsG0580029363.01.T01:CDS | 35.0% | |
| AATTACAACTTGTACACGTG+TGG | + | Chr5:96011074-96011093 | None:intergenic | 35.0% | |
| ATTACAACTTGTACACGTGT+GGG | + | Chr5:96011073-96011092 | None:intergenic | 35.0% | |
| CCAAAATCAGCTTCATACAT+TGG | + | Chr5:96011436-96011455 | None:intergenic | 35.0% | |
| GGAAATGGAATTAGACGATT+TGG | - | Chr5:96011252-96011271 | MsG0580029363.01.T01:CDS | 35.0% | |
| GTAATTCATCAGGGAACAAA+GGG | + | Chr5:96010688-96010707 | None:intergenic | 35.0% | |
| GTGTACAAGTTGTAATTGCA+TGG | - | Chr5:96011077-96011096 | MsG0580029363.01.T01:CDS | 35.0% | |
| GTTGGCAAATAAGAAGGAAA+TGG | - | Chr5:96011237-96011256 | MsG0580029363.01.T01:CDS | 35.0% | |
| TACAATGGAAAGACATGGAT+TGG | - | Chr5:96010705-96010724 | MsG0580029363.01.T01:CDS | 35.0% | |
| TGCAATGATCAAGTCTCTTA+AGG | - | Chr5:96011018-96011037 | MsG0580029363.01.T01:CDS | 35.0% | |
| TGGTCTGCCAAATAAAAGAA+GGG | - | Chr5:96011272-96011291 | MsG0580029363.01.T01:CDS | 35.0% | |
| TGTAATTCATCAGGGAACAA+AGG | + | Chr5:96010689-96010708 | None:intergenic | 35.0% | |
| TGTTCCCTGATGAATTACAA+TGG | - | Chr5:96010690-96010709 | MsG0580029363.01.T01:CDS | 35.0% | |
| TGTTGATCTTCGTAGAAGAA+TGG | - | Chr5:96011162-96011181 | MsG0580029363.01.T01:CDS | 35.0% | |
| TTAAGAGACTTGATCATTGC+AGG | + | Chr5:96011019-96011038 | None:intergenic | 35.0% | |
| TTGGTCTGCCAAATAAAAGA+AGG | - | Chr5:96011271-96011290 | MsG0580029363.01.T01:CDS | 35.0% | |
| ! | AAGCATGCCGTTTCAATAAT+GGG | - | Chr5:96011106-96011125 | MsG0580029363.01.T01:CDS | 35.0% |
| ! | CGTTGTCTGAAAAAAGTGTA+GGG | - | Chr5:96011191-96011210 | MsG0580029363.01.T01:CDS | 35.0% |
| ! | GAGCATCAATTTTTCCACAA+AGG | - | Chr5:96010921-96010940 | MsG0580029363.01.T01:CDS | 35.0% |
| ! | TAAGCATGCCGTTTCAATAA+TGG | - | Chr5:96011105-96011124 | MsG0580029363.01.T01:CDS | 35.0% |
| ! | TCACCCTTTAGTTTACTTGA+CGG | - | Chr5:96010896-96010915 | MsG0580029363.01.T01:CDS | 35.0% |
| !! | CCAATGTATGAAGCTGATTT+TGG | - | Chr5:96011433-96011452 | MsG0580029363.01.T01:CDS | 35.0% |
| !! | GTATGAAGCTGATTTTGGAT+GGG | - | Chr5:96011438-96011457 | MsG0580029363.01.T01:CDS | 35.0% |
| !! | TATGAAGCTGATTTTGGATG+GGG | - | Chr5:96011439-96011458 | MsG0580029363.01.T01:CDS | 35.0% |
| !! | TGCAAAGCAGTTGATTTGAA+TGG | + | Chr5:96010747-96010766 | None:intergenic | 35.0% |
| !! | TGTATGAAGCTGATTTTGGA+TGG | - | Chr5:96011437-96011456 | MsG0580029363.01.T01:CDS | 35.0% |
| !!! | CCTACACTTTTTTCAGACAA+CGG | + | Chr5:96011193-96011212 | None:intergenic | 35.0% |
| !!! | TGATCAAGGTGTGTTGTTTT+TGG | - | Chr5:96010601-96010620 | MsG0580029363.01.T01:CDS | 35.0% |
| ATGGATACAAGAGATGGAGA+TGG | - | Chr5:96011517-96011536 | MsG0580029363.01.T01:CDS | 40.0% | |
| CAAATCAACTGCTTTGCATG+TGG | - | Chr5:96010749-96010768 | MsG0580029363.01.T01:CDS | 40.0% | |
| CCTCCGTCAAGTAAACTAAA+GGG | + | Chr5:96010902-96010921 | None:intergenic | 40.0% | |
| GATCAAGTCTCTTAAGGCAA+TGG | - | Chr5:96011024-96011043 | MsG0580029363.01.T01:CDS | 40.0% | |
| TCCTCCGTCAAGTAAACTAA+AGG | + | Chr5:96010903-96010922 | None:intergenic | 40.0% | |
| TTTCATCTTCCTCTAGCGTT+CGG | + | Chr5:96010862-96010881 | None:intergenic | 40.0% | |
| ! | AGCATGCCGTTTCAATAATG+GGG | - | Chr5:96011107-96011126 | MsG0580029363.01.T01:CDS | 40.0% |
| ! | AGTTTGAGATGAGAAGGAGT+TGG | + | Chr5:96010392-96010411 | None:intergenic | 40.0% |
| ! | CCGTTGTCTGAAAAAAGTGT+AGG | - | Chr5:96011190-96011209 | MsG0580029363.01.T01:CDS | 40.0% |
| ! | GGATAGAGTTTGAGATGAGA+AGG | + | Chr5:96010398-96010417 | None:intergenic | 40.0% |
| !! | AGTTGGTGATGAAGGTTTGA+TGG | + | Chr5:96010375-96010394 | None:intergenic | 40.0% |
| !!! | ACACTTTTTTCAGACAACGG+AGG | + | Chr5:96011190-96011209 | None:intergenic | 40.0% |
| ATCAACTGCTTTGCATGTGG+AGG | - | Chr5:96010752-96010771 | MsG0580029363.01.T01:CDS | 45.0% | |
| CATTGAAGCGATGGTGAACA+TGG | - | Chr5:96011540-96011559 | MsG0580029363.01.T01:CDS | 45.0% | |
| CCTTGAAGAACAACCAGTAG+TGG | + | Chr5:96011482-96011501 | None:intergenic | 45.0% | |
| CGAGTGCTTGAAGCAAGTTA+CGG | - | Chr5:96011357-96011376 | MsG0580029363.01.T01:CDS | 45.0% | |
| TTGGATGGGGAAAACCAACA+TGG | - | Chr5:96011452-96011471 | MsG0580029363.01.T01:CDS | 45.0% | |
| ! | CCACTACTGGTTGTTCTTCA+AGG | - | Chr5:96011479-96011498 | MsG0580029363.01.T01:CDS | 45.0% |
| ! | CCCTTTAGTTTACTTGACGG+AGG | - | Chr5:96010899-96010918 | MsG0580029363.01.T01:CDS | 45.0% |
| ! | CCTATTTTCCCAGAGATTGC+GGG | - | Chr5:96010950-96010969 | MsG0580029363.01.T01:CDS | 45.0% |
| ! | GCCTATTTTCCCAGAGATTG+CGG | - | Chr5:96010949-96010968 | MsG0580029363.01.T01:CDS | 45.0% |
| !! | CGTAACTTGCTTCAAGCACT+CGG | + | Chr5:96011359-96011378 | None:intergenic | 45.0% |
| !! | TGAGAAGGAGTTGGTGATGA+AGG | + | Chr5:96010383-96010402 | None:intergenic | 45.0% |
| CATGATCAACCGAACGCTAG+AGG | - | Chr5:96010850-96010869 | MsG0580029363.01.T01:CDS | 50.0% | |
| CCAACATGGATCACCACTAC+TGG | - | Chr5:96011466-96011485 | MsG0580029363.01.T01:CDS | 50.0% | |
| CCAGACGGCTATGTTTAGCA+TGG | - | Chr5:96011138-96011157 | MsG0580029363.01.T01:CDS | 50.0% | |
| CCAGTAGTGGTGATCCATGT+TGG | + | Chr5:96011469-96011488 | None:intergenic | 50.0% | |
| CCATGCTAAACATAGCCGTC+TGG | + | Chr5:96011141-96011160 | None:intergenic | 50.0% | |
| CTCTAGCGTTCGGTTGATCA+TGG | + | Chr5:96010852-96010871 | None:intergenic | 50.0% | |
| CTCTCTTTCCCGCAATCTCT+GGG | + | Chr5:96010961-96010980 | None:intergenic | 50.0% | |
| CTGCTTTGCATGTGGAGGAA+TGG | - | Chr5:96010757-96010776 | MsG0580029363.01.T01:CDS | 50.0% | |
| TACACGTGTGGGAGAATGCA+TGG | + | Chr5:96011062-96011081 | None:intergenic | 50.0% | |
| TCTCTCTTTCCCGCAATCTC+TGG | + | Chr5:96010962-96010981 | None:intergenic | 50.0% | |
| ! | CCCGCAATCTCTGGGAAAAT+AGG | + | Chr5:96010953-96010972 | None:intergenic | 50.0% |
| ! | TGGAGATGGCATTGAAGCGA+TGG | - | Chr5:96011531-96011550 | MsG0580029363.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 96010344 | 96011666 | 96010344 | ID=MsG0580029363.01;Name=MsG0580029363.01 |
| Chr5 | mRNA | 96010344 | 96011666 | 96010344 | ID=MsG0580029363.01.T01;Parent=MsG0580029363.01;Name=MsG0580029363.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|440 |
| Chr5 | exon | 96010344 | 96011666 | 96010344 | ID=MsG0580029363.01.T01:exon:36100;Parent=MsG0580029363.01.T01 |
| Chr5 | CDS | 96010344 | 96011666 | 96010344 | ID=MsG0580029363.01.T01:cds;Parent=MsG0580029363.01.T01 |
| Gene Sequence |
| Protein sequence |
