AlfalfaGEDB | MsG0580029363.01

M. sativa cultivar ZhongmuNo.1 / MsG0580029363.01

KEGG: -
GO: GO:0016747
PFAM: PF02458

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029363.01.T01	XP_003616845.1	87.327	434	55	0	1	434	1	434	0	793

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029363.01.T01	A0A2P1GIW7	33.871	434	244	12	2	418	4	411	8.68E-76	245

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029363.01.T01	G7K7H3	87.327	434	55	0	1	434	1	434	0.0	793

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0380012536.01	MsG0580029363.01	PPI
MsG0380012544.01	MsG0580029363.01	PPI
MsG0480019042.01	MsG0580029363.01	PPI
MsG0480019043.01	MsG0580029363.01	PPI
MsG0480019045.01	MsG0580029363.01	PPI
MsG0580029361.01	MsG0580029363.01	PPI
MsG0580029363.01	MsG0480019046.01	PPI
MsG0580029363.01	MsG0680034332.01	PPI
MsG0580029363.01	MsG0880043822.01	PPI
MsG0580029363.01	MsG0880043825.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029363.01.T01	MTR_5g084870	87.327	434	55	0	1	434	1	434	0.0	793
MsG0580029363.01.T01	MTR_5g084850	81.818	440	73	3	1	437	1	436	0.0	734
MsG0580029363.01.T01	MTR_8g079970	76.606	436	96	2	1	430	1	436	0.0	692
MsG0580029363.01.T01	MTR_8g079950	73.991	446	110	3	1	440	1	446	0.0	679
MsG0580029363.01.T01	MTR_5g084900	79.740	385	51	2	56	440	6	363	0.0	620
MsG0580029363.01.T01	MTR_8g079980	57.534	438	173	5	1	429	1	434	1.95e-176	501
MsG0580029363.01.T01	MTR_3g086020	37.150	428	243	10	1	421	3	411	1.78e-88	275
MsG0580029363.01.T01	MTR_2g007880	31.798	456	268	11	1	429	15	454	2.03e-68	224
MsG0580029363.01.T01	MTR_8g467580	31.057	454	274	12	1	429	1	440	1.05e-54	188
MsG0580029363.01.T01	MTR_8g467590	30.586	461	280	15	1	435	1	447	1.53e-53	185

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029363.01.T01	AT3G26040	33.705	448	263	13	1	429	1	433	2.17e-79	253
MsG0580029363.01.T01	AT1G24430	32.877	438	274	8	1	429	3	429	6.23e-73	236
MsG0580029363.01.T01	AT4G15390	32.895	456	261	16	1	429	6	443	1.91e-66	219
MsG0580029363.01.T01	AT4G15390	32.895	456	261	16	1	429	6	443	1.91e-66	219
MsG0580029363.01.T01	AT1G24420	34.598	448	251	17	3	428	5	432	1.77e-65	216
MsG0580029363.01.T01	AT1G24420	34.598	448	251	17	3	428	5	432	1.77e-65	216
MsG0580029363.01.T01	AT3G30280	32.379	454	265	14	1	429	4	440	3.45e-64	213
MsG0580029363.01.T01	AT5G47950	32.127	442	265	15	1	429	4	423	3.99e-60	202
MsG0580029363.01.T01	AT5G47980	32.456	456	261	18	1	428	3	439	1.07e-59	201
MsG0580029363.01.T01	AT5G23970	31.195	452	261	18	1	429	1	425	6.04e-58	196

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Find 73 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAATGTCGTATTTAAA+AGG	0.232108	5:-96011007	MsG0580029363.01.T01:CDS
AAAATAGGCAAATCCCTTTG+TGG	0.526064	5:+96011053	None:intergenic
AAAATTGCTTGGACAAAGAG+AGG	0.594452	5:+96010374	None:intergenic
AAAGTGTAGGGAACATACTT+TGG	0.455506	5:-96010785	MsG0580029363.01.T01:CDS
AAGCATGCCGTTTCAATAAT+GGG	0.282854	5:-96010882	MsG0580029363.01.T01:CDS
AATGGGGTTAAATTTCCAGA+CGG	0.630873	5:-96010865	MsG0580029363.01.T01:CDS
AATTACAACTTGTACACGTG+TGG	0.612109	5:+96010917	None:intergenic
ACCATTGAATGTAATGATCA+AGG	0.505189	5:-96011401	MsG0580029363.01.T01:CDS
ACCTTGATCATTACATTCAA+TGG	0.407499	5:+96011400	None:intergenic
AGCATGCCGTTTCAATAATG+GGG	0.603164	5:-96010881	MsG0580029363.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCATTGAATGTAATGATCA+AGG	-	Chr5:96010587-96010606	MsG0580029363.01.T01:CDS	30.0%
ACCTTGATCATTACATTCAA+TGG	+	Chr5:96010591-96010610	None:intergenic	30.0%
ATCTATGTTGGCAAATAAGA+AGG	-	Chr5:96011231-96011250	MsG0580029363.01.T01:CDS	30.0%
ATGTTGTCTATGAAAGAAAG+TGG	+	Chr5:96010419-96010438	None:intergenic	30.0%
ATTTGCATTAGTCACAAGAT+TGG	-	Chr5:96010788-96010807	MsG0580029363.01.T01:CDS	30.0%
CTTTCCATTGTAATTCATCA+GGG	+	Chr5:96010697-96010716	None:intergenic	30.0%
TCTTTCCATTGTAATTCATC+AGG	+	Chr5:96010698-96010717	None:intergenic	30.0%
TGAATTACAATGGAAAGACA+TGG	-	Chr5:96010700-96010719	MsG0580029363.01.T01:CDS	30.0%
AAAATAGGCAAATCCCTTTG+TGG	+	Chr5:96010938-96010957	None:intergenic	35.0%
AAAATTGCTTGGACAAAGAG+AGG	+	Chr5:96011617-96011636	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	96010344	96011666	96010344	ID=MsG0580029363.01;Name=MsG0580029363.01
Chr5	mRNA	96010344	96011666	96010344	ID=MsG0580029363.01.T01;Parent=MsG0580029363.01;Name=MsG0580029363.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|440
Chr5	exon	96010344	96011666	96010344	ID=MsG0580029363.01.T01:exon:36100;Parent=MsG0580029363.01.T01
Chr5	CDS	96010344	96011666	96010344	ID=MsG0580029363.01.T01:cds;Parent=MsG0580029363.01.T01

Gene Sequence

>MsG0580029363.01.T01

ATGGAAATAGAATTACTTTCAAGAGAAACCATCAAACCTTCATCACCAACTCCTTCTCATCTCAAACTCTATCCACTTTCTTTCATAGACAACATTGTTTCACGTAACTATGTGTCATTACTTTACTTTTACAACCAAAACAAGAGTATAGACCAAAATTCAAAAATATCACAGCTTCGAAAATCCTTATCTCAACTTCTATCTAAATACTATCATTTTGTTGGTAGAATTAAAGATAAAACTACCATTGAATGTAATGATCAAGGTGTGTTGTTTTTGGTTACAAAGGTAAAAAACAAAATTTTAGAGATTCTTCAAAATCCCATTGAAAAATTGTTGAACCCTTTGTTCCCTGATGAATTACAATGGAAAGACATGGATTGGAGTGCAAGTTTTATAGCCATTCAAATCAACTGCTTTGCATGTGGAGGAATGGCAATAAGTATTTGCATTAGTCACAAGATTGGTGATGCTTCAACCATTTTCAACTTCATGAATGATTGGGCCATGATCAACCGAACGCTAGAGGAAGATGAAAAAGAGTTATCAGTATCACCCTTTAGTTTACTTGACGGAGGAGCATCAATTTTTCCACAAAGGGATTTGCCTATTTTCCCAGAGATTGCGGGAAAGAGAGAAAACAATGTCGTATTTAAAAGGTTTGTTTTTCAACCTGCAATGATCAAGTCTCTTAAGGCAATGGTAACTTCTAGTTCCATGCATTCTCCCACACGTGTACAAGTTGTAATTGCATGGATTTATAAGCATGCCGTTTCAATAATGGGGTTAAATTTCCAGACGGCTATGTTTAGCATGGTTGTTGATCTTCGTAGAAGAATGGTTCCTCCGTTGTCTGAAAAAAGTGTAGGGAACATACTTTGGTTTTCATCTATGTTGGCAAATAAGAAGGAAATGGAATTAGACGATTTGGTCTGCCAAATAAAAGAAGGGTTATCTGAGTGTTGTAACGTTTATCCAAAATTATTTAGAGAGAAAGAGAACAATAATATATCCGAGTGCTTGAAGCAAGTTACGGAGCCTTATTCCGAAAATAAAGATTTGTTTACTTTTAGTAGTTGGTGTAGGTTTCCAATGTATGAAGCTGATTTTGGATGGGGAAAACCAACATGGATCACCACTACTGGTTGTTCTTCAAGGAATATTATAATTTTGATGGATACAAGAGATGGAGATGGCATTGAAGCGATGGTGAACATGGAAGATAATTATATGGCTAAGTTTGAACGTGATTTTGAGCTTCTTGAGTATACCTCTCTTTGTCCAAGCAATTTTAGAGTTGATGATTGTGTGCACTGTTCTTGA

Protein sequence

>MsG0580029363.01.T01

MEIELLSRETIKPSSPTPSHLKLYPLSFIDNIVSRNYVSLLYFYNQNKSIDQNSKISQLRKSLSQLLSKYYHFVGRIKDKTTIECNDQGVLFLVTKVKNKILEILQNPIEKLLNPLFPDELQWKDMDWSASFIAIQINCFACGGMAISICISHKIGDASTIFNFMNDWAMINRTLEEDEKELSVSPFSLLDGGASIFPQRDLPIFPEIAGKRENNVVFKRFVFQPAMIKSLKAMVTSSSMHSPTRVQVVIAWIYKHAVSIMGLNFQTAMFSMVVDLRRRMVPPLSEKSVGNILWFSSMLANKKEMELDDLVCQIKEGLSECCNVYPKLFREKENNNISECLKQVTEPYSENKDLFTFSSWCRFPMYEADFGWGKPTWITTTGCSSRNIIILMDTRDGDGIEAMVNMEDNYMAKFERDFELLEYTSLCPSNFRVDDCVHCS*