AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029361.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029361.01.T01 MTR_5g084870 92.063 441 34 1 1 441 1 440 0.0 847
MsG0580029361.01.T01 MTR_5g084850 84.247 438 61 4 1 435 1 433 0.0 757
MsG0580029361.01.T01 MTR_8g079970 74.658 438 104 3 1 432 1 437 0.0 684
MsG0580029361.01.T01 MTR_8g079950 73.696 441 109 4 1 435 1 440 0.0 679
MsG0580029361.01.T01 MTR_5g084900 77.895 380 56 3 56 435 6 357 0.0 600
MsG0580029361.01.T01 MTR_8g079980 58.087 439 170 6 1 430 1 434 6.17e-176 499
MsG0580029361.01.T01 MTR_3g086020 36.512 430 244 12 1 422 3 411 7.26e-83 261
MsG0580029361.01.T01 MTR_2g007880 33.040 454 264 11 1 429 15 453 5.58e-71 231
MsG0580029361.01.T01 MTR_8g467580 32.159 454 270 14 1 430 1 440 5.09e-59 200
MsG0580029361.01.T01 MTR_8g467590 31.209 455 273 14 1 430 1 440 3.27e-55 190
MsG0580029361.01.T01 MTR_1g094790 28.947 456 285 12 1 429 1 444 1.17e-49 175
MsG0580029361.01.T01 MTR_7g110540 48.515 202 68 4 1 190 1 178 4.38e-48 165
MsG0580029361.01.T01 MTR_7g110540 44.737 152 50 2 230 381 93 210 5.58e-37 136
MsG0580029361.01.T01 MTR_7g085990 25.910 467 281 19 3 419 2 453 2.54e-26 111
MsG0580029361.01.T01 MTR_7g085930 25.373 469 287 17 3 420 6 462 1.40e-25 108
MsG0580029361.01.T01 MTR_5g084920 84.483 58 8 1 1 58 1 57 5.74e-25 97.4
MsG0580029361.01.T01 MTR_4g127650 24.379 443 295 15 10 419 17 452 2.29e-24 105
MsG0580029361.01.T01 MTR_3g026950 23.767 446 272 16 3 400 2 427 4.48e-24 104
MsG0580029361.01.T01 MTR_4g007540 20.813 418 293 15 27 419 25 429 8.58e-24 103
MsG0580029361.01.T01 MTR_8g075610 22.475 396 276 15 41 416 30 414 1.15e-23 102
MsG0580029361.01.T01 MTR_3g049150 24.292 424 283 13 10 401 9 426 7.41e-23 100
MsG0580029361.01.T01 MTR_2g064495 21.397 458 292 17 1 419 1 429 7.41e-23 100
MsG0580029361.01.T01 MTR_2g105330 21.652 448 283 17 11 419 11 429 7.63e-23 100
MsG0580029361.01.T01 MTR_4g013050 24.083 436 265 16 10 419 11 406 2.56e-22 99.0
MsG0580029361.01.T01 MTR_1g040215 21.696 401 270 13 2 378 8 388 2.25e-20 93.6
MsG0580029361.01.T01 MTR_8g096530 23.007 439 289 18 23 434 25 441 8.17e-20 91.7
MsG0580029361.01.T01 MTR_5g076700 23.737 396 250 16 9 375 16 388 1.49e-19 91.3
MsG0580029361.01.T01 MTR_7g084940 24.311 399 242 12 28 392 36 408 2.56e-18 87.4
MsG0580029361.01.T01 MTR_5g026320 23.077 429 281 13 2 400 17 426 3.73e-18 86.7
MsG0580029361.01.T01 MTR_7g086000 22.246 472 304 16 1 422 2 460 3.78e-18 87.0
MsG0580029361.01.T01 MTR_3g071320 22.831 438 279 18 11 411 13 428 1.43e-17 84.7
MsG0580029361.01.T01 MTR_4g055170 24.558 452 287 15 11 423 20 456 1.77e-17 84.7
MsG0580029361.01.T01 MTR_7g084910 23.544 412 250 14 28 407 37 415 3.98e-17 83.6
MsG0580029361.01.T01 MTR_7g114560 29.651 172 103 5 9 169 16 180 5.47e-17 83.2
MsG0580029361.01.T01 MTR_4g094838 23.326 433 272 15 17 422 29 428 8.47e-17 82.4
MsG0580029361.01.T01 MTR_1g053315 24.939 409 245 19 10 378 25 411 2.28e-16 81.3
MsG0580029361.01.T01 MTR_7g014200 24.249 433 272 16 16 400 20 444 2.31e-16 81.3
MsG0580029361.01.T01 MTR_5g090830 22.837 416 269 13 3 378 10 413 5.24e-16 80.5
MsG0580029361.01.T01 MTR_7g056087 23.620 453 281 16 3 415 4 431 1.29e-15 79.0
MsG0580029361.01.T01 MTR_3g098980 20.408 441 303 15 2 416 11 429 1.37e-15 79.0
MsG0580029361.01.T01 MTR_1g040175 21.728 405 251 16 9 378 15 388 2.53e-15 78.2
MsG0580029361.01.T01 MTR_4g094842 23.918 439 258 19 3 393 4 414 6.04e-15 77.0
MsG0580029361.01.T01 MTR_4g094835 23.200 375 236 14 47 388 52 407 1.19e-14 75.9
MsG0580029361.01.T01 MTR_8g041770 24.235 392 257 15 17 388 31 402 1.20e-14 75.9
MsG0580029361.01.T01 MTR_0190s0020 24.814 403 243 18 17 388 30 403 1.34e-14 75.9
MsG0580029361.01.T01 MTR_3g112180 22.045 440 283 17 10 417 19 430 1.60e-14 75.9
MsG0580029361.01.T01 MTR_4g022290 24.533 428 259 16 2 389 3 406 1.83e-14 75.5
MsG0580029361.01.T01 MTR_7g114490 29.070 172 104 6 9 169 16 180 2.18e-14 75.5
MsG0580029361.01.T01 MTR_3g100150 28.402 169 112 6 6 169 13 177 4.61e-14 74.3
MsG0580029361.01.T01 MTR_1g011660 27.907 172 110 5 6 172 14 176 9.72e-14 73.2
MsG0580029361.01.T01 MTR_7g014160 24.697 413 247 18 59 419 69 469 1.49e-13 72.8
MsG0580029361.01.T01 MTR_5g090880 24.397 373 235 14 43 376 49 413 1.50e-13 72.8
MsG0580029361.01.T01 MTR_7g114520 30.058 173 102 7 9 169 17 182 1.80e-13 72.4
MsG0580029361.01.T01 MTR_7g014620 24.766 428 254 21 24 406 13 417 1.91e-13 72.4
MsG0580029361.01.T01 MTR_1g040320 28.977 176 107 6 9 173 15 183 4.63e-13 70.9
MsG0580029361.01.T01 MTR_3g102010 23.261 460 309 19 3 429 7 455 2.55e-12 68.9
MsG0580029361.01.T01 MTR_7g114470 21.205 448 261 18 9 405 17 423 2.63e-12 68.9
MsG0580029361.01.T01 MTR_7g027175 20.238 420 288 12 23 416 34 432 2.83e-12 68.6
MsG0580029361.01.T01 MTR_7g014610 25.191 393 234 12 59 400 68 451 6.78e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029361.01.T01 AT3G26040 34.298 449 260 13 1 430 1 433 1.14e-81 259
MsG0580029361.01.T01 AT1G24430 32.574 439 275 8 1 430 3 429 1.84e-71 232
MsG0580029361.01.T01 AT1G24420 34.081 446 257 16 3 429 5 432 5.05e-67 221
MsG0580029361.01.T01 AT1G24420 34.081 446 257 16 3 429 5 432 5.05e-67 221
MsG0580029361.01.T01 AT4G15390 32.823 457 261 16 1 430 6 443 4.45e-65 216
MsG0580029361.01.T01 AT4G15390 32.823 457 261 16 1 430 6 443 4.45e-65 216
MsG0580029361.01.T01 AT3G30280 31.798 456 266 14 1 430 4 440 5.56e-65 215
MsG0580029361.01.T01 AT5G47950 31.364 440 272 12 1 430 4 423 5.21e-63 210
MsG0580029361.01.T01 AT5G23970 30.684 453 263 17 1 430 1 425 2.46e-60 203
MsG0580029361.01.T01 AT5G47980 32.244 459 259 18 1 429 3 439 9.20e-59 199
MsG0580029361.01.T01 AT4G15400 29.047 451 274 18 2 429 4 431 3.05e-42 155
MsG0580029361.01.T01 AT2G19070 25.821 457 286 17 3 419 4 447 3.56e-27 113
MsG0580029361.01.T01 AT5G63560 24.829 439 263 15 11 416 17 421 4.34e-25 107
MsG0580029361.01.T01 AT5G48930 23.266 447 292 16 1 416 1 427 8.94e-23 100
MsG0580029361.01.T01 AT3G48720 25.167 449 259 19 9 417 15 426 3.54e-22 98.6
MsG0580029361.01.T01 AT5G67150 26.065 399 253 17 56 424 58 444 1.17e-18 88.2
MsG0580029361.01.T01 AT1G31490 22.195 410 271 12 2 386 5 391 1.01e-17 85.5
MsG0580029361.01.T01 AT3G50280 23.831 449 304 17 2 423 3 440 4.00e-17 83.6
MsG0580029361.01.T01 AT1G78990 24.496 347 219 14 59 378 67 397 2.02e-15 78.6
MsG0580029361.01.T01 AT2G40230 21.719 442 285 16 10 417 12 426 6.33e-15 77.0
MsG0580029361.01.T01 AT1G32910 25.000 356 211 16 59 378 65 400 9.52e-14 73.6
MsG0580029361.01.T01 AT4G31910 22.747 466 294 18 2 419 8 455 2.59e-13 72.0
MsG0580029361.01.T01 AT5G38130 23.901 364 243 13 40 376 54 410 8.90e-13 70.5
MsG0580029361.01.T01 AT3G50280 25.000 308 210 11 129 423 11 310 2.56e-12 68.2
MsG0580029361.01.T01 AT3G62160 21.320 394 266 13 6 380 8 376 5.15e-12 67.8
MsG0580029361.01.T01 AT1G03390 30.058 173 103 9 11 169 25 193 2.41e-11 65.9

Find 77 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCTCTCTAAATAATTT+TGG 0.114685 5:+95971226 None:intergenic
TCTCTCTTTAACACCATTTC+TGG 0.146770 5:+95971586 None:intergenic
CCAATGTATGAAGCAGATTT+TGG 0.187042 5:-95971112 MsG0580029361.01.T01:CDS
TTAAGAGACTTAATCATTTC+AGG 0.187930 5:+95971529 None:intergenic
AAAACAATGTGGAGTTTAAA+AGG 0.191415 5:-95971564 MsG0580029361.01.T01:CDS
TTTCATCTTCCTCAAACTTT+TGG 0.201806 5:+95971689 None:intergenic
CAAGCATGCCATTTCAATAA+TGG 0.206433 5:-95971440 MsG0580029361.01.T01:CDS
GCATCATCAAGTAAACTTAA+TGG 0.245029 5:+95971649 None:intergenic
TTATAGCCATTCAAATAAAT+TGG 0.252969 5:-95971810 MsG0580029361.01.T01:CDS
GTCATTCATGAAGTTGAAAA+TGG 0.266468 5:+95971726 None:intergenic
AAAGTGTAGGGAACATATTT+TGG 0.268078 5:-95971342 MsG0580029361.01.T01:CDS
AAGCATGCCATTTCAATAAT+GGG 0.288948 5:-95971439 MsG0580029361.01.T01:CDS
ATTTGCATTAGTCACAAAAT+TGG 0.290487 5:-95971760 MsG0580029361.01.T01:CDS
GGAAATGGAATTAGACGATT+TGG 0.333152 5:-95971293 MsG0580029361.01.T01:CDS
TTTGGTCTATACTCTTGTTT+GGG 0.336307 5:+95972070 None:intergenic
TCTTTCCATTGTAATTCATC+AGG 0.347159 5:+95971853 None:intergenic
ATTTAACCCCATTATTGAAA+TGG 0.349368 5:+95971432 None:intergenic
CCACTACTGGTTGTTCTTCA+AGG 0.358918 5:-95971066 MsG0580029361.01.T01:CDS
ACATCATGTCTAGCATTGTT+TGG 0.368697 5:+95970920 None:intergenic
AACACCATTTCTGGGAAGAT+AGG 0.377854 5:+95971595 None:intergenic
TGTAAACCAATTTATTTGAA+TGG 0.388124 5:+95971804 None:intergenic
GAACATGGAAGATAATTATA+TGG 0.400217 5:-95970990 MsG0580029361.01.T01:CDS
CCCTTGGTCATTACATTCAA+TGG 0.400558 5:+95971960 None:intergenic
TGAATTACAATGGAAAGAAA+TGG 0.409552 5:-95971848 MsG0580029361.01.T01:CDS
TGAAATGATTAAGTCTCTTA+AGG 0.413314 5:-95971527 MsG0580029361.01.T01:CDS
GTTGGCAAATAAGAAGGAAA+TGG 0.414374 5:-95971308 MsG0580029361.01.T01:CDS
AGTTGGTGATGAAGGTTTGA+TGG 0.425410 5:+95972176 None:intergenic
CTCAAAGTCATGTTCAAACT+TGG 0.438964 5:+95970967 None:intergenic
CTCTCTTTAACACCATTTCT+GGG 0.439266 5:+95971587 None:intergenic
TTTGCCTATCTTCCCAGAAA+TGG 0.440943 5:-95971599 MsG0580029361.01.T01:CDS
TACAATGGAAAGAAATGGAT+TGG 0.442482 5:-95971843 MsG0580029361.01.T01:CDS
CCAAAATCTGCTTCATACAT+TGG 0.446068 5:+95971112 None:intergenic
GTCTATGTTGGCAAATAAGA+AGG 0.447511 5:-95971314 MsG0580029361.01.T01:CDS
AAACAATGTGGAGTTTAAAA+GGG 0.448288 5:-95971563 MsG0580029361.01.T01:CDS
TTAAGTTTACTTGATGATGC+AGG 0.449285 5:-95971646 MsG0580029361.01.T01:CDS
TGAGATGGAGTTGGTGATGA+AGG 0.467793 5:+95972168 None:intergenic
TTGGTCTGCCAAATAAAAGA+AGG 0.478251 5:-95971274 MsG0580029361.01.T01:CDS
GTAATTCATCAGGGAACAAA+GGG 0.482195 5:+95971863 None:intergenic
CCAACATGGATTACCACTAC+TGG 0.483876 5:-95971079 MsG0580029361.01.T01:CDS
AGCATTGTTTGGATTAAAAG+AGG 0.485125 5:+95970931 None:intergenic
GGGTAAAGTTTGAGGTGAGA+TGG 0.500549 5:+95972153 None:intergenic
TGTCAATCTTCGTAGAAGAA+TGG 0.502319 5:-95971383 MsG0580029361.01.T01:CDS
CATTATAAACCAAAAGTTTG+AGG 0.504187 5:-95971698 MsG0580029361.01.T01:CDS
TTGGTTTACATGTGGAGGAA+TGG 0.504949 5:-95971791 MsG0580029361.01.T01:CDS
CCATGTTAAACATTGCCGTC+TGG 0.505769 5:+95971407 None:intergenic
TGGATACAAGAGATGGAGAT+GGG 0.510515 5:-95971027 MsG0580029361.01.T01:CDS
ATGTTGTCTATGAAAGAAAG+TGG 0.513464 5:+95972132 None:intergenic
AAGAAAGTGGGTAAAGTTTG+AGG 0.514751 5:+95972145 None:intergenic
ATAAATTGGTTTACATGTGG+AGG 0.522702 5:-95971796 MsG0580029361.01.T01:CDS
TAAGTTTACTTGATGATGCA+GGG 0.528419 5:-95971645 MsG0580029361.01.T01:CDS
AAGATAGGCAAATCCCTTTG+TGG 0.529715 5:+95971610 None:intergenic
CTTTCCATTGTAATTCATCA+GGG 0.539048 5:+95971854 None:intergenic
ATGGATACAAGAGATGGAGA+TGG 0.539300 5:-95971028 MsG0580029361.01.T01:CDS
CCATTGAATGTAATGACCAA+GGG 0.539870 5:-95971960 MsG0580029361.01.T01:CDS
CCAGACGGCAATGTTTAACA+TGG 0.540136 5:-95971407 MsG0580029361.01.T01:CDS
TGTAATTCATCAGGGAACAA+AGG 0.542689 5:+95971862 None:intergenic
CAAATAAATTGGTTTACATG+TGG 0.546729 5:-95971799 MsG0580029361.01.T01:CDS
CCAGTAGTGGTAATCCATGT+TGG 0.555600 5:+95971079 None:intergenic
GATTGAAGCGATTGTGAACA+TGG 0.562184 5:-95971005 MsG0580029361.01.T01:CDS
GATTAAGTCTCTTAAGGCAA+TGG 0.565674 5:-95971521 MsG0580029361.01.T01:CDS
TTTCAACTTCATGAATGACT+GGG 0.574249 5:-95971722 MsG0580029361.01.T01:CDS
AGTTACAACTTGTACGCGTG+TGG 0.582993 5:+95971474 None:intergenic
TGGTCTGCCAAATAAAAGAA+GGG 0.595013 5:-95971273 MsG0580029361.01.T01:CDS
TACGCGTGTGGGAGAATGCA+TGG 0.596485 5:+95971486 None:intergenic
AGCATGCCATTTCAATAATG+GGG 0.598350 5:-95971438 MsG0580029361.01.T01:CDS
TGTTGTCTATGAAAGAAAGT+GGG 0.599966 5:+95972133 None:intergenic
ACCATTGAATGTAATGACCA+AGG 0.603495 5:-95971961 MsG0580029361.01.T01:CDS
TGTTCCCTGATGAATTACAA+TGG 0.607542 5:-95971858 MsG0580029361.01.T01:CDS
CCTTGAAGAACAACCAGTAG+TGG 0.609337 5:+95971066 None:intergenic
GCGTACAAGTTGTAACTGCA+TGG 0.613946 5:-95971468 MsG0580029361.01.T01:CDS
TTGGATGGGGAAAACCAACA+TGG 0.618385 5:-95971093 MsG0580029361.01.T01:CDS
AATGGGGTTAAATTTCCAGA+CGG 0.624053 5:-95971422 MsG0580029361.01.T01:CDS
AAGTTTACTTGATGATGCAG+GGG 0.626014 5:-95971644 MsG0580029361.01.T01:CDS
AGTTTGAGGTGAGATGGAGT+TGG 0.644863 5:+95972159 None:intergenic
GTTACAACTTGTACGCGTGT+GGG 0.660957 5:+95971475 None:intergenic
GTTAAAGAGAGAAAACAATG+TGG 0.678799 5:-95971575 MsG0580029361.01.T01:CDS
CATTGAATGTAATGACCAAG+GGG 0.681116 5:-95971959 MsG0580029361.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTATTCTTGGAATA+AGG + Chr5:95971945-95971964 None:intergenic 15.0%
!!! AAGGAATATTATATTTTTGA+TGG - Chr5:95972058-95972077 MsG0580029361.01.T01:CDS 15.0%
!!! AGTAAACAAATTTTTATTCT+TGG + Chr5:95971952-95971971 None:intergenic 15.0%
!! TGTAAACCAATTTATTTGAA+TGG + Chr5:95971304-95971323 None:intergenic 20.0%
!! TTATAGCCATTCAAATAAAT+TGG - Chr5:95971295-95971314 MsG0580029361.01.T01:CDS 20.0%
!!! ATTTGTTTACTTTTAGTAGT+TGG - Chr5:95971961-95971980 MsG0580029361.01.T01:CDS 20.0%
!!! GTTGTGATATTTTTGAATTT+TGG + Chr5:95971056-95971075 None:intergenic 20.0%
!!! TCTTTCTCTCTAAATAATTT+TGG + Chr5:95971882-95971901 None:intergenic 20.0%
!!! TTTTGATAAAACTTGAGATA+AGG + Chr5:95971088-95971107 None:intergenic 20.0%
! AAAACAATGTGGAGTTTAAA+AGG - Chr5:95971541-95971560 MsG0580029361.01.T01:CDS 25.0%
! AAACAATGTGGAGTTTAAAA+GGG - Chr5:95971542-95971561 MsG0580029361.01.T01:CDS 25.0%
! ATTTAACCCCATTATTGAAA+TGG + Chr5:95971676-95971695 None:intergenic 25.0%
! ATTTGCATTAGTCACAAAAT+TGG - Chr5:95971345-95971364 MsG0580029361.01.T01:CDS 25.0%
! CATTATAAACCAAAAGTTTG+AGG - Chr5:95971407-95971426 MsG0580029361.01.T01:CDS 25.0%
! GAACATGGAAGATAATTATA+TGG - Chr5:95972115-95972134 MsG0580029361.01.T01:CDS 25.0%
! TGAAATGATTAAGTCTCTTA+AGG - Chr5:95971578-95971597 MsG0580029361.01.T01:CDS 25.0%
! TGAATTACAATGGAAAGAAA+TGG - Chr5:95971257-95971276 MsG0580029361.01.T01:CDS 25.0%
! TTAAGAGACTTAATCATTTC+AGG + Chr5:95971579-95971598 None:intergenic 25.0%
!! AAACTTGAGATAAGGATTTT+CGG + Chr5:95971080-95971099 None:intergenic 25.0%
!! CAAATAAATTGGTTTACATG+TGG - Chr5:95971306-95971325 MsG0580029361.01.T01:CDS 25.0%
!! CATATAACCCTTCTTTTATT+TGG + Chr5:95971842-95971861 None:intergenic 25.0%
!! TCAAAATACTATCATTTTGC+TGG - Chr5:95971102-95971121 MsG0580029361.01.T01:CDS 25.0%
!!! CTCAAACTTTTGGTTTATAA+TGG + Chr5:95971409-95971428 None:intergenic 25.0%
AGCATTGTTTGGATTAAAAG+AGG + Chr5:95972177-95972196 None:intergenic 30.0%
ATGTTGTCTATGAAAGAAAG+TGG + Chr5:95970976-95970995 None:intergenic 30.0%
CTTTCCATTGTAATTCATCA+GGG + Chr5:95971254-95971273 None:intergenic 30.0%
GCATCATCAAGTAAACTTAA+TGG + Chr5:95971459-95971478 None:intergenic 30.0%
GTCATTCATGAAGTTGAAAA+TGG + Chr5:95971382-95971401 None:intergenic 30.0%
GTTAAAGAGAGAAAACAATG+TGG - Chr5:95971530-95971549 MsG0580029361.01.T01:CDS 30.0%
TACAATGGAAAGAAATGGAT+TGG - Chr5:95971262-95971281 MsG0580029361.01.T01:CDS 30.0%
TCTTTCCATTGTAATTCATC+AGG + Chr5:95971255-95971274 None:intergenic 30.0%
TGTTGTCTATGAAAGAAAGT+GGG + Chr5:95970975-95970994 None:intergenic 30.0%
TTTCAACTTCATGAATGACT+GGG - Chr5:95971383-95971402 MsG0580029361.01.T01:CDS 30.0%
! AAAGTGTAGGGAACATATTT+TGG - Chr5:95971763-95971782 MsG0580029361.01.T01:CDS 30.0%
! AAGCATGCCATTTCAATAAT+GGG - Chr5:95971666-95971685 MsG0580029361.01.T01:CDS 30.0%
! AATACTATCATTTTGCTGGT+AGG - Chr5:95971106-95971125 MsG0580029361.01.T01:CDS 30.0%
! ACAATTTTTCTGTGGGATTT+TGG + Chr5:95971218-95971237 None:intergenic 30.0%
! AGGGTTTAACAATTTTTCTG+TGG + Chr5:95971226-95971245 None:intergenic 30.0%
! ATAAATTGGTTTACATGTGG+AGG - Chr5:95971309-95971328 MsG0580029361.01.T01:CDS 30.0%
! CATTGTCTGAAAAAAGTGTA+GGG - Chr5:95971751-95971770 MsG0580029361.01.T01:CDS 30.0%
! GGGTTTAACAATTTTTCTGT+GGG + Chr5:95971225-95971244 None:intergenic 30.0%
! TTTCATCTTCCTCAAACTTT+TGG + Chr5:95971419-95971438 None:intergenic 30.0%
! TTTGGTCTATACTCTTGTTT+GGG + Chr5:95971038-95971057 None:intergenic 30.0%
! TTTTCAACTTCATGAATGAC+TGG - Chr5:95971382-95971401 MsG0580029361.01.T01:CDS 30.0%
! TTTTGGTCTATACTCTTGTT+TGG + Chr5:95971039-95971058 None:intergenic 30.0%
!! TAAGTTTACTTGATGATGCA+GGG - Chr5:95971460-95971479 MsG0580029361.01.T01:CDS 30.0%
!! TTAAGTTTACTTGATGATGC+AGG - Chr5:95971459-95971478 MsG0580029361.01.T01:CDS 30.0%
!! TTACTTTTAGTAGTTGGTGT+AGG - Chr5:95971967-95971986 MsG0580029361.01.T01:CDS 30.0%
!! TTTTTGATGGATACAAGAGA+TGG - Chr5:95972071-95972090 MsG0580029361.01.T01:CDS 30.0%
!!! ATTTTGGTTTTCGTCTATGT+TGG - Chr5:95971779-95971798 MsG0580029361.01.T01:CDS 30.0%
AAGAAAGTGGGTAAAGTTTG+AGG + Chr5:95970963-95970982 None:intergenic 35.0%
AATGGGGTTAAATTTCCAGA+CGG - Chr5:95971683-95971702 MsG0580029361.01.T01:CDS 35.0%
ACATCATGTCTAGCATTGTT+TGG + Chr5:95972188-95972207 None:intergenic 35.0%
ACCATTGAATGTAATGACCA+AGG - Chr5:95971144-95971163 MsG0580029361.01.T01:CDS 35.0%
CATTGAATGTAATGACCAAG+GGG - Chr5:95971146-95971165 MsG0580029361.01.T01:CDS 35.0%
CCAAAATCTGCTTCATACAT+TGG + Chr5:95971996-95972015 None:intergenic 35.0%
CCATTGAATGTAATGACCAA+GGG - Chr5:95971145-95971164 MsG0580029361.01.T01:CDS 35.0%
CTCAAAGTCATGTTCAAACT+TGG + Chr5:95972141-95972160 None:intergenic 35.0%
CTCTCTTTAACACCATTTCT+GGG + Chr5:95971521-95971540 None:intergenic 35.0%
GATTAAGTCTCTTAAGGCAA+TGG - Chr5:95971584-95971603 MsG0580029361.01.T01:CDS 35.0%
GGAAATGGAATTAGACGATT+TGG - Chr5:95971812-95971831 MsG0580029361.01.T01:CDS 35.0%
GTAATTCATCAGGGAACAAA+GGG + Chr5:95971245-95971264 None:intergenic 35.0%
GTCTATGTTGGCAAATAAGA+AGG - Chr5:95971791-95971810 MsG0580029361.01.T01:CDS 35.0%
GTTGGCAAATAAGAAGGAAA+TGG - Chr5:95971797-95971816 MsG0580029361.01.T01:CDS 35.0%
TCTCTCTTTAACACCATTTC+TGG + Chr5:95971522-95971541 None:intergenic 35.0%
TGGTCTGCCAAATAAAAGAA+GGG - Chr5:95971832-95971851 MsG0580029361.01.T01:CDS 35.0%
TGTAATTCATCAGGGAACAA+AGG + Chr5:95971246-95971265 None:intergenic 35.0%
TGTCAATCTTCGTAGAAGAA+TGG - Chr5:95971722-95971741 MsG0580029361.01.T01:CDS 35.0%
TGTTCCCTGATGAATTACAA+TGG - Chr5:95971247-95971266 MsG0580029361.01.T01:CDS 35.0%
TTGGTCTGCCAAATAAAAGA+AGG - Chr5:95971831-95971850 MsG0580029361.01.T01:CDS 35.0%
! AGCATGCCATTTCAATAATG+GGG - Chr5:95971667-95971686 MsG0580029361.01.T01:CDS 35.0%
! CAAGCATGCCATTTCAATAA+TGG - Chr5:95971665-95971684 MsG0580029361.01.T01:CDS 35.0%
! CCAATGTATGAAGCAGATTT+TGG - Chr5:95971993-95972012 MsG0580029361.01.T01:CDS 35.0%
! CCATTGTCTGAAAAAAGTGT+AGG - Chr5:95971750-95971769 MsG0580029361.01.T01:CDS 35.0%
! GGCAACAATTTTTCCACAAA+GGG - Chr5:95971482-95971501 MsG0580029361.01.T01:CDS 35.0%
! GTATGAAGCAGATTTTGGAT+GGG - Chr5:95971998-95972017 MsG0580029361.01.T01:CDS 35.0%
! TATGAAGCAGATTTTGGATG+GGG - Chr5:95971999-95972018 MsG0580029361.01.T01:CDS 35.0%
! TGTATGAAGCAGATTTTGGA+TGG - Chr5:95971997-95972016 MsG0580029361.01.T01:CDS 35.0%
!! AAGTTTACTTGATGATGCAG+GGG - Chr5:95971461-95971480 MsG0580029361.01.T01:CDS 35.0%
!!! ACACTTTTTTCAGACAATGG+AGG + Chr5:95971750-95971769 None:intergenic 35.0%
!!! CCTACACTTTTTTCAGACAA+TGG + Chr5:95971753-95971772 None:intergenic 35.0%
AAGATAGGCAAATCCCTTTG+TGG + Chr5:95971498-95971517 None:intergenic 40.0%
ATGGATACAAGAGATGGAGA+TGG - Chr5:95972077-95972096 MsG0580029361.01.T01:CDS 40.0%
CCCTTGGTCATTACATTCAA+TGG + Chr5:95971148-95971167 None:intergenic 40.0%
GATTGAAGCGATTGTGAACA+TGG - Chr5:95972100-95972119 MsG0580029361.01.T01:CDS 40.0%
TGGATACAAGAGATGGAGAT+GGG - Chr5:95972078-95972097 MsG0580029361.01.T01:CDS 40.0%
TTGGTTTACATGTGGAGGAA+TGG - Chr5:95971314-95971333 MsG0580029361.01.T01:CDS 40.0%
TTTGCCTATCTTCCCAGAAA+TGG - Chr5:95971506-95971525 MsG0580029361.01.T01:CDS 40.0%
! AACACCATTTCTGGGAAGAT+AGG + Chr5:95971513-95971532 None:intergenic 40.0%
! GGGCAACAATTTTTCCACAA+AGG - Chr5:95971481-95971500 MsG0580029361.01.T01:CDS 40.0%
!! AGTTGGTGATGAAGGTTTGA+TGG + Chr5:95970932-95970951 None:intergenic 40.0%
AGTTACAACTTGTACGCGTG+TGG + Chr5:95971634-95971653 None:intergenic 45.0%
CCAGACGGCAATGTTTAACA+TGG - Chr5:95971698-95971717 MsG0580029361.01.T01:CDS 45.0%
CCAGTAGTGGTAATCCATGT+TGG + Chr5:95972029-95972048 None:intergenic 45.0%
CCATGTTAAACATTGCCGTC+TGG + Chr5:95971701-95971720 None:intergenic 45.0%
CCTTGAAGAACAACCAGTAG+TGG + Chr5:95972042-95972061 None:intergenic 45.0%
GCGTACAAGTTGTAACTGCA+TGG - Chr5:95971637-95971656 MsG0580029361.01.T01:CDS 45.0%
GTTACAACTTGTACGCGTGT+GGG + Chr5:95971633-95971652 None:intergenic 45.0%
TGACCAAAAAGAACACCCCT+TGG + Chr5:95971164-95971183 None:intergenic 45.0%
TTGGATGGGGAAAACCAACA+TGG - Chr5:95972012-95972031 MsG0580029361.01.T01:CDS 45.0%
! AGTTTGAGGTGAGATGGAGT+TGG + Chr5:95970949-95970968 None:intergenic 45.0%
! CCAACATGGATTACCACTAC+TGG - Chr5:95972026-95972045 MsG0580029361.01.T01:CDS 45.0%
! CCACTACTGGTTGTTCTTCA+AGG - Chr5:95972039-95972058 MsG0580029361.01.T01:CDS 45.0%
! GGGTAAAGTTTGAGGTGAGA+TGG + Chr5:95970955-95970974 None:intergenic 45.0%
!! TGAGATGGAGTTGGTGATGA+AGG + Chr5:95970940-95970959 None:intergenic 45.0%
!!! TGACCAAGGGGTGTTCTTTT+TGG - Chr5:95971158-95971177 MsG0580029361.01.T01:CDS 45.0%
TACGCGTGTGGGAGAATGCA+TGG + Chr5:95971622-95971641 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 95970901 95972226 95970901 ID=MsG0580029361.01;Name=MsG0580029361.01
Chr5 mRNA 95970901 95972226 95970901 ID=MsG0580029361.01.T01;Parent=MsG0580029361.01;Name=MsG0580029361.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|441
Chr5 exon 95970901 95972226 95970901 ID=MsG0580029361.01.T01:exon:36083;Parent=MsG0580029361.01.T01
Chr5 CDS 95970901 95972226 95970901 ID=MsG0580029361.01.T01:cds;Parent=MsG0580029361.01.T01
Gene Sequence

>MsG0580029361.01.T01

ATGGAAATTGAATTCGTTTCAAGAGAAACCATCAAACCTTCATCACCAACTCCATCTCACCTCAAACTTTACCCACTTTCTTTCATAGACAACATTATTTTCAGTAACTATGCGTCATTGCTTTACTTTTACAACCCAAACAAGAGTATAGACCAAAATTCAAAAATATCACAACTCCGAAAATCCTTATCTCAAGTTTTATCAAAATACTATCATTTTGCTGGTAGGATTAAAGATAGAATTACCATTGAATGTAATGACCAAGGGGTGTTCTTTTTGGTCACAAAGATAAAAAACAAACTTTCAGAGATTCTCCAAAATCCCACAGAAAAATTGTTAAACCCTTTGTTCCCTGATGAATTACAATGGAAAGAAATGGATTGGAGTGCAAGTTTTATAGCCATTCAAATAAATTGGTTTACATGTGGAGGAATGGTAATAAGTATTTGCATTAGTCACAAAATTGGTGATGCTGCAACCATTTTCAACTTCATGAATGACTGGGCCATTATAAACCAAAAGTTTGAGGAAGATGAAAAAGATTTATTAGTATCACCATTAAGTTTACTTGATGATGCAGGGGCAACAATTTTTCCACAAAGGGATTTGCCTATCTTCCCAGAAATGGTGTTAAAGAGAGAAAACAATGTGGAGTTTAAAAGGGTTGTTTTTCAACCTGAAATGATTAAGTCTCTTAAGGCAATGGTAACTTCTAGTTCCATGCATTCTCCCACACGCGTACAAGTTGTAACTGCATGGATTTACAAGCATGCCATTTCAATAATGGGGTTAAATTTCCAGACGGCAATGTTTAACATGGTTGTCAATCTTCGTAGAAGAATGGTTCCTCCATTGTCTGAAAAAAGTGTAGGGAACATATTTTGGTTTTCGTCTATGTTGGCAAATAAGAAGGAAATGGAATTAGACGATTTGGTCTGCCAAATAAAAGAAGGGTTATATGAGTGTTGTAACGTTTATCCAAAATTATTTAGAGAGAAAGAGAACAATAATATATCTGAGTGCTTGAAGCAAGTTACACAACCTTATTCCAAGAATAAAAATTTGTTTACTTTTAGTAGTTGGTGTAGGTTTCCAATGTATGAAGCAGATTTTGGATGGGGAAAACCAACATGGATTACCACTACTGGTTGTTCTTCAAGGAATATTATATTTTTGATGGATACAAGAGATGGAGATGGGATTGAAGCGATTGTGAACATGGAAGATAATTATATGGCCAAGTTTGAACATGACTTTGAGCTTCTTCAATATGCCTCTTTTAATCCAAACAATGCTAGACATGATGTAATACCCTCAAAATTGTAA
Protein sequence

>MsG0580029361.01.T01

MEIEFVSRETIKPSSPTPSHLKLYPLSFIDNIIFSNYASLLYFYNPNKSIDQNSKISQLRKSLSQVLSKYYHFAGRIKDRITIECNDQGVFFLVTKIKNKLSEILQNPTEKLLNPLFPDELQWKEMDWSASFIAIQINWFTCGGMVISICISHKIGDAATIFNFMNDWAIINQKFEEDEKDLLVSPLSLLDDAGATIFPQRDLPIFPEMVLKRENNVEFKRVVFQPEMIKSLKAMVTSSSMHSPTRVQVVTAWIYKHAISIMGLNFQTAMFNMVVNLRRRMVPPLSEKSVGNIFWFSSMLANKKEMELDDLVCQIKEGLYECCNVYPKLFREKENNNISECLKQVTQPYSKNKNLFTFSSWCRFPMYEADFGWGKPTWITTTGCSSRNIIFLMDTRDGDGIEAIVNMEDNYMAKFEHDFELLQYASFNPNNARHDVIPSKL*