AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019043.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019043.01.T01 MTR_4g022800 83.973 443 70 1 1 442 1 443 0.0 785
MsG0480019043.01.T01 MTR_4g022670 84.163 442 70 0 1 442 1 442 0.0 784
MsG0480019043.01.T01 MTR_4g022720 82.805 442 70 1 1 442 1 436 0.0 766
MsG0480019043.01.T01 MTR_7g026860 80.543 442 83 1 1 442 1 439 0.0 754
MsG0480019043.01.T01 MTR_4g022760 81.222 442 39 1 1 442 1 398 0.0 725
MsG0480019043.01.T01 MTR_4g022825 73.016 441 118 1 1 441 1 440 0.0 663
MsG0480019043.01.T01 MTR_3g088420 67.195 442 137 2 1 442 1 434 0.0 597
MsG0480019043.01.T01 MTR_3g026310 65.924 449 146 2 1 442 1 449 0.0 590
MsG0480019043.01.T01 MTR_0190s0020 57.743 452 170 7 1 441 1 442 0.0 513
MsG0480019043.01.T01 MTR_8g041770 57.048 454 171 7 1 442 1 442 3.16e-174 495
MsG0480019043.01.T01 MTR_4g094842 55.338 459 172 10 1 439 1 446 5.98e-162 464
MsG0480019043.01.T01 MTR_4g094835 52.747 455 184 8 1 437 1 442 8.78e-158 454
MsG0480019043.01.T01 MTR_4g094838 49.891 457 177 9 4 437 1 428 4.78e-143 416
MsG0480019043.01.T01 MTR_4g022290 49.675 461 192 12 1 439 1 443 8.95e-143 415
MsG0480019043.01.T01 MTR_8g079710 50.221 452 187 11 1 431 1 435 1.19e-140 410
MsG0480019043.01.T01 MTR_6g016815 51.131 442 142 7 1 442 15 382 5.01e-140 406
MsG0480019043.01.T01 MTR_4g094828 49.675 461 201 12 1 441 1 450 9.75e-139 405
MsG0480019043.01.T01 MTR_4g094832 55.875 383 142 6 69 437 57 426 7.23e-138 402
MsG0480019043.01.T01 MTR_3g101930 40.583 446 231 11 16 440 22 454 2.68e-94 292
MsG0480019043.01.T01 MTR_3g101900 39.588 437 239 10 18 441 24 448 3.61e-93 288
MsG0480019043.01.T01 MTR_7g026770 69.697 198 32 1 141 338 132 301 2.12e-92 281
MsG0480019043.01.T01 MTR_7g026850 73.410 173 45 1 23 195 7 178 1.43e-89 270
MsG0480019043.01.T01 MTR_3g102020 38.826 443 242 10 16 441 21 451 1.65e-89 280
MsG0480019043.01.T01 MTR_3g101960 38.673 437 240 10 16 436 21 445 3.08e-87 273
MsG0480019043.01.T01 MTR_3g101970 37.955 440 234 10 16 440 21 436 1.83e-85 268
MsG0480019043.01.T01 MTR_8g040760 62.441 213 73 3 1 207 1 212 4.80e-85 259
MsG0480019043.01.T01 MTR_3g102010 37.330 442 249 10 16 441 22 451 5.71e-83 263
MsG0480019043.01.T01 MTR_8g040780 57.407 216 88 2 225 436 12 227 4.11e-77 240
MsG0480019043.01.T01 MTR_3g070170 31.809 481 267 14 2 441 10 470 1.82e-59 202
MsG0480019043.01.T01 MTR_4g015180 32.649 487 251 14 16 442 19 488 2.73e-59 202
MsG0480019043.01.T01 MTR_5g081960 29.752 484 258 12 16 437 19 482 1.20e-56 194
MsG0480019043.01.T01 MTR_4g094825 50.794 189 84 5 17 199 84 269 6.51e-51 177
MsG0480019043.01.T01 MTR_4g094825 51.266 158 64 2 286 441 245 391 7.56e-43 155
MsG0480019043.01.T01 MTR_6g045553 29.916 478 271 13 4 437 14 471 4.63e-50 177
MsG0480019043.01.T01 MTR_3g102030 34.545 330 174 6 73 397 28 320 1.30e-49 171
MsG0480019043.01.T01 MTR_7g026800 50.581 172 54 3 211 380 18 160 3.54e-45 155
MsG0480019043.01.T01 MTR_4g022780 50.000 168 54 2 211 377 19 157 1.28e-44 154
MsG0480019043.01.T01 MTR_6g034485 60.606 132 44 2 189 320 2 125 2.05e-44 151
MsG0480019043.01.T01 MTR_6g034495 58.955 134 47 2 54 179 28 161 2.61e-44 152
MsG0480019043.01.T01 MTR_7g026830 77.011 87 20 0 80 166 12 98 5.84e-41 141
MsG0480019043.01.T01 MTR_7g026780 63.964 111 16 2 332 442 26 112 1.24e-38 135
MsG0480019043.01.T01 MTR_8g075610 26.385 379 260 10 55 428 47 411 1.98e-30 122
MsG0480019043.01.T01 MTR_4g007540 27.820 399 233 14 55 428 56 424 1.76e-28 117
MsG0480019043.01.T01 MTR_2g064495 28.424 387 245 14 55 428 56 423 1.82e-27 114
MsG0480019043.01.T01 MTR_2g105330 28.424 387 245 14 55 428 56 423 2.38e-27 113
MsG0480019043.01.T01 MTR_7g026870 88.889 54 6 0 298 351 98 151 9.01e-27 105
MsG0480019043.01.T01 MTR_8g096530 28.329 413 239 19 49 431 53 438 7.96e-24 103
MsG0480019043.01.T01 MTR_3g026950 26.385 379 225 15 48 393 47 404 4.19e-23 101
MsG0480019043.01.T01 MTR_7g026880 75.000 52 13 0 390 441 2 53 1.58e-22 90.5
MsG0480019043.01.T01 MTR_2g089755 23.908 435 289 13 24 425 37 462 2.84e-21 96.3
MsG0480019043.01.T01 MTR_4g013050 27.273 363 198 13 50 393 48 363 2.99e-21 95.9
MsG0480019043.01.T01 MTR_2g089650 24.940 417 265 17 38 420 43 445 4.86e-21 95.5
MsG0480019043.01.T01 MTR_3g098980 27.198 364 212 16 49 393 60 389 8.44e-21 94.7
MsG0480019043.01.T01 MTR_3g049150 26.075 372 234 15 52 399 52 406 5.91e-20 92.4
MsG0480019043.01.T01 MTR_7g014250 25.882 425 251 17 14 396 11 413 2.12e-19 90.5
MsG0480019043.01.T01 MTR_7g014230 28.505 428 223 19 18 396 22 415 2.26e-19 90.5
MsG0480019043.01.T01 MTR_2g089670 27.105 380 242 14 38 392 60 429 2.28e-19 90.5
MsG0480019043.01.T01 MTR_8g012750 26.304 441 254 16 3 392 2 422 3.68e-19 90.1
MsG0480019043.01.T01 MTR_7g014330 26.202 416 248 16 18 396 20 413 3.68e-19 89.7
MsG0480019043.01.T01 MTR_6g015830 24.947 469 267 16 10 430 21 452 3.51e-18 87.0
MsG0480019043.01.T01 MTR_1g081660 24.505 404 233 17 24 393 31 396 5.16e-18 86.3
MsG0480019043.01.T01 MTR_2g089765 25.472 424 247 16 17 392 32 434 5.99e-18 86.3
MsG0480019043.01.T01 MTR_3g087640 25.424 472 294 14 1 428 5 462 1.41e-17 85.1
MsG0480019043.01.T01 MTR_3g028210 24.731 465 292 16 4 428 8 454 3.08e-17 84.0
MsG0480019043.01.T01 MTR_7g027175 24.936 389 262 13 49 428 62 429 3.57e-17 83.6
MsG0480019043.01.T01 MTR_6g015850 25.333 450 284 16 10 429 21 448 7.13e-17 82.8
MsG0480019043.01.T01 MTR_7g015860 21.918 438 265 15 4 393 5 413 2.55e-16 81.3
MsG0480019043.01.T01 MTR_7g114490 25.508 443 267 20 25 432 24 438 4.97e-16 80.5
MsG0480019043.01.T01 MTR_6g015815 24.503 453 280 17 15 430 25 452 5.46e-16 80.1
MsG0480019043.01.T01 MTR_4g097090 24.808 391 256 13 55 422 65 440 1.59e-15 79.0
MsG0480019043.01.T01 MTR_2g089735 25.236 424 270 17 41 430 43 453 1.66e-15 79.0
MsG0480019043.01.T01 MTR_1g040215 24.828 435 269 19 25 428 23 430 2.08e-15 78.6
MsG0480019043.01.T01 MTR_1g040175 28.520 277 174 12 55 324 64 323 2.13e-15 78.6
MsG0480019043.01.T01 MTR_7g114470 22.936 436 278 16 25 428 25 434 2.63e-15 78.2
MsG0480019043.01.T01 MTR_7g114520 23.529 391 254 14 25 393 25 392 4.12e-15 77.4
MsG0480019043.01.T01 MTR_4g127650 24.623 398 261 12 55 429 66 447 4.56e-15 77.4
MsG0480019043.01.T01 MTR_7g084940 29.433 282 173 12 53 324 67 332 7.16e-15 76.6
MsG0480019043.01.T01 MTR_7g114550 24.771 436 268 17 25 428 24 431 8.38e-15 76.6
MsG0480019043.01.T01 MTR_5g090830 24.870 386 233 13 43 393 49 412 2.19e-14 75.5
MsG0480019043.01.T01 MTR_5g076700 23.624 436 274 17 25 428 24 432 2.87e-14 75.1
MsG0480019043.01.T01 MTR_7g014360 29.286 280 151 10 138 396 90 343 8.10e-14 73.2
MsG0480019043.01.T01 MTR_3g071320 26.332 319 212 9 27 334 37 343 1.29e-13 72.8
MsG0480019043.01.T01 MTR_4g055170 27.213 305 196 10 55 351 69 355 1.76e-13 72.4
MsG0480019043.01.T01 MTR_1g040315 27.389 314 190 13 25 318 23 318 5.65e-13 70.9
MsG0480019043.01.T01 MTR_7g114560 24.771 436 268 19 25 428 24 431 1.67e-12 69.3
MsG0480019043.01.T01 MTR_3g112180 23.575 386 269 12 52 428 58 426 3.52e-12 68.6
MsG0480019043.01.T01 MTR_6g053810 25.694 288 174 12 55 324 71 336 3.48e-11 65.5
MsG0480019043.01.T01 MTR_7g084910 27.208 283 166 13 53 317 68 328 3.82e-11 65.1
MsG0480019043.01.T01 MTR_7g014200 24.009 429 256 17 15 396 19 424 4.57e-11 65.1
MsG0480019043.01.T01 MTR_3g049140 26.042 192 118 6 226 399 18 203 6.39e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019043.01.T01 AT5G67150 39.955 448 242 12 6 437 6 442 5.99e-96 296
MsG0480019043.01.T01 AT5G67160 38.964 444 240 11 6 441 12 432 3.72e-90 280
MsG0480019043.01.T01 AT3G50300 40.138 436 235 13 1 422 1 424 5.17e-90 280
MsG0480019043.01.T01 AT3G50280 37.333 450 260 10 1 438 1 440 1.57e-88 276
MsG0480019043.01.T01 AT5G42830 37.045 440 253 10 15 441 21 449 5.40e-85 268
MsG0480019043.01.T01 AT3G50270 37.802 455 259 12 2 441 3 448 1.94e-83 263
MsG0480019043.01.T01 AT5G07860 37.079 445 243 11 15 440 22 448 1.77e-78 251
MsG0480019043.01.T01 AT5G07850 37.753 445 248 11 15 442 22 454 2.17e-78 251
MsG0480019043.01.T01 AT5G38130 36.468 436 249 12 1 422 21 442 1.58e-75 243
MsG0480019043.01.T01 AT5G07870 36.653 472 249 14 4 442 8 462 5.65e-73 237
MsG0480019043.01.T01 AT2G39980 31.875 480 255 12 16 442 21 481 5.68e-66 219
MsG0480019043.01.T01 AT5G01210 32.979 470 252 12 16 441 19 469 1.43e-61 207
MsG0480019043.01.T01 AT3G50280 36.792 318 185 6 127 438 3 310 8.26e-61 201
MsG0480019043.01.T01 AT3G50290 40.433 277 146 7 122 390 4 269 5.97e-54 181
MsG0480019043.01.T01 AT5G23940 30.464 453 289 13 4 437 3 448 2.85e-53 186
MsG0480019043.01.T01 AT5G48930 29.049 389 245 16 53 428 54 424 5.21e-30 121
MsG0480019043.01.T01 AT3G48720 27.714 350 217 14 55 393 61 385 8.80e-22 97.4
MsG0480019043.01.T01 AT3G29590 24.295 461 291 14 3 431 7 441 1.58e-21 97.1
MsG0480019043.01.T01 AT5G57840 27.865 445 257 20 11 422 9 422 1.39e-20 94.0
MsG0480019043.01.T01 AT5G41040 26.741 359 223 15 49 393 63 395 1.93e-20 93.6
MsG0480019043.01.T01 AT5G41040 26.741 359 223 15 49 393 79 411 1.96e-20 94.0
MsG0480019043.01.T01 AT2G19070 24.263 441 281 16 27 430 19 443 3.72e-20 92.8
MsG0480019043.01.T01 AT3G03480 27.361 413 241 21 25 411 33 412 4.81e-20 92.4
MsG0480019043.01.T01 AT3G29670 22.075 453 309 13 3 430 8 441 8.30e-20 92.0
MsG0480019043.01.T01 AT5G39080 24.459 462 282 22 14 430 14 453 3.17e-17 84.0
MsG0480019043.01.T01 AT5G61160 24.528 371 223 15 52 393 61 403 1.27e-16 82.0
MsG0480019043.01.T01 AT3G29635 24.427 393 258 14 24 392 29 406 1.64e-16 82.0
MsG0480019043.01.T01 AT2G25150 24.500 400 257 16 52 427 64 442 2.78e-16 81.3
MsG0480019043.01.T01 AT5G39090 28.467 274 166 13 135 392 140 399 1.43e-15 79.0
MsG0480019043.01.T01 AT1G28680 24.020 408 247 17 15 395 18 389 1.82e-15 78.6
MsG0480019043.01.T01 AT1G32910 26.741 359 223 16 53 393 63 399 1.60e-14 75.9
MsG0480019043.01.T01 AT1G28680 24.773 331 204 13 15 325 18 323 1.70e-14 75.5
MsG0480019043.01.T01 AT5G16410 25.581 387 236 19 41 402 77 436 3.59e-14 74.7
MsG0480019043.01.T01 AT1G03390 26.184 359 224 18 52 393 73 407 5.70e-14 73.9
MsG0480019043.01.T01 AT5G07080 25.301 332 200 13 14 322 25 331 5.81e-14 73.9
MsG0480019043.01.T01 AT1G78990 25.284 352 232 13 53 393 65 396 1.42e-13 72.8
MsG0480019043.01.T01 AT1G24430 25.240 416 263 18 41 442 45 426 3.53e-13 71.6
MsG0480019043.01.T01 AT5G17540 24.872 390 248 16 25 393 23 388 6.53e-13 70.9
MsG0480019043.01.T01 AT3G30280 23.481 362 229 12 49 393 56 386 8.78e-13 70.5
MsG0480019043.01.T01 AT5G23970 24.161 447 294 18 4 437 3 417 2.72e-12 68.6
MsG0480019043.01.T01 AT5G39050 24.916 297 179 9 149 415 160 442 3.11e-12 68.6

Find 98 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATACGATTTCCTTTCTTTA+TGG 0.108680 4:-13722290 MsG0480019043.01.T01:CDS
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4:-13721767 MsG0480019043.01.T01:CDS
CTGTCTTCCTAACGAGATTT+TGG 0.116738 4:-13721636 MsG0480019043.01.T01:CDS
CCATTACCAGAATATTATTT+TGG 0.150661 4:-13721998 MsG0480019043.01.T01:CDS
AATGAAAGTTGGGGAGTTAT+TGG 0.206917 4:-13721948 MsG0480019043.01.T01:CDS
ACTTGTTTCAACCAACAAAA+AGG 0.207656 4:-13722800 MsG0480019043.01.T01:CDS
TAAGAAATCCAAGCTTTCTT+AGG 0.256333 4:-13721832 MsG0480019043.01.T01:CDS
CTAGTTGACGGTATCTTTAT+TGG 0.271020 4:-13722460 MsG0480019043.01.T01:CDS
AGTGTCTCAGCCGTTGGATT+TGG 0.276668 4:+13722746 None:intergenic
GCATTTCCAAAATAATATTC+TGG 0.329273 4:+13721992 None:intergenic
GATGTTTATGGTAATGATTT+CGG 0.343194 4:-13721755 MsG0480019043.01.T01:CDS
GTGTCTCAGCCGTTGGATTT+GGG 0.349706 4:+13722747 None:intergenic
ACACCTCATGTTGCATTAGT+TGG 0.353377 4:+13721574 None:intergenic
GGACTTGTTAATGTGTTTGC+TGG 0.362735 4:-13721689 MsG0480019043.01.T01:CDS
CCAAAATAATATTCTGGTAA+TGG 0.371844 4:+13721998 None:intergenic
ATTGTTTGATCACTTGATTT+TGG 0.372145 4:+13722853 None:intergenic
GAGGTACGTAGGCCGGTTCC+AGG 0.380874 4:+13722569 None:intergenic
TGTGGCATTTCATCAATTCA+TGG 0.389208 4:-13722399 MsG0480019043.01.T01:CDS
GTATGGTTAAAATGAAAGTT+GGG 0.393088 4:-13721958 MsG0480019043.01.T01:CDS
TTCAACCATGTTGTTGTTGA+CGG 0.393099 4:-13722430 MsG0480019043.01.T01:CDS
TGTATGGTTAAAATGAAAGT+TGG 0.395906 4:-13721959 MsG0480019043.01.T01:CDS
GCCGGCAAGAGGTTGGAAAA+AGG 0.411903 4:+13722705 None:intergenic
GGAAACCAACGTTCGATTGT+TGG 0.414367 4:+13722331 None:intergenic
TATTAAATATTTCTTCTTGT+GGG 0.414469 4:+13722056 None:intergenic
AAATGCAGTTATAAGTTGTA+TGG 0.434670 4:-13721975 MsG0480019043.01.T01:CDS
CTATTAAATATTTCTTCTTG+TGG 0.439740 4:+13722055 None:intergenic
TATGGTAATGATTTCGGATG+GGG 0.444025 4:-13721749 MsG0480019043.01.T01:CDS
TTGGTTATCCTAAGAAAGCT+TGG 0.453190 4:+13721824 None:intergenic
TACTTGTGTTGCTGACATCC+TGG 0.455685 4:-13722587 MsG0480019043.01.T01:CDS
GTGGCATTTCATCAATTCAT+GGG 0.460215 4:-13722398 MsG0480019043.01.T01:CDS
TGGATTTGGGTTTCTACCAC+CGG 0.460917 4:+13722760 None:intergenic
TATGGGACTTTGAATACCAT+TGG 0.474489 4:+13722309 None:intergenic
AAACAATCCATTTGACTCCA+TGG 0.476645 4:-13722837 MsG0480019043.01.T01:CDS
TGAAGAATCACTACGAGTCT+TGG 0.477110 4:-13721856 MsG0480019043.01.T01:CDS
GACACAACATCCACAAATTG+AGG 0.483588 4:+13721596 None:intergenic
GTGGGTCAAATTGTTTGAAA+CGG 0.484207 4:+13722074 None:intergenic
CGTTAGGAAGACAGATTTGA+AGG 0.485586 4:+13721645 None:intergenic
ATATTTAATAGAGTCGTGAT+AGG 0.490052 4:-13722043 MsG0480019043.01.T01:CDS
AATAGAGTCGTGATAGGAGT+TGG 0.491643 4:-13722037 MsG0480019043.01.T01:CDS
CGTAGTGGATATAAAATGAA+TGG 0.496424 4:-13721710 MsG0480019043.01.T01:CDS
TTCCGTTATTTCAAGACGGC+CGG 0.500417 4:+13722687 None:intergenic
ATGGGACTTTGAATACCATT+GGG 0.505613 4:+13722310 None:intergenic
ACCTGCATTGTTGAATATAA+CGG 0.509721 4:+13722639 None:intergenic
GGAGATCCCATGGAGTCAAA+TGG 0.511534 4:+13722830 None:intergenic
TGCCGGCCGTCTTGAAATAA+CGG 0.512312 4:-13722689 MsG0480019043.01.T01:CDS
TCCGTTATATTCAACAATGC+AGG 0.513008 4:-13722640 MsG0480019043.01.T01:CDS
ATTGCCTCCAAAATCTCGTT+AGG 0.513352 4:+13721629 None:intergenic
GGGTCTTCTTTATCACCATC+CGG 0.514663 4:-13722779 MsG0480019043.01.T01:CDS
CATAAAGAAAGGAAATCGTA+TGG 0.514898 4:+13722291 None:intergenic
AGGCACGTCAAAACCATTGT+TGG 0.518095 4:-13722500 MsG0480019043.01.T01:CDS
TTTATGGTAATGATTTCGGA+TGG 0.520125 4:-13721751 MsG0480019043.01.T01:CDS
GGGAGGTGACCCTCAATTTG+TGG 0.520189 4:-13721606 MsG0480019043.01.T01:CDS
TGTTGCTGACATCCTGGAAC+CGG 0.525755 4:-13722581 MsG0480019043.01.T01:CDS
CAAGACGGCCGGCAAGAGGT+TGG 0.533473 4:+13722698 None:intergenic
GGGGAGTTATTGGAGGAAGG+TGG 0.537462 4:-13721938 MsG0480019043.01.T01:CDS
TTAGAAAAGAATCACTCTGA+TGG 0.541983 4:-13722265 MsG0480019043.01.T01:CDS
AAATAATATTCTGGTAATGG+TGG 0.544089 4:+13722001 None:intergenic
GTTGGGGAGTTATTGGAGGA+AGG 0.545044 4:-13721941 MsG0480019043.01.T01:CDS
GAAAGTTGGGGAGTTATTGG+AGG 0.545140 4:-13721945 MsG0480019043.01.T01:CDS
GAGGAAGGTGGACTTTGTAA+AGG 0.546587 4:-13721926 MsG0480019043.01.T01:CDS
ATTTCAAGACGGCCGGCAAG+AGG 0.547420 4:+13722694 None:intergenic
TATCCACTACGAACTGCCAC+AGG 0.548923 4:+13721722 None:intergenic
TAGTCATTAGCAGTTCTCCA+TGG 0.550448 4:-13721781 MsG0480019043.01.T01:CDS
AACAATCCATTTGACTCCAT+GGG 0.553051 4:-13722836 MsG0480019043.01.T01:CDS
ATAGAGAGTGTCTCAGCCGT+TGG 0.554838 4:+13722740 None:intergenic
TTATGGTAATGATTTCGGAT+GGG 0.560500 4:-13721750 MsG0480019043.01.T01:CDS
TAGAAAAGAATCACTCTGAT+GGG 0.561164 4:-13722264 MsG0480019043.01.T01:CDS
TGGTTGAAACAAGTAGATAA+CGG 0.562482 4:+13722809 None:intergenic
GGTAATGGTGGAACTAACCT+TGG 0.563399 4:+13722013 None:intergenic
GTGTTTGCTGGAATAGAAGA+AGG 0.564349 4:-13721677 MsG0480019043.01.T01:CDS
AGTAGATAACGGAGATCCCA+TGG 0.564523 4:+13722820 None:intergenic
TAGATTATTCCACTTTACAA+AGG 0.571697 4:-13722197 MsG0480019043.01.T01:CDS
ACACAACATCCACAAATTGA+GGG 0.576915 4:+13721597 None:intergenic
ACAATTGGAGGTACGTAGGC+CGG 0.578807 4:+13722562 None:intergenic
TGGTGACTTGATTGTATCAG+TGG 0.580783 4:+13722873 None:intergenic
AACCAACGTTCGATTGTTGG+AGG 0.585090 4:+13722334 None:intergenic
CAAGTAACGGAGCTAGTTGA+CGG 0.586570 4:-13722472 MsG0480019043.01.T01:CDS
ATTGTTGGCTGTTCAAGTAA+CGG 0.588367 4:-13722485 MsG0480019043.01.T01:CDS
ATAAAGAAAGGAAATCGTAT+GGG 0.589028 4:+13722292 None:intergenic
TTTGGGTTTCTACCACCGGA+TGG 0.595045 4:+13722764 None:intergenic
AGTGTTCCGTTATTTCAAGA+CGG 0.599596 4:+13722683 None:intergenic
TATGGTTAAAATGAAAGTTG+GGG 0.603920 4:-13721957 MsG0480019043.01.T01:CDS
ATCGAACGTTGGTTTCCCAA+TGG 0.609560 4:-13722325 MsG0480019043.01.T01:CDS
TTGTATCAGTGGAAAGAACT+CGG 0.615591 4:+13722884 None:intergenic
AAGCCTGTGGCAGTTCGTAG+TGG 0.624075 4:-13721725 MsG0480019043.01.T01:CDS
GTTACTTGAACAGCCAACAA+TGG 0.633211 4:+13722487 None:intergenic
ATCAGTGGAAAGAACTCGGA+CGG 0.636215 4:+13722888 None:intergenic
AACCTCCAACAATCGAACGT+TGG 0.637858 4:-13722336 MsG0480019043.01.T01:CDS
TTTCGGATGGGGAAAGCCTG+TGG 0.644016 4:-13721738 MsG0480019043.01.T01:CDS
TGGTAGAAACCCAAATCCAA+CGG 0.650160 4:-13722756 MsG0480019043.01.T01:CDS
CTTGAAATAACGGAACACTC+AGG 0.658273 4:-13722679 MsG0480019043.01.T01:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4:+13721764 None:intergenic
TGAATTGATGAAATGCCACA+AGG 0.668442 4:+13722402 None:intergenic
TCGTGATAGGAGTTGGACCA+AGG 0.676348 4:-13722030 MsG0480019043.01.T01:CDS
TCTTCTTTATCACCATCCGG+TGG 0.677054 4:-13722776 MsG0480019043.01.T01:CDS
TTGTTGACGGAAAATCCTTG+TGG 0.680826 4:-13722417 MsG0480019043.01.T01:CDS
ATCGACAATTGGAGGTACGT+AGG 0.682001 4:+13722558 None:intergenic
AAGTATTATCTGCTACAGCG+TGG 0.709809 4:+13722605 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAATATTTCTTCTTGT+GGG + Chr4:13722431-13722450 None:intergenic 15.0%
!! CTATTAAATATTTCTTCTTG+TGG + Chr4:13722432-13722451 None:intergenic 20.0%
!!! AATGACTAAGAAATTGTTTT+TGG + Chr4:13722691-13722710 None:intergenic 20.0%
! AAATGCAGTTATAAGTTGTA+TGG - Chr4:13722509-13722528 MsG0480019043.01.T01:CDS 25.0%
! ATAAAGAAAGGAAATCGTAT+GGG + Chr4:13722195-13722214 None:intergenic 25.0%
! ATATTTAATAGAGTCGTGAT+AGG - Chr4:13722441-13722460 MsG0480019043.01.T01:CDS 25.0%
! CCAAAATAATATTCTGGTAA+TGG + Chr4:13722489-13722508 None:intergenic 25.0%
! GCATTTCCAAAATAATATTC+TGG + Chr4:13722495-13722514 None:intergenic 25.0%
! GTATGGTTAAAATGAAAGTT+GGG - Chr4:13722526-13722545 MsG0480019043.01.T01:CDS 25.0%
! TAGATTATTCCACTTTACAA+AGG - Chr4:13722287-13722306 MsG0480019043.01.T01:CDS 25.0%
! TATGGTTAAAATGAAAGTTG+GGG - Chr4:13722527-13722546 MsG0480019043.01.T01:CDS 25.0%
! TCAAAAATCAACAAAGAAGA+CGG - Chr4:13722330-13722349 MsG0480019043.01.T01:CDS 25.0%
! TGTATGGTTAAAATGAAAGT+TGG - Chr4:13722525-13722544 MsG0480019043.01.T01:CDS 25.0%
!! ATTGTTTGATCACTTGATTT+TGG + Chr4:13721634-13721653 None:intergenic 25.0%
!! CCATTACCAGAATATTATTT+TGG - Chr4:13722486-13722505 MsG0480019043.01.T01:CDS 25.0%
!! GATGTTTATGGTAATGATTT+CGG - Chr4:13722729-13722748 MsG0480019043.01.T01:CDS 25.0%
!! GTAGTTTTTATCTCCAATAA+AGG + Chr4:13721962-13721981 None:intergenic 25.0%
!! TTTTCTAACTCCATAAAGAA+AGG + Chr4:13722207-13722226 None:intergenic 25.0%
!!! AAATAATATTCTGGTAATGG+TGG + Chr4:13722486-13722505 None:intergenic 25.0%
!!! TTGTAGAGAAGATATTTTGA+TGG + Chr4:13722358-13722377 None:intergenic 25.0%
AAAAATGAATCGACAATTGG+AGG + Chr4:13721937-13721956 None:intergenic 30.0%
ACCTGCATTGTTGAATATAA+CGG + Chr4:13721848-13721867 None:intergenic 30.0%
ACTTGTTTCAACCAACAAAA+AGG - Chr4:13721684-13721703 MsG0480019043.01.T01:CDS 30.0%
ATTGGAGATAAAAACTACGA+AGG - Chr4:13721964-13721983 MsG0480019043.01.T01:CDS 30.0%
CATAAAGAAAGGAAATCGTA+TGG + Chr4:13722196-13722215 None:intergenic 30.0%
CATACGATTTCCTTTCTTTA+TGG - Chr4:13722194-13722213 MsG0480019043.01.T01:CDS 30.0%
CTTGTTTCAACCAACAAAAA+GGG - Chr4:13721685-13721704 MsG0480019043.01.T01:CDS 30.0%
TAAGAAATCCAAGCTTTCTT+AGG - Chr4:13722652-13722671 MsG0480019043.01.T01:CDS 30.0%
TAGAAAAGAATCACTCTGAT+GGG - Chr4:13722220-13722239 MsG0480019043.01.T01:CDS 30.0%
TGGTTGAAACAAGTAGATAA+CGG + Chr4:13721678-13721697 None:intergenic 30.0%
TTACCATAAACATCAAACCA+TGG + Chr4:13722723-13722742 None:intergenic 30.0%
TTAGAAAAGAATCACTCTGA+TGG - Chr4:13722219-13722238 MsG0480019043.01.T01:CDS 30.0%
! ATAAAGAAGACCCTTTTTGT+TGG + Chr4:13721698-13721717 None:intergenic 30.0%
! GTGATTCTTCAACTTTTCAT+CGG + Chr4:13722619-13722638 None:intergenic 30.0%
! TTATGGTAATGATTTCGGAT+GGG - Chr4:13722734-13722753 MsG0480019043.01.T01:CDS 30.0%
! TTTATGGTAATGATTTCGGA+TGG - Chr4:13722733-13722752 MsG0480019043.01.T01:CDS 30.0%
!! CGTAGTGGATATAAAATGAA+TGG - Chr4:13722774-13722793 MsG0480019043.01.T01:CDS 30.0%
!! GATTCATTTTTCGCCTTTAT+TGG - Chr4:13721946-13721965 MsG0480019043.01.T01:CDS 30.0%
!!! GAAATTGTTTTTGGCTATGT+TGG + Chr4:13722682-13722701 None:intergenic 30.0%
AAACAATCCATTTGACTCCA+TGG - Chr4:13721647-13721666 MsG0480019043.01.T01:CDS 35.0%
AACAATCCATTTGACTCCAT+GGG - Chr4:13721648-13721667 MsG0480019043.01.T01:CDS 35.0%
AAGAGGTTGGAAAAAGGAAA+GGG + Chr4:13721776-13721795 None:intergenic 35.0%
AATGAAAGTTGGGGAGTTAT+TGG - Chr4:13722536-13722555 MsG0480019043.01.T01:CDS 35.0%
ACACAACATCCACAAATTGA+GGG + Chr4:13722890-13722909 None:intergenic 35.0%
ATGGGACTTTGAATACCATT+GGG + Chr4:13722177-13722196 None:intergenic 35.0%
CTAGTTGACGGTATCTTTAT+TGG - Chr4:13722024-13722043 MsG0480019043.01.T01:CDS 35.0%
GCGAAAAATGAATCGACAAT+TGG + Chr4:13721940-13721959 None:intergenic 35.0%
GTGGCATTTCATCAATTCAT+GGG - Chr4:13722086-13722105 MsG0480019043.01.T01:CDS 35.0%
GTGGGTCAAATTGTTTGAAA+CGG + Chr4:13722413-13722432 None:intergenic 35.0%
TATGGGACTTTGAATACCAT+TGG + Chr4:13722178-13722197 None:intergenic 35.0%
TCCGTTATATTCAACAATGC+AGG - Chr4:13721844-13721863 MsG0480019043.01.T01:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG - Chr4:13722717-13722736 MsG0480019043.01.T01:CDS 35.0%
TGAATTGATGAAATGCCACA+AGG + Chr4:13722085-13722104 None:intergenic 35.0%
TGTGGCATTTCATCAATTCA+TGG - Chr4:13722085-13722104 MsG0480019043.01.T01:CDS 35.0%
TTGGTTATCCTAAGAAAGCT+TGG + Chr4:13722663-13722682 None:intergenic 35.0%
TTGTATCAGTGGAAAGAACT+CGG + Chr4:13721603-13721622 None:intergenic 35.0%
! AGTGTTCCGTTATTTCAAGA+CGG + Chr4:13721804-13721823 None:intergenic 35.0%
! ATTGTTGGCTGTTCAAGTAA+CGG - Chr4:13721999-13722018 MsG0480019043.01.T01:CDS 35.0%
! ATTTTCCGTCAACAACAACA+TGG + Chr4:13722062-13722081 None:intergenic 35.0%
! CGATTTTCTCCTTTGTAAAG+TGG + Chr4:13722299-13722318 None:intergenic 35.0%
! TATGGTAATGATTTCGGATG+GGG - Chr4:13722735-13722754 MsG0480019043.01.T01:CDS 35.0%
! TTCAACCATGTTGTTGTTGA+CGG - Chr4:13722054-13722073 MsG0480019043.01.T01:CDS 35.0%
AAGTATTATCTGCTACAGCG+TGG + Chr4:13721882-13721901 None:intergenic 40.0%
AATAGAGTCGTGATAGGAGT+TGG - Chr4:13722447-13722466 MsG0480019043.01.T01:CDS 40.0%
ATTGCCTCCAAAATCTCGTT+AGG + Chr4:13722858-13722877 None:intergenic 40.0%
CAAGAGGTTGGAAAAAGGAA+AGG + Chr4:13721777-13721796 None:intergenic 40.0%
CGTTAGGAAGACAGATTTGA+AGG + Chr4:13722842-13722861 None:intergenic 40.0%
GACACAACATCCACAAATTG+AGG + Chr4:13722891-13722910 None:intergenic 40.0%
GTTACTTGAACAGCCAACAA+TGG + Chr4:13722000-13722019 None:intergenic 40.0%
TAGTCATTAGCAGTTCTCCA+TGG - Chr4:13722703-13722722 MsG0480019043.01.T01:CDS 40.0%
TGAAGAATCACTACGAGTCT+TGG - Chr4:13722628-13722647 MsG0480019043.01.T01:CDS 40.0%
TGGTAGAAACCCAAATCCAA+CGG - Chr4:13721728-13721747 MsG0480019043.01.T01:CDS 40.0%
TGGTGACTTGATTGTATCAG+TGG + Chr4:13721614-13721633 None:intergenic 40.0%
TTGTTGACGGAAAATCCTTG+TGG - Chr4:13722067-13722086 MsG0480019043.01.T01:CDS 40.0%
! CTGTCTTCCTAACGAGATTT+TGG - Chr4:13722848-13722867 MsG0480019043.01.T01:CDS 40.0%
! CTTGAAATAACGGAACACTC+AGG - Chr4:13721805-13721824 MsG0480019043.01.T01:CDS 40.0%
! GTGTTTGCTGGAATAGAAGA+AGG - Chr4:13722807-13722826 MsG0480019043.01.T01:CDS 40.0%
! TCTTCCTAACGAGATTTTGG+AGG - Chr4:13722851-13722870 MsG0480019043.01.T01:CDS 40.0%
!! AACGAGATTTTGGAGGCAAT+GGG - Chr4:13722858-13722877 MsG0480019043.01.T01:CDS 40.0%
!! AAGCGCTTTTAACTCACGTT+TGG - Chr4:13722376-13722395 MsG0480019043.01.T01:CDS 40.0%
!! GGACTTGTTAATGTGTTTGC+TGG - Chr4:13722795-13722814 MsG0480019043.01.T01:CDS 40.0%
!! TAACGAGATTTTGGAGGCAA+TGG - Chr4:13722857-13722876 MsG0480019043.01.T01:CDS 40.0%
AACCAACGTTCGATTGTTGG+AGG + Chr4:13722153-13722172 None:intergenic 45.0%
AACCTCCAACAATCGAACGT+TGG - Chr4:13722148-13722167 MsG0480019043.01.T01:CDS 45.0%
AGGCACGTCAAAACCATTGT+TGG - Chr4:13721984-13722003 MsG0480019043.01.T01:CDS 45.0%
ATCAGTGGAAAGAACTCGGA+CGG + Chr4:13721599-13721618 None:intergenic 45.0%
GAAAGTTGGGGAGTTATTGG+AGG - Chr4:13722539-13722558 MsG0480019043.01.T01:CDS 45.0%
GGAAACCAACGTTCGATTGT+TGG + Chr4:13722156-13722175 None:intergenic 45.0%
GGGTCTTCTTTATCACCATC+CGG - Chr4:13721705-13721724 MsG0480019043.01.T01:CDS 45.0%
TCTTCTTTATCACCATCCGG+TGG - Chr4:13721708-13721727 MsG0480019043.01.T01:CDS 45.0%
TTGGAAAAAGGAAAGGGCAG+AGG + Chr4:13721770-13721789 None:intergenic 45.0%
! ATCGACAATTGGAGGTACGT+AGG + Chr4:13721929-13721948 None:intergenic 45.0%
! CAAGTAACGGAGCTAGTTGA+CGG - Chr4:13722012-13722031 MsG0480019043.01.T01:CDS 45.0%
! GAGGAAGGTGGACTTTGTAA+AGG - Chr4:13722558-13722577 MsG0480019043.01.T01:CDS 45.0%
! GGTAATGGTGGAACTAACCT+TGG + Chr4:13722474-13722493 None:intergenic 45.0%
! TACTTGTGTTGCTGACATCC+TGG - Chr4:13721897-13721916 MsG0480019043.01.T01:CDS 45.0%
! TCCTTTTTCCAACCTCTTGC+CGG - Chr4:13721778-13721797 MsG0480019043.01.T01:CDS 45.0%
! TGGATTTGGGTTTCTACCAC+CGG + Chr4:13721727-13721746 None:intergenic 45.0%
! TTCCGTTATTTCAAGACGGC+CGG + Chr4:13721800-13721819 None:intergenic 45.0%
!! AGTAGATAACGGAGATCCCA+TGG + Chr4:13721667-13721686 None:intergenic 45.0%
!! ATCGAACGTTGGTTTCCCAA+TGG - Chr4:13722159-13722178 MsG0480019043.01.T01:CDS 45.0%
AGTGTCTCAGCCGTTGGATT+TGG + Chr4:13721741-13721760 None:intergenic 50.0%
GGAGATCCCATGGAGTCAAA+TGG + Chr4:13721657-13721676 None:intergenic 50.0%
GTGTCTCAGCCGTTGGATTT+GGG + Chr4:13721740-13721759 None:intergenic 50.0%
GTTGGGGAGTTATTGGAGGA+AGG - Chr4:13722543-13722562 MsG0480019043.01.T01:CDS 50.0%
TATCCACTACGAACTGCCAC+AGG + Chr4:13722765-13722784 None:intergenic 50.0%
TCGTGATAGGAGTTGGACCA+AGG - Chr4:13722454-13722473 MsG0480019043.01.T01:CDS 50.0%
TGCCGGCCGTCTTGAAATAA+CGG - Chr4:13721795-13721814 MsG0480019043.01.T01:CDS 50.0%
TGTTGCTGACATCCTGGAAC+CGG - Chr4:13721903-13721922 MsG0480019043.01.T01:CDS 50.0%
! ACAATTGGAGGTACGTAGGC+CGG + Chr4:13721925-13721944 None:intergenic 50.0%
! TTTGGGTTTCTACCACCGGA+TGG + Chr4:13721723-13721742 None:intergenic 50.0%
!! ATAGAGAGTGTCTCAGCCGT+TGG + Chr4:13721747-13721766 None:intergenic 50.0%
!! GAGATTTTGGAGGCAATGGG+AGG - Chr4:13722861-13722880 MsG0480019043.01.T01:CDS 50.0%
AAGCCTGTGGCAGTTCGTAG+TGG - Chr4:13722759-13722778 MsG0480019043.01.T01:CDS 55.0%
ATTTCAAGACGGCCGGCAAG+AGG + Chr4:13721793-13721812 None:intergenic 55.0%
GCCGGCAAGAGGTTGGAAAA+AGG + Chr4:13721782-13721801 None:intergenic 55.0%
GGGAGGTGACCCTCAATTTG+TGG - Chr4:13722878-13722897 MsG0480019043.01.T01:CDS 55.0%
GGGGAGTTATTGGAGGAAGG+TGG - Chr4:13722546-13722565 MsG0480019043.01.T01:CDS 55.0%
TTTCGGATGGGGAAAGCCTG+TGG - Chr4:13722746-13722765 MsG0480019043.01.T01:CDS 55.0%
CAAGACGGCCGGCAAGAGGT+TGG + Chr4:13721789-13721808 None:intergenic 65.0%
! GAGGTACGTAGGCCGGTTCC+AGG + Chr4:13721918-13721937 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 13721589 13722917 13721589 ID=MsG0480019043.01;Name=MsG0480019043.01
Chr4 mRNA 13721589 13722917 13721589 ID=MsG0480019043.01.T01;Parent=MsG0480019043.01;Name=MsG0480019043.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|442
Chr4 exon 13721589 13722917 13721589 ID=MsG0480019043.01.T01:exon:8555;Parent=MsG0480019043.01.T01
Chr4 CDS 13721589 13722917 13721589 ID=MsG0480019043.01.T01:cds;Parent=MsG0480019043.01.T01
Gene Sequence

>MsG0480019043.01.T01

ATGGGTGCCGTCCGAGTTCTTTCCACTGATACAATCAAGTCACCAAAATCAAGTGATCAAACAATCCATTTGACTCCATGGGATCTCCGTTATCTACTTGTTTCAACCAACAAAAAGGGTCTTCTTTATCACCATCCGGTGGTAGAAACCCAAATCCAACGGCTGAGACACTCTCTATCCTCTGCCCTTTCCTTTTTCCAACCTCTTGCCGGCCGTCTTGAAATAACGGAACACTCAGGCAACACTGTCTCGTGTTCCGTTATATTCAACAATGCAGGTGTACTCTTTATCCACGCTGTAGCAGATAATACTTGTGTTGCTGACATCCTGGAACCGGCCTACGTACCTCCAATTGTCGATTCATTTTTCGCCTTTATTGGAGATAAAAACTACGAAGGCACGTCAAAACCATTGTTGGCTGTTCAAGTAACGGAGCTAGTTGACGGTATCTTTATTGGTTGCACATTCAACCATGTTGTTGTTGACGGAAAATCCTTGTGGCATTTCATCAATTCATGGGCTGAAATCTCACGTAATTGTTGTCATCATCAAATATCTAAACCTCCAACAATCGAACGTTGGTTTCCCAATGGTATTCAAAGTCCCATACGATTTCCTTTCTTTATGGAGTTAGAAAAGAATCACTCTGATGGGTTGACTATTTCATCATCTAATGATGAAAAACTTTGTTTATCAAATAGATTATTCCACTTTACAAAGGAGAAAATCGTGCAACTTAAATCAAAAATCAACAAAGAAGACGGTACCATCAAAATATCTTCTCTACAAGCGCTTTTAACTCACGTTTGGTGCTATGTGATCCGTTTCAAACAATTTGACCCACAAGAAGAAATATTTAATAGAGTCGTGATAGGAGTTGGACCAAGGTTAGTTCCACCATTACCAGAATATTATTTTGGAAATGCAGTTATAAGTTGTATGGTTAAAATGAAAGTTGGGGAGTTATTGGAGGAAGGTGGACTTTGTAAAGGTGCTTGTGAGATGAACAAGTTAATTGCTTCACACACCGATGAAAAGTTGAAGAATCACTACGAGTCTTGGTTAAGAAATCCAAGCTTTCTTAGGATAACCAACATAGCCAAAAACAATTTCTTAGTCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTCGGATGGGGAAAGCCTGTGGCAGTTCGTAGTGGATATAAAATGAATGGACTTGTTAATGTGTTTGCTGGAATAGAAGAAGGTAGCATTGACCTTCAAATCTGTCTTCCTAACGAGATTTTGGAGGCAATGGGAGGTGACCCTCAATTTGTGGATGTTGTGTCCAACTAA
Protein sequence

>MsG0480019043.01.T01

MGAVRVLSTDTIKSPKSSDQTIHLTPWDLRYLLVSTNKKGLLYHHPVVETQIQRLRHSLSSALSFFQPLAGRLEITEHSGNTVSCSVIFNNAGVLFIHAVADNTCVADILEPAYVPPIVDSFFAFIGDKNYEGTSKPLLAVQVTELVDGIFIGCTFNHVVVDGKSLWHFINSWAEISRNCCHHQISKPPTIERWFPNGIQSPIRFPFFMELEKNHSDGLTISSSNDEKLCLSNRLFHFTKEKIVQLKSKINKEDGTIKISSLQALLTHVWCYVIRFKQFDPQEEIFNRVVIGVGPRLVPPLPEYYFGNAVISCMVKMKVGELLEEGGLCKGACEMNKLIASHTDEKLKNHYESWLRNPSFLRITNIAKNNFLVISSSPWFDVYGNDFGWGKPVAVRSGYKMNGLVNVFAGIEEGSIDLQICLPNEILEAMGGDPQFVDVVSN*