AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043825.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043825.01.T01 MTR_8g041770 88.352 352 34 2 2 353 98 442 0.0 631
MsG0880043825.01.T01 MTR_0190s0020 69.886 352 100 2 1 352 97 442 9.92e-180 506
MsG0880043825.01.T01 MTR_4g022825 62.147 354 127 4 2 351 89 439 1.68e-158 451
MsG0880043825.01.T01 MTR_3g088420 62.994 354 121 4 2 353 89 434 3.24e-157 448
MsG0880043825.01.T01 MTR_4g022670 61.173 358 131 4 1 353 88 442 3.14e-156 446
MsG0880043825.01.T01 MTR_4g022800 60.724 359 132 4 1 353 88 443 1.57e-154 442
MsG0880043825.01.T01 MTR_4g022720 60.674 356 130 4 1 353 88 436 6.78e-153 437
MsG0880043825.01.T01 MTR_3g026310 59.341 364 133 4 2 353 89 449 6.51e-152 435
MsG0880043825.01.T01 MTR_4g094832 59.773 353 127 5 1 348 84 426 9.57e-150 429
MsG0880043825.01.T01 MTR_7g026860 59.664 357 135 4 1 353 88 439 1.08e-149 429
MsG0880043825.01.T01 MTR_4g094835 58.357 353 132 5 1 348 100 442 2.76e-146 421
MsG0880043825.01.T01 MTR_4g094842 58.120 351 140 5 1 350 102 446 5.43e-141 407
MsG0880043825.01.T01 MTR_4g094838 54.958 353 128 5 1 348 102 428 1.52e-130 380
MsG0880043825.01.T01 MTR_4g022760 53.782 357 115 3 1 353 88 398 6.46e-129 375
MsG0880043825.01.T01 MTR_4g094828 50.000 360 170 5 1 352 93 450 1.88e-117 347
MsG0880043825.01.T01 MTR_4g022290 50.000 360 158 5 1 349 94 442 2.83e-113 337
MsG0880043825.01.T01 MTR_8g079710 51.275 353 153 6 1 343 93 436 5.86e-113 336
MsG0880043825.01.T01 MTR_6g016815 48.315 356 104 7 2 353 103 382 2.25e-100 301
MsG0880043825.01.T01 MTR_8g040780 60.648 216 81 3 135 347 13 227 1.79e-86 261
MsG0880043825.01.T01 MTR_3g101900 40.000 355 198 7 3 352 104 448 8.25e-83 259
MsG0880043825.01.T01 MTR_3g101930 38.936 357 200 7 3 351 108 454 2.22e-80 253
MsG0880043825.01.T01 MTR_3g102020 38.592 355 204 5 3 352 106 451 7.16e-78 246
MsG0880043825.01.T01 MTR_3g102010 38.611 360 201 7 3 352 102 451 4.00e-74 236
MsG0880043825.01.T01 MTR_3g101960 37.714 350 204 5 3 347 105 445 7.33e-73 233
MsG0880043825.01.T01 MTR_3g101970 37.429 350 199 5 3 351 106 436 7.83e-73 233
MsG0880043825.01.T01 MTR_3g070170 32.383 386 215 6 2 352 96 470 4.71e-56 190
MsG0880043825.01.T01 MTR_4g015180 32.039 412 207 11 2 353 90 488 1.15e-52 181
MsG0880043825.01.T01 MTR_7g026770 48.469 196 73 3 54 247 132 301 4.73e-52 175
MsG0880043825.01.T01 MTR_5g081960 30.982 397 219 10 2 348 91 482 3.69e-51 177
MsG0880043825.01.T01 MTR_6g034485 61.832 131 44 2 99 229 1 125 4.65e-51 166
MsG0880043825.01.T01 MTR_3g102030 32.792 308 167 6 3 305 48 320 6.37e-48 164
MsG0880043825.01.T01 MTR_8g040760 66.102 118 39 1 2 118 95 212 2.77e-45 154
MsG0880043825.01.T01 MTR_4g094825 52.564 156 61 3 198 352 248 391 5.49e-45 159
MsG0880043825.01.T01 MTR_4g094825 69.318 88 27 0 1 88 160 247 2.66e-37 138
MsG0880043825.01.T01 MTR_6g045553 31.034 348 208 6 31 348 126 471 2.68e-44 159
MsG0880043825.01.T01 MTR_6g034495 68.041 97 29 1 2 96 71 167 9.01e-40 139
MsG0880043825.01.T01 MTR_7g026850 66.667 93 31 0 1 93 71 163 2.24e-38 135
MsG0880043825.01.T01 MTR_8g075610 25.356 351 235 9 2 340 77 412 1.69e-28 115
MsG0880043825.01.T01 MTR_7g026830 63.291 79 29 0 1 79 20 98 1.78e-28 107
MsG0880043825.01.T01 MTR_2g064495 27.035 344 227 12 2 333 86 417 1.23e-25 107
MsG0880043825.01.T01 MTR_4g007540 26.513 347 232 12 2 336 86 421 1.90e-25 106
MsG0880043825.01.T01 MTR_2g105330 27.035 344 227 12 2 333 86 417 5.15e-25 105
MsG0880043825.01.T01 MTR_7g026800 40.506 158 62 5 134 288 32 160 1.48e-23 96.7
MsG0880043825.01.T01 MTR_4g022780 40.000 160 64 5 134 290 33 163 3.95e-23 95.1
MsG0880043825.01.T01 MTR_7g026780 46.018 113 35 2 241 353 26 112 5.01e-23 92.8
MsG0880043825.01.T01 MTR_3g026950 27.915 283 166 9 50 301 129 404 6.23e-23 99.8
MsG0880043825.01.T01 MTR_8g096530 29.450 309 193 13 2 300 89 382 7.32e-23 99.4
MsG0880043825.01.T01 MTR_3g098980 26.384 307 198 11 6 301 100 389 5.63e-22 96.7
MsG0880043825.01.T01 MTR_3g049150 25.633 316 195 9 50 337 130 433 1.61e-21 95.5
MsG0880043825.01.T01 MTR_4g097090 25.796 314 189 9 50 333 141 440 1.38e-19 89.7
MsG0880043825.01.T01 MTR_4g127650 23.497 366 225 12 2 333 96 440 2.82e-19 89.0
MsG0880043825.01.T01 MTR_2g089765 26.389 288 163 10 50 300 159 434 3.66e-17 82.8
MsG0880043825.01.T01 MTR_7g026870 54.286 70 27 1 196 260 82 151 1.87e-16 76.3
MsG0880043825.01.T01 MTR_1g053315 26.531 343 225 17 6 333 115 445 3.92e-16 79.7
MsG0880043825.01.T01 MTR_7g086000 24.789 355 239 11 2 333 92 441 2.51e-15 77.4
MsG0880043825.01.T01 MTR_7g015860 27.476 313 175 14 45 324 141 434 2.88e-15 77.0
MsG0880043825.01.T01 MTR_1g040195 25.407 307 207 9 2 301 71 362 3.03e-15 77.0
MsG0880043825.01.T01 MTR_7g014250 26.061 330 196 13 30 325 122 437 1.05e-14 75.5
MsG0880043825.01.T01 MTR_2g089755 27.184 309 179 12 50 321 162 461 1.16e-14 75.1
MsG0880043825.01.T01 MTR_2g089650 28.826 281 159 13 50 303 154 420 1.18e-14 75.1
MsG0880043825.01.T01 MTR_2g089670 27.562 283 154 12 50 300 166 429 4.28e-14 73.6
MsG0880043825.01.T01 MTR_7g114490 23.889 360 238 13 2 342 95 437 5.77e-14 73.2
MsG0880043825.01.T01 MTR_1g081660 25.157 318 195 13 2 301 104 396 9.92e-14 72.4
MsG0880043825.01.T01 MTR_8g012750 27.244 312 173 14 30 300 124 422 3.45e-13 70.9
MsG0880043825.01.T01 MTR_7g084940 24.759 311 204 11 2 300 100 392 4.84e-13 70.1
MsG0880043825.01.T01 MTR_7g026880 54.545 55 22 1 298 352 2 53 9.93e-13 62.8
MsG0880043825.01.T01 MTR_7g014330 25.874 286 180 9 51 307 140 422 1.17e-12 68.9
MsG0880043825.01.T01 MTR_5g076700 24.127 315 201 12 2 299 95 388 1.47e-12 68.9
MsG0880043825.01.T01 MTR_4g055170 26.457 223 150 4 45 260 140 355 1.80e-12 68.6
MsG0880043825.01.T01 MTR_3g087640 26.935 323 186 13 50 339 157 462 2.15e-12 68.2
MsG0880043825.01.T01 MTR_7g084910 28.000 225 146 7 2 217 101 318 2.33e-12 68.2
MsG0880043825.01.T01 MTR_1g040215 24.620 329 210 13 2 315 94 399 2.91e-12 67.8
MsG0880043825.01.T01 MTR_3g049140 24.868 189 123 6 129 301 12 197 3.97e-12 65.9
MsG0880043825.01.T01 MTR_7g085930 22.222 378 228 13 2 328 89 451 6.33e-12 67.0
MsG0880043825.01.T01 MTR_7g056087 21.986 282 193 7 2 266 90 361 7.36e-12 66.6
MsG0880043825.01.T01 MTR_7g014230 26.269 335 185 13 32 325 126 439 9.10e-12 66.2
MsG0880043825.01.T01 MTR_6g015315 25.267 281 177 11 51 307 140 411 2.26e-11 65.1
MsG0880043825.01.T01 MTR_1g040175 23.885 314 201 12 2 299 94 385 4.08e-11 64.3
MsG0880043825.01.T01 MTR_7g014360 25.556 270 167 8 51 303 90 342 4.10e-11 64.3
MsG0880043825.01.T01 MTR_3g465980 27.568 185 127 4 52 231 147 329 4.37e-11 64.3
MsG0880043825.01.T01 MTR_2g089735 26.523 279 172 13 50 303 148 418 6.98e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043825.01.T01 AT5G67150 40.449 356 194 10 2 348 96 442 3.19e-83 260
MsG0880043825.01.T01 AT5G67160 41.737 357 188 10 2 352 90 432 6.09e-80 251
MsG0880043825.01.T01 AT3G50300 42.450 351 179 10 2 341 94 432 3.57e-79 249
MsG0880043825.01.T01 AT3G50280 39.155 355 201 7 2 349 94 440 2.27e-77 244
MsG0880043825.01.T01 AT3G50270 37.845 362 204 9 2 352 97 448 2.62e-74 237
MsG0880043825.01.T01 AT5G42830 36.842 361 208 8 2 352 99 449 1.02e-69 225
MsG0880043825.01.T01 AT3G50280 39.623 318 178 6 38 349 1 310 5.92e-69 219
MsG0880043825.01.T01 AT5G01210 35.385 390 203 9 1 352 91 469 2.76e-68 222
MsG0880043825.01.T01 AT5G07860 38.701 354 201 9 2 351 107 448 2.90e-68 221
MsG0880043825.01.T01 AT5G07850 37.950 361 204 8 2 353 105 454 1.06e-66 217
MsG0880043825.01.T01 AT5G38130 38.663 344 184 10 2 333 114 442 3.72e-64 211
MsG0880043825.01.T01 AT2G39980 33.333 402 206 8 2 353 92 481 4.00e-63 209
MsG0880043825.01.T01 AT5G07870 37.466 371 198 11 2 353 107 462 4.84e-62 205
MsG0880043825.01.T01 AT3G50290 44.161 274 135 7 35 298 4 269 2.96e-58 190
MsG0880043825.01.T01 AT5G23940 32.571 350 224 7 6 348 104 448 1.96e-51 178
MsG0880043825.01.T01 AT5G48930 28.198 344 230 12 2 336 86 421 5.50e-25 105
MsG0880043825.01.T01 AT2G19070 27.371 369 224 15 2 340 88 442 5.96e-22 97.1
MsG0880043825.01.T01 AT3G29590 24.079 353 251 10 3 343 95 442 6.96e-19 87.8
MsG0880043825.01.T01 AT3G48720 27.184 309 202 12 2 301 91 385 5.00e-18 85.1
MsG0880043825.01.T01 AT5G63560 25.484 310 202 12 2 301 91 381 1.13e-17 84.0
MsG0880043825.01.T01 AT5G39090 29.286 280 151 13 48 300 140 399 1.02e-16 81.3
MsG0880043825.01.T01 AT1G24420 25.691 362 236 16 2 352 90 429 7.82e-16 78.6
MsG0880043825.01.T01 AT1G24420 25.691 362 236 16 2 352 90 429 7.82e-16 78.6
MsG0880043825.01.T01 AT1G28680 25.660 265 181 8 45 303 135 389 8.77e-16 78.6
MsG0880043825.01.T01 AT2G40230 25.697 323 214 10 2 317 91 394 1.16e-15 78.2
MsG0880043825.01.T01 AT5G39050 25.263 285 169 10 52 303 149 422 1.09e-14 75.5
MsG0880043825.01.T01 AT1G78990 26.087 322 196 12 1 301 96 396 5.33e-14 73.2
MsG0880043825.01.T01 AT5G61160 23.988 321 212 12 48 343 139 452 5.34e-14 73.2
MsG0880043825.01.T01 AT1G28680 25.907 193 134 5 45 233 135 322 5.96e-14 72.8
MsG0880043825.01.T01 AT5G16410 24.308 325 196 12 1 301 121 419 3.26e-13 70.9
MsG0880043825.01.T01 AT3G29635 25.161 310 195 12 48 333 142 438 3.35e-13 70.9
MsG0880043825.01.T01 AT1G03390 26.891 357 208 18 6 333 111 443 5.35e-13 70.1
MsG0880043825.01.T01 AT1G27620 27.083 240 164 7 2 235 89 323 1.93e-12 68.6
MsG0880043825.01.T01 AT1G32910 24.398 332 194 12 1 301 94 399 1.31e-11 65.9
MsG0880043825.01.T01 AT4G15390 22.161 361 253 10 2 348 89 435 2.83e-11 64.7
MsG0880043825.01.T01 AT4G15390 22.161 361 253 10 2 348 89 435 2.83e-11 64.7

Find 60 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCCTGGTTTAATGTTTA+TGG 0.116946 8:-31232349 MsG0880043825.01.T01:CDS
ACGTTCAAATGATGGAATTT+TGG 0.223149 8:+31232912 None:intergenic
AATGAAACATCCTCTCATTT+AGG 0.241281 8:+31232785 None:intergenic
AATATTTGTTGTGTGTTTAA+AGG 0.258612 8:-31232262 MsG0880043825.01.T01:CDS
TGGTAATGATCCTGAGTTTA+TGG 0.261735 8:-31232179 MsG0880043825.01.T01:CDS
GGGAGTGAAAATAATGAAAA+TGG 0.264658 8:-31232286 MsG0880043825.01.T01:CDS
CCATTGAAAGAGGATTATTT+TGG 0.285934 8:-31232577 MsG0880043825.01.T01:CDS
CAAGTAACAGAACTACTTAA+TGG 0.339595 8:-31233039 MsG0880043825.01.T01:CDS
AATGTTTATGGTAATGATTT+TGG 0.340175 8:-31232337 MsG0880043825.01.T01:CDS
GTGCATTATGTGGTGGTTAT+AGG 0.349094 8:-31232622 MsG0880043825.01.T01:CDS
CATTAAGTAGTTCTGTTACT+TGG 0.357626 8:+31233040 None:intergenic
AGGTGTAGAAGAAGGTAGTA+TGG 0.364829 8:-31232242 MsG0880043825.01.T01:CDS
AATTGCCTCAAAAGTTTCAT+AGG 0.367209 8:+31232201 None:intergenic
CCAAAATAATCCTCTTTCAA+TGG 0.374799 8:+31232577 None:intergenic
TCGCTGATGGCTCATCGATT+TGG 0.384161 8:-31232984 MsG0880043825.01.T01:CDS
ACACTAGAAGCAACATTAGT+TGG 0.409033 8:+31232147 None:intergenic
TGGACTTGGTAAAGGTGCTT+TGG 0.409489 8:-31232497 MsG0880043825.01.T01:CDS
TGCGTGCACAGTTAATCATT+TGG 0.412364 8:-31233007 MsG0880043825.01.T01:CDS
TTTGGCATTTCATCAATTCA+TGG 0.451540 8:-31232966 MsG0880043825.01.T01:CDS
GGAAACCAACGTTCAAATGA+TGG 0.463001 8:+31232904 None:intergenic
TTTGGTTGGGGAAAACCTGT+GGG 0.472290 8:-31232319 MsG0880043825.01.T01:CDS
CTTTGTACCAGCTTCTAAGT+TGG 0.477040 8:+31232726 None:intergenic
ATTGTGAAAGGAAATTGAAT+CGG 0.485065 8:+31232865 None:intergenic
GTTGATTCCTCCATTGAAAG+AGG 0.486725 8:-31232587 MsG0880043825.01.T01:CDS
ATCGGAGGTTGAATACCATC+TGG 0.489177 8:+31232883 None:intergenic
GTGTTTAAAGGTGTAGAAGA+AGG 0.498123 8:-31232250 MsG0880043825.01.T01:CDS
ACCAGTGTCGATGACATCCT+TGG 0.503399 8:-31233153 MsG0880043825.01.T01:CDS
CAAGACAATGGACTTGGTAA+AGG 0.507284 8:-31232505 MsG0880043825.01.T01:CDS
TTGGCATTTCATCAATTCAT+GGG 0.510084 8:-31232965 MsG0880043825.01.T01:CDS
TTCAAAGCCAACTTAGAAGC+TGG 0.514017 8:-31232733 MsG0880043825.01.T01:CDS
GTTAATCATTTGGTCGCTGA+TGG 0.520192 8:-31232997 MsG0880043825.01.T01:CDS
GTTACTTGGACAGCAAGTAA+TGG 0.529511 8:+31233054 None:intergenic
TTCAATGGAGGAATCAACCT+TGG 0.531124 8:+31232592 None:intergenic
GTACAATAGTAAAACATTAG+TGG 0.537819 8:-31232380 MsG0880043825.01.T01:CDS
TACAATAGTAAAACATTAGT+GGG 0.542055 8:-31232379 MsG0880043825.01.T01:CDS
TCCGTTGTTAGCATGAAAGT+TGG 0.545219 8:-31232538 MsG0880043825.01.T01:CDS
AAATTCCATCATTTGAACGT+TGG 0.545341 8:-31232909 MsG0880043825.01.T01:CDS
GCACTTCTTCTCGAGGGTCA+AGG 0.553386 8:+31232641 None:intergenic
TTGGTTGGGGAAAACCTGTG+GGG 0.567130 8:-31232318 MsG0880043825.01.T01:CDS
TAGGATGATATGTGGCTCCA+AGG 0.569745 8:+31233136 None:intergenic
TTTGAACGTTGGTTTCCAGA+TGG 0.575241 8:-31232898 MsG0880043825.01.T01:CDS
TGGTTGGGGAAAACCTGTGG+GGG 0.578502 8:-31232317 MsG0880043825.01.T01:CDS
GTGAAAGGAAATTGAATCGG+AGG 0.583992 8:+31232868 None:intergenic
TCCAAGGATGTCATCGACAC+TGG 0.591435 8:+31233152 None:intergenic
AAATAATCCTCTTTCAATGG+AGG 0.592205 8:+31232580 None:intergenic
TTGTTGCAAGACAATGGACT+TGG 0.601437 8:-31232511 MsG0880043825.01.T01:CDS
TCCATCATGTGCAACAATGC+GGG 0.603295 8:-31233207 None:intergenic
ATACCAAGAGTAATAAAACT+AGG 0.607573 8:+31232403 None:intergenic
GGTGATTTGTTGCAAGACAA+TGG 0.609643 8:-31232517 MsG0880043825.01.T01:CDS
TGGTTATAGGCGTTAGACCA+AGG 0.625694 8:-31232609 MsG0880043825.01.T01:CDS
ACATAATGCACTTCTTCTCG+AGG 0.628027 8:+31232634 None:intergenic
TCGAGAAGAAGTGCATTATG+TGG 0.629418 8:-31232632 MsG0880043825.01.T01:CDS
GACACAACATCCATAAACTC+AGG 0.636566 8:+31232169 None:intergenic
AGAAGAAGTGCATTATGTGG+TGG 0.649545 8:-31232629 MsG0880043825.01.T01:CDS
CATAATGCACTTCTTCTCGA+GGG 0.661515 8:+31232635 None:intergenic
TAGTGGGTAGTAGTTCACCC+TGG 0.662355 8:-31232363 MsG0880043825.01.T01:CDS
ATTACCATAAACATTAAACC+AGG 0.672703 8:+31232345 None:intergenic
ACCAACTTTCATGCTAACAA+CGG 0.674514 8:+31232537 None:intergenic
TTCCATCATGTGCAACAATG+CGG 0.685385 8:-31233208 None:intergenic
TTACCATAAACATTAAACCA+GGG 0.701842 8:+31232346 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAATAGTAAAACATTAGT+GGG - Chr8:31232984-31233003 MsG0880043825.01.T01:CDS 20.0%
!!! AATATTTGTTGTGTGTTTAA+AGG - Chr8:31233101-31233120 MsG0880043825.01.T01:CDS 20.0%
!!! AATGTTTATGGTAATGATTT+TGG - Chr8:31233026-31233045 MsG0880043825.01.T01:CDS 20.0%
!!! TTAGAATGATGATTATTTTG+TGG + Chr8:31232528-31232547 None:intergenic 20.0%
! ATACCAAGAGTAATAAAACT+AGG + Chr8:31232963-31232982 None:intergenic 25.0%
! ATTACCATAAACATTAAACC+AGG + Chr8:31233021-31233040 None:intergenic 25.0%
! ATTGTGAAAGGAAATTGAAT+CGG + Chr8:31232501-31232520 None:intergenic 25.0%
! GTACAATAGTAAAACATTAG+TGG - Chr8:31232983-31233002 MsG0880043825.01.T01:CDS 25.0%
! TTACCATAAACATTAAACCA+GGG + Chr8:31233020-31233039 None:intergenic 25.0%
!! AAAAATGAGTGCAGAATTTT+AGG + Chr8:31232249-31232268 None:intergenic 25.0%
!!! CACTCATTTTTTCCATTTAA+CGG - Chr8:31232258-31232277 MsG0880043825.01.T01:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG - Chr8:31233031-31233050 MsG0880043825.01.T01:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG - Chr8:31233030-31233049 MsG0880043825.01.T01:CDS 25.0%
AAAAACTCAACCTAAATGAG+AGG - Chr8:31232568-31232587 MsG0880043825.01.T01:CDS 30.0%
AAATAATCCTCTTTCAATGG+AGG + Chr8:31232786-31232805 None:intergenic 30.0%
AAATTCCATCATTTGAACGT+TGG - Chr8:31232454-31232473 MsG0880043825.01.T01:CDS 30.0%
AATGAAACATCCTCTCATTT+AGG + Chr8:31232581-31232600 None:intergenic 30.0%
AATTGCCTCAAAAGTTTCAT+AGG + Chr8:31233165-31233184 None:intergenic 30.0%
CAAGTAACAGAACTACTTAA+TGG - Chr8:31232324-31232343 MsG0880043825.01.T01:CDS 30.0%
CCAAAATAATCCTCTTTCAA+TGG + Chr8:31232789-31232808 None:intergenic 30.0%
GGGAGTGAAAATAATGAAAA+TGG - Chr8:31233077-31233096 MsG0880043825.01.T01:CDS 30.0%
TGAAAAATGACTATGAGAGT+TGG - Chr8:31232928-31232947 MsG0880043825.01.T01:CDS 30.0%
TTGGCATTTCATCAATTCAT+GGG - Chr8:31232398-31232417 MsG0880043825.01.T01:CDS 30.0%
TTTGGCATTTCATCAATTCA+TGG - Chr8:31232397-31232416 MsG0880043825.01.T01:CDS 30.0%
! CATTAAGTAGTTCTGTTACT+TGG + Chr8:31232326-31232345 None:intergenic 30.0%
! CCATTGAAAGAGGATTATTT+TGG - Chr8:31232786-31232805 MsG0880043825.01.T01:CDS 30.0%
! TAGTTTTTCACTCCGTTAAA+TGG + Chr8:31232273-31232292 None:intergenic 30.0%
! TTTGTCCTATGAAACTTTTG+AGG - Chr8:31233157-31233176 MsG0880043825.01.T01:CDS 30.0%
!! AAGACAACCATTTGAGTTTT+TGG + Chr8:31232424-31232443 None:intergenic 30.0%
!! ACGTTCAAATGATGGAATTT+TGG + Chr8:31232454-31232473 None:intergenic 30.0%
!! TTTTGTGGTTCTATTGTGAA+AGG + Chr8:31232513-31232532 None:intergenic 30.0%
!!! ACACCTAGTTTTATTACTCT+TGG - Chr8:31232957-31232976 MsG0880043825.01.T01:CDS 30.0%
!!! CAGAATTTTAGGATGATATG+TGG + Chr8:31232238-31232257 None:intergenic 30.0%
!!! TATGAAACTTTTGAGGCAAT+TGG - Chr8:31233164-31233183 MsG0880043825.01.T01:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG - Chr8:31233032-31233051 MsG0880043825.01.T01:CDS 30.0%
ACCAACTTTCATGCTAACAA+CGG + Chr8:31232829-31232848 None:intergenic 35.0%
TCACCCTGGTTTAATGTTTA+TGG - Chr8:31233014-31233033 MsG0880043825.01.T01:CDS 35.0%
! AAGCACTTTTCACTCACATT+TGG - Chr8:31232676-31232695 MsG0880043825.01.T01:CDS 35.0%
! GTGTTTAAAGGTGTAGAAGA+AGG - Chr8:31233113-31233132 MsG0880043825.01.T01:CDS 35.0%
! TGGTAATGATCCTGAGTTTA+TGG - Chr8:31233184-31233203 MsG0880043825.01.T01:CDS 35.0%
AACGGAGTGAAAAACTACGA+AGG - Chr8:31232276-31232295 MsG0880043825.01.T01:CDS 40.0%
ACATAATGCACTTCTTCTCG+AGG + Chr8:31232732-31232751 None:intergenic 40.0%
ACGGAGTGAAAAACTACGAA+GGG - Chr8:31232277-31232296 MsG0880043825.01.T01:CDS 40.0%
AGGTGTAGAAGAAGGTAGTA+TGG - Chr8:31233121-31233140 MsG0880043825.01.T01:CDS 40.0%
CATAATGCACTTCTTCTCGA+GGG + Chr8:31232731-31232750 None:intergenic 40.0%
CTTTGTACCAGCTTCTAAGT+TGG + Chr8:31232640-31232659 None:intergenic 40.0%
GACACAACATCCATAAACTC+AGG + Chr8:31233197-31233216 None:intergenic 40.0%
GGAAACCAACGTTCAAATGA+TGG + Chr8:31232462-31232481 None:intergenic 40.0%
GTGAAAGGAAATTGAATCGG+AGG + Chr8:31232498-31232517 None:intergenic 40.0%
GTGCATTATGTGGTGGTTAT+AGG - Chr8:31232741-31232760 MsG0880043825.01.T01:CDS 40.0%
GTTAATCATTTGGTCGCTGA+TGG - Chr8:31232366-31232385 MsG0880043825.01.T01:CDS 40.0%
GTTACTTGGACAGCAAGTAA+TGG + Chr8:31232312-31232331 None:intergenic 40.0%
TCATGGGCCAAAAACTCAAA+TGG - Chr8:31232414-31232433 MsG0880043825.01.T01:CDS 40.0%
TCCGTTGTTAGCATGAAAGT+TGG - Chr8:31232825-31232844 MsG0880043825.01.T01:CDS 40.0%
TGCGTGCACAGTTAATCATT+TGG - Chr8:31232356-31232375 MsG0880043825.01.T01:CDS 40.0%
TTCAAAGCCAACTTAGAAGC+TGG - Chr8:31232630-31232649 MsG0880043825.01.T01:CDS 40.0%
TTCAATGGAGGAATCAACCT+TGG + Chr8:31232774-31232793 None:intergenic 40.0%
! AGAAGAAGTGCATTATGTGG+TGG - Chr8:31232734-31232753 MsG0880043825.01.T01:CDS 40.0%
! CAAGACAATGGACTTGGTAA+AGG - Chr8:31232858-31232877 MsG0880043825.01.T01:CDS 40.0%
! GTTGATTCCTCCATTGAAAG+AGG - Chr8:31232776-31232795 MsG0880043825.01.T01:CDS 40.0%
! TCGAGAAGAAGTGCATTATG+TGG - Chr8:31232731-31232750 MsG0880043825.01.T01:CDS 40.0%
! TTGTTGCAAGACAATGGACT+TGG - Chr8:31232852-31232871 MsG0880043825.01.T01:CDS 40.0%
!! GGTGATTTGTTGCAAGACAA+TGG - Chr8:31232846-31232865 MsG0880043825.01.T01:CDS 40.0%
!! TTTGAACGTTGGTTTCCAGA+TGG - Chr8:31232465-31232484 MsG0880043825.01.T01:CDS 40.0%
ATCGGAGGTTGAATACCATC+TGG + Chr8:31232483-31232502 None:intergenic 45.0%
TAGGATGATATGTGGCTCCA+AGG + Chr8:31232230-31232249 None:intergenic 45.0%
TGGTTATAGGCGTTAGACCA+AGG - Chr8:31232754-31232773 MsG0880043825.01.T01:CDS 45.0%
TTTGGTTGGGGAAAACCTGT+GGG - Chr8:31233044-31233063 MsG0880043825.01.T01:CDS 45.0%
! TTCTCGAGGGTCAAGGTTTT+TGG + Chr8:31232718-31232737 None:intergenic 45.0%
!! TGGACTTGGTAAAGGTGCTT+TGG - Chr8:31232866-31232885 MsG0880043825.01.T01:CDS 45.0%
!! TTTTGGTTGGGGAAAACCTG+TGG - Chr8:31233043-31233062 MsG0880043825.01.T01:CDS 45.0%
TCCAAGGATGTCATCGACAC+TGG + Chr8:31232214-31232233 None:intergenic 50.0%
TTGGTTGGGGAAAACCTGTG+GGG - Chr8:31233045-31233064 MsG0880043825.01.T01:CDS 50.0%
! TAGTGGGTAGTAGTTCACCC+TGG - Chr8:31233000-31233019 MsG0880043825.01.T01:CDS 50.0%
! TCGCTGATGGCTCATCGATT+TGG - Chr8:31232379-31232398 MsG0880043825.01.T01:CDS 50.0%
!! ACCAGTGTCGATGACATCCT+TGG - Chr8:31232210-31232229 MsG0880043825.01.T01:CDS 50.0%
AAACCTGTGGGGGTTCGAAC+TGG - Chr8:31233056-31233075 MsG0880043825.01.T01:CDS 55.0%
AACCTGTGGGGGTTCGAACT+GGG - Chr8:31233057-31233076 MsG0880043825.01.T01:CDS 55.0%
GCACTTCTTCTCGAGGGTCA+AGG + Chr8:31232725-31232744 None:intergenic 55.0%
TGGTTGGGGAAAACCTGTGG+GGG - Chr8:31233046-31233065 MsG0880043825.01.T01:CDS 55.0%
CTCCCAGTTCGAACCCCCAC+AGG + Chr8:31233062-31233081 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 31232162 31233223 31232162 ID=MsG0880043825.01;Name=MsG0880043825.01
Chr8 mRNA 31232162 31233223 31232162 ID=MsG0880043825.01.T01;Parent=MsG0880043825.01;Name=MsG0880043825.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|353
Chr8 exon 31232162 31233223 31232162 ID=MsG0880043825.01.T01:exon:7514;Parent=MsG0880043825.01.T01
Chr8 CDS 31232162 31233223 31232162 ID=MsG0880043825.01.T01:cds;Parent=MsG0880043825.01.T01
Gene Sequence

>MsG0880043825.01.T01

ATGTGCAACAATGCGGGTGCACTCTTTGTTCATGCTGCAGCAGAAAATACCAGTGTCGATGACATCCTTGGAGCCACATATCATCCTAAAATTCTGCACTCATTTTTTCCATTTAACGGAGTGAAAAACTACGAAGGGACATCAAAGCCATTACTTGCTGTCCAAGTAACAGAACTACTTAATGGCATCTTCATTGCGTGCACAGTTAATCATTTGGTCGCTGATGGCTCATCGATTTGGCATTTCATCAATTCATGGGCCAAAAACTCAAATGGTTGTCTTGAAATATCCAAAATTCCATCATTTGAACGTTGGTTTCCAGATGGTATTCAACCTCCGATTCAATTTCCTTTCACAATAGAACCACAAAATAATCATCATTCTAATAATGATTATCATAAAGAAGAAAAACTCAACCTAAATGAGAGGATGTTTCATTTCACAAAAGAGAATATTGCAAAACTAAAATTCAAAGCCAACTTAGAAGCTGGTACAAAGAACATATCTTCTTTGCAAGCACTTTTCACTCACATTTGGCGATCTATTATACGTTCCAAAAACCTTGACCCTCGAGAAGAAGTGCATTATGTGGTGGTTATAGGCGTTAGACCAAGGTTGATTCCTCCATTGAAAGAGGATTATTTTGGTAACGCTATGATAGACTCCGTTGTTAGCATGAAAGTTGGTGATTTGTTGCAAGACAATGGACTTGGTAAAGGTGCTTTGGAGATGAACAAGATGATTGCTTTGCAATCTGATGAGATGTTGAAAAATGACTATGAGAGTTGGTTGAAAACACCTAGTTTTATTACTCTTGGTATGTACAATAGTAAAACATTAGTGGGTAGTAGTTCACCCTGGTTTAATGTTTATGGTAATGATTTTGGTTGGGGAAAACCTGTGGGGGTTCGAACTGGGAGTGAAAATAATGAAAATGGAAATATTTGTTGTGTGTTTAAAGGTGTAGAAGAAGGTAGTATGGATCTTGAAGTGTGTTTGTCCTATGAAACTTTTGAGGCAATTGGTAATGATCCTGAGTTTATGGATGTTGTGTCCAACTAA
Protein sequence

>MsG0880043825.01.T01

MCNNAGALFVHAAAENTSVDDILGATYHPKILHSFFPFNGVKNYEGTSKPLLAVQVTELLNGIFIACTVNHLVADGSSIWHFINSWAKNSNGCLEISKIPSFERWFPDGIQPPIQFPFTIEPQNNHHSNNDYHKEEKLNLNERMFHFTKENIAKLKFKANLEAGTKNISSLQALFTHIWRSIIRSKNLDPREEVHYVVVIGVRPRLIPPLKEDYFGNAMIDSVVSMKVGDLLQDNGLGKGALEMNKMIALQSDEMLKNDYESWLKTPSFITLGMYNSKTLVGSSSPWFNVYGNDFGWGKPVGVRTGSENNENGNICCVFKGVEEGSMDLEVCLSYETFEAIGNDPEFMDVVSN*